HG10010826 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10010826 vs. NCBI nr
Match: XP_038876325.1 (LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 1043.1 bits (2696), Expect = 8.6e-301
Identity = 537/557 (96.41%), Postives = 545/557 (97.85%), Query Frame = 0

Query: 1   MLNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60
           M+NQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW
Sbjct: 44  MVNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 103

Query: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNT 120
           CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLL RIVSLPYSMVY+LCLMAGCSICWFNT
Sbjct: 104 CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIVSLPYSMVYLLCLMAGCSICWFNT 163

Query: 121 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDASLYLFLNALVPLIISV 180
           VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLI+NAIDPNDASLYLFLNALVPLIISV
Sbjct: 164 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAIDPNDASLYLFLNALVPLIISV 223

Query: 181 VALLPILHQPPVQPSSADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQILLAG 240
           VA LPILHQPPVQPSSADAIRHDSLIFICLYI A+ITGLYLI+FNSMPSNKYGSQILLAG
Sbjct: 224 VAFLPILHQPPVQPSSADAIRHDSLIFICLYITAIITGLYLISFNSMPSNKYGSQILLAG 283

Query: 241 AFALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNSMKV 300
           AFALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERN M V
Sbjct: 284 AFALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNGMTV 343

Query: 301 TTPFDSKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYVAYFCGGTIGLVYSNN 360
             PFDS EKESISRKVMEKENLVVLEEEHSA MLMRRLDFWLYYVAYFCGGTIGLVYSNN
Sbjct: 344 IVPFDSNEKESISRKVMEKENLVVLEEEHSAKMLMRRLDFWLYYVAYFCGGTIGLVYSNN 403

Query: 361 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISATPDFMREKVHFARTGWLAIALVPTPIA 420
           LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISA PDFMREKVHFARTGWLAIALVPTPIA
Sbjct: 404 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREKVHFARTGWLAIALVPTPIA 463

Query: 421 FILLAASGSKVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 480
           FILLAAS SK+ALQAGTGLIGL SGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF
Sbjct: 464 FILLAASDSKIALQAGTGLIGLCSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 523

Query: 481 LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRT 540
           LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRT
Sbjct: 524 LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRT 583

Query: 541 RPAYDRHYESNLSKMQP 558
           +PAYDRHYESN+SKMQP
Sbjct: 584 KPAYDRHYESNVSKMQP 600

BLAST of HG10010826 vs. NCBI nr
Match: XP_008460080.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo])

HSP 1 Score: 1020.4 bits (2637), Expect = 5.9e-294
Identity = 524/558 (93.91%), Postives = 539/558 (96.59%), Query Frame = 0

Query: 1   MLNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60
           M+NQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW
Sbjct: 22  MMNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 81

Query: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNT 120
           CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLL RI+SLPYS+VY+LCLMAGCSICWFNT
Sbjct: 82  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIISLPYSVVYLLCLMAGCSICWFNT 141

Query: 121 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDASLYLFLNALVPLIISV 180
           VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLI+NAIDP DASLYLFLNALVPLIISV
Sbjct: 142 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAIDPGDASLYLFLNALVPLIISV 201

Query: 181 VALLPILHQPPVQPSSADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQILLAG 240
           VALLP+LHQP VQPSSADAIRHDSLIFICLYI AVITGLYLITFNSMPSNKYGSQILLAG
Sbjct: 202 VALLPMLHQPQVQPSSADAIRHDSLIFICLYITAVITGLYLITFNSMPSNKYGSQILLAG 261

Query: 241 AFALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNSMKV 300
           AFALLLVPLCLPGILST RWLVRIISTSL+CLIHSRF+L DHELHQEL  +G+ERNSM  
Sbjct: 262 AFALLLVPLCLPGILSTHRWLVRIISTSLNCLIHSRFSLADHELHQELFTIGSERNSMMD 321

Query: 301 TTPFDSKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYVAYFCGGTIGLVYSNN 360
             PF+SKEKESISRKVMEKENLVVLEEEHSA MLMR+LDFWLYY AYFCGGTIGLVYSNN
Sbjct: 322 IIPFESKEKESISRKVMEKENLVVLEEEHSAKMLMRQLDFWLYYAAYFCGGTIGLVYSNN 381

Query: 361 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISATPDFMREKVHFARTGWLAIALVPTPIA 420
           LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISA PDFMREK HFARTGWLAIALVPTPIA
Sbjct: 382 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREKGHFARTGWLAIALVPTPIA 441

Query: 421 FILLAASGSKVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 480
           F+LLAASGSK+ALQAGT LIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF
Sbjct: 442 FVLLAASGSKIALQAGTSLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 501

Query: 481 LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRT 540
           LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYL TFVWWACISIFGLASSFLLFRRT
Sbjct: 502 LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLMTFVWWACISIFGLASSFLLFRRT 561

Query: 541 RPAYDRHYESNLSKMQPF 559
           + AYDRHYESNLSKMQPF
Sbjct: 562 KSAYDRHYESNLSKMQPF 579

BLAST of HG10010826 vs. NCBI nr
Match: KAA0039979.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK24523.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.7e-293
Identity = 523/558 (93.73%), Postives = 540/558 (96.77%), Query Frame = 0

Query: 1   MLNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60
           M+NQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW
Sbjct: 1   MMNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60

Query: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNT 120
           CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLL RI+SLPYS+VY+LCLMAGCSICWFNT
Sbjct: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIISLPYSVVYLLCLMAGCSICWFNT 120

Query: 121 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDASLYLFLNALVPLIISV 180
           VCYVSCIQNFPA+RALALSLIVSFNGVSAALYTLI+NAIDP DASLYLFLNALVPLIISV
Sbjct: 121 VCYVSCIQNFPAHRALALSLIVSFNGVSAALYTLIANAIDPGDASLYLFLNALVPLIISV 180

Query: 181 VALLPILHQPPVQPSSADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQILLAG 240
           VALLP+LHQP VQPSSADAIRHDSLIFICLYI AVITGLYLITFNSMPSNKYGSQILLAG
Sbjct: 181 VALLPMLHQPQVQPSSADAIRHDSLIFICLYITAVITGLYLITFNSMPSNKYGSQILLAG 240

Query: 241 AFALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNSMKV 300
           AFALLLVPLCLPGILST RWLVRIIST+L+CLIHSRF+L DHELHQELI +G+ERNSM  
Sbjct: 241 AFALLLVPLCLPGILSTHRWLVRIISTNLNCLIHSRFSLADHELHQELITIGSERNSMMD 300

Query: 301 TTPFDSKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYVAYFCGGTIGLVYSNN 360
             PF+SKEKESISRKVMEKENLVVLEEEHSA MLMR+LDFWLYY AYFCGGTIGLVYSNN
Sbjct: 301 IIPFESKEKESISRKVMEKENLVVLEEEHSAKMLMRQLDFWLYYAAYFCGGTIGLVYSNN 360

Query: 361 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISATPDFMREKVHFARTGWLAIALVPTPIA 420
           LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISA PDFMREK HFARTGWLAIALVPTPIA
Sbjct: 361 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREKGHFARTGWLAIALVPTPIA 420

Query: 421 FILLAASGSKVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 480
           F+LLAASGSK+ALQAGT LIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF
Sbjct: 421 FVLLAASGSKIALQAGTSLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 480

Query: 481 LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRT 540
           LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYL TFVWWACISIFGLASSFLLFRRT
Sbjct: 481 LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLMTFVWWACISIFGLASSFLLFRRT 540

Query: 541 RPAYDRHYESNLSKMQPF 559
           + AYDRHYESNLSKMQPF
Sbjct: 541 KSAYDRHYESNLSKMQPF 558

BLAST of HG10010826 vs. NCBI nr
Match: XP_004145067.2 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Cucumis sativus])

HSP 1 Score: 1004.6 bits (2596), Expect = 3.4e-289
Identity = 512/558 (91.76%), Postives = 537/558 (96.24%), Query Frame = 0

Query: 1   MLNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60
           M+NQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW
Sbjct: 45  MMNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 104

Query: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNT 120
           CSGVALLYFPLWVVMFMAASMGFLGYG QWLLL RI+SLPYSMVY+LCLMAGCSICWFNT
Sbjct: 105 CSGVALLYFPLWVVMFMAASMGFLGYGFQWLLLQRIISLPYSMVYLLCLMAGCSICWFNT 164

Query: 121 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDASLYLFLNALVPLIISV 180
           VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLI+NAIDP+DASLYLFLNALVPLIISV
Sbjct: 165 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAIDPSDASLYLFLNALVPLIISV 224

Query: 181 VALLPILHQPPVQPSSADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQILLAG 240
           VALLP+LH+PPVQPSS DAIRHDSLIFICLY+ A+ITGLYLITFNSMPSNKYGSQILLAG
Sbjct: 225 VALLPMLHKPPVQPSSDDAIRHDSLIFICLYMTAIITGLYLITFNSMPSNKYGSQILLAG 284

Query: 241 AFALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNSMKV 300
           AFALL+VPLCLPG+LST RWLVRIIST+L+CLIHSRF+LVDHELHQELI + +ERNSMK 
Sbjct: 285 AFALLIVPLCLPGVLSTHRWLVRIISTTLNCLIHSRFSLVDHELHQELITIESERNSMKG 344

Query: 301 TTPFDSKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYVAYFCGGTIGLVYSNN 360
             PF+SKEKESISRKVMEKENLVVLEEEHSA MLMR+LDFWLYY AYFCGGTIGLVYSN+
Sbjct: 345 IVPFESKEKESISRKVMEKENLVVLEEEHSAKMLMRQLDFWLYYAAYFCGGTIGLVYSNS 404

Query: 361 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISATPDFMREKVHFARTGWLAIALVPTPIA 420
           LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISA PDFMRE+ HFARTGWLAIALVPTPIA
Sbjct: 405 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMRERGHFARTGWLAIALVPTPIA 464

Query: 421 FILLAASGSKVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 480
           FILLAASGSK+ALQ GT LIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF
Sbjct: 465 FILLAASGSKIALQVGTSLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 524

Query: 481 LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRT 540
           LYGVLAA+AYDSNAGSSHQT+ALGDAVVCIGQNCYL TFVWWACISIFGLA SFLLFRRT
Sbjct: 525 LYGVLAAMAYDSNAGSSHQTSALGDAVVCIGQNCYLMTFVWWACISIFGLACSFLLFRRT 584

Query: 541 RPAYDRHYESNLSKMQPF 559
           + AYDRHY+SN S MQPF
Sbjct: 585 KSAYDRHYDSNPSNMQPF 602

BLAST of HG10010826 vs. NCBI nr
Match: XP_023515123.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 935.6 bits (2417), Expect = 1.9e-268
Identity = 481/559 (86.05%), Postives = 514/559 (91.95%), Query Frame = 0

Query: 1   MLNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60
           M+NQPGKWV LVAAIWIQAF GTNFDFPSYSS+LKAALGMSQVELNYLAVASD+GKAFGW
Sbjct: 22  MMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALGMSQVELNYLAVASDVGKAFGW 81

Query: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNT 120
           CSG+ALLYFPLWVVM MAASMGFLGYGLQWLLL  IVSLPYSMVY+LCL+AGCSICWFNT
Sbjct: 82  CSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSMVYLLCLLAGCSICWFNT 141

Query: 121 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDASLYLFLNALVPLIISV 180
           +CYVSCIQ FPANRALALSLIVSFNGVSAALYTL++NA+DPNDASLYL LNALVPLIISV
Sbjct: 142 ICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMANAVDPNDASLYLLLNALVPLIISV 201

Query: 181 VALLPILHQPPVQPSSADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQILLAG 240
           V  LPILHQPP QPSS+DA R+DS  FICLYI AVITGLYLITFNS PS+KYG+Q+LLAG
Sbjct: 202 VTFLPILHQPPAQPSSSDATRNDSHTFICLYITAVITGLYLITFNSTPSSKYGAQLLLAG 261

Query: 241 AFALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNSMKV 300
           AFALLLVPLCLPGI ST +WL RI ST LS ++H RFNLV+HEL QELI + TER+   V
Sbjct: 262 AFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNLVNHELQQELINLETERSGTNV 321

Query: 301 TTPFDSKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYVAYFCGGTIGLVYSNN 360
            TPFD KEKESISRK+M KENL VLEEEHSA ML+RRLDFWLY+VAYFCGGTIGLVYSNN
Sbjct: 322 ITPFDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLDFWLYFVAYFCGGTIGLVYSNN 381

Query: 361 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISATPDFMREKVHFARTGWLAIALVPTPIA 420
           LGQI+QSLGYSS TSSLVTLYSSCSFFGRLISA PDFMREKVHFARTGWLA+ALVPTPIA
Sbjct: 382 LGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMREKVHFARTGWLALALVPTPIA 441

Query: 421 FILLAASGSKVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 480
           FILLAASGS++ALQ GTGLIGLSSGF+FSASVSITSELFGPNSSGVNHNILITNIPLGSF
Sbjct: 442 FILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELFGPNSSGVNHNILITNIPLGSF 501

Query: 481 LYGVLAAVAYDSNAGSSHQT-AALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRR 540
           LYGVLAAV+YDS AGSS QT AALGD VVCIG+ CYLQTF+WWACISI GLASSFLLFRR
Sbjct: 502 LYGVLAAVSYDSTAGSSDQTAAALGDVVVCIGRKCYLQTFIWWACISIVGLASSFLLFRR 561

Query: 541 TRPAYDRHYESNLSKMQPF 559
           T+PAYDR YESN SK QPF
Sbjct: 562 TKPAYDRQYESNPSKTQPF 580

BLAST of HG10010826 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 1.3e-158
Identity = 307/549 (55.92%), Postives = 398/549 (72.50%), Query Frame = 0

Query: 7   KWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGWCSGVAL 66
           KW VLVAAIWIQA  GTNFDF +YSS LK+ LG+SQV LNYLAVASDLGKAFGW SG+AL
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 67  LYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNTVCYVSC 126
            YFPL VV+F AA+MGF+GYG+QWL++  I++LPYS+V++ CL+AG SICWFNT C++ C
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCLLAGLSICWFNTACFILC 162

Query: 127 IQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDASLYLFLNALVPLIISVVALLPI 186
           I++FP NRALALSL VSFNG+SAALY+L  NAI+P+ ++LYL LN+LVPL++S  AL P+
Sbjct: 163 IRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALYPV 222

Query: 187 LHQPPVQPS-SADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQILLAGAFALL 246
           L +P +  +   D+ RHDS +F  L + AVIT  +L+  +S  S+   +++   GA  LL
Sbjct: 223 LTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSS---ARLNFIGAVVLL 282

Query: 247 LVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNSMKVTTPFD 306
           + PLC P ++  R + + +I+  L+        L   EL  +  +V ++     + T   
Sbjct: 283 VFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGT--- 342

Query: 307 SKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYVAYFCGGTIGLVYSNNLGQIA 366
                       ++ N V L +EHS  +L+ RL+FWLYY+AYFCGGTIGLVYSNNLGQIA
Sbjct: 343 -----------AKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIA 402

Query: 367 QSLGYSSSTSSLVTLYSSCSFFGRLISATPDFMREKVHFARTGWLAIALVPTPIAFILLA 426
           QSLG +S+T  LVT+YSS SFFGRL+SA PDFM ++    RTGW AIAL+PTPIAF LLA
Sbjct: 403 QSLGQNSTT--LVTIYSSFSFFGRLLSAAPDFMHKRFRLTRTGWFAIALLPTPIAFFLLA 462

Query: 427 ASGS-KVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSFLYGV 486
            S S + ALQ  T LIGLSSGFIF+A+VSITS+LFGPNS GVNHNILITNIP+GS LYG 
Sbjct: 463 VSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY 522

Query: 487 LAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRTRPAY 546
           +AA  Y++NA S   T  + D++VCIG++CY +TFV+W C+SI G+ SS  L+ RT+P Y
Sbjct: 523 IAASIYEANA-SPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 571

Query: 547 DRHYESNLS 554
            R  +  +S
Sbjct: 583 HRLEQDKVS 571

BLAST of HG10010826 vs. ExPASy TrEMBL
Match: A0A1S3CCZ1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103499000 PE=4 SV=1)

HSP 1 Score: 1020.4 bits (2637), Expect = 2.9e-294
Identity = 524/558 (93.91%), Postives = 539/558 (96.59%), Query Frame = 0

Query: 1   MLNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60
           M+NQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW
Sbjct: 22  MMNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 81

Query: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNT 120
           CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLL RI+SLPYS+VY+LCLMAGCSICWFNT
Sbjct: 82  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIISLPYSVVYLLCLMAGCSICWFNT 141

Query: 121 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDASLYLFLNALVPLIISV 180
           VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLI+NAIDP DASLYLFLNALVPLIISV
Sbjct: 142 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAIDPGDASLYLFLNALVPLIISV 201

Query: 181 VALLPILHQPPVQPSSADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQILLAG 240
           VALLP+LHQP VQPSSADAIRHDSLIFICLYI AVITGLYLITFNSMPSNKYGSQILLAG
Sbjct: 202 VALLPMLHQPQVQPSSADAIRHDSLIFICLYITAVITGLYLITFNSMPSNKYGSQILLAG 261

Query: 241 AFALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNSMKV 300
           AFALLLVPLCLPGILST RWLVRIISTSL+CLIHSRF+L DHELHQEL  +G+ERNSM  
Sbjct: 262 AFALLLVPLCLPGILSTHRWLVRIISTSLNCLIHSRFSLADHELHQELFTIGSERNSMMD 321

Query: 301 TTPFDSKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYVAYFCGGTIGLVYSNN 360
             PF+SKEKESISRKVMEKENLVVLEEEHSA MLMR+LDFWLYY AYFCGGTIGLVYSNN
Sbjct: 322 IIPFESKEKESISRKVMEKENLVVLEEEHSAKMLMRQLDFWLYYAAYFCGGTIGLVYSNN 381

Query: 361 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISATPDFMREKVHFARTGWLAIALVPTPIA 420
           LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISA PDFMREK HFARTGWLAIALVPTPIA
Sbjct: 382 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREKGHFARTGWLAIALVPTPIA 441

Query: 421 FILLAASGSKVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 480
           F+LLAASGSK+ALQAGT LIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF
Sbjct: 442 FVLLAASGSKIALQAGTSLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 501

Query: 481 LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRT 540
           LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYL TFVWWACISIFGLASSFLLFRRT
Sbjct: 502 LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLMTFVWWACISIFGLASSFLLFRRT 561

Query: 541 RPAYDRHYESNLSKMQPF 559
           + AYDRHYESNLSKMQPF
Sbjct: 562 KSAYDRHYESNLSKMQPF 579

BLAST of HG10010826 vs. ExPASy TrEMBL
Match: A0A5A7TFE8 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00650 PE=4 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 8.4e-294
Identity = 523/558 (93.73%), Postives = 540/558 (96.77%), Query Frame = 0

Query: 1   MLNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60
           M+NQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW
Sbjct: 1   MMNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60

Query: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNT 120
           CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLL RI+SLPYS+VY+LCLMAGCSICWFNT
Sbjct: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLQRIISLPYSVVYLLCLMAGCSICWFNT 120

Query: 121 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDASLYLFLNALVPLIISV 180
           VCYVSCIQNFPA+RALALSLIVSFNGVSAALYTLI+NAIDP DASLYLFLNALVPLIISV
Sbjct: 121 VCYVSCIQNFPAHRALALSLIVSFNGVSAALYTLIANAIDPGDASLYLFLNALVPLIISV 180

Query: 181 VALLPILHQPPVQPSSADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQILLAG 240
           VALLP+LHQP VQPSSADAIRHDSLIFICLYI AVITGLYLITFNSMPSNKYGSQILLAG
Sbjct: 181 VALLPMLHQPQVQPSSADAIRHDSLIFICLYITAVITGLYLITFNSMPSNKYGSQILLAG 240

Query: 241 AFALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNSMKV 300
           AFALLLVPLCLPGILST RWLVRIIST+L+CLIHSRF+L DHELHQELI +G+ERNSM  
Sbjct: 241 AFALLLVPLCLPGILSTHRWLVRIISTNLNCLIHSRFSLADHELHQELITIGSERNSMMD 300

Query: 301 TTPFDSKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYVAYFCGGTIGLVYSNN 360
             PF+SKEKESISRKVMEKENLVVLEEEHSA MLMR+LDFWLYY AYFCGGTIGLVYSNN
Sbjct: 301 IIPFESKEKESISRKVMEKENLVVLEEEHSAKMLMRQLDFWLYYAAYFCGGTIGLVYSNN 360

Query: 361 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISATPDFMREKVHFARTGWLAIALVPTPIA 420
           LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISA PDFMREK HFARTGWLAIALVPTPIA
Sbjct: 361 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMREKGHFARTGWLAIALVPTPIA 420

Query: 421 FILLAASGSKVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 480
           F+LLAASGSK+ALQAGT LIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF
Sbjct: 421 FVLLAASGSKIALQAGTSLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 480

Query: 481 LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRT 540
           LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYL TFVWWACISIFGLASSFLLFRRT
Sbjct: 481 LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLMTFVWWACISIFGLASSFLLFRRT 540

Query: 541 RPAYDRHYESNLSKMQPF 559
           + AYDRHYESNLSKMQPF
Sbjct: 541 KSAYDRHYESNLSKMQPF 558

BLAST of HG10010826 vs. ExPASy TrEMBL
Match: A0A0A0KCZ5 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G078620 PE=4 SV=1)

HSP 1 Score: 1004.6 bits (2596), Expect = 1.6e-289
Identity = 512/558 (91.76%), Postives = 537/558 (96.24%), Query Frame = 0

Query: 1   MLNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60
           M+NQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW
Sbjct: 45  MMNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 104

Query: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNT 120
           CSGVALLYFPLWVVMFMAASMGFLGYG QWLLL RI+SLPYSMVY+LCLMAGCSICWFNT
Sbjct: 105 CSGVALLYFPLWVVMFMAASMGFLGYGFQWLLLQRIISLPYSMVYLLCLMAGCSICWFNT 164

Query: 121 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDASLYLFLNALVPLIISV 180
           VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLI+NAIDP+DASLYLFLNALVPLIISV
Sbjct: 165 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLIANAIDPSDASLYLFLNALVPLIISV 224

Query: 181 VALLPILHQPPVQPSSADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQILLAG 240
           VALLP+LH+PPVQPSS DAIRHDSLIFICLY+ A+ITGLYLITFNSMPSNKYGSQILLAG
Sbjct: 225 VALLPMLHKPPVQPSSDDAIRHDSLIFICLYMTAIITGLYLITFNSMPSNKYGSQILLAG 284

Query: 241 AFALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNSMKV 300
           AFALL+VPLCLPG+LST RWLVRIIST+L+CLIHSRF+LVDHELHQELI + +ERNSMK 
Sbjct: 285 AFALLIVPLCLPGVLSTHRWLVRIISTTLNCLIHSRFSLVDHELHQELITIESERNSMKG 344

Query: 301 TTPFDSKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYVAYFCGGTIGLVYSNN 360
             PF+SKEKESISRKVMEKENLVVLEEEHSA MLMR+LDFWLYY AYFCGGTIGLVYSN+
Sbjct: 345 IVPFESKEKESISRKVMEKENLVVLEEEHSAKMLMRQLDFWLYYAAYFCGGTIGLVYSNS 404

Query: 361 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISATPDFMREKVHFARTGWLAIALVPTPIA 420
           LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISA PDFMRE+ HFARTGWLAIALVPTPIA
Sbjct: 405 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISAAPDFMRERGHFARTGWLAIALVPTPIA 464

Query: 421 FILLAASGSKVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 480
           FILLAASGSK+ALQ GT LIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF
Sbjct: 465 FILLAASGSKIALQVGTSLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 524

Query: 481 LYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRT 540
           LYGVLAA+AYDSNAGSSHQT+ALGDAVVCIGQNCYL TFVWWACISIFGLA SFLLFRRT
Sbjct: 525 LYGVLAAMAYDSNAGSSHQTSALGDAVVCIGQNCYLMTFVWWACISIFGLACSFLLFRRT 584

Query: 541 RPAYDRHYESNLSKMQPF 559
           + AYDRHY+SN S MQPF
Sbjct: 585 KSAYDRHYDSNPSNMQPF 602

BLAST of HG10010826 vs. ExPASy TrEMBL
Match: A0A6J1KEQ9 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111494334 PE=4 SV=1)

HSP 1 Score: 935.3 bits (2416), Expect = 1.2e-268
Identity = 481/559 (86.05%), Postives = 514/559 (91.95%), Query Frame = 0

Query: 1   MLNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60
           M+NQPGKWV L+AAIWIQAF GTNFDFPSYSS+LKAALGMSQVELNYLAVASD+GKAFGW
Sbjct: 22  MMNQPGKWVALMAAIWIQAFTGTNFDFPSYSSNLKAALGMSQVELNYLAVASDVGKAFGW 81

Query: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNT 120
           CSG+ALLYFP WVVM MAASMGFLGYGLQWLLL  IVSLPYSMVY+LCL+AGCSICWFNT
Sbjct: 82  CSGIALLYFPSWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSMVYLLCLLAGCSICWFNT 141

Query: 121 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDASLYLFLNALVPLIISV 180
           +CYVSCIQ FPANRALALSLIVSFNGVSAALYTL++NA+DPNDASLYL LNALVPLIISV
Sbjct: 142 ICYVSCIQKFPANRALALSLIVSFNGVSAALYTLMTNAVDPNDASLYLLLNALVPLIISV 201

Query: 181 VALLPILHQPPVQPSSADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQILLAG 240
           VA  PILHQPP QPSSADA R+DSL FICLYI AVITGLYLITFNS PS+KYG+QILLAG
Sbjct: 202 VAFFPILHQPPAQPSSADATRNDSLTFICLYITAVITGLYLITFNSTPSSKYGAQILLAG 261

Query: 241 AFALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNSMKV 300
           AFALLLVPLCLPGI ST +WL RI ST LS ++H RFNLV+HEL QELI V +ER+  KV
Sbjct: 262 AFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNLVNHELQQELINVESERSGTKV 321

Query: 301 TTPFDSKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYVAYFCGGTIGLVYSNN 360
            TP D KEKESISRK+M KENL VLEEEHSA ML+RRLDFWLY+VAYFCGGTIGLVYSNN
Sbjct: 322 ITPCDYKEKESISRKMMGKENLTVLEEEHSAKMLVRRLDFWLYFVAYFCGGTIGLVYSNN 381

Query: 361 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISATPDFMREKVHFARTGWLAIALVPTPIA 420
           LGQI+QSLGYSS TSSLVTLYSSCSFFGRLISA PDFMREKVHFARTGWLA+ALVP PIA
Sbjct: 382 LGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMREKVHFARTGWLALALVPNPIA 441

Query: 421 FILLAASGSKVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 480
           FILLAASGS++ALQ GTGLIGLSSGF+FSASVSITSELFGPNSSGVNHNILITNIPLGSF
Sbjct: 442 FILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELFGPNSSGVNHNILITNIPLGSF 501

Query: 481 LYGVLAAVAYDSNAGSSHQTAA-LGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRR 540
           LYGVLAAV+YDS AGSS+QTAA LGD VVCIG+ CYLQTF+WWACISI GLAS FLLFRR
Sbjct: 502 LYGVLAAVSYDSTAGSSNQTAATLGDVVVCIGRKCYLQTFIWWACISIVGLASCFLLFRR 561

Query: 541 TRPAYDRHYESNLSKMQPF 559
           T+PAYDRHYESN SK QPF
Sbjct: 562 TKPAYDRHYESNPSKTQPF 580

BLAST of HG10010826 vs. ExPASy TrEMBL
Match: A0A6J1HJV4 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464237 PE=4 SV=1)

HSP 1 Score: 935.3 bits (2416), Expect = 1.2e-268
Identity = 479/555 (86.31%), Postives = 513/555 (92.43%), Query Frame = 0

Query: 1   MLNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60
           M+NQPGKWV LVAAIWIQAF GTNFDFPSYSS+LKAALGMSQVELNYLAVASD+GKAFGW
Sbjct: 22  MMNQPGKWVALVAAIWIQAFTGTNFDFPSYSSNLKAALGMSQVELNYLAVASDVGKAFGW 81

Query: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNT 120
           CSG+ALLYFPLWVVM MAASMGFLGYGLQWLLL  IVSLPYSMVY+LCL+AGCSICWFNT
Sbjct: 82  CSGIALLYFPLWVVMLMAASMGFLGYGLQWLLLQGIVSLPYSMVYLLCLLAGCSICWFNT 141

Query: 121 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDASLYLFLNALVPLIISV 180
           +CYVSCIQ FP NRALALSLIVSFNGVSAALYTL++NA+DPNDASLYL LNALVPLIISV
Sbjct: 142 ICYVSCIQKFPTNRALALSLIVSFNGVSAALYTLMANALDPNDASLYLLLNALVPLIISV 201

Query: 181 VALLPILHQPPVQPSSADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQILLAG 240
           V  LPILHQPP QPSSADA R+DSL FICLYI AVITGLYLITFNS PS+KYG+Q+LLAG
Sbjct: 202 VTFLPILHQPPAQPSSADATRNDSLTFICLYITAVITGLYLITFNSTPSSKYGAQLLLAG 261

Query: 241 AFALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNSMKV 300
           AFALLLVPLCLPGI ST +WL RI ST LS ++H RFNLV+HEL QELI + TER+  KV
Sbjct: 262 AFALLLVPLCLPGIWSTHKWLFRIASTGLSSVLHFRFNLVNHELQQELIPIETERSGTKV 321

Query: 301 TTPFDSKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYVAYFCGGTIGLVYSNN 360
            TPFD KEKESISRK+M KENL VLEEEHSA ML+RRLDFWLY+VAYFCGGTIGLVYSNN
Sbjct: 322 ITPFDYKEKESISRKMMGKENLTVLEEEHSAKMLIRRLDFWLYFVAYFCGGTIGLVYSNN 381

Query: 361 LGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISATPDFMREKVHFARTGWLAIALVPTPIA 420
           LGQI+QSLGYSS TSSLVTLYSSCSFFGRLISA PDFMREKVHFARTGWLA+ALVPTPIA
Sbjct: 382 LGQISQSLGYSSLTSSLVTLYSSCSFFGRLISAGPDFMREKVHFARTGWLALALVPTPIA 441

Query: 421 FILLAASGSKVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSF 480
           FILLAASGS++ALQ GTGLIGLSSGF+FSASVSITSELFGPNSSGVNHNILITNIPLGSF
Sbjct: 442 FILLAASGSEIALQIGTGLIGLSSGFMFSASVSITSELFGPNSSGVNHNILITNIPLGSF 501

Query: 481 LYGVLAAVAYDSNAGSSHQT-AALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRR 540
           LYGVLAAV+YDS AGSS+QT AALGD VVCIG+ CYLQTF+WWACISI GLAS FLLFRR
Sbjct: 502 LYGVLAAVSYDSTAGSSNQTAAALGDVVVCIGRKCYLQTFIWWACISIIGLASCFLLFRR 561

Query: 541 TRPAYDRHYESNLSK 555
           T+PAYDRHYESN +K
Sbjct: 562 TKPAYDRHYESNPNK 576

BLAST of HG10010826 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 645.2 bits (1663), Expect = 4.8e-185
Identity = 339/568 (59.68%), Postives = 423/568 (74.47%), Query Frame = 0

Query: 1   MLNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60
           M  Q  KW++LVA IWIQAF GTNFDF +YSSDLK+ LG+SQV+LNYLAVASDLGK FGW
Sbjct: 1   MAGQSRKWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGW 60

Query: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNT 120
            SG+AL+YFPLW V+F AA MGF+GYG+QWL++   +SLPY MV++ CL+AG SICWFNT
Sbjct: 61  SSGLALMYFPLWTVLFAAAFMGFVGYGVQWLVITHFISLPYIMVFLCCLLAGLSICWFNT 120

Query: 121 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDASLYLFLNALVPLIISV 180
           VC+V CI NFPANR+LALSL VSFNGVSAALYTL  NAI+P    LYL LNAL+PLI+S 
Sbjct: 121 VCFVLCISNFPANRSLALSLTVSFNGVSAALYTLAYNAINPTSPELYLLLNALIPLIVSF 180

Query: 181 VALLPILHQPPVQPSSADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQILLAG 240
            A++PIL QPP +P   D +R DSL+F+ L I A + G+YL+ F S  S+   +++L  G
Sbjct: 181 TAIIPILRQPPFEPLPPDGVRRDSLMFLLLNILAALNGVYLLLFGSNSSDLTSARLLFGG 240

Query: 241 AFALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDH---ELHQELIAVGTER-- 300
           A  LL+ PLC+PG++  R W  R I TS   L  S F LVD    ELH+ ++A    R  
Sbjct: 241 AILLLVFPLCIPGLVIARNWYNRTIHTSFR-LEGSGFILVDPDELELHKGMLAHEANREG 300

Query: 301 ----------NSMKVTTPFDSKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYV 360
                     N +K     +    ES  +K++ ++ L  L  EHS ++L+ R DFWLYY+
Sbjct: 301 YQLLSDDVVQNPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRSDFWLYYI 360

Query: 361 AYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISATPDFMREKVHFA 420
            YFCGGTIGLVYSNNLGQIAQSLG SS+T++LVTLYS+ SFFGRL+SATPD++R KV+FA
Sbjct: 361 TYFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPDYIRAKVYFA 420

Query: 421 RTGWLAIALVPTPIAFILLAASGSKVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSG 480
           RTGWLAIAL+PTP A  LLA+SG+  ALQAGT L+GLSSGFIF+A+VSITSELFGPNS G
Sbjct: 421 RTGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSITSELFGPNSVG 480

Query: 481 VNHNILITNIPLGSFLYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWACI 540
           VNHNILITNIP+GS +YG LAA+ YDS+ G +   +   ++VVC+G++CY  TFVWW C+
Sbjct: 481 VNHNILITNIPIGSLIYGFLAALVYDSH-GFTGTKSMTSESVVCMGRDCYYLTFVWWGCL 540

Query: 541 SIFGLASSFLLFRRTRPAYDRHYESNLS 554
           S+ GL SS +LF RTR AY R  ++ +S
Sbjct: 541 SLLGLGSSLVLFIRTRRAYQRFEQARIS 566

BLAST of HG10010826 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 641.0 bits (1652), Expect = 9.1e-184
Identity = 338/569 (59.40%), Postives = 426/569 (74.87%), Query Frame = 0

Query: 1   MLNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60
           M  Q  KW++LVA IWIQAF GTNFDF +YSS+LK+ LG+SQV+LNYLAVASDLGK FGW
Sbjct: 1   MAGQSRKWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGW 60

Query: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNT 120
            SG+ALLYFPLW V+F AA MGF+GYG+QWL++  ++SLPY +V++ CL+AG SICWFNT
Sbjct: 61  SSGLALLYFPLWTVLFAAAIMGFVGYGVQWLVITNVISLPYILVFLCCLLAGLSICWFNT 120

Query: 121 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDASLYLFLNALVPLIISV 180
           VC+V CI+NFPANR+LALSL VSFNGVSAALYTL  NAI+P    LYL LNALVPL +S 
Sbjct: 121 VCFVLCIRNFPANRSLALSLTVSFNGVSAALYTLAYNAINPVSTELYLLLNALVPLFVSF 180

Query: 181 VALLPILHQPPVQPSSADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQILLAG 240
            AL+PIL QPP++P   D +R DSL+F+ L I AV+ G+YL+ F S  S+   +++L  G
Sbjct: 181 AALIPILRQPPLEPLPPDGVRRDSLMFLLLNILAVLNGVYLLLFRSKTSDVTSARLLFGG 240

Query: 241 AFALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVD---HELHQELIAVGTERNS 300
           +  LL++PLCLPG++  R W +  I +S   L  S F LVD    E+H+ ++        
Sbjct: 241 SILLLILPLCLPGLVYARNWYLHNIHSSFR-LEGSGFILVDVDELEMHKGMVTREASLEG 300

Query: 301 MK---------VTTP----FDSKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYY 360
            +         V TP    F   +      KV+ +  L +L EEH  + L+ R DFWLYY
Sbjct: 301 YQLLNDDVVRAVNTPDQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRSDFWLYY 360

Query: 361 VAYFCGGTIGLVYSNNLGQIAQSLGYSSSTSSLVTLYSSCSFFGRLISATPDFMREKVHF 420
           +AYFCGGTIGLVYSNNLGQIAQSLG SS T++LVTLYSS SFFGRL+SATPD++R KV+F
Sbjct: 361 IAYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPDYIRAKVYF 420

Query: 421 ARTGWLAIALVPTPIAFILLAASGSKVALQAGTGLIGLSSGFIFSASVSITSELFGPNSS 480
           ARTGWLA+AL+PT IA  LLA+SGS  ALQAGT LIGLSSGFIF+A+VSITSELFGPNS 
Sbjct: 421 ARTGWLAVALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSELFGPNSV 480

Query: 481 GVNHNILITNIPLGSFLYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWAC 540
           GVNHNILITNIP+GS +YG LAA+ Y+S++ +  +T    ++V+C+G++CYLQTF+WW C
Sbjct: 481 GVNHNILITNIPIGSLVYGFLAALVYESHSVAGSKT----ESVICMGRDCYLQTFMWWGC 540

Query: 541 ISIFGLASSFLLFRRTRPAYDRHYESNLS 554
           +S+ GLASS +LF RTR AY R  +  ++
Sbjct: 541 LSVIGLASSVVLFLRTRRAYQRFEQDRIT 564

BLAST of HG10010826 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 561.2 bits (1445), Expect = 9.2e-160
Identity = 307/549 (55.92%), Postives = 398/549 (72.50%), Query Frame = 0

Query: 7   KWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGWCSGVAL 66
           KW VLVAAIWIQA  GTNFDF +YSS LK+ LG+SQV LNYLAVASDLGKAFGW SG+AL
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 67  LYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNTVCYVSC 126
            YFPL VV+F AA+MGF+GYG+QWL++  I++LPYS+V++ CL+AG SICWFNT C++ C
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCLLAGLSICWFNTACFILC 162

Query: 127 IQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDASLYLFLNALVPLIISVVALLPI 186
           I++FP NRALALSL VSFNG+SAALY+L  NAI+P+ ++LYL LN+LVPL++S  AL P+
Sbjct: 163 IRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALYPV 222

Query: 187 LHQPPVQPS-SADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQILLAGAFALL 246
           L +P +  +   D+ RHDS +F  L + AVIT  +L+  +S  S+   +++   GA  LL
Sbjct: 223 LTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSS---ARLNFIGAVVLL 282

Query: 247 LVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNSMKVTTPFD 306
           + PLC P ++  R + + +I+  L+        L   EL  +  +V ++     + T   
Sbjct: 283 VFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGT--- 342

Query: 307 SKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYVAYFCGGTIGLVYSNNLGQIA 366
                       ++ N V L +EHS  +L+ RL+FWLYY+AYFCGGTIGLVYSNNLGQIA
Sbjct: 343 -----------AKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIA 402

Query: 367 QSLGYSSSTSSLVTLYSSCSFFGRLISATPDFMREKVHFARTGWLAIALVPTPIAFILLA 426
           QSLG +S+T  LVT+YSS SFFGRL+SA PDFM ++    RTGW AIAL+PTPIAF LLA
Sbjct: 403 QSLGQNSTT--LVTIYSSFSFFGRLLSAAPDFMHKRFRLTRTGWFAIALLPTPIAFFLLA 462

Query: 427 ASGS-KVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGSFLYGV 486
            S S + ALQ  T LIGLSSGFIF+A+VSITS+LFGPNS GVNHNILITNIP+GS LYG 
Sbjct: 463 VSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY 522

Query: 487 LAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRRTRPAY 546
           +AA  Y++NA S   T  + D++VCIG++CY +TFV+W C+SI G+ SS  L+ RT+P Y
Sbjct: 523 IAASIYEANA-SPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 571

Query: 547 DRHYESNLS 554
            R  +  +S
Sbjct: 583 HRLEQDKVS 571

BLAST of HG10010826 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 492.7 bits (1267), Expect = 4.0e-139
Identity = 283/556 (50.90%), Postives = 371/556 (66.73%), Query Frame = 0

Query: 1   MLNQPGKWVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGW 60
           ML    +W VLVAAIWIQAF GTNFDF +YSSD+K+++G+SQ  LNY+AVASDLGKA GW
Sbjct: 17  MLQWWRRWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSRLNYMAVASDLGKALGW 76

Query: 61  CSGVALLYFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNT 120
            SG A+ YFP+  V+F AA+MG +GYG+QWL +  ++ LPYS+V + C +AG SICWFNT
Sbjct: 77  SSGFAIAYFPVSGVLFAAAAMGLVGYGVQWLSIADVIDLPYSLVLVCCSLAGLSICWFNT 136

Query: 121 VCYVSCIQNFPANRALALSLIVSFNGVSAALYTLISNAIDPNDAS---LYLFLNALVPLI 180
           V ++ CI++F AN +LALSL+VSFNG+SAALYTL   AI    ++   +YL LN+L+PLI
Sbjct: 137 VSFILCIRHFKANHSLALSLVVSFNGISAALYTLGHEAISGKSSASSDIYLLLNSLIPLI 196

Query: 181 ISVVALLPILHQPPVQPSSADAIRHDSLIFICLYIAAVITGLYLITFNSMPSNKYGSQIL 240
           +SV+AL P+L  P    +       ++ IF+   + A++T  YL+  +S        +  
Sbjct: 197 VSVLALWPVLTNPNSSETDTTRTHDETRIFVVFNVLALVTCFYLLLPSSGTYLASSPRWH 256

Query: 241 LAGAFALLLVPLCLPGILSTRRWLVRIISTSLSCLIH--SRFNLVDHELHQELIAVGTER 300
             GA  LLL PLC+P +    R L        SC  H  S +  V+ E          E 
Sbjct: 257 FLGAIFLLLFPLCVPFLDYIHRALE-------SCFHHHSSGYAAVNIE----------EP 316

Query: 301 NSMKVTTPFDSKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYVAYFCGGTIGL 360
             +K+ +   + E+ES         + V L +EHS  ML+R+L+FWLYYVAYFCGGTIGL
Sbjct: 317 KILKIKSQKSNAEEES---------DQVRLGDEHSLGMLVRKLEFWLYYVAYFCGGTIGL 376

Query: 361 VYSNNLGQIAQSLGYSSSTS-SLVTLYSSCSFFGRLISATPDFMREKV-HFARTGWLAIA 420
           VYSNNLGQIAQSLG SSS + SLVTL+S+ SF GRL+S+ PDF R+K+ +  RTGW  I+
Sbjct: 377 VYSNNLGQIAQSLGQSSSNAKSLVTLFSAFSFLGRLLSSAPDFTRKKLKYLTRTGWFTIS 436

Query: 421 LVPTPIAFILLAAS---GSKVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNI 480
           L+PTP+AF +LA S        L+  T LIGLSSGF+F+A+VSITSELFG NS GVN NI
Sbjct: 437 LLPTPLAFFILAYSPKTNQTALLEVATALIGLSSGFVFAAAVSITSELFGRNSVGVNQNI 496

Query: 481 LITNIPLGSFLYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWACISIFGL 540
           LITNIP+GS  YG +A   YD+NA S  + + + D+VVC+G+ CY  TF++W C+S+ G 
Sbjct: 497 LITNIPIGSLFYGYMAGSVYDTNA-SLGRKSVVADSVVCVGRKCYFVTFLFWGCLSVLGF 545

Query: 541 ASSFLLFRRTRPAYDR 547
             S  LF RTRP Y R
Sbjct: 557 VCSLFLFIRTRPVYHR 545

BLAST of HG10010826 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 278.5 bits (711), Expect = 1.2e-74
Identity = 195/547 (35.65%), Postives = 295/547 (53.93%), Query Frame = 0

Query: 8   WVVLVAAIWIQAFAGTNFDFPSYSSDLKAALGMSQVELNYLAVASDLGKAFGWCSGVALL 67
           W+ LVA +W+Q+F GTN  FP+YSS LK  L +SQ +LNYL+ ASD GK  G+ SG+A +
Sbjct: 8   WLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGFISGIAAV 67

Query: 68  YFPLWVVMFMAASMGFLGYGLQWLLLLRIVSLPYSMVYILCLMAGCSICWFNTVCYVSCI 127
           Y PL +V+    S+GF GYGLQ+L +++ +                 ICW NT CY+  I
Sbjct: 68  YLPLPLVLLAGGSLGFAGYGLQYLSIIKKI-----------------ICWINTACYIVAI 127

Query: 128 QNFPANRALALSLIVSFNGVSAALYTLISNAI----DPNDASLYLFLNALVPLIISVVAL 187
            +FP NR +A+ +  S+ G+S  +YT + ++        +AS YL LN+LVPL+  +V  
Sbjct: 128 NSFPVNRQVAVGITASYQGLSGKIYTDMVHSFFHTSQREEASGYLLLNSLVPLVACLVTA 187

Query: 188 LPILHQPPVQPSSADAIRHDSLIFICLYIAAVITGLYLITFN--SMPSNKYGSQILLAGA 247
             ++     +  S    +   + FI L++  + TG+Y +  +  S+P     + ++L G 
Sbjct: 188 PMLMRHGGDKTMSYS--KDVKVGFIVLFVLTIATGIYAVATSLVSVP-----AVLVLVGI 247

Query: 248 FALLLVPLCLPGILSTRRWLVRIISTSLSCLIHSRFNLVDHELHQELIAVGTERNSMKVT 307
              LL PL +P            I      L+ SR      +  Q++  +    +     
Sbjct: 248 ALFLLAPLAIP------------IGVGFKELMSSR------KTQQKVHDLEAPVDKFYFV 307

Query: 308 TPFDSKEKESISRKVMEKENLVVLEEEHSATMLMRRLDFWLYYVAYFCGGTIGLVYSNNL 367
               +KE+E   + +      + ++EE   T L ++LDFW+Y+  Y  G T+GLV++NNL
Sbjct: 308 EEDHTKEEEEFEKAI------IGVKEEVEWTQLWKKLDFWIYFGLYLFGPTVGLVFTNNL 367

Query: 368 GQIAQSLGYSSSTSSLVTLYSSCSFFGRLISATPD--FMREKVHFARTGWLAIALVPTPI 427
           GQIA+S G S++TSSLV L SS  FFGRL+ +  D  F R K   +    +A +LV    
Sbjct: 368 GQIAESRG-STATSSLVALSSSFGFFGRLLPSLLDYFFSRNKYMPSSPVSMAGSLVAMVA 427

Query: 428 AFILLAASGSKVALQAGTGLIGLSSGFIFSASVSITSELFGPNSSGVNHNILITNIPLGS 487
           +F+LL    S +AL  GT +IG+ SG + S SV++T+ELFG    GVNHNI++ +IPLGS
Sbjct: 428 SFLLLLID-SDIALYIGTAMIGIFSGALTSLSVTMTAELFGTKHFGVNHNIVVGSIPLGS 487

Query: 488 FLYGVLAAVAYDSNAGSSHQTAALGDAVVCIGQNCYLQTFVWWACISIFGLASSFLLFRR 547
           F +G+LAA  Y   A      A  GD   C G +C+  T V+W  +       + +L+ R
Sbjct: 488 FSFGLLAAKIYRDGA------ALYGDDGKCFGMHCFQTTLVFWGMLCSIAALLAAVLYIR 498