HG10010032 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10010032
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Protein of unknown function (DUF803)
Location	Chr06: 17254975 .. 17259653 (-)
RNA-Seq Expression	HG10010032
Synteny	HG10010032

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGATTTCGTCTGATAATGTTCGTGGGTTTCTTCTTGCTGTTTCTTCCAGCGTCTTTATTGGGTCTAGTACTATTATCAAGAAGAAAGGTCTCATCAAATCAGGGACTGCTGGAACCAGAGCAGGTTTGGTTTGATCATCCTTCGGCTTTTTATTGTTAATGAAGAAAGCTATGAACAAAAATCTGAAAGGGTGAACTTCAAATATGAAATTGGATGATAACTTGTAAGTTCTATGAGTTTTTCTATGGTTATTAAATTATAAAGGCTCCATTTCATCATCATTTAGGTTTATTGGGCTCGTTTTCTCATCGTTTTCTCATCATTTCTTTACCATGTTTTTCATTTTTGTTAAAGAAAACTTTTGAATTCTTAGATTTTTTTTTTTTTTTTTTAGTTTTCAAAACTTGTATTGAATTTTGAAAGCATTTGTAAAATGTAGATAACATAATAATAATAAAATAAAATAAAATAAAAAATTGTAGGTGGGAATAGTGGTTATAACTTATAAGCTTAACTCTTAAAAACCAAAAACAAAAAATTAAGACTTTGTTTGGTAACCATTTGGTTTTTAGTTTTTGAAAATTAAGCCTATAAACACCTCTTTTACCTTCTTATTTTGTTATCTACTTCTTAGCAATGTTTTAAAAAACCAAGTCAAATTTTGAAAACTAAATAAAGTAGTTTCTAAAAACTTGTTTTTGTTTTTAGAATTTAGTTAAGAACTCGACTTTGATACTTAAGAAAAATTCAATTCATTATAAGAAAATGAGAGAAAATAGGCTTGATTTTCAAAAACAAAAAACGAAATGGTTACCAAATGAGACATAAATGGTTATCAAACGAGACTATTCTATCTATTCATTCTCTTCCAATACCTTATTGTGTTATCATGGTTTTCTATTTAAGCCAGTTCTACTGTCATGGTCCACACGTAGTTGCAATGGAAATTGGAACTCATGATTCTCTTCATATTTTCCCTCCTTCAATAGCTGTAGTTTAGTTCAATGTTATGCTAATTGGAAACAGACGTATTCTATCTTTTTCCAAACTCACATCAATTTATTACTTGTCTACATAAAAAGACTTGTATCTAAATATATTGTCCTAACAAATCTACAATATCCTACTTTGAGTTAATACAATCACATCTTGAAGGCTTGAACATCGGTCAAGTCTTATTCTTCTATTCATTTCAATAAAGTTCTTTTTTTTTTTTTTTTTTTTTTCCCTTTGTGTGTGGTCTTTATAGTGATTGTTGGGGAGACTGCTAACTTCGTTGCTTATGCATATGCTCCTGCGATTCTTGTCACTCCCTTGGGAGCTTTGAGCATTATTTTCAGGTATGTAACACAATAGGCTTGCAGACATTATGGAATGTCAGCTACGCGTAAATTCTTGAAGGTGGATTTTGAAAATTTAATCTGCCAGTGAAAAATTAGTGTGCAATTTGCTAACTGTAGCTTGTGATCTTCCATAATTGATGAATGAATAAAACAATCTCATGCAGTGCAGTTCTAGCTCACTTTATGTTGAAGGAAAAGTTGCATATCTTTGGAGTTCTTGGTTGTGTTCTCTGTGTTGTGGGTTCTACAACTATTGTTTTGCATGCTCCACTGGAAAAAAAGATTGAATCTGTTAAGGAAGTATGGCATCTTGCTACAGAGCCAGGTATAATATATTAGTGTTTCCTGAAGCTACGTAGTTTTGATCTTCCAAGATTTGGATGCTAATTTATGCTTCTTGCTCTTCTTCCAGGATTTCTGGTCTATTCATTCCTAGTAATAGTTGTAGTTGCAGTACTCATTTTTCGGTTTGCCCCACGCTATGGCCAGTCCCATATGATAATATATCTTGGAATTTGCTCTCTCATGGGATCTCTTACGGTTCGTTTGCTATCTTTGAAACTTGATAGCACGTCTTAATTTTACTTCATCTTTCTAAGGCTATGATAGTTTTCGTATTTTTCTTCAGGGTAGCTATTGCTGCCAAGTTGACATTGGAAGGAATGAATCAATTCAAGTACTTTGAGACATGGTTCTTCACTGTGTTTGTGATTGGCTGTTGTATTTTGCAGGTGATCTACTTGAACAAGGTAATATTTTGAAGTATTCAATTATATAAATATGTCTCCAACTTTCATAGATATACTCAATTCAACATAAGACTCACAACACACTAAGATATCTTCTGAAAACACACTGGCTCGAAACATTTTCAGTATCTCATCCTAAATAACACCCACCCTCCACAAAGAAGACTTTTCACCATCATTTGTAGAGATTTGTTGTTAAAATGATTTCTTTCCTTTCTAAATCCTAAATAATTCGATATAGCGAGTTAGGCTACAAAGATTTAAGGTCTATTTCTTCAATTTGTTTATACTTTCTAGAAAAATAAACCTTGTTGATTTCTAAGTTTTGAGTCTAGTTTTTATTTGGTCCCAAAAATTCCAAAATGTTATACTTACTATTGAGTTTTGTGTATAGTTTTCATTTGGTCTTAGGTTTCAAAGTGTTAAATTTTTACCTTTGAGTTTTGAGTTTTATTTCCATTTGGTCCCTAGGTTTCAAGATTCACACTTTTACTCTTTGATTTTTCACTAAATTATTTTAATTACTAGTAATTAATTCAAAATATTTAAATTAATTGTATTTGAATTAATTTTTAATAATTTTAAATCATTATTACTATTAATTAATAAATTTTACAATATAATTATTTTAAATTAATTTATAAGATAACACGAAAGTCAAAAGTGAGCATTAATGAAAATTGAAGGGTAATTGTGTAAAATATTGAAATTTAAGGACTACATAGAATTTAAGCTCAAAACTCAAGGATAAAAATATGACATTTTGAAATTTAGGGACCAAATGAATATTAAACTCAATATAAGGACTAAAAAGGTATTTTTCCGTAATTTCTAAGTTTGGCCTTCATTGTAGTTTCATGAAACTTGCTAGAACAGTGAACCTGGATTCAAGCACCCACACCCGTTGGGTTGACTTGGAAAATTAGAATTCAGGTCATCACAATTCACTTTTAACGTGGCACCAAAAGTAAAGGCTGAATTTATTTAATACACCAACTTTTAAGCATACTTTTCTTCAAATCACATGTGCCATGTGTTCTGTCATGAACTAAGAAGCTTGATGTTGAACACACGTCGAACACGCTCACGCTCACACTCTCGACATGTGTCCATCACGTAAAAAGGAGTATCTGATTAGTTAATTATTTTTAATGATAGATTAGGGCAGATTAAGTATGAGCTAGGGTTTAGGGAATCTAAATGGAATAAAAAGAAAAGAGCACACATACTTGTGATTCTATTTTCATGTCTAGAGAATCTAAATCTTGTTGGATGCTTTCTCACTTTTCTAATGGATAGTCTTTTTTCTTGTTGATTAAAATTATAGATTTCAAATTTATTTTTATCATATGAAATATGTTAAACATATTAGAATATGCACAAGTATATATATATATATATACCTTAAGAAACTAAGATTGTCCAATCATGTTACCGTCTTATTTTCTTTAGAAATTGACGCAAACACTTGGTTTGGTTAACACGTCCATATCTGATACATATTAGAAATTTGGACACTCCAACGCTTGTTGAACACTTGTTAGCACAATGGAGCGTGAGCCAAACAAACAATTATTAGGCGCATACCAATTAATATAATAAATAATCATAACTAATAGTATAGGACTAAATGAATGAATCTGAGTAAATGTGTTCTTGTTATCATATCCTAATTTTTCACAAAATGATGTGTATTCATGTCTCGTATGTGTATTCATGCTTCTTGGCAAGCCATCGTGTTTGTGTAGTGTGGTATTTGTCTGATGTGTCTGAGTTTGTGCTTTTTATGCATTTAATATATTTTGGCAATATTTCTTAAAATTTACCCGAGGAAATTTGATTTATAGTTTGATCACTATTTTCACAGGCTCTTGATGCTTTCAACTCCGTTGTTATATCTCCAGTTTACTATGTAACGTTCACAACTTTTACCATCCTTGCCAGTATGATTATGTTTAAGGTGAGTGAATTATGCTCTATGGGTTCAAGTAAAAGCATGATTCTAGAATTTTTATCTATTTTATTATTAAGTTCTTCAAAGCATCTTCTAATTTAAACTCCACATTTAGTCGTTGTCGGAAAATTTCTTCTTCCAAATTATTGAAAGTGAAACCATCATAGGTTGGCTTAATGGTCAATAAGGGTCACGTGTTTAATAAAAAGACATACGGATGAGTTCAATTCACAGTGACCATCTACTTAGGATTTAATATCCTACAAATTTTCTTAGTAGCTAAATGTTGTAGCATCAAGCGAGTTACTCCGGGAGATAAAACTCTATATCATACCTATGTCATGCAAAAAGCATGACGTCAAAGCTTTCAAGCAACGTTATGAACTGTGCCTGTGACTAAATATTCTCATTTTTTATTATTTTTTTATTTTTGGTTGCAGAATTGGGACACACAAAGTGCAACTCAAATTGCAACTGAAGTTTGTGGATTTATCACCATTCTTTCTGGGACCTTCCTTCTCCACAAAACCAAGGATATGGGAAAAGGTCCTTCCACTGAACCCCCTCTCTTCCAAACCCAAAACCATCCTCATGAAAATCAAAATCTAAATAGCACTAGAACACTATAG

mRNA sequence

ATGGTGATTTCGTCTGATAATGTTCGTGGGTTTCTTCTTGCTGTTTCTTCCAGCGTCTTTATTGGGTCTAGTACTATTATCAAGAAGAAAGGTCTCATCAAATCAGGGACTGCTGGAACCAGAGCAGTGATTGTTGGGGAGACTGCTAACTTCGTTGCTTATGCATATGCTCCTGCGATTCTTGTCACTCCCTTGGGAGCTTTGAGCATTATTTTCAGTGCAGTTCTAGCTCACTTTATGTTGAAGGAAAAGTTGCATATCTTTGGAGTTCTTGGTTGTGTTCTCTGTGTTGTGGGTTCTACAACTATTGTTTTGCATGCTCCACTGGAAAAAAAGATTGAATCTGTTAAGGAAGTATGGCATCTTGCTACAGAGCCAGGATTTCTGGTCTATTCATTCCTAGTAATAGTTGTAGTTGCAGTACTCATTTTTCGGTTTGCCCCACGCTATGGCCAGTCCCATATGATAATATATCTTGGAATTTGCTCTCTCATGGGATCTCTTACGGCTATGATAGTTTTCGTATTTTTCTTCAGGGTAGCTATTGCTGCCAAGTTGACATTGGAAGGAATGAATCAATTCAAGTACTTTGAGACATGGTTCTTCACTGTGTTTGTGATTGGCTGTTGTATTTTGCAGGTGATCTACTTGAACAAGGCTCTTGATGCTTTCAACTCCGTTGTTATATCTCCAGTTTACTATGTAACGTTCACAACTTTTACCATCCTTGCCAGTATGATTATGTTTAAGAATTGGGACACACAAAGTGCAACTCAAATTGCAACTGAAGTTTGTGGATTTATCACCATTCTTTCTGGGACCTTCCTTCTCCACAAAACCAAGGATATGGGAAAAGGTCCTTCCACTGAACCCCCTCTCTTCCAAACCCAAAACCATCCTCATGAAAATCAAAATCTAAATAGCACTAGAACACTATAG

Coding sequence (CDS)

ATGGTGATTTCGTCTGATAATGTTCGTGGGTTTCTTCTTGCTGTTTCTTCCAGCGTCTTTATTGGGTCTAGTACTATTATCAAGAAGAAAGGTCTCATCAAATCAGGGACTGCTGGAACCAGAGCAGTGATTGTTGGGGAGACTGCTAACTTCGTTGCTTATGCATATGCTCCTGCGATTCTTGTCACTCCCTTGGGAGCTTTGAGCATTATTTTCAGTGCAGTTCTAGCTCACTTTATGTTGAAGGAAAAGTTGCATATCTTTGGAGTTCTTGGTTGTGTTCTCTGTGTTGTGGGTTCTACAACTATTGTTTTGCATGCTCCACTGGAAAAAAAGATTGAATCTGTTAAGGAAGTATGGCATCTTGCTACAGAGCCAGGATTTCTGGTCTATTCATTCCTAGTAATAGTTGTAGTTGCAGTACTCATTTTTCGGTTTGCCCCACGCTATGGCCAGTCCCATATGATAATATATCTTGGAATTTGCTCTCTCATGGGATCTCTTACGGCTATGATAGTTTTCGTATTTTTCTTCAGGGTAGCTATTGCTGCCAAGTTGACATTGGAAGGAATGAATCAATTCAAGTACTTTGAGACATGGTTCTTCACTGTGTTTGTGATTGGCTGTTGTATTTTGCAGGTGATCTACTTGAACAAGGCTCTTGATGCTTTCAACTCCGTTGTTATATCTCCAGTTTACTATGTAACGTTCACAACTTTTACCATCCTTGCCAGTATGATTATGTTTAAGAATTGGGACACACAAAGTGCAACTCAAATTGCAACTGAAGTTTGTGGATTTATCACCATTCTTTCTGGGACCTTCCTTCTCCACAAAACCAAGGATATGGGAAAAGGTCCTTCCACTGAACCCCCTCTCTTCCAAACCCAAAACCATCCTCATGAAAATCAAAATCTAAATAGCACTAGAACACTATAG

Protein sequence

MVISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRAVIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGSTTIVLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGICSLMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKALDAFNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTKDMGKGPSTEPPLFQTQNHPHENQNLNSTRTL

Homology

[Close]

BLAST of HG10010032 vs. NCBI nr
Match: XP_038907003.1 (probable magnesium transporter NIPA2 isoform X1 [Benincasa hispida])

HSP 1 Score: 523.1 bits (1346), Expect = 1.7e-144
Identity = 285/331 (86.10%), Postives = 292/331 (88.22%), Query Frame = 0


Query: 1   MVISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA------------------ 60
           MVISSDNVRGFLLAVSSSVFIGSSTIIKKKGLI SG AGTRA                  
Sbjct: 1   MVISSDNVRGFLLAVSSSVFIGSSTIIKKKGLINSGAAGTRAASGGFSYLCEPWWWAGMI 60

Query: 61  -VIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGST 120
            + VGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGST
Sbjct: 61  TLSVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGST 120

Query: 121 TIVLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGI 180
           TIVLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVV  LIFR+AP YGQSHMIIY+GI
Sbjct: 121 TIVLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVVALIFRYAPLYGQSHMIIYVGI 180

Query: 181 CSLMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKAL 240
           CSL+GSLT M V      VAIAAKLT EGMNQFKYFETWFFTVFVIGCC LQVIYLNKAL
Sbjct: 181 CSLIGSLTVMSVKA----VAIAAKLTFEGMNQFKYFETWFFTVFVIGCCFLQVIYLNKAL 240

Query: 241 DAFNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTK 300
           DAFNS VISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTK
Sbjct: 241 DAFNSAVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTK 300

Query: 301 DMGKGPSTEPPLFQTQNHPHENQNLNSTRTL 313
           DMGKGP+TEPPLFQTQN+PH+NQN  S RTL
Sbjct: 301 DMGKGPATEPPLFQTQNYPHQNQNQYSGRTL 327

[Close]

BLAST of HG10010032 vs. NCBI nr
Match: XP_004138940.1 (probable magnesium transporter NIPA2 [Cucumis sativus] >XP_031736380.1 probable magnesium transporter NIPA2 [Cucumis sativus] >KGN61436.1 hypothetical protein Csa_006303 [Cucumis sativus])

HSP 1 Score: 517.3 bits (1331), Expect = 9.1e-143
Identity = 282/331 (85.20%), Postives = 294/331 (88.82%), Query Frame = 0


Query: 1   MVISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA------------------ 60
           M ISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSG AGTRA                  
Sbjct: 1   MGISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGAAGTRAASGGFSYLCEPWWWAGMI 60

Query: 61  -VIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGST 120
            +IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFG+LGCVLC+VGST
Sbjct: 61  TLIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGILGCVLCIVGST 120

Query: 121 TIVLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGI 180
           TIVLHAPLEKKIESVKEVWHLATEP FLVYSFLVIVVV VL+F++APRYGQSHMIIY+GI
Sbjct: 121 TIVLHAPLEKKIESVKEVWHLATEPVFLVYSFLVIVVVVVLVFKYAPRYGQSHMIIYVGI 180

Query: 181 CSLMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKAL 240
           CSLMGSLT M V      VAIAAKLT EGMNQFKYFETWFFTVFVIGCCILQVIYLNKAL
Sbjct: 181 CSLMGSLTVMSVKA----VAIAAKLTFEGMNQFKYFETWFFTVFVIGCCILQVIYLNKAL 240

Query: 241 DAFNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTK 300
           DAFNS VISPVYYV FTTFTILASMIMFK+WDTQSATQIATEVCGFITILSGTFLLHKTK
Sbjct: 241 DAFNSAVISPVYYVMFTTFTILASMIMFKDWDTQSATQIATEVCGFITILSGTFLLHKTK 300

Query: 301 DMGKGPSTEPPLFQTQNHPHENQNLNSTRTL 313
           DMGKGP T+PPLFQTQ+H H+NQNL+ TRTL
Sbjct: 301 DMGKGPPTQPPLFQTQSH-HQNQNLHPTRTL 326

[Close]

BLAST of HG10010032 vs. NCBI nr
Match: XP_008457147.1 (PREDICTED: probable magnesium transporter NIPA1 isoform X1 [Cucumis melo])

HSP 1 Score: 514.6 bits (1324), Expect = 5.9e-142
Identity = 277/331 (83.69%), Postives = 292/331 (88.22%), Query Frame = 0


Query: 1   MVISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA------------------ 60
           M ISSDNVRGFL AVSSSVFIGSSTIIKKKGLIKSG AGTRA                  
Sbjct: 1   MGISSDNVRGFLFAVSSSVFIGSSTIIKKKGLIKSGAAGTRAAYGGFSYLCEPWWWAGMI 60

Query: 61  -VIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGST 120
            +IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFG+LGCVLCVVGST
Sbjct: 61  TLIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGILGCVLCVVGST 120

Query: 121 TIVLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGI 180
           TIVLHAPLEKKIESVKEVWHLATEP FLVYSFLV+ VVAVLIF++APRYGQSHMIIY+GI
Sbjct: 121 TIVLHAPLEKKIESVKEVWHLATEPVFLVYSFLVMFVVAVLIFKYAPRYGQSHMIIYVGI 180

Query: 181 CSLMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKAL 240
           CSLMGSLT M V      VAIAAKLT EGMNQFKYFETWFF+VFV+GCCILQVIYLNKAL
Sbjct: 181 CSLMGSLTVMSVKA----VAIAAKLTFEGMNQFKYFETWFFSVFVVGCCILQVIYLNKAL 240

Query: 241 DAFNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTK 300
           DAFNS +ISP YYV FTTFTILA  IMFK+WDTQSATQIATEVCGFITILSGTFLLHKTK
Sbjct: 241 DAFNSAIISPFYYVMFTTFTILAGTIMFKDWDTQSATQIATEVCGFITILSGTFLLHKTK 300

Query: 301 DMGKGPSTEPPLFQTQNHPHENQNLNSTRTL 313
           DMGKGP+T+PPLFQTQ+HPH+NQNL +TRTL
Sbjct: 301 DMGKGPATQPPLFQTQSHPHQNQNLYTTRTL 327

[Close]

BLAST of HG10010032 vs. NCBI nr
Match: XP_022964060.1 (probable magnesium transporter NIPA1 isoform X1 [Cucurbita moschata] >XP_022964061.1 probable magnesium transporter NIPA1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 486.1 bits (1250), Expect = 2.3e-133
Identity = 265/328 (80.79%), Postives = 282/328 (85.98%), Query Frame = 0


Query: 1   MVISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA------------------ 60
           M I +DNVRGFLLAVSSSVFIGSSTIIKKKGLIKS  AGTRA                  
Sbjct: 1   MGILTDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSAAAGTRAASGGFSYLCEPWWWAGMI 60

Query: 61  -VIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGST 120
            +IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGC+LCVVGST
Sbjct: 61  TLIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCILCVVGST 120

Query: 121 TIVLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGI 180
           TIVLHAPL++KIESVKEVWHLAT+PGFLVYS L IVVVAVLIFRFAPRYG+SHMIIY+GI
Sbjct: 121 TIVLHAPLDRKIESVKEVWHLATQPGFLVYSCLTIVVVAVLIFRFAPRYGRSHMIIYIGI 180

Query: 181 CSLMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKAL 240
           CSLMGSLT M V      VAIA KLT  G+NQFKYFETWFFTVFVI  C+LQV+YLNKAL
Sbjct: 181 CSLMGSLTVMSVKA----VAIAVKLTFGGVNQFKYFETWFFTVFVIASCVLQVVYLNKAL 240

Query: 241 DAFNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTK 300
           DAFNS VISPVYYVTFTTFTI+ASMIMFKNWD+QSA QIATEVCGFITILSGTFLLHKTK
Sbjct: 241 DAFNSAVISPVYYVTFTTFTIIASMIMFKNWDSQSAAQIATEVCGFITILSGTFLLHKTK 300

Query: 301 DMGKGPSTEPPLFQTQNHPHENQNLNST 310
           DMGK P++EPPL QTQ   H +QNLNS+
Sbjct: 301 DMGKPPASEPPLIQTQTLSHPHQNLNSS 324

[Close]

BLAST of HG10010032 vs. NCBI nr
Match: KAG7026093.1 (putative magnesium transporter NIPA2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 486.1 bits (1250), Expect = 2.3e-133
Identity = 265/328 (80.79%), Postives = 282/328 (85.98%), Query Frame = 0


Query: 1   MVISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA------------------ 60
           M I +DNVRGFLLAVSSSVFIGSSTIIKKKGLIKS  AGTRA                  
Sbjct: 1   MGILTDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSAAAGTRAASGGFSYLCEPWWWAGMI 60

Query: 61  -VIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGST 120
            +IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGC+LCVVGST
Sbjct: 61  TLIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCILCVVGST 120

Query: 121 TIVLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGI 180
           TIVLHAPL++KIESVKEVWHLAT+PGFLVYS L IVVVAVLIFRFAPRYG+SHMIIY+GI
Sbjct: 121 TIVLHAPLDRKIESVKEVWHLATQPGFLVYSCLTIVVVAVLIFRFAPRYGRSHMIIYIGI 180

Query: 181 CSLMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKAL 240
           CSLMGSLT M V      VAIA KLT  G+NQFKYFETWFFTVFVI  C+LQV+YLNKAL
Sbjct: 181 CSLMGSLTVMSVKA----VAIAVKLTFGGVNQFKYFETWFFTVFVIASCVLQVVYLNKAL 240

Query: 241 DAFNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTK 300
           DAFNS VISPVYYVTFTTFTI+ASMIMFKNWD+QSA QIATEVCGFITILSGTFLLHKTK
Sbjct: 241 DAFNSAVISPVYYVTFTTFTIIASMIMFKNWDSQSAAQIATEVCGFITILSGTFLLHKTK 300

Query: 301 DMGKGPSTEPPLFQTQNHPHENQNLNST 310
           DMGK P++EPPL QTQ   H +QNLNS+
Sbjct: 301 DMGKPPTSEPPLIQTQTLSHPHQNLNSS 324

[Close]

BLAST of HG10010032 vs. ExPASy Swiss-Prot
Match: Q9LIR9 (Probable magnesium transporter NIPA1 OS=Arabidopsis thaliana OX=3702 GN=At3g23870 PE=2 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 2.0e-108
Identity = 209/305 (68.52%), Postives = 243/305 (79.67%), Query Frame = 0


Query: 3   ISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA-------------------V 62
           +S DN+ G +LAVSSS+FIGSS IIKKKGL K+G +G RA                   +
Sbjct: 4   MSPDNINGVILAVSSSIFIGSSFIIKKKGLKKAGASGVRAGEGGYGYLKEPWWWAGMITM 63

Query: 63  IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGSTTI 122
           IVGE ANF AYA+APAILVTPLGALSIIFSAVLAHF+LKEKLH+FG+LGC+LCVVGSTTI
Sbjct: 64  IVGEVANFAAYAFAPAILVTPLGALSIIFSAVLAHFILKEKLHMFGILGCILCVVGSTTI 123

Query: 123 VLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGICS 182
           VLHAP E+KIESVK++W LA EPGFLVYS ++++VVA+LIF + PRYG++HMI+Y+GICS
Sbjct: 124 VLHAPHEQKIESVKQIWQLAIEPGFLVYSAVIVIVVAILIFYYEPRYGKTHMIVYVGICS 183

Query: 183 LMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKALDA 242
           LMGSLT M V      VAIA KLT  G NQFKYF TW F + V  CCILQ+ YLNKALD 
Sbjct: 184 LMGSLTVMSVKA----VAIAIKLTFSGTNQFKYFNTWIFILVVATCCILQINYLNKALDT 243

Query: 243 FNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTKDM 289
           FN+ VISPVYYV FTTFTI+ASMIMFK+W +QS  +IATE+CGF+TILSGTFLLHKTKDM
Sbjct: 244 FNTAVISPVYYVMFTTFTIIASMIMFKDWASQSGLKIATELCGFVTILSGTFLLHKTKDM 303

[Close]

BLAST of HG10010032 vs. ExPASy Swiss-Prot
Match: B3LFA3 (Probable magnesium transporter NIPA2 OS=Arabidopsis thaliana OX=3702 GN=At4g13800 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 3.4e-108
Identity = 213/305 (69.84%), Postives = 241/305 (79.02%), Query Frame = 0


Query: 3   ISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA-------------------V 62
           +S DN+ G +LAVSSS+FIGSS IIKKKGL K+G +G RA                   +
Sbjct: 4   MSPDNIHGVILAVSSSIFIGSSFIIKKKGLKKAGVSGARAGEGGYGYLYEPWWWAGMITM 63

Query: 63  IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGSTTI 122
           IVGE ANF AYA+APAILVTPLGALSIIFSAVLAHF+L+EKLH+FG+LGCVLCVVGSTTI
Sbjct: 64  IVGEIANFAAYAFAPAILVTPLGALSIIFSAVLAHFILEEKLHMFGILGCVLCVVGSTTI 123

Query: 123 VLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGICS 182
           VLHAP E+ IESVK+VWHLATEPGFL YS +V+VVV  LIF + PRYG++HMI+Y+GICS
Sbjct: 124 VLHAPHEQGIESVKQVWHLATEPGFLAYSAVVLVVVLALIFYYEPRYGKTHMIVYVGICS 183

Query: 183 LMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKALDA 242
           LMGSLT M V      VAIA KLT  GMNQFKYF  W F + V  CCILQ+ YLNKALD 
Sbjct: 184 LMGSLTVMSVKA----VAIAIKLTFSGMNQFKYFHAWIFIIVVTICCILQINYLNKALDN 243

Query: 243 FNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTKDM 289
           FN+ VISPVYYV FTTFTILASMIMFK+W +QS  QIATE+CGF+TILSGTFLLHKTKDM
Sbjct: 244 FNTAVISPVYYVMFTTFTILASMIMFKDWASQSGLQIATELCGFVTILSGTFLLHKTKDM 303

[Close]

BLAST of HG10010032 vs. ExPASy Swiss-Prot
Match: Q94AH3 (Probable magnesium transporter NIPA4 OS=Arabidopsis thaliana OX=3702 GN=At1g71900 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.8e-94
Identity = 186/323 (57.59%), Postives = 241/323 (74.61%), Query Frame = 0


Query: 3   ISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA-------------------V 62
           +SSDN++G +LA+SSS+FIG+S I+KKKGL K+ + GTRA                   +
Sbjct: 15  MSSDNIKGLVLALSSSLFIGASFIVKKKGLKKAASTGTRAGVGGYSYLYEPLWWIGMTTM 74

Query: 63  IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGSTTI 122
           ++GE ANF AYA+APAILVTPLGA+SII SAVLAH +L+EKLHIFG+LGC LCVVGSTTI
Sbjct: 75  LLGEIANFAAYAFAPAILVTPLGAVSIIISAVLAHIILREKLHIFGILGCALCVVGSTTI 134

Query: 123 VLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGICS 182
           VLHAP E++I+SV EVW+LATEP F+ Y+ LVI     LI RF P+YGQ+++++Y+GICS
Sbjct: 135 VLHAPQEREIDSVIEVWNLATEPAFMFYASLVIGAAVFLIIRFVPQYGQTNVMVYIGICS 194

Query: 183 LMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKALDA 242
           L+GSL+ M V      + IA KLT  G NQ  Y +TW FT+ V+ C + Q+ YLNKALD 
Sbjct: 195 LVGSLSVMSVKA----LGIALKLTFSGTNQLFYPQTWIFTLVVLTCVVTQLNYLNKALDT 254

Query: 243 FNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTKDM 302
           FN+ ++SP+YYV FT+ TILAS+IMFK+WD Q+ TQI TE+CGF+TILSGTFLLH+TKDM
Sbjct: 255 FNTAIVSPIYYVMFTSLTILASVIMFKDWDRQNGTQIVTEICGFVTILSGTFLLHRTKDM 314

Query: 303 GKGPSTEPPLFQTQNHPHENQNL 307
            +G S   PL +   H +E + +
Sbjct: 315 VEGSSVILPL-RISKHINEEEGI 332

[Close]

BLAST of HG10010032 vs. ExPASy Swiss-Prot
Match: Q9LNK7 (Probable magnesium transporter NIPA3 OS=Arabidopsis thaliana OX=3702 GN=At1g34470 PE=2 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.4e-93
Identity = 184/306 (60.13%), Postives = 231/306 (75.49%), Query Frame = 0


Query: 3   ISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA-------------------V 62
           +SSDN++G +LA+SSS+FIG+S I+KKKGL ++G +G RA                   +
Sbjct: 15  MSSDNIKGLVLALSSSLFIGASFIVKKKGLKRAGASGLRAGSGGYSYLLEPLWWVGMITM 74

Query: 63  IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGSTTI 122
           IVGE ANF AYA+APAILVTPLGALSII SA LAH +L EKLH FG+LGCVLCVVGS TI
Sbjct: 75  IVGEIANFAAYAFAPAILVTPLGALSIIISAALAHVILHEKLHTFGLLGCVLCVVGSITI 134

Query: 123 VLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGICS 182
           VLHAP E++I+SV +VW+LATEP FL+Y+  V+    +LI +F P+YGQSH+++Y+G+CS
Sbjct: 135 VLHAPQEQEIDSVLQVWNLATEPAFLLYAAAVVGAAIILIVQFVPQYGQSHVMVYIGVCS 194

Query: 183 LMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKALDA 242
           L+GSL+ M V      + IA KLT  GMNQ  Y +TW FT+ V+ C I Q+ YLNKALD 
Sbjct: 195 LVGSLSVMSVKA----LGIALKLTFSGMNQLIYPQTWVFTLIVLTCVITQMNYLNKALDT 254

Query: 243 FNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTKDM 290
           FN+ V+SP+YYV FT+ TILAS+IMFK+WD Q  TQI TE+CGF+TILSGTFLLHKTKDM
Sbjct: 255 FNTAVVSPIYYVMFTSLTILASVIMFKDWDRQDGTQIVTELCGFVTILSGTFLLHKTKDM 314

[Close]

BLAST of HG10010032 vs. ExPASy Swiss-Prot
Match: F4JKQ7 (Probable magnesium transporter NIPA5 OS=Arabidopsis thaliana OX=3702 GN=At4g09640 PE=2 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 9.9e-92
Identity = 185/305 (60.66%), Postives = 224/305 (73.44%), Query Frame = 0


Query: 3   ISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA-------------------V 62
           +SSDNV+G +LA+SSS+FIG+S I+KKKGL K+G +G RA                   +
Sbjct: 15  MSSDNVKGLVLALSSSIFIGASFIVKKKGLKKAGASGLRAGSGGYSYLLEPLWWIGMITM 74

Query: 63  IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGSTTI 122
           IVGE ANF AYA+APAILVTPLGALSII SA LAH +L+EKLH FG+LGC LC+VGS TI
Sbjct: 75  IVGEIANFAAYAFAPAILVTPLGALSIIISASLAHIILQEKLHTFGILGCALCIVGSVTI 134

Query: 123 VLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGICS 182
           VLHAP E+ I SV EVW+LATEP FL Y+  V+    VLI +F P YGQSH+++Y+G+CS
Sbjct: 135 VLHAPQEQDIVSVLEVWNLATEPAFLFYAAAVVGAAIVLIVQFIPLYGQSHVMVYIGVCS 194

Query: 183 LMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKALDA 242
           L+GSL+ M V      + IA KLT  G NQ  Y +TW FTV V+ C I Q+ YLNKALD 
Sbjct: 195 LIGSLSVMSVKA----LGIALKLTFSGTNQLGYPQTWVFTVIVLFCVITQMNYLNKALDT 254

Query: 243 FNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTKDM 289
           FN+ V+SP+YYV FT+ TILAS+IMFK+WD QS TQI TE+CGF+TILSGTFLLH T DM
Sbjct: 255 FNTAVVSPIYYVMFTSLTILASVIMFKDWDRQSGTQIMTELCGFVTILSGTFLLHTTTDM 314

[Close]

BLAST of HG10010032 vs. ExPASy TrEMBL
Match: A0A0A0LN93 (Probable magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_2G122000 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 4.4e-143
Identity = 282/331 (85.20%), Postives = 294/331 (88.82%), Query Frame = 0


Query: 1   MVISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA------------------ 60
           M ISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSG AGTRA                  
Sbjct: 1   MGISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGAAGTRAASGGFSYLCEPWWWAGMI 60

Query: 61  -VIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGST 120
            +IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFG+LGCVLC+VGST
Sbjct: 61  TLIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGILGCVLCIVGST 120

Query: 121 TIVLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGI 180
           TIVLHAPLEKKIESVKEVWHLATEP FLVYSFLVIVVV VL+F++APRYGQSHMIIY+GI
Sbjct: 121 TIVLHAPLEKKIESVKEVWHLATEPVFLVYSFLVIVVVVVLVFKYAPRYGQSHMIIYVGI 180

Query: 181 CSLMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKAL 240
           CSLMGSLT M V      VAIAAKLT EGMNQFKYFETWFFTVFVIGCCILQVIYLNKAL
Sbjct: 181 CSLMGSLTVMSVKA----VAIAAKLTFEGMNQFKYFETWFFTVFVIGCCILQVIYLNKAL 240

Query: 241 DAFNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTK 300
           DAFNS VISPVYYV FTTFTILASMIMFK+WDTQSATQIATEVCGFITILSGTFLLHKTK
Sbjct: 241 DAFNSAVISPVYYVMFTTFTILASMIMFKDWDTQSATQIATEVCGFITILSGTFLLHKTK 300

Query: 301 DMGKGPSTEPPLFQTQNHPHENQNLNSTRTL 313
           DMGKGP T+PPLFQTQ+H H+NQNL+ TRTL
Sbjct: 301 DMGKGPPTQPPLFQTQSH-HQNQNLHPTRTL 326

[Close]

BLAST of HG10010032 vs. ExPASy TrEMBL
Match: A0A1S3C4E6 (Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103496891 PE=3 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 2.9e-142
Identity = 277/331 (83.69%), Postives = 292/331 (88.22%), Query Frame = 0


Query: 1   MVISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA------------------ 60
           M ISSDNVRGFL AVSSSVFIGSSTIIKKKGLIKSG AGTRA                  
Sbjct: 1   MGISSDNVRGFLFAVSSSVFIGSSTIIKKKGLIKSGAAGTRAAYGGFSYLCEPWWWAGMI 60

Query: 61  -VIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGST 120
            +IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFG+LGCVLCVVGST
Sbjct: 61  TLIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGILGCVLCVVGST 120

Query: 121 TIVLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGI 180
           TIVLHAPLEKKIESVKEVWHLATEP FLVYSFLV+ VVAVLIF++APRYGQSHMIIY+GI
Sbjct: 121 TIVLHAPLEKKIESVKEVWHLATEPVFLVYSFLVMFVVAVLIFKYAPRYGQSHMIIYVGI 180

Query: 181 CSLMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKAL 240
           CSLMGSLT M V      VAIAAKLT EGMNQFKYFETWFF+VFV+GCCILQVIYLNKAL
Sbjct: 181 CSLMGSLTVMSVKA----VAIAAKLTFEGMNQFKYFETWFFSVFVVGCCILQVIYLNKAL 240

Query: 241 DAFNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTK 300
           DAFNS +ISP YYV FTTFTILA  IMFK+WDTQSATQIATEVCGFITILSGTFLLHKTK
Sbjct: 241 DAFNSAIISPFYYVMFTTFTILAGTIMFKDWDTQSATQIATEVCGFITILSGTFLLHKTK 300

Query: 301 DMGKGPSTEPPLFQTQNHPHENQNLNSTRTL 313
           DMGKGP+T+PPLFQTQ+HPH+NQNL +TRTL
Sbjct: 301 DMGKGPATQPPLFQTQSHPHQNQNLYTTRTL 327

[Close]

BLAST of HG10010032 vs. ExPASy TrEMBL
Match: A0A6J1HJR4 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111464199 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.1e-133
Identity = 265/328 (80.79%), Postives = 282/328 (85.98%), Query Frame = 0


Query: 1   MVISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA------------------ 60
           M I +DNVRGFLLAVSSSVFIGSSTIIKKKGLIKS  AGTRA                  
Sbjct: 1   MGILTDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSAAAGTRAASGGFSYLCEPWWWAGMI 60

Query: 61  -VIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGST 120
            +IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGC+LCVVGST
Sbjct: 61  TLIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCILCVVGST 120

Query: 121 TIVLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGI 180
           TIVLHAPL++KIESVKEVWHLAT+PGFLVYS L IVVVAVLIFRFAPRYG+SHMIIY+GI
Sbjct: 121 TIVLHAPLDRKIESVKEVWHLATQPGFLVYSCLTIVVVAVLIFRFAPRYGRSHMIIYIGI 180

Query: 181 CSLMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKAL 240
           CSLMGSLT M V      VAIA KLT  G+NQFKYFETWFFTVFVI  C+LQV+YLNKAL
Sbjct: 181 CSLMGSLTVMSVKA----VAIAVKLTFGGVNQFKYFETWFFTVFVIASCVLQVVYLNKAL 240

Query: 241 DAFNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTK 300
           DAFNS VISPVYYVTFTTFTI+ASMIMFKNWD+QSA QIATEVCGFITILSGTFLLHKTK
Sbjct: 241 DAFNSAVISPVYYVTFTTFTIIASMIMFKNWDSQSAAQIATEVCGFITILSGTFLLHKTK 300

Query: 301 DMGKGPSTEPPLFQTQNHPHENQNLNST 310
           DMGK P++EPPL QTQ   H +QNLNS+
Sbjct: 301 DMGKPPASEPPLIQTQTLSHPHQNLNSS 324

[Close]

BLAST of HG10010032 vs. ExPASy TrEMBL
Match: A0A6J1KJ77 (Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111494491 PE=3 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.7e-132
Identity = 265/330 (80.30%), Postives = 282/330 (85.45%), Query Frame = 0


Query: 1   MVISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA------------------ 60
           M I +DNVRGFLLAVSSSVFIGSSTIIKKKGLIKS  AGTRA                  
Sbjct: 1   MGILTDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSAAAGTRAASGGFSYLCEPWWWAGMI 60

Query: 61  -VIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGST 120
            +IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGC+LCVVGST
Sbjct: 61  TLIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCILCVVGST 120

Query: 121 TIVLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGI 180
           TIVLHAPL++KIESVKEVWHLAT+PGFLVYS L IVVVAVLIFRFAPRYGQSHMIIY+GI
Sbjct: 121 TIVLHAPLDRKIESVKEVWHLATQPGFLVYSCLTIVVVAVLIFRFAPRYGQSHMIIYIGI 180

Query: 181 CSLMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKAL 240
           CSLMGSLT M V      VAIA KLT  G+NQFKYFETWFFTVFVI  C+LQV+YLNKAL
Sbjct: 181 CSLMGSLTVMSVKA----VAIAVKLTFGGVNQFKYFETWFFTVFVIASCVLQVVYLNKAL 240

Query: 241 DAFNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTK 300
           DAFNS VISPVYYVTFTTFTI+ASMIMFKNWD+QSA QI TEVCGFITILSGTFLLHKTK
Sbjct: 241 DAFNSAVISPVYYVTFTTFTIIASMIMFKNWDSQSAAQIGTEVCGFITILSGTFLLHKTK 300

Query: 301 DMGKGPSTEPPLFQ--TQNHPHENQNLNST 310
           DMGK P++EPPL Q  TQ+  H +QNLNS+
Sbjct: 301 DMGKPPASEPPLIQTHTQSLSHPHQNLNSS 326

[Close]

BLAST of HG10010032 vs. ExPASy TrEMBL
Match: A0A6J1CB86 (Probable magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111009915 PE=3 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 4.0e-128
Identity = 258/330 (78.18%), Postives = 277/330 (83.94%), Query Frame = 0


Query: 1   MVISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA------------------ 60
           M ISSDNVRGF+LAVSSSVFIGSSTIIKKKGLIKS + GTRA                  
Sbjct: 1   MGISSDNVRGFVLAVSSSVFIGSSTIIKKKGLIKSASTGTRAASGGFSYLREPWWWAGMI 60

Query: 61  -VIVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGST 120
            +I GE ANFVAYAYAPAILVTPLGALSIIFSAVLAHF+LKE LHIFGVLGCVLCVVGST
Sbjct: 61  TMICGEIANFVAYAYAPAILVTPLGALSIIFSAVLAHFILKENLHIFGVLGCVLCVVGST 120

Query: 121 TIVLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGI 180
           TIVLHAPLEK IESVKEVWHLATEPGFLVYS + IVVVAVLIF +APRYG SHM+IY+GI
Sbjct: 121 TIVLHAPLEKDIESVKEVWHLATEPGFLVYSSIAIVVVAVLIFWYAPRYGHSHMMIYVGI 180

Query: 181 CSLMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKAL 240
           CS+MGSLT M V      VAI+ KLT  GMNQFKYFETWFFTV VI  C+LQVIYLNKAL
Sbjct: 181 CSVMGSLTVMSV----KGVAISIKLTFSGMNQFKYFETWFFTVLVIVFCVLQVIYLNKAL 240

Query: 241 DAFNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTK 300
           DAFNS V+SPVYYVTFT FTI ASMIMFKNWD+QSA QIATEVCGFITILSGTFLLHKTK
Sbjct: 241 DAFNSAVVSPVYYVTFTAFTIFASMIMFKNWDSQSAAQIATEVCGFITILSGTFLLHKTK 300

Query: 301 DMGKGPSTEPPLFQT-QNHPHENQNLNSTR 311
           DMGK P+TEPP+FQ+ Q+ P ENQ+L S+R
Sbjct: 301 DMGKSPATEPPVFQSQQDQPRENQSLGSSR 326

[Close]

BLAST of HG10010032 vs. TAIR 10
Match: AT3G23870.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 393.7 bits (1010), Expect = 1.4e-109
Identity = 209/305 (68.52%), Postives = 243/305 (79.67%), Query Frame = 0


Query: 3   ISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA-------------------V 62
           +S DN+ G +LAVSSS+FIGSS IIKKKGL K+G +G RA                   +
Sbjct: 4   MSPDNINGVILAVSSSIFIGSSFIIKKKGLKKAGASGVRAGEGGYGYLKEPWWWAGMITM 63

Query: 63  IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGSTTI 122
           IVGE ANF AYA+APAILVTPLGALSIIFSAVLAHF+LKEKLH+FG+LGC+LCVVGSTTI
Sbjct: 64  IVGEVANFAAYAFAPAILVTPLGALSIIFSAVLAHFILKEKLHMFGILGCILCVVGSTTI 123

Query: 123 VLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGICS 182
           VLHAP E+KIESVK++W LA EPGFLVYS ++++VVA+LIF + PRYG++HMI+Y+GICS
Sbjct: 124 VLHAPHEQKIESVKQIWQLAIEPGFLVYSAVIVIVVAILIFYYEPRYGKTHMIVYVGICS 183

Query: 183 LMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKALDA 242
           LMGSLT M V      VAIA KLT  G NQFKYF TW F + V  CCILQ+ YLNKALD 
Sbjct: 184 LMGSLTVMSVKA----VAIAIKLTFSGTNQFKYFNTWIFILVVATCCILQINYLNKALDT 243

Query: 243 FNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTKDM 289
           FN+ VISPVYYV FTTFTI+ASMIMFK+W +QS  +IATE+CGF+TILSGTFLLHKTKDM
Sbjct: 244 FNTAVISPVYYVMFTTFTIIASMIMFKDWASQSGLKIATELCGFVTILSGTFLLHKTKDM 303

[Close]

BLAST of HG10010032 vs. TAIR 10
Match: AT4G13800.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 392.9 bits (1008), Expect = 2.4e-109
Identity = 213/305 (69.84%), Postives = 241/305 (79.02%), Query Frame = 0


Query: 3   ISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA-------------------V 62
           +S DN+ G +LAVSSS+FIGSS IIKKKGL K+G +G RA                   +
Sbjct: 4   MSPDNIHGVILAVSSSIFIGSSFIIKKKGLKKAGVSGARAGEGGYGYLYEPWWWAGMITM 63

Query: 63  IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGSTTI 122
           IVGE ANF AYA+APAILVTPLGALSIIFSAVLAHF+L+EKLH+FG+LGCVLCVVGSTTI
Sbjct: 64  IVGEIANFAAYAFAPAILVTPLGALSIIFSAVLAHFILEEKLHMFGILGCVLCVVGSTTI 123

Query: 123 VLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGICS 182
           VLHAP E+ IESVK+VWHLATEPGFL YS +V+VVV  LIF + PRYG++HMI+Y+GICS
Sbjct: 124 VLHAPHEQGIESVKQVWHLATEPGFLAYSAVVLVVVLALIFYYEPRYGKTHMIVYVGICS 183

Query: 183 LMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKALDA 242
           LMGSLT M V      VAIA KLT  GMNQFKYF  W F + V  CCILQ+ YLNKALD 
Sbjct: 184 LMGSLTVMSVKA----VAIAIKLTFSGMNQFKYFHAWIFIIVVTICCILQINYLNKALDN 243

Query: 243 FNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTKDM 289
           FN+ VISPVYYV FTTFTILASMIMFK+W +QS  QIATE+CGF+TILSGTFLLHKTKDM
Sbjct: 244 FNTAVISPVYYVMFTTFTILASMIMFKDWASQSGLQIATELCGFVTILSGTFLLHKTKDM 303

[Close]

BLAST of HG10010032 vs. TAIR 10
Match: AT1G71900.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 346.7 bits (888), Expect = 2.0e-95
Identity = 186/323 (57.59%), Postives = 241/323 (74.61%), Query Frame = 0


Query: 3   ISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA-------------------V 62
           +SSDN++G +LA+SSS+FIG+S I+KKKGL K+ + GTRA                   +
Sbjct: 15  MSSDNIKGLVLALSSSLFIGASFIVKKKGLKKAASTGTRAGVGGYSYLYEPLWWIGMTTM 74

Query: 63  IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGSTTI 122
           ++GE ANF AYA+APAILVTPLGA+SII SAVLAH +L+EKLHIFG+LGC LCVVGSTTI
Sbjct: 75  LLGEIANFAAYAFAPAILVTPLGAVSIIISAVLAHIILREKLHIFGILGCALCVVGSTTI 134

Query: 123 VLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGICS 182
           VLHAP E++I+SV EVW+LATEP F+ Y+ LVI     LI RF P+YGQ+++++Y+GICS
Sbjct: 135 VLHAPQEREIDSVIEVWNLATEPAFMFYASLVIGAAVFLIIRFVPQYGQTNVMVYIGICS 194

Query: 183 LMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKALDA 242
           L+GSL+ M V      + IA KLT  G NQ  Y +TW FT+ V+ C + Q+ YLNKALD 
Sbjct: 195 LVGSLSVMSVKA----LGIALKLTFSGTNQLFYPQTWIFTLVVLTCVVTQLNYLNKALDT 254

Query: 243 FNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTKDM 302
           FN+ ++SP+YYV FT+ TILAS+IMFK+WD Q+ TQI TE+CGF+TILSGTFLLH+TKDM
Sbjct: 255 FNTAIVSPIYYVMFTSLTILASVIMFKDWDRQNGTQIVTEICGFVTILSGTFLLHRTKDM 314

Query: 303 GKGPSTEPPLFQTQNHPHENQNL 307
            +G S   PL +   H +E + +
Sbjct: 315 VEGSSVILPL-RISKHINEEEGI 332

[Close]

BLAST of HG10010032 vs. TAIR 10
Match: AT1G34470.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 344.4 bits (882), Expect = 9.9e-95
Identity = 184/306 (60.13%), Postives = 231/306 (75.49%), Query Frame = 0


Query: 3   ISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA-------------------V 62
           +SSDN++G +LA+SSS+FIG+S I+KKKGL ++G +G RA                   +
Sbjct: 15  MSSDNIKGLVLALSSSLFIGASFIVKKKGLKRAGASGLRAGSGGYSYLLEPLWWVGMITM 74

Query: 63  IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGSTTI 122
           IVGE ANF AYA+APAILVTPLGALSII SA LAH +L EKLH FG+LGCVLCVVGS TI
Sbjct: 75  IVGEIANFAAYAFAPAILVTPLGALSIIISAALAHVILHEKLHTFGLLGCVLCVVGSITI 134

Query: 123 VLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGICS 182
           VLHAP E++I+SV +VW+LATEP FL+Y+  V+    +LI +F P+YGQSH+++Y+G+CS
Sbjct: 135 VLHAPQEQEIDSVLQVWNLATEPAFLLYAAAVVGAAIILIVQFVPQYGQSHVMVYIGVCS 194

Query: 183 LMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKALDA 242
           L+GSL+ M V      + IA KLT  GMNQ  Y +TW FT+ V+ C I Q+ YLNKALD 
Sbjct: 195 LVGSLSVMSVKA----LGIALKLTFSGMNQLIYPQTWVFTLIVLTCVITQMNYLNKALDT 254

Query: 243 FNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTKDM 290
           FN+ V+SP+YYV FT+ TILAS+IMFK+WD Q  TQI TE+CGF+TILSGTFLLHKTKDM
Sbjct: 255 FNTAVVSPIYYVMFTSLTILASVIMFKDWDRQDGTQIVTELCGFVTILSGTFLLHKTKDM 314

[Close]

BLAST of HG10010032 vs. TAIR 10
Match: AT4G09640.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 338.2 bits (866), Expect = 7.1e-93
Identity = 185/305 (60.66%), Postives = 224/305 (73.44%), Query Frame = 0


Query: 3   ISSDNVRGFLLAVSSSVFIGSSTIIKKKGLIKSGTAGTRA-------------------V 62
           +SSDNV+G +LA+SSS+FIG+S I+KKKGL K+G +G RA                   +
Sbjct: 15  MSSDNVKGLVLALSSSIFIGASFIVKKKGLKKAGASGLRAGSGGYSYLLEPLWWIGMITM 74

Query: 63  IVGETANFVAYAYAPAILVTPLGALSIIFSAVLAHFMLKEKLHIFGVLGCVLCVVGSTTI 122
           IVGE ANF AYA+APAILVTPLGALSII SA LAH +L+EKLH FG+LGC LC+VGS TI
Sbjct: 75  IVGEIANFAAYAFAPAILVTPLGALSIIISASLAHIILQEKLHTFGILGCALCIVGSVTI 134

Query: 123 VLHAPLEKKIESVKEVWHLATEPGFLVYSFLVIVVVAVLIFRFAPRYGQSHMIIYLGICS 182
           VLHAP E+ I SV EVW+LATEP FL Y+  V+    VLI +F P YGQSH+++Y+G+CS
Sbjct: 135 VLHAPQEQDIVSVLEVWNLATEPAFLFYAAAVVGAAIVLIVQFIPLYGQSHVMVYIGVCS 194

Query: 183 LMGSLTAMIVFVFFFRVAIAAKLTLEGMNQFKYFETWFFTVFVIGCCILQVIYLNKALDA 242
           L+GSL+ M V      + IA KLT  G NQ  Y +TW FTV V+ C I Q+ YLNKALD 
Sbjct: 195 LIGSLSVMSVKA----LGIALKLTFSGTNQLGYPQTWVFTVIVLFCVITQMNYLNKALDT 254

Query: 243 FNSVVISPVYYVTFTTFTILASMIMFKNWDTQSATQIATEVCGFITILSGTFLLHKTKDM 289
           FN+ V+SP+YYV FT+ TILAS+IMFK+WD QS TQI TE+CGF+TILSGTFLLH T DM
Sbjct: 255 FNTAVVSPIYYVMFTSLTILASVIMFKDWDRQSGTQIMTELCGFVTILSGTFLLHTTTDM 314

The following BLAST results are available for this feature:

BLAST of HG10010032 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038907003.1	1.7e-144	86.10	probable magnesium transporter NIPA2 isoform X1 [Benincasa hispida]	[more]
XP_004138940.1	9.1e-143	85.20	probable magnesium transporter NIPA2 [Cucumis sativus] >XP_031736380.1 probable ...	[more]
XP_008457147.1	5.9e-142	83.69	PREDICTED: probable magnesium transporter NIPA1 isoform X1 [Cucumis melo]	[more]
XP_022964060.1	2.3e-133	80.79	probable magnesium transporter NIPA1 isoform X1 [Cucurbita moschata] >XP_0229640...	[more]
KAG7026093.1	2.3e-133	80.79	putative magnesium transporter NIPA2 [Cucurbita argyrosperma subsp. argyrosperma...	[more]

BLAST of HG10010032 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LIR9	2.0e-108	68.52	Probable magnesium transporter NIPA1 OS=Arabidopsis thaliana OX=3702 GN=At3g2387...	[more]
B3LFA3	3.4e-108	69.84	Probable magnesium transporter NIPA2 OS=Arabidopsis thaliana OX=3702 GN=At4g1380...	[more]
Q94AH3	2.8e-94	57.59	Probable magnesium transporter NIPA4 OS=Arabidopsis thaliana OX=3702 GN=At1g7190...	[more]
Q9LNK7	1.4e-93	60.13	Probable magnesium transporter NIPA3 OS=Arabidopsis thaliana OX=3702 GN=At1g3447...	[more]
F4JKQ7	9.9e-92	60.66	Probable magnesium transporter NIPA5 OS=Arabidopsis thaliana OX=3702 GN=At4g0964...	[more]

BLAST of HG10010032 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LN93	4.4e-143	85.20	Probable magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_2G122000 PE=3 S...	[more]
A0A1S3C4E6	2.9e-142	83.69	Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103496891 PE=3 SV=1	[more]
A0A6J1HJR4	1.1e-133	80.79	Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111464199 PE=...	[more]
A0A6J1KJ77	2.7e-132	80.30	Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111494491 PE=3 ...	[more]
A0A6J1CB86	4.0e-128	78.18	Probable magnesium transporter OS=Momordica charantia OX=3673 GN=LOC111009915 PE...	[more]

BLAST of HG10010032 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23870.1	1.4e-109	68.52	Protein of unknown function (DUF803)	[more]
AT4G13800.1	2.4e-109	69.84	Protein of unknown function (DUF803)	[more]
AT1G71900.1	2.0e-95	57.59	Protein of unknown function (DUF803)	[more]
AT1G34470.1	9.9e-95	60.13	Protein of unknown function (DUF803)	[more]
AT4G09640.1	7.1e-93	60.66	Protein of unknown function (DUF803)	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008521	Magnesium transporter NIPA	PFAM	PF05653	Mg_trans_NIPA	coord: 38..283 e-value: 4.8E-100 score: 334.5
IPR008521	Magnesium transporter NIPA	PANTHER	PTHR12570	UNCHARACTERIZED	coord: 1..300
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 282..312
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 290..312
None	No IPR available	PANTHER	PTHR12570:SF20	MAGNESIUM TRANSPORTER NIPA1-RELATED	coord: 1..300
None	No IPR available	SUPERFAMILY	103481	Multidrug resistance efflux transporter EmrE	coord: 41..106

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10010032.1	HG10010032.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1903830	magnesium ion transmembrane transport
biological_process	GO:0015693	magnesium ion transport
cellular_component	GO:0005769	early endosome
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0015095	magnesium ion transmembrane transporter activity