HG10004937 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10004937
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Calcium-binding protein-like
Location	Chr08: 21545817 .. 21546131 (-)
RNA-Seq Expression	HG10004937
Synteny	HG10004937

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTTGTCAAGATCACAACTGAAGTTGTCAAGGGATGAGATTAAGGAGATCTTACAAGACCATGATATTGATGGAGATGGCACCCTCTGCAAGAAAGAGGTGGTGCAAGCCCTGCAATCTTTAGGCACTTGGACTGCGCATCAGAAAGCTCACTATGGTGTTGCGTTTGCTGACGAAGATGGTGATGGTGTCATCGATGAACATGAGTTGGACAAACTCGTCGACTATGTCATGAGGTTTCAGGCCCCAAAGGCCCCCAAGGGCCCCAAGCCCCCAAAAAAGCATTGTGACGATGGCAAATCTATTGTCTAA

mRNA sequence

ATGGCGTTGTCAAGATCACAACTGAAGTTGTCAAGGGATGAGATTAAGGAGATCTTACAAGACCATGATATTGATGGAGATGGCACCCTCTGCAAGAAAGAGGTGGTGCAAGCCCTGCAATCTTTAGGCACTTGGACTGCGCATCAGAAAGCTCACTATGGTGTTGCGTTTGCTGACGAAGATGGTGATGGTGTCATCGATGAACATGAGTTGGACAAACTCGTCGACTATGTCATGAGGTTTCAGGCCCCAAAGGCCCCCAAGGGCCCCAAGCCCCCAAAAAAGCATTGTGACGATGGCAAATCTATTGTCTAA

Coding sequence (CDS)

ATGGCGTTGTCAAGATCACAACTGAAGTTGTCAAGGGATGAGATTAAGGAGATCTTACAAGACCATGATATTGATGGAGATGGCACCCTCTGCAAGAAAGAGGTGGTGCAAGCCCTGCAATCTTTAGGCACTTGGACTGCGCATCAGAAAGCTCACTATGGTGTTGCGTTTGCTGACGAAGATGGTGATGGTGTCATCGATGAACATGAGTTGGACAAACTCGTCGACTATGTCATGAGGTTTCAGGCCCCAAAGGCCCCCAAGGGCCCCAAGCCCCCAAAAAAGCATTGTGACGATGGCAAATCTATTGTCTAA

Protein sequence

MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDGDGVIDEHELDKLVDYVMRFQAPKAPKGPKPPKKHCDDGKSIV

Homology

[Close]

BLAST of HG10004937 vs. NCBI nr
Match: KGN62514.1 (hypothetical protein Csa_021819 [Cucumis sativus])

HSP 1 Score: 143.7 bits (361), Expect = 9.1e-31
Identity = 70/110 (63.64%), Postives = 90/110 (81.82%), Query Frame = 0


Query: 1   MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADE 60
           MA+SRSQLKL+RDE++EIL++HD+DGDG+L K+EV+QAL S+G+  + QKAHYGV+ AD+
Sbjct: 1   MAMSRSQLKLTRDEVREILEEHDVDGDGSLTKQEVMQALNSMGSMMSFQKAHYGVSHADK 60

Query: 61  DGDGVID--EHELDKLVDYVMRFQAPKAPKGPKPPK----KHCDDGKSIV 105
           DGDG +D  E E++ L+DYVMRFQ P+APK PK PK    K C DGKSIV
Sbjct: 61  DGDGKVDLGEAEMENLIDYVMRFQTPRAPKTPKVPKVPAQKPCHDGKSIV 110

[Close]

BLAST of HG10004937 vs. NCBI nr
Match: KAA0060895.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 143.7 bits (361), Expect = 9.1e-31
Identity = 71/108 (65.74%), Postives = 88/108 (81.48%), Query Frame = 0


Query: 1   MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADE 60
           MA+SRSQLKL+RDE++EIL++HD+DGDG L K+EV+QAL S+G   + QKAHYGV+ AD+
Sbjct: 1   MAMSRSQLKLTRDEVREILEEHDVDGDGNLTKQEVMQALNSMGALMSFQKAHYGVSHADK 60

Query: 61  DGDGVID---EHELDKLVDYVMRFQAPKAPKGPK-PPKKHCDDGKSIV 105
           DGDG +D   E EL+KLVDYVMRFQ P+ PK PK P +K C DGKSIV
Sbjct: 61  DGDGKVDLTREDELEKLVDYVMRFQTPRTPKVPKVPAQKPCHDGKSIV 108

[Close]

BLAST of HG10004937 vs. NCBI nr
Match: KGN62521.1 (hypothetical protein Csa_021806 [Cucumis sativus])

HSP 1 Score: 123.2 bits (308), Expect = 1.3e-24
Identity = 62/95 (65.26%), Postives = 75/95 (78.95%), Query Frame = 0


Query: 1  MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADE 60
          MA SRSQLKL+RDE++EIL++HD+DGDG L K+EV+QAL S+G   + QKAHYGVAFAD+
Sbjct: 1  MATSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGVMMSFQKAHYGVAFADK 60

Query: 61 DGDGVIDEH--ELDKLVDYVMRFQAPK-APKGPKP 93
          DGDG +D    E+D LVDYVMRFQA K  PK   P
Sbjct: 61 DGDGAVDLEGGEIDSLVDYVMRFQASKDVPKDASP 95

[Close]

BLAST of HG10004937 vs. NCBI nr
Match: XP_004152805.1 (probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 96.7 bits (239), Expect = 1.3e-16
Identity = 45/81 (55.56%), Postives = 60/81 (74.07%), Query Frame = 0


Query: 2   ALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADED 61
           A+  ++L+LSR++IKEI ++HDIDGDG L   E+V+A    G+     KAHYG+A ADE+
Sbjct: 83  AIKSTRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADEN 142

Query: 62  GDGVIDEHELDKLVDYVMRFQ 83
           GDG I+E ELDKLVDY  +FQ
Sbjct: 143 GDGFIEEEELDKLVDYAQKFQ 163

[Close]

BLAST of HG10004937 vs. NCBI nr
Match: KGN62516.1 (hypothetical protein Csa_022521 [Cucumis sativus])

HSP 1 Score: 95.9 bits (237), Expect = 2.2e-16
Identity = 45/82 (54.88%), Postives = 61/82 (74.39%), Query Frame = 0


Query: 1  MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADE 60
          M+ + ++L+LSR++IKEI ++HDIDGDG L   E+V+A    G+     KAHYG+A ADE
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGVIDEHELDKLVDYVMRFQ 83
          +GDG I+E ELDKLVDY  +FQ
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQ 82

[Close]

BLAST of HG10004937 vs. ExPASy Swiss-Prot
Match: Q338P8 (Probable calcium-binding protein CML8 OS=Oryza sativa subsp. japonica OX=39947 GN=CML8 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 4.4e-04
Identity = 30/82 (36.59%), Postives = 42/82 (51.22%), Query Frame = 0


Query: 12  RDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDGDGVIDEHEL 71
           R EIKE     D DG GT+  KE+  A+++LG     ++ H  +A  D+DG G ID  E 
Sbjct: 45  RKEIKEAFDLFDTDGSGTIDPKELNVAMRALGFEMTPEQIHQMIAEVDKDGSGTIDFDEF 104

Query: 72  -----DKLVDYVMRFQAPKAPK 89
                DK+ +   R +  KA K
Sbjct: 105 VHMMTDKMGERDAREELNKAFK 126

[Close]

BLAST of HG10004937 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 7.6e-04
Identity = 22/66 (33.33%), Postives = 37/66 (56.06%), Query Frame = 0


Query: 14 EIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDGDGVIDEHELDK 73
          E+K + Q  D +GDGT+  KE+ + L+SLG +   ++    +   D +GDG +D  E  +
Sbjct: 5  ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 74 LVDYVM 80
          L   +M
Sbjct: 65 LYKTIM 70

[Close]

BLAST of HG10004937 vs. ExPASy TrEMBL
Match: A0A5A7V4S6 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00280 PE=4 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 4.4e-31
Identity = 71/108 (65.74%), Postives = 88/108 (81.48%), Query Frame = 0


Query: 1   MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADE 60
           MA+SRSQLKL+RDE++EIL++HD+DGDG L K+EV+QAL S+G   + QKAHYGV+ AD+
Sbjct: 1   MAMSRSQLKLTRDEVREILEEHDVDGDGNLTKQEVMQALNSMGALMSFQKAHYGVSHADK 60

Query: 61  DGDGVID---EHELDKLVDYVMRFQAPKAPKGPK-PPKKHCDDGKSIV 105
           DGDG +D   E EL+KLVDYVMRFQ P+ PK PK P +K C DGKSIV
Sbjct: 61  DGDGKVDLTREDELEKLVDYVMRFQTPRTPKVPKVPAQKPCHDGKSIV 108

[Close]

BLAST of HG10004937 vs. ExPASy TrEMBL
Match: A0A0A0LNG3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357860 PE=4 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 4.4e-31
Identity = 70/110 (63.64%), Postives = 90/110 (81.82%), Query Frame = 0


Query: 1   MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADE 60
           MA+SRSQLKL+RDE++EIL++HD+DGDG+L K+EV+QAL S+G+  + QKAHYGV+ AD+
Sbjct: 1   MAMSRSQLKLTRDEVREILEEHDVDGDGSLTKQEVMQALNSMGSMMSFQKAHYGVSHADK 60

Query: 61  DGDGVID--EHELDKLVDYVMRFQAPKAPKGPKPPK----KHCDDGKSIV 105
           DGDG +D  E E++ L+DYVMRFQ P+APK PK PK    K C DGKSIV
Sbjct: 61  DGDGKVDLGEAEMENLIDYVMRFQTPRAPKTPKVPKVPAQKPCHDGKSIV 110

[Close]

BLAST of HG10004937 vs. ExPASy TrEMBL
Match: A0A0A0LRC3 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G359900 PE=4 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 6.2e-25
Identity = 62/95 (65.26%), Postives = 75/95 (78.95%), Query Frame = 0


Query: 1  MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADE 60
          MA SRSQLKL+RDE++EIL++HD+DGDG L K+EV+QAL S+G   + QKAHYGVAFAD+
Sbjct: 1  MATSRSQLKLTRDEVREILEEHDVDGDGRLTKQEVMQALNSMGVMMSFQKAHYGVAFADK 60

Query: 61 DGDGVIDEH--ELDKLVDYVMRFQAPK-APKGPKP 93
          DGDG +D    E+D LVDYVMRFQA K  PK   P
Sbjct: 61 DGDGAVDLEGGEIDSLVDYVMRFQASKDVPKDASP 95

[Close]

BLAST of HG10004937 vs. ExPASy TrEMBL
Match: A0A0A0LP50 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359910 PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 4.8e-17
Identity = 46/86 (53.49%), Postives = 66/86 (76.74%), Query Frame = 0


Query: 1  MALSRSQLKLSRDE-IKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFAD 60
          M++SRSQL LSRDE IK+I +++D+DGDGTL K+EV+QA+ ++G     Q   +G++FAD
Sbjct: 1  MSVSRSQLNLSRDEMIKKIFEEYDVDGDGTLTKQEVMQAMDAMGFKILFQMTQFGISFAD 60

Query: 61 EDGDGVIDEHELDKLVDYVMRFQAPK 86
           D DG +D+ EL+KL+D V+RFQ  K
Sbjct: 61 RDDDGKVDQDELEKLIDCVIRFQTRK 86

[Close]

BLAST of HG10004937 vs. ExPASy TrEMBL
Match: A0A0A0LRC1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.1e-16
Identity = 45/82 (54.88%), Postives = 61/82 (74.39%), Query Frame = 0


Query: 1  MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADE 60
          M+ + ++L+LSR++IKEI ++HDIDGDG L   E+V+A    G+     KAHYG+A ADE
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DGDGVIDEHELDKLVDYVMRFQ 83
          +GDG I+E ELDKLVDY  +FQ
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQ 82

[Close]

BLAST of HG10004937 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 44.7 bits (104), Expect = 5.4e-05
Identity = 22/66 (33.33%), Postives = 37/66 (56.06%), Query Frame = 0


Query: 14 EIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDGDGVIDEHELDK 73
          E+K + Q  D +GDGT+  KE+ + L+SLG +   ++    +   D +GDG +D  E  +
Sbjct: 5  ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 74 LVDYVM 80
          L   +M
Sbjct: 65 LYKTIM 70

[Close]

BLAST of HG10004937 vs. TAIR 10
Match: AT3G50360.1 (centrin2 )

HSP 1 Score: 42.4 bits (98), Expect = 2.7e-04
Identity = 22/59 (37.29%), Postives = 34/59 (57.63%), Query Frame = 0


Query: 12 RDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDGDGVIDEHE 71
          + EIKE  +  D DG GT+  KE+  A+++LG     ++ +  +A  D+DG G ID  E
Sbjct: 25 KQEIKEAFELFDTDGSGTIDAKELNVAMRALGFEMTEEQINKMIADVDKDGSGAIDFDE 83

[Close]

BLAST of HG10004937 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 40.8 bits (94), Expect = 7.8e-04
Identity = 19/58 (32.76%), Postives = 34/58 (58.62%), Query Frame = 0


Query: 13  DEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDGDGVIDEHE 71
           + +K+    +DIDG+G++  +E+ + L+SLG   +  +    +   D+DGDG ID  E
Sbjct: 109 ENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEE 166

The following BLAST results are available for this feature:

BLAST of HG10004937 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN62514.1	9.1e-31	63.64	hypothetical protein Csa_021819 [Cucumis sativus]	[more]
KAA0060895.1	9.1e-31	65.74	putative calcium-binding protein CML10 [Cucumis melo var. makuwa]	[more]
KGN62521.1	1.3e-24	65.26	hypothetical protein Csa_021806 [Cucumis sativus]	[more]
XP_004152805.1	1.3e-16	55.56	probable calcium-binding protein CML10 [Cucumis sativus]	[more]
KGN62516.1	2.2e-16	54.88	hypothetical protein Csa_022521 [Cucumis sativus]	[more]

BLAST of HG10004937 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q338P8	4.4e-04	36.59	Probable calcium-binding protein CML8 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q9LNE7	7.6e-04	33.33	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]

BLAST of HG10004937 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7V4S6	4.4e-31	65.74	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0LNG3	4.4e-31	63.64	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357860 PE=4 SV=1	[more]
A0A0A0LRC3	6.2e-25	65.26	EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G359900 PE=...	[more]
A0A0A0LP50	4.8e-17	53.49	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359910 PE=4 SV=1	[more]
A0A0A0LRC1	1.1e-16	54.88	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1	[more]

BLAST of HG10004937 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05990.1	5.4e-05	33.33	EF hand calcium-binding protein family	[more]
AT3G50360.1	2.7e-04	37.29	centrin2	[more]
AT1G24620.1	7.8e-04	32.76	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 14..42 e-value: 3.5E-4 score: 29.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 10..45 score: 13.09074
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 14..75 e-value: 1.24352E-7 score: 42.9201
None	No IPR available	GENE3D	1.10.238.10		coord: 2..83 e-value: 2.0E-11 score: 45.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 84..104
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 23..35
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 59..71
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 5..77

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10004937.1	HG10004937.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding