HG10010036 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10010036
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	RNase H domain-containing protein
Location	Chr06: 17361786 .. 17363332 (+)
RNA-Seq Expression	HG10010036
Synteny	HG10010036

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGGGTAATCTTCAAGCCAAATTGATGTTAGTTGATGTTTGCAGGTATGTGTAGCAAGCTCGTATACAACATAATTGTAGTTGCGATTAACAAAGGAAAAACCTTGGATTGTCCTGCTACGATATAGATCACTCAAAGAATCTACACAATCCTGAAATCTCAATAGTTAAAGTTAAAAGGTTGATAACTCGCATTAAAAGAGGATTAAAAAAAAAAAATACATCATAGTTAAAACCGCTTTTTCAAGAAACGACTCGCATGAGGAAACCAAGTTTTGAACAAATACAGTTTCATAGACTGCCGAAAGAAAAACCATATTACTACAAATAGTTTCAAGTATAATATTTTCTTTATTAATAAAATGTATGGAAGGATATAATCTGAAATTTCAAAATTTTTGTTAAAGAATATGACAGACTTTAAGGAAAGTTAGTTTCAAAAGCAACATCTAGCTATGTGATATATAAAGTTAATTTGTTAGAATTAATTTTTTTTTAAAAACTTTATACAAATTCATTTTAGTGTTTAGAAGATAATATTGATAATCTATATTAGTCATTTATCAATCAATTAATAATGGGGGTAGGAACTGAAAAAGGTATATACCAACCAAGAAGATAAAACTAATTAATAAAAAATATTATTTGTCTCTCAAATCATACCAACCAACCACACAAAACTAATCAAAGGTACGTTCTGTCTCACAAAATAAAAGTTTTCAACGACTCAAATTAGATATAGGGTCGAGTGAATAAATTAAAAAAAGTATACTAATTATCAACCTAACATGTTATAGAGCAACCCTAGGGTTCATTATGAACTGTGCACTCATCTTTATACAATCTATTCCGAAGTCCAATCACACATAAAAAAAAATTATTTTTCTTTAAAAAAAAAAAAAAATTTGCCACATGACAAACTATAGTTAGATCATAAGATGAGCTGCATAAATATAAATGCAGTCAAGATACATAAGTTTTTTTTTATATAATAATTTTCCTATATCTTTGCCATTTGAGTACAACTTCACCTAGAATTAGTTATGATCATTTATTAAATGTCTAGAAATTACTCTACCATATATATCAATATATTGACATCATCATGTAGTTGACTTTGGTGCAATTATTGGTACCCTCTTGGGGTCCATTGTGGCTTCTATTCACGGGTTCAGAGTCTCCTCTTTAAGCTCTTTGTGCGCTGAAGCTTATGCCATTTATGAGAACCTTCATTTAGTTGAAAGGCTGGAGCTTTCCCGTTTGTTGGTGTTATCTGATTCATTATCTGCGGTTCACATGATCAATGGTCTTGAGGAAGCTGAGGTGGACGGTGCAAACACTGTGATGGATATCCGGAAGATCTATGGAGCTTTTGCTTCAATTACCTTTCAGCATGTGTCCCGGACTCATAATTTTGCGGCTCACTCGCTTGTCAAGTTAGGTTCTTCTGGCTCTAGTGCCTTGTGGTTCTGCCAGGCTCCGCCTGGGATATCATCCTTTTATTTGAATGATTTAAACTTTATTTCGGGCTATTTGACTTTGTAA

mRNA sequence

ATGGTGGGTAATCTTCAAGCCAAATTGATGTTAGTTGATGTTTGCAGAGTCTCCTCTTTAAGCTCTTTGTGCGCTGAAGCTTATGCCATTTATGAGAACCTTCATTTAGTTGAAAGGCTGGAGCTTTCCCGTTTGTTGGTGTTATCTGATTCATTATCTGCGGTTCACATGATCAATGGTCTTGAGGAAGCTGAGGTGGACGGTGCAAACACTGTGATGGATATCCGGAAGATCTATGGAGCTTTTGCTTCAATTACCTTTCAGCATGTGTCCCGGACTCATAATTTTGCGGCTCACTCGCTTGTCAAGTTAGGTTCTTCTGGCTCTAGTGCCTTGTGGTTCTGCCAGGCTCCGCCTGGGATATCATCCTTTTATTTGAATGATTTAAACTTTATTTCGGGCTATTTGACTTTGTAA

Coding sequence (CDS)

ATGGTGGGTAATCTTCAAGCCAAATTGATGTTAGTTGATGTTTGCAGAGTCTCCTCTTTAAGCTCTTTGTGCGCTGAAGCTTATGCCATTTATGAGAACCTTCATTTAGTTGAAAGGCTGGAGCTTTCCCGTTTGTTGGTGTTATCTGATTCATTATCTGCGGTTCACATGATCAATGGTCTTGAGGAAGCTGAGGTGGACGGTGCAAACACTGTGATGGATATCCGGAAGATCTATGGAGCTTTTGCTTCAATTACCTTTCAGCATGTGTCCCGGACTCATAATTTTGCGGCTCACTCGCTTGTCAAGTTAGGTTCTTCTGGCTCTAGTGCCTTGTGGTTCTGCCAGGCTCCGCCTGGGATATCATCCTTTTATTTGAATGATTTAAACTTTATTTCGGGCTATTTGACTTTGTAA

Protein sequence

MVGNLQAKLMLVDVCRVSSLSSLCAEAYAIYENLHLVERLELSRLLVLSDSLSAVHMINGLEEAEVDGANTVMDIRKIYGAFASITFQHVSRTHNFAAHSLVKLGSSGSSALWFCQAPPGISSFYLNDLNFISGYLTL

Homology

[Close]

BLAST of HG10010036 vs. NCBI nr
Match: KAF7840958.1 (reverse transcriptase [Senna tora])

HSP 1 Score: 58.9 bits (141), Expect = 3.9e-05
Identity = 41/111 (36.94%), Postives = 62/111 (55.86%), Query Frame = 0


Query: 21  SSLCAEAYAIYENLHLVERLELSRLLVLSDSLSAVHMINGLEEAEVDGANTVMDIRK-IY 80
           +SL AE +AI+E L L +RL    +++ +DSL+AV++I   E    +  +T++ I K   
Sbjct: 746 NSLMAELWAIFEGLKLAKRLFCHNIMIETDSLTAVNLITSCENRNHNQFSTLVSICKATL 805

Query: 81  GAFASITFQHVSRTHNFAAHSLVKLG-SSGSSALWFCQAPPGISSFYLNDL 130
             F+ +   HV R  N  A  L KL  SS S  ++F + PP ISS +  DL
Sbjct: 806 SDFSDVKVVHVHREGNTCADILAKLAVSSRSPLMYFDKLPPCISSAFAADL 856

[Close]

BLAST of HG10010036 vs. NCBI nr
Match: XP_013658645.1 (uncharacterized protein LOC106363457 [Brassica napus])

HSP 1 Score: 57.4 bits (137), Expect = 1.1e-04
Identity = 37/90 (41.11%), Postives = 53/90 (58.89%), Query Frame = 0


Query: 14   VCRVSSLSSLCAEAYAIYENLHLVERLELSRLLVLSDSLSAVHMINGLEEAEVDGANTVM 73
            +C  S  S L AEA A+   + +   LE S L VLSD+L+ +  I+G+ +A+ +    V 
Sbjct: 1123 MCEASIGSPLVAEASAVRSAMCMAINLEFSSLEVLSDNLTLIRAISGITQAK-EIIGIVK 1182

Query: 74   DIRKIYGAFASITFQHVSRTHNFAAHSLVK 104
            DIR I   FASI+F H+SR+ N  A +L K
Sbjct: 1183 DIRSISTEFASISFAHISRSLNVEADNLAK 1211

[Close]

BLAST of HG10010036 vs. NCBI nr
Match: VDD60306.1 (unnamed protein product [Brassica oleracea])

HSP 1 Score: 57.4 bits (137), Expect = 1.1e-04
Identity = 37/90 (41.11%), Postives = 53/90 (58.89%), Query Frame = 0


Query: 14  VCRVSSLSSLCAEAYAIYENLHLVERLELSRLLVLSDSLSAVHMINGLEEAEVDGANTVM 73
           +C  S  S L AEA A+   + +   LE S L VLSD+L+ +  I+G+ +A+ +    V 
Sbjct: 98  MCEASIGSPLVAEASAVRSAMCMAINLEFSSLEVLSDNLTLIRAISGITQAK-EIIGIVK 157

Query: 74  DIRKIYGAFASITFQHVSRTHNFAAHSLVK 104
           DIR I   FASI+F H+SR+ N  A +L K
Sbjct: 158 DIRSISTEFASISFAHISRSLNVEADNLAK 186

[Close]

BLAST of HG10010036 vs. NCBI nr
Match: XP_013739634.1 (uncharacterized protein LOC106442499 [Brassica napus])

HSP 1 Score: 57.4 bits (137), Expect = 1.1e-04
Identity = 37/90 (41.11%), Postives = 53/90 (58.89%), Query Frame = 0


Query: 14  VCRVSSLSSLCAEAYAIYENLHLVERLELSRLLVLSDSLSAVHMINGLEEAEVDGANTVM 73
           +C  S  S L AEA A+   + +   LE S L VLSD+L+ +  I+G+ +A+ +    V 
Sbjct: 672 MCEASIGSPLVAEASAVRSAMCMAINLEFSSLEVLSDNLTLIRAISGITQAK-EIIGIVK 731

Query: 74  DIRKIYGAFASITFQHVSRTHNFAAHSLVK 104
           DIR I   FASI+F H+SR+ N  A +L K
Sbjct: 732 DIRSISTEFASISFAHISRSLNVEADNLAK 760

[Close]

BLAST of HG10010036 vs. NCBI nr
Match: XP_013703259.1 (uncharacterized protein LOC106407027 [Brassica napus])

HSP 1 Score: 57.4 bits (137), Expect = 1.1e-04
Identity = 37/90 (41.11%), Postives = 53/90 (58.89%), Query Frame = 0


Query: 14  VCRVSSLSSLCAEAYAIYENLHLVERLELSRLLVLSDSLSAVHMINGLEEAEVDGANTVM 73
           +C  S  S L AEA A+   + +   LE S L VLSD+L+ +  I+G+ +A+ +    V 
Sbjct: 300 MCEASIGSPLVAEASAVRSAMCMAINLEFSSLEVLSDNLTLIRAISGITQAK-EIIGIVK 359

Query: 74  DIRKIYGAFASITFQHVSRTHNFAAHSLVK 104
           DIR I   FASI+F H+SR+ N  A +L K
Sbjct: 360 DIRSISTEFASISFAHISRSLNVEADNLAK 388

[Close]

BLAST of HG10010036 vs. ExPASy TrEMBL
Match: A0A3P6GKF1 (RNase H domain-containing protein OS=Brassica oleracea OX=3712 GN=BOLC6T35759H PE=4 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 5.5e-05
Identity = 37/90 (41.11%), Postives = 53/90 (58.89%), Query Frame = 0


Query: 14  VCRVSSLSSLCAEAYAIYENLHLVERLELSRLLVLSDSLSAVHMINGLEEAEVDGANTVM 73
           +C  S  S L AEA A+   + +   LE S L VLSD+L+ +  I+G+ +A+ +    V 
Sbjct: 98  MCEASIGSPLVAEASAVRSAMCMAINLEFSSLEVLSDNLTLIRAISGITQAK-EIIGIVK 157

Query: 74  DIRKIYGAFASITFQHVSRTHNFAAHSLVK 104
           DIR I   FASI+F H+SR+ N  A +L K
Sbjct: 158 DIRSISTEFASISFAHISRSLNVEADNLAK 186

[Close]

BLAST of HG10010036 vs. ExPASy TrEMBL
Match: A0A3P6GLN9 (RNase H domain-containing protein OS=Brassica oleracea OX=3712 GN=BOLC8T50844H PE=4 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.7e-04
Identity = 37/84 (44.05%), Postives = 47/84 (55.95%), Query Frame = 0


Query: 21  SSLCAEAYAIYENLHLVERLELSRLLVLSDSLSAVHMI-NGLEEAEVDGANTVMDIRKIY 80
           S L AEA  I   L +   LE S L VLSDSL+    I + ++  E+ G   V DIR I 
Sbjct: 106 SPLIAEAITIQSTLSMALTLEFSTLKVLSDSLTLTRAISSNIQSKEIIG--IVKDIRAIS 165

Query: 81  GAFASITFQHVSRTHNFAAHSLVK 104
             FA+I+F HVS++ NF A  L K
Sbjct: 166 SGFATISFYHVSKSDNFVADGLAK 187

[Close]

BLAST of HG10010036 vs. TAIR 10
Match: AT1G10000.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 1.2e-04
Identity = 35/85 (41.18%), Postives = 47/85 (55.29%), Query Frame = 0


Query: 21  SSLCAEAYAIYENLHLVERLELSRLLVLSDSLSAVHMIN-GLEEAEVDGANTVMDIRKIY 80
           S L AEA+AI   +    +LE S LLVLSDS S V  +N  +   E+ G   +++IR I 
Sbjct: 203 SPLAAEAWAIKSAMLHALQLERSDLLVLSDSKSIVDALNSNVSLNEIFG--LLVEIRSIR 262

Query: 81  GAFASITFQHVSRTHNFAAHSLVKL 105
             F SI+FQ + R  N  A +  KL
Sbjct: 263 NRFRSISFQFIPRLVNSIADAAAKL 285

The following BLAST results are available for this feature:

BLAST of HG10010036 vs. NCBI nr


Match Name	E-value	Identity	Description
KAF7840958.1	3.9e-05	36.94	reverse transcriptase [Senna tora]	[more]
XP_013658645.1	1.1e-04	41.11	uncharacterized protein LOC106363457 [Brassica napus]	[more]
VDD60306.1	1.1e-04	41.11	unnamed protein product [Brassica oleracea]	[more]
XP_013739634.1	1.1e-04	41.11	uncharacterized protein LOC106442499 [Brassica napus]	[more]
XP_013703259.1	1.1e-04	41.11	uncharacterized protein LOC106407027 [Brassica napus]	[more]

BLAST of HG10010036 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of HG10010036 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A3P6GKF1	5.5e-05	41.11	RNase H domain-containing protein OS=Brassica oleracea OX=3712 GN=BOLC6T35759H P...	[more]
A0A3P6GLN9	2.7e-04	44.05	RNase H domain-containing protein OS=Brassica oleracea OX=3712 GN=BOLC8T50844H P...	[more]

BLAST of HG10010036 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G10000.1	1.2e-04	41.18	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 18..104 e-value: 9.8E-13 score: 48.0
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 13..111 e-value: 3.1E-10 score: 42.1
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 21..103 e-value: 4.56474E-10 score: 51.9312
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 20..108

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10010036.1	HG10010036.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity