HG10006949 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10006949 vs. NCBI nr
Match: XP_038876538.1 (mitotic spindle checkpoint protein MAD1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1267.3 bits (3278), Expect = 0.0e+00
Identity = 690/727 (94.91%), Postives = 707/727 (97.25%), Query Frame = 0

Query: 1   MIVRTPPPKKQRSDVTSLPDSSSAGASDLPLVIYEDPLPLVPATTQPGLSQEPSEHMLCT 60
           MIVRTPPPKKQRSDV SLPDS  AGASDLPLVIYEDPLPLVPA TQPG SQE SEHMLCT
Sbjct: 1   MIVRTPPPKKQRSDVASLPDSLPAGASDLPLVIYEDPLPLVPAMTQPGCSQESSEHMLCT 60

Query: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGVLTENLSKIESERKKFLDQLLYTEQELAAA 120
           YQCRQMVKSDFLDALSNAEKQVHDYELKLGVL ENLSKIESERKKFLDQLLYTEQELAAA
Sbjct: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGVLNENLSKIESERKKFLDQLLYTEQELAAA 120

Query: 121 RGREKVLQEQLIKEINDSGERLKKQMQISSELEVKLQNESNLRMQAESSIALSEEKARLL 180
           RGREKVLQEQLIKE+NDSGERLKKQMQISSELEVKLQNESNLRM+AESSIALSEEKARLL
Sbjct: 121 RGREKVLQEQLIKEVNDSGERLKKQMQISSELEVKLQNESNLRMKAESSIALSEEKARLL 180

Query: 181 EDKLNHLSESIERERKRLHSELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKVQ 240
           EDKLNHLSESIERE+KRLH+ELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLK Q
Sbjct: 181 EDKLNHLSESIEREKKRLHTELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKEQ 240

Query: 241 LEDLKKQLNECLSQKTELEKKLASFTVNQGTGKESNILIKHLQEELRNYESEVREARKLK 300
           LEDLKKQLNECL QKTELEKKL SFTVN+GTGKESNILIKHLQEELRNYESEVREARKLK
Sbjct: 241 LEDLKKQLNECLCQKTELEKKLTSFTVNEGTGKESNILIKHLQEELRNYESEVREARKLK 300

Query: 301 SSLGDIGLLKEKLLEEKARRERVESELSNLQEIQLSVKNLEDELTRRELLIKDIPGITTY 360
           SSLGDIGLLKEKLLEEKARRER ESELS L++IQLSVKNLEDELTRRELLIKDIPGITTY
Sbjct: 301 SSLGDIGLLKEKLLEEKARRERAESELSKLEDIQLSVKNLEDELTRRELLIKDIPGITTY 360

Query: 361 EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVVLDAAQIDKQKAESEATLVKEKIEALK 420
           EDIP KISSLQKEV+DN IKMGEVNARLKQLEV LDAAQIDK+KAESEATLVKEKI+ALK
Sbjct: 361 EDIPIKISSLQKEVLDNMIKMGEVNARLKQLEVALDAAQIDKRKAESEATLVKEKIDALK 420

Query: 421 LEVKQNELLLSVATEERDKLKSLVNELRSLKNDDAEAKATNETLSQELDSTLAKKDWYIK 480
           LEVKQNELLLSVATEERDKL+ LVNEL+SLKNDDAEAKAT+ETLSQELDSTLAKKDWYIK
Sbjct: 421 LEVKQNELLLSVATEERDKLRCLVNELKSLKNDDAEAKATSETLSQELDSTLAKKDWYIK 480

Query: 481 ELESNLHEQKEVSSRQHDELKLLNERLNGEAKRIKSLERDCDRLRSEISLLESKLGHGDF 540
           ELE+NLHEQKEV+SRQHDELKLLNER N EAK+IKSLERDCDRLRSEISLLESKLGHGDF
Sbjct: 481 ELETNLHEQKEVNSRQHDELKLLNERSNNEAKKIKSLERDCDRLRSEISLLESKLGHGDF 540

Query: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600
           SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS
Sbjct: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600

Query: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660
           GKI+QLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRF 
Sbjct: 601 GKILQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFI 660

Query: 661 LLSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720
           L SIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES
Sbjct: 661 LQSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720

Query: 721 FNRRTLS 728
           FNRRTLS
Sbjct: 721 FNRRTLS 727

BLAST of HG10006949 vs. NCBI nr
Match: KAG6601670.1 (Mitotic spindle checkpoint protein MAD1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032439.1 Mitotic spindle checkpoint protein MAD1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1256.5 bits (3250), Expect = 0.0e+00
Identity = 685/727 (94.22%), Postives = 702/727 (96.56%), Query Frame = 0

Query: 1   MIVRTPPPKKQRSDVTSLPDSSSAGASDLPLVIYEDPLPLVPATTQPGLSQEPSEHMLCT 60
           MIVRTPPPKKQRSDV +LPDSSSAGASDLPLVIYEDPLP+VPATTQP  S EPSE MLCT
Sbjct: 1   MIVRTPPPKKQRSDVRALPDSSSAGASDLPLVIYEDPLPVVPATTQPECSHEPSEQMLCT 60

Query: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGVLTENLSKIESERKKFLDQLLYTEQELAAA 120
           YQCRQMVK+DFLDALSNAEKQVHDYELKLGVL ENLSK+ESERKKFLDQLLYTEQELAAA
Sbjct: 61  YQCRQMVKADFLDALSNAEKQVHDYELKLGVLNENLSKVESERKKFLDQLLYTEQELAAA 120

Query: 121 RGREKVLQEQLIKEINDSGERLKKQMQISSELEVKLQNESNLRMQAESSIALSEEKARLL 180
           RGREKVLQEQLIKEINDSGE+LKKQMQISSELEVKLQNESNLRM+AESSIALSEEKA+LL
Sbjct: 121 RGREKVLQEQLIKEINDSGEQLKKQMQISSELEVKLQNESNLRMKAESSIALSEEKAKLL 180

Query: 181 EDKLNHLSESIERERKRLHSELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKVQ 240
           EDKLNH SESIERERKRLH ELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLK Q
Sbjct: 181 EDKLNHFSESIERERKRLHGELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKEQ 240

Query: 241 LEDLKKQLNECLSQKTELEKKLASFTVNQGTGKESNILIKHLQEELRNYESEVREARKLK 300
           LEDLKKQLNECL QK+ELEKKLASFTVN+GT KESNILIKHLQEELRNYESEVREARKLK
Sbjct: 241 LEDLKKQLNECLCQKSELEKKLASFTVNEGTSKESNILIKHLQEELRNYESEVREARKLK 300

Query: 301 SSLGDIGLLKEKLLEEKARRERVESELSNLQEIQLSVKNLEDELTRRELLIKDIPGITTY 360
           SSLGDIGLLKEKLLEEKARRER ESELS LQ+IQLSVKNLEDELTRRELLIKDIPGITTY
Sbjct: 301 SSLGDIGLLKEKLLEEKARRERAESELSKLQDIQLSVKNLEDELTRRELLIKDIPGITTY 360

Query: 361 EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVVLDAAQIDKQKAESEATLVKEKIEALK 420
           EDIP KISSLQKEVIDNTIKMGEVNARLKQLEV LDAAQ DKQKAESEATL KEKIEALK
Sbjct: 361 EDIPNKISSLQKEVIDNTIKMGEVNARLKQLEVALDAAQTDKQKAESEATLAKEKIEALK 420

Query: 421 LEVKQNELLLSVATEERDKLKSLVNELRSLKNDDAEAKATNETLSQELDSTLAKKDWYIK 480
           LE +QNELLLSVATEERDKLKSLVNEL+SLKN DAEAKAT+ET+SQELDS LAKKDWYIK
Sbjct: 421 LETQQNELLLSVATEERDKLKSLVNELKSLKNGDAEAKATSETISQELDSALAKKDWYIK 480

Query: 481 ELESNLHEQKEVSSRQHDELKLLNERLNGEAKRIKSLERDCDRLRSEISLLESKLGHGDF 540
           ELESNLHEQK+V+ RQHDEL+LLNERLN EAKRIKSLERD DRLRSEISLLESKLGHGDF
Sbjct: 481 ELESNLHEQKQVNGRQHDELRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKLGHGDF 540

Query: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600
           SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS
Sbjct: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600

Query: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660
           GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR DGIPVTRFT
Sbjct: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRTDGIPVTRFT 660

Query: 661 LLSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720
           L SIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES
Sbjct: 661 LQSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720

Query: 721 FNRRTLS 728
           FNRRTLS
Sbjct: 721 FNRRTLS 727

BLAST of HG10006949 vs. NCBI nr
Match: XP_008460594.1 (PREDICTED: mitotic spindle checkpoint protein MAD1 [Cucumis melo])

HSP 1 Score: 1255.0 bits (3246), Expect = 0.0e+00
Identity = 683/727 (93.95%), Postives = 705/727 (96.97%), Query Frame = 0

Query: 1   MIVRTPPPKKQRSDVTSLPDSSSAGASDLPLVIYEDPLPLVPATTQPGLSQEPSEHMLCT 60
           MIVRTPPPKKQRSDVTSLPDSS A ASDLPLVIYEDPLPLVPATTQP  S  PS+HMLCT
Sbjct: 1   MIVRTPPPKKQRSDVTSLPDSSPAAASDLPLVIYEDPLPLVPATTQPASSHLPSDHMLCT 60

Query: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGVLTENLSKIESERKKFLDQLLYTEQELAAA 120
           YQCRQMVKSDFLDALSNAEKQVHDYELKLG+L ENLSK+ESERKKFLDQLLYTEQELAAA
Sbjct: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGMLNENLSKVESERKKFLDQLLYTEQELAAA 120

Query: 121 RGREKVLQEQLIKEINDSGERLKKQMQISSELEVKLQNESNLRMQAESSIALSEEKARLL 180
           RGREKVLQEQLIKEI+DSGERLKKQ+Q SSELEVKLQNESNLR++AESSIA SEEKARLL
Sbjct: 121 RGREKVLQEQLIKEISDSGERLKKQLQTSSELEVKLQNESNLRIKAESSIASSEEKARLL 180

Query: 181 EDKLNHLSESIERERKRLHSELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKVQ 240
           EDKLNHLSESIERERK L +ELAQL+GESKLSVSRINADL KMVCRASNAEKESELLK Q
Sbjct: 181 EDKLNHLSESIERERKHLDTELAQLRGESKLSVSRINADLAKMVCRASNAEKESELLKEQ 240

Query: 241 LEDLKKQLNECLSQKTELEKKLASFTVNQGTGKESNILIKHLQEELRNYESEVREARKLK 300
           LEDLKKQLNECL QK+ELEKKLASFTVN+GTGKESNILIKHLQEELRNYESEV+EARKLK
Sbjct: 241 LEDLKKQLNECLCQKSELEKKLASFTVNEGTGKESNILIKHLQEELRNYESEVKEARKLK 300

Query: 301 SSLGDIGLLKEKLLEEKARRERVESELSNLQEIQLSVKNLEDELTRRELLIKDIPGITTY 360
           SSLGDIGLLKEKLLEEKARRER +SELS LQ+IQLSVKNLEDELTRR+LLIKDIPGI+TY
Sbjct: 301 SSLGDIGLLKEKLLEEKARRERADSELSKLQDIQLSVKNLEDELTRRDLLIKDIPGISTY 360

Query: 361 EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVVLDAAQIDKQKAESEATLVKEKIEALK 420
           EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEV LDAAQIDKQKAESEATLVKEKIEALK
Sbjct: 361 EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVALDAAQIDKQKAESEATLVKEKIEALK 420

Query: 421 LEVKQNELLLSVATEERDKLKSLVNELRSLKNDDAEAKATNETLSQELDSTLAKKDWYIK 480
           LEV+QNELLLSVATEERDKLKSLVNEL++LKNDDAEAKATNETLSQELDS  AKKDWYIK
Sbjct: 421 LEVQQNELLLSVATEERDKLKSLVNELKTLKNDDAEAKATNETLSQELDSIFAKKDWYIK 480

Query: 481 ELESNLHEQKEVSSRQHDELKLLNERLNGEAKRIKSLERDCDRLRSEISLLESKLGHGDF 540
           ELESNLHEQKEV+SRQHDELKLLNERLN +AKRIKSLERDCDRLRSEISLLESK+GHGDF
Sbjct: 481 ELESNLHEQKEVNSRQHDELKLLNERLNNDAKRIKSLERDCDRLRSEISLLESKIGHGDF 540

Query: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600
           SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELK PSGDAGKLVDSYIS
Sbjct: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKAPSGDAGKLVDSYIS 600

Query: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660
           GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT
Sbjct: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660

Query: 661 LLSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720
           L SIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES
Sbjct: 661 LQSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720

Query: 721 FNRRTLS 728
           FNRRTLS
Sbjct: 721 FNRRTLS 727

BLAST of HG10006949 vs. NCBI nr
Match: XP_004142360.1 (mitotic spindle checkpoint protein MAD1 isoform X1 [Cucumis sativus] >KAE8648888.1 hypothetical protein Csa_008623 [Cucumis sativus])

HSP 1 Score: 1249.6 bits (3232), Expect = 0.0e+00
Identity = 681/727 (93.67%), Postives = 702/727 (96.56%), Query Frame = 0

Query: 1   MIVRTPPPKKQRSDVTSLPDSSSAGASDLPLVIYEDPLPLVPATTQPGLSQEPSEHMLCT 60
           MIVRTPPPKKQRSDVTSLPDSS A ASDLPLVIYEDPLPLVPATT+P  S EPS+HMLCT
Sbjct: 1   MIVRTPPPKKQRSDVTSLPDSSPAAASDLPLVIYEDPLPLVPATTEPASSHEPSDHMLCT 60

Query: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGVLTENLSKIESERKKFLDQLLYTEQELAAA 120
           YQCRQMVKSDFLDALSNAEKQVHDYELKLGVL ENLSK+ESERKKFLDQLLYTEQELAAA
Sbjct: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGVLNENLSKVESERKKFLDQLLYTEQELAAA 120

Query: 121 RGREKVLQEQLIKEINDSGERLKKQMQISSELEVKLQNESNLRMQAESSIALSEEKARLL 180
           R REKVLQEQLIKEI+DSGERLKKQMQISSELEV+LQNESNLR++AESSIA SEEKARLL
Sbjct: 121 RRREKVLQEQLIKEISDSGERLKKQMQISSELEVRLQNESNLRIKAESSIASSEEKARLL 180

Query: 181 EDKLNHLSESIERERKRLHSELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKVQ 240
           EDKLNHLSESIERERK L +ELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLK Q
Sbjct: 181 EDKLNHLSESIERERKHLDTELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKGQ 240

Query: 241 LEDLKKQLNECLSQKTELEKKLASFTVNQGTGKESNILIKHLQEELRNYESEVREARKLK 300
           LEDLK QLNECL QK+ELEKKLASFTVN+GTGKESNILIKHLQEELRNYESEV+EARKLK
Sbjct: 241 LEDLKNQLNECLCQKSELEKKLASFTVNEGTGKESNILIKHLQEELRNYESEVKEARKLK 300

Query: 301 SSLGDIGLLKEKLLEEKARRERVESELSNLQEIQLSVKNLEDELTRRELLIKDIPGITTY 360
           SSLGDIGLLKEKLLEEKARRER +SELS LQ+IQLSVKNLEDELTRR+LLI  IPGI+TY
Sbjct: 301 SSLGDIGLLKEKLLEEKARRERADSELSKLQDIQLSVKNLEDELTRRDLLINSIPGISTY 360

Query: 361 EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVVLDAAQIDKQKAESEATLVKEKIEALK 420
           EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEV LDAAQIDKQKAESEATLV+EKIEALK
Sbjct: 361 EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVALDAAQIDKQKAESEATLVEEKIEALK 420

Query: 421 LEVKQNELLLSVATEERDKLKSLVNELRSLKNDDAEAKATNETLSQELDSTLAKKDWYIK 480
           LEVKQNELLLSVATEERDKLKSLVNEL++LKNDDAEAK T ETLSQELD TLAKKDWYIK
Sbjct: 421 LEVKQNELLLSVATEERDKLKSLVNELKTLKNDDAEAKETKETLSQELDLTLAKKDWYIK 480

Query: 481 ELESNLHEQKEVSSRQHDELKLLNERLNGEAKRIKSLERDCDRLRSEISLLESKLGHGDF 540
           ELESNLHEQKEV+SRQHDELKLLNERLN +AKRIKSLERDCDRLRSEISLLESK+GHGDF
Sbjct: 481 ELESNLHEQKEVNSRQHDELKLLNERLNNDAKRIKSLERDCDRLRSEISLLESKIGHGDF 540

Query: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600
           SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELK PSGDAGKLVDSYIS
Sbjct: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKAPSGDAGKLVDSYIS 600

Query: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660
           GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT
Sbjct: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660

Query: 661 LLSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720
           L SIYAQSDDEKLQFEYESGNTNIL NNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES
Sbjct: 661 LQSIYAQSDDEKLQFEYESGNTNILVNNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720

Query: 721 FNRRTLS 728
           FNRRTLS
Sbjct: 721 FNRRTLS 727

BLAST of HG10006949 vs. NCBI nr
Match: XP_022933081.1 (mitotic spindle checkpoint protein MAD1 [Cucurbita moschata])

HSP 1 Score: 1248.0 bits (3228), Expect = 0.0e+00
Identity = 678/727 (93.26%), Postives = 702/727 (96.56%), Query Frame = 0

Query: 1   MIVRTPPPKKQRSDVTSLPDSSSAGASDLPLVIYEDPLPLVPATTQPGLSQEPSEHMLCT 60
           MIVRTPPPKKQRSDV +LPDSSSAGASDLPLVIYEDPLP+VPATTQP  S EPSE MLCT
Sbjct: 1   MIVRTPPPKKQRSDVRALPDSSSAGASDLPLVIYEDPLPVVPATTQPECSHEPSEQMLCT 60

Query: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGVLTENLSKIESERKKFLDQLLYTEQELAAA 120
           YQCRQMVK+DFLDALSNAEKQVHDYELKLGVL ENLSK+ESERKKFL+QLLYTEQELAAA
Sbjct: 61  YQCRQMVKADFLDALSNAEKQVHDYELKLGVLNENLSKVESERKKFLEQLLYTEQELAAA 120

Query: 121 RGREKVLQEQLIKEINDSGERLKKQMQISSELEVKLQNESNLRMQAESSIALSEEKARLL 180
           RGREKVLQEQLIKEINDSGE+LKKQMQISSELEVKLQNE+NLRM+AESSIALSEEKA+LL
Sbjct: 121 RGREKVLQEQLIKEINDSGEQLKKQMQISSELEVKLQNEANLRMKAESSIALSEEKAKLL 180

Query: 181 EDKLNHLSESIERERKRLHSELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKVQ 240
           EDKLNH SESIERERKRLH ELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLK Q
Sbjct: 181 EDKLNHFSESIERERKRLHGELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKEQ 240

Query: 241 LEDLKKQLNECLSQKTELEKKLASFTVNQGTGKESNILIKHLQEELRNYESEVREARKLK 300
           LEDLKKQLNECL QK+ELEKKLASFTVN+GT KESNILIKHLQEELRNYESEVREARKLK
Sbjct: 241 LEDLKKQLNECLCQKSELEKKLASFTVNEGTSKESNILIKHLQEELRNYESEVREARKLK 300

Query: 301 SSLGDIGLLKEKLLEEKARRERVESELSNLQEIQLSVKNLEDELTRRELLIKDIPGITTY 360
           SSLGDIGLLKEKLLEEKARRER ESEL+ LQ+IQLSVKNLEDELTRRELLIKDIPGITTY
Sbjct: 301 SSLGDIGLLKEKLLEEKARRERAESELTKLQDIQLSVKNLEDELTRRELLIKDIPGITTY 360

Query: 361 EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVVLDAAQIDKQKAESEATLVKEKIEALK 420
           EDIP KISSLQKEVIDNTIKMGEVNARLKQLEV LDAAQ DKQKAESEATL +EKIEAL+
Sbjct: 361 EDIPNKISSLQKEVIDNTIKMGEVNARLKQLEVALDAAQTDKQKAESEATLAREKIEALR 420

Query: 421 LEVKQNELLLSVATEERDKLKSLVNELRSLKNDDAEAKATNETLSQELDSTLAKKDWYIK 480
           LE +QNELLLS+ATEERDKLK+LVNEL+SLKN DAEAKAT+ET+SQELDS LAKKDWYIK
Sbjct: 421 LETQQNELLLSLATEERDKLKNLVNELKSLKNGDAEAKATSETISQELDSALAKKDWYIK 480

Query: 481 ELESNLHEQKEVSSRQHDELKLLNERLNGEAKRIKSLERDCDRLRSEISLLESKLGHGDF 540
           ELESNLHEQK+V+ RQHDEL+LLNERLN EAKRIKSLERD DRLRSEISLLESKLGHGDF
Sbjct: 481 ELESNLHEQKQVNGRQHDELRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKLGHGDF 540

Query: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600
           SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS
Sbjct: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600

Query: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660
           GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR DGIPVTRFT
Sbjct: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRTDGIPVTRFT 660

Query: 661 LLSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720
           L SIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES
Sbjct: 661 LQSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720

Query: 721 FNRRTLS 728
           FNRRTLS
Sbjct: 721 FNRRTLS 727

BLAST of HG10006949 vs. ExPASy Swiss-Prot
Match: Q9LTY1 (Mitotic spindle checkpoint protein MAD1 OS=Arabidopsis thaliana OX=3702 GN=MAD1 PE=1 SV=1)

HSP 1 Score: 818.5 bits (2113), Expect = 5.9e-236
Identity = 459/727 (63.14%), Postives = 578/727 (79.50%), Query Frame = 0

Query: 1   MIVRTPPPKKQRSDVTSLPDSSSAGASDLPLVIYED-PLPLVPATTQPGLSQEPSEHMLC 60
           MI+RTP PK+ RSD    P  + A  S   L+IYED PLP  PA  Q        +H LC
Sbjct: 1   MILRTPQPKRLRSDAGESPFPTGATGSGNQLIIYEDSPLP-APAPLQTSHDHSADQH-LC 60

Query: 61  TYQCRQMVKSDFLDALSNAEKQVHDYELKLGVLTENLSKIESERKKFLDQLLYTEQELAA 120
           TYQCRQMVK+D LDALS AEKQV + + KL  L  N ++ ++ERK F D+ LY+EQELAA
Sbjct: 61  TYQCRQMVKADVLDALSTAEKQVEESKTKLQTLNANFTEADAERKHFRDKFLYSEQELAA 120

Query: 121 ARGREKVLQEQLIKEINDSGERLKKQMQISSELEVKLQNESNLRMQAESSIALSEEKARL 180
           A+ REK+LQEQL+ EIN+S ER  K++Q   ELEVKLQNE NLR +AESS A +EEKA+L
Sbjct: 121 AKAREKMLQEQLLMEINNSQERYTKELQSCHELEVKLQNEMNLRKKAESSAATAEEKAKL 180

Query: 181 LEDKLNHLSESIERERKRLHSELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKV 240
           LEDKL  LS S++RE+KRL++++AQL  E+KLSV+RI ADLE+M CRA NAE ES LL+ 
Sbjct: 181 LEDKLTQLSGSVDREKKRLNNDIAQLGKEAKLSVARIGADLERMQCRAQNAETESNLLRS 240

Query: 241 QLEDLKKQLNECLSQKTELEKKLASFTVNQGTGKESNILIKHLQEELRNYESEVREARKL 300
           QLE LK   +ECL +KTE++KKL+SFT    +  ++++L+KHLQEEL+ YE+EVREARKL
Sbjct: 241 QLEHLKLIFDECLQEKTEVDKKLSSFTSEAASSSDNSVLVKHLQEELKRYEAEVREARKL 300

Query: 301 KSSLGDIGLLKEKLLEEKARRERVESELSNLQEIQLSVKNLEDELTRRELLIKDIPGITT 360
           KS   D  LL   LLEE++RRER ESELS   ++QLS++ LE+EL+  + L+ DIPG++ 
Sbjct: 301 KSRHLDAELLNVNLLEEQSRRERAESELSKFHDLQLSMEKLENELSSWKSLLNDIPGVSC 360

Query: 361 YEDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVVLDAAQIDKQKAESEATLVKEKIEAL 420
            +DI  + S LQ EV+ +T+K+GE + R+KQLE  L+A Q+ +Q A SEA L KEK EAL
Sbjct: 361 PDDIVMRFSVLQNEVVQSTMKIGEASTRIKQLEETLEAIQLGRQNAVSEAALAKEKSEAL 420

Query: 421 KLEVKQNELLLSVATEERDKLKSLVNELRSLKNDDAEAKATNETLSQELDSTLAKKDWYI 480
           K +VK+ E++L++ TEE+++LK++VNELR   ++ + + A +  L Q  +S+LAKK+ YI
Sbjct: 421 KTDVKRIEVMLTLVTEEKEQLKAVVNELRKSNSEGSVSGAADGALIQGFESSLAKKENYI 480

Query: 481 KELESNLHEQKEVSSRQHDELKLLNERLNGEAKRIKSLERDCDRLRSEISLLESKLGHGD 540
           K+LE +L++ K+V++RQ  E++LLNE+L  EA+R KSLERD DRLRSEISLLESKLGHGD
Sbjct: 481 KDLEQDLNQLKDVNNRQRTEIELLNEKLVDEARRNKSLERDSDRLRSEISLLESKLGHGD 540

Query: 541 FSSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYI 600
           +S+ NT+VLRMVNTL V+NEAKQTIEAL++ELQKTKE+LQAVEELK+ SGDAGKLVDS+I
Sbjct: 541 YSAANTRVLRMVNTLGVENEAKQTIEALQAELQKTKERLQAVEELKSQSGDAGKLVDSHI 600

Query: 601 SGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRF 660
           +GKI QLKEQ ATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR +GIPVTRF
Sbjct: 601 TGKIAQLKEQNATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRPNGIPVTRF 660

Query: 661 TLLSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVE 720
           TL SIYAQSDDEKL+FEYESGNT+IL N Y SQ ++++Q+EIFIRK NSIPAFTANLT+E
Sbjct: 661 TLQSIYAQSDDEKLEFEYESGNTSILNNEYASQGDIAKQIEIFIRKFNSIPAFTANLTME 720

Query: 721 SFNRRTL 727
           SFNRRTL
Sbjct: 721 SFNRRTL 725

BLAST of HG10006949 vs. ExPASy Swiss-Prot
Match: Q9Y6D9 (Mitotic spindle assembly checkpoint protein MAD1 OS=Homo sapiens OX=9606 GN=MAD1L1 PE=1 SV=2)

HSP 1 Score: 93.6 bits (231), Expect = 9.9e-18
Identity = 171/723 (23.65%), Postives = 329/723 (45.50%), Query Frame = 0

Query: 43  ATTQPGLSQEPSEHMLCTYQCRQMVKSDFLDALSNAEKQVHDYELKLGVLTENLSKIESE 102
           +T+ PG  Q   +  +   +  + ++S     L   E++    EL        L +  S 
Sbjct: 34  STSAPGSLQMQYQQSMQLEERAEQIRSK--SHLIQVEREKMQMELSHKRARVELERAAST 93

Query: 103 RKKFLDQLLYTEQELAAARGREKVLQEQLIKEINDSGERLKKQMQISSELEVKLQNESNL 162
             +  ++ +   QEL     R + LQE+       + E++++Q++ + + +  L   S  
Sbjct: 94  SARNYEREVDRNQELLT---RIRQLQEREA----GAEEKMQEQLERNRQCQQNLDAASKR 153

Query: 163 RMQAESSIALSEEKARLLEDKLNHLSESI---ERERKRLHSELAQLKGESKLSVSRINAD 222
             + E S+A + E    L+ +++ L  S+   E   KRL SE  +L+ +          D
Sbjct: 154 LREKEDSLAQAGETINALKGRISELQWSVMDQEMRVKRLESEKQELQEQ---------LD 213

Query: 223 LEKMVCRASNAE-KESELLKVQLEDLKKQLNECLSQKTELEKKLASFTVNQGTGKESNIL 282
           L+   C+ +N + +E +  +    D ++Q+ + L QK  L+++ A+   N    K   + 
Sbjct: 214 LQHKKCQEANQKIQELQASQEARADHEQQIKD-LEQKLSLQEQDAAIVKNM---KSELVR 273

Query: 283 IKHLQEELRNYESEVREARKLKSSLGDIGLLKEKLLEEKARRERVESELSNLQEIQLSVK 342
           +  L+ EL+    E    R+++ +    GLL+E+L   + +  R E     L  ++L  +
Sbjct: 274 LPRLERELKQLREESAHLREMRET---NGLLQEELEGLQRKLGRQEKMQETLVGLELENE 333

Query: 343 NLEDELTRRELLIKDIP-GITTYEDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVVLDA 402
            L  +L   E L + +   I T ED+   +  LQ+  +    K   V +  + LE     
Sbjct: 334 RLLAKLQSWERLDQTMGLSIRTPEDLSRFVVELQQRELALKDKNSAVTSSARGLEKARQQ 393

Query: 403 AQIDKQKAES---EATLVKEKIEALKLEVKQNELLLSVATEERDKLKSLVNELRS-LKND 462
            Q + ++      E    +E  EAL   +++  LLL   T+ERD +++++    S L   
Sbjct: 394 LQEELRQVSGQLLEERKKRETHEALARRLQKRVLLL---TKERDGMRAILGSYDSELTPA 453

Query: 463 DAEAKATN-----ETLSQELDSTLAKKDWYIKELESNLHEQKEVSSRQHDELKLLNERLN 522
           +   + T      E + Q++ S  A+ +  + +    L  QK+ +     ELK+L  + +
Sbjct: 454 EYSPQLTRRMREAEDMVQKVHSHSAEMEAQLSQALEELGGQKQRADMLEMELKMLKSQSS 513

Query: 523 GEAK--------------RIKSLERDCDRLRSEISLLESKLG----HGDFSSTNTKVLRM 582
              +              +++ LE +  RL  E  +LE++L      GD+  + TKVL M
Sbjct: 514 SAEQSFLFSREEADTLRLKVEELEGERSRLEEEKRMLEAQLERRALQGDYDQSRTKVLHM 573

Query: 583 -VNTLAVDNE-AKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYISGK-IMQLK 642
            +N  +V  +  ++    L++E ++ +  L+A+E   T   D      S  S K + +LK
Sbjct: 574 SLNPTSVARQRLREDHSQLQAECERLRGLLRAMERGGTVPADLEAAAASLPSSKEVAELK 633

Query: 643 EQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFTLLSIYAQ 702
           +Q+ + E + +R K VF  +I  FR+AC  L GY+I        D     ++ L S+YA+
Sbjct: 634 KQVESAELKNQRLKEVFQTKIQEFRKACYTLTGYQI--------DITTENQYRLTSLYAE 693

Query: 703 SDDEKLQFEYES---GNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVESFNRR 728
              + L F+  S       +L   ++    +   +E+ +R+ +SIPAF ++LT+E F+R+
Sbjct: 694 HPGDCLIFKATSPSGSKMQLLETEFSH--TVGELIEVHLRRQDSIPAFLSSLTLELFSRQ 718

BLAST of HG10006949 vs. ExPASy Swiss-Prot
Match: Q80YF0 (Mitotic spindle assembly checkpoint protein MAD1 OS=Cricetulus griseus OX=10029 GN=MAD1L1 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.9e-14
Identity = 153/686 (22.30%), Postives = 307/686 (44.75%), Query Frame = 0

Query: 92  LTENLSKIESERKKFLDQLLYTEQELAAARGREKVLQEQLIKEINDSGERLKKQMQISSE 151
           L E   +I S  K +L Q+   + ++  +  R +V   +L +  N S    ++++  + E
Sbjct: 51  LEERAEQIRS--KSYLIQMEREKMQMELSHKRARV---ELERAANTSARNYEREVDRNQE 110

Query: 152 LEVKLQNESNLRMQAESSIALSEEKARLLEDKLNHLSESIERERK----RLHSELAQLKG 211
           L  +++        AE  +    E+ RL +  L+  S+ + RER+         ++ LKG
Sbjct: 111 LLARIRQLQEREAAAEEKMQEQLERHRLCKQSLDAASQQL-REREDGLAAARETISSLKG 170

Query: 212 ESKLSVSRINADLEKMVCRASNAEKESELLKVQLEDLKKQLNECLSQKTEL--------- 271
             ++S  ++NA  +K+  +    E E + LK QLE  +++  E   +  EL         
Sbjct: 171 --RVSEMQLNAMDQKV--QVKRLESEKQELKEQLELQQRKCQEASQKIQELQASQEERAD 230

Query: 272 -EKKLASFTVNQGTGKESNILIKHLQEELRNYESEVREARKLKSSLGDI-------GLLK 331
            E+K+          ++   ++K+++ EL       RE ++L+     +       GLL 
Sbjct: 231 HEQKIKDLEQKLCLQEQDAAVVKNMKSELLRLPRMERELKRLREENTHLREMKETNGLLT 290

Query: 332 EKLLEEKARRERVESELSNLQEIQLSVKNLEDELTRRELLIKDI-PGITTYEDIPTKISS 391
           E+L   + +  R E     L +++L  + L  +L   E L + +   + T ED+   +  
Sbjct: 291 EELEGLQRKLGRQEKMQEALVDLELEKEKLLAKLQSWEKLDQTMGVNLRTPEDLSRFVVE 350

Query: 392 LQKEVIDNTIKMGEVNARLKQLEVVLDAAQIDKQKAESEATLVKEKIEALKLEVKQNELL 451
           LQ+  +    K   + +  + LE      Q + ++  ++    ++K E  +   ++ +  
Sbjct: 351 LQQRELTLKEKNNTITSSARGLEKAQQQLQDEVRQVSAQLLEERKKREIHEALARRLQKR 410

Query: 452 LSVATEERDKLKSLVNELRS-LKNDDAEAKATN-----ETLSQELDSTLAKKDWYIKELE 511
           + + T+ERD +++++    S L   +  A+ T      E + Q++ +  ++ +  + +  
Sbjct: 411 IVLLTKERDGMRAILGSYDSELTQAEYSAQLTQRMWEAEDMVQKVHAHSSEMETQLSQAL 470

Query: 512 SNLHEQKEVSSRQHDELKLL--------------NERLNGEAKRIKSLERDCDRLRSEIS 571
             L  QK+ +     ELK+L               E ++    +++ LE +  RL  E  
Sbjct: 471 EELGVQKQRADTLEMELKMLRAQTSSAETSFPFCKEEVDALRLKVEELEGERSRLEQEKQ 530

Query: 572 LLESKLG----HGDFSSTNTKVLRM-VNTLAVDNEAKQTIEA-LKSELQKTKEKLQAVEE 631
            LE ++      GD++ + TKVL M +N  ++  + +Q   A L+ E ++ +  + A+E 
Sbjct: 531 ALEMQMERLTLQGDYNQSRTKVLHMSLNPASMARKRQQEDHARLQGECERLRGLVHALER 590

Query: 632 LKTPSGDAGKLVDSYISGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKI 691
                 D         S ++ +L++Q+ + E + +R K VF  +I  FR+ C  L GY+I
Sbjct: 591 GGPIPADLEVASSLPSSKEVAELRKQVESAELKNQRLKEVFQTKIQEFRKVCYTLTGYQI 650

Query: 692 VMDEHQRADGIPVTRFTLLSIYAQSDDEKLQFEY---ESGNTNILANNYT-SQPELSRQV 726
                   D     ++ L S YA+   + L F+          +L   ++ S PEL   +
Sbjct: 651 --------DVTTENQYRLTSRYAEHQSDCLIFKATGPSGSKMQLLETEFSRSVPEL---I 710

BLAST of HG10006949 vs. ExPASy Swiss-Prot
Match: Q9WTX8 (Mitotic spindle assembly checkpoint protein MAD1 OS=Mus musculus OX=10090 GN=Mad1l1 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.9e-14
Identity = 147/705 (20.85%), Postives = 321/705 (45.53%), Query Frame = 0

Query: 70  DFLDALSNAEKQVHDYELKLGVLTENLSKIESERKKFLDQLLYTEQELAAARGREKVLQE 129
           D   + S + ++ ++Y ++   L E   +I S  K +L Q+   + ++  +  R +V   
Sbjct: 32  DVSTSASGSLQKQYEYHMQ---LEERAEQIRS--KSYLIQVEREKMQMELSHKRARV--- 91

Query: 130 QLIKEINDSGERLKKQMQISSELEVKLQNESNLRMQAESSIALSEEKARLLEDKLNHLSE 189
           +L +  + +    ++++  + EL  +++        AE  +    E+ RL +  L+ +S+
Sbjct: 92  ELERAASTNARNYEREVDRNQELLARIRQLQECEATAEEKMREQLERHRLCKQNLDAVSQ 151

Query: 190 SIERERKRLHSE---LAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKVQLEDLK- 249
            +  +   L S    ++ LKG  ++S  +++A  +K+  +   +EK+    +++L+  K 
Sbjct: 152 QLREQEDSLASAREMISSLKG--RVSELQLSAMDQKVQVKRLESEKQELKEQLELQQRKW 211

Query: 250 -------KQLNECLSQKTELEKKLASFTVNQGTGKESNILIKHLQEELRNYESEVREARK 309
                  ++L     ++ E E+K+          ++   ++K ++ EL       RE ++
Sbjct: 212 QEANQKIQELQASQDERAEHEQKIKDLEQKLCLQEQDAAVVKSMKSELMRMPRMERELKR 271

Query: 310 LKSSLGDI-------GLLKEKLLEEKARRERVESELSNLQEIQLSVKNLEDELTRRELLI 369
           L      +       GLL E+L   + +  R E     L +++L  + L  +L   E L 
Sbjct: 272 LHEENTHLREMKETNGLLTEELEGLQRKLSRQEKMQEALVDLELEKEKLLAKLQSWENLD 331

Query: 370 KDIP-GITTYEDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVVLDAAQIDKQKAESEAT 429
           + +   + T ED+   +  LQ+  +    K   + +  + LE V    Q + ++A ++  
Sbjct: 332 QTMGLNLRTPEDLSRFVVELQQRELTLKEKNNSITSSARGLEKVQQQLQDEVRQANAQLL 391

Query: 430 LVKEKIEALKLEVKQNELLLSVATEERDKLKSLVNELRS-LKNDDAEAKATN-----ETL 489
             ++K E  +   ++ +   ++ T+ERD +++++    S L   +   + T      E +
Sbjct: 392 EERKKRETHEALARRLQKRNALLTKERDGMRAILGSYDSELTQTEYSTQLTQRLWEAEDM 451

Query: 490 SQELDSTLAKKDWYIKELESNLHEQKEVSSRQHDELKLL--------------NERLNGE 549
            Q++ +  ++ +  + +    L  QK+ +     ELK+L               E ++  
Sbjct: 452 VQKVHAHSSEMEAQLSQALEELGVQKQRADTLEMELKMLKAQTSSAESSFSFCKEEVDAL 511

Query: 550 AKRIKSLERDCDRLRSEISLLESKLG----HGDFSSTNTKVLRM-VNTLAVDNEAK-QTI 609
             +++ LE +  RL  E  +LE ++      GD++ + TKVL M +N +++  + + +  
Sbjct: 512 RLKVEELEGERSRLEQEKQVLEMQMEKLTLQGDYNQSRTKVLHMSLNPISMARQRQHEDH 571

Query: 610 EALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYISGKIMQLKEQIATLEKREERYKTVF 669
           + L+ E ++ +  + A+E       D         S ++ +L++Q+ + E + +R K VF
Sbjct: 572 DRLQEECERLRGLVHALERGGPIPADLEAASSLPSSKEVAELRKQVESAELKNQRLKEVF 631

Query: 670 ADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFTLLSIYAQSDDEKLQFEY---ESGN 726
             +I  FR+ C  L GY+I        D    +++ L S YA+   + L F+        
Sbjct: 632 QTKIQEFRKVCYTLTGYQI--------DVTTESQYRLTSRYAEHQTDCLIFKATGPSGSK 691

BLAST of HG10006949 vs. ExPASy Swiss-Prot
Match: Q86RN8 (Paramyosin OS=Rhipicephalus microplus OX=6941 GN=PRM PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 4.3e-05
Identity = 126/601 (20.97%), Postives = 262/601 (43.59%), Query Frame = 0

Query: 79  EKQVHDYELKLGVLTENLSKIESERKKFL---DQLLYTEQELAAARGREKVLQEQLIKEI 138
           E  V++  +++  L   ++++ ++R +      + L    EL  +      L+ QL  ++
Sbjct: 175 EHTVYELNIRIEELNRTVTEVTAQRTRLSAENAEYLKEVHELKVSLDNVNHLKSQLATQL 234

Query: 139 NDSGERL----KKQMQISS----------ELEVKLQNESNLRMQAESSIALSEEKARLLE 198
            D+  RL    +K+  + S           L+V+L+ ES  R++ E  +  +   A   +
Sbjct: 235 EDTRRRLEDEERKRASLESSMHTLEVEIESLKVQLEEESEARLEVERQLVKANADAAAYK 294

Query: 199 DKLNHLSESIERERKRLHSELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKVQL 258
            K        E E +    E+ +L+ +    +S     LE ++ R SN EK+   L+ ++
Sbjct: 295 TK-------YETEVQAHADEVEELRRKMAQKISEYEEQLEALLTRCSNLEKQKSRLQSEV 354

Query: 259 EDLKKQLNECLSQKTELEKKLASF-TVN---QGTGKESNILIKHLQEELRNYESEVR--- 318
           E L   L +  +    LEK++A    +N   +   +E  IL++  Q ELR   +E++   
Sbjct: 355 EVLIMDLEKATAHAQNLEKRVAQLEKLNIDLKSKVEELTILLEQSQRELRQKVAEIQKLQ 414

Query: 319 -EARKLKSSLGDIGLLKEKLLEEKAR-RERVESELSNLQEIQLSVKNLEDELTRRELLIK 378
            E  K++     +    +KL+++ +  + ++   +  L E +L +K LE+E        K
Sbjct: 415 HEYEKMREQRDALQRENKKLVDDLSEAKSQLADAIRRLHEYELEIKRLENERDELAAAYK 474

Query: 379 DIPGITTYED-----IPTKISSLQKEVIDN-TIKMGEVNARLKQLEVVLDAAQIDKQKAE 438
           +   +   E+     +  +++ ++ E      IK  E+ A  KQ +  L+  Q++ + AE
Sbjct: 475 EAETLRKQEEAKCQRLTAELAQVRHEYERRLQIKEEEIEALRKQYQ--LEVEQLNMRLAE 534

Query: 439 SEATL------VKEKIEALKLEVKQNELLLSVATEERDKLKSLV--------------NE 498
           +EA L      +K+K +A   ++ + E+ L  A ++   L+ ++              +E
Sbjct: 535 AEAKLKTEIARIKKKYQA---QITELEMSLDAANKQNMDLQKIIKKQAIQITELQAHYDE 594

Query: 499 LRSLKNDDAEAKATNETLSQELDSTLAKKDWYIKELESNLHEQK------EVSSRQHDEL 558
           +       A+  A ++   Q L + L   D     LES L  ++      E S  + +EL
Sbjct: 595 VHRQLQQCADQLAISQRRCQGLQAEL---DEQRVALESALRSKRAAEQSLEESQARVNEL 654

Query: 559 KLLNERLNGEAKRIKS----LERDCDRLRSEISLLESKLGHGDFSSTNTKVLRMVNTLAV 617
             +N  +     +++S    L+ D D L  E+ +++ +         +TK     + L  
Sbjct: 655 TTINVNIAAAKNKLESELSALQADYDELHKELRVVDERCQRTIVELKSTK-----DILVE 714

BLAST of HG10006949 vs. ExPASy TrEMBL
Match: A0A1S3CCD1 (mitotic spindle checkpoint protein MAD1 OS=Cucumis melo OX=3656 GN=LOC103499375 PE=3 SV=1)

HSP 1 Score: 1255.0 bits (3246), Expect = 0.0e+00
Identity = 683/727 (93.95%), Postives = 705/727 (96.97%), Query Frame = 0

Query: 1   MIVRTPPPKKQRSDVTSLPDSSSAGASDLPLVIYEDPLPLVPATTQPGLSQEPSEHMLCT 60
           MIVRTPPPKKQRSDVTSLPDSS A ASDLPLVIYEDPLPLVPATTQP  S  PS+HMLCT
Sbjct: 1   MIVRTPPPKKQRSDVTSLPDSSPAAASDLPLVIYEDPLPLVPATTQPASSHLPSDHMLCT 60

Query: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGVLTENLSKIESERKKFLDQLLYTEQELAAA 120
           YQCRQMVKSDFLDALSNAEKQVHDYELKLG+L ENLSK+ESERKKFLDQLLYTEQELAAA
Sbjct: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGMLNENLSKVESERKKFLDQLLYTEQELAAA 120

Query: 121 RGREKVLQEQLIKEINDSGERLKKQMQISSELEVKLQNESNLRMQAESSIALSEEKARLL 180
           RGREKVLQEQLIKEI+DSGERLKKQ+Q SSELEVKLQNESNLR++AESSIA SEEKARLL
Sbjct: 121 RGREKVLQEQLIKEISDSGERLKKQLQTSSELEVKLQNESNLRIKAESSIASSEEKARLL 180

Query: 181 EDKLNHLSESIERERKRLHSELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKVQ 240
           EDKLNHLSESIERERK L +ELAQL+GESKLSVSRINADL KMVCRASNAEKESELLK Q
Sbjct: 181 EDKLNHLSESIERERKHLDTELAQLRGESKLSVSRINADLAKMVCRASNAEKESELLKEQ 240

Query: 241 LEDLKKQLNECLSQKTELEKKLASFTVNQGTGKESNILIKHLQEELRNYESEVREARKLK 300
           LEDLKKQLNECL QK+ELEKKLASFTVN+GTGKESNILIKHLQEELRNYESEV+EARKLK
Sbjct: 241 LEDLKKQLNECLCQKSELEKKLASFTVNEGTGKESNILIKHLQEELRNYESEVKEARKLK 300

Query: 301 SSLGDIGLLKEKLLEEKARRERVESELSNLQEIQLSVKNLEDELTRRELLIKDIPGITTY 360
           SSLGDIGLLKEKLLEEKARRER +SELS LQ+IQLSVKNLEDELTRR+LLIKDIPGI+TY
Sbjct: 301 SSLGDIGLLKEKLLEEKARRERADSELSKLQDIQLSVKNLEDELTRRDLLIKDIPGISTY 360

Query: 361 EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVVLDAAQIDKQKAESEATLVKEKIEALK 420
           EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEV LDAAQIDKQKAESEATLVKEKIEALK
Sbjct: 361 EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVALDAAQIDKQKAESEATLVKEKIEALK 420

Query: 421 LEVKQNELLLSVATEERDKLKSLVNELRSLKNDDAEAKATNETLSQELDSTLAKKDWYIK 480
           LEV+QNELLLSVATEERDKLKSLVNEL++LKNDDAEAKATNETLSQELDS  AKKDWYIK
Sbjct: 421 LEVQQNELLLSVATEERDKLKSLVNELKTLKNDDAEAKATNETLSQELDSIFAKKDWYIK 480

Query: 481 ELESNLHEQKEVSSRQHDELKLLNERLNGEAKRIKSLERDCDRLRSEISLLESKLGHGDF 540
           ELESNLHEQKEV+SRQHDELKLLNERLN +AKRIKSLERDCDRLRSEISLLESK+GHGDF
Sbjct: 481 ELESNLHEQKEVNSRQHDELKLLNERLNNDAKRIKSLERDCDRLRSEISLLESKIGHGDF 540

Query: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600
           SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELK PSGDAGKLVDSYIS
Sbjct: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKAPSGDAGKLVDSYIS 600

Query: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660
           GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT
Sbjct: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660

Query: 661 LLSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720
           L SIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES
Sbjct: 661 LQSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720

Query: 721 FNRRTLS 728
           FNRRTLS
Sbjct: 721 FNRRTLS 727

BLAST of HG10006949 vs. ExPASy TrEMBL
Match: A0A6J1EY58 (mitotic spindle checkpoint protein MAD1 OS=Cucurbita moschata OX=3662 GN=LOC111439783 PE=3 SV=1)

HSP 1 Score: 1248.0 bits (3228), Expect = 0.0e+00
Identity = 678/727 (93.26%), Postives = 702/727 (96.56%), Query Frame = 0

Query: 1   MIVRTPPPKKQRSDVTSLPDSSSAGASDLPLVIYEDPLPLVPATTQPGLSQEPSEHMLCT 60
           MIVRTPPPKKQRSDV +LPDSSSAGASDLPLVIYEDPLP+VPATTQP  S EPSE MLCT
Sbjct: 1   MIVRTPPPKKQRSDVRALPDSSSAGASDLPLVIYEDPLPVVPATTQPECSHEPSEQMLCT 60

Query: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGVLTENLSKIESERKKFLDQLLYTEQELAAA 120
           YQCRQMVK+DFLDALSNAEKQVHDYELKLGVL ENLSK+ESERKKFL+QLLYTEQELAAA
Sbjct: 61  YQCRQMVKADFLDALSNAEKQVHDYELKLGVLNENLSKVESERKKFLEQLLYTEQELAAA 120

Query: 121 RGREKVLQEQLIKEINDSGERLKKQMQISSELEVKLQNESNLRMQAESSIALSEEKARLL 180
           RGREKVLQEQLIKEINDSGE+LKKQMQISSELEVKLQNE+NLRM+AESSIALSEEKA+LL
Sbjct: 121 RGREKVLQEQLIKEINDSGEQLKKQMQISSELEVKLQNEANLRMKAESSIALSEEKAKLL 180

Query: 181 EDKLNHLSESIERERKRLHSELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKVQ 240
           EDKLNH SESIERERKRLH ELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLK Q
Sbjct: 181 EDKLNHFSESIERERKRLHGELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKEQ 240

Query: 241 LEDLKKQLNECLSQKTELEKKLASFTVNQGTGKESNILIKHLQEELRNYESEVREARKLK 300
           LEDLKKQLNECL QK+ELEKKLASFTVN+GT KESNILIKHLQEELRNYESEVREARKLK
Sbjct: 241 LEDLKKQLNECLCQKSELEKKLASFTVNEGTSKESNILIKHLQEELRNYESEVREARKLK 300

Query: 301 SSLGDIGLLKEKLLEEKARRERVESELSNLQEIQLSVKNLEDELTRRELLIKDIPGITTY 360
           SSLGDIGLLKEKLLEEKARRER ESEL+ LQ+IQLSVKNLEDELTRRELLIKDIPGITTY
Sbjct: 301 SSLGDIGLLKEKLLEEKARRERAESELTKLQDIQLSVKNLEDELTRRELLIKDIPGITTY 360

Query: 361 EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVVLDAAQIDKQKAESEATLVKEKIEALK 420
           EDIP KISSLQKEVIDNTIKMGEVNARLKQLEV LDAAQ DKQKAESEATL +EKIEAL+
Sbjct: 361 EDIPNKISSLQKEVIDNTIKMGEVNARLKQLEVALDAAQTDKQKAESEATLAREKIEALR 420

Query: 421 LEVKQNELLLSVATEERDKLKSLVNELRSLKNDDAEAKATNETLSQELDSTLAKKDWYIK 480
           LE +QNELLLS+ATEERDKLK+LVNEL+SLKN DAEAKAT+ET+SQELDS LAKKDWYIK
Sbjct: 421 LETQQNELLLSLATEERDKLKNLVNELKSLKNGDAEAKATSETISQELDSALAKKDWYIK 480

Query: 481 ELESNLHEQKEVSSRQHDELKLLNERLNGEAKRIKSLERDCDRLRSEISLLESKLGHGDF 540
           ELESNLHEQK+V+ RQHDEL+LLNERLN EAKRIKSLERD DRLRSEISLLESKLGHGDF
Sbjct: 481 ELESNLHEQKQVNGRQHDELRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKLGHGDF 540

Query: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600
           SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS
Sbjct: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600

Query: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660
           GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR DGIPVTRFT
Sbjct: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRTDGIPVTRFT 660

Query: 661 LLSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720
           L SIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES
Sbjct: 661 LQSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720

Query: 721 FNRRTLS 728
           FNRRTLS
Sbjct: 721 FNRRTLS 727

BLAST of HG10006949 vs. ExPASy TrEMBL
Match: A0A6J1KBD3 (mitotic spindle checkpoint protein MAD1 OS=Cucurbita maxima OX=3661 GN=LOC111492363 PE=3 SV=1)

HSP 1 Score: 1245.3 bits (3221), Expect = 0.0e+00
Identity = 679/727 (93.40%), Postives = 697/727 (95.87%), Query Frame = 0

Query: 1   MIVRTPPPKKQRSDVTSLPDSSSAGASDLPLVIYEDPLPLVPATTQPGLSQEPSEHMLCT 60
           MIVRTPPPKKQRSDV +LPDSSSAGASDLPLVIYEDPLP+VPATTQP  S EPSE MLCT
Sbjct: 1   MIVRTPPPKKQRSDVRALPDSSSAGASDLPLVIYEDPLPVVPATTQPECSHEPSEQMLCT 60

Query: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGVLTENLSKIESERKKFLDQLLYTEQELAAA 120
           YQCRQMVK+DFLDALSNAEKQVHDYELKLG L ENLSK+ESERKKFLDQLLYTEQELAA 
Sbjct: 61  YQCRQMVKADFLDALSNAEKQVHDYELKLGELNENLSKVESERKKFLDQLLYTEQELAAT 120

Query: 121 RGREKVLQEQLIKEINDSGERLKKQMQISSELEVKLQNESNLRMQAESSIALSEEKARLL 180
           RGREKVLQEQLIKEINDSGE+LKKQMQISSELEVKLQ E+NLRM+AESSIALSEEKA+LL
Sbjct: 121 RGREKVLQEQLIKEINDSGEQLKKQMQISSELEVKLQKEANLRMKAESSIALSEEKAKLL 180

Query: 181 EDKLNHLSESIERERKRLHSELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKVQ 240
           EDKLNH SESIERERKRLH ELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLK Q
Sbjct: 181 EDKLNHFSESIERERKRLHGELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKEQ 240

Query: 241 LEDLKKQLNECLSQKTELEKKLASFTVNQGTGKESNILIKHLQEELRNYESEVREARKLK 300
           LEDLKKQLNECL QK+ELEKKLASFTVN+GT KESNILIKHLQEELRNYE EVREARKLK
Sbjct: 241 LEDLKKQLNECLCQKSELEKKLASFTVNEGTSKESNILIKHLQEELRNYEFEVREARKLK 300

Query: 301 SSLGDIGLLKEKLLEEKARRERVESELSNLQEIQLSVKNLEDELTRRELLIKDIPGITTY 360
           SSLGDIGLLKEKLLEEKARRER ESELS LQ+IQLSVKNLEDELTRRELLIKDIPGITTY
Sbjct: 301 SSLGDIGLLKEKLLEEKARRERAESELSKLQDIQLSVKNLEDELTRRELLIKDIPGITTY 360

Query: 361 EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVVLDAAQIDKQKAESEATLVKEKIEALK 420
           EDIP KISSLQKEVIDNTIKMGEVNARLKQLEV LDAAQ DKQKAESEATL KEKIEALK
Sbjct: 361 EDIPNKISSLQKEVIDNTIKMGEVNARLKQLEVALDAAQTDKQKAESEATLAKEKIEALK 420

Query: 421 LEVKQNELLLSVATEERDKLKSLVNELRSLKNDDAEAKATNETLSQELDSTLAKKDWYIK 480
           LE +QNELLLSVATEERDKLKSLVNEL+SLKN DAEAK T+ET+SQELDS LAKKDWYIK
Sbjct: 421 LETQQNELLLSVATEERDKLKSLVNELKSLKNGDAEAKTTSETISQELDSALAKKDWYIK 480

Query: 481 ELESNLHEQKEVSSRQHDELKLLNERLNGEAKRIKSLERDCDRLRSEISLLESKLGHGDF 540
           ELESNLHEQK+V+ RQHDEL+LLNERLN EAKRIKSLERD DRLRSEISLLESKLGHGDF
Sbjct: 481 ELESNLHEQKQVNGRQHDELRLLNERLNNEAKRIKSLERDSDRLRSEISLLESKLGHGDF 540

Query: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600
           SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS
Sbjct: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600

Query: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660
           GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR DGIPVTRFT
Sbjct: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRTDGIPVTRFT 660

Query: 661 LLSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720
           L SIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES
Sbjct: 661 LQSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720

Query: 721 FNRRTLS 728
           FNRRTLS
Sbjct: 721 FNRRTLS 727

BLAST of HG10006949 vs. ExPASy TrEMBL
Match: A0A0A0KV95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G630750 PE=3 SV=1)

HSP 1 Score: 1198.0 bits (3098), Expect = 0.0e+00
Identity = 653/699 (93.42%), Postives = 674/699 (96.42%), Query Frame = 0

Query: 1   MIVRTPPPKKQRSDVTSLPDSSSAGASDLPLVIYEDPLPLVPATTQPGLSQEPSEHMLCT 60
           MIVRTPPPKKQRSDVTSLPDSS A ASDLPLVIYEDPLPLVPATT+P  S EPS+HMLCT
Sbjct: 1   MIVRTPPPKKQRSDVTSLPDSSPAAASDLPLVIYEDPLPLVPATTEPASSHEPSDHMLCT 60

Query: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGVLTENLSKIESERKKFLDQLLYTEQELAAA 120
           YQCRQMVKSDFLDALSNAEKQVHDYELKLGVL ENLSK+ESERKKFLDQLLYTEQELAAA
Sbjct: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGVLNENLSKVESERKKFLDQLLYTEQELAAA 120

Query: 121 RGREKVLQEQLIKEINDSGERLKKQMQISSELEVKLQNESNLRMQAESSIALSEEKARLL 180
           R REKVLQEQLIKEI+DSGERLKKQMQISSELEV+LQNESNLR++AESSIA SEEKARLL
Sbjct: 121 RRREKVLQEQLIKEISDSGERLKKQMQISSELEVRLQNESNLRIKAESSIASSEEKARLL 180

Query: 181 EDKLNHLSESIERERKRLHSELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKVQ 240
           EDKLNHLSESIERERK L +ELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLK Q
Sbjct: 181 EDKLNHLSESIERERKHLDTELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKGQ 240

Query: 241 LEDLKKQLNECLSQKTELEKKLASFTVNQGTGKESNILIKHLQEELRNYESEVREARKLK 300
           LEDLK QLNECL QK+ELEKKLASFTVN+GTGKESNILIKHLQEELRNYESEV+EARKLK
Sbjct: 241 LEDLKNQLNECLCQKSELEKKLASFTVNEGTGKESNILIKHLQEELRNYESEVKEARKLK 300

Query: 301 SSLGDIGLLKEKLLEEKARRERVESELSNLQEIQLSVKNLEDELTRRELLIKDIPGITTY 360
           SSLGDIGLLKEKLLEEKARRER +SELS LQ+IQLSVKNLEDELTRR+LLI  IPGI+TY
Sbjct: 301 SSLGDIGLLKEKLLEEKARRERADSELSKLQDIQLSVKNLEDELTRRDLLINSIPGISTY 360

Query: 361 EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVVLDAAQIDKQKAESEATLVKEKIEALK 420
           EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEV LDAAQIDKQKAESEATLV+EKIEALK
Sbjct: 361 EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVALDAAQIDKQKAESEATLVEEKIEALK 420

Query: 421 LEVKQNELLLSVATEERDKLKSLVNELRSLKNDDAEAKATNETLSQELDSTLAKKDWYIK 480
           LEVKQNELLLSVATEERDKLKSLVNEL++LKNDDAEAK T ETLSQELD TLAKKDWYIK
Sbjct: 421 LEVKQNELLLSVATEERDKLKSLVNELKTLKNDDAEAKETKETLSQELDLTLAKKDWYIK 480

Query: 481 ELESNLHEQKEVSSRQHDELKLLNERLNGEAKRIKSLERDCDRLRSEISLLESKLGHGDF 540
           ELESNLHEQKEV+SRQHDELKLLNERLN +AKRIKSLERDCDRLRSEISLLESK+GHGDF
Sbjct: 481 ELESNLHEQKEVNSRQHDELKLLNERLNNDAKRIKSLERDCDRLRSEISLLESKIGHGDF 540

Query: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600
           SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELK PSGDAGKLVDSYIS
Sbjct: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKAPSGDAGKLVDSYIS 600

Query: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660
           GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT
Sbjct: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660

Query: 661 LLSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQV 700
           L SIYAQSDDEKLQFEYESGNTNIL NNYTSQPELSRQV
Sbjct: 661 LQSIYAQSDDEKLQFEYESGNTNILVNNYTSQPELSRQV 699

BLAST of HG10006949 vs. ExPASy TrEMBL
Match: A0A6J1DLL9 (mitotic spindle checkpoint protein MAD1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022291 PE=3 SV=1)

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 630/727 (86.66%), Postives = 679/727 (93.40%), Query Frame = 0

Query: 1   MIVRTPPPKKQRSDVTSLPDSSSAGASDLPLVIYEDPLPLVPATTQPGLSQEPSEHMLCT 60
           MI+RTPP KKQRSDV +LPD+SS  ASDLPLVIYEDPLP +P T+QP LS   SE MLCT
Sbjct: 1   MILRTPPLKKQRSDVRALPDASSGAASDLPLVIYEDPLPSLPPTSQPELSHNHSEPMLCT 60

Query: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGVLTENLSKIESERKKFLDQLLYTEQELAAA 120
           YQCRQMVKSDFLDALSNAEKQVHDYELKLG + ENLSK E ERKK+LDQLLYTEQELAA+
Sbjct: 61  YQCRQMVKSDFLDALSNAEKQVHDYELKLGTVNENLSKSEVERKKYLDQLLYTEQELAAS 120

Query: 121 RGREKVLQEQLIKEINDSGERLKKQMQISSELEVKLQNESNLRMQAESSIALSEEKARLL 180
           RGREKVLQEQLIKE+NDSGE+LKKQ+QI+SELE+KLQNESNLR +AESSIA SEEKA LL
Sbjct: 121 RGREKVLQEQLIKEVNDSGEQLKKQIQINSELEIKLQNESNLRTKAESSIASSEEKASLL 180

Query: 181 EDKLNHLSESIERERKRLHSELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKVQ 240
           EDKLNHLS+SIERE+KRLH+ELAQLKGESKLSVSRI+ADLEKMVCRASNAEKESELLK Q
Sbjct: 181 EDKLNHLSQSIEREKKRLHNELAQLKGESKLSVSRISADLEKMVCRASNAEKESELLKEQ 240

Query: 241 LEDLKKQLNECLSQKTELEKKLASFTVNQGTGKESNILIKHLQEELRNYESEVREARKLK 300
           LEDLKKQLNECLSQK+ELEKKLAS TVN+GT KESNILIKHLQEELRN ESEVREARKLK
Sbjct: 241 LEDLKKQLNECLSQKSELEKKLASLTVNEGTSKESNILIKHLQEELRNCESEVREARKLK 300

Query: 301 SSLGDIGLLKEKLLEEKARRERVESELSNLQEIQLSVKNLEDELTRRELLIKDIPGITTY 360
           SSL DIGLLKEKLLEEKARR R E+ELS LQ++QLSVK LEDEL R ELLIKDIPGI TY
Sbjct: 301 SSLSDIGLLKEKLLEEKARRGRAEAELSKLQDLQLSVKKLEDELARNELLIKDIPGIKTY 360

Query: 361 EDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVVLDAAQIDKQKAESEATLVKEKIEALK 420
           ED+P KISSLQKEVID+TIK+GEV+ARLKQLEV LD AQIDKQKAE+EATL KEKIEALK
Sbjct: 361 EDLPNKISSLQKEVIDSTIKVGEVSARLKQLEVALDTAQIDKQKAETEATLAKEKIEALK 420

Query: 421 LEVKQNELLLSVATEERDKLKSLVNELRSLKNDDAEAKATNETLSQELDSTLAKKDWYIK 480
           LE KQNELLL + TEERDKLKSLV+EL+ LKNDD  ++  +E LSQ+LDS LAKKDWYIK
Sbjct: 421 LEAKQNELLLPMVTEERDKLKSLVSELKGLKNDDTRSQVASENLSQDLDSALAKKDWYIK 480

Query: 481 ELESNLHEQKEVSSRQHDELKLLNERLNGEAKRIKSLERDCDRLRSEISLLESKLGHGDF 540
           ELESNL EQ EV+++QH+E++LLNERLN EAKRI+SLERDCDRLRSEISLLESKLGHGDF
Sbjct: 481 ELESNLREQTEVNNQQHEEIRLLNERLNNEAKRIRSLERDCDRLRSEISLLESKLGHGDF 540

Query: 541 SSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYIS 600
           S+T+TKVLRMVNTLAVDNEAKQTI+ALKSELQKTKEKLQAVEELKTPSGDAGK+VDSYIS
Sbjct: 541 SATSTKVLRMVNTLAVDNEAKQTIDALKSELQKTKEKLQAVEELKTPSGDAGKVVDSYIS 600

Query: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660
           GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT
Sbjct: 601 GKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRFT 660

Query: 661 LLSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVES 720
           L SIYAQSD+EKLQFEYESGNTNILAN+YTSQPELSRQ+EIFIRKMNSIPAFTANLTVES
Sbjct: 661 LQSIYAQSDEEKLQFEYESGNTNILANSYTSQPELSRQIEIFIRKMNSIPAFTANLTVES 720

Query: 721 FNRRTLS 728
           FNRRTLS
Sbjct: 721 FNRRTLS 727

BLAST of HG10006949 vs. TAIR 10
Match: AT5G49880.1 (mitotic checkpoint family protein )

HSP 1 Score: 818.5 bits (2113), Expect = 4.2e-237
Identity = 459/727 (63.14%), Postives = 578/727 (79.50%), Query Frame = 0

Query: 1   MIVRTPPPKKQRSDVTSLPDSSSAGASDLPLVIYED-PLPLVPATTQPGLSQEPSEHMLC 60
           MI+RTP PK+ RSD    P  + A  S   L+IYED PLP  PA  Q        +H LC
Sbjct: 1   MILRTPQPKRLRSDAGESPFPTGATGSGNQLIIYEDSPLP-APAPLQTSHDHSADQH-LC 60

Query: 61  TYQCRQMVKSDFLDALSNAEKQVHDYELKLGVLTENLSKIESERKKFLDQLLYTEQELAA 120
           TYQCRQMVK+D LDALS AEKQV + + KL  L  N ++ ++ERK F D+ LY+EQELAA
Sbjct: 61  TYQCRQMVKADVLDALSTAEKQVEESKTKLQTLNANFTEADAERKHFRDKFLYSEQELAA 120

Query: 121 ARGREKVLQEQLIKEINDSGERLKKQMQISSELEVKLQNESNLRMQAESSIALSEEKARL 180
           A+ REK+LQEQL+ EIN+S ER  K++Q   ELEVKLQNE NLR +AESS A +EEKA+L
Sbjct: 121 AKAREKMLQEQLLMEINNSQERYTKELQSCHELEVKLQNEMNLRKKAESSAATAEEKAKL 180

Query: 181 LEDKLNHLSESIERERKRLHSELAQLKGESKLSVSRINADLEKMVCRASNAEKESELLKV 240
           LEDKL  LS S++RE+KRL++++AQL  E+KLSV+RI ADLE+M CRA NAE ES LL+ 
Sbjct: 181 LEDKLTQLSGSVDREKKRLNNDIAQLGKEAKLSVARIGADLERMQCRAQNAETESNLLRS 240

Query: 241 QLEDLKKQLNECLSQKTELEKKLASFTVNQGTGKESNILIKHLQEELRNYESEVREARKL 300
           QLE LK   +ECL +KTE++KKL+SFT    +  ++++L+KHLQEEL+ YE+EVREARKL
Sbjct: 241 QLEHLKLIFDECLQEKTEVDKKLSSFTSEAASSSDNSVLVKHLQEELKRYEAEVREARKL 300

Query: 301 KSSLGDIGLLKEKLLEEKARRERVESELSNLQEIQLSVKNLEDELTRRELLIKDIPGITT 360
           KS   D  LL   LLEE++RRER ESELS   ++QLS++ LE+EL+  + L+ DIPG++ 
Sbjct: 301 KSRHLDAELLNVNLLEEQSRRERAESELSKFHDLQLSMEKLENELSSWKSLLNDIPGVSC 360

Query: 361 YEDIPTKISSLQKEVIDNTIKMGEVNARLKQLEVVLDAAQIDKQKAESEATLVKEKIEAL 420
            +DI  + S LQ EV+ +T+K+GE + R+KQLE  L+A Q+ +Q A SEA L KEK EAL
Sbjct: 361 PDDIVMRFSVLQNEVVQSTMKIGEASTRIKQLEETLEAIQLGRQNAVSEAALAKEKSEAL 420

Query: 421 KLEVKQNELLLSVATEERDKLKSLVNELRSLKNDDAEAKATNETLSQELDSTLAKKDWYI 480
           K +VK+ E++L++ TEE+++LK++VNELR   ++ + + A +  L Q  +S+LAKK+ YI
Sbjct: 421 KTDVKRIEVMLTLVTEEKEQLKAVVNELRKSNSEGSVSGAADGALIQGFESSLAKKENYI 480

Query: 481 KELESNLHEQKEVSSRQHDELKLLNERLNGEAKRIKSLERDCDRLRSEISLLESKLGHGD 540
           K+LE +L++ K+V++RQ  E++LLNE+L  EA+R KSLERD DRLRSEISLLESKLGHGD
Sbjct: 481 KDLEQDLNQLKDVNNRQRTEIELLNEKLVDEARRNKSLERDSDRLRSEISLLESKLGHGD 540

Query: 541 FSSTNTKVLRMVNTLAVDNEAKQTIEALKSELQKTKEKLQAVEELKTPSGDAGKLVDSYI 600
           +S+ NT+VLRMVNTL V+NEAKQTIEAL++ELQKTKE+LQAVEELK+ SGDAGKLVDS+I
Sbjct: 541 YSAANTRVLRMVNTLGVENEAKQTIEALQAELQKTKERLQAVEELKSQSGDAGKLVDSHI 600

Query: 601 SGKIMQLKEQIATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRADGIPVTRF 660
           +GKI QLKEQ ATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQR +GIPVTRF
Sbjct: 601 TGKIAQLKEQNATLEKREERYKTVFADRISVFRRACCELFGYKIVMDEHQRPNGIPVTRF 660

Query: 661 TLLSIYAQSDDEKLQFEYESGNTNILANNYTSQPELSRQVEIFIRKMNSIPAFTANLTVE 720
           TL SIYAQSDDEKL+FEYESGNT+IL N Y SQ ++++Q+EIFIRK NSIPAFTANLT+E
Sbjct: 661 TLQSIYAQSDDEKLEFEYESGNTSILNNEYASQGDIAKQIEIFIRKFNSIPAFTANLTME 720

Query: 721 SFNRRTL 727
           SFNRRTL
Sbjct: 721 SFNRRTL 725