HG10005503 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10005503
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionAlpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
LocationChr07: 3093802 .. 3095112 (-)
RNA-Seq ExpressionHG10005503
SyntenyHG10005503
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTATTAATCAGAAACCTCGCCTCAAAGATGCTGCTCTTAGACGTTTTAAATTGGTTGTTATTGTGGCGTTATTGGGGCTACTGCTGTTTATGATTCTTATGGAAACAAATTCGTTTCAGAATTTTGCTGCTTGGTCTTCTGATGATATTGATGATGATCCATATCTTGGTTTTAGTCGTTTTGATTCTAATGATGTACTTAGGCTGCCCAAACAAAGTGAGTTGTCGATTCGTTTAGAAAAGCGAAATCGGTTGCCACCTCGAAACTTAGATCTATATCCAAGACTAGCCAAGGATCATATACCTATAGTACTTTATGTGCACAACCGACCGCAGTATCTCCGAGTGGTAGTCGATAGCCTGTCCAAAGTTATAGGAATAAGTGAAACCGTGCTAATTGTTAGCCATGATGGGTACTTTGAAGAAATGGACAAGCTTGTCCAGAGCATCAGGTTTTGCCAAGTGAAACAAATTTTTGCTCCTTACTCTCCGCATCTCTTTCCCAACAGGTTTCCCGGAGTCTCGCCTGCCGATTGTAAGGGCAAGGATGATCCAAAGACAATACATTGTAGAGGGAATCCTGATCAGTATGGAAACCATCGGTTGCCTAAAATTGTTTCGTTAAAGCACCATTGGTGGTGGATGATGAACACGGTGTGGGATGGATTGAAGGAGACGCGGGGGATGTCGGGTCATATTTTGTTTATTGAGGAAGATCATTTTATATTGCCAAATGCATATCGGAATCTGCAATTACTAGTGACATTGAAGCCTAAAAAATGTCCTGAATGCTATTCTGTGAATCTAGCTCCAAGTGATGTGAAGTCAAGAGGAGAGGGTAACAATTTTCTTGTTGCAGAGAGGATGGGTAATATCGGTTATGCTTTCAATTGGACTATTTGGAGGAAGATCCATAAACAAGCAAAGGAGTTCTGCTTTTTCGATGAATACAATTGGGATATAACGATGTGGGCAACCGTTTATCCCTCGTTTGGTAAGCCCGTTTATTCATTACGAGGGCCTTGGGCGAGTGCAGTTCATTTTGGCAAATGTGGTTTACACCAGGGTCAGGGGGACAGGGATGTTTGTATTGATAATGGTGTGTTAAACATTGACGTTAAAGATATCGACAAAATTGCCAACATTAATTCCGAATGGCGACTTGATGTCTTCAGAGATCAACCTGGCTATGGGGCTGGGTTCAAGGGTTGGGGAGGTTGGGGTGATGAAAGAGACCGTCAGTTGTGTCTAAATTTTGCTCACATGTATCATTCCGCACCAAACAGCCCAAACGTATCCCATTCCTGA

mRNA sequence

ATGGCTATTAATCAGAAACCTCGCCTCAAAGATGCTGCTCTTAGACGTTTTAAATTGGTTGTTATTGTGGCGTTATTGGGGCTACTGCTGTTTATGATTCTTATGGAAACAAATTCGTTTCAGAATTTTGCTGCTTGGTCTTCTGATGATATTGATGATGATCCATATCTTGGTTTTAGTCGTTTTGATTCTAATGATGTACTTAGGCTGCCCAAACAAAGTGAGTTGTCGATTCGTTTAGAAAAGCGAAATCGGTTGCCACCTCGAAACTTAGATCTATATCCAAGACTAGCCAAGGATCATATACCTATAGTACTTTATGTGCACAACCGACCGCAGTATCTCCGAGTGGTAGTCGATAGCCTGTCCAAAGTTATAGGAATAAGTGAAACCGTGCTAATTGTTAGCCATGATGGGTACTTTGAAGAAATGGACAAGCTTGTCCAGAGCATCAGGTTTTGCCAAGTGAAACAAATTTTTGCTCCTTACTCTCCGCATCTCTTTCCCAACAGGTTTCCCGGAGTCTCGCCTGCCGATTGTAAGGGCAAGGATGATCCAAAGACAATACATTGTAGAGGGAATCCTGATCAGTATGGAAACCATCGGTTGCCTAAAATTGTTTCGTTAAAGCACCATTGGTGGTGGATGATGAACACGGTGTGGGATGGATTGAAGGAGACGCGGGGGATGTCGGGTCATATTTTGTTTATTGAGGAAGATCATTTTATATTGCCAAATGCATATCGGAATCTGCAATTACTAGTGACATTGAAGCCTAAAAAATGTCCTGAATGCTATTCTGTGAATCTAGCTCCAAGTGATGTGAAGTCAAGAGGAGAGGGTAACAATTTTCTTGTTGCAGAGAGGATGGGTAATATCGGTTATGCTTTCAATTGGACTATTTGGAGGAAGATCCATAAACAAGCAAAGGAGTTCTGCTTTTTCGATGAATACAATTGGGATATAACGATGTGGGCAACCGTTTATCCCTCGTTTGGTAAGCCCGTTTATTCATTACGAGGGCCTTGGGCGAGTGCAGTTCATTTTGGCAAATGTGGTTTACACCAGGGTCAGGGGGACAGGGATGTTTGTATTGATAATGGTGTGTTAAACATTGACGTTAAAGATATCGACAAAATTGCCAACATTAATTCCGAATGGCGACTTGATGTCTTCAGAGATCAACCTGGCTATGGGGCTGGGTTCAAGGGTTGGGGAGGTTGGGGTGATGAAAGAGACCGTCAGTTGTGTCTAAATTTTGCTCACATGTATCATTCCGCACCAAACAGCCCAAACGTATCCCATTCCTGA

Coding sequence (CDS)

ATGGCTATTAATCAGAAACCTCGCCTCAAAGATGCTGCTCTTAGACGTTTTAAATTGGTTGTTATTGTGGCGTTATTGGGGCTACTGCTGTTTATGATTCTTATGGAAACAAATTCGTTTCAGAATTTTGCTGCTTGGTCTTCTGATGATATTGATGATGATCCATATCTTGGTTTTAGTCGTTTTGATTCTAATGATGTACTTAGGCTGCCCAAACAAAGTGAGTTGTCGATTCGTTTAGAAAAGCGAAATCGGTTGCCACCTCGAAACTTAGATCTATATCCAAGACTAGCCAAGGATCATATACCTATAGTACTTTATGTGCACAACCGACCGCAGTATCTCCGAGTGGTAGTCGATAGCCTGTCCAAAGTTATAGGAATAAGTGAAACCGTGCTAATTGTTAGCCATGATGGGTACTTTGAAGAAATGGACAAGCTTGTCCAGAGCATCAGGTTTTGCCAAGTGAAACAAATTTTTGCTCCTTACTCTCCGCATCTCTTTCCCAACAGGTTTCCCGGAGTCTCGCCTGCCGATTGTAAGGGCAAGGATGATCCAAAGACAATACATTGTAGAGGGAATCCTGATCAGTATGGAAACCATCGGTTGCCTAAAATTGTTTCGTTAAAGCACCATTGGTGGTGGATGATGAACACGGTGTGGGATGGATTGAAGGAGACGCGGGGGATGTCGGGTCATATTTTGTTTATTGAGGAAGATCATTTTATATTGCCAAATGCATATCGGAATCTGCAATTACTAGTGACATTGAAGCCTAAAAAATGTCCTGAATGCTATTCTGTGAATCTAGCTCCAAGTGATGTGAAGTCAAGAGGAGAGGGTAACAATTTTCTTGTTGCAGAGAGGATGGGTAATATCGGTTATGCTTTCAATTGGACTATTTGGAGGAAGATCCATAAACAAGCAAAGGAGTTCTGCTTTTTCGATGAATACAATTGGGATATAACGATGTGGGCAACCGTTTATCCCTCGTTTGGTAAGCCCGTTTATTCATTACGAGGGCCTTGGGCGAGTGCAGTTCATTTTGGCAAATGTGGTTTACACCAGGGTCAGGGGGACAGGGATGTTTGTATTGATAATGGTGTGTTAAACATTGACGTTAAAGATATCGACAAAATTGCCAACATTAATTCCGAATGGCGACTTGATGTCTTCAGAGATCAACCTGGCTATGGGGCTGGGTTCAAGGGTTGGGGAGGTTGGGGTGATGAAAGAGACCGTCAGTTGTGTCTAAATTTTGCTCACATGTATCATTCCGCACCAAACAGCCCAAACGTATCCCATTCCTGA

Protein sequence

MAINQKPRLKDAALRRFKLVVIVALLGLLLFMILMETNSFQNFAAWSSDDIDDDPYLGFSRFDSNDVLRLPKQSELSIRLEKRNRLPPRNLDLYPRLAKDHIPIVLYVHNRPQYLRVVVDSLSKVIGISETVLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNRFPGVSPADCKGKDDPKTIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFIEEDHFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNWTIWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGQGDRDVCIDNGVLNIDVKDIDKIANINSEWRLDVFRDQPGYGAGFKGWGGWGDERDRQLCLNFAHMYHSAPNSPNVSHS
Homology
BLAST of HG10005503 vs. NCBI nr
Match: XP_038888419.1 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Benincasa hispida])

HSP 1 Score: 873.2 bits (2255), Expect = 9.2e-250
Identity = 408/436 (93.58%), Postives = 422/436 (96.79%), Query Frame = 0

Query: 1   MAINQKPRLKDAALRRFKLVVIVALLGLLLFMILMETNSFQNFAAWSSDDIDDDPYLGFS 60
           MAINQKPR KDAALRRFKLVV VALLGLLLFMILMETNSF+NFAAWSSDDID+DPYLGF 
Sbjct: 1   MAINQKPRFKDAALRRFKLVVFVALLGLLLFMILMETNSFRNFAAWSSDDIDEDPYLGFD 60

Query: 61  RFDSNDVLRLPKQSELSIRLEKRNRLPPRNLDLYPRLAKDHIPIVLYVHNRPQYLRVVVD 120
           RFDSNDVLRLPKQSELSIRLEKRN LPP+NLDLYP+LAKDHIPIVLYVHNRPQYLRVVVD
Sbjct: 61  RFDSNDVLRLPKQSELSIRLEKRNWLPPQNLDLYPKLAKDHIPIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVIGISETVLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNRFPGVSPADC 180
           SLSKVIGISET+LIVSHDGYFEEMDKLV+SIRFCQVKQIFAPYSPHLFPN FPGVSP+DC
Sbjct: 121 SLSKVIGISETLLIVSHDGYFEEMDKLVRSIRFCQVKQIFAPYSPHLFPNSFPGVSPSDC 180

Query: 181 KGKDDPKTIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFIEED 240
           KGKDDPKTIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRG+SGHILFIEED
Sbjct: 181 KGKDDPKTIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGISGHILFIEED 240

Query: 241 HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNWT 300
           HFILPNAYRNLQLLVTL PKKCP CYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFN T
Sbjct: 241 HFILPNAYRNLQLLVTLNPKKCPHCYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNRT 300

Query: 301 IWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGQGD 360
           IWRKIHKQAKEFCFFD+YNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGQGD
Sbjct: 301 IWRKIHKQAKEFCFFDDYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGQGD 360

Query: 361 RDVCIDNGVLNIDVKDIDKIANINSEWRLDVFRDQPGYGAGFKGWGGWGDERDRQLCLNF 420
           RDVCIDNG LNIDV+DIDK+ANINSEW++DVFR QPGYGAGF+GWGGWGDERDRQLCLNF
Sbjct: 361 RDVCIDNGALNIDVRDIDKVANINSEWQVDVFRHQPGYGAGFRGWGGWGDERDRQLCLNF 420

Query: 421 AHMYHSAPNSPNVSHS 437
           AHMYHS  N  NVSHS
Sbjct: 421 AHMYHSTLNGTNVSHS 436

BLAST of HG10005503 vs. NCBI nr
Match: XP_008462069.1 (PREDICTED: alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucumis melo] >KAA0059203.1 alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucumis melo var. makuwa] >TYK19316.1 alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 872.8 bits (2254), Expect = 1.2e-249
Identity = 407/436 (93.35%), Postives = 421/436 (96.56%), Query Frame = 0

Query: 1   MAINQKPRLKDAALRRFKLVVIVALLGLLLFMILMETNSFQNFAAWSSDDIDDDPYLGFS 60
           MAINQKPRLKDAAL+RFKLVV V LLGLLLFMIL ETNS  NFAAWSSDDID+DPYLGF 
Sbjct: 1   MAINQKPRLKDAALKRFKLVVFVTLLGLLLFMILTETNSSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  RFDSNDVLRLPKQSELSIRLEKRNRLPPRNLDLYPRLAKDHIPIVLYVHNRPQYLRVVVD 120
            FDSN  LRLPKQSELSIRLEKRNRLPPRNLDLYP+LAK+HIPIVLYVHNRPQYLRVVVD
Sbjct: 61  HFDSNVELRLPKQSELSIRLEKRNRLPPRNLDLYPKLAKNHIPIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVIGISETVLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNRFPGVSPADC 180
           SL+KVIGISET+L+VSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPN FPGVSPADC
Sbjct: 121 SLAKVIGISETLLVVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180

Query: 181 KGKDDPKTIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFIEED 240
           KGKDDPK +HCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKET+GMSGHILFIEED
Sbjct: 181 KGKDDPKAVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEED 240

Query: 241 HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNWT 300
           HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEG+NFLVAERMGNIGYAFN T
Sbjct: 241 HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLVAERMGNIGYAFNRT 300

Query: 301 IWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGQGD 360
           +WRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRG WASAVHFGKCGLHQGQGD
Sbjct: 301 VWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGTWASAVHFGKCGLHQGQGD 360

Query: 361 RDVCIDNGVLNIDVKDIDKIANINSEWRLDVFRDQPGYGAGFKGWGGWGDERDRQLCLNF 420
           RDVCIDNGVLNIDVKDIDK+ANINSEWR+DVFRDQPGYGAGF+GWGGWGDERDRQLCLNF
Sbjct: 361 RDVCIDNGVLNIDVKDIDKVANINSEWRVDVFRDQPGYGAGFRGWGGWGDERDRQLCLNF 420

Query: 421 AHMYHSAPNSPNVSHS 437
           AHMYHS PN PNVSHS
Sbjct: 421 AHMYHSVPNGPNVSHS 436

BLAST of HG10005503 vs. NCBI nr
Match: XP_004144546.1 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucumis sativus] >KGN43409.1 hypothetical protein Csa_020290 [Cucumis sativus])

HSP 1 Score: 843.6 bits (2178), Expect = 7.8e-241
Identity = 394/436 (90.37%), Postives = 413/436 (94.72%), Query Frame = 0

Query: 1   MAINQKPRLKDAALRRFKLVVIVALLGLLLFMILMETNSFQNFAAWSSDDIDDDPYLGFS 60
           MAINQKPR KDAAL+RFKLVV V LLGLLLFMIL ETN   NFAAWSSDDID+DPYLGF 
Sbjct: 1   MAINQKPRPKDAALKRFKLVVFVTLLGLLLFMILTETNLSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  RFDSNDVLRLPKQSELSIRLEKRNRLPPRNLDLYPRLAKDHIPIVLYVHNRPQYLRVVVD 120
             DSN  LRLPKQS+LSIRLEKRNRLPPRNLDLYP+LA +HI IVLYVHNRPQYLRVVVD
Sbjct: 61  --DSNVELRLPKQSQLSIRLEKRNRLPPRNLDLYPKLANNHIAIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVIGISETVLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNRFPGVSPADC 180
           SL+KVIGISET+LIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPN FPGVSPADC
Sbjct: 121 SLAKVIGISETLLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180

Query: 181 KGKDDPKTIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFIEED 240
           KGKDDPK +HCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKET+GMSGHILFIEED
Sbjct: 181 KGKDDPKAVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEED 240

Query: 241 HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNWT 300
           HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEG+NFL+AERMGNIGYAFN T
Sbjct: 241 HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLIAERMGNIGYAFNRT 300

Query: 301 IWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGQGD 360
           +WRKI+KQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQG GD
Sbjct: 301 VWRKIYKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGHGD 360

Query: 361 RDVCIDNGVLNIDVKDIDKIANINSEWRLDVFRDQPGYGAGFKGWGGWGDERDRQLCLNF 420
            D+CIDNGVLNIDVKDIDK+ANINSEWR+D+FRDQPGYGAGF+GWGGWGDERDRQLCLNF
Sbjct: 361 NDICIDNGVLNIDVKDIDKVANINSEWRVDIFRDQPGYGAGFRGWGGWGDERDRQLCLNF 420

Query: 421 AHMYHSAPNSPNVSHS 437
           A MYHS  N PN+SHS
Sbjct: 421 ARMYHSMSNGPNISHS 434

BLAST of HG10005503 vs. NCBI nr
Match: XP_022969101.1 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucurbita maxima])

HSP 1 Score: 800.8 bits (2067), Expect = 5.8e-228
Identity = 370/430 (86.05%), Postives = 399/430 (92.79%), Query Frame = 0

Query: 1   MAINQKPRLKDAALRRFKLVVIVALLGLLLFMILMETNSFQNFAAWSSDDIDDDPYLGFS 60
           MAINQKPRLKD+ALRRFKLV++V LLG LLFMILMETN FQNF  W SD+I +DPY GF 
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60

Query: 61  RFDSNDVLRLPKQSELSIRLEKRNRLPPRNLDLYPRLAKDHIPIVLYVHNRPQYLRVVVD 120
           RFDSNDVL+LPKQ+ELS+RLEKRNRLP RNLDLYP LAKDHIPIVLYVHNRPQYLRVV+D
Sbjct: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120

Query: 121 SLSKVIGISETVLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNRFPGVSPADC 180
           SLSKV GISET+LIVSHDGYFEEMDKLVQ+IRFC VKQIFAPYSPHLFPN FPGVSP DC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180

Query: 181 KGKDDPK-TIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFIEE 240
           KGK  P  T +CRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGL+ETRG+SGHILFIEE
Sbjct: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNW 300
           DHFILPNAYRNLQLLVTLKPKKCPEC++VNLAPSDVKSRGEGNNFLVAERMGNIGYAFN 
Sbjct: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 TIWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGQG 360
           ++W+KIHK+AKEFCFFDEYNWDITMWATV+PSFG PVYSLRGPW SA+HFGKCGLHQGQ 
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 DRDVCIDNGVLNIDVKDIDKIANINSEWRLDVFRDQPGYGAGFKGWGGWGDERDRQLCLN 420
           + DVCIDNG+LNIDVKDID++ NINSEW ++VFR QPGYGAGF+GWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAHMYHSAPN 430
           FA MY S PN
Sbjct: 421 FAQMYRSMPN 430

BLAST of HG10005503 vs. NCBI nr
Match: KAG7011860.1 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 800.0 bits (2065), Expect = 9.9e-228
Identity = 371/430 (86.28%), Postives = 399/430 (92.79%), Query Frame = 0

Query: 1   MAINQKPRLKDAALRRFKLVVIVALLGLLLFMILMETNSFQNFAAWSSDDIDDDPYLGFS 60
           MAINQKPRLKD+ALRRFKLV++V LLG LLFMILMETN FQNFA WSSD+I +DPY GF 
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60

Query: 61  RFDSNDVLRLPKQSELSIRLEKRNRLPPRNLDLYPRLAKDHIPIVLYVHNRPQYLRVVVD 120
           RFDSND L+LPKQ+ELSIRLEKRNRLP RNLDLYP LAKDHIPI+LYVHNRPQYLRVV+D
Sbjct: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120

Query: 121 SLSKVIGISETVLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNRFPGVSPADC 180
           SLSKV GISET+LIVSHDGYFEEMDKLVQ+IRFC VKQIFAPYSPHLFP+ FPGVSP DC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180

Query: 181 KGKDDPK-TIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFIEE 240
            GK  P  T +CRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGL+ETRG+SGHILFIEE
Sbjct: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNW 300
           DHFILPNAYRNLQLLV+LKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFN 
Sbjct: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 TIWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGQG 360
           ++W+KIHK+AKEFCFFDEYNWDITMWATV+PSFG PVYSLRGPW SA+HFGKCGLHQGQ 
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 DRDVCIDNGVLNIDVKDIDKIANINSEWRLDVFRDQPGYGAGFKGWGGWGDERDRQLCLN 420
           + DVCIDNG+LNIDVKDIDK+ NINSEW ++VFR QPGYGAGF+GWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAHMYHSAPN 430
           FA MY S PN
Sbjct: 421 FAQMYRSTPN 430

BLAST of HG10005503 vs. ExPASy Swiss-Prot
Match: Q9FT88 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Arabidopsis thaliana OX=3702 GN=GNT2 PE=1 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 4.8e-161
Identity = 266/433 (61.43%), Postives = 326/433 (75.29%), Query Frame = 0

Query: 5   QKPRLKDAALRRFKLVVIVALLGLLLFMILMETNSFQNFAAWSSDDIDDDPYLGFSRFDS 64
           +K RL+D  L R  ++  V L  +L+ + +  T       A +   IDDD        D 
Sbjct: 6   KKQRLRDTGLCRLGILFAVTLSIVLMLVSVPRT-------ALNGSSIDDD----LDGLDK 65

Query: 65  N-------DVLRLPKQSELSIRLEKRNRLPPRNLDLYPRLAKDHIPIVLYVHNRPQYLRV 124
           +        +L + + + +S+RL +RN   PRN DL+P LAKD + IVLYVHNR QY RV
Sbjct: 66  DLEAKLNASLLSVARGNRMSLRLHRRNHFSPRNTDLFPDLAKDRVVIVLYVHNRAQYFRV 125

Query: 125 VVDSLSKVIGISETVLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNRFPGVSP 184
            V+SLSKV GISET+LIVSHDGYFEEM+++V+SI+FCQVKQIF+PYSPH++   FPGV+ 
Sbjct: 126 TVESLSKVKGISETLLIVSHDGYFEEMNRIVESIKFCQVKQIFSPYSPHIYRTSFPGVTL 185

Query: 185 ADCKGKDDPKTIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFI 244
            DCK K D    HC GNPDQYGNHR PKIVSLKHHWWWMMNTVWDGL+ET+G  GHILFI
Sbjct: 186 NDCKNKGDEAKGHCEGNPDQYGNHRSPKIVSLKHHWWWMMNTVWDGLEETKGHEGHILFI 245

Query: 245 EEDHFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAF 304
           EEDHF+ PNAYRN+Q L  LKP KCP+C++ NLAPSDVKSRGEG   LVAERMGN+GY+F
Sbjct: 246 EEDHFLFPNAYRNIQTLTRLKPAKCPDCFAANLAPSDVKSRGEGLESLVAERMGNVGYSF 305

Query: 305 NWTIWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQG 364
           N ++W  IH++A+EFCFFD+YNWDITMWATV+PSFG PVY+LRGP  SAVHFGKCGLHQG
Sbjct: 306 NRSVWENIHQKAREFCFFDDYNWDITMWATVFPSFGSPVYTLRGPRTSAVHFGKCGLHQG 365

Query: 365 QGDRDVCIDNGVLNIDVKDIDKIANINSEWRLDVFRDQPGYGAGFKGWGGWGDERDRQLC 424
           +GD   CIDNGV+NI+VK+ DK+ NI   W + V++ Q GY AGF+GWGGWGD+RDR LC
Sbjct: 366 RGDEGDCIDNGVVNIEVKETDKVVNIKEGWGVRVYKHQAGYKAGFEGWGGWGDDRDRHLC 425

Query: 425 LNFAHMYHSAPNS 431
           L+FA MY  + +S
Sbjct: 426 LDFATMYRYSSSS 427

BLAST of HG10005503 vs. ExPASy Swiss-Prot
Match: Q10469 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Homo sapiens OX=9606 GN=MGAT2 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.5e-48
Identity = 116/360 (32.22%), Postives = 184/360 (51.11%), Query Frame = 0

Query: 89  RNLDLYPRLAKDHIPIVLYVHNRPQYLRVVVDSLSKVIGISETVLIVSHDGYFEEMDKLV 148
           RN+D     A   + +V+ VHNRP+YLR+++DSL K  GI   ++I SHD +  E+++L+
Sbjct: 105 RNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVLVIFSHDFWSTEINQLI 164

Query: 149 QSIRFCQVKQIFAPYSPHLFPNRFPGVSPADCKGKDDPKTIHCR------GNPDQYGNHR 208
             + FC V Q+F P+S  L+PN FPG  P DC  +D PK    +        PD +G++R
Sbjct: 165 AGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCP-RDLPKNAALKLGCINAEYPDSFGHYR 224

Query: 209 LPKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFIEEDHFILPNAYRNLQLLVTLKPKKC 268
             K    KHHWWW ++ VW+ +K  R  +G ILF+EEDH++ P+ Y   + +  LK ++C
Sbjct: 225 EAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQQEC 284

Query: 269 PEC-------YSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNWTIWRKIHKQAKEFCFF 328
           PEC       YS + +   +  + +   +   E   N+G A     ++K+ +    FC +
Sbjct: 285 PECDVLSLGTYSASRSFYGMADKVDVKTWKSTEH--NMGLALTRNAYQKLIECTDTFCTY 344

Query: 329 DEYNWDITM-WATV--YPSFGKPVYSLRGPWASAV-HFGKCGLHQGQGDRDVCIDNGVLN 388
           D+YNWD T+ + TV   P F K +     P    + H G CG+H  +  R         +
Sbjct: 345 DDYNWDWTLQYLTVSCLPKFWKVLV----PQIPRIFHAGDCGMHHKKTCRP--------S 404

Query: 389 IDVKDIDKIANINSEWRLDVFRDQPGYGAGF--------KGWGGWGDERDRQLCLNFAHM 424
                I+ + N N ++   +F +       F        +  GGWGD RD +LC ++  +
Sbjct: 405 TQSAQIESLLNNNKQY---MFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCKSYRRL 446

BLAST of HG10005503 vs. ExPASy Swiss-Prot
Match: O19071 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Sus scrofa OX=9823 GN=MGAT2 PE=3 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 2.1e-47
Identity = 112/355 (31.55%), Postives = 180/355 (50.70%), Query Frame = 0

Query: 89  RNLDLYPRLAKDHIPIVLYVHNRPQYLRVVVDSLSKVIGISETVLIVSHDGYFEEMDKLV 148
           RN+D         + +V+ VHNR +YL++++DSL K  GI   ++I SHD +  E+++L+
Sbjct: 105 RNVDKVSSWVPRELVLVVQVHNRAEYLKLLLDSLRKAQGIDNVLVIFSHDFWSTEINQLI 164

Query: 149 QSIRFCQVKQIFAPYSPHLFPNRFPGVSPADCK---GKDDPKTIHC--RGNPDQYGNHRL 208
             + FC V Q+F P+S  L+PN FPG  P DC     K+    + C     PD +G++R 
Sbjct: 165 AGVDFCPVLQVFFPFSIQLYPNEFPGTDPRDCPRDLEKNAALKMGCINAEYPDSFGHYRE 224

Query: 209 PKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFIEEDHFILPNAYRNLQLLVTLKPKKCP 268
            K    KHHWWW ++ VW+ +K  R  +G ILF+EEDH++ P+ Y   + +  LK ++CP
Sbjct: 225 AKFSQTKHHWWWKLHFVWERVKVLRDYAGLILFLEEDHYVAPDFYHVFKKMWNLKQQECP 284

Query: 269 EC-------YSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNWTIWRKIHKQAKEFCFFD 328
           EC       Y+   +  DV  + +   +   E   N+G A     ++K+ +    FC +D
Sbjct: 285 ECDVLSLGTYTTVRSFRDVADKVDVKTWKSTEH--NMGLALTRDAYQKLIECTDTFCTYD 344

Query: 329 EYNWDITM-WATV--YPSFGKPVYSLRGPWASAV-HFGKCGLHQGQGDRDVCIDNGVLNI 388
           +YNWD T+ + TV   P F K +     P    + H G CG+H  +  R         + 
Sbjct: 345 DYNWDWTLQYLTVSCLPKFWKVLV----PQVPRIFHAGDCGMHHKKTCRP--------ST 404

Query: 389 DVKDIDKIANINSEW----RLDVFRDQPGYGAGFKGWGGWGDERDRQLCLNFAHM 424
               I+ + N N ++     L +        +  +  GGWGD RD +LC ++  +
Sbjct: 405 QSAQIESLLNSNKQYMFPETLTISEKLTAALSPPRKNGGWGDIRDHELCKSYRRL 445

BLAST of HG10005503 vs. ExPASy Swiss-Prot
Match: Q09326 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Rattus norvegicus OX=10116 GN=Mgat2 PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 2.1e-47
Identity = 112/358 (31.28%), Postives = 182/358 (50.84%), Query Frame = 0

Query: 89  RNLDLYPRLAKDHIPIVLYVHNRPQYLRVVVDSLSKVIGISETVLIVSHDGYFEEMDKLV 148
           RN+D     +   + +V+ VHNRP+YLR+++DSL K  GI E ++I SHD +  E++ L+
Sbjct: 100 RNVDKDGTWSPGELVLVVQVHNRPEYLRLLIDSLRKAQGIREVLVIFSHDFWSAEINSLI 159

Query: 149 QSIRFCQVKQIFAPYSPHLFPNRFPGVSPADCK---GKDDPKTIHC--RGNPDQYGNHRL 208
            S+ FC V Q+F P+S  L+P+ FPG  P DC     K+    + C     PD +G++R 
Sbjct: 160 SSVDFCPVLQVFFPFSIQLYPSEFPGSDPRDCPRDLKKNAALKLGCINAEYPDSFGHYRE 219

Query: 209 PKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFIEEDHFILPNAYRNLQLLVTLKPKKCP 268
            K    KHHWWW ++ VW+ +K  +  +G ILF+EEDH++ P+ Y   + +  LK ++CP
Sbjct: 220 AKFSQTKHHWWWKLHFVWERVKVLQDYTGLILFLEEDHYLAPDFYHVFKKMWKLKQQECP 279

Query: 269 ECYSVNLAPSDVKSRGEGNNFLVAERMG---------NIGYAFNWTIWRKIHKQAKEFCF 328
            C  ++L          G    +A+++          N+G A     ++K+ +    FC 
Sbjct: 280 GCDVLSLGTYTTIRSFYG----IADKVDVKTWKSTEHNMGLALTRDAYQKLIECTDTFCT 339

Query: 329 FDEYNWDITMWATVYPSFGKPVYSLRGPWASAV-HFGKCGLHQGQGDRDVCIDNGVLNID 388
           +D+YNWD T+         K V+ +  P A  + H G CG+H  +  R         +  
Sbjct: 340 YDDYNWDWTLQYLTLACLPK-VWKVLVPQAPRIFHAGDCGMHHKKTCRP--------STQ 399

Query: 389 VKDIDKIANINSEWRLDVFRDQPGYGAGF--------KGWGGWGDERDRQLCLNFAHM 424
              I+ + N N ++   +F +    G  F        +  GGWGD RD +LC ++  +
Sbjct: 400 SAQIESLLNNNKQY---LFPETLVIGEKFPMAAISPPRKNGGWGDIRDHELCKSYRRL 441

BLAST of HG10005503 vs. ExPASy Swiss-Prot
Match: Q921V5 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Mus musculus OX=10090 GN=Mgat2 PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 8.0e-47
Identity = 108/343 (31.49%), Postives = 175/343 (51.02%), Query Frame = 0

Query: 104 IVLYVHNRPQYLRVVVDSLSKVIGISETVLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPY 163
           +V+ VHNRP+YLR+++DSL K  GI E ++I SHD +  E++ L+  + FC V Q+F P+
Sbjct: 115 LVVQVHNRPEYLRLLIDSLRKAQGIQEVLVIFSHDFWSAEINSLISRVDFCPVLQVFFPF 174

Query: 164 SPHLFPNRFPGVSPADCK---GKDDPKTIHC--RGNPDQYGNHRLPKIVSLKHHWWWMMN 223
           S  L+PN FPG  P DC     K+    + C     PD +G++R  K    KHHWWW ++
Sbjct: 175 SIQLYPNEFPGSDPRDCPRDLKKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLH 234

Query: 224 TVWDGLKETRGMSGHILFIEEDHFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSR 283
            VW+ +K  +  +G ILF+EEDH++ P+ Y   + +  LK ++CP C  ++L        
Sbjct: 235 FVWERVKVLQDYTGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPGCDVLSLGTYTTIRS 294

Query: 284 GEGNNFLVAERMG---------NIGYAFNWTIWRKIHKQAKEFCFFDEYNWDITMWATVY 343
             G    +A+++          N+G A     ++K+ +    FC +D+YNWD T+     
Sbjct: 295 FYG----IADKVDVKTWKSTEHNMGLALTRDAYQKLIECTDTFCTYDDYNWDWTLQYLTL 354

Query: 344 PSFGKPVYSLRGPWASAV-HFGKCGLHQGQGDRDVCIDNGVLNIDVKDIDKIANINSEWR 403
               K ++ +  P A  + H G CG+H  +  R         +     I+ + N N ++ 
Sbjct: 355 ACLPK-IWKVLVPQAPRIFHAGDCGMHHKKTCRP--------STQSAQIESLLNSNKQY- 414

Query: 404 LDVFRDQPGYGAGF--------KGWGGWGDERDRQLCLNFAHM 424
             +F +    G  F        +  GGWGD RD +LC ++  +
Sbjct: 415 --LFPETLVIGEKFPMAAISPPRKNGGWGDIRDHELCKSYRRL 441

BLAST of HG10005503 vs. ExPASy TrEMBL
Match: A0A5A7UTF0 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold295G00360 PE=3 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 5.8e-250
Identity = 407/436 (93.35%), Postives = 421/436 (96.56%), Query Frame = 0

Query: 1   MAINQKPRLKDAALRRFKLVVIVALLGLLLFMILMETNSFQNFAAWSSDDIDDDPYLGFS 60
           MAINQKPRLKDAAL+RFKLVV V LLGLLLFMIL ETNS  NFAAWSSDDID+DPYLGF 
Sbjct: 1   MAINQKPRLKDAALKRFKLVVFVTLLGLLLFMILTETNSSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  RFDSNDVLRLPKQSELSIRLEKRNRLPPRNLDLYPRLAKDHIPIVLYVHNRPQYLRVVVD 120
            FDSN  LRLPKQSELSIRLEKRNRLPPRNLDLYP+LAK+HIPIVLYVHNRPQYLRVVVD
Sbjct: 61  HFDSNVELRLPKQSELSIRLEKRNRLPPRNLDLYPKLAKNHIPIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVIGISETVLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNRFPGVSPADC 180
           SL+KVIGISET+L+VSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPN FPGVSPADC
Sbjct: 121 SLAKVIGISETLLVVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180

Query: 181 KGKDDPKTIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFIEED 240
           KGKDDPK +HCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKET+GMSGHILFIEED
Sbjct: 181 KGKDDPKAVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEED 240

Query: 241 HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNWT 300
           HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEG+NFLVAERMGNIGYAFN T
Sbjct: 241 HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLVAERMGNIGYAFNRT 300

Query: 301 IWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGQGD 360
           +WRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRG WASAVHFGKCGLHQGQGD
Sbjct: 301 VWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGTWASAVHFGKCGLHQGQGD 360

Query: 361 RDVCIDNGVLNIDVKDIDKIANINSEWRLDVFRDQPGYGAGFKGWGGWGDERDRQLCLNF 420
           RDVCIDNGVLNIDVKDIDK+ANINSEWR+DVFRDQPGYGAGF+GWGGWGDERDRQLCLNF
Sbjct: 361 RDVCIDNGVLNIDVKDIDKVANINSEWRVDVFRDQPGYGAGFRGWGGWGDERDRQLCLNF 420

Query: 421 AHMYHSAPNSPNVSHS 437
           AHMYHS PN PNVSHS
Sbjct: 421 AHMYHSVPNGPNVSHS 436

BLAST of HG10005503 vs. ExPASy TrEMBL
Match: A0A1S3CG23 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumis melo OX=3656 GN=LOC103500510 PE=3 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 5.8e-250
Identity = 407/436 (93.35%), Postives = 421/436 (96.56%), Query Frame = 0

Query: 1   MAINQKPRLKDAALRRFKLVVIVALLGLLLFMILMETNSFQNFAAWSSDDIDDDPYLGFS 60
           MAINQKPRLKDAAL+RFKLVV V LLGLLLFMIL ETNS  NFAAWSSDDID+DPYLGF 
Sbjct: 1   MAINQKPRLKDAALKRFKLVVFVTLLGLLLFMILTETNSSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  RFDSNDVLRLPKQSELSIRLEKRNRLPPRNLDLYPRLAKDHIPIVLYVHNRPQYLRVVVD 120
            FDSN  LRLPKQSELSIRLEKRNRLPPRNLDLYP+LAK+HIPIVLYVHNRPQYLRVVVD
Sbjct: 61  HFDSNVELRLPKQSELSIRLEKRNRLPPRNLDLYPKLAKNHIPIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVIGISETVLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNRFPGVSPADC 180
           SL+KVIGISET+L+VSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPN FPGVSPADC
Sbjct: 121 SLAKVIGISETLLVVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180

Query: 181 KGKDDPKTIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFIEED 240
           KGKDDPK +HCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKET+GMSGHILFIEED
Sbjct: 181 KGKDDPKAVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEED 240

Query: 241 HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNWT 300
           HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEG+NFLVAERMGNIGYAFN T
Sbjct: 241 HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLVAERMGNIGYAFNRT 300

Query: 301 IWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGQGD 360
           +WRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRG WASAVHFGKCGLHQGQGD
Sbjct: 301 VWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGTWASAVHFGKCGLHQGQGD 360

Query: 361 RDVCIDNGVLNIDVKDIDKIANINSEWRLDVFRDQPGYGAGFKGWGGWGDERDRQLCLNF 420
           RDVCIDNGVLNIDVKDIDK+ANINSEWR+DVFRDQPGYGAGF+GWGGWGDERDRQLCLNF
Sbjct: 361 RDVCIDNGVLNIDVKDIDKVANINSEWRVDVFRDQPGYGAGFRGWGGWGDERDRQLCLNF 420

Query: 421 AHMYHSAPNSPNVSHS 437
           AHMYHS PN PNVSHS
Sbjct: 421 AHMYHSVPNGPNVSHS 436

BLAST of HG10005503 vs. ExPASy TrEMBL
Match: A0A0A0K343 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G031700 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 3.8e-241
Identity = 394/436 (90.37%), Postives = 413/436 (94.72%), Query Frame = 0

Query: 1   MAINQKPRLKDAALRRFKLVVIVALLGLLLFMILMETNSFQNFAAWSSDDIDDDPYLGFS 60
           MAINQKPR KDAAL+RFKLVV V LLGLLLFMIL ETN   NFAAWSSDDID+DPYLGF 
Sbjct: 1   MAINQKPRPKDAALKRFKLVVFVTLLGLLLFMILTETNLSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  RFDSNDVLRLPKQSELSIRLEKRNRLPPRNLDLYPRLAKDHIPIVLYVHNRPQYLRVVVD 120
             DSN  LRLPKQS+LSIRLEKRNRLPPRNLDLYP+LA +HI IVLYVHNRPQYLRVVVD
Sbjct: 61  --DSNVELRLPKQSQLSIRLEKRNRLPPRNLDLYPKLANNHIAIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVIGISETVLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNRFPGVSPADC 180
           SL+KVIGISET+LIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPN FPGVSPADC
Sbjct: 121 SLAKVIGISETLLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180

Query: 181 KGKDDPKTIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFIEED 240
           KGKDDPK +HCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKET+GMSGHILFIEED
Sbjct: 181 KGKDDPKAVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEED 240

Query: 241 HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNWT 300
           HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEG+NFL+AERMGNIGYAFN T
Sbjct: 241 HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLIAERMGNIGYAFNRT 300

Query: 301 IWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGQGD 360
           +WRKI+KQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQG GD
Sbjct: 301 VWRKIYKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGHGD 360

Query: 361 RDVCIDNGVLNIDVKDIDKIANINSEWRLDVFRDQPGYGAGFKGWGGWGDERDRQLCLNF 420
            D+CIDNGVLNIDVKDIDK+ANINSEWR+D+FRDQPGYGAGF+GWGGWGDERDRQLCLNF
Sbjct: 361 NDICIDNGVLNIDVKDIDKVANINSEWRVDIFRDQPGYGAGFRGWGGWGDERDRQLCLNF 420

Query: 421 AHMYHSAPNSPNVSHS 437
           A MYHS  N PN+SHS
Sbjct: 421 ARMYHSMSNGPNISHS 434

BLAST of HG10005503 vs. ExPASy TrEMBL
Match: A0A6J1HWS5 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468194 PE=3 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 2.8e-228
Identity = 370/430 (86.05%), Postives = 399/430 (92.79%), Query Frame = 0

Query: 1   MAINQKPRLKDAALRRFKLVVIVALLGLLLFMILMETNSFQNFAAWSSDDIDDDPYLGFS 60
           MAINQKPRLKD+ALRRFKLV++V LLG LLFMILMETN FQNF  W SD+I +DPY GF 
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60

Query: 61  RFDSNDVLRLPKQSELSIRLEKRNRLPPRNLDLYPRLAKDHIPIVLYVHNRPQYLRVVVD 120
           RFDSNDVL+LPKQ+ELS+RLEKRNRLP RNLDLYP LAKDHIPIVLYVHNRPQYLRVV+D
Sbjct: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120

Query: 121 SLSKVIGISETVLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNRFPGVSPADC 180
           SLSKV GISET+LIVSHDGYFEEMDKLVQ+IRFC VKQIFAPYSPHLFPN FPGVSP DC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180

Query: 181 KGKDDPK-TIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFIEE 240
           KGK  P  T +CRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGL+ETRG+SGHILFIEE
Sbjct: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNW 300
           DHFILPNAYRNLQLLVTLKPKKCPEC++VNLAPSDVKSRGEGNNFLVAERMGNIGYAFN 
Sbjct: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 TIWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGQG 360
           ++W+KIHK+AKEFCFFDEYNWDITMWATV+PSFG PVYSLRGPW SA+HFGKCGLHQGQ 
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 DRDVCIDNGVLNIDVKDIDKIANINSEWRLDVFRDQPGYGAGFKGWGGWGDERDRQLCLN 420
           + DVCIDNG+LNIDVKDID++ NINSEW ++VFR QPGYGAGF+GWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAHMYHSAPN 430
           FA MY S PN
Sbjct: 421 FAQMYRSMPN 430

BLAST of HG10005503 vs. ExPASy TrEMBL
Match: A0A6J1GLB7 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455025 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 3.1e-227
Identity = 370/430 (86.05%), Postives = 397/430 (92.33%), Query Frame = 0

Query: 1   MAINQKPRLKDAALRRFKLVVIVALLGLLLFMILMETNSFQNFAAWSSDDIDDDPYLGFS 60
           MAINQKPRLKD+ALRRFKLV++V LLG LLFMILMETN FQNFA WSSD+I +DPY GF 
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60

Query: 61  RFDSNDVLRLPKQSELSIRLEKRNRLPPRNLDLYPRLAKDHIPIVLYVHNRPQYLRVVVD 120
           RFDSND L+LPKQ+ELSIRLEKRNRLP RNLDLYP LAKDHIPIVLYVHNRPQYLRVV+D
Sbjct: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120

Query: 121 SLSKVIGISETVLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNRFPGVSPADC 180
           SLSKV GISET+LIVSHDGYFEEMDKLVQ+IRFC VKQIFAPYSPHLFP+ FPGVSP DC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180

Query: 181 KGKDDPK-TIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFIEE 240
            GK  P  T +CRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGL+ETRG+SGHILFIEE
Sbjct: 181 MGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNW 300
           DHFILPNAYRNLQ LV+LKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFN 
Sbjct: 241 DHFILPNAYRNLQFLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 TIWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGQG 360
           ++W+KIHK+AKEFCFFDEYNWDITMWATV+PSFG  VYSLRGPW SA+HFGKCGLHQGQ 
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSSVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 DRDVCIDNGVLNIDVKDIDKIANINSEWRLDVFRDQPGYGAGFKGWGGWGDERDRQLCLN 420
           + DVCIDNG+LNIDVKDIDK+ NINSEW ++VFR QPGYGAGF+GWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAHMYHSAPN 430
           FA MY S PN
Sbjct: 421 FAQMYRSTPN 430

BLAST of HG10005503 vs. TAIR 10
Match: AT2G05320.1 (beta-1,2-N-acetylglucosaminyltransferase II )

HSP 1 Score: 568.9 bits (1465), Expect = 3.4e-162
Identity = 266/433 (61.43%), Postives = 326/433 (75.29%), Query Frame = 0

Query: 5   QKPRLKDAALRRFKLVVIVALLGLLLFMILMETNSFQNFAAWSSDDIDDDPYLGFSRFDS 64
           +K RL+D  L R  ++  V L  +L+ + +  T       A +   IDDD        D 
Sbjct: 6   KKQRLRDTGLCRLGILFAVTLSIVLMLVSVPRT-------ALNGSSIDDD----LDGLDK 65

Query: 65  N-------DVLRLPKQSELSIRLEKRNRLPPRNLDLYPRLAKDHIPIVLYVHNRPQYLRV 124
           +        +L + + + +S+RL +RN   PRN DL+P LAKD + IVLYVHNR QY RV
Sbjct: 66  DLEAKLNASLLSVARGNRMSLRLHRRNHFSPRNTDLFPDLAKDRVVIVLYVHNRAQYFRV 125

Query: 125 VVDSLSKVIGISETVLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNRFPGVSP 184
            V+SLSKV GISET+LIVSHDGYFEEM+++V+SI+FCQVKQIF+PYSPH++   FPGV+ 
Sbjct: 126 TVESLSKVKGISETLLIVSHDGYFEEMNRIVESIKFCQVKQIFSPYSPHIYRTSFPGVTL 185

Query: 185 ADCKGKDDPKTIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGMSGHILFI 244
            DCK K D    HC GNPDQYGNHR PKIVSLKHHWWWMMNTVWDGL+ET+G  GHILFI
Sbjct: 186 NDCKNKGDEAKGHCEGNPDQYGNHRSPKIVSLKHHWWWMMNTVWDGLEETKGHEGHILFI 245

Query: 245 EEDHFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAF 304
           EEDHF+ PNAYRN+Q L  LKP KCP+C++ NLAPSDVKSRGEG   LVAERMGN+GY+F
Sbjct: 246 EEDHFLFPNAYRNIQTLTRLKPAKCPDCFAANLAPSDVKSRGEGLESLVAERMGNVGYSF 305

Query: 305 NWTIWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQG 364
           N ++W  IH++A+EFCFFD+YNWDITMWATV+PSFG PVY+LRGP  SAVHFGKCGLHQG
Sbjct: 306 NRSVWENIHQKAREFCFFDDYNWDITMWATVFPSFGSPVYTLRGPRTSAVHFGKCGLHQG 365

Query: 365 QGDRDVCIDNGVLNIDVKDIDKIANINSEWRLDVFRDQPGYGAGFKGWGGWGDERDRQLC 424
           +GD   CIDNGV+NI+VK+ DK+ NI   W + V++ Q GY AGF+GWGGWGD+RDR LC
Sbjct: 366 RGDEGDCIDNGVVNIEVKETDKVVNIKEGWGVRVYKHQAGYKAGFEGWGGWGDDRDRHLC 425

Query: 425 LNFAHMYHSAPNS 431
           L+FA MY  + +S
Sbjct: 426 LDFATMYRYSSSS 427

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888419.19.2e-25093.58alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Benincas... [more]
XP_008462069.11.2e-24993.35PREDICTED: alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferas... [more]
XP_004144546.17.8e-24190.37alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucumis ... [more]
XP_022969101.15.8e-22886.05alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucurbit... [more]
KAG7011860.19.9e-22886.28Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, partial ... [more]
Match NameE-valueIdentityDescription
Q9FT884.8e-16161.43Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Arabid... [more]
Q104692.5e-4832.22Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Homo s... [more]
O190712.1e-4731.55Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Sus sc... [more]
Q093262.1e-4731.28Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Rattus... [more]
Q921V58.0e-4731.49Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Mus mu... [more]
Match NameE-valueIdentityDescription
A0A5A7UTF05.8e-25093.35Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumi... [more]
A0A1S3CG235.8e-25093.35Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumi... [more]
A0A0A0K3433.8e-24190.37Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumi... [more]
A0A6J1HWS52.8e-22886.05Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucurb... [more]
A0A6J1GLB73.1e-22786.05Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucurb... [more]
Match NameE-valueIdentityDescription
AT2G05320.13.4e-16261.43beta-1,2-N-acetylglucosaminyltransferase II [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007754N-acetylglucosaminyltransferase IIPFAMPF05060MGAT2coord: 89..417
e-value: 9.1E-99
score: 330.8
IPR007754N-acetylglucosaminyltransferase IIPANTHERPTHR12871BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE IIcoord: 6..424
IPR029044Nucleotide-diphospho-sugar transferasesGENE3D3.90.550.10Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain Acoord: 101..361
e-value: 6.1E-27
score: 96.6
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILY53448Nucleotide-diphospho-sugar transferasescoord: 100..421
NoneNo IPR availablePANTHERPTHR12871:SF0ALPHA-1,6-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASEcoord: 6..424

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10005503.1HG10005503.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009312 oligosaccharide biosynthetic process
biological_process GO:0006487 protein N-linked glycosylation
cellular_component GO:0000139 Golgi membrane
cellular_component GO:0005795 Golgi stack
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008455 alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity
molecular_function GO:0046872 metal ion binding