HG10011496 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10011496
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description65-kDa microtubule-associated protein 1-like
LocationChr01: 6588403 .. 6592394 (-)
RNA-Seq ExpressionHG10011496
SyntenyHG10011496
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGTGGCAGATACACAAAATCCTCTTCAAGGAGAAACCACATGCGGTACCCTACTGCAGAAACTGCAAGTAAATAAATCACGCTGTTGATCTTTTGTTTCCTCTTTTCCATCTAAATTTTTTCTATTGGACTGACCGAAGATTACCTTAGGAAATTTGGGATGAAGTTGGTGAAAGTGATGAGGAACGAGATAAAATGCTGCTTCAGATAGATCAAGAATGTTTGGATGTCTACAAAAGGAAAGTTGAGCAAGCTGATAAGTCAAGGGCAAAACTTTTGCAGTCTTTATCAGATGCAAAGCATGAGCTTTCCAGTCTTCTATCTGCTCTTGGTGAAAAAAGTTTTTCTGAATTTGTGAGTAAATAATTTATATAATCTCGTTTATATCTGAAAATGCTGATGCATTTGTCTGGTGATTCATATCAAGTTCTATTCTCTTCTTTTAATTACAGCCTGAGAAGGCTGCTGGAACAATCAAAGAACAGCTTGCAGCCATAGCTCCAGCTCTCGAACAGCTTTGGAAACAGAAAGAGAAGAGGGTTAAGGAATTTTTTGATGTACAATCAGAGATTCAGAAGATATGTGGGGAGATTGCTGGGAGTTTAAACCTTAGCGAGAATCCAGTAGTAGATGAATCTCAGCTTTCGGTAGAAAAGCTGGACGAATACCATTGCCAACTTCAGGAACTGCAGAAAGAAAAGGTGAAGTTCTATTTTGGGGAGCAATCTATTTATTTATCTTTCGTCCTTCATCAAGCCAATCTTTAATCATTTATTCAAATACGAACAGAGTGAAAGGTTGCACAAAGTTCTTGAATTTGTGAGCACCGTGCATGATCTGTGTGCTGTCCTTGGAATTGATTTCTTTGCTACTGTAACTGAGGTTCACCCAAGCTTAAATGACGCAACTGGTGTGCAATCTAAAAGCATTAGCAACGATACTTTATCCAAGCTGGCAAAGATGGTCTTAGCACTAAAAGAAGATAAAAAGCAAAGGCTACATAAGGTAAATTTAATATTCTGGACTTGTTATTATAAAGGTGAGTTTTGGGCACCTTCTAATTTGAACACTTCTTAAAAGTATATATTTTTCTTAAAACGCTATTTATAAAAATTGTTTTAACTAAAACATTTATGTTTTTCTCTAATCAGAAATTCATTTGGTTGACCTACATCTGAAGTGAATGATTCTGCAAATGCTAAATTAGCACGAAATACTCCTAAAGTGATTTACAATCGACTACATTATATTCACTTTTTGGATAATCAACTTCAAAATGATTCTTCAGAAAATGATTTAAAACCAATTTAATGATTTCAAAAGTGATTTTTATTCATTACCAAACACTTCAGATTCTTTAATGAACATGTTAGATCCTCCAAAGTTACTGAAGAAGTAGATCCAAAAACACTCTAAAGAAGAGAATTTCAAAATTACATTTGCTTTGCTTCCCTGGTGCAAAATATGAAAACGGGCTTTATTTGATGTGAAACTTATGCGTTAGTATCTGTATCATTCATGTTTTACCATTTAAAATGATATTTAATTGAACTTTTTTATAGTATTCATCTAGTCTAAAATTTTAAAAGTTGAAATCTTCCCACTAAAATGTATCTGTCATTTGTATCATCAAAATGGTATTAGAATTACAATTACCTGTGGTATGGCATCTAGATTGAATTTATTGCTTTATTAGTCAACACTAAAGTGAGAACAGATGTATGCCATACACTCTTGACTGTTTCTTAGCTGGGTATGTCTTTTCTCCTCTTTATGCAACTAGTACATATAGCAACGTATGGGAAACTTGAGCTTTTTAGAGCTTGAACCAATGCATGGGCAACTCTACGATATTTCAACAGTACTTGTATCTGTCAGTTGTAGGGGTAAAGATAACCTTTATTTTGTGCATTCTCATACATACTAGAGCTTGCATTATTTTTACTTCTAGATTATGATGTTGTGATATTGCCAAGGTGAAGTACCATGTATTTCTTCAGTTTACTTGTCTGTTTGGAGCTTTTTAACTTTGTATTATTTTGTATTTTGTCATATACAGCTTCAAGAATTGGCAACTCAGCTGATTGATCTTTGGAATTTGATGGATACCCCCTCAGAAGAAAGAAGTTTATTTGACCATGTCACGTGTAATATATCAGCATCAGTAGATGAAGTGACTGTCCCAGGGGCTCTTGCTCTTGATTTGATTGAGCAGGTATTTGAGCCTTCTGTAGATATCTTGCTGTAAATTTGAATAGGTGTGGTTGTTGACTGTTTTTTTTGTACATGTTAAATTTACAAGGCTGAGGTGGAGGTTGAGAGGCTTGATCAACTCAAGGCTAGTAGGATGAAGGAAATTGCTTTCAAGAAGCAATCTGAACTTGATGAGATATTTTCTCATGCTCACATAGAAATAGACCCAGAGGCAGCACGAGAAAAAATTATGGCACTGATTGATTCTGGTAATGTTGAGCCTGCTGAATTACTGGATGACATGGACGATCAAATAGCAAAGGCCAAAGAAGAAGTTCTCAGCAGGAAAGAAATCTTAGACAAGGTTGAAAAATGGATGTTGGCATGTGAAGAAGAGAGCTGGCTAGAAGACTACAATCGGGTACTTGTCCTTTCCAGCTATTGTATTAAAAATATTAGCTAGTATAAAAATTTCGATTAAGTTGATTTGCCCATATAAACTCTTTGATTTCAGGAAATTTTGCACTGATTGCCAGTTTATAATTTTCTGTGGAATTGCCATTTCGTAACTCCCTGGACTGTTTTTAGTCTGACATGGATTTCATTAGTTAAATTTCTTAATTTACTGAACATGGCTAGACACTCGATGTATATGTATGTATGTATGTATGTATGTTATTGTACATAGAACCCATAATATCCAACACTTGTTATAGATTTCAATGCCTGGCAGGATGTTGTTGAAAGCAGCCATGGAAGATTCGTTTCTGCATGGCTGCAGTTACTTGGTAAAGTTACAGATCTAAAATGTGGACTTGCTTTCCAAAAAATTAACTGGAAAACTAGCATAAATTTACTTCTTACGATGGTTGATAAAAGTTCTAGGATTGGCAACTGATACATCATGTCAGTTTCACAGTTGAAACTTTGAATGCCACTCCAAAAGACTCGGACTAAAATGTATGTGCTACTTTTAATTTCTCAAAAATACTGGACTTGTCTTGTGTTGTGTTTTGACACCATTGTGGCGTCACCTTGCAGGATGATAATAGGTACAATGCTAGCAGAGGGGCTCATTTGAATCTCAAACGTGCAGAGAAAGCTAGAATTCTGGTCAACAAAATTCCAGGTTTGTATTAAAAACATACGAGGACTTCAATATTCTCCTTACGATTCCCTGGATACTAATGCTTGGTTCTTCTTTATGGGCTTGGATATGTAGCTCTTGTTGACACTTTAATTGCCAAAACTCGGGCTTGGGAGGAAGATCGTGGCATATCGTTTATTTACGACGGTGTTCCTCTCCTTGCTATGCTAGACGAATATACCATGCTCAGGCATGAAAGAGAGGAAGAAAAACGACGATTGAGGGTAAATTGTTTCAAACCTCTTGATATTTATGAACTTATTCATGCACATGTTACTTTCGGTTTGACGTTGCCTCAAACAAACACCCTCTTTTTTTGCCCTTAATGGCCTCTTACGGACTTTGCAGGACCATAAAAAATTTCATGAGCAGCAAACCACAGACCCAGAGATCATCACCAACTCAAGGCCTAGCCCTGCTCGACCCATTGGTACAAAGAAGGCAGCGGGGCCTCGTGCGAATGGAGCTGCAAACGGAACACCTAGCAGCAGACGGTTATCGCTTAATCAAAATGGATCTAGATCAATGAACAAAGATGGAAAAAGGGACAGTATGAGACCAATGACACCCGTGAAATACGATGCAATCTCGAAAGAAGACTCTGCCTCACACATTTCAGGTACCGAACCTGTTTCATCTTCACCATGA

mRNA sequence

ATGGCGGTGGCAGATACACAAAATCCTCTTCAAGGAGAAACCACATGCGGTACCCTACTGCAGAAACTGCAAGAAATTTGGGATGAAGTTGGTGAAAGTGATGAGGAACGAGATAAAATGCTGCTTCAGATAGATCAAGAATGTTTGGATGTCTACAAAAGGAAAGTTGAGCAAGCTGATAAGTCAAGGGCAAAACTTTTGCAGTCTTTATCAGATGCAAAGCATGAGCTTTCCAGTCTTCTATCTGCTCTTGGTGAAAAAAGTTTTTCTGAATTTCCTGAGAAGGCTGCTGGAACAATCAAAGAACAGCTTGCAGCCATAGCTCCAGCTCTCGAACAGCTTTGGAAACAGAAAGAGAAGAGGGTTAAGGAATTTTTTGATGTACAATCAGAGATTCAGAAGATATGTGGGGAGATTGCTGGGAGTTTAAACCTTAGCGAGAATCCAGTAGTAGATGAATCTCAGCTTTCGGTAGAAAAGCTGGACGAATACCATTGCCAACTTCAGGAACTGCAGAAAGAAAAGAGTGAAAGGTTGCACAAAGTTCTTGAATTTGTGAGCACCGTGCATGATCTGTGTGCTGTCCTTGGAATTGATTTCTTTGCTACTGTAACTGAGGTTCACCCAAGCTTAAATGACGCAACTGGTGTGCAATCTAAAAGCATTAGCAACGATACTTTATCCAAGCTGGCAAAGATGGTCTTAGCACTAAAAGAAGATAAAAAGCAAAGGCTACATAAGCTTCAAGAATTGGCAACTCAGCTGATTGATCTTTGGAATTTGATGGATACCCCCTCAGAAGAAAGAAGTTTATTTGACCATGTCACGTGTAATATATCAGCATCAGTAGATGAAGTGACTGTCCCAGGGGCTCTTGCTCTTGATTTGATTGAGCAGGCTGAGGTGGAGGTTGAGAGGCTTGATCAACTCAAGGCTAGTAGGATGAAGGAAATTGCTTTCAAGAAGCAATCTGAACTTGATGAGATATTTTCTCATGCTCACATAGAAATAGACCCAGAGGCAGCACGAGAAAAAATTATGGCACTGATTGATTCTGGTAATGTTGAGCCTGCTGAATTACTGGATGACATGGACGATCAAATAGCAAAGGCCAAAGAAGAAGTTCTCAGCAGGAAAGAAATCTTAGACAAGGTTGAAAAATGGATGTTGGCATGTGAAGAAGAGAGCTGGCTAGAAGACTACAATCGGATTTCAATGCCTGGCAGGATGTTGTTGAAAGCAGCCATGGAAGATTCGTTTCTGCATGGCTGCAGTTACTTGGATGATAATAGGTACAATGCTAGCAGAGGGGCTCATTTGAATCTCAAACGTGCAGAGAAAGCTAGAATTCTGGTCAACAAAATTCCAGCTCTTGTTGACACTTTAATTGCCAAAACTCGGGCTTGGGAGGAAGATCGTGGCATATCGTTTATTTACGACGGTGTTCCTCTCCTTGCTATGCTAGACGAATATACCATGCTCAGGCATGAAAGAGAGGAAGAAAAACGACGATTGAGGGACCATAAAAAATTTCATGAGCAGCAAACCACAGACCCAGAGATCATCACCAACTCAAGGCCTAGCCCTGCTCGACCCATTGGTACAAAGAAGGCAGCGGGGCCTCGTGCGAATGGAGCTGCAAACGGAACACCTAGCAGCAGACGGTTATCGCTTAATCAAAATGGATCTAGATCAATGAACAAAGATGGAAAAAGGGACAGTATGAGACCAATGACACCCGTGAAATACGATGCAATCTCGAAAGAAGACTCTGCCTCACACATTTCAGGTACCGAACCTGTTTCATCTTCACCATGA

Coding sequence (CDS)

ATGGCGGTGGCAGATACACAAAATCCTCTTCAAGGAGAAACCACATGCGGTACCCTACTGCAGAAACTGCAAGAAATTTGGGATGAAGTTGGTGAAAGTGATGAGGAACGAGATAAAATGCTGCTTCAGATAGATCAAGAATGTTTGGATGTCTACAAAAGGAAAGTTGAGCAAGCTGATAAGTCAAGGGCAAAACTTTTGCAGTCTTTATCAGATGCAAAGCATGAGCTTTCCAGTCTTCTATCTGCTCTTGGTGAAAAAAGTTTTTCTGAATTTCCTGAGAAGGCTGCTGGAACAATCAAAGAACAGCTTGCAGCCATAGCTCCAGCTCTCGAACAGCTTTGGAAACAGAAAGAGAAGAGGGTTAAGGAATTTTTTGATGTACAATCAGAGATTCAGAAGATATGTGGGGAGATTGCTGGGAGTTTAAACCTTAGCGAGAATCCAGTAGTAGATGAATCTCAGCTTTCGGTAGAAAAGCTGGACGAATACCATTGCCAACTTCAGGAACTGCAGAAAGAAAAGAGTGAAAGGTTGCACAAAGTTCTTGAATTTGTGAGCACCGTGCATGATCTGTGTGCTGTCCTTGGAATTGATTTCTTTGCTACTGTAACTGAGGTTCACCCAAGCTTAAATGACGCAACTGGTGTGCAATCTAAAAGCATTAGCAACGATACTTTATCCAAGCTGGCAAAGATGGTCTTAGCACTAAAAGAAGATAAAAAGCAAAGGCTACATAAGCTTCAAGAATTGGCAACTCAGCTGATTGATCTTTGGAATTTGATGGATACCCCCTCAGAAGAAAGAAGTTTATTTGACCATGTCACGTGTAATATATCAGCATCAGTAGATGAAGTGACTGTCCCAGGGGCTCTTGCTCTTGATTTGATTGAGCAGGCTGAGGTGGAGGTTGAGAGGCTTGATCAACTCAAGGCTAGTAGGATGAAGGAAATTGCTTTCAAGAAGCAATCTGAACTTGATGAGATATTTTCTCATGCTCACATAGAAATAGACCCAGAGGCAGCACGAGAAAAAATTATGGCACTGATTGATTCTGGTAATGTTGAGCCTGCTGAATTACTGGATGACATGGACGATCAAATAGCAAAGGCCAAAGAAGAAGTTCTCAGCAGGAAAGAAATCTTAGACAAGGTTGAAAAATGGATGTTGGCATGTGAAGAAGAGAGCTGGCTAGAAGACTACAATCGGATTTCAATGCCTGGCAGGATGTTGTTGAAAGCAGCCATGGAAGATTCGTTTCTGCATGGCTGCAGTTACTTGGATGATAATAGGTACAATGCTAGCAGAGGGGCTCATTTGAATCTCAAACGTGCAGAGAAAGCTAGAATTCTGGTCAACAAAATTCCAGCTCTTGTTGACACTTTAATTGCCAAAACTCGGGCTTGGGAGGAAGATCGTGGCATATCGTTTATTTACGACGGTGTTCCTCTCCTTGCTATGCTAGACGAATATACCATGCTCAGGCATGAAAGAGAGGAAGAAAAACGACGATTGAGGGACCATAAAAAATTTCATGAGCAGCAAACCACAGACCCAGAGATCATCACCAACTCAAGGCCTAGCCCTGCTCGACCCATTGGTACAAAGAAGGCAGCGGGGCCTCGTGCGAATGGAGCTGCAAACGGAACACCTAGCAGCAGACGGTTATCGCTTAATCAAAATGGATCTAGATCAATGAACAAAGATGGAAAAAGGGACAGTATGAGACCAATGACACCCGTGAAATACGATGCAATCTCGAAAGAAGACTCTGCCTCACACATTTCAGGTACCGAACCTGTTTCATCTTCACCATGA

Protein sequence

MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQADKSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEKRVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLHKVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKEDKKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQAEVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAELLDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSFLHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAGPRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKEDSASHISGTEPVSSSP
Homology
BLAST of HG10011496 vs. NCBI nr
Match: XP_038905625.1 (65-kDa microtubule-associated protein 1-like [Benincasa hispida])

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 574/605 (94.88%), Postives = 578/605 (95.54%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD
Sbjct: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEK +GTIKEQLAAIAPALEQLWKQKE+
Sbjct: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKDSGTIKEQLAAIAPALEQLWKQKEQ 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEIAGSLNLS+NPVVDESQLSVEKLDEYH QLQELQKEKSERLH
Sbjct: 121 RVKEFFDVQSEIQKICGEIAGSLNLSDNPVVDESQLSVEKLDEYHSQLQELQKEKSERLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360
           EVEVERLDQLKASRMKEIAFKKQSEL+EIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL
Sbjct: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360

Query: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSF 420
           LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR                 
Sbjct: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR----------------- 420

Query: 421 LHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480
                  DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD
Sbjct: 421 -------DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480

Query: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG 540
           GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTD EIITNSRPSPARPIGTKKAAG
Sbjct: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDQEIITNSRPSPARPIGTKKAAG 540

Query: 541 PRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKEDSASHISGTEP 600
           PRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKEDSASHISGTEP
Sbjct: 541 PRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKEDSASHISGTEP 581

Query: 601 VSSSP 606
           VSSSP
Sbjct: 601 VSSSP 581

BLAST of HG10011496 vs. NCBI nr
Match: XP_011650516.1 (65-kDa microtubule-associated protein 1 [Cucumis sativus] >KGN56090.1 hypothetical protein Csa_009760 [Cucumis sativus])

HSP 1 Score: 1055.4 bits (2728), Expect = 1.8e-304
Identity = 562/605 (92.89%), Postives = 571/605 (94.38%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAVADTQ PLQGETTCGTLLQKLQEIWDEVGE+DEERDKMLLQIDQECLDVYKRKVE AD
Sbjct: 1   MAVADTQIPLQGETTCGTLLQKLQEIWDEVGETDEERDKMLLQIDQECLDVYKRKVELAD 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKA+GTIKEQLAAIAPALE+LWKQKE+
Sbjct: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKASGTIKEQLAAIAPALEKLWKQKEQ 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKS+RLH
Sbjct: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSDRLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360
           EVEVERLDQLKASRMKEIAFKKQSEL+EIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL
Sbjct: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360

Query: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSF 420
           LDDMDDQI KAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR                 
Sbjct: 361 LDDMDDQITKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR----------------- 420

Query: 421 LHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480
                  DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD
Sbjct: 421 -------DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480

Query: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG 540
           GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFH+QQTTDPEIITNSRPSP R I TKKA G
Sbjct: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHDQQTTDPEIITNSRPSPVRTIATKKAGG 540

Query: 541 PRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKEDSASHISGTEP 600
            RANGAANGTPSSRRLSLNQNGSRSMNKD KRDSMRPMTPVKYDAISK+DS SHISGTEP
Sbjct: 541 ARANGAANGTPSSRRLSLNQNGSRSMNKDSKRDSMRPMTPVKYDAISKDDSGSHISGTEP 581

Query: 601 VSSSP 606
           VS+SP
Sbjct: 601 VSASP 581

BLAST of HG10011496 vs. NCBI nr
Match: KAG7028419.1 (65-kDa microtubule-associated protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1053.5 bits (2723), Expect = 6.9e-304
Identity = 557/605 (92.07%), Postives = 569/605 (94.05%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAV DTQNPLQGETTCGTLLQKLQEIWDEVGESDE+RDKMLLQIDQECLDVYKRKVE AD
Sbjct: 1   MAVEDTQNPLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVELAD 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAKHELS+LLSALGEKSFSEFPE A+GTIKEQLAAIAPALEQLWKQKEK
Sbjct: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEI GSLN+SENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH
Sbjct: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFF TVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQL+DLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLVDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360
           EVEVERLDQLKASRMKEIAFKKQSEL+EIF+HAHIEIDPEAAREKIM LIDSGNVEP+EL
Sbjct: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIDPEAAREKIMTLIDSGNVEPSEL 360

Query: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSF 420
           LDDMD++IAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR                 
Sbjct: 361 LDDMDNKIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR----------------- 420

Query: 421 LHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480
                  DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD
Sbjct: 421 -------DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480

Query: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG 540
           GVPLLAMLDEYTMLRHEREEEKRR RDHKKFHEQQ+TD E ITNSRPSPARPIGTKKAAG
Sbjct: 481 GVPLLAMLDEYTMLRHEREEEKRRFRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAG 540

Query: 541 PRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKEDSASHISGTEP 600
           PRANGA NGTPSSRRLSLNQNGSRSMNKDGKRD MRPMTPVKYDAISKEDS SH+SGTEP
Sbjct: 541 PRANGAVNGTPSSRRLSLNQNGSRSMNKDGKRDGMRPMTPVKYDAISKEDSGSHVSGTEP 581

Query: 601 VSSSP 606
           VSSSP
Sbjct: 601 VSSSP 581

BLAST of HG10011496 vs. NCBI nr
Match: KAG7015538.1 (65-kDa microtubule-associated protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1051.2 bits (2717), Expect = 3.4e-303
Identity = 558/605 (92.23%), Postives = 571/605 (94.38%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAVAD QNPLQGETTCGTLLQKLQEIWDEVGESD+ERDKMLLQIDQECLDVYKRKVEQAD
Sbjct: 1   MAVADIQNPLQGETTCGTLLQKLQEIWDEVGESDKERDKMLLQIDQECLDVYKRKVEQAD 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAK ELSSLLSALGEKSFSEFPEKA+GTIKEQLAAIAPALEQLWKQKE+
Sbjct: 61  KSRAKLLQSLSDAKLELSSLLSALGEKSFSEFPEKASGTIKEQLAAIAPALEQLWKQKEQ 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEI+GSLNLSENPVVDES LSVEKLDEYHCQLQELQKEKS+RLH
Sbjct: 121 RVKEFFDVQSEIQKICGEISGSLNLSENPVVDESHLSVEKLDEYHCQLQELQKEKSDRLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360
           EVEVERLDQLKAS+MKEIAFKKQSEL+EIFSHAHIE+DP+AAR+KIMALIDSGNVEPAEL
Sbjct: 301 EVEVERLDQLKASKMKEIAFKKQSELEEIFSHAHIEVDPDAARDKIMALIDSGNVEPAEL 360

Query: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSF 420
           LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR                 
Sbjct: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR----------------- 420

Query: 421 LHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480
                  DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD
Sbjct: 421 -------DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480

Query: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG 540
           GVPLLAMLDEYTMLRHEREEEKRR RD KKFHEQQ+TD E ITN RPSP RP+GTKKAAG
Sbjct: 481 GVPLLAMLDEYTMLRHEREEEKRRSRDQKKFHEQQSTDQETITNPRPSPVRPVGTKKAAG 540

Query: 541 PRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKEDSASHISGTEP 600
           PRANGAANGTPSSRRLSLNQNGSRSMNKDG+RDSMRPMTPVKYDAISKEDSASH SGTEP
Sbjct: 541 PRANGAANGTPSSRRLSLNQNGSRSMNKDGRRDSMRPMTPVKYDAISKEDSASHFSGTEP 581

Query: 601 VSSSP 606
           VSSSP
Sbjct: 601 VSSSP 581

BLAST of HG10011496 vs. NCBI nr
Match: KAA0055842.1 (65-kDa microtubule-associated protein 1-like isoform X2 [Cucumis melo var. makuwa] >TYK24066.1 65-kDa microtubule-associated protein 1-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1051.2 bits (2717), Expect = 3.4e-303
Identity = 561/605 (92.73%), Postives = 570/605 (94.21%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAVADTQ PLQGETTCGTLLQKLQEIWDEVGE+DEERDKMLLQIDQECLDVYKRKVE AD
Sbjct: 1   MAVADTQIPLQGETTCGTLLQKLQEIWDEVGETDEERDKMLLQIDQECLDVYKRKVELAD 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKA+GTIKEQLAAIAPALE+LWKQKE+
Sbjct: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKASGTIKEQLAAIAPALEKLWKQKEQ 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLS+EKLDEYHCQLQELQKEKSERLH
Sbjct: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSIEKLDEYHCQLQELQKEKSERLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360
           EVEVERLDQLKASRMKEIAFKKQSEL+EIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL
Sbjct: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360

Query: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSF 420
           LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR                 
Sbjct: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR----------------- 420

Query: 421 LHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480
                  DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTR WEEDRGISFIYD
Sbjct: 421 -------DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRTWEEDRGISFIYD 480

Query: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG 540
           GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFH+QQTTDPEIITNSRPSP R   TKKA G
Sbjct: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHDQQTTDPEIITNSRPSPVRTT-TKKAGG 540

Query: 541 PRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKEDSASHISGTEP 600
           PR NGAANGTPSSRRLSLNQNGSRSMNKD KRDSMRPMTPVKYDAISK+DS SHISGTEP
Sbjct: 541 PRVNGAANGTPSSRRLSLNQNGSRSMNKDSKRDSMRPMTPVKYDAISKDDSGSHISGTEP 580

Query: 601 VSSSP 606
           VS+SP
Sbjct: 601 VSASP 580

BLAST of HG10011496 vs. ExPASy Swiss-Prot
Match: Q9FLP0 (65-kDa microtubule-associated protein 1 OS=Arabidopsis thaliana OX=3702 GN=MAP65-1 PE=1 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 8.9e-230
Identity = 441/613 (71.94%), Postives = 509/613 (83.03%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAV DT++P  GE TCGTLL+KLQEIWDEVGESD+ERDK+LLQI+QECLDVYKRKVEQA 
Sbjct: 1   MAVTDTESPHLGEITCGTLLEKLQEIWDEVGESDDERDKLLLQIEQECLDVYKRKVEQAA 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRA+LLQ+LSDA  ELSSL  +LG+KS    P+K++GTIKEQLAAIAPALEQLW+QKE+
Sbjct: 61  KSRAELLQTLSDANAELSSLTMSLGDKSLVGIPDKSSGTIKEQLAAIAPALEQLWQQKEE 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RV+EF DVQS+IQKICG+IAG L+ +E P+VDES LS++KLD++  QLQELQKEKS+RL 
Sbjct: 121 RVREFSDVQSQIQKICGDIAGGLS-NEVPIVDESDLSLKKLDDFQSQLQELQKEKSDRLR 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLG+DF +TVTEVHPSL++ T VQSKSISN+TLS+LAK VL LK+D
Sbjct: 181 KVLEFVSTVHDLCAVLGLDFLSTVTEVHPSLDEDTSVQSKSISNETLSRLAKTVLTLKDD 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRL KLQELATQLIDLWNLMDTP EER LFDHVTCNIS+SVDEVTVPGALA DLIEQA
Sbjct: 241 KKQRLQKLQELATQLIDLWNLMDTPDEERELFDHVTCNISSSVDEVTVPGALARDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360
           EVEV+RLDQLKASRMKEIAFKKQSEL+EI++ AH+E++PE+ARE+IM+LIDSGNVEP EL
Sbjct: 301 EVEVDRLDQLKASRMKEIAFKKQSELEEIYARAHVEVNPESARERIMSLIDSGNVEPTEL 360

Query: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSF 420
           L DMD QI+KAKEE  SRK+ILD+VEKWM ACEEESWLEDYNR                 
Sbjct: 361 LADMDSQISKAKEEAFSRKDILDRVEKWMSACEEESWLEDYNR----------------- 420

Query: 421 LHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480
                  D NRY+ASRGAHLNLKRAEKARILV+KIPA+VDTL+AKTRAWEE+  +SF YD
Sbjct: 421 -------DQNRYSASRGAHLNLKRAEKARILVSKIPAMVDTLVAKTRAWEEEHSMSFAYD 480

Query: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG 540
           GVPLLAMLDEY MLR EREEEKRRLR+ KK  EQ   + E   ++RPSPARP+  KK  G
Sbjct: 481 GVPLLAMLDEYGMLRQEREEEKRRLREQKKVQEQPHVEQESAFSTRPSPARPVSAKKTVG 540

Query: 541 PRA-NGAANGTPSSRRLSL--NQNGSRSMNKD-GKRDSM-RPMTPVKYDAISKEDSASH- 600
           PRA NG ANGT  +RRLSL  NQNGSRS  K+ G+R+++ RP  P  Y AISKE++AS  
Sbjct: 541 PRANNGGANGT-HNRRLSLNANQNGSRSTAKEAGRRETLNRPAAPTNYVAISKEEAASSP 587

Query: 601 ISGT--EPVSSSP 606
           +SG     V +SP
Sbjct: 601 VSGAADHQVPASP 587

BLAST of HG10011496 vs. ExPASy Swiss-Prot
Match: Q8LEG3 (65-kDa microtubule-associated protein 2 OS=Arabidopsis thaliana OX=3702 GN=MAP65-2 PE=1 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 2.5e-216
Identity = 418/608 (68.75%), Postives = 485/608 (79.77%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAV + +NPL GE TCGTLLQKLQEIWDEVGESDEERDK+LLQI++ECL+VYK+KVE A 
Sbjct: 1   MAVTEAENPLLGEITCGTLLQKLQEIWDEVGESDEERDKLLLQIEEECLNVYKKKVELAA 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRA+LLQ+LSDA  ELS+L +ALGEKS+ + P+K +GTIKEQL+AIAPALEQLW+QKE+
Sbjct: 61  KSRAELLQTLSDATVELSNLTTALGEKSYIDIPDKTSGTIKEQLSAIAPALEQLWQQKEE 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RV+ F DVQS+IQKIC EIAG LN   + VVDE+ LS+++LD++  +LQELQKEKS+RL 
Sbjct: 121 RVRAFSDVQSQIQKICEEIAGGLNNGPH-VVDETDLSLKRLDDFQRKLQELQKEKSDRLQ 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVL +DF +TVTEVHPSL++A GVQ+KSISN+TL++LAK VL LKED
Sbjct: 181 KVLEFVSTVHDLCAVLRLDFLSTVTEVHPSLDEANGVQTKSISNETLARLAKTVLTLKED 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           K QRL KLQELATQL DLWNLMDT  EER LFDHVT NISASV EVT  GALALDLIEQA
Sbjct: 241 KMQRLKKLQELATQLTDLWNLMDTSDEERELFDHVTSNISASVHEVTASGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360
           EVEV+RLDQLK+SRMKEIAFKKQSEL+EI++ AHIEI PE  RE+IM+LID+GN EP EL
Sbjct: 301 EVEVDRLDQLKSSRMKEIAFKKQSELEEIYARAHIEIKPEVVRERIMSLIDAGNTEPTEL 360

Query: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSF 420
           L DMD QIAKAKEE  SRKEILD+VEKWM ACEEESWLEDYNR                 
Sbjct: 361 LADMDSQIAKAKEEAFSRKEILDRVEKWMSACEEESWLEDYNR----------------- 420

Query: 421 LHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480
                  D NRY+ASRGAHLNLKRAEKARILV+KI A+VDTLIAKTRAWEE+  +SF YD
Sbjct: 421 -------DQNRYSASRGAHLNLKRAEKARILVSKITAMVDTLIAKTRAWEEENSMSFEYD 480

Query: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG 540
           GVPLLAMLDEYTMLR ERE+EKRRL++ KK  EQ  TD E    S+PSPARP+  KK  G
Sbjct: 481 GVPLLAMLDEYTMLRQEREDEKRRLKEQKKQQEQPHTDQESAFGSKPSPARPVSAKKPVG 540

Query: 541 PRANGAANGTPSSRRLSL--NQNGSRSMNKDGKRDSMRPM-TPVKYDAISKEDSASHISG 600
            R NG        RRLS+  NQNGS+S     KRDS+  + +P    A +K+D+AS +S 
Sbjct: 541 TRVNGGGLNETPMRRLSMNSNQNGSKS-----KRDSLNKIASPSNIVANTKDDAASPVSR 578

Query: 601 TEPVSSSP 606
            +PV +SP
Sbjct: 601 ADPVMASP 578

BLAST of HG10011496 vs. ExPASy Swiss-Prot
Match: Q9FHM4 (65-kDa microtubule-associated protein 3 OS=Arabidopsis thaliana OX=3702 GN=MAP65-3 PE=1 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 2.8e-130
Identity = 262/575 (45.57%), Postives = 383/575 (66.61%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MA       LQ ETTCG+LL +LQ IWDEVGE++ +RD+MLL++++ECL+VY+RKV+QA+
Sbjct: 1   MASVQKDPILQVETTCGSLLFELQIIWDEVGETETDRDQMLLELERECLEVYRRKVDQAN 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFS-EFPEKAAGTIKEQLAAIAPALEQLWKQKE 120
           + RA+L Q+++DA+ +L+++ SA+GE+       +++ G++K++L  I P LE++ K+K 
Sbjct: 61  RCRAQLRQAIADAEAQLAAICSAMGERPVHIRQSDQSVGSLKQELGRILPELEEMQKRKV 120

Query: 121 KRVKEFFDVQSEIQKICGEIAGSLNL-SENPVVDESQLSVEKLDEYHCQLQELQKEKSER 180
           +R  +F  V  +I  I  +I G   L    P++DE+ LS+ KL+E HCQLQ LQKEK +R
Sbjct: 121 ERRNQFIVVMEQIDSITNDIKGQGELVHSEPLIDETNLSMRKLEELHCQLQVLQKEKIDR 180

Query: 181 LHKVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALK 240
           +  + + + T++  C+VLG+DF   V +V+P+L+D  G   +S+S+ T+ KL   V  L 
Sbjct: 181 VETIRKHLCTLYSHCSVLGMDFNEVVGQVNPTLSDPEG--PRSLSDHTIEKLGAAVQKLM 240

Query: 241 EDKKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIE 300
           E K QR+ +LQ+LAT +++LWNLMDTP EE+  + H+TCNI+AS  E+T   +L+ D I+
Sbjct: 241 EVKIQRMQRLQDLATTMLELWNLMDTPIEEQQEYQHITCNIAASEHEITEANSLSEDFIK 300

Query: 301 QAEVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPA 360
             E EV RLD++KAS+MKE+  KK+SEL+EI    H+    ++A ++ +  I+SG V+  
Sbjct: 301 YVEAEVVRLDEVKASKMKELVLKKRSELEEICRKTHLLPVSDSAIDQTIVAIESGIVDAT 360

Query: 361 ELLDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMED 420
            +L+ ++  I+K KEE LSRKEIL++VEKW+ AC+EESWLE+YNR               
Sbjct: 361 MVLEHLEQHISKIKEEALSRKEILERVEKWLSACDEESWLEEYNR--------------- 420

Query: 421 SFLHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFI 480
                    DDNRYNA RGAHL LKRAEKAR LV K+P +V+ L +KT  WE++ GI F+
Sbjct: 421 ---------DDNRYNAGRGAHLTLKRAEKARNLVTKLPGMVEALASKTIVWEQENGIEFL 480

Query: 481 YDGVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKA 540
           YDG+ LL+ML+EY +LR EREEE RR RD KK   Q   + E +  S+PSP++P+G KKA
Sbjct: 481 YDGIRLLSMLEEYNILRQEREEEHRRQRDQKKLQGQLIAEQEALYGSKPSPSKPLGGKKA 540

Query: 541 AGPRANGAANGTPSSRRLSLNQNGSRSMNKDGKRD 574
                 GA+N     RRLSL     ++   + K D
Sbjct: 541 PRMSTGGASN-----RRLSLGAAMHQTPKPNKKAD 544

BLAST of HG10011496 vs. ExPASy Swiss-Prot
Match: Q9SIS3 (65-kDa microtubule-associated protein 6 OS=Arabidopsis thaliana OX=3702 GN=MAP65-6 PE=1 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 3.5e-125
Identity = 264/612 (43.14%), Postives = 388/612 (63.40%), Query Frame = 0

Query: 14  TTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQADKSRAKLLQSLSDA 73
           TTC  LL++LQ+IW E+GE++ E+D+ML+++++ECL +Y+RKV++A  S+AKL QS++  
Sbjct: 16  TTCNNLLRELQKIWVEIGETETEKDRMLMELERECLQIYQRKVDEAANSKAKLHQSVASI 75

Query: 74  KHELSSLLSALGEKSFSE--FPEKAAGTIKEQLAAIAPALEQLWKQKEKRVKEFFDVQSE 133
           + E++SL++ALG  + +     +K + ++KE+LAA+ P +E+L  QKE+R+K+F D++++
Sbjct: 76  EAEVASLMAALGVLNINSPIKLDKGSKSLKEKLAAVTPLVEELRIQKEERMKQFSDIKAQ 135

Query: 134 IQKICGEIAG---SLNLSEN--PVVDESQLSVEKLDEYHCQLQELQKEKSERLHKVLEFV 193
           I+KI GEI+G    LN + N    ++E  L++  L+EY   L+ LQKEKS+RL+KVL +V
Sbjct: 136 IEKISGEISGYSDHLNKAMNISLTLEEQDLTLRNLNEYQTHLRTLQKEKSDRLNKVLGYV 195

Query: 194 STVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKEDKKQRLH 253
           + VH LC VLG+DF  TV+ VHPSL+     QS +IS+ TL  L  M+  LK ++K R  
Sbjct: 196 NEVHALCGVLGVDFSQTVSAVHPSLHRTDQEQSTNISDSTLEGLEHMIQKLKTERKSRFQ 255

Query: 254 KLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQAEVEVER 313
           KL+++   L +LWNLMDTP E+R+ F  VT  + +S   +T PG L+ + IEQ   EV+ 
Sbjct: 256 KLKDVVASLFELWNLMDTPQEDRTKFGKVTYVVRSSEANITEPGILSTETIEQVSTEVDS 315

Query: 314 LDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAELLDDMDD 373
           L +LKASRMKE+  K++SEL+++    HI+ D   + EK  ALIDSG V+P+ELL +++ 
Sbjct: 316 LSKLKASRMKELVMKRRSELEDLCRLTHIQPDTSTSAEKSTALIDSGLVDPSELLANIEM 375

Query: 374 QIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSFLHGCSY 433
           QI K K+E  SRK+I+D++++W+ ACEEE+WLE+YN                        
Sbjct: 376 QINKIKDEAQSRKDIMDRIDRWLSACEEENWLEEYN------------------------ 435

Query: 434 LDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLA 493
           LD+NRY+A RG H+NLKRAE+AR+ +NKIP +VDTLI KT  WEED   SF+YDGV L+ 
Sbjct: 436 LDENRYSAGRGGHVNLKRAERARVTINKIPGMVDTLIKKTLVWEEDMQKSFLYDGVRLVN 495

Query: 494 MLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG------ 553
           +L++Y + R ++EEEK+R RD KK  +   T  E I  S+PSP R    +K  G      
Sbjct: 496 ILEDYKLTRKQQEEEKKRYRDQKKRQDLLLTQRESIYGSKPSPRRSSSFRKPNGFNISNG 555

Query: 554 -------PRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKEDSAS 606
                  PR       TP       + +G    N   K       TP+ Y A+ KED+ S
Sbjct: 556 NGSVPPTPRRGSVGTTTPDVLLTPRSYSGHHRQNGYFKEVRRLSTTPLNYVAMQKEDTVS 602

BLAST of HG10011496 vs. ExPASy Swiss-Prot
Match: Q8L836 (65-kDa microtubule-associated protein 7 OS=Arabidopsis thaliana OX=3702 GN=MAP65-7 PE=2 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.3e-124
Identity = 270/611 (44.19%), Postives = 393/611 (64.32%), Query Frame = 0

Query: 14  TTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQADKSRAKLLQSLSDA 73
           TTC  LL++LQ+IW ++GESD E+D+ML+++++ECL++Y+RKV++A  S+A+L QSL   
Sbjct: 16  TTCNALLRELQKIWVDIGESDAEKDRMLMELEKECLEIYRRKVDEAANSKAQLHQSLVSI 75

Query: 74  KHELSSLLSALGEKSFSEFPEKA---AGTIKEQLAAIAPALEQLWKQKEKRVKEFFDVQS 133
           + E++SLL+ALG  + S  P KA   + ++KE+LAA+ P LE L  QK++R+K+F D+++
Sbjct: 76  EAEIASLLAALGVFN-SHSPMKAKEGSKSLKEKLAAVRPMLEDLRLQKDERMKQFVDIKA 135

Query: 134 EIQKICGEIAG-SLNLSENPV----VDESQLSVEKLDEYHCQLQELQKEKSERLHKVLEF 193
           +I+K+ GEI+G S  L++  V    +DE  L++ KL+EY   L+ LQKEKS+RL+KVL++
Sbjct: 136 QIEKMSGEISGYSDQLNKTMVGSLALDEQDLTLRKLNEYQTHLRSLQKEKSDRLNKVLDY 195

Query: 194 VSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKEDKKQRL 253
           V+ VH LC VLG+DF  TV+EVHPSL+     QS +IS+DTL  L  M+  LK ++  R 
Sbjct: 196 VNEVHTLCGVLGVDFGQTVSEVHPSLHRTDHEQSTNISDDTLDGLHHMIHKLKTERSVRF 255

Query: 254 HKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQAEVEVE 313
            KL+++A  L +LWNLMDT  EER+ F  V+  + +S  ++T P  L+ + IEQ   EV+
Sbjct: 256 QKLKDVAGSLFELWNLMDTSQEERTKFASVSYVVRSSESDITEPNILSSETIEQVSAEVD 315

Query: 314 RLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAELLDDMD 373
             ++LKASRMKE+  K+++EL+ +   AHIE D   + EK  ALIDSG V+P+ELL +++
Sbjct: 316 CFNKLKASRMKELVMKRRTELENLCRLAHIEADTSTSLEKSTALIDSGLVDPSELLTNIE 375

Query: 374 DQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSFLHGCS 433
             I K KEE  SRKEI+D++++W+ ACEEE+WLE+YN+                      
Sbjct: 376 LHINKIKEEAHSRKEIIDRIDRWLSACEEENWLEEYNQ---------------------- 435

Query: 434 YLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLL 493
             D+ RY+A RG H+NLK AE+ARI VNKIP++VD LI KT  WE++   SF+YDGV L+
Sbjct: 436 --DETRYSAGRGGHVNLKHAERARITVNKIPSMVDNLIKKTLLWEDETRKSFLYDGVRLV 495

Query: 494 AMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAGPRANG 553
           ++L++Y + R ++EEEKRR RD KK  +      E I  S+PSP R    +K  G   NG
Sbjct: 496 SILEDYKLTRKQQEEEKRRYRDQKKMQDLLIKRRESIYGSKPSPRRSNSVRKTNG--YNG 555

Query: 554 AANGTPSSRRLSLNQNGSRSMNKDGKRDSMR-----------PMTPVKYDAISKEDSAS- 603
            A+  P+ RR S     +  M       S R              P+ + AI KEDS S 
Sbjct: 556 DASVPPTPRRNSAGATNNDIMTTPRSYSSHRQNGYFKEVRRLSTAPLNFVAIPKEDSVST 599

BLAST of HG10011496 vs. ExPASy TrEMBL
Match: A0A0A0L2B7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G073820 PE=3 SV=1)

HSP 1 Score: 1055.4 bits (2728), Expect = 8.7e-305
Identity = 562/605 (92.89%), Postives = 571/605 (94.38%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAVADTQ PLQGETTCGTLLQKLQEIWDEVGE+DEERDKMLLQIDQECLDVYKRKVE AD
Sbjct: 1   MAVADTQIPLQGETTCGTLLQKLQEIWDEVGETDEERDKMLLQIDQECLDVYKRKVELAD 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKA+GTIKEQLAAIAPALE+LWKQKE+
Sbjct: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKASGTIKEQLAAIAPALEKLWKQKEQ 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKS+RLH
Sbjct: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSDRLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360
           EVEVERLDQLKASRMKEIAFKKQSEL+EIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL
Sbjct: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360

Query: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSF 420
           LDDMDDQI KAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR                 
Sbjct: 361 LDDMDDQITKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR----------------- 420

Query: 421 LHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480
                  DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD
Sbjct: 421 -------DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480

Query: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG 540
           GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFH+QQTTDPEIITNSRPSP R I TKKA G
Sbjct: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHDQQTTDPEIITNSRPSPVRTIATKKAGG 540

Query: 541 PRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKEDSASHISGTEP 600
            RANGAANGTPSSRRLSLNQNGSRSMNKD KRDSMRPMTPVKYDAISK+DS SHISGTEP
Sbjct: 541 ARANGAANGTPSSRRLSLNQNGSRSMNKDSKRDSMRPMTPVKYDAISKDDSGSHISGTEP 581

Query: 601 VSSSP 606
           VS+SP
Sbjct: 601 VSASP 581

BLAST of HG10011496 vs. ExPASy TrEMBL
Match: A0A5D3DKC5 (65-kDa microtubule-associated protein 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold943G00360 PE=3 SV=1)

HSP 1 Score: 1051.2 bits (2717), Expect = 1.6e-303
Identity = 561/605 (92.73%), Postives = 570/605 (94.21%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAVADTQ PLQGETTCGTLLQKLQEIWDEVGE+DEERDKMLLQIDQECLDVYKRKVE AD
Sbjct: 1   MAVADTQIPLQGETTCGTLLQKLQEIWDEVGETDEERDKMLLQIDQECLDVYKRKVELAD 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKA+GTIKEQLAAIAPALE+LWKQKE+
Sbjct: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKASGTIKEQLAAIAPALEKLWKQKEQ 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLS+EKLDEYHCQLQELQKEKSERLH
Sbjct: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSIEKLDEYHCQLQELQKEKSERLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360
           EVEVERLDQLKASRMKEIAFKKQSEL+EIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL
Sbjct: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360

Query: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSF 420
           LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR                 
Sbjct: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR----------------- 420

Query: 421 LHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480
                  DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTR WEEDRGISFIYD
Sbjct: 421 -------DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRTWEEDRGISFIYD 480

Query: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG 540
           GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFH+QQTTDPEIITNSRPSP R   TKKA G
Sbjct: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHDQQTTDPEIITNSRPSPVRTT-TKKAGG 540

Query: 541 PRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKEDSASHISGTEP 600
           PR NGAANGTPSSRRLSLNQNGSRSMNKD KRDSMRPMTPVKYDAISK+DS SHISGTEP
Sbjct: 541 PRVNGAANGTPSSRRLSLNQNGSRSMNKDSKRDSMRPMTPVKYDAISKDDSGSHISGTEP 580

Query: 601 VSSSP 606
           VS+SP
Sbjct: 601 VSASP 580

BLAST of HG10011496 vs. ExPASy TrEMBL
Match: A0A6J1EWG0 (65-kDa microtubule-associated protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111438596 PE=3 SV=1)

HSP 1 Score: 1049.3 bits (2712), Expect = 6.3e-303
Identity = 557/605 (92.07%), Postives = 570/605 (94.21%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAVAD QNPLQGETTCGTLLQKLQEIWDEVGESD+ERDKMLLQIDQECLDVYKRKVEQAD
Sbjct: 1   MAVADIQNPLQGETTCGTLLQKLQEIWDEVGESDKERDKMLLQIDQECLDVYKRKVEQAD 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAK ELSSLLSALGEKSFSEFPEKA+GTIKEQLAAIAPALEQLWKQKE+
Sbjct: 61  KSRAKLLQSLSDAKLELSSLLSALGEKSFSEFPEKASGTIKEQLAAIAPALEQLWKQKEQ 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEI+GSLNLSENPVVDES LSVEKLDEYHCQLQELQKEKS+RLH
Sbjct: 121 RVKEFFDVQSEIQKICGEISGSLNLSENPVVDESHLSVEKLDEYHCQLQELQKEKSDRLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360
           EVEVERLDQLKAS+MKEIAFKKQSEL+EIFSHAHIE+DP+AAR+KIMALIDSGNVEPAEL
Sbjct: 301 EVEVERLDQLKASKMKEIAFKKQSELEEIFSHAHIEVDPDAARDKIMALIDSGNVEPAEL 360

Query: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSF 420
           LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR                 
Sbjct: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR----------------- 420

Query: 421 LHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480
                  DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD
Sbjct: 421 -------DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480

Query: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG 540
           GVPLLAMLDEYTMLRHEREEEKRR RD KKFHEQQ+TD E I N RPSP RP+GTKKAAG
Sbjct: 481 GVPLLAMLDEYTMLRHEREEEKRRSRDQKKFHEQQSTDQETINNPRPSPVRPVGTKKAAG 540

Query: 541 PRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKEDSASHISGTEP 600
           PRANGAANGTPSSRRLSLNQNGSRSMNKDG+RDSMRPMTPVKYDAISKEDSASH SGTEP
Sbjct: 541 PRANGAANGTPSSRRLSLNQNGSRSMNKDGRRDSMRPMTPVKYDAISKEDSASHFSGTEP 581

Query: 601 VSSSP 606
           VSSSP
Sbjct: 601 VSSSP 581

BLAST of HG10011496 vs. ExPASy TrEMBL
Match: A0A6J1L8J6 (65-kDa microtubule-associated protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111500275 PE=3 SV=1)

HSP 1 Score: 1044.6 bits (2700), Expect = 1.5e-301
Identity = 554/605 (91.57%), Postives = 569/605 (94.05%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAVAD QNPLQGETTCGTLLQKLQEIWDEVGESD+ERDKMLLQIDQECLDVYKRKVEQAD
Sbjct: 1   MAVADIQNPLQGETTCGTLLQKLQEIWDEVGESDKERDKMLLQIDQECLDVYKRKVEQAD 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAK ELSSLLSALGEKSFSEFPEKA+GTIKEQLAAIAPALEQLWKQKE+
Sbjct: 61  KSRAKLLQSLSDAKLELSSLLSALGEKSFSEFPEKASGTIKEQLAAIAPALEQLWKQKEQ 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEI+GSLNL ENP+VDES LSVEKLDEYHCQLQELQKEKS+RLH
Sbjct: 121 RVKEFFDVQSEIQKICGEISGSLNLGENPLVDESHLSVEKLDEYHCQLQELQKEKSDRLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360
           EVEVERLDQLKAS+MKEIAFKKQSEL+EIFSHAHIE+DP+AAR+KI+ALIDSGNVEPAEL
Sbjct: 301 EVEVERLDQLKASKMKEIAFKKQSELEEIFSHAHIEVDPDAARDKILALIDSGNVEPAEL 360

Query: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSF 420
           LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR                 
Sbjct: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR----------------- 420

Query: 421 LHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480
                  DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRG+SFIYD
Sbjct: 421 -------DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGLSFIYD 480

Query: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG 540
           GVPLLAMLDEYTMLRHEREEEKRR RD KKFHEQQ+TD E ITN RPSP RPIGTKKAAG
Sbjct: 481 GVPLLAMLDEYTMLRHEREEEKRRSRDQKKFHEQQSTDQEAITNPRPSPVRPIGTKKAAG 540

Query: 541 PRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKEDSASHISGTEP 600
           PRANGAANGTPSSRRLSLNQNGSRSMNKDG+RDSMRPMTPVKYDAISKEDSA H SGTEP
Sbjct: 541 PRANGAANGTPSSRRLSLNQNGSRSMNKDGRRDSMRPMTPVKYDAISKEDSALHFSGTEP 581

Query: 601 VSSSP 606
           VSSSP
Sbjct: 601 VSSSP 581

BLAST of HG10011496 vs. ExPASy TrEMBL
Match: A0A6J1KSV7 (65-kDa microtubule-associated protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111498370 PE=3 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 1.0e-300
Identity = 555/607 (91.43%), Postives = 567/607 (93.41%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAV DT+N LQGETTCGTLLQKLQEIWDEVGESDE+RDKMLLQIDQECLDVYKRKVEQAD
Sbjct: 1   MAVEDTKNSLQGETTCGTLLQKLQEIWDEVGESDEDRDKMLLQIDQECLDVYKRKVEQAD 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRAKLLQSLSDAKHELS+LLSALGEKSFSEFPE A+GTIKEQLAAIAPALEQLWKQKEK
Sbjct: 61  KSRAKLLQSLSDAKHELSNLLSALGEKSFSEFPESASGTIKEQLAAIAPALEQLWKQKEK 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RVKEFFDVQSEIQKICGEI GSLN+SENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH
Sbjct: 121 RVKEFFDVQSEIQKICGEITGSLNISENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLGIDFF TVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED
Sbjct: 181 KVLEFVSTVHDLCAVLGIDFFGTVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA
Sbjct: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360
           EVEVERLDQLKASRMKEIAFKKQSEL+EIF+HAHIEI PEAAREKIM LIDSGNVEP+EL
Sbjct: 301 EVEVERLDQLKASRMKEIAFKKQSELEEIFAHAHIEIVPEAAREKIMTLIDSGNVEPSEL 360

Query: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSF 420
           LDDMD++I KAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR                 
Sbjct: 361 LDDMDNKIVKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNR----------------- 420

Query: 421 LHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480
                  DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD
Sbjct: 421 -------DDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480

Query: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG 540
           GVPLLAMLDEYTMLRHEREEEKRR RDHKKFHEQQ+TD E ITNSRPSPARPIGTKKAAG
Sbjct: 481 GVPLLAMLDEYTMLRHEREEEKRRFRDHKKFHEQQSTDQESITNSRPSPARPIGTKKAAG 540

Query: 541 PRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKED--SASHISGT 600
           PRANGA NGTPSSRRLSLNQNGSRSMNKDGKRD MRPMTPVKYDAISKED  S SH+SGT
Sbjct: 541 PRANGAVNGTPSSRRLSLNQNGSRSMNKDGKRDGMRPMTPVKYDAISKEDSGSGSHVSGT 583

Query: 601 EPVSSSP 606
           EPVSSSP
Sbjct: 601 EPVSSSP 583

BLAST of HG10011496 vs. TAIR 10
Match: AT5G55230.1 (microtubule-associated proteins 65-1 )

HSP 1 Score: 797.7 bits (2059), Expect = 6.3e-231
Identity = 441/613 (71.94%), Postives = 509/613 (83.03%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAV DT++P  GE TCGTLL+KLQEIWDEVGESD+ERDK+LLQI+QECLDVYKRKVEQA 
Sbjct: 1   MAVTDTESPHLGEITCGTLLEKLQEIWDEVGESDDERDKLLLQIEQECLDVYKRKVEQAA 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRA+LLQ+LSDA  ELSSL  +LG+KS    P+K++GTIKEQLAAIAPALEQLW+QKE+
Sbjct: 61  KSRAELLQTLSDANAELSSLTMSLGDKSLVGIPDKSSGTIKEQLAAIAPALEQLWQQKEE 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RV+EF DVQS+IQKICG+IAG L+ +E P+VDES LS++KLD++  QLQELQKEKS+RL 
Sbjct: 121 RVREFSDVQSQIQKICGDIAGGLS-NEVPIVDESDLSLKKLDDFQSQLQELQKEKSDRLR 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLG+DF +TVTEVHPSL++ T VQSKSISN+TLS+LAK VL LK+D
Sbjct: 181 KVLEFVSTVHDLCAVLGLDFLSTVTEVHPSLDEDTSVQSKSISNETLSRLAKTVLTLKDD 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           KKQRL KLQELATQLIDLWNLMDTP EER LFDHVTCNIS+SVDEVTVPGALA DLIEQA
Sbjct: 241 KKQRLQKLQELATQLIDLWNLMDTPDEERELFDHVTCNISSSVDEVTVPGALARDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360
           EVEV+RLDQLKASRMKEIAFKKQSEL+EI++ AH+E++PE+ARE+IM+LIDSGNVEP EL
Sbjct: 301 EVEVDRLDQLKASRMKEIAFKKQSELEEIYARAHVEVNPESARERIMSLIDSGNVEPTEL 360

Query: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSF 420
           L DMD QI+KAKEE  SRK+ILD+VEKWM ACEEESWLEDYNR                 
Sbjct: 361 LADMDSQISKAKEEAFSRKDILDRVEKWMSACEEESWLEDYNR----------------- 420

Query: 421 LHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480
                  D NRY+ASRGAHLNLKRAEKARILV+KIPA+VDTL+AKTRAWEE+  +SF YD
Sbjct: 421 -------DQNRYSASRGAHLNLKRAEKARILVSKIPAMVDTLVAKTRAWEEEHSMSFAYD 480

Query: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG 540
           GVPLLAMLDEY MLR EREEEKRRLR+ KK  EQ   + E   ++RPSPARP+  KK  G
Sbjct: 481 GVPLLAMLDEYGMLRQEREEEKRRLREQKKVQEQPHVEQESAFSTRPSPARPVSAKKTVG 540

Query: 541 PRA-NGAANGTPSSRRLSL--NQNGSRSMNKD-GKRDSM-RPMTPVKYDAISKEDSASH- 600
           PRA NG ANGT  +RRLSL  NQNGSRS  K+ G+R+++ RP  P  Y AISKE++AS  
Sbjct: 541 PRANNGGANGT-HNRRLSLNANQNGSRSTAKEAGRRETLNRPAAPTNYVAISKEEAASSP 587

Query: 601 ISGT--EPVSSSP 606
           +SG     V +SP
Sbjct: 601 VSGAADHQVPASP 587

BLAST of HG10011496 vs. TAIR 10
Match: AT5G55230.2 (microtubule-associated proteins 65-1 )

HSP 1 Score: 782.3 bits (2019), Expect = 2.7e-226
Identity = 441/642 (68.69%), Postives = 509/642 (79.28%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAV DT++P  GE TCGTLL+KLQEIWDEVGESD+ERDK+LLQI+QECLDVYKRKVEQA 
Sbjct: 1   MAVTDTESPHLGEITCGTLLEKLQEIWDEVGESDDERDKLLLQIEQECLDVYKRKVEQAA 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRA+LLQ+LSDA  ELSSL  +LG+KS    P+K++GTIKEQLAAIAPALEQLW+QKE+
Sbjct: 61  KSRAELLQTLSDANAELSSLTMSLGDKSLVGIPDKSSGTIKEQLAAIAPALEQLWQQKEE 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RV+EF DVQS+IQKICG+IAG L+ +E P+VDES LS++KLD++  QLQELQKEKS+RL 
Sbjct: 121 RVREFSDVQSQIQKICGDIAGGLS-NEVPIVDESDLSLKKLDDFQSQLQELQKEKSDRLR 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVLG+DF +TVTEVHPSL++ T VQSKSISN+TLS+LAK VL LK+D
Sbjct: 181 KVLEFVSTVHDLCAVLGLDFLSTVTEVHPSLDEDTSVQSKSISNETLSRLAKTVLTLKDD 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQ- 300
           KKQRL KLQELATQLIDLWNLMDTP EER LFDHVTCNIS+SVDEVTVPGALA DLIEQ 
Sbjct: 241 KKQRLQKLQELATQLIDLWNLMDTPDEERELFDHVTCNISSSVDEVTVPGALARDLIEQV 300

Query: 301 ----------------------------AEVEVERLDQLKASRMKEIAFKKQSELDEIFS 360
                                       AEVEV+RLDQLKASRMKEIAFKKQSEL+EI++
Sbjct: 301 IYIALINLPMSSLRNQLLLANIHVKFVKAEVEVDRLDQLKASRMKEIAFKKQSELEEIYA 360

Query: 361 HAHIEIDPEAAREKIMALIDSGNVEPAELLDDMDDQIAKAKEEVLSRKEILDKVEKWMLA 420
            AH+E++PE+ARE+IM+LIDSGNVEP ELL DMD QI+KAKEE  SRK+ILD+VEKWM A
Sbjct: 361 RAHVEVNPESARERIMSLIDSGNVEPTELLADMDSQISKAKEEAFSRKDILDRVEKWMSA 420

Query: 421 CEEESWLEDYNRISMPGRMLLKAAMEDSFLHGCSYLDDNRYNASRGAHLNLKRAEKARIL 480
           CEEESWLEDYNR                        D NRY+ASRGAHLNLKRAEKARIL
Sbjct: 421 CEEESWLEDYNR------------------------DQNRYSASRGAHLNLKRAEKARIL 480

Query: 481 VNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLAMLDEYTMLRHEREEEKRRLRDHKKF 540
           V+KIPA+VDTL+AKTRAWEE+  +SF YDGVPLLAMLDEY MLR EREEEKRRLR+ KK 
Sbjct: 481 VSKIPAMVDTLVAKTRAWEEEHSMSFAYDGVPLLAMLDEYGMLRQEREEEKRRLREQKKV 540

Query: 541 HEQQTTDPEIITNSRPSPARPIGTKKAAGPRA-NGAANGTPSSRRLSL--NQNGSRSMNK 600
            EQ   + E   ++RPSPARP+  KK  GPRA NG ANGT  +RRLSL  NQNGSRS  K
Sbjct: 541 QEQPHVEQESAFSTRPSPARPVSAKKTVGPRANNGGANGT-HNRRLSLNANQNGSRSTAK 600

Query: 601 D-GKRDSM-RPMTPVKYDAISKEDSASH-ISGT--EPVSSSP 606
           + G+R+++ RP  P  Y AISKE++AS  +SG     V +SP
Sbjct: 601 EAGRRETLNRPAAPTNYVAISKEEAASSPVSGAADHQVPASP 616

BLAST of HG10011496 vs. TAIR 10
Match: AT4G26760.1 (microtubule-associated protein 65-2 )

HSP 1 Score: 753.1 bits (1943), Expect = 1.8e-217
Identity = 418/608 (68.75%), Postives = 485/608 (79.77%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MAV + +NPL GE TCGTLLQKLQEIWDEVGESDEERDK+LLQI++ECL+VYK+KVE A 
Sbjct: 1   MAVTEAENPLLGEITCGTLLQKLQEIWDEVGESDEERDKLLLQIEEECLNVYKKKVELAA 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFSEFPEKAAGTIKEQLAAIAPALEQLWKQKEK 120
           KSRA+LLQ+LSDA  ELS+L +ALGEKS+ + P+K +GTIKEQL+AIAPALEQLW+QKE+
Sbjct: 61  KSRAELLQTLSDATVELSNLTTALGEKSYIDIPDKTSGTIKEQLSAIAPALEQLWQQKEE 120

Query: 121 RVKEFFDVQSEIQKICGEIAGSLNLSENPVVDESQLSVEKLDEYHCQLQELQKEKSERLH 180
           RV+ F DVQS+IQKIC EIAG LN   + VVDE+ LS+++LD++  +LQELQKEKS+RL 
Sbjct: 121 RVRAFSDVQSQIQKICEEIAGGLNNGPH-VVDETDLSLKRLDDFQRKLQELQKEKSDRLQ 180

Query: 181 KVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKED 240
           KVLEFVSTVHDLCAVL +DF +TVTEVHPSL++A GVQ+KSISN+TL++LAK VL LKED
Sbjct: 181 KVLEFVSTVHDLCAVLRLDFLSTVTEVHPSLDEANGVQTKSISNETLARLAKTVLTLKED 240

Query: 241 KKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQA 300
           K QRL KLQELATQL DLWNLMDT  EER LFDHVT NISASV EVT  GALALDLIEQA
Sbjct: 241 KMQRLKKLQELATQLTDLWNLMDTSDEERELFDHVTSNISASVHEVTASGALALDLIEQA 300

Query: 301 EVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAEL 360
           EVEV+RLDQLK+SRMKEIAFKKQSEL+EI++ AHIEI PE  RE+IM+LID+GN EP EL
Sbjct: 301 EVEVDRLDQLKSSRMKEIAFKKQSELEEIYARAHIEIKPEVVRERIMSLIDAGNTEPTEL 360

Query: 361 LDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSF 420
           L DMD QIAKAKEE  SRKEILD+VEKWM ACEEESWLEDYNR                 
Sbjct: 361 LADMDSQIAKAKEEAFSRKEILDRVEKWMSACEEESWLEDYNR----------------- 420

Query: 421 LHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYD 480
                  D NRY+ASRGAHLNLKRAEKARILV+KI A+VDTLIAKTRAWEE+  +SF YD
Sbjct: 421 -------DQNRYSASRGAHLNLKRAEKARILVSKITAMVDTLIAKTRAWEEENSMSFEYD 480

Query: 481 GVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG 540
           GVPLLAMLDEYTMLR ERE+EKRRL++ KK  EQ  TD E    S+PSPARP+  KK  G
Sbjct: 481 GVPLLAMLDEYTMLRQEREDEKRRLKEQKKQQEQPHTDQESAFGSKPSPARPVSAKKPVG 540

Query: 541 PRANGAANGTPSSRRLSL--NQNGSRSMNKDGKRDSMRPM-TPVKYDAISKEDSASHISG 600
            R NG        RRLS+  NQNGS+S     KRDS+  + +P    A +K+D+AS +S 
Sbjct: 541 TRVNGGGLNETPMRRLSMNSNQNGSKS-----KRDSLNKIASPSNIVANTKDDAASPVSR 578

Query: 601 TEPVSSSP 606
            +PV +SP
Sbjct: 601 ADPVMASP 578

BLAST of HG10011496 vs. TAIR 10
Match: AT5G51600.1 (Microtubule associated protein (MAP65/ASE1) family protein )

HSP 1 Score: 467.2 bits (1201), Expect = 2.0e-131
Identity = 262/575 (45.57%), Postives = 383/575 (66.61%), Query Frame = 0

Query: 1   MAVADTQNPLQGETTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQAD 60
           MA       LQ ETTCG+LL +LQ IWDEVGE++ +RD+MLL++++ECL+VY+RKV+QA+
Sbjct: 1   MASVQKDPILQVETTCGSLLFELQIIWDEVGETETDRDQMLLELERECLEVYRRKVDQAN 60

Query: 61  KSRAKLLQSLSDAKHELSSLLSALGEKSFS-EFPEKAAGTIKEQLAAIAPALEQLWKQKE 120
           + RA+L Q+++DA+ +L+++ SA+GE+       +++ G++K++L  I P LE++ K+K 
Sbjct: 61  RCRAQLRQAIADAEAQLAAICSAMGERPVHIRQSDQSVGSLKQELGRILPELEEMQKRKV 120

Query: 121 KRVKEFFDVQSEIQKICGEIAGSLNL-SENPVVDESQLSVEKLDEYHCQLQELQKEKSER 180
           +R  +F  V  +I  I  +I G   L    P++DE+ LS+ KL+E HCQLQ LQKEK +R
Sbjct: 121 ERRNQFIVVMEQIDSITNDIKGQGELVHSEPLIDETNLSMRKLEELHCQLQVLQKEKIDR 180

Query: 181 LHKVLEFVSTVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALK 240
           +  + + + T++  C+VLG+DF   V +V+P+L+D  G   +S+S+ T+ KL   V  L 
Sbjct: 181 VETIRKHLCTLYSHCSVLGMDFNEVVGQVNPTLSDPEG--PRSLSDHTIEKLGAAVQKLM 240

Query: 241 EDKKQRLHKLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIE 300
           E K QR+ +LQ+LAT +++LWNLMDTP EE+  + H+TCNI+AS  E+T   +L+ D I+
Sbjct: 241 EVKIQRMQRLQDLATTMLELWNLMDTPIEEQQEYQHITCNIAASEHEITEANSLSEDFIK 300

Query: 301 QAEVEVERLDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPA 360
             E EV RLD++KAS+MKE+  KK+SEL+EI    H+    ++A ++ +  I+SG V+  
Sbjct: 301 YVEAEVVRLDEVKASKMKELVLKKRSELEEICRKTHLLPVSDSAIDQTIVAIESGIVDAT 360

Query: 361 ELLDDMDDQIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMED 420
            +L+ ++  I+K KEE LSRKEIL++VEKW+ AC+EESWLE+YNR               
Sbjct: 361 MVLEHLEQHISKIKEEALSRKEILERVEKWLSACDEESWLEEYNR--------------- 420

Query: 421 SFLHGCSYLDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFI 480
                    DDNRYNA RGAHL LKRAEKAR LV K+P +V+ L +KT  WE++ GI F+
Sbjct: 421 ---------DDNRYNAGRGAHLTLKRAEKARNLVTKLPGMVEALASKTIVWEQENGIEFL 480

Query: 481 YDGVPLLAMLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKA 540
           YDG+ LL+ML+EY +LR EREEE RR RD KK   Q   + E +  S+PSP++P+G KKA
Sbjct: 481 YDGIRLLSMLEEYNILRQEREEEHRRQRDQKKLQGQLIAEQEALYGSKPSPSKPLGGKKA 540

Query: 541 AGPRANGAANGTPSSRRLSLNQNGSRSMNKDGKRD 574
                 GA+N     RRLSL     ++   + K D
Sbjct: 541 PRMSTGGASN-----RRLSLGAAMHQTPKPNKKAD 544

BLAST of HG10011496 vs. TAIR 10
Match: AT2G01910.1 (Microtubule associated protein (MAP65/ASE1) family protein )

HSP 1 Score: 450.3 bits (1157), Expect = 2.5e-126
Identity = 264/612 (43.14%), Postives = 388/612 (63.40%), Query Frame = 0

Query: 14  TTCGTLLQKLQEIWDEVGESDEERDKMLLQIDQECLDVYKRKVEQADKSRAKLLQSLSDA 73
           TTC  LL++LQ+IW E+GE++ E+D+ML+++++ECL +Y+RKV++A  S+AKL QS++  
Sbjct: 16  TTCNNLLRELQKIWVEIGETETEKDRMLMELERECLQIYQRKVDEAANSKAKLHQSVASI 75

Query: 74  KHELSSLLSALGEKSFSE--FPEKAAGTIKEQLAAIAPALEQLWKQKEKRVKEFFDVQSE 133
           + E++SL++ALG  + +     +K + ++KE+LAA+ P +E+L  QKE+R+K+F D++++
Sbjct: 76  EAEVASLMAALGVLNINSPIKLDKGSKSLKEKLAAVTPLVEELRIQKEERMKQFSDIKAQ 135

Query: 134 IQKICGEIAG---SLNLSEN--PVVDESQLSVEKLDEYHCQLQELQKEKSERLHKVLEFV 193
           I+KI GEI+G    LN + N    ++E  L++  L+EY   L+ LQKEKS+RL+KVL +V
Sbjct: 136 IEKISGEISGYSDHLNKAMNISLTLEEQDLTLRNLNEYQTHLRTLQKEKSDRLNKVLGYV 195

Query: 194 STVHDLCAVLGIDFFATVTEVHPSLNDATGVQSKSISNDTLSKLAKMVLALKEDKKQRLH 253
           + VH LC VLG+DF  TV+ VHPSL+     QS +IS+ TL  L  M+  LK ++K R  
Sbjct: 196 NEVHALCGVLGVDFSQTVSAVHPSLHRTDQEQSTNISDSTLEGLEHMIQKLKTERKSRFQ 255

Query: 254 KLQELATQLIDLWNLMDTPSEERSLFDHVTCNISASVDEVTVPGALALDLIEQAEVEVER 313
           KL+++   L +LWNLMDTP E+R+ F  VT  + +S   +T PG L+ + IEQ   EV+ 
Sbjct: 256 KLKDVVASLFELWNLMDTPQEDRTKFGKVTYVVRSSEANITEPGILSTETIEQVSTEVDS 315

Query: 314 LDQLKASRMKEIAFKKQSELDEIFSHAHIEIDPEAAREKIMALIDSGNVEPAELLDDMDD 373
           L +LKASRMKE+  K++SEL+++    HI+ D   + EK  ALIDSG V+P+ELL +++ 
Sbjct: 316 LSKLKASRMKELVMKRRSELEDLCRLTHIQPDTSTSAEKSTALIDSGLVDPSELLANIEM 375

Query: 374 QIAKAKEEVLSRKEILDKVEKWMLACEEESWLEDYNRISMPGRMLLKAAMEDSFLHGCSY 433
           QI K K+E  SRK+I+D++++W+ ACEEE+WLE+YN                        
Sbjct: 376 QINKIKDEAQSRKDIMDRIDRWLSACEEENWLEEYN------------------------ 435

Query: 434 LDDNRYNASRGAHLNLKRAEKARILVNKIPALVDTLIAKTRAWEEDRGISFIYDGVPLLA 493
           LD+NRY+A RG H+NLKRAE+AR+ +NKIP +VDTLI KT  WEED   SF+YDGV L+ 
Sbjct: 436 LDENRYSAGRGGHVNLKRAERARVTINKIPGMVDTLIKKTLVWEEDMQKSFLYDGVRLVN 495

Query: 494 MLDEYTMLRHEREEEKRRLRDHKKFHEQQTTDPEIITNSRPSPARPIGTKKAAG------ 553
           +L++Y + R ++EEEK+R RD KK  +   T  E I  S+PSP R    +K  G      
Sbjct: 496 ILEDYKLTRKQQEEEKKRYRDQKKRQDLLLTQRESIYGSKPSPRRSSSFRKPNGFNISNG 555

Query: 554 -------PRANGAANGTPSSRRLSLNQNGSRSMNKDGKRDSMRPMTPVKYDAISKEDSAS 606
                  PR       TP       + +G    N   K       TP+ Y A+ KED+ S
Sbjct: 556 NGSVPPTPRRGSVGTTTPDVLLTPRSYSGHHRQNGYFKEVRRLSTTPLNYVAMQKEDTVS 602

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038905625.10.0e+0094.8865-kDa microtubule-associated protein 1-like [Benincasa hispida][more]
XP_011650516.11.8e-30492.8965-kDa microtubule-associated protein 1 [Cucumis sativus] >KGN56090.1 hypothetic... [more]
KAG7028419.16.9e-30492.0765-kDa microtubule-associated protein 1 [Cucurbita argyrosperma subsp. argyrospe... [more]
KAG7015538.13.4e-30392.2365-kDa microtubule-associated protein 1 [Cucurbita argyrosperma subsp. argyrospe... [more]
KAA0055842.13.4e-30392.7365-kDa microtubule-associated protein 1-like isoform X2 [Cucumis melo var. makuw... [more]
Match NameE-valueIdentityDescription
Q9FLP08.9e-23071.9465-kDa microtubule-associated protein 1 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
Q8LEG32.5e-21668.7565-kDa microtubule-associated protein 2 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
Q9FHM42.8e-13045.5765-kDa microtubule-associated protein 3 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
Q9SIS33.5e-12543.1465-kDa microtubule-associated protein 6 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
Q8L8361.3e-12444.1965-kDa microtubule-associated protein 7 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
Match NameE-valueIdentityDescription
A0A0A0L2B78.7e-30592.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G073820 PE=3 SV=1[more]
A0A5D3DKC51.6e-30392.7365-kDa microtubule-associated protein 1-like isoform X2 OS=Cucumis melo var. mak... [more]
A0A6J1EWG06.3e-30392.0765-kDa microtubule-associated protein 1-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1L8J61.5e-30191.5765-kDa microtubule-associated protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1KSV71.0e-30091.4365-kDa microtubule-associated protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT5G55230.16.3e-23171.94microtubule-associated proteins 65-1 [more]
AT5G55230.22.7e-22668.69microtubule-associated proteins 65-1 [more]
AT4G26760.11.8e-21768.75microtubule-associated protein 65-2 [more]
AT5G51600.12.0e-13145.57Microtubule associated protein (MAP65/ASE1) family protein [more]
AT2G01910.12.5e-12643.14Microtubule associated protein (MAP65/ASE1) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 56..76
NoneNo IPR availableCOILSCoilCoilcoord: 495..515
NoneNo IPR availableCOILSCoilCoilcoord: 238..258
NoneNo IPR availableCOILSCoilCoilcoord: 297..317
NoneNo IPR availablePFAMPF03999MAP65_ASE1coord: 428..569
e-value: 2.5E-23
score: 82.6
coord: 52..403
e-value: 1.0E-50
score: 173.1
NoneNo IPR availableGENE3D1.20.58.1520coord: 367..525
e-value: 2.8E-33
score: 116.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 591..605
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 502..605
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 502..516
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 546..565
NoneNo IPR availablePANTHERPTHR19321:SF3765-KDA MICROTUBULE-ASSOCIATED PROTEIN 1coord: 5..403
coord: 412..605
IPR007145Microtubule-associated protein, MAP65/Ase1/PRC1PANTHERPTHR19321PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATEDcoord: 412..605
coord: 5..403

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10011496.1HG10011496.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000911 cytokinesis by cell plate formation
biological_process GO:0000226 microtubule cytoskeleton organization
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005874 microtubule
cellular_component GO:0005819 spindle
molecular_function GO:0008017 microtubule binding