HG10006461 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10006461 vs. NCBI nr
Match: XP_038877762.1 (GDSL esterase/lipase At1g54790-like [Benincasa hispida])

HSP 1 Score: 776.9 bits (2005), Expect = 7.8e-221
Identity = 368/379 (97.10%), Postives = 375/379 (98.94%), Query Frame = 0

Query: 1   MPALTHILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGH 60
           MPALT ILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIE+INPPYGRRFFGH
Sbjct: 1   MPALTQILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIETINPPYGRRFFGH 60

Query: 61  PSGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF 120
           PSGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF
Sbjct: 61  PSGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF 120

Query: 121 SFGIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT 180
           SFG+QVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT
Sbjct: 121 SFGVQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT 180

Query: 181 LDQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGC 240
           LDQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCL QNVAKFGTDPSK+DEFGC
Sbjct: 181 LDQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLAQNVAKFGTDPSKIDEFGC 240

Query: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQPIMACC 300
           VSSHNQAAKLFNLQLHALCKKLQGQYTDGN+TYIDI+SIKSNLIANYSRLGFEQPIMACC
Sbjct: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDGNITYIDIYSIKSNLIANYSRLGFEQPIMACC 300

Query: 301 GYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVSSQILSG 360
           GYGGPPLNYDSRI CGQTKTLNGTLVTAKGC+DSSEYINWDGIHYTEAANQYVSSQIL+G
Sbjct: 301 GYGGPPLNYDSRIVCGQTKTLNGTLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTG 360

Query: 361 KYCDPPFSDKMPFLLKLKF 380
           KY DPPFSDKMPFLLKLKF
Sbjct: 361 KYSDPPFSDKMPFLLKLKF 379

BLAST of HG10006461 vs. NCBI nr
Match: XP_008455063.1 (PREDICTED: GDSL esterase/lipase At1g54790-like isoform X2 [Cucumis melo])

HSP 1 Score: 755.4 bits (1949), Expect = 2.4e-214
Identity = 356/379 (93.93%), Postives = 366/379 (96.57%), Query Frame = 0

Query: 1   MPALTHILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGH 60
           M  LTHILTFFLLC   ARSNPFS PAVFNFGDSNSDTGCLVGAAIESINPPYG RFFGH
Sbjct: 1   MSTLTHILTFFLLCFSFARSNPFSRPAVFNFGDSNSDTGCLVGAAIESINPPYGHRFFGH 60

Query: 61  PSGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF 120
           PSGRYCDGRL+VDFLLDAMDMP+LN YLDS+GAPNFRKGCNYAAAGSTVLPATATSVSPF
Sbjct: 61  PSGRYCDGRLVVDFLLDAMDMPFLNAYLDSIGAPNFRKGCNYAAAGSTVLPATATSVSPF 120

Query: 121 SFGIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT 180
           SFG+QVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT
Sbjct: 121 SFGVQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT 180

Query: 181 LDQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGC 240
           LDQILASIPTILAEFESGVQ+LYDQGARNFWIHNTGPLGCL QNVAKFGTDPSKLDEFGC
Sbjct: 181 LDQILASIPTILAEFESGVQRLYDQGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC 240

Query: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQPIMACC 300
           VSSHNQAAKLFNLQLHALCKKLQGQYTDGN+TYIDI+SIKSNLIANYSRLGF+QPIM CC
Sbjct: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDGNITYIDIYSIKSNLIANYSRLGFQQPIMVCC 300

Query: 301 GYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVSSQILSG 360
           GYGGPPLNYDSRI CGQTK LNGTLVTAKGC+DSSEYINWDGIHYTEAANQYVSSQIL+G
Sbjct: 301 GYGGPPLNYDSRIVCGQTKMLNGTLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTG 360

Query: 361 KYCDPPFSDKMPFLLKLKF 380
           KY DPPFSDKMPFLLKLKF
Sbjct: 361 KYSDPPFSDKMPFLLKLKF 379

BLAST of HG10006461 vs. NCBI nr
Match: XP_004139194.1 (GDSL esterase/lipase At1g54790 [Cucumis sativus] >KGN60873.1 hypothetical protein Csa_023468 [Cucumis sativus])

HSP 1 Score: 750.7 bits (1937), Expect = 6.0e-213
Identity = 355/379 (93.67%), Postives = 366/379 (96.57%), Query Frame = 0

Query: 1   MPALTHILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGH 60
           M  LT ILTFFLLC P ARSNPFS PA+FNFGDSNSDTGCLVGAAIESINPPYG RFFGH
Sbjct: 1   MSTLTLILTFFLLCFPFARSNPFSRPAIFNFGDSNSDTGCLVGAAIESINPPYGHRFFGH 60

Query: 61  PSGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF 120
           PSGRYCDGRLIVDFLLDAMDMP+LN YLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF
Sbjct: 61  PSGRYCDGRLIVDFLLDAMDMPFLNAYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF 120

Query: 121 SFGIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT 180
           SFG+QVNQFLHFKARVLELREGKGGKKLDKYLPAE+YFQKGLYMFDIGQNDLAGAFYSKT
Sbjct: 121 SFGVQVNQFLHFKARVLELREGKGGKKLDKYLPAEEYFQKGLYMFDIGQNDLAGAFYSKT 180

Query: 181 LDQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGC 240
           LDQILASIPTILAEFESGVQKL+DQGARNFWIHNTGPLGCL QNVAKFGTDPSKLDEFGC
Sbjct: 181 LDQILASIPTILAEFESGVQKLFDQGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC 240

Query: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQPIMACC 300
           VSSHNQAAKLFNLQLHALCKKLQGQYTD N+TYIDI+SIKSNLIANYSRLGF+QPIMACC
Sbjct: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDSNITYIDIYSIKSNLIANYSRLGFQQPIMACC 300

Query: 301 GYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVSSQILSG 360
           GYGGPPLNYDSRI CGQTK LNGTLVTAKGC+DSSEYINWDGIHY+EAANQYVSSQIL+G
Sbjct: 301 GYGGPPLNYDSRIVCGQTKMLNGTLVTAKGCDDSSEYINWDGIHYSEAANQYVSSQILTG 360

Query: 361 KYCDPPFSDKMPFLLKLKF 380
           KY DPPFSDKMPFLLKLKF
Sbjct: 361 KYSDPPFSDKMPFLLKLKF 379

BLAST of HG10006461 vs. NCBI nr
Match: KAG6586279.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 727.6 bits (1877), Expect = 5.4e-206
Identity = 343/379 (90.50%), Postives = 358/379 (94.46%), Query Frame = 0

Query: 1   MPALTHILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGH 60
           M ALTH+L   LLC+  A SNPFSSPAVFNFGDSNSDTGC+VGAAIE+I PPYG RFFGH
Sbjct: 14  MAALTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGH 73

Query: 61  PSGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF 120
           PSGRY DGRLIVDFLLDAMDMPYLN YLDSLGAPNFRKGCNYAAAGST+LPATATS  PF
Sbjct: 74  PSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPF 133

Query: 121 SFGIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT 180
           SFG+QVNQFLHFKA+VLELR GKGGKKLDKYLPA+DYFQKGLYMFDIGQNDLAGAFYSKT
Sbjct: 134 SFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKT 193

Query: 181 LDQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGC 240
           LDQILASIP IL+EFESGVQKLYD+GARNFWIHNTGPLGCL QNVAKFGTDPSKLDEFGC
Sbjct: 194 LDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC 253

Query: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQPIMACC 300
           VSSHNQAAKLFNLQLHALCKKLQGQY DGNVTY+DI+SIKSNLIANYSRLGFEQPIMACC
Sbjct: 254 VSSHNQAAKLFNLQLHALCKKLQGQYADGNVTYVDIYSIKSNLIANYSRLGFEQPIMACC 313

Query: 301 GYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVSSQILSG 360
           GYGGPPLNYDSRI CG TK LNGTLVTAKGC+DSSEYINWDGIHYTEAANQYVSSQIL+G
Sbjct: 314 GYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTG 373

Query: 361 KYCDPPFSDKMPFLLKLKF 380
           KY DPPFS+KMPFLLKLKF
Sbjct: 374 KYSDPPFSEKMPFLLKLKF 392

BLAST of HG10006461 vs. NCBI nr
Match: XP_022937633.1 (GDSL esterase/lipase At1g54790-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 726.5 bits (1874), Expect = 1.2e-205
Identity = 342/379 (90.24%), Postives = 359/379 (94.72%), Query Frame = 0

Query: 1   MPALTHILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGH 60
           M ALTH+L   LLC+  A SNPFSSPAVFNFGDSNSDTGC+VGAAIE+I PPYG RFFGH
Sbjct: 14  MAALTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGH 73

Query: 61  PSGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF 120
           PSGRY DGRLIVDFLLDAMDMPYLN YLDSLGAPNFRKGCNYAAAGST+LPATATS  PF
Sbjct: 74  PSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPF 133

Query: 121 SFGIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT 180
           SFG+QVNQFLHFKA+VLELR GKGGKKLDKYLPA+DYFQKGLYMFDIGQNDLAGAFYSKT
Sbjct: 134 SFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKT 193

Query: 181 LDQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGC 240
           LDQILASIP IL+EFESGVQKLYD+GARNFWIHNTGPLGCL QNVAKFGTDPSKLDEFGC
Sbjct: 194 LDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC 253

Query: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQPIMACC 300
           VSSHNQAAKLFNLQLHALCKKLQGQY+DGNVTY+DI+SIKSNLIANYS+LGFEQPIMACC
Sbjct: 254 VSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQLGFEQPIMACC 313

Query: 301 GYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVSSQILSG 360
           GYGGPPLNYDSRI CG TK LNGTLVTAKGC+DSSEYINWDGIHYTEAANQYVSSQIL+G
Sbjct: 314 GYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTG 373

Query: 361 KYCDPPFSDKMPFLLKLKF 380
           KY DPPFS+KMPFLLKLKF
Sbjct: 374 KYSDPPFSEKMPFLLKLKF 392

BLAST of HG10006461 vs. ExPASy Swiss-Prot
Match: Q3ECP6 (GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 7.4e-158
Identity = 265/402 (65.92%), Postives = 313/402 (77.86%), Query Frame = 0

Query: 4   LTHILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGHPSG 63
           L +++ FF+  L I+ S  F+ P+ FNFGDSNSDTG LV      ++ P G+  F   S 
Sbjct: 8   LFYVILFFISSLQISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQ 67

Query: 64  RYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPFSFG 123
           R+CDGRL++DFL+D MD+P+LNPYLDSLG PNF+KGCN+AAAGST+LPA  TSVSPFSF 
Sbjct: 68  RFCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFD 127

Query: 124 IQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKTLDQ 183
           +Q++QF+ FK+R +EL   K G+K +KYLP  DY+ KGLYM DIGQND+AGAFYSKTLDQ
Sbjct: 128 LQISQFIRFKSRAIELL-SKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQ 187

Query: 184 ILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGCVSS 243
           +LASIP+IL  FE+G+++LY++G RN WIHNTGPLGCL QN+AKFGTD +KLDEFGCVSS
Sbjct: 188 VLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSS 247

Query: 244 HNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSR-------------- 303
           HNQAAKLFNLQLHA+  K Q QY D NVTY+DIFSIKSNLIANYSR              
Sbjct: 248 HNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGKHFTKPLIDLNH 307

Query: 304 ------------LGFEQPIMACCGYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEY 363
                       LGFE+P+MACCG GG PLNYDSRI CGQTK L+G  VTAK CNDSSEY
Sbjct: 308 LENVGYNKILNVLGFEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEY 367

Query: 364 INWDGIHYTEAANQYVSSQILSGKYCDPPFSDKMPFLLKLKF 380
           INWDGIHYTEAAN++VSSQIL+GKY DPPFSD+MPF L LKF
Sbjct: 368 INWDGIHYTEAANEFVSSQILTGKYSDPPFSDQMPFFLTLKF 408

BLAST of HG10006461 vs. ExPASy Swiss-Prot
Match: Q9MAA1 (GDSL esterase/lipase At3g05180 OS=Arabidopsis thaliana OX=3702 GN=At3g05180 PE=2 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 3.6e-128
Identity = 223/376 (59.31%), Postives = 277/376 (73.67%), Query Frame = 0

Query: 1   MPALTHILTFFLLCLPIAR-----------SNPFSSPAVFNFGDSNSDTGCLVGAAIESI 60
           M  L H L   LL + I+            SN F  PAVFNFGDSNSDTG L        
Sbjct: 1   METLFHTLLRLLLFVAISHTLSPLAGSFRISNDF--PAVFNFGDSNSDTGELSSGLGFLP 60

Query: 61  NPPYGRRFFGHP-SGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGST 120
            P Y   FF  P SGR+C+GRLIVDFL++A+D PYL PYLDS+    +R+GCN+AAA ST
Sbjct: 61  QPSYEITFFRSPTSGRFCNGRLIVDFLMEAIDRPYLRPYLDSISRQTYRRGCNFAAAAST 120

Query: 121 VLPATATSVSPFSFGIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIG 180
           +  A A S SPF FG+QV+QF+ FK++VL+L +    ++L +YLP+E +F  GLYMFDIG
Sbjct: 121 IQKANAASYSPFGFGVQVSQFITFKSKVLQLIQ--QDEELQRYLPSEYFFSNGLYMFDIG 180

Query: 181 QNDLAGAFYSKTLDQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKF 240
           QND+AGAFY+KT+DQ+LA +P IL  F+ G+++LY +GARN+WIHNTGPLGCL Q V+ F
Sbjct: 181 QNDIAGAFYTKTVDQVLALVPIILDIFQDGIKRLYAEGARNYWIHNTGPLGCLAQVVSIF 240

Query: 241 GTDPSKLDEFGCVSSHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYS 300
           G D SKLDEFGCVS HNQAAKLFNLQLH L KKL  QY +   TY+DIFSIKS+LI N+S
Sbjct: 241 GEDKSKLDEFGCVSDHNQAAKLFNLQLHGLFKKLPQQYPNSRFTYVDIFSIKSDLILNHS 300

Query: 301 RLGFEQPIMACCGYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEA 360
           + GF+  IM CCG GGPPLNYD ++GCG+T   NGT++TAK C DSS+Y+NWDGIHYTEA
Sbjct: 301 KYGFDHSIMVCCGTGGPPLNYDDQVGCGKTARSNGTIITAKPCYDSSKYVNWDGIHYTEA 360

Query: 361 ANQYVSSQILSGKYCD 365
           AN++V+  IL+GKY +
Sbjct: 361 ANRFVALHILTGKYSE 372

BLAST of HG10006461 vs. ExPASy Swiss-Prot
Match: O80522 (GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 4.0e-87
Identity = 169/372 (45.43%), Postives = 232/372 (62.37%), Query Frame = 0

Query: 6   HILTFFLLCLP---IARSNPFSS------PAVFNFGDSNSDTGCLVGAAIESINPPYGRR 65
           H  +F L+ LP   I R N   +      P +FNFGDSNSDTG LV     SI  P GR 
Sbjct: 7   HSHSFLLVLLPFILILRQNLAVAGGCQVPPVIFNFGDSNSDTGGLVAGLGYSIGLPNGRS 66

Query: 66  FFGHPSGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATS 125
           FF   +GR  DGRL++DFL  +++   LNPYLDSL    F+ G N+A  GS+ LP     
Sbjct: 67  FFQRSTGRLSDGRLVIDFLCQSLNTSLLNPYLDSLVGSKFQNGANFAIVGSSTLPRYV-- 126

Query: 126 VSPFSFGIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAF 185
             PF+  IQ+ QFLHFK+R LEL        L + +  E  F+  LYM DIGQND+A +F
Sbjct: 127 --PFALNIQLMQFLHFKSRALEL--ASISDPLKEMMIGESGFRNALYMIDIGQNDIADSF 186

Query: 186 YSKTL--DQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSK 245
            SK L   +++  IP +++E +S ++ LYD+G R FW+HNTGPLGCL Q ++        
Sbjct: 187 -SKGLSYSRVVKLIPNVISEIKSAIKILYDEGGRKFWVHNTGPLGCLPQKLSM--VHSKG 246

Query: 246 LDEFGCVSSHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQ 305
            D+ GC++++N AAKLFN  L  +C+ L+ +  + N+ Y+DI++IK +LIAN +  GFE+
Sbjct: 247 FDKHGCLATYNAAAKLFNEGLDHMCRDLRTELKEANIVYVDIYAIKYDLIANSNNYGFEK 306

Query: 306 PIMACCGYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVS 365
           P+MACCGYGGPP NY+  I CG     NG    +K C++ S +I+WDGIHYTE AN  V+
Sbjct: 307 PLMACCGYGGPPYNYNVNITCG-----NG---GSKSCDEGSRFISWDGIHYTETANAIVA 361

Query: 366 SQILSGKYCDPP 367
            ++LS ++  PP
Sbjct: 367 MKVLSMQHSTPP 361

BLAST of HG10006461 vs. ExPASy Swiss-Prot
Match: Q9FXB6 (GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 7.6e-86
Identity = 164/360 (45.56%), Postives = 218/360 (60.56%), Query Frame = 0

Query: 8   LTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGHPSGRYCD 67
           L   +L  P   ++  + P +FNFGDSNSDTG LV      I  P GR FF   +GR  D
Sbjct: 21  LALLILRQPSRAASCTARPVIFNFGDSNSDTGGLVAGLGYPIGFPNGRLFFRRSTGRLSD 80

Query: 68  GRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPFSFGIQVN 127
           GRL++DFL  +++   L PYLDSLG   F+ G N+A AGS  LP       PFS  IQV 
Sbjct: 81  GRLLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSPTLPKNV----PFSLNIQVK 140

Query: 128 QFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAF-YSKTLDQILA 187
           QF HFK+R LEL        L     + + F+  LYM DIGQND+A +F    +  Q + 
Sbjct: 141 QFSHFKSRSLEL--ASSSNSLKGMFISNNGFKNALYMIDIGQNDIARSFARGNSYSQTVK 200

Query: 188 SIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGCVSSHNQ 247
            IP I+ E +S +++LYD+G R FWIHNTGPLGCL Q ++        LD+ GC+ S+N 
Sbjct: 201 LIPQIITEIKSSIKRLYDEGGRRFWIHNTGPLGCLPQKLSM--VKSKDLDQHGCLVSYNS 260

Query: 248 AAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQPIMACCGYGGPP 307
           AA LFN  L  +C++L+ +  D  + YIDI++IK +LIAN ++ GF+ P+MACCGYGG P
Sbjct: 261 AATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSNQYGFKSPLMACCGYGGTP 320

Query: 308 LNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVSSQILSGKYCDPP 367
            NY+ +I CG      G+ V    C + S +I+WDGIHYTE AN  V+ ++LS  Y  PP
Sbjct: 321 YNYNVKITCGH----KGSNV----CEEGSRFISWDGIHYTETANAIVAMKVLSMHYSKPP 364

BLAST of HG10006461 vs. ExPASy Swiss-Prot
Match: Q9LIN2 (GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.5e-81
Identity = 163/370 (44.05%), Postives = 222/370 (60.00%), Query Frame = 0

Query: 7   ILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGHPSGRYC 66
           +L    L  P A S   + PA+FNFGDSNSDTG L  A+      P G+ FF  PSGR+ 
Sbjct: 11  VLLASCLIHPRACSPSCNFPAIFNFGDSNSDTGGL-SASFGQAPYPNGQTFFHSPSGRFS 70

Query: 67  DGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATAT----SVSPFSF 126
           DGRLI+DF+ + + +PYLN +LDS+G+ NF  G N+A AGSTV P  AT     VSP S 
Sbjct: 71  DGRLIIDFIAEELGLPYLNAFLDSIGS-NFSHGANFATAGSTVRPPNATIAQSGVSPISL 130

Query: 127 GIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDL-AGAFYSKTL 186
            +Q+ QF  F  R  +L   +GG    K LP ++YF + LY FDIGQNDL AG   + T 
Sbjct: 131 DVQLVQFSDFITR-SQLIRNRGG-VFKKLLPKKEYFSQALYTFDIGQNDLTAGLKLNMTS 190

Query: 187 DQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGCV 246
           DQI A IP +  +  + ++K+Y +G R FWIHNT PLGCL   + +F    S++D  GC 
Sbjct: 191 DQIKAYIPDVHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPASQIDNHGCA 250

Query: 247 SSHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQPIMACCG 306
              N+ A+ +N +L     +L+ + ++   TY+DI+SIK  LI    +LGF  P++ACCG
Sbjct: 251 IPRNEIARYYNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGFRYPLVACCG 310

Query: 307 YGGPPLNYDSRIGCGQTKTLNG-TLVTAKGCNDSSEYINWDGIHYTEAANQYVSSQILSG 366
           +GG   N++  I CG    + G  +V AK CND S  ++WDGIH+TE  N ++  QI  G
Sbjct: 311 HGG-KYNFNKLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSWIFQQINDG 370

Query: 367 KYCDPPFSDK 371
            + DPP   K
Sbjct: 371 AFSDPPLPVK 375

BLAST of HG10006461 vs. ExPASy TrEMBL
Match: A0A1S3C007 (GDSL esterase/lipase At1g54790-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495321 PE=3 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.2e-214
Identity = 356/379 (93.93%), Postives = 366/379 (96.57%), Query Frame = 0

Query: 1   MPALTHILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGH 60
           M  LTHILTFFLLC   ARSNPFS PAVFNFGDSNSDTGCLVGAAIESINPPYG RFFGH
Sbjct: 1   MSTLTHILTFFLLCFSFARSNPFSRPAVFNFGDSNSDTGCLVGAAIESINPPYGHRFFGH 60

Query: 61  PSGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF 120
           PSGRYCDGRL+VDFLLDAMDMP+LN YLDS+GAPNFRKGCNYAAAGSTVLPATATSVSPF
Sbjct: 61  PSGRYCDGRLVVDFLLDAMDMPFLNAYLDSIGAPNFRKGCNYAAAGSTVLPATATSVSPF 120

Query: 121 SFGIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT 180
           SFG+QVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT
Sbjct: 121 SFGVQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT 180

Query: 181 LDQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGC 240
           LDQILASIPTILAEFESGVQ+LYDQGARNFWIHNTGPLGCL QNVAKFGTDPSKLDEFGC
Sbjct: 181 LDQILASIPTILAEFESGVQRLYDQGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC 240

Query: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQPIMACC 300
           VSSHNQAAKLFNLQLHALCKKLQGQYTDGN+TYIDI+SIKSNLIANYSRLGF+QPIM CC
Sbjct: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDGNITYIDIYSIKSNLIANYSRLGFQQPIMVCC 300

Query: 301 GYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVSSQILSG 360
           GYGGPPLNYDSRI CGQTK LNGTLVTAKGC+DSSEYINWDGIHYTEAANQYVSSQIL+G
Sbjct: 301 GYGGPPLNYDSRIVCGQTKMLNGTLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTG 360

Query: 361 KYCDPPFSDKMPFLLKLKF 380
           KY DPPFSDKMPFLLKLKF
Sbjct: 361 KYSDPPFSDKMPFLLKLKF 379

BLAST of HG10006461 vs. ExPASy TrEMBL
Match: A0A0A0LGA7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G020960 PE=3 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 2.9e-213
Identity = 355/379 (93.67%), Postives = 366/379 (96.57%), Query Frame = 0

Query: 1   MPALTHILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGH 60
           M  LT ILTFFLLC P ARSNPFS PA+FNFGDSNSDTGCLVGAAIESINPPYG RFFGH
Sbjct: 1   MSTLTLILTFFLLCFPFARSNPFSRPAIFNFGDSNSDTGCLVGAAIESINPPYGHRFFGH 60

Query: 61  PSGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF 120
           PSGRYCDGRLIVDFLLDAMDMP+LN YLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF
Sbjct: 61  PSGRYCDGRLIVDFLLDAMDMPFLNAYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF 120

Query: 121 SFGIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT 180
           SFG+QVNQFLHFKARVLELREGKGGKKLDKYLPAE+YFQKGLYMFDIGQNDLAGAFYSKT
Sbjct: 121 SFGVQVNQFLHFKARVLELREGKGGKKLDKYLPAEEYFQKGLYMFDIGQNDLAGAFYSKT 180

Query: 181 LDQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGC 240
           LDQILASIPTILAEFESGVQKL+DQGARNFWIHNTGPLGCL QNVAKFGTDPSKLDEFGC
Sbjct: 181 LDQILASIPTILAEFESGVQKLFDQGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC 240

Query: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQPIMACC 300
           VSSHNQAAKLFNLQLHALCKKLQGQYTD N+TYIDI+SIKSNLIANYSRLGF+QPIMACC
Sbjct: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDSNITYIDIYSIKSNLIANYSRLGFQQPIMACC 300

Query: 301 GYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVSSQILSG 360
           GYGGPPLNYDSRI CGQTK LNGTLVTAKGC+DSSEYINWDGIHY+EAANQYVSSQIL+G
Sbjct: 301 GYGGPPLNYDSRIVCGQTKMLNGTLVTAKGCDDSSEYINWDGIHYSEAANQYVSSQILTG 360

Query: 361 KYCDPPFSDKMPFLLKLKF 380
           KY DPPFSDKMPFLLKLKF
Sbjct: 361 KYSDPPFSDKMPFLLKLKF 379

BLAST of HG10006461 vs. ExPASy TrEMBL
Match: A0A6J1FGI3 (GDSL esterase/lipase At1g54790-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443981 PE=3 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 5.8e-206
Identity = 342/379 (90.24%), Postives = 359/379 (94.72%), Query Frame = 0

Query: 1   MPALTHILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGH 60
           M ALTH+L   LLC+  A SNPFSSPAVFNFGDSNSDTGC+VGAAIE+I PPYG RFFGH
Sbjct: 14  MAALTHLLLLLLLCVSAATSNPFSSPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGH 73

Query: 61  PSGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF 120
           PSGRY DGRLIVDFLLDAMDMPYLN YLDSLGAPNFRKGCNYAAAGST+LPATATS  PF
Sbjct: 74  PSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPF 133

Query: 121 SFGIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT 180
           SFG+QVNQFLHFKA+VLELR GKGGKKLDKYLPA+DYFQKGLYMFDIGQNDLAGAFYSKT
Sbjct: 134 SFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKT 193

Query: 181 LDQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGC 240
           LDQILASIP IL+EFESGVQKLYD+GARNFWIHNTGPLGCL QNVAKFGTDPSKLDEFGC
Sbjct: 194 LDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC 253

Query: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQPIMACC 300
           VSSHNQAAKLFNLQLHALCKKLQGQY+DGNVTY+DI+SIKSNLIANYS+LGFEQPIMACC
Sbjct: 254 VSSHNQAAKLFNLQLHALCKKLQGQYSDGNVTYVDIYSIKSNLIANYSQLGFEQPIMACC 313

Query: 301 GYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVSSQILSG 360
           GYGGPPLNYDSRI CG TK LNGTLVTAKGC+DSSEYINWDGIHYTEAANQYVSSQIL+G
Sbjct: 314 GYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTG 373

Query: 361 KYCDPPFSDKMPFLLKLKF 380
           KY DPPFS+KMPFLLKLKF
Sbjct: 374 KYSDPPFSEKMPFLLKLKF 392

BLAST of HG10006461 vs. ExPASy TrEMBL
Match: A0A6J1HML2 (GDSL esterase/lipase At1g54790-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465565 PE=3 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 7.1e-204
Identity = 341/379 (89.97%), Postives = 356/379 (93.93%), Query Frame = 0

Query: 1   MPALTHILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGH 60
           M  LTH+L   LLC+  A SNPFS PAVFNFGDSNSDTGC+VGAAIE+I PPYG RFFGH
Sbjct: 1   MAPLTHLL--LLLCVSAATSNPFSRPAVFNFGDSNSDTGCVVGAAIENIYPPYGHRFFGH 60

Query: 61  PSGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPF 120
           PSGRY DGRLIVDFLLDAMDMPYLN YLDSLGAPNFRKGCNYAAAGST+LPATATS  PF
Sbjct: 61  PSGRYSDGRLIVDFLLDAMDMPYLNAYLDSLGAPNFRKGCNYAAAGSTILPATATSFCPF 120

Query: 121 SFGIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKT 180
           SFG+QVNQFLHFKA+VLELR GKGGKKLDKYLPA+DYFQKGLYMFDIGQNDLAGAFYSKT
Sbjct: 121 SFGVQVNQFLHFKAKVLELRAGKGGKKLDKYLPADDYFQKGLYMFDIGQNDLAGAFYSKT 180

Query: 181 LDQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGC 240
           LDQILASIP IL+EFESGVQKLYD+GARNFWIHNTGPLGCL QNVAKFGTDPSKLDEFGC
Sbjct: 181 LDQILASIPAILSEFESGVQKLYDEGARNFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGC 240

Query: 241 VSSHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQPIMACC 300
           VSSHNQAAKLFNLQLHALCKKLQGQY DGNVTY+DI+SIKSNLIANYSRLGFEQPIMACC
Sbjct: 241 VSSHNQAAKLFNLQLHALCKKLQGQYADGNVTYVDIYSIKSNLIANYSRLGFEQPIMACC 300

Query: 301 GYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVSSQILSG 360
           GYGGPPLNYDSRI CG TK LNGTLVTAKGC+DSSEYINWDGIHYTEAANQYVSSQIL+G
Sbjct: 301 GYGGPPLNYDSRIVCGHTKMLNGTLVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTG 360

Query: 361 KYCDPPFSDKMPFLLKLKF 380
           KY DPPFS+KMPFLLKLKF
Sbjct: 361 KYSDPPFSEKMPFLLKLKF 377

BLAST of HG10006461 vs. ExPASy TrEMBL
Match: A0A6J1DCM5 (GDSL esterase/lipase At1g54790-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018864 PE=3 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 2.3e-202
Identity = 338/377 (89.66%), Postives = 354/377 (93.90%), Query Frame = 0

Query: 3   ALTHILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGHPS 62
           A  HIL FFLLCLPI+RS  FS PAVFNFGDSNSDTGCLVGAA E+I PPYG RF G PS
Sbjct: 2   AAFHILAFFLLCLPISRSIHFSLPAVFNFGDSNSDTGCLVGAAFETIYPPYGHRFLGTPS 61

Query: 63  GRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPFSF 122
           GRYCDGRLIVDFLLDAMD+PYLN YLDS+G PNFRKGCN+AAAGSTVLPATATSVSPFSF
Sbjct: 62  GRYCDGRLIVDFLLDAMDLPYLNAYLDSIGVPNFRKGCNFAAAGSTVLPATATSVSPFSF 121

Query: 123 GIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKTLD 182
           G+QVNQFLHFKARVLELR  KG KKLDKYLPAED+FQKGLYMFDIGQNDLAGAFYSKTLD
Sbjct: 122 GVQVNQFLHFKARVLELRAAKGAKKLDKYLPAEDFFQKGLYMFDIGQNDLAGAFYSKTLD 181

Query: 183 QILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGCVS 242
           QILASIP+IL EFESGVQ+LYDQGAR FWIHNTGPLGCL QNVAKFGTDPSKLDEFGCVS
Sbjct: 182 QILASIPSILVEFESGVQRLYDQGARIFWIHNTGPLGCLAQNVAKFGTDPSKLDEFGCVS 241

Query: 243 SHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQPIMACCGY 302
           SHNQAAKLFNLQLHALCKKLQGQY DGN+TY+DI++IKSNLIANYSRLGFEQPIMACCG 
Sbjct: 242 SHNQAAKLFNLQLHALCKKLQGQYADGNITYVDIYTIKSNLIANYSRLGFEQPIMACCGN 301

Query: 303 GGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVSSQILSGKY 362
           GGPPLNYDSRI CGQTKTLNGT+VTAKGC+DSSEYINWDGIHYTEAANQYVSSQIL+GKY
Sbjct: 302 GGPPLNYDSRIVCGQTKTLNGTVVTAKGCDDSSEYINWDGIHYTEAANQYVSSQILTGKY 361

Query: 363 CDPPFSDKMPFLLKLKF 380
            DPPFSDKMPFLLKLKF
Sbjct: 362 SDPPFSDKMPFLLKLKF 378

BLAST of HG10006461 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 590.1 bits (1520), Expect = 1.3e-168
Identity = 275/375 (73.33%), Postives = 317/375 (84.53%), Query Frame = 0

Query: 7   ILTFFL--LCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGHPSGR 66
           I+ F L  L LP + S     PA+ NFGDSNSDTG L+ A IE++NPPYG+ +F  PSGR
Sbjct: 10  IVLFILISLFLPSSFSIILKYPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNLPSGR 69

Query: 67  YCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPFSFGI 126
           YCDGRLIVDFLLD MD+P+LNPYLDSLG PNF+KGCN+AAAGST+LPA  TSVSPFSF +
Sbjct: 70  YCDGRLIVDFLLDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDL 129

Query: 127 QVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKTLDQI 186
           Q++QF+ FK+R +EL   K G+K +KYLP  DY+ KGLYM DIGQND+AGAFYSKTLDQ+
Sbjct: 130 QISQFIRFKSRAIELL-SKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQV 189

Query: 187 LASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGCVSSH 246
           LASIP+IL  FE+G+++LY++G RN WIHNTGPLGCL QN+AKFGTD +KLDEFGCVSSH
Sbjct: 190 LASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSSH 249

Query: 247 NQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQPIMACCGYGG 306
           NQAAKLFNLQLHA+  K Q QY D NVTY+DIFSIKSNLIANYSR GFE+P+MACCG GG
Sbjct: 250 NQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCGVGG 309

Query: 307 PPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVSSQILSGKYCD 366
            PLNYDSRI CGQTK L+G  VTAK CNDSSEYINWDGIHYTEAAN++VSSQIL+GKY D
Sbjct: 310 APLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKYSD 369

Query: 367 PPFSDKMPFLLKLKF 380
           PPFSD+MPF L LKF
Sbjct: 370 PPFSDQMPFFLTLKF 383

BLAST of HG10006461 vs. TAIR 10
Match: AT1G54790.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 570.9 bits (1470), Expect = 7.9e-163
Identity = 264/376 (70.21%), Postives = 312/376 (82.98%), Query Frame = 0

Query: 4   LTHILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGHPSG 63
           L +++ FF+  L I+ S  F+ P+ FNFGDSNSDTG LV      ++ P G+  F   S 
Sbjct: 8   LFYVILFFISSLQISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQ 67

Query: 64  RYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPFSFG 123
           R+CDGRL++DFL+D MD+P+LNPYLDSLG PNF+KGCN+AAAGST+LPA  TSVSPFSF 
Sbjct: 68  RFCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFD 127

Query: 124 IQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKTLDQ 183
           +Q++QF+ FK+R +EL   K G+K +KYLP  DY+ KGLYM DIGQND+AGAFYSKTLDQ
Sbjct: 128 LQISQFIRFKSRAIELL-SKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQ 187

Query: 184 ILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGCVSS 243
           +LASIP+IL  FE+G+++LY++G RN WIHNTGPLGCL QN+AKFGTD +KLDEFGCVSS
Sbjct: 188 VLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSS 247

Query: 244 HNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQPIMACCGYG 303
           HNQAAKLFNLQLHA+  K Q QY D NVTY+DIFSIKSNLIANYSR GFE+P+MACCG G
Sbjct: 248 HNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCGVG 307

Query: 304 GPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVSSQILSGKYC 363
           G PLNYDSRI CGQTK L+G  VTAK CNDSSEYINWDGIHYTEAAN++VSSQIL+GKY 
Sbjct: 308 GAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKYS 367

Query: 364 DPPFSDKMPFLLKLKF 380
           DPPFSD+MPF L LKF
Sbjct: 368 DPPFSDQMPFFLTLKF 382

BLAST of HG10006461 vs. TAIR 10
Match: AT1G54790.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 558.1 bits (1437), Expect = 5.3e-159
Identity = 265/402 (65.92%), Postives = 313/402 (77.86%), Query Frame = 0

Query: 4   LTHILTFFLLCLPIARSNPFSSPAVFNFGDSNSDTGCLVGAAIESINPPYGRRFFGHPSG 63
           L +++ FF+  L I+ S  F+ P+ FNFGDSNSDTG LV      ++ P G+  F   S 
Sbjct: 8   LFYVILFFISSLQISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQ 67

Query: 64  RYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATSVSPFSFG 123
           R+CDGRL++DFL+D MD+P+LNPYLDSLG PNF+KGCN+AAAGST+LPA  TSVSPFSF 
Sbjct: 68  RFCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFD 127

Query: 124 IQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAFYSKTLDQ 183
           +Q++QF+ FK+R +EL   K G+K +KYLP  DY+ KGLYM DIGQND+AGAFYSKTLDQ
Sbjct: 128 LQISQFIRFKSRAIELL-SKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQ 187

Query: 184 ILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSKLDEFGCVSS 243
           +LASIP+IL  FE+G+++LY++G RN WIHNTGPLGCL QN+AKFGTD +KLDEFGCVSS
Sbjct: 188 VLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSS 247

Query: 244 HNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSR-------------- 303
           HNQAAKLFNLQLHA+  K Q QY D NVTY+DIFSIKSNLIANYSR              
Sbjct: 248 HNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGKHFTKPLIDLNH 307

Query: 304 ------------LGFEQPIMACCGYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEY 363
                       LGFE+P+MACCG GG PLNYDSRI CGQTK L+G  VTAK CNDSSEY
Sbjct: 308 LENVGYNKILNVLGFEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEY 367

Query: 364 INWDGIHYTEAANQYVSSQILSGKYCDPPFSDKMPFLLKLKF 380
           INWDGIHYTEAAN++VSSQIL+GKY DPPFSD+MPF L LKF
Sbjct: 368 INWDGIHYTEAANEFVSSQILTGKYSDPPFSDQMPFFLTLKF 408

BLAST of HG10006461 vs. TAIR 10
Match: AT3G05180.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 459.5 bits (1181), Expect = 2.6e-129
Identity = 223/376 (59.31%), Postives = 277/376 (73.67%), Query Frame = 0

Query: 1   MPALTHILTFFLLCLPIAR-----------SNPFSSPAVFNFGDSNSDTGCLVGAAIESI 60
           M  L H L   LL + I+            SN F  PAVFNFGDSNSDTG L        
Sbjct: 1   METLFHTLLRLLLFVAISHTLSPLAGSFRISNDF--PAVFNFGDSNSDTGELSSGLGFLP 60

Query: 61  NPPYGRRFFGHP-SGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGST 120
            P Y   FF  P SGR+C+GRLIVDFL++A+D PYL PYLDS+    +R+GCN+AAA ST
Sbjct: 61  QPSYEITFFRSPTSGRFCNGRLIVDFLMEAIDRPYLRPYLDSISRQTYRRGCNFAAAAST 120

Query: 121 VLPATATSVSPFSFGIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIG 180
           +  A A S SPF FG+QV+QF+ FK++VL+L +    ++L +YLP+E +F  GLYMFDIG
Sbjct: 121 IQKANAASYSPFGFGVQVSQFITFKSKVLQLIQ--QDEELQRYLPSEYFFSNGLYMFDIG 180

Query: 181 QNDLAGAFYSKTLDQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKF 240
           QND+AGAFY+KT+DQ+LA +P IL  F+ G+++LY +GARN+WIHNTGPLGCL Q V+ F
Sbjct: 181 QNDIAGAFYTKTVDQVLALVPIILDIFQDGIKRLYAEGARNYWIHNTGPLGCLAQVVSIF 240

Query: 241 GTDPSKLDEFGCVSSHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYS 300
           G D SKLDEFGCVS HNQAAKLFNLQLH L KKL  QY +   TY+DIFSIKS+LI N+S
Sbjct: 241 GEDKSKLDEFGCVSDHNQAAKLFNLQLHGLFKKLPQQYPNSRFTYVDIFSIKSDLILNHS 300

Query: 301 RLGFEQPIMACCGYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEA 360
           + GF+  IM CCG GGPPLNYD ++GCG+T   NGT++TAK C DSS+Y+NWDGIHYTEA
Sbjct: 301 KYGFDHSIMVCCGTGGPPLNYDDQVGCGKTARSNGTIITAKPCYDSSKYVNWDGIHYTEA 360

Query: 361 ANQYVSSQILSGKYCD 365
           AN++V+  IL+GKY +
Sbjct: 361 ANRFVALHILTGKYSE 372

BLAST of HG10006461 vs. TAIR 10
Match: AT1G09390.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 323.2 bits (827), Expect = 2.9e-88
Identity = 169/372 (45.43%), Postives = 232/372 (62.37%), Query Frame = 0

Query: 6   HILTFFLLCLP---IARSNPFSS------PAVFNFGDSNSDTGCLVGAAIESINPPYGRR 65
           H  +F L+ LP   I R N   +      P +FNFGDSNSDTG LV     SI  P GR 
Sbjct: 7   HSHSFLLVLLPFILILRQNLAVAGGCQVPPVIFNFGDSNSDTGGLVAGLGYSIGLPNGRS 66

Query: 66  FFGHPSGRYCDGRLIVDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAGSTVLPATATS 125
           FF   +GR  DGRL++DFL  +++   LNPYLDSL    F+ G N+A  GS+ LP     
Sbjct: 67  FFQRSTGRLSDGRLVIDFLCQSLNTSLLNPYLDSLVGSKFQNGANFAIVGSSTLPRYV-- 126

Query: 126 VSPFSFGIQVNQFLHFKARVLELREGKGGKKLDKYLPAEDYFQKGLYMFDIGQNDLAGAF 185
             PF+  IQ+ QFLHFK+R LEL        L + +  E  F+  LYM DIGQND+A +F
Sbjct: 127 --PFALNIQLMQFLHFKSRALEL--ASISDPLKEMMIGESGFRNALYMIDIGQNDIADSF 186

Query: 186 YSKTL--DQILASIPTILAEFESGVQKLYDQGARNFWIHNTGPLGCLGQNVAKFGTDPSK 245
            SK L   +++  IP +++E +S ++ LYD+G R FW+HNTGPLGCL Q ++        
Sbjct: 187 -SKGLSYSRVVKLIPNVISEIKSAIKILYDEGGRKFWVHNTGPLGCLPQKLSM--VHSKG 246

Query: 246 LDEFGCVSSHNQAAKLFNLQLHALCKKLQGQYTDGNVTYIDIFSIKSNLIANYSRLGFEQ 305
            D+ GC++++N AAKLFN  L  +C+ L+ +  + N+ Y+DI++IK +LIAN +  GFE+
Sbjct: 247 FDKHGCLATYNAAAKLFNEGLDHMCRDLRTELKEANIVYVDIYAIKYDLIANSNNYGFEK 306

Query: 306 PIMACCGYGGPPLNYDSRIGCGQTKTLNGTLVTAKGCNDSSEYINWDGIHYTEAANQYVS 365
           P+MACCGYGGPP NY+  I CG     NG    +K C++ S +I+WDGIHYTE AN  V+
Sbjct: 307 PLMACCGYGGPPYNYNVNITCG-----NG---GSKSCDEGSRFISWDGIHYTETANAIVA 361

Query: 366 SQILSGKYCDPP 367
            ++LS ++  PP
Sbjct: 367 MKVLSMQHSTPP 361