HG10004889 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10004889
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	RING-type domain-containing protein
Location	Chr08: 21214277 .. 21217679 (+)
RNA-Seq Expression	HG10004889
Synteny	HG10004889

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAGCGGGTTTGGGGAATCTTCAAGTAGGCAACCTCAAAGCCCTTCTTGTTCCGACAACAATGGTGATGCTAGTAATTTTGAGTGCAATATTTGCCTGGATTTAGCTCAAGATCCTATTGTAACCCTTTGCGGTCATCTTTTCTGTTGGCCATGCCTTTATAAGTGGCTGCACATTCATTCCCATCCCCAGGAATGCCCAGTTTGCAAGGCTATCATAGAAGAGCAAAAATTGGTTCCTTTATATGGTAGGGGAAAGACATCGACTGACCCGAGATCAAAAAGTATTCCTGGAATCAATATTCCTAATCGTCCTGCGGGACAGAGACCTGAAGCAGCAGGTACTACCCCTGAACCAAATCCTTTTCCTCACAATGGGTTTGGATTCATGGGAGGTCTTGGAGGGTTTGCCCCAATGGCATCGACGAGGTTTGGCAATTTCACGTTGTCTGCAGCTTTTGGTGGTCTTGTCCCATCGTTATTCAACTTTCAGATGCATGGATATGCAGATCCAAGCATGTATGGTGCAGGTGCTAACTTTCCATATGGGTTTCCTAACATGTTTCATGGTAGCCATGGTCACGCCCATGGATATTATCACCAGCTCCGTCAAGGTCAGCAAGATTATTATCTGAAGCGGCTGCTTTTGTTTATTGGTTTTTGTGTTCTTCTTGCCCTAATCTGGCAATAGATGAAGGGGTCCATTAGGGCTTATATTACTTCGTGGTGTTTAGTGTATTCAATCTCTAACGTAAGAATTAAAAAAGAAGAATCTGCTGGTACATTGTCAAAAAAGAAAAAAAAGAAAAAAATCTGCTGGTACATTGTCTTGATATCTCATCATTCGTCCTGATTAGTCTTACTGTATGTTGAAATGGACATCGACTTTCTATGCTTAGGCATGGTGAACATCAGCTGACATGCTTATGCAATTGTATTTGAAGGTTTCCATTTATGCATATATATATATATACACACACATTTATTTGCTGACCACCAAATGCAATCAATGCAGAGTGAACAAATCACATGCTCTGTTTTCATTCTCTCTGAGATGATTTATTTGCCTAAGTGGTTTCTCTAGTGCACTTCCTAATGGCTAATGCACCACTTGCGCTATGGTTTCCTTTTTTTTTTTTTCTTTGGCCAATATATCTATGCTGATTCTTGGTGAAGAATTTAGGGTGAAAATGGGGCTGCTGCTGCACATGGTACTTAATGGTGTTTACCTATGGTGTTGTATCCATGTCTATTGTTTCAATTAACAATCTCTTGCTTGCTATTTGTTTCCTTTTCTTAATTCTCAAATTCCAGTTTGGTCTATGTTTGAATTTTAGTCTTGCACCGAGCTTCATGTAACATGTCATGATAGACCATATTTATCAGAAGGGTGCAGAGAAGAAGAGTAGGTGCTTGCTATTGATGCTAGGTATTAACTGAACTTATATATGATGTGCTGTGGCTTGTAGAGCTCAATTGTCTGATGTTATTTTTCTCTTGGAGTATTATGTCAGGGGGGCTATTGTTGGTCCATTGAAATGTTCCCCCCATCCTCATTTGGCAAAGTTAGTATTTAGATCTATGAGTGAAGCATTAGTATATGACAGTTGATACGTTACATATTTTCATTTGTTACTTTGTTATGGTTCATCTTTTGAAGTGTCAATGGTGCAGGATGAGTTACGGATGGCCTCTCTGTCTCAATCACTAGTCGCTAAATTTTGTGGGGACAGGGAGCACAAACCTTGTCCCCAAAATTGAAATAGGAAACATTTCCTCTTACCCCCCTGCCCAAAGTTGAGATTAATGGGGCCTCATATCGTGACATTTCTAGCATTGAAAGAAAGAAGTGAGGTTTCATTTGTTAGGGATAATTATTTGGTTATTATTATTATTTTTATATATAATATTTGGAATTGTTGCATTTTTTTTTATAATAATCTTATCTTTTCTAAGTAAACATTTAGCTAAATTCAAAATTTTAAAACGGCTAAAATTTCAAAAACATTTCTAAAATGTAGTAACAAAACAAAAGGGCTAATTGTCAGGTCACAAATTGATTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGAAAATGATGTTGATAAGACTTTCTTAGAAAAATCTTGGAAATTATGCCATTTTCATTTTTTAAATAAATAAATAAATTTATTATTATTATTAGTTTTGTTAAAATTATTGATATTGATACTTTATGGATACTTTTTGTTATATATATTTTATATTTCCTTGATATTATTACGAAGACTTGGGCATTAGTTTATAGTTTGTGCCAAAAAAGTAAATTATTATAGGCTTGAAAATTATTAAATTAGGGGTGTAAAAGATGTAACTATTTGAACTCTTAGGTCTCTTTAAAATTTTAATAACATTTTGTCTTCAAAAAAAAAAAATTTAATAACATTTTTATAATTGGACAATTGGATGATGGATTGCACAAAGAAAAGTCCCATGCATAAAATATTTAGAGAGATTTTTACAAATAGAAAAAATGTCAAACTATTTACAGAAAATAGCAAACAAAAAAAATGTTGATAGACATTGATAAACTTCTATCAACATCTATTAGTGATAGTAATGATTCATTACTAAGACTTCTATCAAATATCTAAAAATTTTGCTATTTTGTATAAATAATTTCTTTATTTTTATATTTTTAAAAATTAGTATTTTTTCTTTAGAACGGTATAAAAGTTAAAAAGTAGATAAGAAAACAAACATAAAAAAAAAAAAAAAATCCAAATAGAGTTATATTTAGTGAAACTACATTTTTTATAAGCAAAAAGTGTGATAATTAAAGTGACCACTACTAGTCTTGCACATAGGATGTAGACTAAATATCAATGTGTTGTCTAATCTATAGTTGGAAAAATTATTGAAGTGTAGCATGTGGAGAGTTAAGTGGAACTTTACAAATTCCACAACTTTCTTCTAAATTACAAAAGTATTGGGAATAATTTCAATTTTTCTTTTGTTATCTTTTCACAGTATATATTTCTGTATTTAAATTTATCCATTTTTAGCTAACTATTATGTCTTAATTACTTATGTATGGGTAGGTAATTGAATTATTTTTATGCATAAAAATTAAATCAAATAAATTTTATAGATTTTTTTTTTCCATTTTAAAGCATAACTCAAAATATAATTAAGCATATCGATGTGTTTAAGTTTGTATCAATTTAAATCTTGAATTTTAATAATTTTATCAATTTACACCCTCTAGATTTCATTATAAAAATATTGTATGAGACTAACAATTGAATCAATTTAGATTATTTCTTAATTAAGATTGCTTTTGAAAATCGTCGACTTCTTCAAAATTGAAATTGATGGAGCGAAGATTTTGATCGAAGAAATGGATGAATAA

mRNA sequence

ATGGCGAGCGGGTTTGGGGAATCTTCAAGTAGGCAACCTCAAAGCCCTTCTTGTTCCGACAACAATGGTGATGCTAGTAATTTTGAGTGCAATATTTGCCTGGATTTAGCTCAAGATCCTATTGTAACCCTTTGCGGTCATCTTTTCTGTTGGCCATGCCTTTATAAGTGGCTGCACATTCATTCCCATCCCCAGGAATGCCCAGTTTGCAAGGCTATCATAGAAGAGCAAAAATTGGTTCCTTTATATGGTAGGGGAAAGACATCGACTGACCCGAGATCAAAAAGTATTCCTGGAATCAATATTCCTAATCGTCCTGCGGGACAGAGACCTGAAGCAGCAGGTACTACCCCTGAACCAAATCCTTTTCCTCACAATGGGTTTGGATTCATGGGAGGTCTTGGAGGGTTTGCCCCAATGGCATCGACGAGATTGCTTTTGAAAATCGTCGACTTCTTCAAAATTGAAATTGATGGAGCGAAGATTTTGATCGAAGAAATGGATGAATAA

Coding sequence (CDS)

ATGGCGAGCGGGTTTGGGGAATCTTCAAGTAGGCAACCTCAAAGCCCTTCTTGTTCCGACAACAATGGTGATGCTAGTAATTTTGAGTGCAATATTTGCCTGGATTTAGCTCAAGATCCTATTGTAACCCTTTGCGGTCATCTTTTCTGTTGGCCATGCCTTTATAAGTGGCTGCACATTCATTCCCATCCCCAGGAATGCCCAGTTTGCAAGGCTATCATAGAAGAGCAAAAATTGGTTCCTTTATATGGTAGGGGAAAGACATCGACTGACCCGAGATCAAAAAGTATTCCTGGAATCAATATTCCTAATCGTCCTGCGGGACAGAGACCTGAAGCAGCAGGTACTACCCCTGAACCAAATCCTTTTCCTCACAATGGGTTTGGATTCATGGGAGGTCTTGGAGGGTTTGCCCCAATGGCATCGACGAGATTGCTTTTGAAAATCGTCGACTTCTTCAAAATTGAAATTGATGGAGCGAAGATTTTGATCGAAGAAATGGATGAATAA

Protein sequence

MASGFGESSSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEPNPFPHNGFGFMGGLGGFAPMASTRLLLKIVDFFKIEIDGAKILIEEMDE

Homology

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BLAST of HG10004889 vs. NCBI nr
Match: XP_038884023.1 (E3 ubiquitin ligase rnf-5 [Benincasa hispida])

HSP 1 Score: 305.8 bits (782), Expect = 2.3e-79
Identity = 141/144 (97.92%), Postives = 142/144 (98.61%), Query Frame = 0


Query: 1   MASGFGESSSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGES+SRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEP 120
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAG  PEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTR 145
           NPFPHNGFGFMGGLGGFAPMASTR
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTR 144

[Close]

BLAST of HG10004889 vs. NCBI nr
Match: XP_004152714.1 (E3 ubiquitin ligase rnf-5 [Cucumis sativus] >XP_011649602.1 E3 ubiquitin ligase rnf-5 [Cucumis sativus] >KGN62568.1 hypothetical protein Csa_022553 [Cucumis sativus])

HSP 1 Score: 303.9 bits (777), Expect = 8.6e-79
Identity = 140/144 (97.22%), Postives = 141/144 (97.92%), Query Frame = 0


Query: 1   MASGFGESSSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           M SGFGES+SRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MESGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEP 120
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAG  PEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTR 145
           NPFPHNGFGFMGGLGGFAPMASTR
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTR 144

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BLAST of HG10004889 vs. NCBI nr
Match: XP_008444676.1 (PREDICTED: RING finger protein 5 [Cucumis melo] >XP_008444677.1 PREDICTED: RING finger protein 5 [Cucumis melo] >KAA0065261.1 RING finger protein 5 [Cucumis melo var. makuwa])

HSP 1 Score: 302.0 bits (772), Expect = 3.3e-78
Identity = 139/144 (96.53%), Postives = 140/144 (97.22%), Query Frame = 0


Query: 1   MASGFGESSSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASG GES+SRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGLGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEP 120
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSK IPGINIPNRPAGQRPEAAG  PEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKGIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTR 145
           NPFPHNGFGFMGGLGGFAPMASTR
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTR 144

[Close]

BLAST of HG10004889 vs. NCBI nr
Match: KAG7020533.1 (putative galacturonosyltransferase-like 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 301.2 bits (770), Expect = 5.6e-78
Identity = 138/144 (95.83%), Postives = 141/144 (97.92%), Query Frame = 0


Query: 1   MASGFGESSSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGES+SRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEP 120
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPG+NIPNRPAGQRPEAAG  PEP
Sbjct: 61  HSHPQECPVCKAMIEEQKLVPLYGRGKTSTDPRSKSIPGVNIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTR 145
           NPF HNGFGFMGGLGGFAPMASTR
Sbjct: 121 NPFAHNGFGFMGGLGGFAPMASTR 144

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BLAST of HG10004889 vs. NCBI nr
Match: XP_022951148.1 (RING finger protein 5 [Cucurbita moschata] >XP_023537562.1 RING finger protein 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 301.2 bits (770), Expect = 5.6e-78
Identity = 138/144 (95.83%), Postives = 141/144 (97.92%), Query Frame = 0


Query: 1   MASGFGESSSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGES+SRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEP 120
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPG+NIPNRPAGQRPEAAG  PEP
Sbjct: 61  HSHPQECPVCKAMIEEQKLVPLYGRGKTSTDPRSKSIPGVNIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTR 145
           NPF HNGFGFMGGLGGFAPMASTR
Sbjct: 121 NPFAHNGFGFMGGLGGFAPMASTR 144

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BLAST of HG10004889 vs. ExPASy Swiss-Prot
Match: Q09463 (E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.7e-27
Identity = 64/134 (47.76%), Postives = 84/134 (62.69%), Query Frame = 0


Query: 8   SSSRQP-QSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQE 67
           S ++ P + P+ S N  +++ FECNICLD A+D +V+LCGHLFCWPCL +WL    + Q 
Sbjct: 3   SETKAPSEEPTSSSNKDESARFECNICLDAAKDAVVSLCGHLFCWPCLSQWLDTRPNNQV 62

Query: 68  CPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEPNPFPHN 127
           CPVCK+ I+  K+VP+YGRG  S+DPR K      +P RP GQR E     P P  F   
Sbjct: 63  CPVCKSAIDGNKVVPIYGRGGDSSDPRKK------VPPRPKGQRSE-----PPPQSFAGF 122

Query: 128 GFGFMGG-LGGFAP 140
            +G  GG +GG  P
Sbjct: 123 NWGGDGGMMGGGGP 125

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BLAST of HG10004889 vs. ExPASy Swiss-Prot
Match: Q5ZIR9 (E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.3e-26
Identity = 66/130 (50.77%), Postives = 78/130 (60.00%), Query Frame = 0


Query: 9   SSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECP 68
           +S    S    DN    + FECNICLD A+D +++LCGHLFCWPCL++WL    + Q CP
Sbjct: 20  TSGSSSSNGAGDNTNQDNTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCP 79

Query: 69  VCKAIIEEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAAGTTPEPNPFPHNG 128
           VCKA I   K++PLYGRG T   DPR K+      P RP GQRPE     PE N     G
Sbjct: 80  VCKAGISRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-----PE-NRGGFQG 135

Query: 129 FGFMGGLGGF 138
           FGF  G GGF
Sbjct: 140 FGF--GDGGF 135

[Close]

BLAST of HG10004889 vs. ExPASy Swiss-Prot
Match: Q96GF1 (E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.0e-26
Identity = 72/143 (50.35%), Postives = 85/143 (59.44%), Query Frame = 0


Query: 6   GESSSRQPQS-----PSCSDN-----NGDASNFECNICLDLAQDPIVTLCGHLFCWPCLY 65
           G S+S  P++     PS S N      G  S FECNICLD A+D +++LCGHLFCWPCL+
Sbjct: 5   GPSASASPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLH 64

Query: 66  KWLHIHSHPQECPVCKAIIEEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAA 125
           +WL    + Q CPVCKA I   K++PLYGRG T   DPR K+      P RP GQRPE  
Sbjct: 65  QWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-- 124

Query: 126 GTTPEPNPFPHNGFGFMGGLGGF 138
              PE N     GFGF  G GGF
Sbjct: 125 ---PE-NRGGFQGFGF--GDGGF 133

[Close]

BLAST of HG10004889 vs. ExPASy Swiss-Prot
Match: Q91YT2 (E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.0e-26
Identity = 65/124 (52.42%), Postives = 77/124 (62.10%), Query Frame = 0


Query: 15  SPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPVCKAII 74
           S    ++ G  S FECNICLD A+D +++LCGHLFCWPCL++WL    + Q CPVCKA I
Sbjct: 24  SNGTGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGI 83

Query: 75  EEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAAGTTPEPNPFPHNGFGFMGG 134
              K++PLYGRG T   DPR K+      P RP GQRPE     PE N     GFGF  G
Sbjct: 84  SRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-----PE-NRGGFQGFGF--G 133

Query: 135 LGGF 138
            GGF
Sbjct: 144 DGGF 133

[Close]

BLAST of HG10004889 vs. ExPASy Swiss-Prot
Match: Q5RFK9 (E3 ubiquitin-protein ligase RNF185 OS=Pongo abelii OX=9601 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.0e-26
Identity = 72/143 (50.35%), Postives = 85/143 (59.44%), Query Frame = 0


Query: 6   GESSSRQPQS-----PSCSDN-----NGDASNFECNICLDLAQDPIVTLCGHLFCWPCLY 65
           G S+S  P++     PS S N      G  S FECNICLD A+D +++LCGHLFCWPCL+
Sbjct: 5   GPSASSSPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLH 64

Query: 66  KWLHIHSHPQECPVCKAIIEEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAA 125
           +WL    + Q CPVCKA I   K++PLYGRG T   DPR K+      P RP GQRPE  
Sbjct: 65  QWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-- 124

Query: 126 GTTPEPNPFPHNGFGFMGGLGGF 138
              PE N     GFGF  G GGF
Sbjct: 125 ---PE-NRGGFQGFGF--GDGGF 133

[Close]

BLAST of HG10004889 vs. ExPASy TrEMBL
Match: A0A0A0LLD2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G360850 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.2e-79
Identity = 140/144 (97.22%), Postives = 141/144 (97.92%), Query Frame = 0


Query: 1   MASGFGESSSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           M SGFGES+SRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MESGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEP 120
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAG  PEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTR 145
           NPFPHNGFGFMGGLGGFAPMASTR
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTR 144

[Close]

BLAST of HG10004889 vs. ExPASy TrEMBL
Match: A0A1S3BBQ8 (RING finger protein 5 OS=Cucumis melo OX=3656 GN=LOC103487942 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.6e-78
Identity = 139/144 (96.53%), Postives = 140/144 (97.22%), Query Frame = 0


Query: 1   MASGFGESSSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASG GES+SRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGLGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEP 120
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSK IPGINIPNRPAGQRPEAAG  PEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKGIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTR 145
           NPFPHNGFGFMGGLGGFAPMASTR
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTR 144

[Close]

BLAST of HG10004889 vs. ExPASy TrEMBL
Match: A0A5A7VHN4 (RING finger protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G006030 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.6e-78
Identity = 139/144 (96.53%), Postives = 140/144 (97.22%), Query Frame = 0


Query: 1   MASGFGESSSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASG GES+SRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGLGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEP 120
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSK IPGINIPNRPAGQRPEAAG  PEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKGIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTR 145
           NPFPHNGFGFMGGLGGFAPMASTR
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTR 144

[Close]

BLAST of HG10004889 vs. ExPASy TrEMBL
Match: A0A6J1GGW7 (RING finger protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111454080 PE=4 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.7e-78
Identity = 138/144 (95.83%), Postives = 141/144 (97.92%), Query Frame = 0


Query: 1   MASGFGESSSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGES+SRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEP 120
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPG+NIPNRPAGQRPEAAG  PEP
Sbjct: 61  HSHPQECPVCKAMIEEQKLVPLYGRGKTSTDPRSKSIPGVNIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTR 145
           NPF HNGFGFMGGLGGFAPMASTR
Sbjct: 121 NPFAHNGFGFMGGLGGFAPMASTR 144

[Close]

BLAST of HG10004889 vs. ExPASy TrEMBL
Match: A0A6J1CT42 (E3 ubiquitin-protein ligase RNF185 OS=Momordica charantia OX=3673 GN=LOC111014071 PE=4 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 6.0e-78
Identity = 139/144 (96.53%), Postives = 140/144 (97.22%), Query Frame = 0


Query: 1   MASGFGESSSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60
           MASGFGES+SRQPQSPSCSDNN DASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEP 120
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAG  PEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 121 NPFPHNGFGFMGGLGGFAPMASTR 145
           N FPHNGFGFMGGLGGFAPMASTR
Sbjct: 121 NHFPHNGFGFMGGLGGFAPMASTR 144

[Close]

BLAST of HG10004889 vs. TAIR 10
Match: AT1G19310.1 (RING/U-box superfamily protein )

HSP 1 Score: 202.2 bits (513), Expect = 3.3e-52
Identity = 96/134 (71.64%), Postives = 109/134 (81.34%), Query Frame = 0


Query: 16  PSCSD----NNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPVCK 75
           PSCS     NN D+SNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLH+HS  ++CPVCK
Sbjct: 5   PSCSSGNDTNNNDSSNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQSKDCPVCK 64

Query: 76  AIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEPNPFPHNGFGFM 135
           A+IEE +LVPLYGRGK+S DPRSKSIPG+ +PNRP+GQRPE A   P+PN    +  GF 
Sbjct: 65  AVIEEDRLVPLYGRGKSSADPRSKSIPGLEVPNRPSGQRPETA-QPPDPNHGFAHHHGFG 124

Query: 136 GGLGGF-APMASTR 145
           G +GGF APMAS R
Sbjct: 125 GFMGGFAAPMASAR 137

[Close]

BLAST of HG10004889 vs. TAIR 10
Match: AT2G23780.1 (RING/U-box superfamily protein )

HSP 1 Score: 188.7 bits (478), Expect = 3.8e-48
Identity = 89/142 (62.68%), Postives = 104/142 (73.24%), Query Frame = 0


Query: 6   GESSSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQ 65
           GESS+    S S +D N    +FECNIC +LAQDPIVTLCGHLFCWPCLY+WLH HSH Q
Sbjct: 4   GESSTSTSYSDSNNDTNDQGGDFECNICFELAQDPIVTLCGHLFCWPCLYRWLHHHSHSQ 63

Query: 66  ECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEP---NP 125
           ECPVCKA++++ KLVPLYGRGK  TDPRSK  PG+ IPNRP GQRPE A   P+P   + 
Sbjct: 64  ECPVCKAVVQDDKLVPLYGRGKNQTDPRSKRYPGLRIPNRPTGQRPETAAPPPQPEAASN 123

Query: 126 FPHNGFGFMGGLGGFAPMASTR 145
           F + G G MGG+     MA+TR
Sbjct: 124 FFNYGIGLMGGI--MPMMATTR 143

[Close]

BLAST of HG10004889 vs. TAIR 10
Match: AT1G74990.1 (RING/U-box superfamily protein )

HSP 1 Score: 152.9 bits (385), Expect = 2.3e-37
Identity = 70/122 (57.38%), Postives = 85/122 (69.67%), Query Frame = 0


Query: 19  SDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPVCKAIIEEQK 78
           ++ +  ++NF CNICL+LA++PIVTLCGHLFCWPCLYKWLH HS    CPVCKA+++E  
Sbjct: 8   NEEDDASNNFGCNICLELAREPIVTLCGHLFCWPCLYKWLHYHSKSNHCPVCKALVKEDT 67

Query: 79  LVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGTTPEPNPFPHNGFGFMGGLGGFA 138
           LVPLYG GK S+DPRSK   G+ +PNRPA  R E A    E     H+G  F GG   FA
Sbjct: 68  LVPLYGMGKPSSDPRSKLNSGVTVPNRPAATRTETARPRLEQR---HHGSSFFGGHSSFA 126

Query: 139 PM 141
            M
Sbjct: 128 AM 126

[Close]

BLAST of HG10004889 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 109.8 bits (273), Expect = 2.2e-24
Identity = 50/110 (45.45%), Postives = 70/110 (63.64%), Query Frame = 0


Query: 7   ESSSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHS---- 66
           +S   +P + S   ++ D SNF+CNICLD  Q+P+VTLCGHLFCWPC++KWL + S    
Sbjct: 25  KSKKPEPITVSVPSDDTDDSNFDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTS 84

Query: 67  ----HPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAG 109
                 ++CPVCK+ +    LVPLYGRG+ +T    K+    ++P RP G
Sbjct: 85  DEYQRHRQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKN----SVPKRPVG 130

[Close]

BLAST of HG10004889 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 109.8 bits (273), Expect = 2.2e-24
Identity = 50/110 (45.45%), Postives = 70/110 (63.64%), Query Frame = 0


Query: 7   ESSSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHS---- 66
           +S   +P + S   ++ D SNF+CNICLD  Q+P+VTLCGHLFCWPC++KWL + S    
Sbjct: 25  KSKKPEPITVSVPSDDTDDSNFDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTS 84

Query: 67  ----HPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAG 109
                 ++CPVCK+ +    LVPLYGRG+ +T    K+    ++P RP G
Sbjct: 85  DEYQRHRQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKN----SVPKRPVG 130

The following BLAST results are available for this feature:

BLAST of HG10004889 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884023.1	2.3e-79	97.92	E3 ubiquitin ligase rnf-5 [Benincasa hispida]	[more]
XP_004152714.1	8.6e-79	97.22	E3 ubiquitin ligase rnf-5 [Cucumis sativus] >XP_011649602.1 E3 ubiquitin ligase ...	[more]
XP_008444676.1	3.3e-78	96.53	PREDICTED: RING finger protein 5 [Cucumis melo] >XP_008444677.1 PREDICTED: RING ...	[more]
KAG7020533.1	5.6e-78	95.83	putative galacturonosyltransferase-like 2, partial [Cucurbita argyrosperma subsp...	[more]
XP_022951148.1	5.6e-78	95.83	RING finger protein 5 [Cucurbita moschata] >XP_023537562.1 RING finger protein 5...	[more]

BLAST of HG10004889 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q09463	2.7e-27	47.76	E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1	[more]
Q5ZIR9	2.3e-26	50.77	E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1	[more]
Q96GF1	3.0e-26	50.35	E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1	[more]
Q91YT2	3.0e-26	52.42	E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1	[more]
Q5RFK9	3.0e-26	50.35	E3 ubiquitin-protein ligase RNF185 OS=Pongo abelii OX=9601 GN=RNF185 PE=2 SV=1	[more]

BLAST of HG10004889 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LLD2	4.2e-79	97.22	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G360850 P...	[more]
A0A1S3BBQ8	1.6e-78	96.53	RING finger protein 5 OS=Cucumis melo OX=3656 GN=LOC103487942 PE=4 SV=1	[more]
A0A5A7VHN4	1.6e-78	96.53	RING finger protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82...	[more]
A0A6J1GGW7	2.7e-78	95.83	RING finger protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111454080 PE=4 SV=1	[more]
A0A6J1CT42	6.0e-78	96.53	E3 ubiquitin-protein ligase RNF185 OS=Momordica charantia OX=3673 GN=LOC11101407...	[more]

BLAST of HG10004889 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G19310.1	3.3e-52	71.64	RING/U-box superfamily protein	[more]
AT2G23780.1	3.8e-48	62.68	RING/U-box superfamily protein	[more]
AT1G74990.1	2.3e-37	57.38	RING/U-box superfamily protein	[more]
AT4G03510.1	2.2e-24	45.45	RING membrane-anchor 1	[more]
AT4G03510.2	2.2e-24	45.45	RING membrane-anchor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 30..70 e-value: 6.4E-8 score: 42.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 30..71 score: 13.256433
None	No IPR available	PFAM	PF13923	zf-C3HC4_2	coord: 29..70 e-value: 1.1E-8 score: 34.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 84..122
None	No IPR available	PANTHER	PTHR12313:SF39	OS12G0636000 PROTEIN	coord: 2..145
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 28..72 e-value: 3.6546E-30 score: 102.382
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 26..83
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 6..96 e-value: 3.0E-17 score: 64.8
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 2..145
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 45..54

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10004889.1	HG10004889.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071712	ER-associated misfolded protein catabolic process
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0044390	ubiquitin-like protein conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity