HG10005221 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10005221 vs. NCBI nr
Match: XP_038888983.1 (taxadiene 5-alpha hydroxylase [Benincasa hispida])

HSP 1 Score: 844.7 bits (2181), Expect = 4.2e-241
Identity = 435/515 (84.47%), Postives = 451/515 (87.57%), Query Frame = 0

Query: 14  MLSFLAAMLIISIILVLRSNKRSKNVPPGQMGLPWIGETMAFYRAQRKNRVFEDFIGPRV 73
           MLSF AAMLIISI  V+RS KRSKNVPPGQMGLPWIGETMAFYRAQ KNR+FEDF+ PRV
Sbjct: 1   MLSFFAAMLIISIAFVIRSKKRSKNVPPGQMGLPWIGETMAFYRAQSKNRLFEDFVEPRV 60

Query: 74  AKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGER 133
           AKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQLMGEECIMQKQGE 
Sbjct: 61  AKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGES 120

Query: 134 HRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECL 193
           HRFVR+LIAASLSTSSLEVMMPKL  TI+VYLDTKWVG DTIGLFHSAKVLTFT+VFECL
Sbjct: 121 HRFVRSLIAASLSTSSLEVMMPKLFTTIRVYLDTKWVGRDTIGLFHSAKVLTFTVVFECL 180

Query: 194 MGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIEKMLWEVVTEKRRRIEGK 253
           MGIKVEPRVLTTFERVLEGVFAPP RFPGTRFSRAKKARLEIEKML EVV EKRRRIEGK
Sbjct: 181 MGIKVEPRVLTTFERVLEGVFAPPIRFPGTRFSRAKKARLEIEKMLREVVREKRRRIEGK 240

Query: 254 LEEEEEECGTLLSRMVRAMIRGEISEMEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHSN 313
           L EEEEECG+LLSRMVRAMIRGEISEMEVIDNVVL VFAAHDTTSFAIAMTFKMLA H+N
Sbjct: 241 L-EEEEECGSLLSRMVRAMIRGEISEMEVIDNVVLFVFAAHDTTSFAIAMTFKMLAHHTN 300

Query: 314 CYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFPPIFGSFRKAISDIEY 373
           CY LLLQE TDIARSKG +QNLTMEDIKKMKYTWQ+ARESIRLFPPIFGSFRKAISDIEY
Sbjct: 301 CYSLLLQEQTDIARSKGSNQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEY 360

Query: 374 ERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHFGCMQVLWTTYGTHYE 433
           ERFFIP+GWK                                       VLWTTYGTHY+
Sbjct: 361 ERFFIPEGWK---------------------------------------VLWTTYGTHYK 420

Query: 434 EEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILVFIHLVVTRYDWSLV 493
           EEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNIL+FIHLVVTRYDW+LV
Sbjct: 421 EEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWNLV 475

Query: 494 HTDEPIIMDPLPVPSHGMPIKISPKSEVCPNVLQM 529
           H DEPIIMDPLPVPS GMP+KIS KS+VCPN  QM
Sbjct: 481 HPDEPIIMDPLPVPSQGMPMKISLKSDVCPNDHQM 475

BLAST of HG10005221 vs. NCBI nr
Match: XP_008449518.1 (PREDICTED: taxadiene 5-alpha hydroxylase [Cucumis melo])

HSP 1 Score: 840.5 bits (2170), Expect = 8.0e-240
Identity = 428/516 (82.95%), Postives = 450/516 (87.21%), Query Frame = 0

Query: 14  MLSFLAA-MLIISIILVLRSNKRSKNVPPGQMGLPWIGETMAFYRAQRKNRVFEDFIGPR 73
           MLSFLAA +LII+I  VLRSNKRSKNVPPG+MGLPWIGETMAFYRAQ KNR+FEDF+ PR
Sbjct: 1   MLSFLAAILLIITIAFVLRSNKRSKNVPPGEMGLPWIGETMAFYRAQSKNRLFEDFVEPR 60

Query: 74  VAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGE 133
           VAKYGKIFKT+LMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQLMGEECIMQK+GE
Sbjct: 61  VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 120

Query: 134 RHRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFEC 193
           +HR VR LIA++LSTSSLEVMMPKLCNTIQVYLDTKWV CDT+GLF SAKVLTFTIVFEC
Sbjct: 121 KHRCVRGLIASALSTSSLEVMMPKLCNTIQVYLDTKWVDCDTVGLFRSAKVLTFTIVFEC 180

Query: 194 LMGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIEKMLWEVVTEKRRRIEG 253
           L+GIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIE ML EVV EKRR+IEG
Sbjct: 181 LLGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIETMLREVVREKRRKIEG 240

Query: 254 KLEEEEEECGTLLSRMVRAMIRGEISEMEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHS 313
           KL EEEE+CG+LLSRMVRAMI GEISEMEV+DN+VLLVFAAHDTTSFAIAMTFKML+QH+
Sbjct: 241 KL-EEEEQCGSLLSRMVRAMIGGEISEMEVVDNIVLLVFAAHDTTSFAIAMTFKMLSQHT 300

Query: 314 NCYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFPPIFGSFRKAISDIE 373
           NCY LLLQEH DI RSKGP QNLTMEDIKKMKYTWQ+ARESIRLFPPIFGSFRKAISDIE
Sbjct: 301 NCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIE 360

Query: 374 YERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHFGCMQVLWTTYGTHY 433
           YE F IPKGWK                                       VLWTTYGTHY
Sbjct: 361 YESFLIPKGWK---------------------------------------VLWTTYGTHY 420

Query: 434 EEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILVFIHLVVTRYDWSL 493
            EEYF DPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNIL+FIH VVTRYDWSL
Sbjct: 421 GEEYFGDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSL 476

Query: 494 VHTDEPIIMDPLPVPSHGMPIKISPKSEVCPNVLQM 529
           VH DEPIIMDPLPVP HGMPIKISPKSE+CPNV QM
Sbjct: 481 VHPDEPIIMDPLPVPCHGMPIKISPKSEICPNVHQM 476

BLAST of HG10005221 vs. NCBI nr
Match: XP_004140249.1 (taxadiene 5-alpha hydroxylase [Cucumis sativus] >KAE8647348.1 hypothetical protein Csa_003098 [Cucumis sativus])

HSP 1 Score: 834.7 bits (2155), Expect = 4.4e-238
Identity = 427/516 (82.75%), Postives = 447/516 (86.63%), Query Frame = 0

Query: 14  MLSFLAA-MLIISIILVLRSNKRSKNVPPGQMGLPWIGETMAFYRAQRKNRVFEDFIGPR 73
           MLSFLAA +LIISI  VLRS KRS+NVPPG MGLPWIGETM FYRAQ KNR+FEDF+ PR
Sbjct: 1   MLSFLAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPR 60

Query: 74  VAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGE 133
           VAKYGKIFKT+LMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQLMGEECIMQK+GE
Sbjct: 61  VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 120

Query: 134 RHRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFEC 193
           +HR VR LIAA+LSTSSL+VMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFEC
Sbjct: 121 KHRCVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFEC 180

Query: 194 LMGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIEKMLWEVVTEKRRRIEG 253
           L+GI+VEPRVLT FERVLEGVFAPPFRFPGTRFSRAKKARLEIE  L EVV EKRR+IEG
Sbjct: 181 LLGIQVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEG 240

Query: 254 KLEEEEEECGTLLSRMVRAMIRGEISEMEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHS 313
           KL  EEEECG+LLSRMVRAMI GEISEMEVIDN+VLLVFAAHDTTSFAIAMTFKML+QH+
Sbjct: 241 KL--EEEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHT 300

Query: 314 NCYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFPPIFGSFRKAISDIE 373
           NCY LLLQEHTDI RSKGP QNLTMEDIKKMKYTWQ+ARESIRLFPPIFGSFRKAISDIE
Sbjct: 301 NCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIE 360

Query: 374 YERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHFGCMQVLWTTYGTHY 433
           YE F IPKGWK                                       VLWTTYGTHY
Sbjct: 361 YESFLIPKGWK---------------------------------------VLWTTYGTHY 420

Query: 434 EEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILVFIHLVVTRYDWSL 493
            EEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNIL+F+H VVTRYDWSL
Sbjct: 421 GEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFVHSVVTRYDWSL 475

Query: 494 VHTDEPIIMDPLPVPSHGMPIKISPKSEVCPNVLQM 529
           VH DEPIIMDPLPVP HGMPIKISPKS+VC NV QM
Sbjct: 481 VHPDEPIIMDPLPVPYHGMPIKISPKSQVCSNVHQM 475

BLAST of HG10005221 vs. NCBI nr
Match: XP_023554746.1 (taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 814.3 bits (2102), Expect = 6.1e-232
Identity = 423/528 (80.11%), Postives = 444/528 (84.09%), Query Frame = 0

Query: 1   MLNEFSSFLLTIKMLSFLAAMLIISIILVLRSNKRSKNVPPGQMGLPWIGETMAFYRAQR 60
           M+ EFS FLL   M  FL+A+LIISII V RS K SK VPPGQMGLPWIGETMAFYRAQR
Sbjct: 1   MVQEFSCFLLA-TMFLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQR 60

Query: 61  KNRVFEDFIGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQL 120
           KNR+FE+F+GPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQL
Sbjct: 61  KNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQL 120

Query: 121 MGEECIMQKQGERHRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHS 180
           MGEECIMQKQGE HR VR LIAASL++SSLE MMPKLC TIQ+YLDTKW+G D I L+HS
Sbjct: 121 MGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHS 180

Query: 181 AKVLTFTIVFECLMGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIEKMLW 240
           AKVLTFTIVFECLMGIKVEPR+LTTFERVLEGVFAPPF FPGTRFSRAKKAR+EI KML 
Sbjct: 181 AKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLR 240

Query: 241 EVVTEKRRRIEGKLEEEEEECGTLLSRMVRAMIRGEISEMEVIDNVVLLVFAAHDTTSFA 300
           EVV EKRRRIE   E EEEEC  LLSRMVRAMIRGEISE+EV+DNVVLLVFAAHDTTSFA
Sbjct: 241 EVVREKRRRIEE--EPEEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFA 300

Query: 301 IAMTFKMLAQHSNCYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFPPI 360
           IAMTFKMLAQ  NCY LLLQEH DI RSKG  QN+TMEDIKKMKYTWQ+ARESIRLFPPI
Sbjct: 301 IAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIRLFPPI 360

Query: 361 FGSFRKAISDIEYERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHFGC 420
           FGSFRKAI+DIEYE FFIPKGWK                                     
Sbjct: 361 FGSFRKAITDIEYEGFFIPKGWK------------------------------------- 420

Query: 421 MQVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILVF 480
             VLWTTYGTH E+EYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNIL+F
Sbjct: 421 --VLWTTYGTHLEDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIF 480

Query: 481 IHLVVTRYDWSLVHTDEPIIMDPLPVPSHGMPIKISPKSEVCPNVLQM 529
           IHLVVTRYDWSLVH DE IIMDPLP+PSHGMPIKISPKSE  PNV QM
Sbjct: 481 IHLVVTRYDWSLVHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM 486

BLAST of HG10005221 vs. NCBI nr
Match: KAG7012036.1 (hypothetical protein SDJN02_26944, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 813.5 bits (2100), Expect = 1.0e-231
Identity = 421/528 (79.73%), Postives = 444/528 (84.09%), Query Frame = 0

Query: 1   MLNEFSSFLLTIKMLSFLAAMLIISIILVLRSNKRSKNVPPGQMGLPWIGETMAFYRAQR 60
           M+ EFS FLL   M  FL+A+LIISI+ V RS K SK VPPGQMGLPWIGETMAFYRAQR
Sbjct: 1   MVQEFSCFLLA-TMFLFLSAILIISIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQR 60

Query: 61  KNRVFEDFIGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQL 120
           KNR+FE+F+GPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQL
Sbjct: 61  KNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQL 120

Query: 121 MGEECIMQKQGERHRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHS 180
           MGEECIMQKQGE HR  R LIAASL++SSLE MMPKLC TIQ+YLDTKWVG D I L+HS
Sbjct: 121 MGEECIMQKQGESHRCARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHS 180

Query: 181 AKVLTFTIVFECLMGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIEKMLW 240
           AKVLTFTIVFECLMGIKVEPR+LTTFERVLEGVFAPPF FPGTRFSRAKKAR+EI KML 
Sbjct: 181 AKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLR 240

Query: 241 EVVTEKRRRIEGKLEEEEEECGTLLSRMVRAMIRGEISEMEVIDNVVLLVFAAHDTTSFA 300
           EVV EKRRRIE   E E EEC  LLSRMVRAMIRGEISE+EV+DNVVLLVFAAHDTTSFA
Sbjct: 241 EVVREKRRRIEE--EPEGEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFA 300

Query: 301 IAMTFKMLAQHSNCYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFPPI 360
           IA+TFKMLAQ  NCY LLLQEH DI RSKGP QNLTMEDIKKMKYTWQ+ARESIRLFPPI
Sbjct: 301 IAVTFKMLAQQPNCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPI 360

Query: 361 FGSFRKAISDIEYERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHFGC 420
           FGSFRKAI+DIEYE FFIPKGWK                                     
Sbjct: 361 FGSFRKAITDIEYEGFFIPKGWK------------------------------------- 420

Query: 421 MQVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILVF 480
             VLWTTYGTH ++EYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNIL+F
Sbjct: 421 --VLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIF 480

Query: 481 IHLVVTRYDWSLVHTDEPIIMDPLPVPSHGMPIKISPKSEVCPNVLQM 529
           IHLVVTRYDWSL+H DEPIIMDPLP+PSHGMPIKISPKSE  PNV QM
Sbjct: 481 IHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISPKSESHPNVQQM 486

BLAST of HG10005221 vs. ExPASy Swiss-Prot
Match: Q6WG30 (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2)

HSP 1 Score: 320.1 bits (819), Expect = 4.7e-86
Identity = 184/514 (35.80%), Postives = 295/514 (57.39%), Query Frame = 0

Query: 15  LSFLAAMLIISIILVLRSNKRSK-NVPPGQMGLPWIGETMAFYRAQRKNRVFEDFIGPRV 74
           LS +A +L+  ++L+ RS + S   +PPG++G+P+IGE+  F RA R N + E F   RV
Sbjct: 29  LSAIAGILL--LLLLFRSKRHSSLKLPPGKLGIPFIGESFIFLRALRSNSL-EQFFDERV 88

Query: 75  AKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGER 134
            K+G +FKTSL+G PTVV+ G   NR  LSNE KLV  SWP+  ++LMGE  +  ++GE 
Sbjct: 89  KKFGLVFKTSLIGHPTVVLCGPAGNRLILSNEEKLVQMSWPAQFMKLMGENSVATRRGED 148

Query: 135 HRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTF---TIVF 194
           H  +R+ +A      +L+  + K+   IQ +++ KW G D + +    + L F    I+F
Sbjct: 149 HIVMRSALAGFFGPGALQSYIGKMNTEIQSHINEKWKGKDEVNVLPLVRELVFNISAILF 208

Query: 195 ECLMGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIEKMLWEVVTEKRRRI 254
             +   + + R+    E +L G FA P   PG  F RA + R ++ K++  ++ +++  +
Sbjct: 209 FNIYDKQEQDRLHKLLETILVGSFALPIDLPGFGFHRALQGRAKLNKIMLSLIKKRKEDL 268

Query: 255 E-GKLEEEEEECGTLLSRMVRAMIRGEISEMEVIDNVVLLVFAAHDTTSFAIAMTFKMLA 314
           + G     ++    LL+   R      ++  E++DN   L+ A++DTT+  +A+ FK+L+
Sbjct: 269 QSGSATATQDLLSVLLT--FRDDKGTPLTNDEILDNFSSLLHASYDTTTSPMALIFKLLS 328

Query: 315 QHSNCYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFPPIFGSFRKAIS 374
            +  CY  ++QE  +I  +K   + +T +D+K MKYTWQ+A+E++R+FPP+FG+FRKAI+
Sbjct: 329 SNPECYQKVVQEQLEILSNKEEGEEITWKDLKAMKYTWQVAQETLRMFPPVFGTFRKAIT 388

Query: 375 DIEYERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHFGCMQVLWTTYG 434
           DI+Y+ + IPKGWK                                       +LWTTY 
Sbjct: 389 DIQYDGYTIPKGWK---------------------------------------LLWTTYS 448

Query: 435 THYEEEYFEDPLSFKPSRFEEP---VSQYVYVPFGGGPRACAGYQLAKLNILVFI-HLVV 494
           TH ++ YF +P  F PSRF++    V+ Y ++PFGGG R+C G++ +K+ IL+F+ H V 
Sbjct: 449 THPKDLYFNEPEKFMPSRFDQEGKHVAPYTFLPFGGGQRSCVGWEFSKMEILLFVHHFVK 498

Query: 495 TRYDWSLVHTDEPIIMDPL-PVPSHGMPIKISPK 519
           T   ++ V  DE I  DPL P+PS G  IK+ P+
Sbjct: 509 TFSSYTPVDPDEKISGDPLPPLPSKGFSIKLFPR 498

BLAST of HG10005221 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 2.6e-84
Identity = 188/514 (36.58%), Postives = 279/514 (54.28%), Query Frame = 0

Query: 17  FLAAMLIISIILVLRSNKRSK---NVPPGQMGLPWIGETMAFYRAQRKNRVFEDFIGPRV 76
           FLA+ + ++    L SN R+K    +PPG  G P IGET+ F   QRK     DF   R 
Sbjct: 26  FLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNP-RDFFDSRT 85

Query: 77  AKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGER 136
            KYG +F TSL+G PTVV    E NRF  SNE KLVV+SWPS SV  +    ++   G+ 
Sbjct: 86  QKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPS-SVGNLFRSSLITTVGDD 145

Query: 137 HRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECL 196
            + +R ++   L   +L   + ++ +  + +L   W+G D +      K  TF++  +  
Sbjct: 146 AKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLACDLF 205

Query: 197 MGIKVEP---RVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIEKMLWEVVTEKRRRI 256
             I  +    R+   F   ++GV   P   PGTR+++AK A   I + L  ++ E++  +
Sbjct: 206 ASINNKDDLGRLWLHFMVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINERKIGL 265

Query: 257 E-GKLEEEEEECGTLLSRMVRAMIRGE-ISEMEVIDNVVLLVFAAHDTTSFAIAMTFKML 316
           E G    E++    LLS +     +GE +++ E+ DN++LL++A HDT+S  + +  K L
Sbjct: 266 EAGNASPEQDLLSFLLSNVDE---QGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFL 325

Query: 317 AQHSNCYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFPPIFGSFRKAI 376
           A++ +CY  +L+E  DIA SK   Q L  ED+++MKY+W++A+E++RLFP   GSFRKAI
Sbjct: 326 AENPHCYEEVLREQLDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGSFRKAI 385

Query: 377 SDIEYERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHFGCMQVLWTTY 436
            +  Y+ F IPKGWK                                       + WT  
Sbjct: 386 KEFIYDGFTIPKGWK---------------------------------------MYWTVN 445

Query: 437 GTHYEEEYFEDPLSFKPSRFE-EPVSQYVYVPFGGGPRACAGYQLAKLNILVFIHLVVTR 496
            TH + EYF +P +F PSRFE E    Y +VPFGGGPR C G + A+L ILVF+H +V  
Sbjct: 446 STHRKSEYFSNPETFDPSRFEGEGPPPYTFVPFGGGPRMCPGNEFARLEILVFLHNIVKN 495

Query: 497 YDWSLVHTDEPIIMDPLPVPSHGMPIKISPKSEV 522
             W+LV+  E +I+DP+P P +G+PIK+ P   V
Sbjct: 506 CKWNLVNPGEKVIVDPMPAPVNGLPIKLVPHDSV 495

BLAST of HG10005221 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 9.9e-84
Identity = 185/510 (36.27%), Postives = 280/510 (54.90%), Query Frame = 0

Query: 17  FLAAMLIISIILVLRSNKRSK---NVPPGQMGLPWIGETMAFYRAQRKNRVFEDFIGPRV 76
           FLA+ + ++    L SN R+K    +PPG  G P IGET+ F   QRK     DF   R 
Sbjct: 26  FLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNP-RDFFDSRT 85

Query: 77  AKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGER 136
            KYG +F TSL+G PTVV    E NRF  SNE KLVV+SWPS SV  +    ++   G+ 
Sbjct: 86  QKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPS-SVGNLFRSSLITTVGDD 145

Query: 137 HRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECL 196
            + +R ++   L   +L   + ++ +  + +L   W+G D +      K  TF++  +  
Sbjct: 146 AKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLACDLF 205

Query: 197 MGIKVE---PRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIEKMLWEVVTEKRRRI 256
             I  +    R+   F   ++GV   P   PGTR+++ K A   I + L  ++ E++  +
Sbjct: 206 ASINTKDDLDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINERKIAL 265

Query: 257 E-GKLEEEEEECGTLLSRMVRAMIRGE-ISEMEVIDNVVLLVFAAHDTTSFAIAMTFKML 316
           E G    E++    LLS +     +GE +++ E+ DN++LL++A HDT+S  + +  K L
Sbjct: 266 EAGNASPEQDLLSFLLSNVDE---QGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFL 325

Query: 317 AQHSNCYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFPPIFGSFRKAI 376
           A++ +CY  +L+E  +IA SK   Q L  ED+++MKY+W++A+E++RLFP + GSFRKAI
Sbjct: 326 AENPHCYEEVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGSFRKAI 385

Query: 377 SDIEYERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHFGCMQVLWTTY 436
            +  Y+ F IPKGWK           LH                            WT  
Sbjct: 386 KEFIYDGFTIPKGWK-----------LH----------------------------WTVN 445

Query: 437 GTHYEEEYFEDPLSFKPSRFE-EPVSQYVYVPFGGGPRACAGYQLAKLNILVFIHLVVTR 496
            TH + EYF +P  F PSRFE E    Y +VPFGGGPR C G + A++ IL+F+H +V  
Sbjct: 446 STHQKSEYFSNPEKFDPSRFEGEGPPPYTFVPFGGGPRMCPGNEFARMEILIFLHNIVKN 491

Query: 497 YDWSLVHTDEPIIMDPLPVPSHGMPIKISP 518
           ++W+LV+  E +I+DP+P P +G+PIK+ P
Sbjct: 506 FNWNLVNPLEKVIVDPMPAPVNGLPIKLVP 491

BLAST of HG10005221 vs. ExPASy Swiss-Prot
Match: Q8W4T9 (Taxane 13-alpha-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A2 PE=1 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 8.4e-83
Identity = 176/513 (34.31%), Postives = 281/513 (54.78%), Query Frame = 0

Query: 17  FLAAMLIISIILVLRSNKRSK-NVPPGQMGLPWIGETMAFYRAQRKNRVFEDFIGPRVAK 76
           F+   ++  IIL  RS + S   +PPG +G P +GET+ F R+   +   + FI  R++K
Sbjct: 15  FVTLAVMAGIILFFRSKRHSSVKLPPGNLGFPLVGETLQFVRSLGSSTP-QQFIEERMSK 74

Query: 77  YGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGERHR 136
           +G +FKTS++G PTVV+ G   NR  LSNE KLV  SWPS  ++L+GE+C+  K GE+HR
Sbjct: 75  FGDVFKTSIIGHPTVVLCGPAGNRLVLSNENKLVQMSWPSSMMKLIGEDCLGGKTGEQHR 134

Query: 137 FVRALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECLMG 196
            VRA +   L   +L+    K+ + IQ +++ KW G D   +    K L F++      G
Sbjct: 135 IVRAALTRFLGPQALQNHFAKMSSGIQRHINEKWKGKDEATVLPLVKDLVFSVASRLFFG 194

Query: 197 I---KVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIEKMLWEVVTEKRRRIEG 256
           I    ++ ++    E +L G F+ P   PG  + +A +AR  +  ++  ++ ++R  +  
Sbjct: 195 ITEEHLQEQLHNLLEVILVGSFSVPLNIPGFSYHKAIQARATLADIMTHLIEKRRNELRA 254

Query: 257 KLEEEEEECGTLLSRMVRAMIRG-EISEMEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQH 316
               E ++  ++L        RG  +++ E++DN  +L+  ++D+T+  + M  K+LA H
Sbjct: 255 GTASENQDLLSVLLTFTDE--RGNSLADKEILDNFSMLLHGSYDSTNSPLTMLIKVLASH 314

Query: 317 SNCYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFPPIFGSFRKAISDI 376
              Y  + QE   I  +K   + +  +D+K+MKY+WQ+ +E++R++PPIFG+FRKAI+DI
Sbjct: 315 PESYEKVAQEQFGILSTKMEGEEIAWKDLKEMKYSWQVVQETLRMYPPIFGTFRKAITDI 374

Query: 377 EYERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHFGCMQVLWTTYGTH 436
            Y  + IPKGWK                                       +LWTTY T 
Sbjct: 375 HYNGYTIPKGWK---------------------------------------LLWTTYSTQ 434

Query: 437 YEEEYFEDPLSFKPSRFEEP---VSQYVYVPFGGGPRACAGYQLAKLNILVFIHLVVTRY 496
            +EEYF+D   FKPSRFEE    V+ Y Y+PFGGG R C G++ AK+  L+F+H  V  +
Sbjct: 435 TKEEYFKDADQFKPSRFEEEGKHVTPYTYLPFGGGMRVCPGWEFAKMETLLFLHHFVKAF 485

Query: 497 D-WSLVHTDEPIIMDPL-PVPSHGMPIKISPKS 520
                +  +E +   PL P+P +G+PIK+  +S
Sbjct: 495 SGLKAIDPNEKLSGKPLPPLPVNGLPIKLYSRS 485

BLAST of HG10005221 vs. ExPASy Swiss-Prot
Match: Q84KI1 (Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 3.9e-80
Identity = 181/511 (35.42%), Postives = 281/511 (54.99%), Query Frame = 0

Query: 20  AMLIISIILVLRSNKRSK-NVPPGQMGLPWIGETMAFYRAQRKNRVFEDFIGPRVAKYGK 79
           A +++ ++L LRS +RS   +PPG++G P+IGE++ F +A R N V E F+  RV  +G 
Sbjct: 30  AGIVLPLLLFLRSKRRSSVGLPPGKLGYPFIGESLLFLKALRSNTV-EQFLDERVKNFGN 89

Query: 80  IFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGERHRFVR 139
           +FKTSL+G PTVV+ G   NR  L+NE KLV  SWP  S++LMGE+ I  K+GE H  +R
Sbjct: 90  VFKTSLIGHPTVVLCGPAGNRLILANEEKLVQMSWPKSSMKLMGEKSITAKRGEGHMIIR 149

Query: 140 ALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECLMGIKV 199
           + +    S  +L+  + ++  TI+ +++ KW G D + +      L F I       I  
Sbjct: 150 SALQGFFSPGALQKYIGQMSKTIENHINEKWKGNDQVSVVALVGDLVFDISACLFFNINE 209

Query: 200 E---PRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIEKMLWEVVTEKRRRIEGKLE 259
           +    R+    E +  GV A P   PG  + RA +AR ++  +L  ++ +++  +   L 
Sbjct: 210 KHERERLFELLEIIAVGVLAVPVDLPGFAYHRALQARSKLNAILSGLIEKRKMDLSSGLA 269

Query: 260 EEEEECGTLLSRMVRAM-IRGE-ISEMEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHSN 319
              ++   LLS  +     RG   S+ E++DN   L+  ++DTT  A+A  FK+L+ +  
Sbjct: 270 TSNQD---LLSVFLTFKDDRGNPCSDEEILDNFSGLLHGSYDTTVSAMACVFKLLSSNPE 329

Query: 320 CYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFPPIFGSFRKAISDIEY 379
           CY  ++QE   I  +K     +T +D+K MKYTWQ+ +E++RL+P IFGSFR+AI+DI Y
Sbjct: 330 CYEKVVQEQLGILSNKLEGDEITWKDVKSMKYTWQVVQETLRLYPSIFGSFRQAITDIHY 389

Query: 380 ERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHFGCMQVLWTTYGTHYE 439
             + IPKGWK                                       +LWT Y TH +
Sbjct: 390 NGYIIPKGWK---------------------------------------LLWTPYTTHPK 449

Query: 440 EEYFEDPLSFKPSRFEEP---VSQYVYVPFGGGPRACAGYQLAKLNILVFI-HLVVTRYD 499
           E YF +P  F PSRF++    V+ Y ++PFGGG R+C G++ +K+ IL+ + H V T   
Sbjct: 450 EMYFSEPEKFLPSRFDQEGKLVAPYTFLPFGGGQRSCPGWEFSKMEILLSVHHFVKTFST 497

Query: 500 WSLVHTDEPIIMDPL-PVPSHGMPIKISPKS 520
           ++ V   E I  D L P+PS+G  +K+ P+S
Sbjct: 510 FTPVDPAEIIARDSLCPLPSNGFSVKLFPRS 497

BLAST of HG10005221 vs. ExPASy TrEMBL
Match: A0A1S3BLK9 (taxadiene 5-alpha hydroxylase OS=Cucumis melo OX=3656 GN=LOC103491382 PE=3 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.9e-240
Identity = 428/516 (82.95%), Postives = 450/516 (87.21%), Query Frame = 0

Query: 14  MLSFLAA-MLIISIILVLRSNKRSKNVPPGQMGLPWIGETMAFYRAQRKNRVFEDFIGPR 73
           MLSFLAA +LII+I  VLRSNKRSKNVPPG+MGLPWIGETMAFYRAQ KNR+FEDF+ PR
Sbjct: 1   MLSFLAAILLIITIAFVLRSNKRSKNVPPGEMGLPWIGETMAFYRAQSKNRLFEDFVEPR 60

Query: 74  VAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGE 133
           VAKYGKIFKT+LMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQLMGEECIMQK+GE
Sbjct: 61  VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 120

Query: 134 RHRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFEC 193
           +HR VR LIA++LSTSSLEVMMPKLCNTIQVYLDTKWV CDT+GLF SAKVLTFTIVFEC
Sbjct: 121 KHRCVRGLIASALSTSSLEVMMPKLCNTIQVYLDTKWVDCDTVGLFRSAKVLTFTIVFEC 180

Query: 194 LMGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIEKMLWEVVTEKRRRIEG 253
           L+GIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIE ML EVV EKRR+IEG
Sbjct: 181 LLGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIETMLREVVREKRRKIEG 240

Query: 254 KLEEEEEECGTLLSRMVRAMIRGEISEMEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHS 313
           KL EEEE+CG+LLSRMVRAMI GEISEMEV+DN+VLLVFAAHDTTSFAIAMTFKML+QH+
Sbjct: 241 KL-EEEEQCGSLLSRMVRAMIGGEISEMEVVDNIVLLVFAAHDTTSFAIAMTFKMLSQHT 300

Query: 314 NCYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFPPIFGSFRKAISDIE 373
           NCY LLLQEH DI RSKGP QNLTMEDIKKMKYTWQ+ARESIRLFPPIFGSFRKAISDIE
Sbjct: 301 NCYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIE 360

Query: 374 YERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHFGCMQVLWTTYGTHY 433
           YE F IPKGWK                                       VLWTTYGTHY
Sbjct: 361 YESFLIPKGWK---------------------------------------VLWTTYGTHY 420

Query: 434 EEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILVFIHLVVTRYDWSL 493
            EEYF DPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNIL+FIH VVTRYDWSL
Sbjct: 421 GEEYFGDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHSVVTRYDWSL 476

Query: 494 VHTDEPIIMDPLPVPSHGMPIKISPKSEVCPNVLQM 529
           VH DEPIIMDPLPVP HGMPIKISPKSE+CPNV QM
Sbjct: 481 VHPDEPIIMDPLPVPCHGMPIKISPKSEICPNVHQM 476

BLAST of HG10005221 vs. ExPASy TrEMBL
Match: A0A6J1GJY9 (taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 1.6e-230
Identity = 420/528 (79.55%), Postives = 442/528 (83.71%), Query Frame = 0

Query: 1   MLNEFSSFLLTIKMLSFLAAMLIISIILVLRSNKRSKNVPPGQMGLPWIGETMAFYRAQR 60
           M+ EFS FLL   M  FL+A+LIISII V RS K SK VPPGQMGLPWIGETM FYRAQR
Sbjct: 1   MVQEFSCFLLA-TMFLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGETMVFYRAQR 60

Query: 61  KNRVFEDFIGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQL 120
           KNR+FE+F+GPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS SVQL
Sbjct: 61  KNRLFEEFVGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQL 120

Query: 121 MGEECIMQKQGERHRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHS 180
           MGEECIMQKQGE HR VR LIAASL++SSLE MMPKLC TIQ+YLDTKW+G D I L+HS
Sbjct: 121 MGEECIMQKQGESHRCVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHS 180

Query: 181 AKVLTFTIVFECLMGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIEKMLW 240
           AKVLTFTIVFECLMGIKVEPR+LTTFERVLEGVFAPPF FPGTRFSRAKKAR+EI KML 
Sbjct: 181 AKVLTFTIVFECLMGIKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLR 240

Query: 241 EVVTEKRRRIEGKLEEEEEECGTLLSRMVRAMIRGEISEMEVIDNVVLLVFAAHDTTSFA 300
           EVV EKRRRIE   E  EEEC  LLSRMVRAMIRGEISE+EV+DNVVLLVFAAHDTTSFA
Sbjct: 241 EVVREKRRRIEE--EPVEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFA 300

Query: 301 IAMTFKMLAQHSNCYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFPPI 360
           IAMTFKMLAQ  NCY LLLQEH DI RSKG  QN+TMEDIKKMKYTWQ+ARESIRLFPPI
Sbjct: 301 IAMTFKMLAQQPNCYSLLLQEHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIRLFPPI 360

Query: 361 FGSFRKAISDIEYERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHFGC 420
           FGSFRKAI+DIEYE FFIPKGWK                                     
Sbjct: 361 FGSFRKAITDIEYEGFFIPKGWK------------------------------------- 420

Query: 421 MQVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILVF 480
             VLWTTYGTH E++YFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNIL+F
Sbjct: 421 --VLWTTYGTHLEDKYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIF 480

Query: 481 IHLVVTRYDWSLVHTDEPIIMDPLPVPSHGMPIKISPKSEVCPNVLQM 529
           IHLVVTRYDWSLVH DE IIMDPLP+PSHGMPIKISPKSE  PNV QM
Sbjct: 481 IHLVVTRYDWSLVHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM 486

BLAST of HG10005221 vs. ExPASy TrEMBL
Match: A0A0A0KEB2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G423370 PE=3 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 1.7e-227
Identity = 403/485 (83.09%), Postives = 421/485 (86.80%), Query Frame = 0

Query: 44  MGLPWIGETMAFYRAQRKNRVFEDFIGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLS 103
           MGLPWIGETM FYRAQ KNR+FEDF+ PRVAKYGKIFKT+LMGSPTVVVNGAEANRFFLS
Sbjct: 1   MGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60

Query: 104 NEFKLVVSSWPSGSVQLMGEECIMQKQGERHRFVRALIAASLSTSSLEVMMPKLCNTIQV 163
           NEFKLVVSSWPS SVQLMGEECIMQK+GE+HR VR LIAA+LSTSSL+VMMPKLCNTIQV
Sbjct: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIAATLSTSSLDVMMPKLCNTIQV 120

Query: 164 YLDTKWVGCDTIGLFHSAKVLTFTIVFECLMGIKVEPRVLTTFERVLEGVFAPPFRFPGT 223
           YLDTKWVGCDTIGLFHSAKVLTFTIVFECL+GI+VEPRVLT FERVLEGVFAPPFRFPGT
Sbjct: 121 YLDTKWVGCDTIGLFHSAKVLTFTIVFECLLGIQVEPRVLTMFERVLEGVFAPPFRFPGT 180

Query: 224 RFSRAKKARLEIEKMLWEVVTEKRRRIEGKLEEEEEECGTLLSRMVRAMIRGEISEMEVI 283
           RFSRAKKARLEIE  L EVV EKRR+IEGKL  EEEECG+LLSRMVRAMI GEISEMEVI
Sbjct: 181 RFSRAKKARLEIETTLREVVREKRRKIEGKL--EEEECGSLLSRMVRAMIGGEISEMEVI 240

Query: 284 DNVVLLVFAAHDTTSFAIAMTFKMLAQHSNCYPLLLQEHTDIARSKGPSQNLTMEDIKKM 343
           DN+VLLVFAAHDTTSFAIAMTFKML+QH+NCY LLLQEHTDI RSKGP QNLTMEDIKKM
Sbjct: 241 DNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKM 300

Query: 344 KYTWQIARESIRLFPPIFGSFRKAISDIEYERFFIPKGWKVKISINSSAASLHQLIPYNQ 403
           KYTWQ+ARESIRLFPPIFGSFRKAISDIEYE F IPKGWK                    
Sbjct: 301 KYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWK-------------------- 360

Query: 404 SVVFRNDHRFTSNHFGCMQVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGG 463
                              VLWTTYGTHY EEYFEDPLSFKPSRFEEPVSQYVYVPFGGG
Sbjct: 361 -------------------VLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGG 420

Query: 464 PRACAGYQLAKLNILVFIHLVVTRYDWSLVHTDEPIIMDPLPVPSHGMPIKISPKSEVCP 523
           PRACAGYQLAKLNIL+F+H VVTRYDWSLVH DEPIIMDPLPVP HGMPIKISPKS+VC 
Sbjct: 421 PRACAGYQLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSQVCS 444

Query: 524 NVLQM 529
           NV QM
Sbjct: 481 NVHQM 444

BLAST of HG10005221 vs. ExPASy TrEMBL
Match: A0A6J1HZM9 (taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468378 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 3.7e-227
Identity = 410/515 (79.61%), Postives = 435/515 (84.47%), Query Frame = 0

Query: 14  MLSFLAAMLIISIILVLRSNKRSKNVPPGQMGLPWIGETMAFYRAQRKNRVFEDFIGPRV 73
           M  FL+A+LIISII V RS K SK VPPGQMGLPWIG+TMAFYRAQRKNR+FEDF+GPRV
Sbjct: 1   MFLFLSAILIISIIFVFRSIKGSKKVPPGQMGLPWIGQTMAFYRAQRKNRLFEDFVGPRV 60

Query: 74  AKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGER 133
           AKYGKIFKTSLMGSPTVV+NGAEANRFFLSNEFKLVVSSWP+ SVQLMGEECIMQKQGE 
Sbjct: 61  AKYGKIFKTSLMGSPTVVINGAEANRFFLSNEFKLVVSSWPAASVQLMGEECIMQKQGES 120

Query: 134 HRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECL 193
           HR VR LIAASL++SSLE MMPKLC TIQ++LDTKWVG D I L+HSAKVLTF+IVFECL
Sbjct: 121 HRCVRGLIAASLASSSLEAMMPKLCTTIQLHLDTKWVGHDIISLYHSAKVLTFSIVFECL 180

Query: 194 MGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIEKMLWEVVTEKRRRIEGK 253
           MGIKVEPR+LTTFERVLEGVFA PF FPGTRFSRAKKAR+EI KML EVV EKRRRIE +
Sbjct: 181 MGIKVEPRMLTTFERVLEGVFALPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEE 240

Query: 254 LEEEEEECGTLLSRMVRAMIRGEISEMEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHSN 313
           L  EEEEC  LLSRMVRAMIRGEISE+EV+DNVVLLVFAAHDTTSFAIAMTFKMLAQH +
Sbjct: 241 L--EEEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQHPS 300

Query: 314 CYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFPPIFGSFRKAISDIEY 373
           CY LLLQEH DI RSKG  QNLTMEDIKKMKYTWQ+ARESIRLFPPIFGSFRKAI+DIEY
Sbjct: 301 CYSLLLQEHADIVRSKGAGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEY 360

Query: 374 ERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHFGCMQVLWTTYGTHYE 433
           E FFIPKGWK                                       VLWT YGTH E
Sbjct: 361 EGFFIPKGWK---------------------------------------VLWTMYGTHLE 420

Query: 434 EEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILVFIHLVVTRYDWSLV 493
           +EYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNIL+FIHLVVTRYDWSL+
Sbjct: 421 DEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLI 474

Query: 494 HTDEPIIMDPLPVPSHGMPIKISPKSEVCPNVLQM 529
           H DE IIMDPLP+PSHGM  KISPKSEV PNV QM
Sbjct: 481 HPDETIIMDPLPIPSHGMQTKISPKSEVRPNVQQM 474

BLAST of HG10005221 vs. ExPASy TrEMBL
Match: A0A6J1GK43 (taxadiene 5-alpha hydroxylase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 1.3e-224
Identity = 402/498 (80.72%), Postives = 421/498 (84.54%), Query Frame = 0

Query: 31  RSNKRSKNVPPGQMGLPWIGETMAFYRAQRKNRVFEDFIGPRVAKYGKIFKTSLMGSPTV 90
           RS K SK VPPGQMGLPWIGETM FYRAQRKNR+FE+F+GPRVAKYGKIFKTSLMGSPTV
Sbjct: 18  RSIKGSKKVPPGQMGLPWIGETMVFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTV 77

Query: 91  VVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGERHRFVRALIAASLSTSSL 150
           VVNGAEANRFFLSNEFKLVVSSWPS SVQLMGEECIMQKQGE HR VR LIAASL++SSL
Sbjct: 78  VVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSL 137

Query: 151 EVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECLMGIKVEPRVLTTFERVL 210
           E MMPKLC TIQ+YLDTKW+G D I L+HSAKVLTFTIVFECLMGIKVEPR+LTTFERVL
Sbjct: 138 EAMMPKLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVL 197

Query: 211 EGVFAPPFRFPGTRFSRAKKARLEIEKMLWEVVTEKRRRIEGKLEEEEEECGTLLSRMVR 270
           EGVFAPPF FPGTRFSRAKKAR+EI KML EVV EKRRRIE   E  EEEC  LLSRMVR
Sbjct: 198 EGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEE--EPVEEECELLLSRMVR 257

Query: 271 AMIRGEISEMEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHSNCYPLLLQEHTDIARSKG 330
           AMIRGEISE+EV+DNVVLLVFAAHDTTSFAIAMTFKMLAQ  NCY LLLQEH DI RSKG
Sbjct: 258 AMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKG 317

Query: 331 PSQNLTMEDIKKMKYTWQIARESIRLFPPIFGSFRKAISDIEYERFFIPKGWKVKISINS 390
             QN+TMEDIKKMKYTWQ+ARESIRLFPPIFGSFRKAI+DIEYE FFIPKGWK       
Sbjct: 318 AGQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWK------- 377

Query: 391 SAASLHQLIPYNQSVVFRNDHRFTSNHFGCMQVLWTTYGTHYEEEYFEDPLSFKPSRFEE 450
                                           VLWTTYGTH E++YFEDPLSFKPSRFEE
Sbjct: 378 --------------------------------VLWTTYGTHLEDKYFEDPLSFKPSRFEE 437

Query: 451 PVSQYVYVPFGGGPRACAGYQLAKLNILVFIHLVVTRYDWSLVHTDEPIIMDPLPVPSHG 510
           PVSQYVYVPFGGGPRACAGYQLAKLNIL+FIHLVVTRYDWSLVH DE IIMDPLP+PSHG
Sbjct: 438 PVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWSLVHPDETIIMDPLPIPSHG 474

Query: 511 MPIKISPKSEVCPNVLQM 529
           MPIKISPKSE  PNV QM
Sbjct: 498 MPIKISPKSEARPNVQQM 474

BLAST of HG10005221 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 578.2 bits (1489), Expect = 6.9e-165
Identity = 288/490 (58.78%), Postives = 357/490 (72.86%), Query Frame = 0

Query: 29  VLRSNKRSKNVPPGQMGLPWIGETMAFYRAQRKNRVFEDFIGPRVAKYGKIFKTSLMGSP 88
           + +  ++ K + PG+MGLPWIGETM FY+AQ+ NRVFEDF+ PR+ K+G IFKT +MGSP
Sbjct: 35  ITKKIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRIIKHGNIFKTRIMGSP 94

Query: 89  TVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGEECIMQKQGERHRFVRALIAASLSTS 148
           T+VVNGAEANR  LSNEF LVVSSWPS SVQLMG  CIM KQGE+HR +R ++A SLS  
Sbjct: 95  TIVVNGAEANRLILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGEKHRVLRGIVANSLSYI 154

Query: 149 SLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECLMGIKVEPRVLTTFER 208
            LE ++PKLC+T++ + +T+W G + I L+ SAKVLTFT+VFECL GIKVE  +L  FER
Sbjct: 155 GLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFECLYGIKVEIGMLEVFER 214

Query: 209 VLEGVFAPPFRFPGTRFSRAKKARLEIEKMLWEVVTEKRRRIEGKLEEEEEECGTLLSRM 268
           VLEGVFA P  FP ++F+RAKKARLEIE  L   V EKRR +E   E  E+   TL SR+
Sbjct: 215 VLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVREKRREME--KEGAEKPNTTLFSRL 274

Query: 269 VRAMIRGEISEMEVIDNVVLLVFAAHDTTSFAIAMTFKMLAQHSNCYPLLLQEHTDIARS 328
           V  +I+G I+E EV+DN+VLLVFAAHDTTS+A++MTFKMLAQH  C   LLQEH  I  +
Sbjct: 275 VEELIKGVITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLAQHPTCRDTLLQEHAQIKAN 334

Query: 329 KGPSQNLTMEDIKKMKYTWQIARESIRLFPPIFGSFRKAISDIEYERFFIPKGWKVKISI 388
           KG  + LT+ED+KKMKY+WQ+ RE++RL PPIFGSFRKA++DI+Y  + IPKGWK     
Sbjct: 335 KGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVADIDYGGYTIPKGWK----- 394

Query: 389 NSSAASLHQLIPYNQSVVFRNDHRFTSNHFGCMQVLWTTYGTHYEEEYFEDPLSFKPSRF 448
                                             +LWTTYGTHY  E F+DP+SF P+RF
Sbjct: 395 ----------------------------------ILWTTYGTHYNPEIFQDPMSFDPTRF 454

Query: 449 EEPVSQYVYVPFGGGPRACAGYQLAKLNILVFIHLVVTRYDWSLVHTDEPIIMDPLPVPS 508
           ++P+  Y Y+PFGGGPR CAG+QLAK++ILVF+H VVT +DWSLV+ DE I MDPLP PS
Sbjct: 455 DKPIQAYTYLPFGGGPRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPS 483

Query: 509 HGMPIKISPK 519
            GMPIKISPK
Sbjct: 515 LGMPIKISPK 483

BLAST of HG10005221 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 284.6 bits (727), Expect = 1.6e-76
Identity = 179/523 (34.23%), Postives = 274/523 (52.39%), Query Frame = 0

Query: 10  LTIKMLSFLAAMLIISIILVLR---SNKRSKNVPPGQMGLPWIGETMAFYRAQRKNRVFE 69
           + I ++ FL+++L +S++L+LR   S+    N+PPG  GLP IGE+ +F  A R+    E
Sbjct: 3   MAIMIILFLSSIL-LSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHP-E 62

Query: 70  DFIGPRVAKYGK----IFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMG 129
            FI  RV ++      +FKT L GSPT VV GA  N+F  +NE KLVVS WP  SV  + 
Sbjct: 63  KFITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPD-SVNKIF 122

Query: 130 EECIMQKQGERHRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAK 189
              +     E  R +R L++  +   +L   +  +    Q + +T+W   D + +F   K
Sbjct: 123 PSSMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTK 182

Query: 190 VLTFTIVFECLMGIKVEPRVL---TTFERVLEGVFAPPFRFPGTRFSRAKKARLEIEKML 249
             TF+I     + ++   RV      F  V  G+F+ P   PGTRF+RA KA   + K +
Sbjct: 183 KFTFSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEV 242

Query: 250 WEVVTEKRRRIE-GKLEEEEEECGTLLSRMVRAMIRGEISEMEVIDNVVLLVFAAHDTTS 309
             +V +++  ++ GK  EE +    +LS M+  M  GE  + ++ D ++ L+   HDT S
Sbjct: 243 SAIVRQRKEELKAGKALEEHD----ILSHML--MNIGETKDEDLADKIIGLLIGGHDTAS 302

Query: 310 FAIAMTFKMLAQHSNCYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFP 369
                    LA+  + Y  +LQE  +I + K   + L  EDI+KM+Y+W +A E +R+ P
Sbjct: 303 IVCTFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVP 362

Query: 370 PIFGSFRKAISDIEYERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHF 429
           P+ G+FR+AI    ++ F+IPKGWK                                   
Sbjct: 363 PLSGTFREAIDHFSFKGFYIPKGWK----------------------------------- 422

Query: 430 GCMQVLWTTYGTHYEEEYFEDPLSFKPSRFE-EPVSQYVYVPFGGGPRACAGYQLAKLNI 489
               + W+   TH   +YF +P  F+P+RFE      Y YVPFGGGPR C G + A+L I
Sbjct: 423 ----LYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVPFGGGPRMCPGKEYARLEI 477

Query: 490 LVFIHLVVTRYDWSLVHTDE-PIIMDPLPVPSHGMPIKISPKS 520
           L+F+H +V R+ W  V  +E  I++DPLP+P  G+PI+I P+S
Sbjct: 483 LIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFPQS 477

BLAST of HG10005221 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 233.8 bits (595), Expect = 3.2e-61
Identity = 156/525 (29.71%), Postives = 256/525 (48.76%), Query Frame = 0

Query: 3   NEFSSFLLTIKMLSFLAAMLIISIILV---LRSNKRSKNVPPGQMGLPWIGETMAFYRAQ 62
           N FSS       L  L  ++++ ++L    L   ++   +PPG MGLP+IGET+  Y   
Sbjct: 10  NFFSSLYADEPALITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTEN 69

Query: 63  RKNRVFEDFIGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQ 122
             +     F   R  KYG IFKT ++G P V+++  EA R  L ++  L   ++P    +
Sbjct: 70  PNS-----FFATRQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKER 129

Query: 123 LMGEECIMQKQGERHRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDT--KWVGCDTIGL 182
           ++G E +   QG  H  ++ L+ +S   S+L    P + +   + L T   W    +I  
Sbjct: 130 MIGPEALFFHQGPYHSTLKRLVQSSFMPSALR---PTVSHIELLVLQTLSSWTSQKSINT 189

Query: 183 FHSAKVLTFTIVFECLMGIKVEPRVLTT----FERVLEGVFAPPFRFPGTRFSRAKKARL 242
               K   F +      G K EP  +      ++R+  G  + P   PGT F ++ KAR+
Sbjct: 190 LEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARI 249

Query: 243 EIEKMLWEVVTEKRRRIEGKLEEEEEECGTLLSRMVRAMIRGEISEMEVIDNVVLLVFAA 302
           E+ + L +V+  ++RR  G+  EE    G LL    +   R  +S+ ++ DN++ ++FAA
Sbjct: 250 ELSEELRKVI--EKRRENGR--EEGGLLGVLLG--AKDQKRNGLSDSQIADNIIGVIFAA 309

Query: 303 HDTTSFAIAMTFKMLAQHSNCYPLLLQEHTDIARS-KGPSQNLTMEDIKKMKYTWQIARE 362
            DTT+  +    K L  H N    + +E   I +  K  ++ ++ ED +KM  T ++ +E
Sbjct: 310 TDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQE 369

Query: 363 SIRLFPPIFGSFRKAISDIEYERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHR 422
           ++R    +  +FR+A+ D+EY+ + IPKGWKV              +P     +FR    
Sbjct: 370 TLRAASVLSFTFREAVQDVEYDGYLIPKGWKV--------------LP-----LFRR--- 429

Query: 423 FTSNHFGCMQVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQL 482
                             H+  E+F DP  F PSRFE     Y Y+PFG G  +C G +L
Sbjct: 430 -----------------IHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSEL 481

Query: 483 AKLNILVFIHLVVTRYDWSLVHTDEPIIMDPLPVPSHGMPIKISP 518
           AKL +L+ +H + T + W ++  +E I   P PVP  G+PI+++P
Sbjct: 490 AKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

BLAST of HG10005221 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 233.8 bits (595), Expect = 3.2e-61
Identity = 156/525 (29.71%), Postives = 256/525 (48.76%), Query Frame = 0

Query: 3   NEFSSFLLTIKMLSFLAAMLIISIILV---LRSNKRSKNVPPGQMGLPWIGETMAFYRAQ 62
           N FSS       L  L  ++++ ++L    L   ++   +PPG MGLP+IGET+  Y   
Sbjct: 10  NFFSSLYADEPALITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTEN 69

Query: 63  RKNRVFEDFIGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQ 122
             +     F   R  KYG IFKT ++G P V+++  EA R  L ++  L   ++P    +
Sbjct: 70  PNS-----FFATRQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKER 129

Query: 123 LMGEECIMQKQGERHRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDT--KWVGCDTIGL 182
           ++G E +   QG  H  ++ L+ +S   S+L    P + +   + L T   W    +I  
Sbjct: 130 MIGPEALFFHQGPYHSTLKRLVQSSFMPSALR---PTVSHIELLVLQTLSSWTSQKSINT 189

Query: 183 FHSAKVLTFTIVFECLMGIKVEPRVLTT----FERVLEGVFAPPFRFPGTRFSRAKKARL 242
               K   F +      G K EP  +      ++R+  G  + P   PGT F ++ KAR+
Sbjct: 190 LEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARI 249

Query: 243 EIEKMLWEVVTEKRRRIEGKLEEEEEECGTLLSRMVRAMIRGEISEMEVIDNVVLLVFAA 302
           E+ + L +V+  ++RR  G+  EE    G LL    +   R  +S+ ++ DN++ ++FAA
Sbjct: 250 ELSEELRKVI--EKRRENGR--EEGGLLGVLLG--AKDQKRNGLSDSQIADNIIGVIFAA 309

Query: 303 HDTTSFAIAMTFKMLAQHSNCYPLLLQEHTDIARS-KGPSQNLTMEDIKKMKYTWQIARE 362
            DTT+  +    K L  H N    + +E   I +  K  ++ ++ ED +KM  T ++ +E
Sbjct: 310 TDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQE 369

Query: 363 SIRLFPPIFGSFRKAISDIEYERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHR 422
           ++R    +  +FR+A+ D+EY+ + IPKGWKV              +P     +FR    
Sbjct: 370 TLRAASVLSFTFREAVQDVEYDGYLIPKGWKV--------------LP-----LFRR--- 429

Query: 423 FTSNHFGCMQVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQL 482
                             H+  E+F DP  F PSRFE     Y Y+PFG G  +C G +L
Sbjct: 430 -----------------IHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSEL 481

Query: 483 AKLNILVFIHLVVTRYDWSLVHTDEPIIMDPLPVPSHGMPIKISP 518
           AKL +L+ +H + T + W ++  +E I   P PVP  G+PI+++P
Sbjct: 490 AKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

BLAST of HG10005221 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 222.6 bits (566), Expect = 7.3e-58
Identity = 151/519 (29.09%), Postives = 255/519 (49.13%), Query Frame = 0

Query: 4   EFSSFLLTIKMLSFLAAMLIISIILVLRSNKRSKNVPPGQMGLPWIGETMAFYRAQRKNR 63
           +FS   LT+   +    +L   I  V RS+     +PPG MG P++GET   Y +Q  N 
Sbjct: 2   DFSGLFLTLSAAALFLCLLRF-IAGVRRSSSTKLPLPPGTMGYPYVGETFQLY-SQDPN- 61

Query: 64  VFEDFIGPRVAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSGSVQLMGE 123
               F   +  +YG +FKT ++G P V+++  EA +F L  +  L   ++P+   +++G+
Sbjct: 62  ---VFFAAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGK 121

Query: 124 ECIMQKQGERHRFVRALIAASLSTSSLEVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKV 183
           + I   QG+ H  +R L+  +    ++  M+P + +  Q  L++ W G   +  +   K 
Sbjct: 122 QAIFFHQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNS-WDGTQ-LNTYQEMKT 181

Query: 184 LTFTIVFECLMGIKVEPRVLTTFER---VLE-GVFAPPFRFPGTRFSRAKKARLEIEKML 243
            TF +    ++G K E       +R   +LE G  + P   PGT F +A KAR E+ ++L
Sbjct: 182 YTFNVALISILG-KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQIL 241

Query: 244 WEVVTEKRRRIEGKLEEEEEECGTLLSRMVRAMIRGEISEMEVIDNVVLLVFAAHDTTSF 303
             +++++R+      +        LL   +    +  +++ ++ DN++ ++FAA DTT+ 
Sbjct: 242 ANILSKRRQNPSSHTD--------LLGSFMED--KAGLTDEQIADNIIGVIFAARDTTAS 301

Query: 304 AIAMTFKMLAQHSNCYPLLLQEHTDIARSKGPSQNLTMEDIKKMKYTWQIARESIRLFPP 363
            +    K LA +      + +E   I + K   ++LT ED KKM  T+++ +E++R    
Sbjct: 302 VLTWILKYLADNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATI 361

Query: 364 IFGSFRKAISDIEYERFFIPKGWKVKISINSSAASLHQLIPYNQSVVFRNDHRFTSNHFG 423
           +  +FR+A+ D+EYE + IPKGWKV              +P     +FRN          
Sbjct: 362 LSFTFREAVEDVEYEGYLIPKGWKV--------------LP-----LFRN---------- 421

Query: 424 CMQVLWTTYGTHYEEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILV 483
                      H+  + F DP  F PSRFE       ++PFG G  +C G +LAKL I V
Sbjct: 422 ----------IHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAKLEISV 462

Query: 484 FIHLVVTRYDWSLVHTDEPIIMDPLPVPSHGMPIKISPK 519
            IH + T+Y WS+V   + I   P  +P +G+PI +  K
Sbjct: 482 LIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIALERK 462