HG10006810 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10006810
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Mediator of RNA polymerase II transcription subunit 20
Location	Chr07: 22206421 .. 22208370 (-)
RNA-Seq Expression	HG10006810
Synteny	HG10006810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGCTAAAATGGTAAAACCCCAGAAACCCCAATCAAAAAACTTCTCATCCCTTTTTGTTCATTTGATTCTTGATCTACTTTCATCGTTTTCCTTTGCTTAGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACGGTTAACAGTCAGATACTCAACGAGGTTTCGCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGAAAATGGAAAGCTACTCTCACTTTCTACAAACCCATCTTACGAGGTAATCGCACTCTTTTTCTTCAGACGTTTTTCGGAATTTGATTACCTAAAATGAATCTTAAATCTGTCAACAGATCAAAACTTACCAGCGGCCGAGTTCCCGCGGGATTTTCTCGGAATTTCGCTGCCGGAGCAACCAAACAAGTATTACCTTATAATTCGTGGCCAACGGATCGTCTTGGAAGCTGATTTCACGATTCAGGCTATAATGGAGAAGCTGCAGTCTTACAAGTTGCGCGTTGCTCTCAATTTCGAGGTTGACCACTCAACTTCTCTAGTTCTGATGTACTCTGCATCTTTCCCATGTCCTGACATTCAAATTTGAAGTTGATTTTTGTGGCTTTCTAGTCTCTACTCTTTTGCCCTTTTTGTTGATAAATCTTTGATTACAGACAGTTCACATTATTGGTTTTTTAGGGGTTCCAGTATCAGTTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAACAATTCCGAGAGTTTGAGAGGAATAGTTATGGAGGTAAGGCTCATTTCATCATTTCATGTCAACTCTGTTCTTGTGTTATTGCATGTTCTTCTGGTTGTTTGTTTTGTTTGTTTTTTCATATCTTTTTCTTTATCTGAGAGAGCAGATTCTTTTCCATGTGCTATTTCAACACAATGTTTGGCCTGTGTTCTCATCTTCAAAACAATGCGTTGTAGTTTGGATATAATTAAATTTACCATAATCTAATAAGTTTAAAGCTTTTGATTTCATTTAACATAGTATCAAGGTAAGAGGTAAGAGGTCCCTTGTTTGAACTATTGAAATATCATTTACTCCTGAAATTAATATTTGATGTTCACTTGTTGGGATTTGTAGTGTTGATAAGCTGCTTGGTTCATTGATGATTTAACACAATAGAGTAAAGAAAAAAAAAATCAGTCATCATAATTCTCTGAAAAACTTATCAGTGAGCTTTGGAGTGAGGGATCTTCTGTTGAGAAGTAGTGAGATCTGTTTAGGATATCTTTGCAAAAGCCTATGTAGGATTAGTTATGGAAGTCTTTAAAGTGATCTTCACATTTCTTAATATTTCATACTCATTGTTGCACATGAATCAAAGTCTTTCGAGTAAATTCCTCTGAAAGAGTCATTTAGTTTGGAAAATGTCTTCCACTTCACTTGAATGCTTGATGAGAATGTGAATGTTTAGTAGTCTTTTCTTAATCCTGATATAGGTAAAGTAAAGAAAGATGCAGATTTCAGATGTCTGTCCTTCATTATCTACCAGATCTTGATTCTGTTTTGTGGTTTGTTTTTAATTGGTACGATGAACATCATATTGGAAGTATGGTTAAATCAAGTGTGGCCATGTTCTATTTTTTGGGTGTATATATATATATATGAAAATTGCTCTTTGTTGGTGTTCGAATGAAACATTTTGAACTCTTGATAGAGATTCAATAGTGTATTAAAACCTGTATATTGCAGATTGAGTATCTCCCAATTTCTTCAATGGAGAAATCAAGACAGGTAATGGAGGAGTTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCATTACCAGGTCGTTTCATGCACATAGAACCAAACTTTGCAGAATATGGACTGTCGGATTACTACACTTCTCAACACACAGCAGTTCAATATGGCAACGTGATGGCTCAACTAATCGCTACTGTGCAAGCAGTTCAATCAGCAAGAAATTAG

mRNA sequence

ATGCCGCTAAAATGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACGGTTAACAGTCAGATACTCAACGAGGTTTCGCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGAAAATGGAAAGCTACTCTCACTTTCTACAAACCCATCTTACGAGATCAAAACTTACCAGCGGCCGAGTTCCCGCGGGATTTTCTCGGAATTTCGCTGCCGGAGCAACCAAACAAGTATTACCTTATAATTCGTGGCCAACGGATCGTCTTGGAAGCTGATTTCACGATTCAGGCTATAATGGAGAAGCTGCAGTCTTACAAGTTGCGCGTTGCTCTCAATTTCGAGGGGTTCCAGTATCAGTTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAACAATTCCGAGAGTTTGAGAGGAATAGTTATGGAGATTGAGTATCTCCCAATTTCTTCAATGGAGAAATCAAGACAGGTAATGGAGGAGTTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCATTACCAGGTCGTTTCATGCACATAGAACCAAACTTTGCAGAATATGGACTGTCGGATTACTACACTTCTCAACACACAGCAGTTCAATATGGCAACGTGATGGCTCAACTAATCGCTACTGTGCAAGCAGTTCAATCAGCAAGAAATTAG

Coding sequence (CDS)

ATGCCGCTAAAATGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACGGTTAACAGTCAGATACTCAACGAGGTTTCGCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGAAAATGGAAAGCTACTCTCACTTTCTACAAACCCATCTTACGAGATCAAAACTTACCAGCGGCCGAGTTCCCGCGGGATTTTCTCGGAATTTCGCTGCCGGAGCAACCAAACAAGTATTACCTTATAATTCGTGGCCAACGGATCGTCTTGGAAGCTGATTTCACGATTCAGGCTATAATGGAGAAGCTGCAGTCTTACAAGTTGCGCGTTGCTCTCAATTTCGAGGGGTTCCAGTATCAGTTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAACAATTCCGAGAGTTTGAGAGGAATAGTTATGGAGATTGAGTATCTCCCAATTTCTTCAATGGAGAAATCAAGACAGGTAATGGAGGAGTTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCATTACCAGGTCGTTTCATGCACATAGAACCAAACTTTGCAGAATATGGACTGTCGGATTACTACACTTCTCAACACACAGCAGTTCAATATGGCAACGTGATGGCTCAACTAATCGCTACTGTGCAAGCAGTTCAATCAGCAAGAAATTAG

Protein sequence

MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAEFPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN

Homology

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BLAST of HG10006810 vs. NCBI nr
Match: XP_038889134.1 (mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Benincasa hispida])

HSP 1 Score: 445.7 bits (1145), Expect = 2.4e-121
Identity = 220/223 (98.65%), Postives = 222/223 (99.55%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSE+LRGIVMEIEYLPISSMEKSR+VMEEFFEVWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRKVMEEFFEVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 224
           HIEPNFAEYGLSDYYTSQHTAVQY NVMAQLIATVQAVQSARN
Sbjct: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of HG10006810 vs. NCBI nr
Match: XP_004141069.1 (mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60509.1 hypothetical protein Csa_019466 [Cucumis sativus])

HSP 1 Score: 443.0 bits (1138), Expect = 1.6e-120
Identity = 219/223 (98.21%), Postives = 221/223 (99.10%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN PAAE
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSE+LRGIVMEIEYLPISSMEKSRQVMEEFF+VWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 224
           HIE NFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN
Sbjct: 181 HIESNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of HG10006810 vs. NCBI nr
Match: XP_008459711.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo])

HSP 1 Score: 438.0 bits (1125), Expect = 5.0e-119
Identity = 215/223 (96.41%), Postives = 220/223 (98.65%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN PAAE
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSE+LRGIVMEIEYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 224
           HIE NFAEYGLSD+YT QHTAVQYGNVMAQLIATVQAVQSARN
Sbjct: 181 HIESNFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of HG10006810 vs. NCBI nr
Match: KAA0061469.1 (mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo var. makuwa] >TYK10805.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 438.0 bits (1125), Expect = 5.0e-119
Identity = 215/223 (96.41%), Postives = 220/223 (98.65%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN PAAE
Sbjct: 49  MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 108

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 109 FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 168

Query: 121 DFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSE+LRGIVMEIEYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRFM
Sbjct: 169 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFM 228

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 224
           HIE NFAEYGLSD+YT QHTAVQYGNVMAQLIATVQAVQSARN
Sbjct: 229 HIESNFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 271

[Close]

BLAST of HG10006810 vs. NCBI nr
Match: XP_022946745.1 (mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata] >XP_022999230.1 mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita maxima] >XP_023546929.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546930.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X2 [Cucurbita pepo subsp. pepo] >KAG6599532.1 Mediator of RNA polymerase II transcription subunit 20a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 437.2 bits (1123), Expect = 8.6e-119
Identity = 215/223 (96.41%), Postives = 219/223 (98.21%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N+ A E
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSE+LRGIVMEIEYLPISSMEKS+QVMEEFFEVWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 224
           HIEPNFAEYGLSDYYTSQHTAVQY NVMAQLIATVQAVQSARN
Sbjct: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of HG10006810 vs. ExPASy Swiss-Prot
Match: Q6NPF4 (Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana OX=3702 GN=MED20A PE=1 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 1.1e-89
Identity = 160/219 (73.06%), Postives = 191/219 (87.21%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MP+KWVLHWQPN GSTV+SQILNE +QCVESI+GVK G+WKATL +YKP+L+DQ     E
Sbjct: 1   MPVKWVLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKDQ-ANQLE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISL +QPNKYY IIR QRIVLEAD +IQ IMEKLQSYK +VAL F+GFQYQLG
Sbjct: 61  FPRDFLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DF+LRVGKVVP +SE++RGIVME+EYLPISSMEK+++VMEEF E+W EA++K SLPG+F+
Sbjct: 121 DFRLRVGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFV 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQ 220
           +I+ NF E+GL D YT QHTAV+Y  VMA +IATVQAV+
Sbjct: 181 NIDLNFGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVR 218

[Close]

BLAST of HG10006810 vs. ExPASy Swiss-Prot
Match: Q9M0S0 (Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana OX=3702 GN=MED20B PE=3 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.3e-80
Identity = 141/217 (64.98%), Postives = 181/217 (83.41%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MP+KW+LHWQPN GST +SQILNEV+Q +ES++GVK G WKATL +YKP+L+DQ    AE
Sbjct: 1   MPVKWLLHWQPNQGSTFSSQILNEVTQSIESLNGVKEGTWKATLNYYKPMLQDQ-ANQAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPR+F+GISLPE+P+KYY +IR QRIV+EAD +IQ IME LQSYK +++  FEG +YQLG
Sbjct: 61  FPREFVGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFEGLEYQLG 120

Query: 121 DFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DF+LRVGKVVP ++E++RG+VME+EYLPISSM  ++++MEEF E+WQEAMSK SLPG+F+
Sbjct: 121 DFRLRVGKVVPTHAETIRGVVMEVEYLPISSMGMAKKLMEEFLEIWQEAMSKRSLPGKFV 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQA 218
           + E NF ++GL D YT QHTAV Y   MA L+A +QA
Sbjct: 181 NKELNFEKFGLGDNYTPQHTAVGYAFFMANLMAAIQA 216

[Close]

BLAST of HG10006810 vs. ExPASy Swiss-Prot
Match: Q54FL3 (Putative mediator of RNA polymerase II transcription subunit 20 OS=Dictyostelium discoideum OX=44689 GN=med20 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 7.5e-09
Identity = 56/197 (28.43%), Postives = 92/197 (46.70%), Query Frame = 0


Query: 22  LNEVSQCVESIHGVKGGKWKATLTFYKPILRD-QNLPAAEFPRDFLGISLPEQPNKYYLI 81
           L E+++ +E + G+K        + Y   + D  NL     PR+F  +   E+P K + +
Sbjct: 13  LPEMTKRIEFLGGIKVSTGTINCSLYLERVPDGSNL----IPREFHLLLYDEKPKKCFTV 72

Query: 82  IRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPNNSESLRGI 141
            R    ++E D  + +I+EK  SYK R        +Y+LGDF +RVG +V      +RGI
Sbjct: 73  CRD--TIVETDREMVSILEKTNSYKKRQTTEALISKYELGDFIIRVGPIVFRT--EIRGI 132

Query: 142 VMEIEY----LPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDY-- 201
           ++E+EY     P++S     +++ EF          +   G F   EP    +  S Y  
Sbjct: 133 LVEVEYTACSTPVTSPFNCTKLLSEFI---------NGNLGPFP--EPIQTSFDFSAYPT 190

Query: 202 ----YTSQHTAVQYGNV 208
               Y+  HT+ QY N+
Sbjct: 193 LPKSYSDLHTSYQYVNL 190

[Close]

BLAST of HG10006810 vs. ExPASy TrEMBL
Match: A0A0A0LHQ1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=3659 GN=MED20 PE=3 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 7.6e-121
Identity = 219/223 (98.21%), Postives = 221/223 (99.10%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN PAAE
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSE+LRGIVMEIEYLPISSMEKSRQVMEEFF+VWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 224
           HIE NFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN
Sbjct: 181 HIESNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of HG10006810 vs. ExPASy TrEMBL
Match: A0A5D3CH02 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. makuwa OX=1194695 GN=MED20 PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 2.4e-119
Identity = 215/223 (96.41%), Postives = 220/223 (98.65%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN PAAE
Sbjct: 49  MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 108

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 109 FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 168

Query: 121 DFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSE+LRGIVMEIEYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRFM
Sbjct: 169 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFM 228

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 224
           HIE NFAEYGLSD+YT QHTAVQYGNVMAQLIATVQAVQSARN
Sbjct: 229 HIESNFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 271

[Close]

BLAST of HG10006810 vs. ExPASy TrEMBL
Match: A0A1S3CAW6 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 GN=LOC103498750 PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 2.4e-119
Identity = 215/223 (96.41%), Postives = 220/223 (98.65%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN PAAE
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSE+LRGIVMEIEYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 224
           HIE NFAEYGLSD+YT QHTAVQYGNVMAQLIATVQAVQSARN
Sbjct: 181 HIESNFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of HG10006810 vs. ExPASy TrEMBL
Match: A0A6J1KEU1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=3661 GN=LOC111493666 PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 4.2e-119
Identity = 215/223 (96.41%), Postives = 219/223 (98.21%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N+ A E
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSE+LRGIVMEIEYLPISSMEKS+QVMEEFFEVWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 224
           HIEPNFAEYGLSDYYTSQHTAVQY NVMAQLIATVQAVQSARN
Sbjct: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of HG10006810 vs. ExPASy TrEMBL
Match: A0A6J1G4U3 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=3662 GN=LOC111450720 PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 4.2e-119
Identity = 215/223 (96.41%), Postives = 219/223 (98.21%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N+ A E
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPNNSE+LRGIVMEIEYLPISSMEKS+QVMEEFFEVWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 224
           HIEPNFAEYGLSDYYTSQHTAVQY NVMAQLIATVQAVQSARN
Sbjct: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of HG10006810 vs. TAIR 10
Match: AT2G28230.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 330.9 bits (847), Expect = 8.1e-91
Identity = 160/219 (73.06%), Postives = 191/219 (87.21%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MP+KWVLHWQPN GSTV+SQILNE +QCVESI+GVK G+WKATL +YKP+L+DQ     E
Sbjct: 1   MPVKWVLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKDQ-ANQLE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISL +QPNKYY IIR QRIVLEAD +IQ IMEKLQSYK +VAL F+GFQYQLG
Sbjct: 61  FPRDFLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQLG 120

Query: 121 DFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DF+LRVGKVVP +SE++RGIVME+EYLPISSMEK+++VMEEF E+W EA++K SLPG+F+
Sbjct: 121 DFRLRVGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFV 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQ 220
           +I+ NF E+GL D YT QHTAV+Y  VMA +IATVQAV+
Sbjct: 181 NIDLNFGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVR 218

[Close]

BLAST of HG10006810 vs. TAIR 10
Match: AT4G09070.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 300.8 bits (769), Expect = 8.9e-82
Identity = 141/217 (64.98%), Postives = 181/217 (83.41%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60
           MP+KW+LHWQPN GST +SQILNEV+Q +ES++GVK G WKATL +YKP+L+DQ    AE
Sbjct: 1   MPVKWLLHWQPNQGSTFSSQILNEVTQSIESLNGVKEGTWKATLNYYKPMLQDQ-ANQAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120
           FPR+F+GISLPE+P+KYY +IR QRIV+EAD +IQ IME LQSYK +++  FEG +YQLG
Sbjct: 61  FPREFVGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFEGLEYQLG 120

Query: 121 DFQLRVGKVVPNNSESLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DF+LRVGKVVP ++E++RG+VME+EYLPISSM  ++++MEEF E+WQEAMSK SLPG+F+
Sbjct: 121 DFRLRVGKVVPTHAETIRGVVMEVEYLPISSMGMAKKLMEEFLEIWQEAMSKRSLPGKFV 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQA 218
           + E NF ++GL D YT QHTAV Y   MA L+A +QA
Sbjct: 181 NKELNFEKFGLGDNYTPQHTAVGYAFFMANLMAAIQA 216

[Close]

BLAST of HG10006810 vs. TAIR 10
Match: AT2G28020.1 (BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of subunit 20 of Mediator complex (TAIR:AT2G28230.1); Has 37 Blast hits to 37 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 37; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 82.8 bits (203), Expect = 3.8e-16
Identity = 42/68 (61.76%), Postives = 54/68 (79.41%), Query Frame = 0


Query: 152 MEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQ-YGNVMAQ 211
           ME +R+VMEEF E+W EA++K SLPG+F++ + NF E+GL D YT QHTAV+ Y  VMA 
Sbjct: 1   MEIARKVMEEFLEIWNEALAKRSLPGKFVNKDLNFGEFGLGDNYTPQHTAVRNYALVMAH 60

Query: 212 LIATVQAV 219
           +IATVQAV
Sbjct: 61  MIATVQAV 68

The following BLAST results are available for this feature:

BLAST of HG10006810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889134.1	2.4e-121	98.65	mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Beninca...	[more]
XP_004141069.1	1.6e-120	98.21	mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60...	[more]
XP_008459711.1	5.0e-119	96.41	PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform ...	[more]
KAA0061469.1	5.0e-119	96.41	mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis...	[more]
XP_022946745.1	8.6e-119	96.41	mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata...	[more]

BLAST of HG10006810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NPF4	1.1e-89	73.06	Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana ...	[more]
Q9M0S0	1.3e-80	64.98	Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana ...	[more]
Q54FL3	7.5e-09	28.43	Putative mediator of RNA polymerase II transcription subunit 20 OS=Dictyostelium...	[more]

BLAST of HG10006810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHQ1	7.6e-121	98.21	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=365...	[more]
A0A5D3CH02	2.4e-119	96.41	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. maku...	[more]
A0A1S3CAW6	2.4e-119	96.41	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 G...	[more]
A0A6J1KEU1	4.2e-119	96.41	Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=36...	[more]
A0A6J1G4U3	4.2e-119	96.41	Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=...	[more]

BLAST of HG10006810 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28230.1	8.1e-91	73.06	TATA-binding related factor (TRF) of subunit 20 of Mediator complex	[more]
AT4G09070.1	8.9e-82	64.98	TATA-binding related factor (TRF) of subunit 20 of Mediator complex	[more]
AT2G28020.1	3.8e-16	61.76	BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of...	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013921	Mediator complex, subunit Med20	PFAM	PF08612	Med20	coord: 1..208 e-value: 4.8E-43 score: 147.1
IPR013921	Mediator complex, subunit Med20	PANTHER	PTHR12465	UBIQUITIN SPECIFIC PROTEASE HOMOLOG 49	coord: 6..217
None	No IPR available	PANTHER	PTHR12465:SF0	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 20	coord: 6..217

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10006810.1	HG10006810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
biological_process	GO:0006383	transcription by RNA polymerase III
cellular_component	GO:0016592	mediator complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0003713	transcription coactivator activity
molecular_function	GO:0003712	transcription coregulator activity