HG10004875 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10004875 vs. NCBI nr
Match: XP_038885660.1 (uncharacterized membrane protein At1g75140-like [Benincasa hispida])

HSP 1 Score: 584.3 bits (1505), Expect = 6.9e-163
Identity = 309/423 (73.05%), Postives = 327/423 (77.30%), Query Frame = 0

Query: 1   MHRIWEGSNGEELNSIFMEHVVEFVATGSQEDESLISILEVHHVERTKYILSPDVRGKIK 60
           MHRIWEGSNGEELN IFMEHVVEF  T SQEDES ISILEVHHV  T+YILS D RG IK
Sbjct: 222 MHRIWEGSNGEELNLIFMEHVVEFADTDSQEDESPISILEVHHVGWTRYILSSDFRGVIK 281

Query: 61  VFREDGTVYGSVMPMSRPKAFLKQRLLFLTQSGTGSLDLRSMRRRESECEGLNYSLAQHY 120
           VF+EDGTVYGSVMPMSRP AFLKQRLLFLT+SG GSLDLRSM+ RESECEGLNYSLA++Y
Sbjct: 282 VFKEDGTVYGSVMPMSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNY 341

Query: 121 VFDATEQSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS 180
           VFDATE+SKAYG+TSDGDLIHVLLLGDIMNFKCR+RSKRKFELD PLVFQAIKGYLLVIS
Sbjct: 342 VFDATERSKAYGFTSDGDLIHVLLLGDIMNFKCRIRSKRKFELDGPLVFQAIKGYLLVIS 401

Query: 181 NEKVHIFNASSQHYVRVGAPRLLFSAVLYEIRPSVLNYQNLDLEFEGNFIPLISSDREKL 240
           NEKVHIFN SSQHYVRVGAPRLLFSA L EI  S LNYQNLDLE EGNFIPLISSDREKL
Sbjct: 402 NEKVHIFNVSSQHYVRVGAPRLLFSAGLDEIMSSFLNYQNLDLESEGNFIPLISSDREKL 461

Query: 241 VVLGLGGGCLGI------------------------------------------------ 300
           VVLGLGGG +G+                                                
Sbjct: 462 VVLGLGGGYVGMYRSNLPVFKGEFNTMLWTSPVLFFILFLFGAWHLFAKKKEALTSWGPD 521

Query: 301 -------------------ERASFIDTPSRSTDMMDLRGGGGLRGPPWRYGSPIGYPGGA 357
                              ERASFID PSR+TDMMDLRGG GLRGPP RYGSP GYPGGA
Sbjct: 522 DSFTATSPTTGAPLGTGSSERASFIDPPSRTTDMMDLRGGAGLRGPPRRYGSPTGYPGGA 581

BLAST of HG10004875 vs. NCBI nr
Match: XP_038885478.1 (uncharacterized membrane protein At1g75140-like [Benincasa hispida])

HSP 1 Score: 582.0 bits (1499), Expect = 3.4e-162
Identity = 307/423 (72.58%), Postives = 328/423 (77.54%), Query Frame = 0

Query: 1   MHRIWEGSNGEELNSIFMEHVVEFVATGSQEDESLISILEVHHVERTKYILSPDVRGKIK 60
           MHRIWEG NGEELNSIFMEHVVEFVAT S+ED+S ISILEVHHV RT+YILS D RGKIK
Sbjct: 234 MHRIWEGLNGEELNSIFMEHVVEFVATDSREDDSPISILEVHHVGRTRYILSSDFRGKIK 293

Query: 61  VFREDGTVYGSVMPMSRPKAFLKQRLLFLTQSGTGSLDLRSMRRRESECEGLNYSLAQHY 120
           VF+EDGTVYGSV  MSRP AFLKQRLLFLT+SG GSLDLRSM+ RE+ CEGLNYSLA++Y
Sbjct: 294 VFKEDGTVYGSVTLMSRPIAFLKQRLLFLTESGAGSLDLRSMKLREAGCEGLNYSLARNY 353

Query: 121 VFDATEQSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS 180
           VFDATE+SKAYG+TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS
Sbjct: 354 VFDATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS 413

Query: 181 NEKVHIFNASSQHYVRVGAPRLLFSAVLYEIRPSVLNYQNLDLEFEGNFIPLISSDREKL 240
           NEKVH+FN SSQHYVRV APRLLFSA L EIR S LNYQNL+LE EG FIPLISSD EKL
Sbjct: 414 NEKVHVFNVSSQHYVRVAAPRLLFSAGLDEIRSSFLNYQNLNLESEGKFIPLISSDHEKL 473

Query: 241 VVLGLGGGCLGI------------------------------------------------ 300
           VVLGLGGG +G+                                                
Sbjct: 474 VVLGLGGGYVGMYRSNLPIFKGEFNSMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPD 533

Query: 301 -------------------ERASFIDTPSRSTDMMDLRGGGGLRGPPWRYGSPIGYPGGA 357
                              ERASFIDTPSRSTDMMDLRGGGG+RGPP  YGSP GYPGGA
Sbjct: 534 DPFTATSPTTGVPLGTGSSERASFIDTPSRSTDMMDLRGGGGIRGPPRHYGSPTGYPGGA 593

BLAST of HG10004875 vs. NCBI nr
Match: XP_008444699.1 (PREDICTED: uncharacterized membrane protein At1g75140-like [Cucumis melo])

HSP 1 Score: 571.6 bits (1472), Expect = 4.7e-159
Identity = 304/426 (71.36%), Postives = 328/426 (77.00%), Query Frame = 0

Query: 1   MHRIWEGSNGEELNSIFMEHVVEFVATGSQEDE--SLISILEVHHVERTKYILSPDVRGK 60
           MHRIWEGSNGE+LN IFME VVEFVAT S+EDE  S IS+LE+HHV RT+YILS D+RGK
Sbjct: 228 MHRIWEGSNGEDLNLIFMERVVEFVATDSREDESQSQISLLELHHVGRTRYILSSDLRGK 287

Query: 61  IKVFREDGTVYGSVMPMSRPKAFLKQRLLFLTQSGTGSLDLRSMRRRESECEGLNYSLAQ 120
           IKVF+EDGTVYGSVMP SRP AFLKQRLLFLT+SG GSLDLRSM+ RESECEGLN+SL +
Sbjct: 288 IKVFKEDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLVR 347

Query: 121 HYVFDATEQSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLV 180
           +YVFDATE+SKAYG+TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPL FQ IKGYLLV
Sbjct: 348 NYVFDATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLV 407

Query: 181 ISNEKVHIFNASSQHYVRVGAPRLLFSAVLYEIRPSVLNYQNLDLEFEGNF-IPLISSDR 240
            SNEKVH+FN SS HYVRVGAPRLLFSA L EI+ S LNYQNLDLE E NF IPLISSDR
Sbjct: 408 TSNEKVHVFNVSSHHYVRVGAPRLLFSAGLDEIKSSFLNYQNLDLESEANFYIPLISSDR 467

Query: 241 EKLVVLGLGGGCLGI--------------------------------------------- 300
           EKLVVLGLGGG +G+                                             
Sbjct: 468 EKLVVLGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSW 527

Query: 301 ----------------------ERASFIDTPSRSTDMMDLRGGGGLRGPPWRYGSPIGYP 357
                                 ERASFIDTPSRSTD+MDLRGGGGLRGP  RYGSP GYP
Sbjct: 528 GPDDPFTATSPTTGAPMGTGSSERASFIDTPSRSTDVMDLRGGGGLRGPARRYGSPTGYP 587

BLAST of HG10004875 vs. NCBI nr
Match: XP_011649613.2 (uncharacterized membrane protein At1g75140 [Cucumis sativus] >KAE8652103.1 hypothetical protein Csa_022548 [Cucumis sativus])

HSP 1 Score: 571.2 bits (1471), Expect = 6.1e-159
Identity = 301/423 (71.16%), Postives = 322/423 (76.12%), Query Frame = 0

Query: 1   MHRIWEGSNGEELNSIFMEHVVEFVATGSQEDESLISILEVHHVERTKYILSPDVRGKIK 60
           MHRIWEGSNGE+LN IFMEHVVEFVAT S+EDES IS+LE+HHV RT+YILS D  GKIK
Sbjct: 231 MHRIWEGSNGEDLNLIFMEHVVEFVATNSREDESQISLLELHHVGRTRYILSSDFGGKIK 290

Query: 61  VFREDGTVYGSVMPMSRPKAFLKQRLLFLTQSGTGSLDLRSMRRRESECEGLNYSLAQHY 120
           VFREDGTVYGSVMP SRP AFLKQRLLFLT+SG GSLDLRSM+ RESECEGLN+SLA++Y
Sbjct: 291 VFREDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNY 350

Query: 121 VFDATEQSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS 180
           VFDA E+SKAYG TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPL FQ IKGYLLV S
Sbjct: 351 VFDAMERSKAYGVTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTS 410

Query: 181 NEKVHIFNASSQHYVRVGAPRLLFSAVLYEIRPSVLNYQNLDLEFEGNFIPLISSDREKL 240
           NEKVH+FN SSQHYVRVGAPRLLFSA L EI+ S LNYQN DL  E NFIPLISSD EKL
Sbjct: 411 NEKVHVFNVSSQHYVRVGAPRLLFSAGLDEIKSSFLNYQNSDLVSEANFIPLISSDHEKL 470

Query: 241 VVLGLGGGCLGI------------------------------------------------ 300
           VVLGLGGG +G+                                                
Sbjct: 471 VVLGLGGGYVGMYRSNLPIFKGEFNTMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPD 530

Query: 301 -------------------ERASFIDTPSRSTDMMDLRGGGGLRGPPWRYGSPIGYPGGA 357
                              ERASFIDTPSRSTD+MDLR  GGLRGPP RYGSP GYP GA
Sbjct: 531 DPFTATSPTTGAPMGTGSSERASFIDTPSRSTDVMDLRSAGGLRGPPRRYGSPTGYPAGA 590

BLAST of HG10004875 vs. NCBI nr
Match: XP_022144258.1 (uncharacterized membrane protein At1g75140-like [Momordica charantia])

HSP 1 Score: 570.5 bits (1469), Expect = 1.0e-158
Identity = 303/423 (71.63%), Postives = 324/423 (76.60%), Query Frame = 0

Query: 1   MHRIWEGSNGEELNSIFMEHVVEFVATGSQEDESLISILEVHHVERTKYILSPDVRGKIK 60
           MHRIWE SNGEE +S+FMEHVVEFVAT  +EDES ISILEVHHV RT+YILS DVRGKIK
Sbjct: 235 MHRIWERSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIK 294

Query: 61  VFREDGTVYGSVMPMSRPKAFLKQRLLFLTQSGTGSLDLRSMRRRESECEGLNYSLAQHY 120
           VFRE+GTVYGSVMP S+P AFLKQRLLFLT+SG GSLDLRSM+ RESECEGLNYSLA++Y
Sbjct: 295 VFRENGTVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNY 354

Query: 121 VFDATEQSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS 180
           VFDATE+SKAYG+TS GDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS
Sbjct: 355 VFDATERSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS 414

Query: 181 NEKVHIFNASSQHYVRVGAPRLLFSAVLYEIRPSVLNYQNLDLEFEGNFIPLISSDREKL 240
           NEKVHIFN SSQHY RVGAPR LFSA + EIR S LNYQNLDLE EG F+PLISSDREKL
Sbjct: 415 NEKVHIFNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKL 474

Query: 241 VVLGLGGGCLGI------------------------------------------------ 300
           VVLGLGGG +G+                                                
Sbjct: 475 VVLGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPD 534

Query: 301 -------------------ERASFIDTPSRSTDMMDLRGGGGLRGPPWRYGSPIGYPGGA 357
                              ER+SFIDTPSRSTDMMDLRGG GLRGPP RYGSP GYPGGA
Sbjct: 535 DPFTATSPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGA 594

BLAST of HG10004875 vs. ExPASy Swiss-Prot
Match: Q9FRK5 (Uncharacterized membrane protein At1g75140 OS=Arabidopsis thaliana OX=3702 GN=At1g75140 PE=1 SV=3)

HSP 1 Score: 325.9 bits (834), Expect = 5.8e-88
Identity = 195/427 (45.67%), Postives = 258/427 (60.42%), Query Frame = 0

Query: 1   MHRIWEGSNGEELNSIFMEHVVEFVATGSQEDESLISILEVHHVERTKYILSPDVRGKIK 60
           +HR+ EGS GE+LNS  ME+V +F  T   ED   +++LEVHHV R +YIL+ D+ GK+ 
Sbjct: 206 LHRLREGSIGEDLNSAVMENVGKFDGT---EDGLQVTLLEVHHVGRVRYILATDLSGKLT 265

Query: 61  VFREDGTVYGSVMPMSRPKAFLKQRLLFLTQSGTGSLDLRSMRRRESECEGLNYSLAQHY 120
           VF E+ TVYGSV P SRP  FLKQRLLFLT++G GSLDLRSM+ RESECEGLN+SLA+ Y
Sbjct: 266 VFTENRTVYGSVSPTSRPLVFLKQRLLFLTETGAGSLDLRSMKIRESECEGLNHSLARSY 325

Query: 121 VFDATEQSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS 180
           VFDA+E++KAYG+TS+G++IHVLLLGDIMNFKCRVRSK+K +++EP+  QAIKGYLL+++
Sbjct: 326 VFDASERAKAYGFTSEGEIIHVLLLGDIMNFKCRVRSKKKVQMEEPVALQAIKGYLLIVN 385

Query: 181 NEKVHIFNASSQHYVRVGAPRLLFSAVLYEIRPSVLNY----QNLDLEFEGNFIPLISSD 240
            EKV ++N S+QHYVR   PRLLF A L +IR + L++    +  D +      PLI+SD
Sbjct: 386 QEKVFVYNVSTQHYVRTTGPRLLFPAALEDIRSTFLSHRESTKTTDHQKLEKVTPLIASD 445

Query: 241 REKLVVLGLGGGCLGIER------------------------------------------ 300
           REKL+V+GLG G +   +                                          
Sbjct: 446 REKLLVMGLGDGYVATYKSKLPISKAEFNTMLWSSPVFFFILFLFGAWHFFSKKKESLTA 505

Query: 301 -----------------------ASFIDTPSRSTDMMDLRGGGGLRGPPWRYGSPIGY-P 356
                                  ++F ++  R+ D MDLR          RY SP  Y P
Sbjct: 506 WGPDDPFSSTTMSSSSTTTAQNSSAFSESTRRNDDHMDLRR---------RYVSPSRYPP 565

BLAST of HG10004875 vs. ExPASy TrEMBL
Match: A0A1S3BAX2 (uncharacterized membrane protein At1g75140-like OS=Cucumis melo OX=3656 GN=LOC103487958 PE=4 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 2.3e-159
Identity = 304/426 (71.36%), Postives = 328/426 (77.00%), Query Frame = 0

Query: 1   MHRIWEGSNGEELNSIFMEHVVEFVATGSQEDE--SLISILEVHHVERTKYILSPDVRGK 60
           MHRIWEGSNGE+LN IFME VVEFVAT S+EDE  S IS+LE+HHV RT+YILS D+RGK
Sbjct: 228 MHRIWEGSNGEDLNLIFMERVVEFVATDSREDESQSQISLLELHHVGRTRYILSSDLRGK 287

Query: 61  IKVFREDGTVYGSVMPMSRPKAFLKQRLLFLTQSGTGSLDLRSMRRRESECEGLNYSLAQ 120
           IKVF+EDGTVYGSVMP SRP AFLKQRLLFLT+SG GSLDLRSM+ RESECEGLN+SL +
Sbjct: 288 IKVFKEDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLVR 347

Query: 121 HYVFDATEQSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLV 180
           +YVFDATE+SKAYG+TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPL FQ IKGYLLV
Sbjct: 348 NYVFDATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLV 407

Query: 181 ISNEKVHIFNASSQHYVRVGAPRLLFSAVLYEIRPSVLNYQNLDLEFEGNF-IPLISSDR 240
            SNEKVH+FN SS HYVRVGAPRLLFSA L EI+ S LNYQNLDLE E NF IPLISSDR
Sbjct: 408 TSNEKVHVFNVSSHHYVRVGAPRLLFSAGLDEIKSSFLNYQNLDLESEANFYIPLISSDR 467

Query: 241 EKLVVLGLGGGCLGI--------------------------------------------- 300
           EKLVVLGLGGG +G+                                             
Sbjct: 468 EKLVVLGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSW 527

Query: 301 ----------------------ERASFIDTPSRSTDMMDLRGGGGLRGPPWRYGSPIGYP 357
                                 ERASFIDTPSRSTD+MDLRGGGGLRGP  RYGSP GYP
Sbjct: 528 GPDDPFTATSPTTGAPMGTGSSERASFIDTPSRSTDVMDLRGGGGLRGPARRYGSPTGYP 587

BLAST of HG10004875 vs. ExPASy TrEMBL
Match: A0A0A0LNK9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361500 PE=4 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 2.9e-159
Identity = 301/423 (71.16%), Postives = 322/423 (76.12%), Query Frame = 0

Query: 1   MHRIWEGSNGEELNSIFMEHVVEFVATGSQEDESLISILEVHHVERTKYILSPDVRGKIK 60
           MHRIWEGSNGE+LN IFMEHVVEFVAT S+EDES IS+LE+HHV RT+YILS D  GKIK
Sbjct: 231 MHRIWEGSNGEDLNLIFMEHVVEFVATNSREDESQISLLELHHVGRTRYILSSDFGGKIK 290

Query: 61  VFREDGTVYGSVMPMSRPKAFLKQRLLFLTQSGTGSLDLRSMRRRESECEGLNYSLAQHY 120
           VFREDGTVYGSVMP SRP AFLKQRLLFLT+SG GSLDLRSM+ RESECEGLN+SLA++Y
Sbjct: 291 VFREDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNY 350

Query: 121 VFDATEQSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS 180
           VFDA E+SKAYG TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPL FQ IKGYLLV S
Sbjct: 351 VFDAMERSKAYGVTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTS 410

Query: 181 NEKVHIFNASSQHYVRVGAPRLLFSAVLYEIRPSVLNYQNLDLEFEGNFIPLISSDREKL 240
           NEKVH+FN SSQHYVRVGAPRLLFSA L EI+ S LNYQN DL  E NFIPLISSD EKL
Sbjct: 411 NEKVHVFNVSSQHYVRVGAPRLLFSAGLDEIKSSFLNYQNSDLVSEANFIPLISSDHEKL 470

Query: 241 VVLGLGGGCLGI------------------------------------------------ 300
           VVLGLGGG +G+                                                
Sbjct: 471 VVLGLGGGYVGMYRSNLPIFKGEFNTMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPD 530

Query: 301 -------------------ERASFIDTPSRSTDMMDLRGGGGLRGPPWRYGSPIGYPGGA 357
                              ERASFIDTPSRSTD+MDLR  GGLRGPP RYGSP GYP GA
Sbjct: 531 DPFTATSPTTGAPMGTGSSERASFIDTPSRSTDVMDLRSAGGLRGPPRRYGSPTGYPAGA 590

BLAST of HG10004875 vs. ExPASy TrEMBL
Match: A0A6J1CSU7 (uncharacterized membrane protein At1g75140-like OS=Momordica charantia OX=3673 GN=LOC111013986 PE=4 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 5.0e-159
Identity = 303/423 (71.63%), Postives = 324/423 (76.60%), Query Frame = 0

Query: 1   MHRIWEGSNGEELNSIFMEHVVEFVATGSQEDESLISILEVHHVERTKYILSPDVRGKIK 60
           MHRIWE SNGEE +S+FMEHVVEFVAT  +EDES ISILEVHHV RT+YILS DVRGKIK
Sbjct: 235 MHRIWERSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGKIK 294

Query: 61  VFREDGTVYGSVMPMSRPKAFLKQRLLFLTQSGTGSLDLRSMRRRESECEGLNYSLAQHY 120
           VFRE+GTVYGSVMP S+P AFLKQRLLFLT+SG GSLDLRSM+ RESECEGLNYSLA++Y
Sbjct: 295 VFRENGTVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNY 354

Query: 121 VFDATEQSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS 180
           VFDATE+SKAYG+TS GDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS
Sbjct: 355 VFDATERSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS 414

Query: 181 NEKVHIFNASSQHYVRVGAPRLLFSAVLYEIRPSVLNYQNLDLEFEGNFIPLISSDREKL 240
           NEKVHIFN SSQHY RVGAPR LFSA + EIR S LNYQNLDLE EG F+PLISSDREKL
Sbjct: 415 NEKVHIFNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDREKL 474

Query: 241 VVLGLGGGCLGI------------------------------------------------ 300
           VVLGLGGG +G+                                                
Sbjct: 475 VVLGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPD 534

Query: 301 -------------------ERASFIDTPSRSTDMMDLRGGGGLRGPPWRYGSPIGYPGGA 357
                              ER+SFIDTPSRSTDMMDLRGG GLRGPP RYGSP GYPGGA
Sbjct: 535 DPFTATSPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPGGA 594

BLAST of HG10004875 vs. ExPASy TrEMBL
Match: A0A6J1HC37 (uncharacterized membrane protein At1g75140-like OS=Cucurbita moschata OX=3662 GN=LOC111462650 PE=4 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 6.6e-159
Identity = 301/437 (68.88%), Postives = 326/437 (74.60%), Query Frame = 0

Query: 1   MHRIWEGSNGEELNSIFMEHVVEFVATGSQEDESLISILEVHHVERTKYILSPDVRGKIK 60
           MHRIWEGSNGEE NSIFMEHV+EFV T  +E++S ISILEVHHV RT+YILS DV+GKIK
Sbjct: 231 MHRIWEGSNGEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIK 290

Query: 61  VFREDGTVYGSVMPMSRPKAFLKQRLLFLTQSGTGSLDLRSMRRRESECEGLNYSLAQHY 120
           VFREDGTVYGSVMP SRP AFLKQRLLFLT++G GSLDLRSM+ RESECEGLNYSLA++Y
Sbjct: 291 VFREDGTVYGSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNY 350

Query: 121 VFDATEQSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS 180
           VFDATE+SKAYG+TSDGDLIHV+LLGDIMNFKCRVRSK+KFELDEPL FQA+KGYLLVI 
Sbjct: 351 VFDATERSKAYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIG 410

Query: 181 NEKVHIFNASSQHYVRVGAPRLLFSAVLYEIRPSVLNYQNLDLEFEGNFIPLISSDREKL 240
           +EKVH+FN SSQHYVRVGAPRLLFSA L EIR S LN QNLDLE +G FIPLISSDREKL
Sbjct: 411 SEKVHVFNVSSQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKL 470

Query: 241 VVLGLGGGCLGIERA--------------------------------------------- 300
           VVLGLGGG +G+ R+                                             
Sbjct: 471 VVLGLGGGYIGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPD 530

Query: 301 ------------------------------------SFIDTPSRSTDMMDLRGGGGLRGP 357
                                               SFIDTPSRSTDMMDLRGG GLRGP
Sbjct: 531 DPFTATSPTTGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGP 590

BLAST of HG10004875 vs. ExPASy TrEMBL
Match: A0A6J1GHT9 (uncharacterized membrane protein At1g75140-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454317 PE=4 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 9.5e-158
Identity = 303/424 (71.46%), Postives = 325/424 (76.65%), Query Frame = 0

Query: 1   MHRIWEGSNGEELNSIFMEHVVEFVATGSQEDESLISILEVHHVERTKYILSPDVRGKIK 60
           MHRIWE SNGE+LNS+FMEHVVEFVA    EDES ISILEVH+V R +YILS +VRGKIK
Sbjct: 215 MHRIWERSNGEDLNSVFMEHVVEFVA----EDESPISILEVHYVGRIRYILSSNVRGKIK 274

Query: 61  VFREDGTVYGSVMPMSRPKAFLKQRLLFLTQSGTGSLDLRSMRRRESECEGLNYSLAQHY 120
           VFREDGTVYGSVMPMSRP  FLKQRLLFLT+SG GSLDLRSM+ RESECEGLN+SLA++Y
Sbjct: 275 VFREDGTVYGSVMPMSRPIVFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNY 334

Query: 121 VFDATEQSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS 180
           VFDA E+SKAYG+TSDGDLIHVLLLGDI+NFKCRVRSKRKFELDEPL+FQAIKGYLLVIS
Sbjct: 335 VFDAVERSKAYGFTSDGDLIHVLLLGDILNFKCRVRSKRKFELDEPLIFQAIKGYLLVIS 394

Query: 181 NEKVHIFNASSQHYVRVGAPRLLFSAVLYEIRPSVLNYQNLDLEFEGNFIPLISSDREKL 240
           NEKVHI+N SSQHYVRVGAPR LFSA L EIR S LNYQN+D E  G FIPLISSD EKL
Sbjct: 395 NEKVHIYNVSSQHYVRVGAPRHLFSAGLDEIRSSFLNYQNMDSESGGKFIPLISSDHEKL 454

Query: 241 VVLGLGGGCLGI------------------------------------------------ 300
           VVLGLGGG +G+                                                
Sbjct: 455 VVLGLGGGYVGLYRSNLPIFKGEFNTVLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPD 514

Query: 301 -------------------ERASFIDTPSRSTDMMDLR-GGGGLRGPPWRYGSPIGYPGG 357
                              ERASFID PSRSTDMMDLR GGGGLRGPP RYGSP GYPGG
Sbjct: 515 DPFTATSPTTGAPLGTGSSERASFIDAPSRSTDMMDLRGGGGGLRGPPRRYGSPTGYPGG 574

BLAST of HG10004875 vs. TAIR 10
Match: AT1G75140.1 (unknown protein; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G19370.1); Has 51 Blast hits to 49 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink). )

HSP 1 Score: 325.9 bits (834), Expect = 4.1e-89
Identity = 195/427 (45.67%), Postives = 258/427 (60.42%), Query Frame = 0

Query: 1   MHRIWEGSNGEELNSIFMEHVVEFVATGSQEDESLISILEVHHVERTKYILSPDVRGKIK 60
           +HR+ EGS GE+LNS  ME+V +F  T   ED   +++LEVHHV R +YIL+ D+ GK+ 
Sbjct: 206 LHRLREGSIGEDLNSAVMENVGKFDGT---EDGLQVTLLEVHHVGRVRYILATDLSGKLT 265

Query: 61  VFREDGTVYGSVMPMSRPKAFLKQRLLFLTQSGTGSLDLRSMRRRESECEGLNYSLAQHY 120
           VF E+ TVYGSV P SRP  FLKQRLLFLT++G GSLDLRSM+ RESECEGLN+SLA+ Y
Sbjct: 266 VFTENRTVYGSVSPTSRPLVFLKQRLLFLTETGAGSLDLRSMKIRESECEGLNHSLARSY 325

Query: 121 VFDATEQSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVIS 180
           VFDA+E++KAYG+TS+G++IHVLLLGDIMNFKCRVRSK+K +++EP+  QAIKGYLL+++
Sbjct: 326 VFDASERAKAYGFTSEGEIIHVLLLGDIMNFKCRVRSKKKVQMEEPVALQAIKGYLLIVN 385

Query: 181 NEKVHIFNASSQHYVRVGAPRLLFSAVLYEIRPSVLNY----QNLDLEFEGNFIPLISSD 240
            EKV ++N S+QHYVR   PRLLF A L +IR + L++    +  D +      PLI+SD
Sbjct: 386 QEKVFVYNVSTQHYVRTTGPRLLFPAALEDIRSTFLSHRESTKTTDHQKLEKVTPLIASD 445

Query: 241 REKLVVLGLGGGCLGIER------------------------------------------ 300
           REKL+V+GLG G +   +                                          
Sbjct: 446 REKLLVMGLGDGYVATYKSKLPISKAEFNTMLWSSPVFFFILFLFGAWHFFSKKKESLTA 505

Query: 301 -----------------------ASFIDTPSRSTDMMDLRGGGGLRGPPWRYGSPIGY-P 356
                                  ++F ++  R+ D MDLR          RY SP  Y P
Sbjct: 506 WGPDDPFSSTTMSSSSTTTAQNSSAFSESTRRNDDHMDLRR---------RYVSPSRYPP 565

BLAST of HG10004875 vs. TAIR 10
Match: AT1G19370.1 (unknown protein; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G75140.1); Has 45 Blast hits to 43 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 44; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 303.1 bits (775), Expect = 2.9e-82
Identity = 185/418 (44.26%), Postives = 243/418 (58.13%), Query Frame = 0

Query: 1   MHRIWEGSNGEE--LNSIFMEHVVEFVATGSQEDESLISILEVHHVERTKYILSPDVRGK 60
           +HRI EGSNGE+   NS+ MEHV +F    S +    +++LEVHHV R +YIL+ D+ GK
Sbjct: 207 LHRIHEGSNGEDWNSNSVSMEHVGKFDVDDSADP---VTLLEVHHVGRVRYILATDLSGK 266

Query: 61  IKVFREDGTVYGSVMPMSRPKAFLKQRLLFLTQSGTGSLDLRSMRRRESECEGLNYSLAQ 120
           + V  E+ TVYGSV+P SRP  FLKQRLLFLT+SG GSLDLRSM+ RE+ECEGLN+SLA+
Sbjct: 267 LTVLTENRTVYGSVIPSSRPLVFLKQRLLFLTESGAGSLDLRSMKIRETECEGLNHSLAR 326

Query: 121 HYVFDATEQSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLV 180
            YVFDA E+SKAYG+TS+G++IHVLL GDIMNFKCRVRSK+KF+++EP+  Q+IKGYLLV
Sbjct: 327 TYVFDAAERSKAYGFTSEGEIIHVLLHGDIMNFKCRVRSKKKFQMEEPVALQSIKGYLLV 386

Query: 181 ISNEKVHIFNASSQHYVRVGAPRLLFSAVLYEIRPSVLNYQNLDLEFEG--NFIPLISSD 240
           I+ EKV  FN S+QHYVR   PRLLFSA L EIR + L+++             PLI+SD
Sbjct: 387 INEEKVFAFNVSTQHYVRTAGPRLLFSAGLEEIRSAFLSHRESSSRTTTVVKTRPLIASD 446

Query: 241 REKLVVLGLGGGCLGIERA----------------------------------------- 300
           RE L+V+GL  G   + ++                                         
Sbjct: 447 RENLLVIGLENGYFAVYKSKLPTLKGDFNTMLWSSPVFFFILFLFGAWHFFAKKKESLTA 506

Query: 301 --------------------SFIDTPSRSTDMMDLRGGGGLRGPPWRYGSPIGYPGGATG 350
                               +F +   R+ D+MDLR          RY         A G
Sbjct: 507 WGPDDPFTPTGAQNSSAKEPTFTEPSRRNDDLMDLRR---------RY---------AGG 566