HG10005493 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10005493 vs. NCBI nr
Match: XP_038888691.1 (calcium-transporting ATPase 12, plasma membrane-type-like [Benincasa hispida])

HSP 1 Score: 1674.4 bits (4335), Expect = 0.0e+00
Identity = 867/990 (87.58%), Postives = 915/990 (92.42%), Query Frame = 0

Query: 1   MFQGTDDDGGAQPLLVVSAVATSYKPAGFKFRQLVLSIRFVLCLNRTRSPQPLLHTVINV 60
           MFQGT DDGGAQPLLVVS V TSYKPAG KFRQLVLSIRFVLCLNRTRSP   LHTVIN+
Sbjct: 1   MFQGT-DDGGAQPLLVVSDVGTSYKPAGVKFRQLVLSIRFVLCLNRTRSPAS-LHTVINI 60

Query: 61  IPSESERNNKKQRLKLLVKDKDLEALHNFGGVQEAVSFLRSESEPPIDVVGDLAQTVHGL 120
           I SESE   KKQRLK LVK+K+LEAL++FGGVQEAVSFL+SESEPPI VVGDLAQTVHGL
Sbjct: 61  ISSESESEIKKQRLKRLVKEKNLEALNDFGGVQEAVSFLQSESEPPIGVVGDLAQTVHGL 120

Query: 121 GFWGSLLLFVKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGLKHGWHDGVGILLAV 180
           GFWGS +LFVKGFW CLYNSLNSCTILVLVIAADLSFAIGSLEQGL+HGWHDGVGILLAV
Sbjct: 121 GFWGSFILFVKGFWSCLYNSLNSCTILVLVIAADLSFAIGSLEQGLEHGWHDGVGILLAV 180

Query: 181 FLLVFVPSISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFDVKEGEIIHLKKGDR 240
           FLLV VPS+SS Y+KK +EK+LLKIKNN+EVTVKRHEILQ VSVFDVKEGEIIHLKKGDR
Sbjct: 181 FLLVIVPSVSSFYQKKRQEKKLLKIKNNVEVTVKRHEILQGVSVFDVKEGEIIHLKKGDR 240

Query: 241 VPADGLLIKGKNLILDEVINSHIDPHRNPFLFSGSVVEYGEGEMIAVSIDHDTAFRKGLL 300
           VPADGLLIKG+NLILDE INSHIDPHRNPFLFSGSVVEYG+GEMIAVSIDHDTAFRKGLL
Sbjct: 241 VPADGLLIKGENLILDEAINSHIDPHRNPFLFSGSVVEYGKGEMIAVSIDHDTAFRKGLL 300

Query: 301 DVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLLREKHKHGDYYNDKPETK 360
           DVIVHPSQE LFQSRINKPYEFIEKFSLVVSLM+LLVVL RLL EKHKH DYYNDKPE+K
Sbjct: 301 DVIVHPSQETLFQSRINKPYEFIEKFSLVVSLMLLLVVLTRLLCEKHKHDDYYNDKPESK 360

Query: 361 GKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAIAVSLSFWREKMRRS 420
           GKLTV FVA  F RM  EFGKFRVS VAT LLTM+IGIQHGMPLAI +SLS WRE+MRRS
Sbjct: 361 GKLTVGFVANAFERMSFEFGKFRVSLVATVLLTMIIGIQHGMPLAITISLSLWRERMRRS 420

Query: 421 LKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKINPGMEFHPDIHQGFE 480
            KV CRNLSACGTLGLVS ICID+TAEFSFHEVE+RE FVGEEKINPGMEFHPDIHQGFE
Sbjct: 421 HKVKCRNLSACGTLGLVSVICIDVTAEFSFHEVEVRELFVGEEKINPGMEFHPDIHQGFE 480

Query: 481 VVARVLRFDPKTFVLLRNDLLLNFWGESGLEINKESLDQRFDIIEHKFLSSEKGIGVLVN 540
           V ARVL  DP T VLLRN+ LLNFW +SGL+INKES DQRFD I+HKFLSSEKGIGVL N
Sbjct: 481 VAARVLCLDPNTSVLLRNN-LLNFWEKSGLKINKESPDQRFDFIDHKFLSSEKGIGVLRN 540

Query: 541 KSRGDAAANLFHEHFYGDASTLLKLCSNYYDIRGRIHDIENRKDVFEKVIREMEEKGLRP 600
           KS GD  ANLFH+HFYG+ASTLL +CSNYYDIRGRIHDIENRKDVF+K++REMEE+GLRP
Sbjct: 541 KSIGDTEANLFHDHFYGNASTLLNMCSNYYDIRGRIHDIENRKDVFQKMVREMEERGLRP 600

Query: 601 IAFACKQTNDHQVFEGELKLLGLMGLKFSHEQILLALKDLENIGVRIILTSEDELSVAIN 660
           IAFACKQTNDHQVFEGELKLLGLMGLKFSHE+ILLALKDLENIGVRIILTSEDELSVAIN
Sbjct: 601 IAFACKQTNDHQVFEGELKLLGLMGLKFSHEKILLALKDLENIGVRIILTSEDELSVAIN 660

Query: 661 KADGLGAQCDPNNKEVEGPRFREIMKINGREKNELMKSITVMGKATSEDKLLLVRELKAK 720
             DGLG Q DPN +E+EG RFREIMKI+G EKNELMKSITVMGKATSEDKLLLV+ELKAK
Sbjct: 661 MTDGLGVQYDPNKQEIEGERFREIMKIDGMEKNELMKSITVMGKATSEDKLLLVKELKAK 720

Query: 721 GETVALLGGLTSGDVPTLMEADIGIVQENRSTKVSRLVSDLSCEDVTSLNHTLKYGRSNY 780
           GETVALLGGLTSGDVPTL+EADIGIVQENRSTKVSRLVSDLSCEDVTSLNHTLKYGRS Y
Sbjct: 721 GETVALLGGLTSGDVPTLIEADIGIVQENRSTKVSRLVSDLSCEDVTSLNHTLKYGRSYY 780

Query: 781 LNIQKFYEVQLTALISGLLITLVCTMVSGKSPITTFHLTWVTLIMCLLGSLMMMMELNDE 840
           LNIQKFY+VQLTALISGLLI L+CTMVSGKSPITTFHL WVTLIMCLLGSLMM+MELND 
Sbjct: 781 LNIQKFYQVQLTALISGLLINLICTMVSGKSPITTFHLIWVTLIMCLLGSLMMVMELNDA 840

Query: 841 EVRNPVGGGNREQSLITRVILKKIVIHVLCQALVFLLLEYLGQKIMPHMEEDVRHTMIFN 900
           EVRN VGG +REQSLITRVILKKIVIHVLCQALVFL+LEYLGQKIMPHMEEDVR+TMIFN
Sbjct: 841 EVRNRVGGSDREQSLITRVILKKIVIHVLCQALVFLMLEYLGQKIMPHMEEDVRNTMIFN 900

Query: 901 TFILCQISNLLGAITVGLVTNEVVDLQVVLHILWVMISVVGVLAVQVMVIEFHGTIVNAV 960
           TFILCQI+NLLGAIT+GLVTNEV    VVLHILWVMISVV VLAVQVMVIEF GTIVN V
Sbjct: 901 TFILCQIANLLGAITMGLVTNEVAVFHVVLHILWVMISVVSVLAVQVMVIEFDGTIVNGV 960

Query: 961 KLSAVQWTICFLFALALGWASNIFFHFLLH 991
           KLSAVQW ICFL ALALGWAS IFFHF+LH
Sbjct: 961 KLSAVQWIICFLLALALGWASYIFFHFVLH 987

BLAST of HG10005493 vs. NCBI nr
Match: XP_004144617.1 (calcium-transporting ATPase 12, plasma membrane-type [Cucumis sativus] >KAE8645712.1 hypothetical protein Csa_020442 [Cucumis sativus])

HSP 1 Score: 1384.4 bits (3582), Expect = 0.0e+00
Identity = 745/1029 (72.40%), Postives = 837/1029 (81.34%), Query Frame = 0

Query: 1    MFQGTDDD--GGAQPLLVVSAVA----TSYKPAGFK-FRQLVLSIRFVL-CLNRTRSPQP 60
            MF+G  DD  GGAQPLL+ S  A    TS KPAGFK  +Q+VLSIRF+L CLNRTRSP P
Sbjct: 1    MFEGGTDDNGGGAQPLLLASLSAPTTTTSNKPAGFKLIQQIVLSIRFLLICLNRTRSPLP 60

Query: 61   LLHTVI--------------NVIPS------------ESERNNKKQRLKLLVKDKDLEAL 120
            LLHT I              ++IPS            E ER  KK RLK +VK+K+L AL
Sbjct: 61   LLHTPIDDIIPLEEERAVIDDIIPSEEEKEVIDIARLEKEREMKKDRLKEIVKEKNLAAL 120

Query: 121  H-NFGGVQEAVSFLRSESEPPIDVVGDLAQTVHGLGFWGSLLLFVKGFWLCLYNSLNSCT 180
              +F GV EAVSFL S+ +  ID +GDLAQT    GFW SLLLF+K FW  LYNS NS T
Sbjct: 121  ETDFCGVGEAVSFLHSQWDTQIDAIGDLAQT----GFWDSLLLFLKAFWSSLYNSFNSST 180

Query: 181  ILVLVIAADLSFAIGSLEQGLKHGWHDGVGILLAVFLLVFVPSISSLYRKKAEEKRLLKI 240
            IL+LV AA  S AIGS+EQGLKHGWHD VGILLAVFLL+F  S+    +KKAEEK+ LKI
Sbjct: 181  ILLLVFAAGFSLAIGSIEQGLKHGWHDCVGILLAVFLLLFFSSVLGFCKKKAEEKKRLKI 240

Query: 241  KNNLEVTVKRHEILQRVSVFDVKEGEIIHLKKGDRVPADGLLIKGKNLILDEVINSHIDP 300
            KNN +VTVKRHE LQ +SVFDVKEGEIIHLKKGDRV ADGLLIKG NLILDE INSHIDP
Sbjct: 241  KNNFKVTVKRHEELQEISVFDVKEGEIIHLKKGDRVLADGLLIKGNNLILDEAINSHIDP 300

Query: 301  HRNPFLFSGSVVEYGEGEMIAVSIDHDTAFRKGLLDVIVHPSQEMLFQSRINKPYEFIEK 360
             RNPFL SGSVVEYGEGEM+AVSID DTAF KGLLDVIV+PSQE LFQSRINKPYEF EK
Sbjct: 301  RRNPFLLSGSVVEYGEGEMLAVSIDRDTAFWKGLLDVIVYPSQETLFQSRINKPYEFFEK 360

Query: 361  FSLVVSLMMLLVVLVRLLREKHKHGDYYNDKPETKGKLTVDFVAKIFGRMFLEFGKFRVS 420
            FSL + LM+LLVVL RLL EKH+HG+YYNDKPETKGKLTV F+   F RM   FGK+RVS
Sbjct: 361  FSLGLYLMVLLVVLTRLLCEKHEHGNYYNDKPETKGKLTVAFIGNAFQRMSFVFGKYRVS 420

Query: 421  TVATFLLTMVIGIQHGMPLAIAVSLSFWREKMRRSLKVNCRNLSACGTLGLVSAICIDIT 480
            +VAT + TMV+GIQHGMPLAI  SLSFWREKMRRS KVNC+NLSACGTLGLVS ICID+ 
Sbjct: 421  SVATVVFTMVVGIQHGMPLAITFSLSFWREKMRRSHKVNCQNLSACGTLGLVSVICIDVA 480

Query: 481  AEFSFHEVEIREFFVGEEKINPGMEFHPDIHQGFEVVARVLRFDPKTFVLLRNDLLLNFW 540
            AE SF +VE+ EF+VGEEKINPGMEFH D+HQGFE  +RVLR DPKT  L  ++ LL+FW
Sbjct: 481  AELSFCDVEVDEFYVGEEKINPGMEFHLDVHQGFEAASRVLRIDPKTTFL--SEYLLDFW 540

Query: 541  GESGLEINKE-SLDQRFDIIEHKFLSSEKGIGVLVNKSR-GDAAANLFHEHFYGDASTLL 600
              SGL+IN E  LDQ FDII+HKFLSSE+ +GVLVNK+R GD  +NL HEHFYGDAST+L
Sbjct: 541  KNSGLKINNEPDLDQMFDIIDHKFLSSEESMGVLVNKTRGGDTVSNLIHEHFYGDASTIL 600

Query: 601  KLCSNYYDIRGRIHDIENRKDVFEKVIREMEEKGLRPIAFACKQTNDHQVFEGELKLLGL 660
             +CSNYYDI GR+HDIENR DV +  I+EMEEKGLRPIAFACKQ ND   FEGELKLLG 
Sbjct: 601  NMCSNYYDIGGRVHDIENRNDVLQTKIKEMEEKGLRPIAFACKQKNDQTGFEGELKLLGY 660

Query: 661  MGLKFSHEQILLALKDLENIGVRIILTSEDELSVAINKADGLGAQCDPNNKEVEG-PRFR 720
            MGLK SHE+I  ALKDL+NIG+RIILTS+D +SV I  A  LG +CDPNN++ EG  R R
Sbjct: 661  MGLKISHEKIQHALKDLQNIGIRIILTSKDNVSVIIKMAGDLGTRCDPNNRQTEGDQRLR 720

Query: 721  EI-MKINGREKNELMKSITVMGKATSEDKLLLVRELKAKGETVALLGGLTSGDVPTLMEA 780
            EI MK N REKNELMKSIT MGKATS+DKL+LV+ELKAKGETVA +GGLTSGDVPTL+EA
Sbjct: 721  EILMKNNEREKNELMKSITFMGKATSDDKLVLVKELKAKGETVAFIGGLTSGDVPTLIEA 780

Query: 781  DIGIVQENRSTKVSRLVSDLSCEDVTSLNHTLKYGRSNYLNIQKFYEVQLTALISGLLIT 840
            DIGI+QENRSTK  +LVSDL  EDVTSLNHTLKYGRSNYLNI+KFY++QLTALISGLLIT
Sbjct: 781  DIGIIQENRSTKECKLVSDLRYEDVTSLNHTLKYGRSNYLNIKKFYQLQLTALISGLLIT 840

Query: 841  LVCTMVSGKSPITTFHLTWVTLIMCLLGSLMMMMELNDEEVRNPVGGGNREQSLITRVIL 900
            L+CTMVSGKSPIT+FHLTWVTLI CLLG LMM+MELNDEEV+N VGG +R Q+LITR I+
Sbjct: 841  LICTMVSGKSPITSFHLTWVTLITCLLGGLMMVMELNDEEVQNVVGGSDRNQALITRDIV 900

Query: 901  KKIVIHVLCQALVFLLLEYLGQKIMPHMEEDVRHTMIFNTFILCQISNLLGAITVGLVTN 960
            KKIVIHVLCQA VFL++EYLG KI+P M+EDVR TMIFNT+ILCQI+NLLGAI+VGLVTN
Sbjct: 901  KKIVIHVLCQASVFLIIEYLGHKIVPQMKEDVRDTMIFNTYILCQIANLLGAISVGLVTN 960

Query: 961  EVVDLQVVLHILWVMISVVGVLAVQVMVIEFHGTIVNAVKLSAVQWTICFLFALALGWAS 991
                 QV + ILWV+I VVGVLAVQV+VIE HGTIVN VKLSA+QW ICFLFALALGWAS
Sbjct: 961  RAAVFQVAVQILWVLIFVVGVLAVQVVVIELHGTIVNGVKLSALQWIICFLFALALGWAS 1020

BLAST of HG10005493 vs. NCBI nr
Match: XP_008462128.1 (PREDICTED: calcium-transporting ATPase 12, plasma membrane-type-like [Cucumis melo])

HSP 1 Score: 1275.0 bits (3298), Expect = 0.0e+00
Identity = 667/890 (74.94%), Postives = 746/890 (83.82%), Query Frame = 0

Query: 104 EPP-IDVVGDLAQTVHGLGFWGSLLLFVKGFWLCLYNSLNSCTILVLVIAADLSFAIGSL 163
           EPP  D +GDLAQTV GLG  GSLLLFVKGFW CLYNSLNSCTIL+LVIAA  S AIGS+
Sbjct: 3   EPPDNDAIGDLAQTVGGLGLRGSLLLFVKGFWSCLYNSLNSCTILLLVIAAGFSLAIGSI 62

Query: 164 EQGLKHGWHDGVGILLAVFLLVFVPSISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRV 223
           EQGLK GWHD VGILLA+FLLVF  S+ S  +KKAEEK+ LKIKNNL+VTVKRHE L  +
Sbjct: 63  EQGLKDGWHDCVGILLAIFLLVFFSSVLSFCKKKAEEKKRLKIKNNLKVTVKRHEELVEI 122

Query: 224 SVFDVKEGEIIHLKKGDRVPADGLLIKGKNLILDEVINSHIDPHRNPFLFSGSVVEYGEG 283
           SVFDVKEGEIIHLKKGD V ADGLL KGKN+ILDE INSHIDPHRNPFLFSGSVVEYGEG
Sbjct: 123 SVFDVKEGEIIHLKKGDHVLADGLLTKGKNMILDEAINSHIDPHRNPFLFSGSVVEYGEG 182

Query: 284 EMIAVSIDHDTAFRKGLLDVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRL 343
           EMIAVSI+ DTAF KGLLDVIVHPSQE LFQSRINKPYEFIEKFSLV+ LM+LLVVL+RL
Sbjct: 183 EMIAVSINRDTAFWKGLLDVIVHPSQETLFQSRINKPYEFIEKFSLVMYLMVLLVVLIRL 242

Query: 344 LREKHKHGDYYNDKPETKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGM 403
           L +K++HG+YYNDKPE KGKLTV FV   F RM   F K+RVS+VAT +L +V+GIQHGM
Sbjct: 243 LCKKNEHGNYYNDKPENKGKLTVAFVGNAFERMSFVFAKYRVSSVATAVLIIVVGIQHGM 302

Query: 404 PLAIAVSLSFWREKMRRSLKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGE 463
           PLAI VSL FWREKMRRS KVNC+NLSACGTLGLVS IC+DITAE SF++VE+ EF+VGE
Sbjct: 303 PLAITVSLFFWREKMRRSHKVNCQNLSACGTLGLVSVICVDITAELSFYDVEVDEFYVGE 362

Query: 464 EKINPGMEFHPDIHQGFEVVARVLRFDPKTFVLLRNDLLLNFWGESGLEINKE-SLDQRF 523
           EKINPGMEFH DIH+G E  + VLRFDPKT V L N  LL+FW  SGL+IN E  LD+ F
Sbjct: 363 EKINPGMEFHLDIHRGIEAASGVLRFDPKTTVRLENK-LLDFWENSGLKINNEPDLDEMF 422

Query: 524 DIIEHKFLSSEKGIGVLVNKSR-GDAAANLFHEHFYGDASTLLKLCSNYYDIRGRIHDIE 583
           DII+HKFLSSEKGIGVLV K+R G+  ANL HE+FYGDAST+L +CSNYYD+ GRIHDIE
Sbjct: 423 DIIDHKFLSSEKGIGVLVYKTRGGETGANLIHENFYGDASTILNMCSNYYDMGGRIHDIE 482

Query: 584 NRKDVFEKVIREMEEKGLRPIAFACKQTNDHQVFEGELKLLGLMGLKFSHEQILLALKDL 643
           NRKDV EK IREMEEKGLRPIAFA   TND  VFEGELKLLG MGLK S +++L ALKDL
Sbjct: 483 NRKDVLEKKIREMEEKGLRPIAFAGNHTNDQTVFEGELKLLGYMGLKISQQEVLRALKDL 542

Query: 644 ENIGVRIILTSEDELSVAINKADGLGAQCDPNNKEVEGPRFREIMKINGREKNELMKSIT 703
           +N G+RIILTSED+LS  IN AD LG +CDPNNKE+EG RFREIMKI+G +KNELMKSIT
Sbjct: 543 KNNGIRIILTSEDKLSTIINMADDLGIRCDPNNKEIEGERFREIMKIDGMDKNELMKSIT 602

Query: 704 VMGKATSEDKLLLVRELKAKGETVALLGGLTSGDVPTLMEADIGIVQENRSTKVSRLVSD 763
           +MGKATS+DKL+LV+ELKA  E VA +GGLTS D+PTL+EADIGI+QENRSTK  +LVSD
Sbjct: 603 LMGKATSDDKLVLVKELKANREVVAFIGGLTSEDLPTLIEADIGIIQENRSTKECKLVSD 662

Query: 764 LSCEDVTSLNHTLKYGRSNYLNIQKFYEVQLTALISGLLITLVCTMVSGKSPITTFHLTW 823
           LS EDVTSLNHTLKYGR NYLNIQKFY+VQL A ISGLLITL+CT+VSGKSPIT+FHLTW
Sbjct: 663 LSYEDVTSLNHTLKYGRINYLNIQKFYQVQLAASISGLLITLICTIVSGKSPITSFHLTW 722

Query: 824 VTLIMCLLGSLMMMMELNDEEVRNPVGGGNREQSLITRVILKKIVIHVLCQALVFLLLEY 883
           +TLIMCLLGSLMM+ME +DEEVRN VGGG+R Q+LITRV LKKIVIH LCQA VFL+LEY
Sbjct: 723 MTLIMCLLGSLMMVMESSDEEVRNLVGGGDRNQTLITRVTLKKIVIHALCQASVFLILEY 782

Query: 884 LGQKIMPHMEEDVRHTMIFNTFILCQISNLLGAITVGLVTNEVVDLQVVLHILWVMISVV 943
           +G KI+P M+EDV+ TMIFNTFILCQ++NLLGAITVGL                      
Sbjct: 783 VGHKIVPQMKEDVKETMIFNTFILCQMANLLGAITVGL---------------------- 842

Query: 944 GVLAVQVMVIEFHGTIVNAVKLSAVQWTICFLFALALGWASNIFFHFLLH 991
                 V+VIE  GTIVN VKLSA+QW ICFLFA ALGWAS IFFHF+LH
Sbjct: 843 ------VVVIEVDGTIVNGVKLSALQWIICFLFASALGWASYIFFHFVLH 863

BLAST of HG10005493 vs. NCBI nr
Match: KAA0059193.1 (calcium-transporting ATPase 12, plasma membrane-type-like [Cucumis melo var. makuwa])

HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 654/885 (73.90%), Postives = 736/885 (83.16%), Query Frame = 0

Query: 108 DVVGDLAQTVHGLGFWGSLLLFVKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGLK 167
           D +GDLA+TV GLG  GSLLLFVKGFW CLYNSLNSCTIL+LVIAA+ S AIGS+EQGLK
Sbjct: 5   DAIGDLAETVGGLGLRGSLLLFVKGFWSCLYNSLNSCTILLLVIAAEFSLAIGSIEQGLK 64

Query: 168 HGWHDGVGILLAVFLLVFVPSISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFDV 227
            GWHD VGILLA+FLLVF  S+ S  +KKAEEK+ LKIKNNL+VTVKRHE L  +SVFDV
Sbjct: 65  DGWHDCVGILLAIFLLVFFSSVLSFCKKKAEEKKRLKIKNNLKVTVKRHEELVEISVFDV 124

Query: 228 KEGEIIHLKKGDRVPADGLLIKGKNLILDEVINSHIDPHRNPFLFSGSVVEYGEGEMIAV 287
           KEGEIIHLKKGD V ADGLL KGKN+ILDE INSHIDPHRNPFLFSGSVVEYGEGEMIAV
Sbjct: 125 KEGEIIHLKKGDHVLADGLLTKGKNMILDEAINSHIDPHRNPFLFSGSVVEYGEGEMIAV 184

Query: 288 SIDHDTAFRKGLLDVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLLREKH 347
           SI+ DTAF KGLLDVIVHPSQE LFQSRINKPYEFIEKFSLV+ LM+LLVVL+RLL +K+
Sbjct: 185 SINRDTAFWKGLLDVIVHPSQETLFQSRINKPYEFIEKFSLVMYLMVLLVVLIRLLCKKN 244

Query: 348 KHGDYYNDKPETKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAIA 407
           +HG+YYNDKPE KGKLTV FV   F RM   FGK+RVS+VAT +L +V+GIQHGMPLAI 
Sbjct: 245 EHGNYYNDKPENKGKLTVAFVGNAFERMSFVFGKYRVSSVATAVLIIVVGIQHGMPLAIT 304

Query: 408 VSLSFWREKMRRSLKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKINP 467
           VSL FWRE      K++   LS                         + EF+VGEEKINP
Sbjct: 305 VSLFFWRE----DEKISQSKLSE-----------------------SVDEFYVGEEKINP 364

Query: 468 GMEFHPDIHQGFEVVARVLRFDPKTFVLLRNDLLLNFWGESGLEINKE-SLDQRFDIIEH 527
           GMEFH DIH+G E  +RVLRFDPKT V L N  LL+FW  SGL+IN E  LD+ FDII+H
Sbjct: 365 GMEFHLDIHRGIEAASRVLRFDPKTTVRLENK-LLDFWENSGLKINNEPDLDEMFDIIDH 424

Query: 528 KFLSSEKGIGVLVNKSR-GDAAANLFHEHFYGDASTLLKLCSNYYDIRGRIHDIENRKDV 587
           KFLSSEKGIGVLV K+R G+  ANL HE+FYGDAST+L +CSNYYD+ GRIHDIENRKDV
Sbjct: 425 KFLSSEKGIGVLVYKTRGGETGANLIHENFYGDASTILNMCSNYYDMGGRIHDIENRKDV 484

Query: 588 FEKVIREMEEKGLRPIAFACKQTNDHQVFEGELKLLGLMGLKFSHEQILLALKDLENIGV 647
            EK IREMEEKGLRPIAFA   TND  VFEGELKLLG MGLK S +++L ALKDL+N G+
Sbjct: 485 LEKKIREMEEKGLRPIAFARNHTNDQTVFEGELKLLGYMGLKISQQEVLRALKDLKNNGI 544

Query: 648 RIILTSEDELSVAINKADGLGAQCDPNNKEVEGPRFREIMKINGREKNELMKSITVMGKA 707
           RIIL SED+LS  IN AD LG +CDPNNKE+EG RFREIMKI+G +KNELMKSIT+MGKA
Sbjct: 545 RIILISEDKLSTIINMADDLGIRCDPNNKEIEGERFREIMKIDGMDKNELMKSITLMGKA 604

Query: 708 TSEDKLLLVRELKAKGETVALLGGLTSGDVPTLMEADIGIVQENRSTKVSRLVSDLSCED 767
           TS+DKL+LV+ELKA  E VA +GGLTS D+PTL+EADIGI+QENRSTK  +LVSDLS ED
Sbjct: 605 TSDDKLVLVKELKANREVVAFIGGLTSEDLPTLIEADIGIIQENRSTKECKLVSDLSYED 664

Query: 768 VTSLNHTLKYGRSNYLNIQKFYEVQLTALISGLLITLVCTMVSGKSPITTFHLTWVTLIM 827
           VTSLNHTLKYGR NYLNIQKFY+VQL A ISGLLITL+CT+VSGKSPIT+FHLTW+TLIM
Sbjct: 665 VTSLNHTLKYGRINYLNIQKFYQVQLAASISGLLITLICTIVSGKSPITSFHLTWMTLIM 724

Query: 828 CLLGSLMMMMELNDEEVRNPVGGGNREQSLITRVILKKIVIHVLCQALVFLLLEYLGQKI 887
           CLLGSLMM+ME +DEEVRN VGGG+R Q+LITRV LKKIVIH LCQA VFL+LEY+G KI
Sbjct: 725 CLLGSLMMVMESSDEEVRNLVGGGDRNQTLITRVTLKKIVIHALCQASVFLILEYVGHKI 784

Query: 888 MPHMEEDVRHTMIFNTFILCQISNLLGAITVGLVTNEVVDLQVVLHILWVMISVVGVLAV 947
           +P M+EDV+ TMIFNTFILCQ++NLLGAITVGLVTN+    QV LHI WV+ISVVGVLA+
Sbjct: 785 VPQMKEDVKETMIFNTFILCQMANLLGAITVGLVTNKAAVFQVALHIFWVLISVVGVLAL 844

Query: 948 QVMVIEFHGTIVNAVKLSAVQWTICFLFALALGWASNIFFHFLLH 991
           QV+VIE  GTIVN VKLSA+QW ICFLFA ALGWAS IFFHF+LH
Sbjct: 845 QVVVIEVDGTIVNGVKLSALQWIICFLFASALGWASYIFFHFVLH 861

BLAST of HG10005493 vs. NCBI nr
Match: XP_023554362.1 (putative calcium-transporting ATPase 13, plasma membrane-type [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1216.8 bits (3147), Expect = 0.0e+00
Identity = 674/979 (68.85%), Postives = 773/979 (78.96%), Query Frame = 0

Query: 1   MFQGTDDDGGAQPLLVVSAVATSYKPAGFKFRQLVLSIRFVLCLNRTRSPQPLLHTVINV 60
           MFQ T DD GAQPLL    V T  K    +FRQLVLSIRFVL L RT SP P  +TVI+V
Sbjct: 3   MFQRTADD-GAQPLL----VGTPSKLV--RFRQLVLSIRFVLSLRRTSSPPPPQYTVIDV 62

Query: 61  IPSESERNNKKQRLKLLVKDKDLEALHNFGGVQEAVSFLRSESEPPIDVVGDLAQTVHGL 120
              E ER+ +KQRLK +VK+K+L+AL +FGGV+EAVSFLRSES   ID     AQT+ G 
Sbjct: 63  -HFEDERDIQKQRLKKIVKEKNLKALKDFGGVEEAVSFLRSESLLQID-----AQTLRGY 122

Query: 121 GFWGSLLLFVKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGLKHGWHDGVGILLAV 180
           GF GS LLFVK F   L+NSLNS TIL LVIAA LSFAI SLEQGLKHGWHDG GILLAV
Sbjct: 123 GFCGSFLLFVKEFCCYLWNSLNSWTILFLVIAAGLSFAIESLEQGLKHGWHDGFGILLAV 182

Query: 181 FLLVFVPSISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFDVKEGEIIHLKKGDR 240
           FLLVF P I S+YRK+AEEK LLK KN LEVTVKR E+ QRVSV DV+EG+ IHL KGDR
Sbjct: 183 FLLVFFPPILSVYRKRAEEKELLKNKNILEVTVKRDELCQRVSVSDVREGDRIHLNKGDR 242

Query: 241 VPADGLLIKGKNLILDEVINSHIDPHRNPFLFSGSVVEYGEGEMIAVSIDHDTAFRKGLL 300
           VPADGLLIKGKNLILDEVINSHIDP+RNPFLF GSVV++GEGEMIAVS+ HDTAF K LL
Sbjct: 243 VPADGLLIKGKNLILDEVINSHIDPNRNPFLFCGSVVDHGEGEMIAVSVGHDTAFGKVLL 302

Query: 301 DVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLLREKHKHGDYYNDKPETK 360
           +++ HPSQE LFQSR+NKPY+F+EKFSL VSL +L+VVL RLL  KH H DYYND+PETK
Sbjct: 303 ELVTHPSQETLFQSRMNKPYQFLEKFSLTVSLTILVVVLARLLC-KH-HDDYYNDRPETK 362

Query: 361 GKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAIAVSLSFWREKMRRS 420
           GKLT   V + F RMFL+FG  R S +AT LLTMVIG+QHGMP AI +SLSFWREKM RS
Sbjct: 363 GKLTAGIVVQAFERMFLKFGS-RASFLATVLLTMVIGLQHGMPFAITLSLSFWREKM-RS 422

Query: 421 LKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKINPGMEFHPDIHQGFE 480
           LK NC NLSACGTLGLVSAICIDITAE SFHEVE+ EFFVGE+KIN  MEFH DI Q  E
Sbjct: 423 LKGNCLNLSACGTLGLVSAICIDITAELSFHEVELCEFFVGEQKINSAMEFHTDISQSLE 482

Query: 481 VVARVLRFDPKTFVLLRNDLLLNFWGESGLEINKESLDQRFDIIEHKFLSSEKGIGVLVN 540
           + A++L  DPK  V L NDL+     + G      + DQ+FDII+HKFLSSEKGIG LV+
Sbjct: 483 LAAQILYSDPKVSVHLGNDLVHFLKNKLG------APDQKFDIIDHKFLSSEKGIGALVS 542

Query: 541 K-SRGDAAANLFHEHFYGDASTLLKLCSNYYDIRGRIHDIENRKDVFEKVIREMEEKGLR 600
           K S  D  ANLFH H+YGDAS+++ +CS YYDIRG +HDIEN KDVFE VIREM+++GLR
Sbjct: 543 KRSEMDTEANLFHVHYYGDASSIVSMCSQYYDIRGIVHDIENEKDVFENVIREMKQRGLR 602

Query: 601 PIAFACKQ-TNDHQVFEGELKLLGLMGLKFSHEQILLALKDLENIGVRIILTSEDELSVA 660
           PIAFACKQ  ND Q+FEGE KLLG +GLKFSHE+ L AL+DL+NIG RIIL SEDEL  A
Sbjct: 603 PIAFACKQRRNDDQLFEGESKLLGFIGLKFSHEKTLNALRDLQNIGARIILISEDELFEA 662

Query: 661 INKADGLGAQCDPNNKEVEGPRFREIMKINGREKNELMKSITVMGKATSEDKLLLVRELK 720
           IN  +GLG QCD  N  VEG RF+EIM  +G E++ELMK+I+ MGKATSEDK LL++ELK
Sbjct: 663 INMVNGLGTQCDLKNNVVEGERFKEIMMTDGTERHELMKNISFMGKATSEDKHLLIKELK 722

Query: 721 AKGETVALLGGLTSGDVPTLMEADIGIVQENRSTKVSRLVSDLSCEDVTSLNHTLKYGRS 780
           A+G  VA    LTS DVPTLMEADI I+QE+RS+K SR+V D+ CEDVTSLN TLK  R 
Sbjct: 723 AEGNIVAFSPDLTSRDVPTLMEADIRILQEHRSSKESRMVGDIMCEDVTSLNQTLKSSRC 782

Query: 781 NYLNIQKFYEVQLTALISGLLITLVCTMVSGKSPITTFHLTWVTLIMCLLGSLMMMMELN 840
            YLNIQKFY++QL A +SGL+ITLVCTMVSGKSPI T  L WVTLIMCLLG LMM+MELN
Sbjct: 783 IYLNIQKFYQIQLIASVSGLVITLVCTMVSGKSPIATLQLIWVTLIMCLLGGLMMVMELN 842

Query: 841 DEEVRNPVGGGNREQSLITRVILKKIVIHVLCQALVFLLLEYLGQKIMPHMEEDVRHTMI 900
           DEEV+ P+ G +R QSLIT VIL KIVIHVLCQ  VFLL EYLGQ++MPHMEE+VRHTM+
Sbjct: 843 DEEVQAPLQGRDRNQSLITEVILYKIVIHVLCQVFVFLLFEYLGQEVMPHMEENVRHTMM 902

Query: 901 FNTFILCQISNLLGAITVGLVTNEVVDLQVVLHILWVMISVVGVLAVQVMVIEFHGTIVN 960
           FNTF+LCQI+NLL A  +GLVTN     + VL   W ++S+  VLAVQ++VIEF G +VN
Sbjct: 903 FNTFVLCQIANLLAA--MGLVTNRGAFFKAVLQSPWFLVSLATVLAVQMVVIEFAGGVVN 956

Query: 961 AVKLSAVQWTICFLFALAL 978
            VKLSAVQW+ CF FA  L
Sbjct: 963 GVKLSAVQWSSCFFFASLL 956

BLAST of HG10005493 vs. ExPASy Swiss-Prot
Match: Q9LY77 (Calcium-transporting ATPase 12, plasma membrane-type OS=Arabidopsis thaliana OX=3702 GN=ACA12 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 1.1e-99
Identity = 295/966 (30.54%), Postives = 495/966 (51.24%), Query Frame = 0

Query: 58   INVIPSESERNNKKQRLKLLVKDKDLEALHNFGGVQEAVSFLRSESEPPIDVVGDLAQTV 117
            IN +P        +++L  ++K KDL  +   GGV+   + LR  + P   + G+  +  
Sbjct: 77   INSMPLSYVPAIDQEQLVEIMKGKDLPGIQALGGVEGVAASLR--TNPTKGIHGNEQEVS 136

Query: 118  HGLGFWGSLLLF---VKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGLKHGWHDGV 177
                 +GS        KG    +Y +    TIL+L++ A  S   G  E G+K GW++G 
Sbjct: 137  RRRDLFGSNTYHKPPPKGLLFFVYEAFKDLTILILLVCAIFSLGFGIKEHGIKEGWYEGG 196

Query: 178  GILLAVFLLVFVPSISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFDVKEGEIIH 237
             I +AVFL++ V ++S+ +R++ +  +L KI NN++V V R    Q +S+FDV  G+++ 
Sbjct: 197  SIFVAVFLVIVVSALSN-FRQERQFDKLSKISNNIKVEVLRDSRRQHISIFDVVVGDVVF 256

Query: 238  LKKGDRVPADGLLIKGKNLILDEVI------NSHIDPHRNPFLFSGSVVEYGEGEMIAVS 297
            LK GD++PADGL ++G +L +DE        +  +D   NPFLFSG+ +  G  +M+ VS
Sbjct: 257  LKIGDQIPADGLFLEGHSLQVDESSMTGESDHLEVDHKDNPFLFSGTKIVDGFAQMLVVS 316

Query: 298  IDHDTAFRKGLLDVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLLR-EKH 357
            +   T + + +  +    S+    Q R++     I K  L V+ ++L+V+LVR       
Sbjct: 317  VGMSTTWGQTMSSINQDSSERTPLQVRLDTLTSTIGKIGLTVAALVLVVLLVRYFTGNTE 376

Query: 358  KHGDYYNDKPETKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAIA 417
            K G    +  +T     V+ V +I               VA  +  +V+ I  G+PLA+ 
Sbjct: 377  KEGKREYNGSKTPVDTVVNSVVRI---------------VAAAVTIVVVAIPEGLPLAVT 436

Query: 418  VSLSFWREKMRRSLKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKINP 477
            ++L++  ++M  S +   R LSAC T+G  + IC D T   + +E+++ +F++G+E I+ 
Sbjct: 437  LTLAYSMKRM-MSDQAMVRKLSACETMGSATVICTDKTGTLTLNEMKVTKFWLGQESIHE 496

Query: 478  GME--FHPDI------HQGFEVVARVLRFDPKT---FVLLRNDLLLNFWGESGLEINKES 537
                   PD+        G      V   D  +   F     +  L  W    L ++ ES
Sbjct: 497  DSTKMISPDVLDLLYQGTGLNTTGSVCVSDSGSTPEFSGSPTEKALLSWTVLNLGMDMES 556

Query: 538  LDQRFDIIE-HKFLSSEKGIGVLVNKSRGDAAANLFHEHFYGDASTLLKLCSNYYDIRGR 597
            + Q+ +++    F S++K  GVLV +     + N  H H+ G A  +L +CS+YY   G 
Sbjct: 557  VKQKHEVLRVETFSSAKKRSGVLVRRK----SDNTVHVHWKGAAEMVLAMCSHYYTSTGS 616

Query: 598  IHDIEN-RKDVFEKVIREMEEKGLRPIAFACK-QTNDHQVFEGELKLLGLMGLKF-SHEQ 657
            +  +++  K   + +I+ M    LR IAFA K  +ND  + E  L L+G++GLK      
Sbjct: 617  VDLMDSTAKSRIQAIIQGMAASSLRCIAFAHKIASNDSVLEEDGLTLMGIVGLKDPCRPG 676

Query: 658  ILLALKDLENIGVRIILTSEDELSVAINKADGLGAQCDPNNKE-----VEGPRFREIMKI 717
            +  A++  +  GV I + + D +  A   A   G   D N+K+     VEG +FR     
Sbjct: 677  VSKAVETCKLAGVTIKMITGDNVFTAKAIAFECGI-LDHNDKDEEDAVVEGVQFR---NY 736

Query: 718  NGREKNELMKSITVMGKATSEDKLLLVRELKAKGETVALLGGLTSGDVPTLMEADIGIVQ 777
               E+ + +  I VM +++  DKLL+V+ L+ KG  VA+ G  T+ D P L EADIG+  
Sbjct: 737  TDEERMQKVDKIRVMARSSPSDKLLMVKCLRLKGHVVAVTGDGTN-DAPALKEADIGLSM 796

Query: 778  ENRSTKVSRLVSDLSC--EDVTSLNHTLKYGRSNYLNIQKFYEVQLTALISGLLITLVCT 837
              + T+V++  SD+    ++  S+   LK+GR  Y NIQKF + QLT  ++ L+I  +  
Sbjct: 797  GIQGTEVAKESSDIVILDDNFASVATVLKWGRCVYNNIQKFIQFQLTVNVAALVINFIAA 856

Query: 838  MVSGKSPITTFHLTWVTLIMCLLGSLMMMMELNDEEV--RNPVGGGNREQSLITRVILKK 897
            + +G+ P+T   L WV LIM  LG+L +  E    E+  R PVG   R ++LIT V+ + 
Sbjct: 857  ISAGEVPLTAVQLLWVNLIMDTLGALALATERPTNELLKRKPVG---RTEALITNVMWRN 916

Query: 898  IVIHVLCQALVFLLLEYLGQKIMPHMEEDVRHTMIFNTFILCQISNLLGAITVGLVTNEV 957
            +++  L Q  V L+L++ G  I   + ++V+ T+IFNTF+LCQ+ N   A  +     E 
Sbjct: 917  LLVQSLYQIAVLLILQFKGMSIF-SVRKEVKDTLIFNTFVLCQVFNEFNAREM-----EK 976

Query: 958  VDLQVVLHILWVMISVVGV-LAVQVMVIEFHGTIVNAVKLSAVQWTICFLFALALGWASN 989
             ++   LH   + I ++ + + +QV+++EF     + V+L+  QW  C   A +L W   
Sbjct: 977  KNVFKGLHRNRLFIGIIAITIVLQVIMVEFLKKFADTVRLNGWQWGTCIALA-SLSWPIG 1004

BLAST of HG10005493 vs. ExPASy Swiss-Prot
Match: Q9LIK7 (Putative calcium-transporting ATPase 13, plasma membrane-type OS=Arabidopsis thaliana OX=3702 GN=ACA13 PE=3 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 1.0e-97
Identity = 303/970 (31.24%), Postives = 500/970 (51.55%), Query Frame = 0

Query: 50  PQPLLHTVINVIPSESERNNK--KQRLKLLVKDKDLEALHNFGGVQEAVSFLRSESEPPI 109
           P+ L +T I++     + + K   + L  LVK+K+ E L + GG    VS L+S +   I
Sbjct: 62  PRSLSYTAIDLDHHHGDDHFKIDTETLNDLVKNKNQEKLESLGGPNGLVSALKSNTRLGI 121

Query: 110 DVVGDLAQTVHGLGFWGSLLLF---VKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQ 169
           +  GD  Q       +GS        KG +  +  +    TIL+L+  A LS   G  E 
Sbjct: 122 NEEGDEIQRRRST--FGSNTYTRQPSKGLFHFVVEAFKDLTILILLGCATLSLGFGIKEH 181

Query: 170 GLKHGWHDGVGILLAVFLLVFVPSISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSV 229
           GLK GW+DG  I +AVFL+V V ++S+ +R+  +  +L K+ +N+++ V R+   Q +S+
Sbjct: 182 GLKEGWYDGGSIFVAVFLVVAVSAVSN-FRQNRQFDKLSKVSSNIKIDVVRNGRRQEISI 241

Query: 230 FDVKEGEIIHLKKGDRVPADGLLIKGKNLILDEVINSHIDPH------RNPFLFSGSVVE 289
           FD+  G+I+ L  GD+VPADG+ ++G  L +DE   +    H       N FLFSG+ + 
Sbjct: 242 FDIVVGDIVCLNIGDQVPADGVFVEGHLLHVDESSMTGESDHVEVSLTGNTFLFSGTKIA 301

Query: 290 YGEGEMIAVSIDHDTAFRKGLLDVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVV 349
            G G+M   S+  +TA+ + +  +    +++   QSR++K    I K  L+V+ ++LLV+
Sbjct: 302 DGFGKMAVTSVGMNTAWGQMMSHISRDTNEQTPLQSRLDKLTSSIGKVGLLVAFLVLLVL 361

Query: 350 LVRLL--REKHKHGDYYNDKPETKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVI 409
           L+R      K + G+   +   TK    V+ V K+               VA  +  +V+
Sbjct: 362 LIRYFTGTTKDESGNREYNGKTTKSDEIVNAVVKM---------------VAAAVTIIVV 421

Query: 410 GIQHGMPLAIAVSLSFWREKMRRSLKVNC--RNLSACGTLGLVSAICIDITAEFSFHEVE 469
            I  G+PLA+ ++L++    M+R +K N   R LSAC T+G  + IC D T   + ++++
Sbjct: 422 AIPEGLPLAVTLTLAY---SMKRMMKDNAMVRKLSACETMGSATVICTDKTGTLTLNQMK 481

Query: 470 IREFFVGEEK---INPGMEFHPDIHQGFEVVARVLRFDPKT-----FVLLRNDLLLNFWG 529
           + +F+ G E     +         HQG  +      F  K      F     +  +  W 
Sbjct: 482 VTDFWFGLESGKASSVSQRVVELFHQGVAMNTTGSVFKAKAGTEYEFSGSPTEKAILSWA 541

Query: 530 ESGLEINKESLDQRFDIIE-HKFLSSEKGIGVLVNKSRGDAAANLFHEHFYGDASTLLKL 589
              LE+  E + +  D++    F S +K  GVL+ K   +   N+   H+ G A  +L +
Sbjct: 542 VEELEMGMEKVIEEHDVVHVEGFNSEKKRSGVLMKKKGVNTENNVV--HWKGAAEKILAM 601

Query: 590 CSNYYDIRGRIHDI-ENRKDVFEKVIREMEEKGLRPIAFACKQTND--HQVFEGELKLLG 649
           CS + D  G + ++ E+ K  FEK+I+ M  K LR IAFA  + N+   ++ E +L LLG
Sbjct: 602 CSTFCDGSGVVREMKEDDKIQFEKIIQSMAAKSLRCIAFAYSEDNEDNKKLKEEKLSLLG 661

Query: 650 LMGLKF-SHEQILLALKDLENIGVRIILTSEDEL--SVAINKADGLGAQCDPNNKE--VE 709
           ++G+K      +  A++D +  GV I + + D +  + AI    G+    D  N E  +E
Sbjct: 662 IIGIKDPCRPGVKKAVEDCQFAGVNIKMITGDNIFTARAIAVECGILTPEDEMNSEAVLE 721

Query: 710 GPRFREIMKINGREKNELMKSITVMGKATSEDKLLLVRELKAKGETVALLGGLTSGDVPT 769
           G +FR   +    E+ E ++ I VM +++  DKLL+V+ LK  G  VA+ G  T+ D P 
Sbjct: 722 GEKFRNYTQ---EERLEKVERIKVMARSSPFDKLLMVKCLKELGHVVAVTGDGTN-DAPA 781

Query: 770 LMEADIGIVQENRSTKVSRLVSDLSC--EDVTSLNHTLKYGRSNYLNIQKFYEVQLTALI 829
           L EADIG+    + T+V++  SD+    ++  S+   LK+GR  Y NIQKF + QLT  +
Sbjct: 782 LKEADIGLSMGIQGTEVAKESSDIVILDDNFASVATVLKWGRCVYNNIQKFIQFQLTVNV 841

Query: 830 SGLLITLVCTMVSGKSPITTFHLTWVTLIMCLLGSLMMMME--LNDEEVRNPVGGGNREQ 889
           + L+I  V  + +G  P+T   L WV LIM  LG+L +  E   ND   + P+G   R  
Sbjct: 842 AALVINFVAAVSAGDVPLTAVQLLWVNLIMDTLGALALATEKPTNDLMKKKPIG---RVA 901

Query: 890 SLITRVILKKIVIHVLCQALVFLLLEYLGQKIMPHMEEDVRHTMIFNTFILCQISNLLGA 949
            LIT ++ + ++     Q  V L+L++ G+ I  ++ E V++T+IFNTF+LCQ+ N   A
Sbjct: 902 PLITNIMWRNLLAQAFYQISVLLVLQFRGRSIF-NVTEKVKNTLIFNTFVLCQVFNEFNA 961

Query: 950 ITVGLVTNEVVDLQVVLHILWVMISVVGVLAV-QVMVIEFHGTIVNAVKLSAVQWTICFL 980
            ++     E  ++   LH   + I ++ V  V QV+++EF     +  +L+  QW +C  
Sbjct: 962 RSL-----EKKNVFKGLHKNRLFIGIIVVTVVLQVVMVEFLKRFADTERLNLGQWGVCIA 995

BLAST of HG10005493 vs. ExPASy Swiss-Prot
Match: Q7X8B5 (Calcium-transporting ATPase 5, plasma membrane-type OS=Oryza sativa subsp. japonica OX=39947 GN=ACA5 PE=1 SV=2)

HSP 1 Score: 325.1 bits (832), Expect = 2.8e-87
Identity = 283/973 (29.09%), Postives = 468/973 (48.10%), Query Frame = 0

Query: 70   KKQRLKLLVKDKDLEALHNFGGVQEAVSFLRSESEPPIDVVGDLAQTVHGLGFWGSLLLF 129
            K+ +L  L +D +  AL  +GG+      L++++E  I   GD +        +GS    
Sbjct: 122  KEDQLTALTRDHNYSALQQYGGISGVARMLKTDTEKGIS--GDDSDLTARRNAFGSNTYP 181

Query: 130  VK---GFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGLKHGWHDGVGILLAVFLLVFV 189
             K    F   L+++    T+++L++AA +S A+G   +G+K GW+DG  I  AV L+V V
Sbjct: 182  RKKGRSFLAFLWDACKDLTLIILMVAAAVSLALGITTEGIKEGWYDGASIAFAVLLVVVV 241

Query: 190  PSISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFDVKEGEIIHLKKGDRVPADGL 249
             + S  Y++  + + L + K N+++ V R      VS++D+  G+++ LK GD+VPADG+
Sbjct: 242  TATSD-YKQSLQFQNLNEEKQNIKLEVVRGGRRISVSIYDLVAGDVVPLKIGDQVPADGI 301

Query: 250  LIKGKNLILDEVINS------HIDPHRNPFLFSGSVVEYGEGEMIAVSIDHDTAFRKGLL 309
            LI G +L +DE   +      H D  ++PFL SG  V  G G M+  ++  +T +   + 
Sbjct: 302  LISGHSLSVDESSMTGESKIVHKD-QKSPFLMSGCKVADGYGTMLVTAVGINTEWGLLMA 361

Query: 310  DVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLLREKHKHGDYYNDKPE-- 369
             +     +E   Q R+N    FI    L V+L +L+V+L R        G  YN      
Sbjct: 362  SISEDSGEETPLQVRLNGVATFIGMVGLSVALAVLVVLLARYFT-----GHTYNPDGSVQ 421

Query: 370  -TKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAIAVSLSFWREKM 429
              KGK+ V    +         G   + TVA  +  +V+ +  G+PLA+ ++L+F   KM
Sbjct: 422  YVKGKMGVGQTIR---------GIVGIFTVAVTI--VVVAVPEGLPLAVTLTLAFSMRKM 481

Query: 430  RRSLKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKINP---------- 489
             R  K   R LSAC T+G  + IC D T   + +++ + E + G +K++P          
Sbjct: 482  MRD-KALVRRLSACETMGSATTICSDKTGTLTLNQMTVVEAYFGGKKMDPPDNVQVLSAS 541

Query: 490  --------------GMEFHPDIHQGFEVVARVLRFDPKTFVLLRNDLLLNFWG-ESGLEI 549
                          G  F P+  Q  EV        P    +L        WG + G+  
Sbjct: 542  ISSLIVEGIAQNTSGSIFEPENGQDPEVTG-----SPTEKAILS-------WGLKLGMRF 601

Query: 550  NKESLDQRFDIIEHKFLSSEKGIGVLVNKSRGDAAANLFHEHFYGDASTLLKLCSNYYDI 609
            N ++  +   +    F S +K  GV V+    ++     H H+ G A  +L  C ++   
Sbjct: 602  N-DTRTKSSILHVFPFNSEKKRGGVAVHLGGSESEV---HIHWKGAAEIILDSCKSWLAA 661

Query: 610  RGRIHDIENRK-DVFEKVIREMEEKGLRPIAFACK-----------QTNDHQVFEGELKL 669
             G  H +   K   F+K I +M    LR +AFA +           +  D  + E +L +
Sbjct: 662  DGSKHSMTPEKISEFKKFIEDMAASSLRCVAFAYRTYEMVDVPSEDRRADWILPEDDLIM 721

Query: 670  LGLMGLKFSHEQILLALKDLENI----GVRIILTSEDELSVAINKADGLGAQCDPNNKE- 729
            LG++G+K   +     +KD   +    G+++ + + D L  A   A   G   DPN  E 
Sbjct: 722  LGIVGIK---DPCRPGVKDSVRLCAAAGIKVRMVTGDNLQTARAIALECGILSDPNVSEP 781

Query: 730  --VEGPRFREIMKINGREKNELMKSITVMGKATSEDKLLLVRELKAKGETVALLGGLTSG 789
              +EG  FR +  +   E+ E  + I+VMG+++  DKLLLV+ L+ +G  VA+ G  T+ 
Sbjct: 782  VIIEGKAFRALSDL---EREEAAEKISVMGRSSPNDKLLLVKALRKRGHVVAVTGDGTN- 841

Query: 790  DVPTLMEADIGIVQENRSTKVSRLVSDLSC--EDVTSLNHTLKYGRSNYLNIQKFYEVQL 849
            D P L EADIG+    + T+V++  SD+    ++  S+   +++GRS Y NIQKF + QL
Sbjct: 842  DAPALHEADIGLSMGIQGTEVAKESSDIIILDDNFASVVRVVRWGRSVYANIQKFIQFQL 901

Query: 850  TALISGLLITLVCTMVSGKSPITTFHLTWVTLIMCLLGSLMMMMELNDEEV--RNPVGGG 909
            T  ++ L+I +V  + SG  P+    L WV LIM  LG+L +  E   + +  R PVG  
Sbjct: 902  TVNVAALIINVVAAVSSGNVPLNAVQLLWVNLIMDTLGALALATEPPTDHLMQRPPVG-- 961

Query: 910  NREQSLITRVILKKIVIHVLCQALVFLLLEYLGQKIMPHMEED------VRHTMIFNTFI 969
             R + LIT V+ + ++I  L Q +V L L + G  ++    ++      V++T IFNTF+
Sbjct: 962  -RREPLITNVMWRNLIIMALFQVIVLLTLNFRGTSLLQLKNDNQAHADKVKNTFIFNTFV 1021

BLAST of HG10005493 vs. ExPASy Swiss-Prot
Match: Q9LU41 (Calcium-transporting ATPase 9, plasma membrane-type OS=Arabidopsis thaliana OX=3702 GN=ACA9 PE=2 SV=2)

HSP 1 Score: 319.7 bits (818), Expect = 1.2e-85
Identity = 286/975 (29.33%), Postives = 469/975 (48.10%), Query Frame = 0

Query: 72   QRLKLLVKDKDLEALHNFGGVQEAVSFLRSESEPPIDVVGDLAQTVHGLGFWGSLLL--- 131
            ++L  + +++++  L  +GGV+     L+S  E  I+   D  + +     +GS      
Sbjct: 133  EKLVSMTRNQNMSNLQQYGGVKGVAEKLKSNMEQGIN--EDEKEVIDRKNAFGSNTYPKK 192

Query: 132  FVKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGLKHGWHDGVGILLAVFLLVFVPS 191
              K F++ L+ +    T+++L+IAA  S A+G   +GLK GW DG  I  AV L++ V +
Sbjct: 193  KGKNFFMFLWEAWQDLTLIILIIAAVTSLALGIKTEGLKEGWLDGGSIAFAVLLVIVVTA 252

Query: 192  ISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFDVKEGEIIHLKKGDRVPADGLLI 251
            +S  YR+  + + L   K N+++ V R     ++S++DV  G++I L+ GD+VPADG+LI
Sbjct: 253  VSD-YRQSLQFQNLNDEKRNIQLEVMRGGRTVKISIYDVVVGDVIPLRIGDQVPADGVLI 312

Query: 252  KGKNLILDEVINS------HIDPHRNPFLFSGSVVEYGEGEMIAVSIDHDTAFRKGLLDV 311
             G +L +DE   +      H D  ++PFL SG  V  G G M+   +  +T +   +  +
Sbjct: 313  SGHSLAIDESSMTGESKIVHKD-QKSPFLMSGCKVADGVGNMLVTGVGINTEWGLLMASI 372

Query: 312  IVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLL--REKHKHGDYYNDKPETK 371
                 +E   Q R+N    FI    L V+L++L+ +LVR      +  +G     K  T 
Sbjct: 373  SEDTGEETPLQVRLNGLATFIGIVGLSVALVVLVALLVRYFTGTTQDTNGATQFIKGTTS 432

Query: 372  GKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAIAVSLSFWREKMRRS 431
                VD   KIF             T+A  +  +V+ +  G+PLA+ ++L++   KM   
Sbjct: 433  ISDIVDDCVKIF-------------TIAVTI--VVVAVPEGLPLAVTLTLAYSMRKMMAD 492

Query: 432  LKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKINPGMEFHPDIHQGFE 491
             K   R LSAC T+G  + IC D T   + +++ + E + G  K+              +
Sbjct: 493  -KALVRRLSACETMGSATTICSDKTGTLTLNQMTVVETYAGGSKM--------------D 552

Query: 492  VVARVLRFDPKTFVLLRNDLLLNFWGE------------SGLEINKE------SLDQRFD 551
            V        PK   L+   +  N  G             SG    K        L  +FD
Sbjct: 553  VADNPSGLHPKLVALISEGVAQNTTGNIFHPKDGGEVEISGSPTEKAILSWAYKLGMKFD 612

Query: 552  IIE------HKF-LSSEK---GIGVLVNKSRGDAAANLFHEHFYGDASTLLKLCSNYYDI 611
             I       H F  +SEK   G+ VL    RGD+   +   H+ G A  +L  C+ Y D 
Sbjct: 613  TIRSESAIIHAFPFNSEKKRGGVAVL----RGDSEVFI---HWKGAAEIVLACCTQYMDS 672

Query: 612  RGRIHDIENRKDVFEKVIREMEEKGLRPIAFACKQTNDHQV------------FEGELKL 671
             G +  IE++K+ F   I  M +  LR +A AC+    +QV             E EL L
Sbjct: 673  NGTLQSIESQKEFFRVAIDSMAKNSLRCVAIACRTQELNQVPKEQEDLDKWALPEDELIL 732

Query: 672  LGLMGLKF-SHEQILLALKDLENIGVRIILTSEDELSVAINKADGLGAQCDPNNKE---- 731
            L ++G+K      +  A++   + GV++ + + D L  A  KA  L      ++ E    
Sbjct: 733  LAIVGIKDPCRPGVREAVRICTSAGVKVRMVTGDNLQTA--KAIALECGILSSDTEAVEP 792

Query: 732  --VEGPRFREIMKINGREKNELMKSITVMGKATSEDKLLLVRELKAKGETVALLGGLTSG 791
              +EG  FRE+ +   +E+ ++ K ITVMG+++  DKLLLV+ L+  G+ VA+ G  T+ 
Sbjct: 793  TIIEGKVFRELSE---KEREQVAKKITVMGRSSPNDKLLLVQALRKNGDVVAVTGDGTN- 852

Query: 792  DVPTLMEADIGIVQENRSTKVSRLVSDLSC--EDVTSLNHTLKYGRSNYLNIQKFYEVQL 851
            D P L EADIG+      T+V++  SD+    ++  S+   +++GRS Y NIQKF + QL
Sbjct: 853  DAPALHEADIGLSMGISGTEVAKESSDIIILDDNFASVVKVVRWGRSVYANIQKFIQFQL 912

Query: 852  TALISGLLITLVCTMVSGKSPITTFHLTWVTLIMCLLGSLMMMMELNDEEV--RNPVGGG 911
            T  ++ L+I +V  M SG  P+    L WV LIM  LG+L +  E   + +  R PVG  
Sbjct: 913  TVNVAALIINVVAAMSSGDVPLKAVQLLWVNLIMDTLGALALATEPPTDHLMHRTPVG-- 972

Query: 912  NREQSLITRVILKKIVIHVLCQALVFLLLEYLGQKIMPHMEE------DVRHTMIFNTFI 971
             R + LIT ++ + +++    Q  V L+L + G  I+    E      +V++TMIFN F+
Sbjct: 973  -RREPLITNIMWRNLLVQSFYQVAVLLVLNFAGLSILGLNHENHAHAVEVKNTMIFNAFV 1032

Query: 972  LCQISNLLGAITVGLVTNEVVDLQVVLHILW--VMISVVGV-LAVQVMVIEFHGTIVNAV 976
            +CQI N   A        +  ++ V   +    + +++VGV   +Q++++ F G   + V
Sbjct: 1033 MCQIFNEFNA-------RKPDEMNVFRGVNKNPLFVAIVGVTFILQIIIVTFLGKFAHTV 1050

BLAST of HG10005493 vs. ExPASy Swiss-Prot
Match: Q9LF79 (Calcium-transporting ATPase 8, plasma membrane-type OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=1 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 2.9e-84
Identity = 276/958 (28.81%), Postives = 465/958 (48.54%), Query Frame = 0

Query: 72   QRLKLLVKDKDLEALHNFGGVQEAVSFLRSESEPPIDVVGD---LAQTVHGLGFWGSLLL 131
            ++L ++ KD +  AL  +GG Q   + L++  E  I    D     +T++G   +     
Sbjct: 119  EQLVIMSKDHNSGALEQYGGTQGLANLLKTNPEKGISGDDDDLLKRKTIYGSNTYPRKK- 178

Query: 132  FVKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGLKHGWHDGVGILLAVFLLVFVPS 191
              KGF   L+++ +  T+++L++AA  S A+G   +G+K GW+DG  I  AV L++ V +
Sbjct: 179  -GKGFLRFLWDACHDLTLIILMVAAVASLALGIKTEGIKEGWYDGGSIAFAVILVIVVTA 238

Query: 192  ISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFDVKEGEIIHLKKGDRVPADGLLI 251
            +S  Y++  + + L   K N+ + V R      +S++D+  G++I L  G++VPADG+LI
Sbjct: 239  VSD-YKQSLQFQNLNDEKRNIHLEVLRGGRRVEISIYDIVVGDVIPLNIGNQVPADGVLI 298

Query: 252  KGKNLILDE--------VINSHIDPHRNPFLFSGSVVEYGEGEMIAVSIDHDTAFRKGLL 311
             G +L LDE        ++N   D +++PFL SG  V  G G M+   +  +T +   + 
Sbjct: 299  SGHSLALDESSMTGESKIVNK--DANKDPFLMSGCKVADGNGSMLVTGVGVNTEWGLLMA 358

Query: 312  DVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLL--REKHKHGDYYNDKPE 371
             +     +E   Q R+N    FI    L V+  +L+++L R      K  +G     K +
Sbjct: 359  SISEDNGEETPLQVRLNGVATFIGSIGLAVAAAVLVILLTRYFTGHTKDNNGGPQFVKGK 418

Query: 372  TKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAIAVSLSFWREKMR 431
            TK    +D V K+              TVA  +  +V+ +  G+PLA+ ++L++   KM 
Sbjct: 419  TKVGHVIDDVVKVL-------------TVAVTI--VVVAVPEGLPLAVTLTLAYSMRKMM 478

Query: 432  RSLKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKINPGM---EFHPDI 491
               K   R LSAC T+G  + IC D T   + +++ + E + G +K +           +
Sbjct: 479  AD-KALVRRLSACETMGSATTICSDKTGTLTLNQMTVVESYAGGKKTDTEQLPATITSLV 538

Query: 492  HQGFEVVARVLRFDPK-----TFVLLRNDLLLNFWGESGLEINKESLDQRFDIIE-HKFL 551
             +G         F P+      +     +  +  WG   L +N E+   +  I+    F 
Sbjct: 539  VEGISQNTTGSIFVPEGGGDLEYSGSPTEKAILGWGVK-LGMNFETARSQSSILHAFPFN 598

Query: 552  SSEKGIGVLVNKSRGDAAANLFHEHFYGDASTLLKLCSNYYDIRGRIHDI-ENRKDVFEK 611
            S +K  GV V  + G+      H H+ G +  +L  C +Y D  G +  + +++   F+ 
Sbjct: 599  SEKKRGGVAVKTADGEV-----HVHWKGASEIVLASCRSYIDEDGNVAPMTDDKASFFKN 658

Query: 612  VIREMEEKGLRPIAFACKQTNDHQVFEGE-----------LKLLGLMGLKFSHEQILLAL 671
             I +M  + LR +A A +     +V  GE           L LL ++G+K   +     +
Sbjct: 659  GINDMAGRTLRCVALAFRTYEAEKVPTGEELSKWVLPEDDLILLAIVGIK---DPCRPGV 718

Query: 672  KD----LENIGVRIILTSEDELSVAINKADGLG-AQCDPNNKE---VEGPRFREIMKING 731
            KD     +N GV++ + + D +  A   A   G    D +  E   +EG  FRE   +  
Sbjct: 719  KDSVVLCQNAGVKVRMVTGDNVQTARAIALECGILSSDADLSEPTLIEGKSFRE---MTD 778

Query: 732  REKNELMKSITVMGKATSEDKLLLVRELKAKGETVALLGGLTSGDVPTLMEADIGIVQEN 791
             E++++   I+VMG+++  DKLLLV+ L+ +G  VA+ G  T+ D P L EADIG+    
Sbjct: 779  AERDKISDKISVMGRSSPNDKLLLVQSLRRQGHVVAVTGDGTN-DAPALHEADIGLAMGI 838

Query: 792  RSTKVSRLVSDLSC--EDVTSLNHTLKYGRSNYLNIQKFYEVQLTALISGLLITLVCTMV 851
              T+V++  SD+    ++  S+   +++GRS Y NIQKF + QLT  ++ L+I +V  + 
Sbjct: 839  AGTEVAKESSDIIILDDNFASVVKVVRWGRSVYANIQKFIQFQLTVNVAALVINVVAAIS 898

Query: 852  SGKSPITTFHLTWVTLIMCLLGSLMMMMELNDEEV--RNPVGGGNREQSLITRVILKKIV 911
            SG  P+T   L WV LIM  LG+L +  E   + +  R PVG   R++ LIT ++ + ++
Sbjct: 899  SGDVPLTAVQLLWVNLIMDTLGALALATEPPTDHLMGRPPVG---RKEPLITNIMWRNLL 958

Query: 912  IHVLCQALVFLLLEYLGQKIMPHMEE------DVRHTMIFNTFILCQISNLLGA------ 971
            I  + Q  V L L + G  I+    E       V++T+IFN F+LCQ  N   A      
Sbjct: 959  IQAIYQVSVLLTLNFRGISILGLEHEVHEHATRVKNTIIFNAFVLCQAFNEFNARKPDEK 1018

BLAST of HG10005493 vs. ExPASy TrEMBL
Match: A0A0A0K6H2 (Cation_ATPase_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G033290 PE=4 SV=1)

HSP 1 Score: 1293.5 bits (3346), Expect = 0.0e+00
Identity = 674/888 (75.90%), Postives = 753/888 (84.80%), Query Frame = 0

Query: 107 IDVVGDLAQTVHGLGFWGSLLLFVKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGL 166
           ID +GDLAQT    GFW SLLLF+K FW  LYNS NS TIL+LV AA  S AIGS+EQGL
Sbjct: 5   IDAIGDLAQT----GFWDSLLLFLKAFWSSLYNSFNSSTILLLVFAAGFSLAIGSIEQGL 64

Query: 167 KHGWHDGVGILLAVFLLVFVPSISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFD 226
           KHGWHD VGILLAVFLL+F  S+    +KKAEEK+ LKIKNN +VTVKRHE LQ +SVFD
Sbjct: 65  KHGWHDCVGILLAVFLLLFFSSVLGFCKKKAEEKKRLKIKNNFKVTVKRHEELQEISVFD 124

Query: 227 VKEGEIIHLKKGDRVPADGLLIKGKNLILDEVINSHIDPHRNPFLFSGSVVEYGEGEMIA 286
           VKEGEIIHLKKGDRV ADGLLIKG NLILDE INSHIDP RNPFL SGSVVEYGEGEM+A
Sbjct: 125 VKEGEIIHLKKGDRVLADGLLIKGNNLILDEAINSHIDPRRNPFLLSGSVVEYGEGEMLA 184

Query: 287 VSIDHDTAFRKGLLDVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLLREK 346
           VSID DTAF KGLLDVIV+PSQE LFQSRINKPYEF EKFSL + LM+LLVVL RLL EK
Sbjct: 185 VSIDRDTAFWKGLLDVIVYPSQETLFQSRINKPYEFFEKFSLGLYLMVLLVVLTRLLCEK 244

Query: 347 HKHGDYYNDKPETKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAI 406
           H+HG+YYNDKPETKGKLTV F+   F RM   FGK+RVS+VAT + TMV+GIQHGMPLAI
Sbjct: 245 HEHGNYYNDKPETKGKLTVAFIGNAFQRMSFVFGKYRVSSVATVVFTMVVGIQHGMPLAI 304

Query: 407 AVSLSFWREKMRRSLKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKIN 466
             SLSFWREKMRRS KVNC+NLSACGTLGLVS ICID+ AE SF +VE+ EF+VGEEKIN
Sbjct: 305 TFSLSFWREKMRRSHKVNCQNLSACGTLGLVSVICIDVAAELSFCDVEVDEFYVGEEKIN 364

Query: 467 PGMEFHPDIHQGFEVVARVLRFDPKTFVLLRNDLLLNFWGESGLEINKE-SLDQRFDIIE 526
           PGMEFH D+HQGFE  +RVLR DPKT  L  ++ LL+FW  SGL+IN E  LDQ FDII+
Sbjct: 365 PGMEFHLDVHQGFEAASRVLRIDPKTTFL--SEYLLDFWKNSGLKINNEPDLDQMFDIID 424

Query: 527 HKFLSSEKGIGVLVNKSR-GDAAANLFHEHFYGDASTLLKLCSNYYDIRGRIHDIENRKD 586
           HKFLSSE+ +GVLVNK+R GD  +NL HEHFYGDAST+L +CSNYYDI GR+HDIENR D
Sbjct: 425 HKFLSSEESMGVLVNKTRGGDTVSNLIHEHFYGDASTILNMCSNYYDIGGRVHDIENRND 484

Query: 587 VFEKVIREMEEKGLRPIAFACKQTNDHQVFEGELKLLGLMGLKFSHEQILLALKDLENIG 646
           V +  I+EMEEKGLRPIAFACKQ ND   FEGELKLLG MGLK SHE+I  ALKDL+NIG
Sbjct: 485 VLQTKIKEMEEKGLRPIAFACKQKNDQTGFEGELKLLGYMGLKISHEKIQHALKDLQNIG 544

Query: 647 VRIILTSEDELSVAINKADGLGAQCDPNNKEVEG-PRFREI-MKINGREKNELMKSITVM 706
           +RIILTS+D +SV I  A  LG +CDPNN++ EG  R REI MK N REKNELMKSIT M
Sbjct: 545 IRIILTSKDNVSVIIKMAGDLGTRCDPNNRQTEGDQRLREILMKNNEREKNELMKSITFM 604

Query: 707 GKATSEDKLLLVRELKAKGETVALLGGLTSGDVPTLMEADIGIVQENRSTKVSRLVSDLS 766
           GKATS+DKL+LV+ELKAKGETVA +GGLTSGDVPTL+EADIGI+QENRSTK  +LVSDL 
Sbjct: 605 GKATSDDKLVLVKELKAKGETVAFIGGLTSGDVPTLIEADIGIIQENRSTKECKLVSDLR 664

Query: 767 CEDVTSLNHTLKYGRSNYLNIQKFYEVQLTALISGLLITLVCTMVSGKSPITTFHLTWVT 826
            EDVTSLNHTLKYGRSNYLNI+KFY++QLTALISGLLITL+CTMVSGKSPIT+FHLTWVT
Sbjct: 665 YEDVTSLNHTLKYGRSNYLNIKKFYQLQLTALISGLLITLICTMVSGKSPITSFHLTWVT 724

Query: 827 LIMCLLGSLMMMMELNDEEVRNPVGGGNREQSLITRVILKKIVIHVLCQALVFLLLEYLG 886
           LI CLLG LMM+MELNDEEV+N VGG +R Q+LITR I+KKIVIHVLCQA VFL++EYLG
Sbjct: 725 LITCLLGGLMMVMELNDEEVQNVVGGSDRNQALITRDIVKKIVIHVLCQASVFLIIEYLG 784

Query: 887 QKIMPHMEEDVRHTMIFNTFILCQISNLLGAITVGLVTNEVVDLQVVLHILWVMISVVGV 946
            KI+P M+EDVR TMIFNT+ILCQI+NLLGAI+VGLVTN     QV + ILWV+I VVGV
Sbjct: 785 HKIVPQMKEDVRDTMIFNTYILCQIANLLGAISVGLVTNRAAVFQVAVQILWVLIFVVGV 844

Query: 947 LAVQVMVIEFHGTIVNAVKLSAVQWTICFLFALALGWASNIFFHFLLH 991
           LAVQV+VIE HGTIVN VKLSA+QW ICFLFALALGWAS IF HF +H
Sbjct: 845 LAVQVVVIELHGTIVNGVKLSALQWIICFLFALALGWASYIFLHFAIH 886

BLAST of HG10005493 vs. ExPASy TrEMBL
Match: A0A1S3CG68 (calcium-transporting ATPase 12, plasma membrane-type-like OS=Cucumis melo OX=3656 GN=LOC103500558 PE=4 SV=1)

HSP 1 Score: 1275.0 bits (3298), Expect = 0.0e+00
Identity = 667/890 (74.94%), Postives = 746/890 (83.82%), Query Frame = 0

Query: 104 EPP-IDVVGDLAQTVHGLGFWGSLLLFVKGFWLCLYNSLNSCTILVLVIAADLSFAIGSL 163
           EPP  D +GDLAQTV GLG  GSLLLFVKGFW CLYNSLNSCTIL+LVIAA  S AIGS+
Sbjct: 3   EPPDNDAIGDLAQTVGGLGLRGSLLLFVKGFWSCLYNSLNSCTILLLVIAAGFSLAIGSI 62

Query: 164 EQGLKHGWHDGVGILLAVFLLVFVPSISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRV 223
           EQGLK GWHD VGILLA+FLLVF  S+ S  +KKAEEK+ LKIKNNL+VTVKRHE L  +
Sbjct: 63  EQGLKDGWHDCVGILLAIFLLVFFSSVLSFCKKKAEEKKRLKIKNNLKVTVKRHEELVEI 122

Query: 224 SVFDVKEGEIIHLKKGDRVPADGLLIKGKNLILDEVINSHIDPHRNPFLFSGSVVEYGEG 283
           SVFDVKEGEIIHLKKGD V ADGLL KGKN+ILDE INSHIDPHRNPFLFSGSVVEYGEG
Sbjct: 123 SVFDVKEGEIIHLKKGDHVLADGLLTKGKNMILDEAINSHIDPHRNPFLFSGSVVEYGEG 182

Query: 284 EMIAVSIDHDTAFRKGLLDVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRL 343
           EMIAVSI+ DTAF KGLLDVIVHPSQE LFQSRINKPYEFIEKFSLV+ LM+LLVVL+RL
Sbjct: 183 EMIAVSINRDTAFWKGLLDVIVHPSQETLFQSRINKPYEFIEKFSLVMYLMVLLVVLIRL 242

Query: 344 LREKHKHGDYYNDKPETKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGM 403
           L +K++HG+YYNDKPE KGKLTV FV   F RM   F K+RVS+VAT +L +V+GIQHGM
Sbjct: 243 LCKKNEHGNYYNDKPENKGKLTVAFVGNAFERMSFVFAKYRVSSVATAVLIIVVGIQHGM 302

Query: 404 PLAIAVSLSFWREKMRRSLKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGE 463
           PLAI VSL FWREKMRRS KVNC+NLSACGTLGLVS IC+DITAE SF++VE+ EF+VGE
Sbjct: 303 PLAITVSLFFWREKMRRSHKVNCQNLSACGTLGLVSVICVDITAELSFYDVEVDEFYVGE 362

Query: 464 EKINPGMEFHPDIHQGFEVVARVLRFDPKTFVLLRNDLLLNFWGESGLEINKE-SLDQRF 523
           EKINPGMEFH DIH+G E  + VLRFDPKT V L N  LL+FW  SGL+IN E  LD+ F
Sbjct: 363 EKINPGMEFHLDIHRGIEAASGVLRFDPKTTVRLENK-LLDFWENSGLKINNEPDLDEMF 422

Query: 524 DIIEHKFLSSEKGIGVLVNKSR-GDAAANLFHEHFYGDASTLLKLCSNYYDIRGRIHDIE 583
           DII+HKFLSSEKGIGVLV K+R G+  ANL HE+FYGDAST+L +CSNYYD+ GRIHDIE
Sbjct: 423 DIIDHKFLSSEKGIGVLVYKTRGGETGANLIHENFYGDASTILNMCSNYYDMGGRIHDIE 482

Query: 584 NRKDVFEKVIREMEEKGLRPIAFACKQTNDHQVFEGELKLLGLMGLKFSHEQILLALKDL 643
           NRKDV EK IREMEEKGLRPIAFA   TND  VFEGELKLLG MGLK S +++L ALKDL
Sbjct: 483 NRKDVLEKKIREMEEKGLRPIAFAGNHTNDQTVFEGELKLLGYMGLKISQQEVLRALKDL 542

Query: 644 ENIGVRIILTSEDELSVAINKADGLGAQCDPNNKEVEGPRFREIMKINGREKNELMKSIT 703
           +N G+RIILTSED+LS  IN AD LG +CDPNNKE+EG RFREIMKI+G +KNELMKSIT
Sbjct: 543 KNNGIRIILTSEDKLSTIINMADDLGIRCDPNNKEIEGERFREIMKIDGMDKNELMKSIT 602

Query: 704 VMGKATSEDKLLLVRELKAKGETVALLGGLTSGDVPTLMEADIGIVQENRSTKVSRLVSD 763
           +MGKATS+DKL+LV+ELKA  E VA +GGLTS D+PTL+EADIGI+QENRSTK  +LVSD
Sbjct: 603 LMGKATSDDKLVLVKELKANREVVAFIGGLTSEDLPTLIEADIGIIQENRSTKECKLVSD 662

Query: 764 LSCEDVTSLNHTLKYGRSNYLNIQKFYEVQLTALISGLLITLVCTMVSGKSPITTFHLTW 823
           LS EDVTSLNHTLKYGR NYLNIQKFY+VQL A ISGLLITL+CT+VSGKSPIT+FHLTW
Sbjct: 663 LSYEDVTSLNHTLKYGRINYLNIQKFYQVQLAASISGLLITLICTIVSGKSPITSFHLTW 722

Query: 824 VTLIMCLLGSLMMMMELNDEEVRNPVGGGNREQSLITRVILKKIVIHVLCQALVFLLLEY 883
           +TLIMCLLGSLMM+ME +DEEVRN VGGG+R Q+LITRV LKKIVIH LCQA VFL+LEY
Sbjct: 723 MTLIMCLLGSLMMVMESSDEEVRNLVGGGDRNQTLITRVTLKKIVIHALCQASVFLILEY 782

Query: 884 LGQKIMPHMEEDVRHTMIFNTFILCQISNLLGAITVGLVTNEVVDLQVVLHILWVMISVV 943
           +G KI+P M+EDV+ TMIFNTFILCQ++NLLGAITVGL                      
Sbjct: 783 VGHKIVPQMKEDVKETMIFNTFILCQMANLLGAITVGL---------------------- 842

Query: 944 GVLAVQVMVIEFHGTIVNAVKLSAVQWTICFLFALALGWASNIFFHFLLH 991
                 V+VIE  GTIVN VKLSA+QW ICFLFA ALGWAS IFFHF+LH
Sbjct: 843 ------VVVIEVDGTIVNGVKLSALQWIICFLFASALGWASYIFFHFVLH 863

BLAST of HG10005493 vs. ExPASy TrEMBL
Match: A0A5A7UTE0 (Calcium-transporting ATPase 12, plasma membrane-type-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G001200 PE=4 SV=1)

HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 654/885 (73.90%), Postives = 736/885 (83.16%), Query Frame = 0

Query: 108 DVVGDLAQTVHGLGFWGSLLLFVKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGLK 167
           D +GDLA+TV GLG  GSLLLFVKGFW CLYNSLNSCTIL+LVIAA+ S AIGS+EQGLK
Sbjct: 5   DAIGDLAETVGGLGLRGSLLLFVKGFWSCLYNSLNSCTILLLVIAAEFSLAIGSIEQGLK 64

Query: 168 HGWHDGVGILLAVFLLVFVPSISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFDV 227
            GWHD VGILLA+FLLVF  S+ S  +KKAEEK+ LKIKNNL+VTVKRHE L  +SVFDV
Sbjct: 65  DGWHDCVGILLAIFLLVFFSSVLSFCKKKAEEKKRLKIKNNLKVTVKRHEELVEISVFDV 124

Query: 228 KEGEIIHLKKGDRVPADGLLIKGKNLILDEVINSHIDPHRNPFLFSGSVVEYGEGEMIAV 287
           KEGEIIHLKKGD V ADGLL KGKN+ILDE INSHIDPHRNPFLFSGSVVEYGEGEMIAV
Sbjct: 125 KEGEIIHLKKGDHVLADGLLTKGKNMILDEAINSHIDPHRNPFLFSGSVVEYGEGEMIAV 184

Query: 288 SIDHDTAFRKGLLDVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLLREKH 347
           SI+ DTAF KGLLDVIVHPSQE LFQSRINKPYEFIEKFSLV+ LM+LLVVL+RLL +K+
Sbjct: 185 SINRDTAFWKGLLDVIVHPSQETLFQSRINKPYEFIEKFSLVMYLMVLLVVLIRLLCKKN 244

Query: 348 KHGDYYNDKPETKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAIA 407
           +HG+YYNDKPE KGKLTV FV   F RM   FGK+RVS+VAT +L +V+GIQHGMPLAI 
Sbjct: 245 EHGNYYNDKPENKGKLTVAFVGNAFERMSFVFGKYRVSSVATAVLIIVVGIQHGMPLAIT 304

Query: 408 VSLSFWREKMRRSLKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKINP 467
           VSL FWRE      K++   LS                         + EF+VGEEKINP
Sbjct: 305 VSLFFWRE----DEKISQSKLSE-----------------------SVDEFYVGEEKINP 364

Query: 468 GMEFHPDIHQGFEVVARVLRFDPKTFVLLRNDLLLNFWGESGLEINKE-SLDQRFDIIEH 527
           GMEFH DIH+G E  +RVLRFDPKT V L N  LL+FW  SGL+IN E  LD+ FDII+H
Sbjct: 365 GMEFHLDIHRGIEAASRVLRFDPKTTVRLENK-LLDFWENSGLKINNEPDLDEMFDIIDH 424

Query: 528 KFLSSEKGIGVLVNKSR-GDAAANLFHEHFYGDASTLLKLCSNYYDIRGRIHDIENRKDV 587
           KFLSSEKGIGVLV K+R G+  ANL HE+FYGDAST+L +CSNYYD+ GRIHDIENRKDV
Sbjct: 425 KFLSSEKGIGVLVYKTRGGETGANLIHENFYGDASTILNMCSNYYDMGGRIHDIENRKDV 484

Query: 588 FEKVIREMEEKGLRPIAFACKQTNDHQVFEGELKLLGLMGLKFSHEQILLALKDLENIGV 647
            EK IREMEEKGLRPIAFA   TND  VFEGELKLLG MGLK S +++L ALKDL+N G+
Sbjct: 485 LEKKIREMEEKGLRPIAFARNHTNDQTVFEGELKLLGYMGLKISQQEVLRALKDLKNNGI 544

Query: 648 RIILTSEDELSVAINKADGLGAQCDPNNKEVEGPRFREIMKINGREKNELMKSITVMGKA 707
           RIIL SED+LS  IN AD LG +CDPNNKE+EG RFREIMKI+G +KNELMKSIT+MGKA
Sbjct: 545 RIILISEDKLSTIINMADDLGIRCDPNNKEIEGERFREIMKIDGMDKNELMKSITLMGKA 604

Query: 708 TSEDKLLLVRELKAKGETVALLGGLTSGDVPTLMEADIGIVQENRSTKVSRLVSDLSCED 767
           TS+DKL+LV+ELKA  E VA +GGLTS D+PTL+EADIGI+QENRSTK  +LVSDLS ED
Sbjct: 605 TSDDKLVLVKELKANREVVAFIGGLTSEDLPTLIEADIGIIQENRSTKECKLVSDLSYED 664

Query: 768 VTSLNHTLKYGRSNYLNIQKFYEVQLTALISGLLITLVCTMVSGKSPITTFHLTWVTLIM 827
           VTSLNHTLKYGR NYLNIQKFY+VQL A ISGLLITL+CT+VSGKSPIT+FHLTW+TLIM
Sbjct: 665 VTSLNHTLKYGRINYLNIQKFYQVQLAASISGLLITLICTIVSGKSPITSFHLTWMTLIM 724

Query: 828 CLLGSLMMMMELNDEEVRNPVGGGNREQSLITRVILKKIVIHVLCQALVFLLLEYLGQKI 887
           CLLGSLMM+ME +DEEVRN VGGG+R Q+LITRV LKKIVIH LCQA VFL+LEY+G KI
Sbjct: 725 CLLGSLMMVMESSDEEVRNLVGGGDRNQTLITRVTLKKIVIHALCQASVFLILEYVGHKI 784

Query: 888 MPHMEEDVRHTMIFNTFILCQISNLLGAITVGLVTNEVVDLQVVLHILWVMISVVGVLAV 947
           +P M+EDV+ TMIFNTFILCQ++NLLGAITVGLVTN+    QV LHI WV+ISVVGVLA+
Sbjct: 785 VPQMKEDVKETMIFNTFILCQMANLLGAITVGLVTNKAAVFQVALHIFWVLISVVGVLAL 844

Query: 948 QVMVIEFHGTIVNAVKLSAVQWTICFLFALALGWASNIFFHFLLH 991
           QV+VIE  GTIVN VKLSA+QW ICFLFA ALGWAS IFFHF+LH
Sbjct: 845 QVVVIEVDGTIVNGVKLSALQWIICFLFASALGWASYIFFHFVLH 861

BLAST of HG10005493 vs. ExPASy TrEMBL
Match: A0A6J1HUY0 (putative calcium-transporting ATPase 13, plasma membrane-type OS=Cucurbita maxima OX=3661 GN=LOC111468109 PE=4 SV=1)

HSP 1 Score: 1212.2 bits (3135), Expect = 0.0e+00
Identity = 670/982 (68.23%), Postives = 775/982 (78.92%), Query Frame = 0

Query: 1   MFQGTDDDGGAQPLLVVSAVATSYKPAGFKFRQLVLSIRFVLCLNRTRS---PQPLLHTV 60
           MFQ T DD GA+PLLV + V+ S K    +FRQLVLSIRFVL L RT S   P P  +TV
Sbjct: 3   MFQRTADD-GAEPLLVATPVSNSSKLV--RFRQLVLSIRFVLSLKRTSSPPPPSPSQYTV 62

Query: 61  INVIPSESERNNKKQRLKLLVKDKDLEALHNFGGVQEAVSFLRSESEPPIDVVGDLAQTV 120
           I+V   E ER+ +KQRLK +VK+K+L+AL + GGV+EAVSFLRSES   ID     AQT+
Sbjct: 63  IDV-HFEDERDIQKQRLKKIVKEKNLKALKDLGGVEEAVSFLRSESLVQID-----AQTL 122

Query: 121 HGLGFWGSLLLFVKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGLKHGWHDGVGIL 180
            G GF GS LLFVK F   L+NSLNS TIL LVIAA LSFAI SL+QGLKHGWHDG GIL
Sbjct: 123 RGYGFCGSFLLFVKEFCCYLWNSLNSWTILFLVIAAGLSFAIESLQQGLKHGWHDGFGIL 182

Query: 181 LAVFLLVFVPSISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFDVKEGEIIHLKK 240
           LAVFLLVF P I S+YRK+AEEK LLK KN LEVTVKR E+ QRVSV DV+EG+ IHL K
Sbjct: 183 LAVFLLVFFPPILSVYRKRAEEKELLKNKNILEVTVKRDELCQRVSVSDVREGDRIHLNK 242

Query: 241 GDRVPADGLLIKGKNLILDEVINSHIDPHRNPFLFSGSVVEYGEGEMIAVSIDHDTAFRK 300
           GDRVPADGLLIKGKNLILDEVINSHIDP+RNPFLF GSVVE+GEGEMIAVS+ HDTAF +
Sbjct: 243 GDRVPADGLLIKGKNLILDEVINSHIDPNRNPFLFCGSVVEHGEGEMIAVSVGHDTAFGE 302

Query: 301 GLLDVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLLREKHKHGDYYNDKP 360
            LL+++ HPSQE LFQSR+NKPY+F+EKFSL VSL +L+VVL RLL  KH H DYYND+P
Sbjct: 303 VLLELVTHPSQETLFQSRMNKPYQFLEKFSLTVSLTILVVVLARLLC-KH-HDDYYNDRP 362

Query: 361 ETKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAIAVSLSFWREKM 420
           ETKGKLT   V + F RMFL+FG  R S +AT LLTM IG+QHGMP AI VSLSFWREKM
Sbjct: 363 ETKGKLTAGIVVQAFERMFLKFGS-RASFLATVLLTMAIGLQHGMPFAITVSLSFWREKM 422

Query: 421 RRSLKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKINPGMEFHPDIHQ 480
            RSLK NC NLSACGTLGLVSAICID+TAE SFHEVE+ EFFVGE+KIN  MEFH DI Q
Sbjct: 423 -RSLKGNCLNLSACGTLGLVSAICIDVTAELSFHEVELCEFFVGEQKINSDMEFHTDISQ 482

Query: 481 GFEVVARVLRFDPKTFVLLRNDLLLNFWGESGLEINKESLDQRFDIIEHKFLSSEKGIGV 540
             E+ A++L  DPK  V L NDL+     + G      + DQ+FDII+HKFLSSEKGIG 
Sbjct: 483 SLELAAQILCSDPKVSVHLGNDLVHFLKNKLG------APDQKFDIIDHKFLSSEKGIGA 542

Query: 541 LVNK-SRGDAAANLFHEHFYGDASTLLKLCSNYYDIRGRIHDIENRKDVFEKVIREMEEK 600
           LV+K S  D  ANLFH H+YGDAS+++ +CS YYDIRG +HDIEN KDVFE VIREM+++
Sbjct: 543 LVSKRSEMDTEANLFHVHYYGDASSIVSMCSQYYDIRGIVHDIENEKDVFENVIREMKQR 602

Query: 601 GLRPIAFACKQ-TNDHQVFEGELKLLGLMGLKFSHEQILLALKDLENIGVRIILTSEDEL 660
           GLRPIAFACKQ  NDHQ+FEGE KLLG +GLKFSHE+ L AL+DL+NIG RIIL SEDEL
Sbjct: 603 GLRPIAFACKQRRNDHQLFEGESKLLGFIGLKFSHEKTLNALRDLQNIGARIILISEDEL 662

Query: 661 SVAINKADGLGAQCDPNNKEVEGPRFREIMKINGREKNELMKSITVMGKATSEDKLLLVR 720
             AIN  +GLG Q D  N  VEG RF+EIM  +G E++ELMK+I+ MGKATSEDK LL++
Sbjct: 663 FEAINMVNGLGTQWDLKNNVVEGERFKEIMMTDGTERHELMKNISFMGKATSEDKHLLIK 722

Query: 721 ELKAKGETVALLGGLTSGDVPTLMEADIGIVQENRSTKVSRLVSDLSCEDVTSLNHTLKY 780
           ELKA+G  VA L  LTS DVPTLMEADI ++QE+RS+K SR+V D+ CEDVTSLN TLK 
Sbjct: 723 ELKAEGNIVAFLPDLTSRDVPTLMEADIRLLQEHRSSKESRMVCDIMCEDVTSLNQTLKS 782

Query: 781 GRSNYLNIQKFYEVQLTALISGLLITLVCTMVSGKSPITTFHLTWVTLIMCLLGSLMMMM 840
            R  YLNIQKFY++QL A +SGL+ITLVCTMVSGKSPI T  L WVTLIMCLLG LMM+M
Sbjct: 783 SRCIYLNIQKFYQIQLIASVSGLVITLVCTMVSGKSPIATLQLIWVTLIMCLLGGLMMVM 842

Query: 841 ELNDEEVRNPVGGGNREQSLITRVILKKIVIHVLCQALVFLLLEYLGQKIMPHMEEDVRH 900
           +LNDEEV+ P+ G +R QSLIT+VIL KIVIHVLCQ  VFLL EYLGQ+IMPHMEE+VRH
Sbjct: 843 KLNDEEVQAPLQGRDRNQSLITKVILYKIVIHVLCQVFVFLLFEYLGQEIMPHMEENVRH 902

Query: 901 TMIFNTFILCQISNLLGAITVGLVTNEVVDLQVVLHILWVMISVVGVLAVQVMVIEFHGT 960
           TMIFNTF+LCQI+NLL A  +GLVTN     + VL   W ++ +V VLAVQ++VIEF   
Sbjct: 903 TMIFNTFVLCQIANLLAA--MGLVTNRGAFFKAVLQSPWFLVFLVSVLAVQMVVIEFASD 962

Query: 961 IVNAVKLSAVQWTICFLFALAL 978
           +VN VKL+AVQW+ CF FA  L
Sbjct: 963 VVNGVKLNAVQWSCCFFFAWLL 963

BLAST of HG10005493 vs. ExPASy TrEMBL
Match: A0A6J1GLZ5 (putative calcium-transporting ATPase 13, plasma membrane-type OS=Cucurbita moschata OX=3662 GN=LOC111455586 PE=4 SV=1)

HSP 1 Score: 1190.3 bits (3078), Expect = 0.0e+00
Identity = 661/985 (67.11%), Postives = 768/985 (77.97%), Query Frame = 0

Query: 1   MFQGTDDDGGAQPLLVVSAVATSYKPAGFKFRQLVLSIRFVLCLNRTRSP------QPLL 60
           MFQ T  D GA+PLLV +A          +FRQLVLSIRFVL L RT SP       P  
Sbjct: 3   MFQRTAGD-GAEPLLVGTASKL------VRFRQLVLSIRFVLSLKRTSSPPPPSPSPPPQ 62

Query: 61  HTVINVIPSESERNNKKQRLKLLVKDKDLEALHNFGGVQEAVSFLRSESEPPIDVVGDLA 120
           +TVI+V   E ER+ +KQRLK +VK+K+L+AL +FGGV+EAVSFLRSES   ID     A
Sbjct: 63  YTVIDV-HFEDERDIQKQRLKKIVKEKNLKALKDFGGVEEAVSFLRSESLLQID-----A 122

Query: 121 QTVHGLGFWGSLLLFVKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGLKHGWHDGV 180
           QT+ G GF GS LLFVK F   L+NSLNS TIL LVIAA LSFAI SLEQGLKHGWHDG 
Sbjct: 123 QTLRGYGFCGSFLLFVKEFCCDLWNSLNSWTILFLVIAAGLSFAIESLEQGLKHGWHDGF 182

Query: 181 GILLAVFLLVFVPSISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFDVKEGEIIH 240
           GILLAVFLLVF P I S+YRK+A+EK LLK KN L+VTV+R E+ QRVSV DV+EG+IIH
Sbjct: 183 GILLAVFLLVFFPPILSVYRKRADEKELLKNKNILQVTVERVELCQRVSVSDVREGDIIH 242

Query: 241 LKKGDRVPADGLLIKGKNLILDEVINSHIDPHRNPFLFSGSVVEYGEGEMIAVSIDHDTA 300
           LKKGDRVPADGLLI GKNL+LDEVIN HIDP+RNPFLF GSVVE+GEG+MIAVS+ HDTA
Sbjct: 243 LKKGDRVPADGLLISGKNLLLDEVINPHIDPNRNPFLFCGSVVEHGEGKMIAVSVGHDTA 302

Query: 301 FRKGLLDVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLLREKHKHGDYYN 360
             K L +++ HP+QE LFQSR+NKPY+F+EKFSL VSL +L+VVLVRLL +  KH DYYN
Sbjct: 303 SGKVLHELVTHPTQETLFQSRMNKPYQFLEKFSLTVSLTILVVVLVRLLCK--KHDDYYN 362

Query: 361 DKPETKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAIAVSLSFWR 420
           D+PETKGKLT   V + F RMFL+FG  R S +AT LLTMVIG+QHGMP AI +SLSFWR
Sbjct: 363 DRPETKGKLTAGIVVQAFERMFLKFGS-RASFLATVLLTMVIGLQHGMPFAITLSLSFWR 422

Query: 421 EKMRRSLKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKINPGMEFHPD 480
           EKM RSLK NC NLSACGTLGLVSAICIDITAE SFHEVE+ EFFV E+KIN  MEFH D
Sbjct: 423 EKM-RSLKGNCLNLSACGTLGLVSAICIDITAELSFHEVELCEFFVAEQKINSDMEFHTD 482

Query: 481 IHQGFEVVARVLRFDPKTFVLLRNDLLLNFWGESGLEINKESLDQRFDIIEHKFLSSEKG 540
           I Q  E+ A++L  DPK  V L NDL+     + G      + DQ+FDII+HKFLSSEKG
Sbjct: 483 ISQSLELAAQILYSDPKVSVHLGNDLVHFLKNKLG------APDQKFDIIDHKFLSSEKG 542

Query: 541 IGVLVNK-SRGDAAANLFHEHFYGDASTLLKLCSNYYDIRGRIHDIENRKDVFEKVIREM 600
           IG LV+K S  D  ANLFH H+YGDAS+++ +CS YYDIRG +HDIEN KDVFE VIREM
Sbjct: 543 IGALVSKRSEMDTEANLFHVHYYGDASSIVSMCSQYYDIRGIVHDIENEKDVFENVIREM 602

Query: 601 EEKGLRPIAFACKQ-TNDHQVFEGELKLLGLMGLKFSHEQILLALKDLENIGVRIILTSE 660
           +++GLRPIAFACKQ  ND Q+FEGE KLLG +GLKFSHE+ L AL+DL+NIG RIIL SE
Sbjct: 603 KQRGLRPIAFACKQRRNDDQLFEGESKLLGFIGLKFSHEKTLNALRDLQNIGARIILISE 662

Query: 661 DELSVAINKADGLGAQCDPNNKEVEGPRFREIMKINGREKNELMKSITVMGKATSEDKLL 720
           DEL  AIN  +GLG QCD  N  VEG RF+EIM  +G E++ELMK+I+ MGKATSEDK L
Sbjct: 663 DELFEAINMVNGLGTQCDLKNNVVEGERFKEIMMTDGTERHELMKNISFMGKATSEDKHL 722

Query: 721 LVRELKAKGETVALLGGLTSGDVPTLMEADIGIVQENRSTKVSRLVSDLSCEDVTSLNHT 780
           L++ELKA+G  VA    LTS DVPTLMEADI I+QE+RS+K SR+V D+ CEDVTSLN T
Sbjct: 723 LIKELKAEGNIVAFSPDLTSRDVPTLMEADIRILQEHRSSKESRMVGDIMCEDVTSLNQT 782

Query: 781 LKYGRSNYLNIQKFYEVQLTALISGLLITLVCTMVSGKSPITTFHLTWVTLIMCLLGSLM 840
           LK  R  YLNIQKFY++QL A +SGL+ITLVCTMVSGKSPI T  L WVTLIMCLLG LM
Sbjct: 783 LKSSRCIYLNIQKFYQIQLIASVSGLVITLVCTMVSGKSPIATLQLIWVTLIMCLLGGLM 842

Query: 841 MMMELNDEEVRNPVGGGNREQSLITRVILKKIVIHVLCQALVFLLLEYLGQKIMPHMEED 900
           M+MELNDEEV+ P+ G +R QSLIT++IL KIVIHVLCQ  VFLL EYLG++IMPHMEE+
Sbjct: 843 MVMELNDEEVQAPLQGRDRNQSLITKLILYKIVIHVLCQVFVFLLFEYLGREIMPHMEEN 902

Query: 901 VRHTMIFNTFILCQISNLLGAITVGLVTNEVVDLQVVLHILWVMISVVGVLAVQVMVIEF 960
           VRHTMIFNTF+LCQI+NLL A  +GLVTN     + VL   W +  +  VLAVQ++VIEF
Sbjct: 903 VRHTMIFNTFVLCQIANLLAA--MGLVTNSGAFFKAVLQSPWFIFFLASVLAVQMVVIEF 962

Query: 961 HGTIVNAVKLSAVQWTICFLFALAL 978
              +VN VKLSAVQW+ CF FA  L
Sbjct: 963 ASGVVNGVKLSAVQWSCCFFFAWLL 962

BLAST of HG10005493 vs. TAIR 10
Match: AT3G63380.1 (ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 366.3 bits (939), Expect = 7.7e-101
Identity = 295/966 (30.54%), Postives = 495/966 (51.24%), Query Frame = 0

Query: 58   INVIPSESERNNKKQRLKLLVKDKDLEALHNFGGVQEAVSFLRSESEPPIDVVGDLAQTV 117
            IN +P        +++L  ++K KDL  +   GGV+   + LR  + P   + G+  +  
Sbjct: 77   INSMPLSYVPAIDQEQLVEIMKGKDLPGIQALGGVEGVAASLR--TNPTKGIHGNEQEVS 136

Query: 118  HGLGFWGSLLLF---VKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGLKHGWHDGV 177
                 +GS        KG    +Y +    TIL+L++ A  S   G  E G+K GW++G 
Sbjct: 137  RRRDLFGSNTYHKPPPKGLLFFVYEAFKDLTILILLVCAIFSLGFGIKEHGIKEGWYEGG 196

Query: 178  GILLAVFLLVFVPSISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFDVKEGEIIH 237
             I +AVFL++ V ++S+ +R++ +  +L KI NN++V V R    Q +S+FDV  G+++ 
Sbjct: 197  SIFVAVFLVIVVSALSN-FRQERQFDKLSKISNNIKVEVLRDSRRQHISIFDVVVGDVVF 256

Query: 238  LKKGDRVPADGLLIKGKNLILDEVI------NSHIDPHRNPFLFSGSVVEYGEGEMIAVS 297
            LK GD++PADGL ++G +L +DE        +  +D   NPFLFSG+ +  G  +M+ VS
Sbjct: 257  LKIGDQIPADGLFLEGHSLQVDESSMTGESDHLEVDHKDNPFLFSGTKIVDGFAQMLVVS 316

Query: 298  IDHDTAFRKGLLDVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLLR-EKH 357
            +   T + + +  +    S+    Q R++     I K  L V+ ++L+V+LVR       
Sbjct: 317  VGMSTTWGQTMSSINQDSSERTPLQVRLDTLTSTIGKIGLTVAALVLVVLLVRYFTGNTE 376

Query: 358  KHGDYYNDKPETKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAIA 417
            K G    +  +T     V+ V +I               VA  +  +V+ I  G+PLA+ 
Sbjct: 377  KEGKREYNGSKTPVDTVVNSVVRI---------------VAAAVTIVVVAIPEGLPLAVT 436

Query: 418  VSLSFWREKMRRSLKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKINP 477
            ++L++  ++M  S +   R LSAC T+G  + IC D T   + +E+++ +F++G+E I+ 
Sbjct: 437  LTLAYSMKRM-MSDQAMVRKLSACETMGSATVICTDKTGTLTLNEMKVTKFWLGQESIHE 496

Query: 478  GME--FHPDI------HQGFEVVARVLRFDPKT---FVLLRNDLLLNFWGESGLEINKES 537
                   PD+        G      V   D  +   F     +  L  W    L ++ ES
Sbjct: 497  DSTKMISPDVLDLLYQGTGLNTTGSVCVSDSGSTPEFSGSPTEKALLSWTVLNLGMDMES 556

Query: 538  LDQRFDIIE-HKFLSSEKGIGVLVNKSRGDAAANLFHEHFYGDASTLLKLCSNYYDIRGR 597
            + Q+ +++    F S++K  GVLV +     + N  H H+ G A  +L +CS+YY   G 
Sbjct: 557  VKQKHEVLRVETFSSAKKRSGVLVRRK----SDNTVHVHWKGAAEMVLAMCSHYYTSTGS 616

Query: 598  IHDIEN-RKDVFEKVIREMEEKGLRPIAFACK-QTNDHQVFEGELKLLGLMGLKF-SHEQ 657
            +  +++  K   + +I+ M    LR IAFA K  +ND  + E  L L+G++GLK      
Sbjct: 617  VDLMDSTAKSRIQAIIQGMAASSLRCIAFAHKIASNDSVLEEDGLTLMGIVGLKDPCRPG 676

Query: 658  ILLALKDLENIGVRIILTSEDELSVAINKADGLGAQCDPNNKE-----VEGPRFREIMKI 717
            +  A++  +  GV I + + D +  A   A   G   D N+K+     VEG +FR     
Sbjct: 677  VSKAVETCKLAGVTIKMITGDNVFTAKAIAFECGI-LDHNDKDEEDAVVEGVQFR---NY 736

Query: 718  NGREKNELMKSITVMGKATSEDKLLLVRELKAKGETVALLGGLTSGDVPTLMEADIGIVQ 777
               E+ + +  I VM +++  DKLL+V+ L+ KG  VA+ G  T+ D P L EADIG+  
Sbjct: 737  TDEERMQKVDKIRVMARSSPSDKLLMVKCLRLKGHVVAVTGDGTN-DAPALKEADIGLSM 796

Query: 778  ENRSTKVSRLVSDLSC--EDVTSLNHTLKYGRSNYLNIQKFYEVQLTALISGLLITLVCT 837
              + T+V++  SD+    ++  S+   LK+GR  Y NIQKF + QLT  ++ L+I  +  
Sbjct: 797  GIQGTEVAKESSDIVILDDNFASVATVLKWGRCVYNNIQKFIQFQLTVNVAALVINFIAA 856

Query: 838  MVSGKSPITTFHLTWVTLIMCLLGSLMMMMELNDEEV--RNPVGGGNREQSLITRVILKK 897
            + +G+ P+T   L WV LIM  LG+L +  E    E+  R PVG   R ++LIT V+ + 
Sbjct: 857  ISAGEVPLTAVQLLWVNLIMDTLGALALATERPTNELLKRKPVG---RTEALITNVMWRN 916

Query: 898  IVIHVLCQALVFLLLEYLGQKIMPHMEEDVRHTMIFNTFILCQISNLLGAITVGLVTNEV 957
            +++  L Q  V L+L++ G  I   + ++V+ T+IFNTF+LCQ+ N   A  +     E 
Sbjct: 917  LLVQSLYQIAVLLILQFKGMSIF-SVRKEVKDTLIFNTFVLCQVFNEFNAREM-----EK 976

Query: 958  VDLQVVLHILWVMISVVGV-LAVQVMVIEFHGTIVNAVKLSAVQWTICFLFALALGWASN 989
             ++   LH   + I ++ + + +QV+++EF     + V+L+  QW  C   A +L W   
Sbjct: 977  KNVFKGLHRNRLFIGIIAITIVLQVIMVEFLKKFADTVRLNGWQWGTCIALA-SLSWPIG 1004

BLAST of HG10005493 vs. TAIR 10
Match: AT3G22910.1 (ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 359.8 bits (922), Expect = 7.2e-99
Identity = 303/970 (31.24%), Postives = 500/970 (51.55%), Query Frame = 0

Query: 50  PQPLLHTVINVIPSESERNNK--KQRLKLLVKDKDLEALHNFGGVQEAVSFLRSESEPPI 109
           P+ L +T I++     + + K   + L  LVK+K+ E L + GG    VS L+S +   I
Sbjct: 62  PRSLSYTAIDLDHHHGDDHFKIDTETLNDLVKNKNQEKLESLGGPNGLVSALKSNTRLGI 121

Query: 110 DVVGDLAQTVHGLGFWGSLLLF---VKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQ 169
           +  GD  Q       +GS        KG +  +  +    TIL+L+  A LS   G  E 
Sbjct: 122 NEEGDEIQRRRST--FGSNTYTRQPSKGLFHFVVEAFKDLTILILLGCATLSLGFGIKEH 181

Query: 170 GLKHGWHDGVGILLAVFLLVFVPSISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSV 229
           GLK GW+DG  I +AVFL+V V ++S+ +R+  +  +L K+ +N+++ V R+   Q +S+
Sbjct: 182 GLKEGWYDGGSIFVAVFLVVAVSAVSN-FRQNRQFDKLSKVSSNIKIDVVRNGRRQEISI 241

Query: 230 FDVKEGEIIHLKKGDRVPADGLLIKGKNLILDEVINSHIDPH------RNPFLFSGSVVE 289
           FD+  G+I+ L  GD+VPADG+ ++G  L +DE   +    H       N FLFSG+ + 
Sbjct: 242 FDIVVGDIVCLNIGDQVPADGVFVEGHLLHVDESSMTGESDHVEVSLTGNTFLFSGTKIA 301

Query: 290 YGEGEMIAVSIDHDTAFRKGLLDVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVV 349
            G G+M   S+  +TA+ + +  +    +++   QSR++K    I K  L+V+ ++LLV+
Sbjct: 302 DGFGKMAVTSVGMNTAWGQMMSHISRDTNEQTPLQSRLDKLTSSIGKVGLLVAFLVLLVL 361

Query: 350 LVRLL--REKHKHGDYYNDKPETKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVI 409
           L+R      K + G+   +   TK    V+ V K+               VA  +  +V+
Sbjct: 362 LIRYFTGTTKDESGNREYNGKTTKSDEIVNAVVKM---------------VAAAVTIIVV 421

Query: 410 GIQHGMPLAIAVSLSFWREKMRRSLKVNC--RNLSACGTLGLVSAICIDITAEFSFHEVE 469
            I  G+PLA+ ++L++    M+R +K N   R LSAC T+G  + IC D T   + ++++
Sbjct: 422 AIPEGLPLAVTLTLAY---SMKRMMKDNAMVRKLSACETMGSATVICTDKTGTLTLNQMK 481

Query: 470 IREFFVGEEK---INPGMEFHPDIHQGFEVVARVLRFDPKT-----FVLLRNDLLLNFWG 529
           + +F+ G E     +         HQG  +      F  K      F     +  +  W 
Sbjct: 482 VTDFWFGLESGKASSVSQRVVELFHQGVAMNTTGSVFKAKAGTEYEFSGSPTEKAILSWA 541

Query: 530 ESGLEINKESLDQRFDIIE-HKFLSSEKGIGVLVNKSRGDAAANLFHEHFYGDASTLLKL 589
              LE+  E + +  D++    F S +K  GVL+ K   +   N+   H+ G A  +L +
Sbjct: 542 VEELEMGMEKVIEEHDVVHVEGFNSEKKRSGVLMKKKGVNTENNVV--HWKGAAEKILAM 601

Query: 590 CSNYYDIRGRIHDI-ENRKDVFEKVIREMEEKGLRPIAFACKQTND--HQVFEGELKLLG 649
           CS + D  G + ++ E+ K  FEK+I+ M  K LR IAFA  + N+   ++ E +L LLG
Sbjct: 602 CSTFCDGSGVVREMKEDDKIQFEKIIQSMAAKSLRCIAFAYSEDNEDNKKLKEEKLSLLG 661

Query: 650 LMGLKF-SHEQILLALKDLENIGVRIILTSEDEL--SVAINKADGLGAQCDPNNKE--VE 709
           ++G+K      +  A++D +  GV I + + D +  + AI    G+    D  N E  +E
Sbjct: 662 IIGIKDPCRPGVKKAVEDCQFAGVNIKMITGDNIFTARAIAVECGILTPEDEMNSEAVLE 721

Query: 710 GPRFREIMKINGREKNELMKSITVMGKATSEDKLLLVRELKAKGETVALLGGLTSGDVPT 769
           G +FR   +    E+ E ++ I VM +++  DKLL+V+ LK  G  VA+ G  T+ D P 
Sbjct: 722 GEKFRNYTQ---EERLEKVERIKVMARSSPFDKLLMVKCLKELGHVVAVTGDGTN-DAPA 781

Query: 770 LMEADIGIVQENRSTKVSRLVSDLSC--EDVTSLNHTLKYGRSNYLNIQKFYEVQLTALI 829
           L EADIG+    + T+V++  SD+    ++  S+   LK+GR  Y NIQKF + QLT  +
Sbjct: 782 LKEADIGLSMGIQGTEVAKESSDIVILDDNFASVATVLKWGRCVYNNIQKFIQFQLTVNV 841

Query: 830 SGLLITLVCTMVSGKSPITTFHLTWVTLIMCLLGSLMMMME--LNDEEVRNPVGGGNREQ 889
           + L+I  V  + +G  P+T   L WV LIM  LG+L +  E   ND   + P+G   R  
Sbjct: 842 AALVINFVAAVSAGDVPLTAVQLLWVNLIMDTLGALALATEKPTNDLMKKKPIG---RVA 901

Query: 890 SLITRVILKKIVIHVLCQALVFLLLEYLGQKIMPHMEEDVRHTMIFNTFILCQISNLLGA 949
            LIT ++ + ++     Q  V L+L++ G+ I  ++ E V++T+IFNTF+LCQ+ N   A
Sbjct: 902 PLITNIMWRNLLAQAFYQISVLLVLQFRGRSIF-NVTEKVKNTLIFNTFVLCQVFNEFNA 961

Query: 950 ITVGLVTNEVVDLQVVLHILWVMISVVGVLAV-QVMVIEFHGTIVNAVKLSAVQWTICFL 980
            ++     E  ++   LH   + I ++ V  V QV+++EF     +  +L+  QW +C  
Sbjct: 962 RSL-----EKKNVFKGLHKNRLFIGIIVVTVVLQVVMVEFLKRFADTERLNLGQWGVCIA 995

BLAST of HG10005493 vs. TAIR 10
Match: AT3G21180.1 (autoinhibited Ca(2+)-ATPase 9 )

HSP 1 Score: 319.7 bits (818), Expect = 8.3e-87
Identity = 286/975 (29.33%), Postives = 469/975 (48.10%), Query Frame = 0

Query: 72   QRLKLLVKDKDLEALHNFGGVQEAVSFLRSESEPPIDVVGDLAQTVHGLGFWGSLLL--- 131
            ++L  + +++++  L  +GGV+     L+S  E  I+   D  + +     +GS      
Sbjct: 133  EKLVSMTRNQNMSNLQQYGGVKGVAEKLKSNMEQGIN--EDEKEVIDRKNAFGSNTYPKK 192

Query: 132  FVKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGLKHGWHDGVGILLAVFLLVFVPS 191
              K F++ L+ +    T+++L+IAA  S A+G   +GLK GW DG  I  AV L++ V +
Sbjct: 193  KGKNFFMFLWEAWQDLTLIILIIAAVTSLALGIKTEGLKEGWLDGGSIAFAVLLVIVVTA 252

Query: 192  ISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFDVKEGEIIHLKKGDRVPADGLLI 251
            +S  YR+  + + L   K N+++ V R     ++S++DV  G++I L+ GD+VPADG+LI
Sbjct: 253  VSD-YRQSLQFQNLNDEKRNIQLEVMRGGRTVKISIYDVVVGDVIPLRIGDQVPADGVLI 312

Query: 252  KGKNLILDEVINS------HIDPHRNPFLFSGSVVEYGEGEMIAVSIDHDTAFRKGLLDV 311
             G +L +DE   +      H D  ++PFL SG  V  G G M+   +  +T +   +  +
Sbjct: 313  SGHSLAIDESSMTGESKIVHKD-QKSPFLMSGCKVADGVGNMLVTGVGINTEWGLLMASI 372

Query: 312  IVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLL--REKHKHGDYYNDKPETK 371
                 +E   Q R+N    FI    L V+L++L+ +LVR      +  +G     K  T 
Sbjct: 373  SEDTGEETPLQVRLNGLATFIGIVGLSVALVVLVALLVRYFTGTTQDTNGATQFIKGTTS 432

Query: 372  GKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAIAVSLSFWREKMRRS 431
                VD   KIF             T+A  +  +V+ +  G+PLA+ ++L++   KM   
Sbjct: 433  ISDIVDDCVKIF-------------TIAVTI--VVVAVPEGLPLAVTLTLAYSMRKMMAD 492

Query: 432  LKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKINPGMEFHPDIHQGFE 491
             K   R LSAC T+G  + IC D T   + +++ + E + G  K+              +
Sbjct: 493  -KALVRRLSACETMGSATTICSDKTGTLTLNQMTVVETYAGGSKM--------------D 552

Query: 492  VVARVLRFDPKTFVLLRNDLLLNFWGE------------SGLEINKE------SLDQRFD 551
            V        PK   L+   +  N  G             SG    K        L  +FD
Sbjct: 553  VADNPSGLHPKLVALISEGVAQNTTGNIFHPKDGGEVEISGSPTEKAILSWAYKLGMKFD 612

Query: 552  IIE------HKF-LSSEK---GIGVLVNKSRGDAAANLFHEHFYGDASTLLKLCSNYYDI 611
             I       H F  +SEK   G+ VL    RGD+   +   H+ G A  +L  C+ Y D 
Sbjct: 613  TIRSESAIIHAFPFNSEKKRGGVAVL----RGDSEVFI---HWKGAAEIVLACCTQYMDS 672

Query: 612  RGRIHDIENRKDVFEKVIREMEEKGLRPIAFACKQTNDHQV------------FEGELKL 671
             G +  IE++K+ F   I  M +  LR +A AC+    +QV             E EL L
Sbjct: 673  NGTLQSIESQKEFFRVAIDSMAKNSLRCVAIACRTQELNQVPKEQEDLDKWALPEDELIL 732

Query: 672  LGLMGLKF-SHEQILLALKDLENIGVRIILTSEDELSVAINKADGLGAQCDPNNKE---- 731
            L ++G+K      +  A++   + GV++ + + D L  A  KA  L      ++ E    
Sbjct: 733  LAIVGIKDPCRPGVREAVRICTSAGVKVRMVTGDNLQTA--KAIALECGILSSDTEAVEP 792

Query: 732  --VEGPRFREIMKINGREKNELMKSITVMGKATSEDKLLLVRELKAKGETVALLGGLTSG 791
              +EG  FRE+ +   +E+ ++ K ITVMG+++  DKLLLV+ L+  G+ VA+ G  T+ 
Sbjct: 793  TIIEGKVFRELSE---KEREQVAKKITVMGRSSPNDKLLLVQALRKNGDVVAVTGDGTN- 852

Query: 792  DVPTLMEADIGIVQENRSTKVSRLVSDLSC--EDVTSLNHTLKYGRSNYLNIQKFYEVQL 851
            D P L EADIG+      T+V++  SD+    ++  S+   +++GRS Y NIQKF + QL
Sbjct: 853  DAPALHEADIGLSMGISGTEVAKESSDIIILDDNFASVVKVVRWGRSVYANIQKFIQFQL 912

Query: 852  TALISGLLITLVCTMVSGKSPITTFHLTWVTLIMCLLGSLMMMMELNDEEV--RNPVGGG 911
            T  ++ L+I +V  M SG  P+    L WV LIM  LG+L +  E   + +  R PVG  
Sbjct: 913  TVNVAALIINVVAAMSSGDVPLKAVQLLWVNLIMDTLGALALATEPPTDHLMHRTPVG-- 972

Query: 912  NREQSLITRVILKKIVIHVLCQALVFLLLEYLGQKIMPHMEE------DVRHTMIFNTFI 971
             R + LIT ++ + +++    Q  V L+L + G  I+    E      +V++TMIFN F+
Sbjct: 973  -RREPLITNIMWRNLLVQSFYQVAVLLVLNFAGLSILGLNHENHAHAVEVKNTMIFNAFV 1032

Query: 972  LCQISNLLGAITVGLVTNEVVDLQVVLHILW--VMISVVGV-LAVQVMVIEFHGTIVNAV 976
            +CQI N   A        +  ++ V   +    + +++VGV   +Q++++ F G   + V
Sbjct: 1033 MCQIFNEFNA-------RKPDEMNVFRGVNKNPLFVAIVGVTFILQIIIVTFLGKFAHTV 1050

BLAST of HG10005493 vs. TAIR 10
Match: AT5G57110.1 (autoinhibited Ca2+ -ATPase, isoform 8 )

HSP 1 Score: 315.1 bits (806), Expect = 2.0e-85
Identity = 276/958 (28.81%), Postives = 465/958 (48.54%), Query Frame = 0

Query: 72   QRLKLLVKDKDLEALHNFGGVQEAVSFLRSESEPPIDVVGD---LAQTVHGLGFWGSLLL 131
            ++L ++ KD +  AL  +GG Q   + L++  E  I    D     +T++G   +     
Sbjct: 119  EQLVIMSKDHNSGALEQYGGTQGLANLLKTNPEKGISGDDDDLLKRKTIYGSNTYPRKK- 178

Query: 132  FVKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGLKHGWHDGVGILLAVFLLVFVPS 191
              KGF   L+++ +  T+++L++AA  S A+G   +G+K GW+DG  I  AV L++ V +
Sbjct: 179  -GKGFLRFLWDACHDLTLIILMVAAVASLALGIKTEGIKEGWYDGGSIAFAVILVIVVTA 238

Query: 192  ISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFDVKEGEIIHLKKGDRVPADGLLI 251
            +S  Y++  + + L   K N+ + V R      +S++D+  G++I L  G++VPADG+LI
Sbjct: 239  VSD-YKQSLQFQNLNDEKRNIHLEVLRGGRRVEISIYDIVVGDVIPLNIGNQVPADGVLI 298

Query: 252  KGKNLILDE--------VINSHIDPHRNPFLFSGSVVEYGEGEMIAVSIDHDTAFRKGLL 311
             G +L LDE        ++N   D +++PFL SG  V  G G M+   +  +T +   + 
Sbjct: 299  SGHSLALDESSMTGESKIVNK--DANKDPFLMSGCKVADGNGSMLVTGVGVNTEWGLLMA 358

Query: 312  DVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLL--REKHKHGDYYNDKPE 371
             +     +E   Q R+N    FI    L V+  +L+++L R      K  +G     K +
Sbjct: 359  SISEDNGEETPLQVRLNGVATFIGSIGLAVAAAVLVILLTRYFTGHTKDNNGGPQFVKGK 418

Query: 372  TKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAIAVSLSFWREKMR 431
            TK    +D V K+              TVA  +  +V+ +  G+PLA+ ++L++   KM 
Sbjct: 419  TKVGHVIDDVVKVL-------------TVAVTI--VVVAVPEGLPLAVTLTLAYSMRKMM 478

Query: 432  RSLKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKINPGM---EFHPDI 491
               K   R LSAC T+G  + IC D T   + +++ + E + G +K +           +
Sbjct: 479  AD-KALVRRLSACETMGSATTICSDKTGTLTLNQMTVVESYAGGKKTDTEQLPATITSLV 538

Query: 492  HQGFEVVARVLRFDPK-----TFVLLRNDLLLNFWGESGLEINKESLDQRFDIIE-HKFL 551
             +G         F P+      +     +  +  WG   L +N E+   +  I+    F 
Sbjct: 539  VEGISQNTTGSIFVPEGGGDLEYSGSPTEKAILGWGVK-LGMNFETARSQSSILHAFPFN 598

Query: 552  SSEKGIGVLVNKSRGDAAANLFHEHFYGDASTLLKLCSNYYDIRGRIHDI-ENRKDVFEK 611
            S +K  GV V  + G+      H H+ G +  +L  C +Y D  G +  + +++   F+ 
Sbjct: 599  SEKKRGGVAVKTADGEV-----HVHWKGASEIVLASCRSYIDEDGNVAPMTDDKASFFKN 658

Query: 612  VIREMEEKGLRPIAFACKQTNDHQVFEGE-----------LKLLGLMGLKFSHEQILLAL 671
             I +M  + LR +A A +     +V  GE           L LL ++G+K   +     +
Sbjct: 659  GINDMAGRTLRCVALAFRTYEAEKVPTGEELSKWVLPEDDLILLAIVGIK---DPCRPGV 718

Query: 672  KD----LENIGVRIILTSEDELSVAINKADGLG-AQCDPNNKE---VEGPRFREIMKING 731
            KD     +N GV++ + + D +  A   A   G    D +  E   +EG  FRE   +  
Sbjct: 719  KDSVVLCQNAGVKVRMVTGDNVQTARAIALECGILSSDADLSEPTLIEGKSFRE---MTD 778

Query: 732  REKNELMKSITVMGKATSEDKLLLVRELKAKGETVALLGGLTSGDVPTLMEADIGIVQEN 791
             E++++   I+VMG+++  DKLLLV+ L+ +G  VA+ G  T+ D P L EADIG+    
Sbjct: 779  AERDKISDKISVMGRSSPNDKLLLVQSLRRQGHVVAVTGDGTN-DAPALHEADIGLAMGI 838

Query: 792  RSTKVSRLVSDLSC--EDVTSLNHTLKYGRSNYLNIQKFYEVQLTALISGLLITLVCTMV 851
              T+V++  SD+    ++  S+   +++GRS Y NIQKF + QLT  ++ L+I +V  + 
Sbjct: 839  AGTEVAKESSDIIILDDNFASVVKVVRWGRSVYANIQKFIQFQLTVNVAALVINVVAAIS 898

Query: 852  SGKSPITTFHLTWVTLIMCLLGSLMMMMELNDEEV--RNPVGGGNREQSLITRVILKKIV 911
            SG  P+T   L WV LIM  LG+L +  E   + +  R PVG   R++ LIT ++ + ++
Sbjct: 899  SGDVPLTAVQLLWVNLIMDTLGALALATEPPTDHLMGRPPVG---RKEPLITNIMWRNLL 958

Query: 912  IHVLCQALVFLLLEYLGQKIMPHMEE------DVRHTMIFNTFILCQISNLLGA------ 971
            I  + Q  V L L + G  I+    E       V++T+IFN F+LCQ  N   A      
Sbjct: 959  IQAIYQVSVLLTLNFRGISILGLEHEVHEHATRVKNTIIFNAFVLCQAFNEFNARKPDEK 1018

BLAST of HG10005493 vs. TAIR 10
Match: AT5G57110.2 (autoinhibited Ca2+ -ATPase, isoform 8 )

HSP 1 Score: 315.1 bits (806), Expect = 2.0e-85
Identity = 276/958 (28.81%), Postives = 465/958 (48.54%), Query Frame = 0

Query: 72   QRLKLLVKDKDLEALHNFGGVQEAVSFLRSESEPPIDVVGD---LAQTVHGLGFWGSLLL 131
            ++L ++ KD +  AL  +GG Q   + L++  E  I    D     +T++G   +     
Sbjct: 119  EQLVIMSKDHNSGALEQYGGTQGLANLLKTNPEKGISGDDDDLLKRKTIYGSNTYPRKK- 178

Query: 132  FVKGFWLCLYNSLNSCTILVLVIAADLSFAIGSLEQGLKHGWHDGVGILLAVFLLVFVPS 191
              KGF   L+++ +  T+++L++AA  S A+G   +G+K GW+DG  I  AV L++ V +
Sbjct: 179  -GKGFLRFLWDACHDLTLIILMVAAVASLALGIKTEGIKEGWYDGGSIAFAVILVIVVTA 238

Query: 192  ISSLYRKKAEEKRLLKIKNNLEVTVKRHEILQRVSVFDVKEGEIIHLKKGDRVPADGLLI 251
            +S  Y++  + + L   K N+ + V R      +S++D+  G++I L  G++VPADG+LI
Sbjct: 239  VSD-YKQSLQFQNLNDEKRNIHLEVLRGGRRVEISIYDIVVGDVIPLNIGNQVPADGVLI 298

Query: 252  KGKNLILDE--------VINSHIDPHRNPFLFSGSVVEYGEGEMIAVSIDHDTAFRKGLL 311
             G +L LDE        ++N   D +++PFL SG  V  G G M+   +  +T +   + 
Sbjct: 299  SGHSLALDESSMTGESKIVNK--DANKDPFLMSGCKVADGNGSMLVTGVGVNTEWGLLMA 358

Query: 312  DVIVHPSQEMLFQSRINKPYEFIEKFSLVVSLMMLLVVLVRLL--REKHKHGDYYNDKPE 371
             +     +E   Q R+N    FI    L V+  +L+++L R      K  +G     K +
Sbjct: 359  SISEDNGEETPLQVRLNGVATFIGSIGLAVAAAVLVILLTRYFTGHTKDNNGGPQFVKGK 418

Query: 372  TKGKLTVDFVAKIFGRMFLEFGKFRVSTVATFLLTMVIGIQHGMPLAIAVSLSFWREKMR 431
            TK    +D V K+              TVA  +  +V+ +  G+PLA+ ++L++   KM 
Sbjct: 419  TKVGHVIDDVVKVL-------------TVAVTI--VVVAVPEGLPLAVTLTLAYSMRKMM 478

Query: 432  RSLKVNCRNLSACGTLGLVSAICIDITAEFSFHEVEIREFFVGEEKINPGM---EFHPDI 491
               K   R LSAC T+G  + IC D T   + +++ + E + G +K +           +
Sbjct: 479  AD-KALVRRLSACETMGSATTICSDKTGTLTLNQMTVVESYAGGKKTDTEQLPATITSLV 538

Query: 492  HQGFEVVARVLRFDPK-----TFVLLRNDLLLNFWGESGLEINKESLDQRFDIIE-HKFL 551
             +G         F P+      +     +  +  WG   L +N E+   +  I+    F 
Sbjct: 539  VEGISQNTTGSIFVPEGGGDLEYSGSPTEKAILGWGVK-LGMNFETARSQSSILHAFPFN 598

Query: 552  SSEKGIGVLVNKSRGDAAANLFHEHFYGDASTLLKLCSNYYDIRGRIHDI-ENRKDVFEK 611
            S +K  GV V  + G+      H H+ G +  +L  C +Y D  G +  + +++   F+ 
Sbjct: 599  SEKKRGGVAVKTADGEV-----HVHWKGASEIVLASCRSYIDEDGNVAPMTDDKASFFKN 658

Query: 612  VIREMEEKGLRPIAFACKQTNDHQVFEGE-----------LKLLGLMGLKFSHEQILLAL 671
             I +M  + LR +A A +     +V  GE           L LL ++G+K   +     +
Sbjct: 659  GINDMAGRTLRCVALAFRTYEAEKVPTGEELSKWVLPEDDLILLAIVGIK---DPCRPGV 718

Query: 672  KD----LENIGVRIILTSEDELSVAINKADGLG-AQCDPNNKE---VEGPRFREIMKING 731
            KD     +N GV++ + + D +  A   A   G    D +  E   +EG  FRE   +  
Sbjct: 719  KDSVVLCQNAGVKVRMVTGDNVQTARAIALECGILSSDADLSEPTLIEGKSFRE---MTD 778

Query: 732  REKNELMKSITVMGKATSEDKLLLVRELKAKGETVALLGGLTSGDVPTLMEADIGIVQEN 791
             E++++   I+VMG+++  DKLLLV+ L+ +G  VA+ G  T+ D P L EADIG+    
Sbjct: 779  AERDKISDKISVMGRSSPNDKLLLVQSLRRQGHVVAVTGDGTN-DAPALHEADIGLAMGI 838

Query: 792  RSTKVSRLVSDLSC--EDVTSLNHTLKYGRSNYLNIQKFYEVQLTALISGLLITLVCTMV 851
              T+V++  SD+    ++  S+   +++GRS Y NIQKF + QLT  ++ L+I +V  + 
Sbjct: 839  AGTEVAKESSDIIILDDNFASVVKVVRWGRSVYANIQKFIQFQLTVNVAALVINVVAAIS 898

Query: 852  SGKSPITTFHLTWVTLIMCLLGSLMMMMELNDEEV--RNPVGGGNREQSLITRVILKKIV 911
            SG  P+T   L WV LIM  LG+L +  E   + +  R PVG   R++ LIT ++ + ++
Sbjct: 899  SGDVPLTAVQLLWVNLIMDTLGALALATEPPTDHLMGRPPVG---RKEPLITNIMWRNLL 958

Query: 912  IHVLCQALVFLLLEYLGQKIMPHMEE------DVRHTMIFNTFILCQISNLLGA------ 971
            I  + Q  V L L + G  I+    E       V++T+IFN F+LCQ  N   A      
Sbjct: 959  IQAIYQVSVLLTLNFRGISILGLEHEVHEHATRVKNTIIFNAFVLCQAFNEFNARKPDEK 1018