HG10007276 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10007276
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionMechanosensitive ion channel protein
LocationChr10: 3278065 .. 3280753 (+)
RNA-Seq ExpressionHG10007276
SyntenyHG10007276
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGATAAGAAAGGAATGGAACAACTTGTTTTACGGATTTTGGAGGGTGAAGAAGGAGTTGATGCAAGTAAAGATCTCACTAAACCCTCAGTTGCTTCTTTTCCTGATTTTGAGCTAAAAGAAACTCGGAGTTTTAGGTGCACAATTCCACAATCTGCGGTTGGGAGTTCTCCTTCACATGAGATTTCCAGAATGACACCCCATAAACCTCCAAAAATTCCAGGCGAAACGGCAATTCGGCGCCCATCATTTGCTCGTTCGTCGTTTTCAAAGCCAAAATCAAGGCTAATAGAGTCACCTTGTCCTGATGGTGCATATTTAGCAGAAGAAAAGGCTCAAGCAAAATCAACATTCTATAGCTCTCCTAAGGTGGACTCCCCAGCTAAGATAACCGCCGTGACTAGTCCTAAAGAAGCTTTGAAGTCGGCCCCGATAACTCCGAAAACACCATTGATTGGAACTACTGGAAGTGAGGAGGAAGATGATGAAGAAGTTTACAAAACTGCAGAACTGAAAGTGAAAGAAAGATCAGGGAAGAAATTGAAAAGAACAGTTTTAATTGAATGGGTTGCATTTTTGTGCTTAACAGGGTGTTTAATTGCTAGCTTGACAATAGACAAGTTACTGACTAAAGAGATTTGGGGATTAGGACTGTGGAAATGGTGTGTTCTGGTATTAGTTATTTTCTGCGGTCGTTTGTTTTCGCAATGGTTTATCAACTGTCTGGTTTTCTTGATTGAAAGAAACTTTCTACTTAAAAGAAAGGTTCTTTATTTTGTCTATGGGCTGAGGAAGAGTGTTATAATTGTTATTTGGCTGGCTTTGGTTCTTCTAGCCTGGGGTCTATTATTTGATCAAAGCAGCAAGAGATCTAAAAAAGGCAATGAGATTCTGAATTATGTTACACGAGCTCTTGGTGCTTCTCTCATTGGAGCAGGATTATGGCTGGTGAAAACTTTGTTGGTGAAGATACTAGCTGCTTCTTTTCAATGCGCTAGATTCTTCGATCGGATTCAAGAATCAATCTTCCATCAATATATACTGCGCATCTTATCAGGACCTCCACTCATGGAGATGGCCGAGAGGGTCGGGAGAGCAGCAAGCACAGGGCAGTTGAGTTTCAGGCATTTGAAGAAAGAAAGTGATGGTGGGAATGAAGGGAAGGAAGAGGTGATTGATGTAGATAAACTCAAAAAGATGAAGCAAGAAAAAATCTCTGCTTGGACCATGAGAGGGCTAATCAATGTTATAAGGAGTTCAGGGCTGTCCACCATCTCTAATACAATAGAGAATTTTAAAGAGGAAGAGAATGAGCAAAAAGATAAGGAGATTAACAGTGAATGGGAAGCAAGGGCCGCAGCTTACCAGATTTTCAGAAATGTTGCGAAACCTGGCAGCAAGTGAGAATTATCCCTTCTGAAAGATCTTGAATTTCCCTCTTCATGGTATTTGGAGAATTTTGTTAACTAATGATTTTAATCTTTTGCCCATGGTTAGGTATATTGATGAAGAGGACCTCTTTCGTTTTATGAGTAAGGAGGAAATTGATAATGTGATGCCATTGTTTGAAGGAGGAGTTGAGACTGGCAGGATAAAGCGAAAAACCCTGAAGAATTGGCTGGTAAAATCTTTAGCATTTTCTTTTGAATACCTGAGTGTATTCAAGAGTTCAATTCTAAGCAAACCTCTTACAAACCAGGTCCTTAGAAATCTATTTTTGATGTTGCAGGTGAATGTTTACGTCGAACGCAAGTCGCTAGCTCACTCATTAAATGACACCAAGACTGCCATAGAGGAGCTAAACAAGCTTGCTTCTGCAATTGTACTGATTGTGATTATCATTGAATGGCTACTTTTGATGGGTTTCTTGACGACGCAAGTACTCGTCTTCTTTTCATCACAGCTTCTGTTGGTAGTTTTCATGTTCGGTAACACTGCCAGAACTGTATTCGAAGCCATCATATTCGTATTCGTGATGCATCCATTTGATGTGGGGGATCGTTGTGTCGTAGATGGTGTGCAGGTAACTTTCTCAACTCGGAATGTTTTTGGATTCTTTTGAAGCAACCTCACCTGGTTTAATTCTAACTTGCAGATGGTTGTTGAAGAAATGAACATTTTAACCACAATTTTCTTGAGATATGACAATGAGAAGATCTTCTATCCAAATTCTGTTCTAGCCACCAAACCCATCAGTAACTACTACAGGAGCCCCGAAATGAGCGATGCTATCGAATTTTCCGTTGACTTTTCCACTTCAATTGAAAGCATTGGAGCTCTAAAAGCAAGAATAAAAACGTAAGTTCCATTCAGTTTTTCTATGCTTGTTAGTAATAAACTACAAGGAAAGACCAAGCCTTATAATTCAAAGCATCCATTTGCTTGACACATAATTTCAAATGTATTACTCAGATACTTAGAAAGCAAACCTCAGTTCTGGCGTCCGAACCACAGTGTCGTCGTGAAGGAGATCGAGAATGTCAACAAGATGAAACTAGCTCTGTGTGTCAATCACACCATAAACTTTCAGAACTACGGCGACAAGAGCAGTCGCAGATCGGATTTAGTCTTGGAGCTGAAGAAAATTTTCGAAGATCTCGGGATCAAGTATCATCTGCTACCTCAAGAAGTTCATCTCAACTATGTGGGTTCAGCAGCTCCTGTGGTTCCATCCTCTCAAAGATGA

mRNA sequence

ATGGCGGATAAGAAAGGAATGGAACAACTTGTTTTACGGATTTTGGAGGGTGAAGAAGGAGTTGATGCAAGTAAAGATCTCACTAAACCCTCAGTTGCTTCTTTTCCTGATTTTGAGCTAAAAGAAACTCGGAGTTTTAGGTGCACAATTCCACAATCTGCGGTTGGGAGTTCTCCTTCACATGAGATTTCCAGAATGACACCCCATAAACCTCCAAAAATTCCAGGCGAAACGGCAATTCGGCGCCCATCATTTGCTCGTTCGTCGTTTTCAAAGCCAAAATCAAGGCTAATAGAGTCACCTTGTCCTGATGGTGCATATTTAGCAGAAGAAAAGGCTCAAGCAAAATCAACATTCTATAGCTCTCCTAAGGTGGACTCCCCAGCTAAGATAACCGCCGTGACTAGTCCTAAAGAAGCTTTGAAGTCGGCCCCGATAACTCCGAAAACACCATTGATTGGAACTACTGGAAGTGAGGAGGAAGATGATGAAGAAGTTTACAAAACTGCAGAACTGAAAGTGAAAGAAAGATCAGGGAAGAAATTGAAAAGAACAGTTTTAATTGAATGGGTTGCATTTTTGTGCTTAACAGGGTGTTTAATTGCTAGCTTGACAATAGACAAGTTACTGACTAAAGAGATTTGGGGATTAGGACTGTGGAAATGGTGTGTTCTGGTATTAGTTATTTTCTGCGGTCGTTTGTTTTCGCAATGGTTTATCAACTGTCTGGTTTTCTTGATTGAAAGAAACTTTCTACTTAAAAGAAAGGTTCTTTATTTTGTCTATGGGCTGAGGAAGAGTGTTATAATTGTTATTTGGCTGGCTTTGGTTCTTCTAGCCTGGGGTCTATTATTTGATCAAAGCAGCAAGAGATCTAAAAAAGGCAATGAGATTCTGAATTATGTTACACGAGCTCTTGGTGCTTCTCTCATTGGAGCAGGATTATGGCTGGTGAAAACTTTGTTGGTGAAGATACTAGCTGCTTCTTTTCAATGCGCTAGATTCTTCGATCGGATTCAAGAATCAATCTTCCATCAATATATACTGCGCATCTTATCAGGACCTCCACTCATGGAGATGGCCGAGAGGGTCGGGAGAGCAGCAAGCACAGGGCAGTTGAGTTTCAGGCATTTGAAGAAAGAAAGTGATGGTGGGAATGAAGGGAAGGAAGAGGTGATTGATGTAGATAAACTCAAAAAGATGAAGCAAGAAAAAATCTCTGCTTGGACCATGAGAGGGCTAATCAATGTTATAAGGAGTTCAGGGCTGTCCACCATCTCTAATACAATAGAGAATTTTAAAGAGGAAGAGAATGAGCAAAAAGATAAGGAGATTAACAGTGAATGGGAAGCAAGGGCCGCAGCTTACCAGATTTTCAGAAATGTTGCGAAACCTGGCAGCAAGTATATTGATGAAGAGGACCTCTTTCGTTTTATGAGTAAGGAGGAAATTGATAATGTGATGCCATTGTTTGAAGGAGGAGTTGAGACTGGCAGGATAAAGCGAAAAACCCTGAAGAATTGGCTGGTGAATGTTTACGTCGAACGCAAGTCGCTAGCTCACTCATTAAATGACACCAAGACTGCCATAGAGGAGCTAAACAAGCTTGCTTCTGCAATTGTACTGATTGTGATTATCATTGAATGGCTACTTTTGATGGGTTTCTTGACGACGCAAGTACTCGTCTTCTTTTCATCACAGCTTCTGTTGGTAGTTTTCATGTTCGGTAACACTGCCAGAACTGTATTCGAAGCCATCATATTCGTATTCGTGATGCATCCATTTGATGTGGGGGATCGTTGTGTCGTAGATGGTGTGCAGATGGTTGTTGAAGAAATGAACATTTTAACCACAATTTTCTTGAGATATGACAATGAGAAGATCTTCTATCCAAATTCTGTTCTAGCCACCAAACCCATCAGTAACTACTACAGGAGCCCCGAAATGAGCGATGCTATCGAATTTTCCGTTGACTTTTCCACTTCAATTGAAAGCATTGGAGCTCTAAAAGCAAGAATAAAAACATACTTAGAAAGCAAACCTCAGTTCTGGCGTCCGAACCACAGTGTCGTCGTGAAGGAGATCGAGAATGTCAACAAGATGAAACTAGCTCTGTGTGTCAATCACACCATAAACTTTCAGAACTACGGCGACAAGAGCAGTCGCAGATCGGATTTAGTCTTGGAGCTGAAGAAAATTTTCGAAGATCTCGGGATCAAGTATCATCTGCTACCTCAAGAAGTTCATCTCAACTATGTGGGTTCAGCAGCTCCTGTGGTTCCATCCTCTCAAAGATGA

Coding sequence (CDS)

ATGGCGGATAAGAAAGGAATGGAACAACTTGTTTTACGGATTTTGGAGGGTGAAGAAGGAGTTGATGCAAGTAAAGATCTCACTAAACCCTCAGTTGCTTCTTTTCCTGATTTTGAGCTAAAAGAAACTCGGAGTTTTAGGTGCACAATTCCACAATCTGCGGTTGGGAGTTCTCCTTCACATGAGATTTCCAGAATGACACCCCATAAACCTCCAAAAATTCCAGGCGAAACGGCAATTCGGCGCCCATCATTTGCTCGTTCGTCGTTTTCAAAGCCAAAATCAAGGCTAATAGAGTCACCTTGTCCTGATGGTGCATATTTAGCAGAAGAAAAGGCTCAAGCAAAATCAACATTCTATAGCTCTCCTAAGGTGGACTCCCCAGCTAAGATAACCGCCGTGACTAGTCCTAAAGAAGCTTTGAAGTCGGCCCCGATAACTCCGAAAACACCATTGATTGGAACTACTGGAAGTGAGGAGGAAGATGATGAAGAAGTTTACAAAACTGCAGAACTGAAAGTGAAAGAAAGATCAGGGAAGAAATTGAAAAGAACAGTTTTAATTGAATGGGTTGCATTTTTGTGCTTAACAGGGTGTTTAATTGCTAGCTTGACAATAGACAAGTTACTGACTAAAGAGATTTGGGGATTAGGACTGTGGAAATGGTGTGTTCTGGTATTAGTTATTTTCTGCGGTCGTTTGTTTTCGCAATGGTTTATCAACTGTCTGGTTTTCTTGATTGAAAGAAACTTTCTACTTAAAAGAAAGGTTCTTTATTTTGTCTATGGGCTGAGGAAGAGTGTTATAATTGTTATTTGGCTGGCTTTGGTTCTTCTAGCCTGGGGTCTATTATTTGATCAAAGCAGCAAGAGATCTAAAAAAGGCAATGAGATTCTGAATTATGTTACACGAGCTCTTGGTGCTTCTCTCATTGGAGCAGGATTATGGCTGGTGAAAACTTTGTTGGTGAAGATACTAGCTGCTTCTTTTCAATGCGCTAGATTCTTCGATCGGATTCAAGAATCAATCTTCCATCAATATATACTGCGCATCTTATCAGGACCTCCACTCATGGAGATGGCCGAGAGGGTCGGGAGAGCAGCAAGCACAGGGCAGTTGAGTTTCAGGCATTTGAAGAAAGAAAGTGATGGTGGGAATGAAGGGAAGGAAGAGGTGATTGATGTAGATAAACTCAAAAAGATGAAGCAAGAAAAAATCTCTGCTTGGACCATGAGAGGGCTAATCAATGTTATAAGGAGTTCAGGGCTGTCCACCATCTCTAATACAATAGAGAATTTTAAAGAGGAAGAGAATGAGCAAAAAGATAAGGAGATTAACAGTGAATGGGAAGCAAGGGCCGCAGCTTACCAGATTTTCAGAAATGTTGCGAAACCTGGCAGCAAGTATATTGATGAAGAGGACCTCTTTCGTTTTATGAGTAAGGAGGAAATTGATAATGTGATGCCATTGTTTGAAGGAGGAGTTGAGACTGGCAGGATAAAGCGAAAAACCCTGAAGAATTGGCTGGTGAATGTTTACGTCGAACGCAAGTCGCTAGCTCACTCATTAAATGACACCAAGACTGCCATAGAGGAGCTAAACAAGCTTGCTTCTGCAATTGTACTGATTGTGATTATCATTGAATGGCTACTTTTGATGGGTTTCTTGACGACGCAAGTACTCGTCTTCTTTTCATCACAGCTTCTGTTGGTAGTTTTCATGTTCGGTAACACTGCCAGAACTGTATTCGAAGCCATCATATTCGTATTCGTGATGCATCCATTTGATGTGGGGGATCGTTGTGTCGTAGATGGTGTGCAGATGGTTGTTGAAGAAATGAACATTTTAACCACAATTTTCTTGAGATATGACAATGAGAAGATCTTCTATCCAAATTCTGTTCTAGCCACCAAACCCATCAGTAACTACTACAGGAGCCCCGAAATGAGCGATGCTATCGAATTTTCCGTTGACTTTTCCACTTCAATTGAAAGCATTGGAGCTCTAAAAGCAAGAATAAAAACATACTTAGAAAGCAAACCTCAGTTCTGGCGTCCGAACCACAGTGTCGTCGTGAAGGAGATCGAGAATGTCAACAAGATGAAACTAGCTCTGTGTGTCAATCACACCATAAACTTTCAGAACTACGGCGACAAGAGCAGTCGCAGATCGGATTTAGTCTTGGAGCTGAAGAAAATTTTCGAAGATCTCGGGATCAAGTATCATCTGCTACCTCAAGAAGTTCATCTCAACTATGTGGGTTCAGCAGCTCCTGTGGTTCCATCCTCTCAAAGATGA

Protein sequence

MADKKGMEQLVLRILEGEEGVDASKDLTKPSVASFPDFELKETRSFRCTIPQSAVGSSPSHEISRMTPHKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDGAYLAEEKAQAKSTFYSSPKVDSPAKITAVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSGKKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIWLALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRILSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRSSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAIVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDAIEFSVDFSTSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHTINFQNYGDKSSRRSDLVLELKKIFEDLGIKYHLLPQEVHLNYVGSAAPVVPSSQR
Homology
BLAST of HG10007276 vs. NCBI nr
Match: XP_038878911.1 (mechanosensitive ion channel protein 10-like [Benincasa hispida])

HSP 1 Score: 1416.0 bits (3664), Expect = 0.0e+00
Identity = 733/765 (95.82%), Postives = 751/765 (98.17%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVDASKDLTKPSVASFPDFELKETRSFRCTIPQSAVGSSPS 60
           MADKKGMEQLVLRILEGEEGVD SKDLTKPSV+SFPDF+LKETRSFRCTIPQS VGSSPS
Sbjct: 1   MADKKGMEQLVLRILEGEEGVDTSKDLTKPSVSSFPDFDLKETRSFRCTIPQSVVGSSPS 60

Query: 61  HEISRMTPHKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDGAYLAEEKAQAKSTFY 120
           HEISRM+P KPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPD A LAEEKA+AKST Y
Sbjct: 61  HEISRMSPLKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDSASLAEEKAKAKSTLY 120

Query: 121 SSPKVDSPAKITAVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSGK 180
           SSPK+DSPAKIT VTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSGK
Sbjct: 121 SSPKMDSPAKITTVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSGK 180

Query: 181 KLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFSQWFI 240
           KLKRTVLIEWVAFLCLTGCLIASLTIDKL+TKEIWGLGLWKWCVLVLV FCGRLFSQWFI
Sbjct: 181 KLKRTVLIEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVSFCGRLFSQWFI 240

Query: 241 NCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIWLALVLLAWGLLFDQSSKRSKKGNEILN 300
           NCLVFLIERNFLLKRKVLYFVYGLRKSVII IWL LVLLAWGLLFDQSSKRSKKGNEILN
Sbjct: 241 NCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLGLVLLAWGLLFDQSSKRSKKGNEILN 300

Query: 301 YVTRALGASLIGAGLWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRILSGPPLMEM 360
           Y+TRALGASLIGAGLWLVKTL+VKILAASFQCARFFDRIQESIFHQYILRILSGPP+MEM
Sbjct: 301 YITRALGASLIGAGLWLVKTLMVKILAASFQCARFFDRIQESIFHQYILRILSGPPIMEM 360

Query: 361 AERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRS 420
           AE VGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRS
Sbjct: 361 AESVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRS 420

Query: 421 SGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMS 480
           SGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDE+DLFRFMS
Sbjct: 421 SGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRFMS 480

Query: 481 KEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAI 540
           KEEIDNV+PLFEGGVETG+IKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELNKLASAI
Sbjct: 481 KEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNKLASAI 540

Query: 541 VLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGDR 600
           VLIVIIIEWLLLMGFLTTQVLVF SSQ+LLVVFMFGNTARTVFEAIIFVFVMHPFDVGDR
Sbjct: 541 VLIVIIIEWLLLMGFLTTQVLVFVSSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGDR 600

Query: 601 CVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDAIEFSVDFS 660
           CVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSD+IEFSVDFS
Sbjct: 601 CVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIEFSVDFS 660

Query: 661 TSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHTINFQNYGDKS 720
           TSIESIGALKARIKTYLESKPQFWRPNHSV+VKEIEN+NKMKLALCVNHTINFQNYGDKS
Sbjct: 661 TSIESIGALKARIKTYLESKPQFWRPNHSVIVKEIENINKMKLALCVNHTINFQNYGDKS 720

Query: 721 SRRSDLVLELKKIFEDLGIKYHLLPQEVHLNYVGSAAPVVPSSQR 766
           SRRSDLVLELKKIFEDLGIKYHLLPQEV LNYVGSAA V+PSSQR
Sbjct: 721 SRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVGSAASVIPSSQR 765

BLAST of HG10007276 vs. NCBI nr
Match: XP_031738755.1 (mechanosensitive ion channel protein 10 [Cucumis sativus] >KGN66279.1 hypothetical protein Csa_007026 [Cucumis sativus])

HSP 1 Score: 1360.1 bits (3519), Expect = 0.0e+00
Identity = 704/759 (92.75%), Postives = 734/759 (96.71%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGV-DASKDLTKPSVASFPDFELKETRSFRCTIPQSAVGSSP 60
           MADKKGMEQLVLRILEGEEGV ++SKDL KPSV SFPDF+LKETRSFRCTIPQS VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDGAYLAEEKAQAKSTF 120
           SHEISRMTPHKPPKIPGET  RR SFA SSF+KPKSRLIE PCPDGA LAEEK  AKST 
Sbjct: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120

Query: 121 YSSPKVDSPAKITAVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSG 180
           YSS KVDSPAKIT VTSPKEALK+APITPKTPLIGTTG+EEEDDEEVY+TAELKVKE+SG
Sbjct: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180

Query: 181 KKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           K+LK+TV++EW+AFLCLTGCLIASLTI+ L+TKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGLRKSVII IWLALVLLAWGLLFDQSSKRSK+GN+IL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQC RFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MAERVGRAASTGQLSF+HLKKESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 SSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
            SGLSTISNTIENFKEEE E+KDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNV+PLFEGG ETG+IKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL+SA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540

Query: 541 IVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           ++LIVIIIEWLLLMGFLTTQVLVF SSQ+LLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDAIEFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSD+I+FSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPN+SVVVKEIENVNKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SSRRSDLVLELKKIFEDLGIKYHLLPQEVHLNYVGSAAP 759
           S+RRSDLVLELKKIFE+LGIKYHLLPQEV LNYV SAAP
Sbjct: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAAP 759

BLAST of HG10007276 vs. NCBI nr
Match: XP_008450905.1 (PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo] >XP_008450906.1 PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo] >TYK10044.1 mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 1352.0 bits (3498), Expect = 0.0e+00
Identity = 706/762 (92.65%), Postives = 729/762 (95.67%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGV-DASKDLTKPSVASFPDFELKETRSFRCTIPQSAVGSSP 60
           MADKKGMEQLVLRILEGEEGV  +SKDL KPSV SFPDF+LKETRSFRCTIP+S VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDGAYLAEEKAQAKSTF 120
           SHEISRMTP KPPKIPGET  RR SFA SSFSKPKSRLIE PCPDG  LAEEKA AKS+ 
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSPKVDSPAKITAVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSG 180
           Y SPKVDSPAKITAVTSPKEALK+ PITPKTPLIGTTG+EEEDDEEVYKTAELKVKE+S 
Sbjct: 121 YGSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           K+LK+TV++EWVAFLCLTGCLIASLTID L+TKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGL+KSVII IWLALVLLAWGLLFDQSSKRSKKGNEIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQC RFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSF+HLK+ESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 SSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
            SGLSTISNTIENFKEEE EQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNV+PLFEGG ETG+IKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 IVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           +VLIVIIIEWLLLMGFLTTQVLVF SSQ+LLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDAIEFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSD+I+FSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPN+SVVVKEIENVNKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SSRRSDLVLELKKIFEDLGIKYHLLPQEVHLNYVGSAAPVVP 762
           S+RRSDLVLELKKIFEDLGIKYHLLPQ V LNY  SAA  VP
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVP 762

BLAST of HG10007276 vs. NCBI nr
Match: KAA0055793.1 (mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 1349.7 bits (3492), Expect = 0.0e+00
Identity = 705/762 (92.52%), Postives = 729/762 (95.67%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGV-DASKDLTKPSVASFPDFELKETRSFRCTIPQSAVGSSP 60
           MADKKGMEQLVLRILEG EGV  +SKDL KPSV SFPDF+LKETRSFRCTIP+S VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGGEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDGAYLAEEKAQAKSTF 120
           SHEISRMTP KPPKIPGET  RR SFA SSFSKPKSRLIE PCPDG  LAEEKA AKS+ 
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSPKVDSPAKITAVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSG 180
           YSSPKVDSPAKITAVTSPKEALK+ PITPKTPLIGTTG+EEEDDEEVYKTAELKVKE+S 
Sbjct: 121 YSSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           K+LK+TV++EWVAFLCLTGCLIASLTID L+TKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGL+KSVII IWLALVLLAWGLLFDQSSKRSKKGNEIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQC RFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSF+HLK+ESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 SSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
            SGLSTISNTIENFKEEE EQKDKEINSEWEARAAAYQIFRNVAKPG+KYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGNKYIDEEDLFRFM 480

Query: 481 SKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNV+PLFEGG ETG+IKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 IVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           +VLIVIIIEWLLLMGFLTTQVLVF SSQ+LLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDAIEFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSD+I+FSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPN+SVVVKEIENVNKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SSRRSDLVLELKKIFEDLGIKYHLLPQEVHLNYVGSAAPVVP 762
           S+RRSDLVLELKKIFEDLGIKYHLLPQ V LNY  SAA  VP
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVP 762

BLAST of HG10007276 vs. NCBI nr
Match: XP_022987285.1 (mechanosensitive ion channel protein 10-like [Cucurbita maxima] >XP_022987286.1 mechanosensitive ion channel protein 10-like [Cucurbita maxima])

HSP 1 Score: 1317.4 bits (3408), Expect = 0.0e+00
Identity = 685/769 (89.08%), Postives = 720/769 (93.63%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVDASKDLTKPSVASFPDFELKETRSFRCTIPQSAVGSSPS 60
           MADKKG EQ+VLRIL+GEEGVDA   LT  SVASFPDFE KETRS RC IPQS V SSPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSPS 60

Query: 61  HEISRMTPHKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDGAYLAEEKAQAKST-- 120
           HEISRM+  KPPKIP E+A+RRPSFARSSFSKPKSRLIE PCPDGA  AEE AQAKST  
Sbjct: 61  HEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTSG 120

Query: 121 --FYSSPKVDSPAKITAVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKE 180
             F SSPK+DSPAKI   TSPKE+LKSAPITP+TPL+G+TGSEEEDDEEVYKTAELKVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGKKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240
           +S KKLKR VLIEW+AFLC+T CLI+SLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIWLALVLLAWGLLFDQSSKRSKKGN 300
           QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVI+ IWLALVLLAWGLLFDQS KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRILSGPP 360
           EILNYVTRAL ASLIGAGLWLVKTLLVKILAASFQC RFFDRIQESIFHQYILRILSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPP 360

Query: 361 LMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           LMEMAERVGR AS GQLSFRHLKKE DGGNEGKEEVIDVDKLKKMKQ K+SAWTMRGLIN
Sbjct: 361 LMEMAERVGRVASAGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENFKEEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMSKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFM+KEEIDNV+PLFEGGVETG+IKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+L
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASAIVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA VLIVIII WLLLMGFLTTQVLVF SSQLLLVVFMFGNTA+T+FEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDAIEFS 660
           VGDRCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISN+YRSPEMSD+I+FS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHTINFQNY 720
           VDFSTSIESIGALKARIK+YLESKPQFWRPNHSV+VKEIENVNKMKL+L +NHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDKSSRRSDLVLELKKIFEDLGIKYHLLPQEVHLNYVGSAAPVVPSSQR 766
           GD+SSRRSDLVLELKKIFEDLGIKYHLLPQEV LNYV S AP+V ++ R
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIAPMVSTTSR 769

BLAST of HG10007276 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 3.3e-197
Identity = 381/718 (53.06%), Postives = 507/718 (70.61%), Query Frame = 0

Query: 44  RSFRCTIPQSAVG----SSPSHEISRM--TPHKPPKIPGETAI---RRPSFARSSFSKPK 103
           RS     P+S  G     SPS EIS++  +P+KPP+ P +  +   +R SFARS +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 104 SRLIESPCP-DGAYLAEEKAQAKSTFYSSPKVDSPAKITAVTSPKEALKSAPITPKTPLI 163
           SR ++  CP D + L EE  +     +S  +     K     S +     AP+TP   ++
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNNK-----SNRSVGSPAPVTPSKVVV 147

Query: 164 GTTGSEEEDDEEVYKTAELKVKERSGKKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKE 223
                E+++DEE+YK  +L  + RS  K+    LIE   F+ +   L+ASLTI+ L    
Sbjct: 148 -----EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLKHHT 207

Query: 224 IWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIW 283
            WGL +WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVLYFV+GL+KSV + IW
Sbjct: 208 FWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIW 267

Query: 284 LALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCA 343
           L L+L+AW LLF+   KRS    ++L  +TR L + L GA  WLVKTLL+KILAA+F   
Sbjct: 268 LCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVN 327

Query: 344 RFFDRIQESIFHQYILRILSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVI 403
            FFDRIQ+S+FHQY+L+ LSG PLME AERVGR  STG LSF  + K+   G   +++VI
Sbjct: 328 NFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKKVI 387

Query: 404 DVDKLKKMKQEKISAWTMRGLINVIRSSGLSTISNTI-ENFKEEENEQKDKEINSEWEAR 463
           D+ K+ KMK+EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE EA 
Sbjct: 388 DMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEAL 447

Query: 464 AAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNV 523
           AAAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G  ETGRI RK    W+V V
Sbjct: 448 AAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKV 507

Query: 524 YVERKSLAHSLNDTKTAIEELNKLASAIVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVV 583
           Y  R++LAHSLNDTKTA+++LNKL +AI+++V ++ WLLL+   TT+VL+FFS+QL+ + 
Sbjct: 508 YTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALA 567

Query: 584 FMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPN 643
           F+ G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++YPN
Sbjct: 568 FIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPN 627

Query: 644 SVLATKPISNYYRSPEMSDAIEFSVDFSTSIESIGALKARIKTYLESKPQFWRPNHSVVV 703
           +VLATKPISNY+RSP M + +EFS+ FST +  I  LK RI  YLE  PQ W P HSVVV
Sbjct: 628 AVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVV 687

Query: 704 KEIENVNKMKLALCVNHTINFQNYGDKSSRRSDLVLELKKIFEDLGIKYHLLPQEVHL 751
           KEIEN+NK+K+AL  +HTI FQ   +++ RR++L L +K++ EDL I Y LLPQ+++L
Sbjct: 688 KEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of HG10007276 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 1.0e-174
Identity = 350/752 (46.54%), Postives = 494/752 (65.69%), Query Frame = 0

Query: 8   EQLVLRILEGEEGVDASKDLTKPSVASFPDFELKETRSFRCTIPQSAVGSSPSHEISRMT 67
           E++V+ + + E+  D     +   +AS PD +    +S    +P  ++ +   ++ S  +
Sbjct: 10  EEVVINVSDKEDSKDPRASPSFNPLAS-PDSDAGIEKS--KPVPPISIPTPEIYKFSG-S 69

Query: 68  PHKPPKIPG-ETAIRRPSFARSSFSKPKSRLIESPC--------PDGAYLAEEKAQAKST 127
            HKPPKIP  E  +RR S +RS +SKPKSR  E            +G     E+  A S 
Sbjct: 70  VHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQFGAGSF 129

Query: 128 FYSSPKVDSPAKITAVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERS 187
              S    SP             KS        L      E +++EE+YK  +L   +RS
Sbjct: 130 ARGSFDRASPNN-----------KSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRS 189

Query: 188 GKKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFSQW 247
           G  +K    +E V F+ + G LI SLTID +    IWGL  WKWCVLV+V   G L + W
Sbjct: 190 G--MKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNW 249

Query: 248 FINCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIWLALVLLAWGLLFDQSSKRSKKGNEI 307
           F++ +VF+IE+N+LL++KVLYFV+GL+K+V + IW +LVL+AW  LFD   KR++K    
Sbjct: 250 FMHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKTKRF 309

Query: 308 LNYVTRALGASLIGAGLWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRILSGPPLM 367
           L+++T  + + L+G+ L+LVKT  +K+LA+ F    FF+RIQES+FHQY+L+ LSGPPL+
Sbjct: 310 LDFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLI 369

Query: 368 EMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVI 427
           E AE VGR  STG LSF   K     G    ++VID+ K+ +MKQEK+SAWTMR LI  +
Sbjct: 370 EEAENVGRVPSTGHLSFTRTK----DGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAV 429

Query: 428 RSSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRF 487
            +SG+STIS+T++    ++ E+ DKEI +E EA AAAY +F NVAKP   YI+E+DL RF
Sbjct: 430 GTSGISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIEEDDLLRF 489

Query: 488 MSKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 547
           M KEE+D V+PL E   +TG+I RKT   W+VNVY  RK++ HSLNDTKTA+++L+KL +
Sbjct: 490 MIKEEVDLVLPLIE-DADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLIT 549

Query: 548 AIVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVG 607
            I+ ++  I W++L+   +T++L+ FSSQ L + FM G+T + +FE+ +FVFVMHP+DVG
Sbjct: 550 GILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVG 609

Query: 608 DRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDAIEFSVD 667
           DRCVVDGV ++VEE+++LTT+FL+ DNEK+FYPNSVL +KPISN+YRSP+M D ++F + 
Sbjct: 610 DRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIA 669

Query: 668 FSTSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHTINFQNYGD 727
           FST  E IG LK +I  YL +  Q W P   V+V+ IEN+NK+ L + V HTINFQ Y +
Sbjct: 670 FSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVE 729

Query: 728 KSSRRSDLVLELKKIFEDLGIKYHLLPQEVHL 751
           KS RR+ L++ +K+I EDL I Y LLPQ+V+L
Sbjct: 730 KSLRRTALIIAIKRILEDLEIDYTLLPQDVNL 738

BLAST of HG10007276 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 6.3e-148
Identity = 291/649 (44.84%), Postives = 421/649 (64.87%), Query Frame = 0

Query: 148 PKTPLIGTTGSEE-EDDEEVYKTAELKVKERSGKKLKRTVLIEWVAFLCLTGCLIASLTI 207
           P TP  G  GS++ E++E+ +   +L    R  +K+   V+IEW+  + +   LI SL I
Sbjct: 223 PGTPNQG--GSKDLEEEEDPFSEEDLPEGLRK-EKICVWVIIEWIFLILIIASLICSLVI 282

Query: 208 DKLLTKEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRK 267
             L  K +W L LWKW V+VLV+ CGRL S W +   V+ +E NFL ++KVLYFVYG+RK
Sbjct: 283 PYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRK 342

Query: 268 SVIIVIWLALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAGLWLVKTLLVKIL 327
            V   +WL LVL+AW  LFD+  +R  + + +L YVT+ L   L+   +WL+KTLLVK+L
Sbjct: 343 PVQNCLWLGLVLIAWHFLFDKKVEREMR-STVLKYVTKVLICLLVAVIIWLIKTLLVKVL 402

Query: 328 AASFQCARFFDRIQESIFHQYILRILSGPPLMEM---AERVGRAASTGQLSFRHLK---- 387
           A+SF  + +FDRIQES+F QY++  LSGPP +E+    E+V     T ++  R L     
Sbjct: 403 ASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGP 462

Query: 388 -----------------------------KESDGGNEGKEEVIDVDKLKKMKQEKISAWT 447
                                          S    EG EE I +D L++M  + +SAW 
Sbjct: 463 KAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWK 522

Query: 448 MRGLINVIRSSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYI 507
           M+ L+NVI+   LST+   I++   +E++ K  +I SE+EA+ AA +IF+NVA+PGS+YI
Sbjct: 523 MKKLMNVIKKGTLSTLDEQIQDTTTQEDD-KATQIRSEFEAKLAARKIFQNVAEPGSRYI 582

Query: 508 DEEDLFRFMSKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAI 567
             ED  RF+S++E +  M LFEG  E  +I +  LKNW+VN + ER++LA +LNDTKTA+
Sbjct: 583 YMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAV 642

Query: 568 EELNKLASAIVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVF 627
             L+++   +V IVI+I WLL++G  TT+ L+  SSQLLLVVF+FGN+ +T+FEA+IFVF
Sbjct: 643 NRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVF 702

Query: 628 VMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMS 687
           VMHPFDVGDRC +DGVQM+VEEMNILTT+FLR+DN+KI YPNS+L TKPI+NYYRSP+M 
Sbjct: 703 VMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQ 762

Query: 688 DAIEFSVDFSTSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHT 747
           DAIEF V  +T  E   AL+ RI +Y+++K   W P+  +V +++  +N +K+A+   H 
Sbjct: 763 DAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHK 822

Query: 748 INFQNYGDKSSRRSDLVLELKKIFEDLGIKYHLLPQEVHLNYVGSAAPV 760
           +N QN G++  RR  L+ E+ ++  +L I+Y L P  +++  + +A P+
Sbjct: 823 MNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATPI 866

BLAST of HG10007276 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 7.0e-147
Identity = 302/698 (43.27%), Postives = 438/698 (62.75%), Query Frame = 0

Query: 81  RRPSFARSSFSKPKSRLIESPCPDGAYLAEEKAQAKSTFYSSPKVDSPAKITAVTSPKEA 140
           ++P  +R   +K KSRL + P P   + A +K + KS   S                   
Sbjct: 185 KKPMLSR---NKTKSRLQDPPTP--THPAIDKTEMKSGRRSG-----------------I 244

Query: 141 LKSAPI--TPKTPLIGTTGSEEEDDEEVYKTAELKVKERSGKKLKRTVLIEWVAFLCLTG 200
            KS  +  +PK    G  G EEE++E+ +   +L  +E    KL   V +EW++ + +  
Sbjct: 245 FKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLP-EEFKRDKLSFWVFLEWISLVLIVT 304

Query: 201 CLIASLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVL 260
            L+ SLTI  L  K  W L LWKW V VLV+ CGRL S W +  +VFL+E+NF  +++VL
Sbjct: 305 SLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVL 364

Query: 261 YFVYGLRKSVIIVIWLALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAGLWLV 320
           YFVYG+RKSV   +WL LVLLAW  LFD+  +R  + +  L YVTR L   L+   +WLV
Sbjct: 365 YFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETR-STALRYVTRVLVCLLVALIIWLV 424

Query: 321 KTLLVKILAASFQCARFFDRIQESIFHQYILRILSGPPLMEM------------------ 380
           KT+LVK+LA+SF  + +FDRIQES+F QY++  LSGPPLME+                  
Sbjct: 425 KTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLE 484

Query: 381 ---AERVGRAASTGQLSFRHLKKESD----GGNEGKE-EVIDVDKLKKMKQEKISAWTMR 440
                ++  A      SF  + K       G   G++ E I +D+LK+M  + +SAW M+
Sbjct: 485 KLAGAKLPPALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMK 544

Query: 441 GLINVIRSSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDE 500
            L+N+I    +ST+   +++  +E+ +     I SE+EA+ AA +IF NV +PGS+YI  
Sbjct: 545 RLMNIILKGAISTLDQNMQDTTQEDEDA--THIRSEYEAKCAARKIFHNVTEPGSRYIYL 604

Query: 501 EDLFRFMSKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEE 560
           ED  RF+ +EE +  M LFEG  E+ +I +  LKNW+V  + ER++LA +LNDTKTA++ 
Sbjct: 605 EDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDR 664

Query: 561 LNKLASAIVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVFVM 620
           L+++ + ++ I+III WLL++G  TT+ L+  SSQLLLV F+FGN+ +T+FEAIIF+FVM
Sbjct: 665 LHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVM 724

Query: 621 HPFDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDA 680
           HPFDVGDRC +DGVQ+VVEEMNILTT+FLRYDN+KI YPNSVL TKPI+NYYRSP+M DA
Sbjct: 725 HPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDA 784

Query: 681 IEFSVDFSTSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHTIN 740
           +EF V  +T  E I A+K RI +Y+++K  +W P   +V   ++++N +K+A+ + H +N
Sbjct: 785 VEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMN 844

Query: 741 FQNYGDKSSRRSDLVLELKKIFEDLGIKYHLLPQEVHL 751
            Q+ G++  RR  L+ E+ K   +L I+Y L P  +++
Sbjct: 845 HQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINV 856

BLAST of HG10007276 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 5.5e-144
Identity = 294/684 (42.98%), Postives = 430/684 (62.87%), Query Frame = 0

Query: 112 KAQAKSTFYSSPKVDSPAKITAVTS---PKEALKSAPITPKTPLIGTTGSEEEDDEEVYK 171
           K + +S     P    P +   + S   PK     +    K+P   T G EEEDD     
Sbjct: 170 KMRTRSRLSDPPTPQLPPQTADMKSGRIPKSGQMKSGFFGKSP--KTQGEEEEDDP---F 229

Query: 172 TAELKVKERSGKKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKEIWGLGLWKWCVLVLV 231
            AE   +E    KL   +++EW++ + +    + +L I  L  K++W L LWKW  +VLV
Sbjct: 230 AAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLV 289

Query: 232 IFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIWLALVLLAWGLLFDQS 291
           + CGRL S W +  +VF IERNFLL+++VLYFVYG+RK+V   +WL LVLLAW  LFD+ 
Sbjct: 290 LICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDE- 349

Query: 292 SKRSKKGN-EILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCARFFDRIQESIFHQY 351
            K +K  N + L  VT+     L+G  LWLVKTLLVK+LA+SF  + +FDRIQES+F QY
Sbjct: 350 -KVAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQY 409

Query: 352 ILRILSGPPLMEM------AERVG--------------------RAASTGQLSFRHLKKE 411
           ++  LSGPPL+E+       ER+                         TG+  F      
Sbjct: 410 VIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGVEIQSGAQKSPMKTGKSPFLSHVLS 469

Query: 412 SDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRSSGLSTISNTIENFKEEENEQK 471
           + GG  G+ + I +D L K+  + +SAW M+ L+N+IR+  L+T+   +++     ++ K
Sbjct: 470 NGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQD--PSLDDDK 529

Query: 472 DKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVMPLFEGGVETGRIK 531
             +I SE+EA+ AA +IF NVAKPGSK+I   D+ RF+  +E    + LFEG  ET RI 
Sbjct: 530 GNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRIS 589

Query: 532 RKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAIVLIVIIIEWLLLMGFLTTQVL 591
           + +LKNW+VN + ER++LA +LNDTKTA+  L+K+ + +V I+I++ WL+++G  +T+ L
Sbjct: 590 KSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFL 649

Query: 592 VFFSSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILTTIFL 651
           V  SSQ+++V F+FGN  + VFE+II++FV+HPFDVGDRC +DGVQMVVEEMNILTT+FL
Sbjct: 650 VVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFL 709

Query: 652 RYDNEKIFYPNSVLATKPISNYYRSPEMSDAIEFSVDFSTSIESIGALKARIKTYLESKP 711
           R+DN+K+ YPNS+L TK I NYYRSP+M D IEFS+  +T  E I  +K RI +Y+E K 
Sbjct: 710 RFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKK 769

Query: 712 QFWRPNHSVVVKEIENVNKMKLALCVNHTINFQNYGDKSSRRSDLVLELKKIFEDLGIKY 766
             W P   +V K++E++N +++A+   H +N Q+ G+K +RRS LV E+ KI  +L I+Y
Sbjct: 770 DHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEY 829

BLAST of HG10007276 vs. ExPASy TrEMBL
Match: A0A0A0LYZ9 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_1G595830 PE=3 SV=1)

HSP 1 Score: 1360.1 bits (3519), Expect = 0.0e+00
Identity = 704/759 (92.75%), Postives = 734/759 (96.71%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGV-DASKDLTKPSVASFPDFELKETRSFRCTIPQSAVGSSP 60
           MADKKGMEQLVLRILEGEEGV ++SKDL KPSV SFPDF+LKETRSFRCTIPQS VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDGAYLAEEKAQAKSTF 120
           SHEISRMTPHKPPKIPGET  RR SFA SSF+KPKSRLIE PCPDGA LAEEK  AKST 
Sbjct: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120

Query: 121 YSSPKVDSPAKITAVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSG 180
           YSS KVDSPAKIT VTSPKEALK+APITPKTPLIGTTG+EEEDDEEVY+TAELKVKE+SG
Sbjct: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180

Query: 181 KKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           K+LK+TV++EW+AFLCLTGCLIASLTI+ L+TKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGLRKSVII IWLALVLLAWGLLFDQSSKRSK+GN+IL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQC RFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MAERVGRAASTGQLSF+HLKKESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 SSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
            SGLSTISNTIENFKEEE E+KDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNV+PLFEGG ETG+IKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL+SA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540

Query: 541 IVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           ++LIVIIIEWLLLMGFLTTQVLVF SSQ+LLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDAIEFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSD+I+FSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPN+SVVVKEIENVNKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SSRRSDLVLELKKIFEDLGIKYHLLPQEVHLNYVGSAAP 759
           S+RRSDLVLELKKIFE+LGIKYHLLPQEV LNYV SAAP
Sbjct: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAAP 759

BLAST of HG10007276 vs. ExPASy TrEMBL
Match: A0A1S3BR08 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103492355 PE=3 SV=1)

HSP 1 Score: 1352.0 bits (3498), Expect = 0.0e+00
Identity = 706/762 (92.65%), Postives = 729/762 (95.67%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGV-DASKDLTKPSVASFPDFELKETRSFRCTIPQSAVGSSP 60
           MADKKGMEQLVLRILEGEEGV  +SKDL KPSV SFPDF+LKETRSFRCTIP+S VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDGAYLAEEKAQAKSTF 120
           SHEISRMTP KPPKIPGET  RR SFA SSFSKPKSRLIE PCPDG  LAEEKA AKS+ 
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSPKVDSPAKITAVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSG 180
           Y SPKVDSPAKITAVTSPKEALK+ PITPKTPLIGTTG+EEEDDEEVYKTAELKVKE+S 
Sbjct: 121 YGSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           K+LK+TV++EWVAFLCLTGCLIASLTID L+TKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGL+KSVII IWLALVLLAWGLLFDQSSKRSKKGNEIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQC RFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSF+HLK+ESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 SSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
            SGLSTISNTIENFKEEE EQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNV+PLFEGG ETG+IKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 IVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           +VLIVIIIEWLLLMGFLTTQVLVF SSQ+LLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDAIEFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSD+I+FSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPN+SVVVKEIENVNKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SSRRSDLVLELKKIFEDLGIKYHLLPQEVHLNYVGSAAPVVP 762
           S+RRSDLVLELKKIFEDLGIKYHLLPQ V LNY  SAA  VP
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVP 762

BLAST of HG10007276 vs. ExPASy TrEMBL
Match: A0A5D3CFQ7 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001770 PE=3 SV=1)

HSP 1 Score: 1352.0 bits (3498), Expect = 0.0e+00
Identity = 706/762 (92.65%), Postives = 729/762 (95.67%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGV-DASKDLTKPSVASFPDFELKETRSFRCTIPQSAVGSSP 60
           MADKKGMEQLVLRILEGEEGV  +SKDL KPSV SFPDF+LKETRSFRCTIP+S VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDGAYLAEEKAQAKSTF 120
           SHEISRMTP KPPKIPGET  RR SFA SSFSKPKSRLIE PCPDG  LAEEKA AKS+ 
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSPKVDSPAKITAVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSG 180
           Y SPKVDSPAKITAVTSPKEALK+ PITPKTPLIGTTG+EEEDDEEVYKTAELKVKE+S 
Sbjct: 121 YGSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           K+LK+TV++EWVAFLCLTGCLIASLTID L+TKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGL+KSVII IWLALVLLAWGLLFDQSSKRSKKGNEIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQC RFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSF+HLK+ESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 SSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
            SGLSTISNTIENFKEEE EQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNV+PLFEGG ETG+IKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 IVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           +VLIVIIIEWLLLMGFLTTQVLVF SSQ+LLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDAIEFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSD+I+FSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPN+SVVVKEIENVNKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SSRRSDLVLELKKIFEDLGIKYHLLPQEVHLNYVGSAAPVVP 762
           S+RRSDLVLELKKIFEDLGIKYHLLPQ V LNY  SAA  VP
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVP 762

BLAST of HG10007276 vs. ExPASy TrEMBL
Match: A0A5A7UIR3 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001820 PE=3 SV=1)

HSP 1 Score: 1349.7 bits (3492), Expect = 0.0e+00
Identity = 705/762 (92.52%), Postives = 729/762 (95.67%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGV-DASKDLTKPSVASFPDFELKETRSFRCTIPQSAVGSSP 60
           MADKKGMEQLVLRILEG EGV  +SKDL KPSV SFPDF+LKETRSFRCTIP+S VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGGEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPHKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDGAYLAEEKAQAKSTF 120
           SHEISRMTP KPPKIPGET  RR SFA SSFSKPKSRLIE PCPDG  LAEEKA AKS+ 
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSPKVDSPAKITAVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSG 180
           YSSPKVDSPAKITAVTSPKEALK+ PITPKTPLIGTTG+EEEDDEEVYKTAELKVKE+S 
Sbjct: 121 YSSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           K+LK+TV++EWVAFLCLTGCLIASLTID L+TKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGL+KSVII IWLALVLLAWGLLFDQSSKRSKKGNEIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQC RFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSF+HLK+ESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 SSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
            SGLSTISNTIENFKEEE EQKDKEINSEWEARAAAYQIFRNVAKPG+KYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGNKYIDEEDLFRFM 480

Query: 481 SKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNV+PLFEGG ETG+IKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 IVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           +VLIVIIIEWLLLMGFLTTQVLVF SSQ+LLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDAIEFSVDF 660
           RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSD+I+FSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPN+SVVVKEIENVNKMKLALCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SSRRSDLVLELKKIFEDLGIKYHLLPQEVHLNYVGSAAPVVP 762
           S+RRSDLVLELKKIFEDLGIKYHLLPQ V LNY  SAA  VP
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVP 762

BLAST of HG10007276 vs. ExPASy TrEMBL
Match: A0A6J1JDR1 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111484882 PE=3 SV=1)

HSP 1 Score: 1317.4 bits (3408), Expect = 0.0e+00
Identity = 685/769 (89.08%), Postives = 720/769 (93.63%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVDASKDLTKPSVASFPDFELKETRSFRCTIPQSAVGSSPS 60
           MADKKG EQ+VLRIL+GEEGVDA   LT  SVASFPDFE KETRS RC IPQS V SSPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSPS 60

Query: 61  HEISRMTPHKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDGAYLAEEKAQAKST-- 120
           HEISRM+  KPPKIP E+A+RRPSFARSSFSKPKSRLIE PCPDGA  AEE AQAKST  
Sbjct: 61  HEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTSG 120

Query: 121 --FYSSPKVDSPAKITAVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKE 180
             F SSPK+DSPAKI   TSPKE+LKSAPITP+TPL+G+TGSEEEDDEEVYKTAELKVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSGKKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240
           +S KKLKR VLIEW+AFLC+T CLI+SLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIWLALVLLAWGLLFDQSSKRSKKGN 300
           QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVI+ IWLALVLLAWGLLFDQS KRSKK N
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRILSGPP 360
           EILNYVTRAL ASLIGAGLWLVKTLLVKILAASFQC RFFDRIQESIFHQYILRILSGPP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPP 360

Query: 361 LMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLIN 420
           LMEMAERVGR AS GQLSFRHLKKE DGGNEGKEEVIDVDKLKKMKQ K+SAWTMRGLIN
Sbjct: 361 LMEMAERVGRVASAGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLIN 420

Query: 421 VIRSSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLF 480
           VIRSSGLSTISNTIENFKEEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 RFMSKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL 540
           RFM+KEEIDNV+PLFEGGVETG+IKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+L
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASAIVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVFVMHPFD 600
           ASA VLIVIII WLLLMGFLTTQVLVF SSQLLLVVFMFGNTA+T+FEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDAIEFS 660
           VGDRCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISN+YRSPEMSD+I+FS
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDFS 660

Query: 661 VDFSTSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHTINFQNY 720
           VDFSTSIESIGALKARIK+YLESKPQFWRPNHSV+VKEIENVNKMKL+L +NHTINFQNY
Sbjct: 661 VDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQNY 720

Query: 721 GDKSSRRSDLVLELKKIFEDLGIKYHLLPQEVHLNYVGSAAPVVPSSQR 766
           GD+SSRRSDLVLELKKIFEDLGIKYHLLPQEV LNYV S AP+V ++ R
Sbjct: 721 GDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIAPMVSTTSR 769

BLAST of HG10007276 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 689.9 bits (1779), Expect = 2.3e-198
Identity = 381/718 (53.06%), Postives = 507/718 (70.61%), Query Frame = 0

Query: 44  RSFRCTIPQSAVG----SSPSHEISRM--TPHKPPKIPGETAI---RRPSFARSSFSKPK 103
           RS     P+S  G     SPS EIS++  +P+KPP+ P +  +   +R SFARS +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 104 SRLIESPCP-DGAYLAEEKAQAKSTFYSSPKVDSPAKITAVTSPKEALKSAPITPKTPLI 163
           SR ++  CP D + L EE  +     +S  +     K     S +     AP+TP   ++
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNNK-----SNRSVGSPAPVTPSKVVV 147

Query: 164 GTTGSEEEDDEEVYKTAELKVKERSGKKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKE 223
                E+++DEE+YK  +L  + RS  K+    LIE   F+ +   L+ASLTI+ L    
Sbjct: 148 -----EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLKHHT 207

Query: 224 IWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIW 283
            WGL +WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVLYFV+GL+KSV + IW
Sbjct: 208 FWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIW 267

Query: 284 LALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCA 343
           L L+L+AW LLF+   KRS    ++L  +TR L + L GA  WLVKTLL+KILAA+F   
Sbjct: 268 LCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVN 327

Query: 344 RFFDRIQESIFHQYILRILSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVI 403
            FFDRIQ+S+FHQY+L+ LSG PLME AERVGR  STG LSF  + K+   G   +++VI
Sbjct: 328 NFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKKVI 387

Query: 404 DVDKLKKMKQEKISAWTMRGLINVIRSSGLSTISNTI-ENFKEEENEQKDKEINSEWEAR 463
           D+ K+ KMK+EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE EA 
Sbjct: 388 DMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEAL 447

Query: 464 AAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNV 523
           AAAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G  ETGRI RK    W+V V
Sbjct: 448 AAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKV 507

Query: 524 YVERKSLAHSLNDTKTAIEELNKLASAIVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVV 583
           Y  R++LAHSLNDTKTA+++LNKL +AI+++V ++ WLLL+   TT+VL+FFS+QL+ + 
Sbjct: 508 YTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALA 567

Query: 584 FMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPN 643
           F+ G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++YPN
Sbjct: 568 FIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPN 627

Query: 644 SVLATKPISNYYRSPEMSDAIEFSVDFSTSIESIGALKARIKTYLESKPQFWRPNHSVVV 703
           +VLATKPISNY+RSP M + +EFS+ FST +  I  LK RI  YLE  PQ W P HSVVV
Sbjct: 628 AVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVV 687

Query: 704 KEIENVNKMKLALCVNHTINFQNYGDKSSRRSDLVLELKKIFEDLGIKYHLLPQEVHL 751
           KEIEN+NK+K+AL  +HTI FQ   +++ RR++L L +K++ EDL I Y LLPQ+++L
Sbjct: 688 KEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of HG10007276 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 689.9 bits (1779), Expect = 2.3e-198
Identity = 381/718 (53.06%), Postives = 507/718 (70.61%), Query Frame = 0

Query: 44  RSFRCTIPQSAVG----SSPSHEISRM--TPHKPPKIPGETAI---RRPSFARSSFSKPK 103
           RS     P+S  G     SPS EIS++  +P+KPP+ P +  +   +R SFARS +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 104 SRLIESPCP-DGAYLAEEKAQAKSTFYSSPKVDSPAKITAVTSPKEALKSAPITPKTPLI 163
           SR ++  CP D + L EE  +     +S  +     K     S +     AP+TP   ++
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNNK-----SNRSVGSPAPVTPSKVVV 147

Query: 164 GTTGSEEEDDEEVYKTAELKVKERSGKKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKE 223
                E+++DEE+YK  +L  + RS  K+    LIE   F+ +   L+ASLTI+ L    
Sbjct: 148 -----EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLKHHT 207

Query: 224 IWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIW 283
            WGL +WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVLYFV+GL+KSV + IW
Sbjct: 208 FWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIW 267

Query: 284 LALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCA 343
           L L+L+AW LLF+   KRS    ++L  +TR L + L GA  WLVKTLL+KILAA+F   
Sbjct: 268 LCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVN 327

Query: 344 RFFDRIQESIFHQYILRILSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVI 403
            FFDRIQ+S+FHQY+L+ LSG PLME AERVGR  STG LSF  + K+   G   +++VI
Sbjct: 328 NFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKKVI 387

Query: 404 DVDKLKKMKQEKISAWTMRGLINVIRSSGLSTISNTI-ENFKEEENEQKDKEINSEWEAR 463
           D+ K+ KMK+EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE EA 
Sbjct: 388 DMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEAL 447

Query: 464 AAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNV 523
           AAAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G  ETGRI RK    W+V V
Sbjct: 448 AAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKV 507

Query: 524 YVERKSLAHSLNDTKTAIEELNKLASAIVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVV 583
           Y  R++LAHSLNDTKTA+++LNKL +AI+++V ++ WLLL+   TT+VL+FFS+QL+ + 
Sbjct: 508 YTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALA 567

Query: 584 FMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPN 643
           F+ G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++YPN
Sbjct: 568 FIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPN 627

Query: 644 SVLATKPISNYYRSPEMSDAIEFSVDFSTSIESIGALKARIKTYLESKPQFWRPNHSVVV 703
           +VLATKPISNY+RSP M + +EFS+ FST +  I  LK RI  YLE  PQ W P HSVVV
Sbjct: 628 AVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVV 687

Query: 704 KEIENVNKMKLALCVNHTINFQNYGDKSSRRSDLVLELKKIFEDLGIKYHLLPQEVHL 751
           KEIEN+NK+K+AL  +HTI FQ   +++ RR++L L +K++ EDL I Y LLPQ+++L
Sbjct: 688 KEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of HG10007276 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 689.9 bits (1779), Expect = 2.3e-198
Identity = 381/718 (53.06%), Postives = 507/718 (70.61%), Query Frame = 0

Query: 44  RSFRCTIPQSAVG----SSPSHEISRM--TPHKPPKIPGETAI---RRPSFARSSFSKPK 103
           RS     P+S  G     SPS EIS++  +P+KPP+ P +  +   +R SFARS +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 104 SRLIESPCP-DGAYLAEEKAQAKSTFYSSPKVDSPAKITAVTSPKEALKSAPITPKTPLI 163
           SR ++  CP D + L EE  +     +S  +     K     S +     AP+TP   ++
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNNK-----SNRSVGSPAPVTPSKVVV 147

Query: 164 GTTGSEEEDDEEVYKTAELKVKERSGKKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKE 223
                E+++DEE+YK  +L  + RS  K+    LIE   F+ +   L+ASLTI+ L    
Sbjct: 148 -----EKDEDEEIYKKVKLNREMRS--KISTLALIESAFFVVILSALVASLTINVLKHHT 207

Query: 224 IWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIW 283
            WGL +WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVLYFV+GL+KSV + IW
Sbjct: 208 FWGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIW 267

Query: 284 LALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCA 343
           L L+L+AW LLF+   KRS    ++L  +TR L + L GA  WLVKTLL+KILAA+F   
Sbjct: 268 LCLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVN 327

Query: 344 RFFDRIQESIFHQYILRILSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVI 403
            FFDRIQ+S+FHQY+L+ LSG PLME AERVGR  STG LSF  + K+   G   +++VI
Sbjct: 328 NFFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKKVI 387

Query: 404 DVDKLKKMKQEKISAWTMRGLINVIRSSGLSTISNTI-ENFKEEENEQKDKEINSEWEAR 463
           D+ K+ KMK+EK+SAWTMR L+  +R+SGLSTIS+T+ E    E  EQ D+EI SE EA 
Sbjct: 388 DMGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEAL 447

Query: 464 AAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNV 523
           AAAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G  ETGRI RK    W+V V
Sbjct: 448 AAAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKV 507

Query: 524 YVERKSLAHSLNDTKTAIEELNKLASAIVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVV 583
           Y  R++LAHSLNDTKTA+++LNKL +AI+++V ++ WLLL+   TT+VL+FFS+QL+ + 
Sbjct: 508 YTSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALA 567

Query: 584 FMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPN 643
           F+ G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++YPN
Sbjct: 568 FIIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPN 627

Query: 644 SVLATKPISNYYRSPEMSDAIEFSVDFSTSIESIGALKARIKTYLESKPQFWRPNHSVVV 703
           +VLATKPISNY+RSP M + +EFS+ FST +  I  LK RI  YLE  PQ W P HSVVV
Sbjct: 628 AVLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVV 687

Query: 704 KEIENVNKMKLALCVNHTINFQNYGDKSSRRSDLVLELKKIFEDLGIKYHLLPQEVHL 751
           KEIEN+NK+K+AL  +HTI FQ   +++ RR++L L +K++ EDL I Y LLPQ+++L
Sbjct: 688 KEIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of HG10007276 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 615.1 bits (1585), Expect = 7.3e-176
Identity = 350/752 (46.54%), Postives = 494/752 (65.69%), Query Frame = 0

Query: 8   EQLVLRILEGEEGVDASKDLTKPSVASFPDFELKETRSFRCTIPQSAVGSSPSHEISRMT 67
           E++V+ + + E+  D     +   +AS PD +    +S    +P  ++ +   ++ S  +
Sbjct: 10  EEVVINVSDKEDSKDPRASPSFNPLAS-PDSDAGIEKS--KPVPPISIPTPEIYKFSG-S 69

Query: 68  PHKPPKIPG-ETAIRRPSFARSSFSKPKSRLIESPC--------PDGAYLAEEKAQAKST 127
            HKPPKIP  E  +RR S +RS +SKPKSR  E            +G     E+  A S 
Sbjct: 70  VHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQFGAGSF 129

Query: 128 FYSSPKVDSPAKITAVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERS 187
              S    SP             KS        L      E +++EE+YK  +L   +RS
Sbjct: 130 ARGSFDRASPNN-----------KSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRS 189

Query: 188 GKKLKRTVLIEWVAFLCLTGCLIASLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFSQW 247
           G  +K    +E V F+ + G LI SLTID +    IWGL  WKWCVLV+V   G L + W
Sbjct: 190 G--MKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNW 249

Query: 248 FINCLVFLIERNFLLKRKVLYFVYGLRKSVIIVIWLALVLLAWGLLFDQSSKRSKKGNEI 307
           F++ +VF+IE+N+LL++KVLYFV+GL+K+V + IW +LVL+AW  LFD   KR++K    
Sbjct: 250 FMHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKTKRF 309

Query: 308 LNYVTRALGASLIGAGLWLVKTLLVKILAASFQCARFFDRIQESIFHQYILRILSGPPLM 367
           L+++T  + + L+G+ L+LVKT  +K+LA+ F    FF+RIQES+FHQY+L+ LSGPPL+
Sbjct: 310 LDFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLI 369

Query: 368 EMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVI 427
           E AE VGR  STG LSF   K     G    ++VID+ K+ +MKQEK+SAWTMR LI  +
Sbjct: 370 EEAENVGRVPSTGHLSFTRTK----DGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAV 429

Query: 428 RSSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRF 487
            +SG+STIS+T++    ++ E+ DKEI +E EA AAAY +F NVAKP   YI+E+DL RF
Sbjct: 430 GTSGISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIEEDDLLRF 489

Query: 488 MSKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 547
           M KEE+D V+PL E   +TG+I RKT   W+VNVY  RK++ HSLNDTKTA+++L+KL +
Sbjct: 490 MIKEEVDLVLPLIE-DADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLIT 549

Query: 548 AIVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVG 607
            I+ ++  I W++L+   +T++L+ FSSQ L + FM G+T + +FE+ +FVFVMHP+DVG
Sbjct: 550 GILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVG 609

Query: 608 DRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDAIEFSVD 667
           DRCVVDGV ++VEE+++LTT+FL+ DNEK+FYPNSVL +KPISN+YRSP+M D ++F + 
Sbjct: 610 DRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIA 669

Query: 668 FSTSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHTINFQNYGD 727
           FST  E IG LK +I  YL +  Q W P   V+V+ IEN+NK+ L + V HTINFQ Y +
Sbjct: 670 FSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVE 729

Query: 728 KSSRRSDLVLELKKIFEDLGIKYHLLPQEVHL 751
           KS RR+ L++ +K+I EDL I Y LLPQ+V+L
Sbjct: 730 KSLRRTALIIAIKRILEDLEIDYTLLPQDVNL 738

BLAST of HG10007276 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 526.2 bits (1354), Expect = 4.5e-149
Identity = 291/649 (44.84%), Postives = 421/649 (64.87%), Query Frame = 0

Query: 148 PKTPLIGTTGSEE-EDDEEVYKTAELKVKERSGKKLKRTVLIEWVAFLCLTGCLIASLTI 207
           P TP  G  GS++ E++E+ +   +L    R  +K+   V+IEW+  + +   LI SL I
Sbjct: 223 PGTPNQG--GSKDLEEEEDPFSEEDLPEGLRK-EKICVWVIIEWIFLILIIASLICSLVI 282

Query: 208 DKLLTKEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLRK 267
             L  K +W L LWKW V+VLV+ CGRL S W +   V+ +E NFL ++KVLYFVYG+RK
Sbjct: 283 PYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRK 342

Query: 268 SVIIVIWLALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAGLWLVKTLLVKIL 327
            V   +WL LVL+AW  LFD+  +R  + + +L YVT+ L   L+   +WL+KTLLVK+L
Sbjct: 343 PVQNCLWLGLVLIAWHFLFDKKVEREMR-STVLKYVTKVLICLLVAVIIWLIKTLLVKVL 402

Query: 328 AASFQCARFFDRIQESIFHQYILRILSGPPLMEM---AERVGRAASTGQLSFRHLK---- 387
           A+SF  + +FDRIQES+F QY++  LSGPP +E+    E+V     T ++  R L     
Sbjct: 403 ASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGP 462

Query: 388 -----------------------------KESDGGNEGKEEVIDVDKLKKMKQEKISAWT 447
                                          S    EG EE I +D L++M  + +SAW 
Sbjct: 463 KAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWK 522

Query: 448 MRGLINVIRSSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYI 507
           M+ L+NVI+   LST+   I++   +E++ K  +I SE+EA+ AA +IF+NVA+PGS+YI
Sbjct: 523 MKKLMNVIKKGTLSTLDEQIQDTTTQEDD-KATQIRSEFEAKLAARKIFQNVAEPGSRYI 582

Query: 508 DEEDLFRFMSKEEIDNVMPLFEGGVETGRIKRKTLKNWLVNVYVERKSLAHSLNDTKTAI 567
             ED  RF+S++E +  M LFEG  E  +I +  LKNW+VN + ER++LA +LNDTKTA+
Sbjct: 583 YMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAV 642

Query: 568 EELNKLASAIVLIVIIIEWLLLMGFLTTQVLVFFSSQLLLVVFMFGNTARTVFEAIIFVF 627
             L+++   +V IVI+I WLL++G  TT+ L+  SSQLLLVVF+FGN+ +T+FEA+IFVF
Sbjct: 643 NRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVF 702

Query: 628 VMHPFDVGDRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMS 687
           VMHPFDVGDRC +DGVQM+VEEMNILTT+FLR+DN+KI YPNS+L TKPI+NYYRSP+M 
Sbjct: 703 VMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQ 762

Query: 688 DAIEFSVDFSTSIESIGALKARIKTYLESKPQFWRPNHSVVVKEIENVNKMKLALCVNHT 747
           DAIEF V  +T  E   AL+ RI +Y+++K   W P+  +V +++  +N +K+A+   H 
Sbjct: 763 DAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHK 822

Query: 748 INFQNYGDKSSRRSDLVLELKKIFEDLGIKYHLLPQEVHLNYVGSAAPV 760
           +N QN G++  RR  L+ E+ ++  +L I+Y L P  +++  + +A P+
Sbjct: 823 MNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATPI 866

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878911.10.0e+0095.82mechanosensitive ion channel protein 10-like [Benincasa hispida][more]
XP_031738755.10.0e+0092.75mechanosensitive ion channel protein 10 [Cucumis sativus] >KGN66279.1 hypothetic... [more]
XP_008450905.10.0e+0092.65PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo] >XP_00845... [more]
KAA0055793.10.0e+0092.52mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa][more]
XP_022987285.10.0e+0089.08mechanosensitive ion channel protein 10-like [Cucurbita maxima] >XP_022987286.1 ... [more]
Match NameE-valueIdentityDescription
Q9LYG93.3e-19753.06Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10... [more]
Q84M971.0e-17446.54Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 P... [more]
Q9LPG36.3e-14844.84Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 P... [more]
Q9LH747.0e-14743.27Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 P... [more]
Q9SYM15.5e-14442.98Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 P... [more]
Match NameE-valueIdentityDescription
A0A0A0LYZ90.0e+0092.75Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_1G595830 ... [more]
A0A1S3BR080.0e+0092.65Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103492355 PE=... [more]
A0A5D3CFQ70.0e+0092.65Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5A7UIR30.0e+0092.52Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1JDR10.0e+0089.08Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111484882... [more]
Match NameE-valueIdentityDescription
AT5G12080.12.3e-19853.06mechanosensitive channel of small conductance-like 10 [more]
AT5G12080.22.3e-19853.06mechanosensitive channel of small conductance-like 10 [more]
AT5G12080.32.3e-19853.06mechanosensitive channel of small conductance-like 10 [more]
AT5G19520.17.3e-17646.54mechanosensitive channel of small conductance-like 9 [more]
AT1G53470.14.5e-14944.84mechanosensitive channel of small conductance-like 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 423..443
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 142..161
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 51..98
NoneNo IPR availablePANTHERPTHR31618:SF7MECHANOSENSITIVE ION CHANNEL PROTEINcoord: 4..756
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 594..643
e-value: 5.9E-12
score: 47.3
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 535..740
e-value: 2.1E-23
score: 82.9
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPIRSFPIRSF017209Memb_At2g17000coord: 17..764
e-value: 5.5E-217
score: 721.0
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPANTHERPTHR31618MECHANOSENSITIVE ION CHANNEL PROTEIN 5coord: 4..756
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 584..643

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10007276.1HG10007276.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006811 ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane