HG10004825 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10004825
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionCytokinin dehydrogenase
LocationChr08: 20737245 .. 20742906 (+)
RNA-Seq ExpressionHG10004825
SyntenyHG10004825
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAACACTGCCACCGCCGCCGTCCGATTACCTTCGCCGCCGTGAAAAGTTTCATTAATAATTCAACAATGATGACCACTAAGCTTCTCTTGGCCTTTGCCTTCTGCCGTTTGATAGCCACCGTGGGACTCGACATGGGTCCGGCGGCGGAGCTTCCCCGTCTCGGTATCGACGGCCTTCTCACCGTCGACCCTTCCGATATCGAAACCGCTTCTATCGATTTCGGCCTCATGACTCGAAATCCGCCTCTCGCCGTCTTGCACCCAGCCTCCGCCGATGACGTCGCCAAAGTGATCCGGACGGCGGTTGCGGCGGCGGATACGGCGGAGTATGGTGGGTTTACGGTGTCGGCGAGAGGGCACGGGCATTCGATAAACGGGCAGGCGCAGACGGGGAATGGGGTGGTGATTGAGATGAGTGGCGGCGGAAGACGGCACCGGCGCGGCGGCGGGAGGGGTATTTCGCCGCCTCCGCCGCCGCATGTGGTGTCGGAGAAAGAGAGATTTGTAGACGTATGGGGCGGAGAGCTGTGGATTGATGTACTGAAATCGACGTTGGGATATGGGTTGGCGCCGAGATCGTGGACGGATTATTTGTATTTGACGGTCGGCGGAACGCTGTCGAACGGCGGAATCAGCGGCCAAGCTTTCAACCATGGCCCTCAGATCAGCAATGTCCATGAACTTGACGTCGTCACAGGTCCATTTTTTTTTTTCTTACCAACAAAAATGCAAATGCAATTATTCAAAATTAAATCTTTAATTTATTATATACTTTTTTCTACTCGAAAAAAAAAATCGTGAGATTAAACAGTTTTATGGTCGTTTTTGTTTAGTTCAAAATTTTCATACAAAATTAGTAGTTTATGTTTTATCTCGAACTATTTATTATGCTTATTTTCTTACCTCTTTTAAACTAGATAACAAAATAAATAAATCTGTATGTGAAAAGTTTTGATAATAATCTTTTTATTTAAAAAAAAAATTACACTACTCTCCCCTATAAATTTATTTGTCATGTTATCTATTTTTTAATTATTTAATTATATTATGAAAACTTAACAAATCAGTTTATTTAAATCTGGTTGTGTAATTATTTGATTTTTTTATTTACTTTCCATCAATATTTTAAAAATACCAAGCTTAAAATTTGAAAATTAAAAAAGAATCAGAAAGTATTTTTTACTTTTAGAATTTGAAAAAAAAAAATATATATATATATATATAGTCTTTCATAAAAATGTAAGAACTATAATACAACAATATATAAGAAAACAAACATATATTTTAGTAAAAATATATATATATATATATATATATAAATATATATATTTTAGTAAATATATATATATTTTAGTAAATATATATATAAGAAAACAAACATATATTTTAGTAAATATATATATAAGAAAACAAACATATATTTTAGTAAAAAAATATATATATATATATTTTAGTAAATATATATATAAGAAAACAAACATATATTTTAGTAAAAAAAAATATATATATATATATAATATATATATATATATATATATTTTTTTTACTAAATATATGTTTGTTTTCTTATATATTGTTGTATTATAGTTCTTACATTTTTATGAAAGACTATATATATATATACCTGACCCATTTAATAACCATTTAGTTTTTAGTGTATATTTTCTCTCATTTTTTTACTAAAATTTTATCTTTTTTTATTTAACCAATTTTTAGTAGAAAAAGCAACCTAATAAATTTAGAGGTGAAATGAGTATTTATAAAATTAATTTTAAAAAATAGAAAATCAAAATGATTATAAAAATAATTAAATAATGTAATTTCTACAACTAAATAGTCATCGCTTAAACAATTATATAAGGGAATTTTCCTTTTTTAATGAACAGGAAATGGAGAGACAGTGAAATGTTCAAATGAAGAAAACTCAGATCTATTCCACGCAGTTCTTGGTGGATTAGGACAATTTGGGATCATCACACGAGCAAGAATTCTTCTCGAACCAGCTCCTCAAAGGGTACAATAACAAATAACAAAAAAATTAAATTTTAATTTTTTTTCTTGTCCTTTCCATGCAAATAAATAAAAATAAAAGACATGCAATATTAATTTAATTAAATTAAAAAAATTAAATATATACAGGTTCGATGGATTAGAGTATTGTACTCAAATTTCGAAGCATTTACAAAGGATCAAGAATGGCTAATTTCTTTACATTCAAAATCAAATAATGAGAAATTCGATTACGTGGAAGGATTTGTAATCGTGGACGAAGGCTTAATCAACAACTGGAGATCCTCCTTCTTCTCACCGGCTAATCCGGTCAAAATAACCTCCTTTAATAATAATCACTCTGATGGAGGAGTTTTATACTGCTTGGAGATCACCAAAAACTACCATGAATCCGCATCTCACACCGTTCATCAGGTATATACTCAAAAGTTTTATATTATTTAACCTTTTCTTTTTCATTAATTAACTTCCTTCAACGACATTATCATCATTATCATCCTTTTGTTTTTGCATTATAATCACTCAATCATTGCCATTTTATGTCTTCTTCTTTACCAAAACACACATATAATAATACAAAACAATAAATAAAGTTCGTTTGGAACTTGAAATAAATATTTAAAATTTTACTAATATAGACTTTTAATTATGATTTGGAACTTGGAATAATTTGAAAGTGAAGTAAATTGAGTGAGAAATTTTTTATTTTTTCTGTTGTTGTGAGATTACTGAATTATGAACATTGGAGGGCATAAAAAGTAATAATTATGAGGGGATTTTGCAAGCTGTATTTGGGTTTTGTCTTGAATTTCAATATTAGGGTTGTTTTTTAGTAGTCTCTTTTAAAATGGATTTTTGTGTGGAAAGAGATATATATATATATATATAAAATCCCAAATGATTCAGAGAGTTTTAAGGGAATGTGGGCCAATATTGTTCTTTGTTGCATTATTCTTTTTCAGTTGTGTGTCTCTCTCTGCTGCATGGCTGGAAACTTGTTTGCTTATATATATATATATAGGCACCCAAAGAAAAAAAAAAAAAAAAATTATTATTCTTTTTCTTTTTCTTTTTCAATACTCAAAAAGTAAAATTCAATAAATATATATTTATATAGTGAAATTTTCTCAAATATGGATTTGGAATCTTCTACTGCCATCTTTGTCTTTCCTTGTGTTTGTTCTTCTTCTTTGCCCTCTCAATCCCTTTTGGGTCAAACTATATGGATTATCGCACAAGCTTAAGTGGTCAATATTTTTTATTTTAATTAATCTTTTTTTTTATTTGTTTTAGTACCTGTTCGATAATGATTTTGTTTTAAGAACACAATAGGATAAGGGTAGTTTGGATATAATTAAAATGGTATGATCATAATTGATAAGAATAAAACATAGAAGATCTTGTGTGAATCAATTATTGTTTTAAAATCCTAAGACAGAGAAAAGAACACGACTTTAAGGAATTTCTTTCTTTTTCTTTAATTCAAAATTAAATAATAAACTACCAAATTATTCTCTTTTTTTTTCTTCTTTCTTAATTTTAAGATTGATTGGAATCAAACTTATCCTCTGACAAAAAGTAGCACCTGCTTTGCAACAAAAGCTGTTCTCCATTTTTATTTTTTATATATATATTTCATTTTAATTATGCTTTTTTATTTCATTTTTTCAAAATTCAAACTTCAAAGTACTATTATTGTATGTATATGTTTTTTTTTCCTTTTTTCAATTGACTTAAAACGACGGCGTTTAATCAGGAAGTAGAGGCGTTGCTGAAGGAATTGAATTACATACCCGAGTCGGTTTTCACAACGGACCTAGGTTACGTGGACTTTCTGGACCGGGTTCACAAGGCCGAATTAAAGCTCCGGTCCAAGGGCCTGTGGGATGTCCCACATCCATGGCTAAACCTCTTCGTCCCAAAGTCAAGAATCGCCGATTTTGACCGAGGAGTTTTCAAGGGCATTTTGGGAAATAATACCAGTGGGCCCATCCTCATCTACCCCATGAACAAACACAAGTAAGTTTTTTTCTTTTTAAATAAAATAAAATAGCAATCACTTTCTGCATGCAACATGCAAAATTATTATTATTATTATTATATTATTTTATATAGGGGAGGGGGGAGCTTTTTATAATATAATCCACCTCTCAAAATGTCCATAACATTGTTTCCAAAATTTGATAGCTTTCAAATAGTCCCTACAAGTTTTTCTTTTTTCTTTTTTCTTTTTCTTTTTTTGGTAAAGGTATAAAAAAGTGGACCATTGAAAAGATATTATGCTTCATGTTTGGATGACTCAATGTATTCACTTGGAAAAGAAATTATATATGAGACCGTCATAATGTGGTTAATAGTTCTTCACAGTCAAATTATGAGAGCTAAGGACCTTAAATTTATATCTGATTGTTATTAGGAAGCATCTAAATTTTATGTGGTTGCCTCATTGTATTTCACCTCATTTGTAGGTTAAAGGGTTTATGAAAACGACATCAACTATATCTTTAGCATTAAAAATATTGATTATGAACTAGATTTTTTTTCAACATTTGGGTGTCAAAAAAAGTAAGTGACCATCATGAATTGATCTCATTACTTTTGACATTTTATTTACCACTAGATCAACTCATGACGGTTACAAATGAGTTATATTATTAAAATCATGAAATGGGTATCACAATAACCAAAAGAAAGGGTGAATTAGTTCTTAAAAATTCCACATAAGACATGAGATGGAGGCCTAGCCAGCTTGGATTGATAAATACTTTGAGGGTTTTTTTTTTTAATGGGAAAATCGGACGGTGGTGATTGTCCAAGGGAGAAGATTCTGGTGTCAGGAGATCTTCTCCGTTGGACCTACCAATGGGGATCATGTCCTTGTCCAGTTGACGTATTCAGTTTTGCAGTGTTCTAATGAACATTTTGGCTGTTACTGCACATCTAACGACCCCTACACTTGGGGGTCCCATTCATTGCCTCAAAAACTATCAACTCCATGACCCATCCCTTCTCTTACACCCTTTGTTTGCCATTTTATGACAGTTGTTTCCCTTTTTCCTGTGTAGGTGGGACCCGAGGACCTCCGCCGTGACCCCGGAGGATGATGTGTTCTACCTAGTGGCATTGCTACGGTCAGCGTTGGACAATGGGGAGCCGACGCAGTCCTTGGAGTATTTGAGTCATCAGAATCATCAAATCTTGGCATTCTGTTCCGAAAACCGGATTGAAGTGAAACAATACTTGCCTCACTATACGACGGAGGAGGAGTGGGCCGAGCATTTTGGAGATAAGTGGCCGGAGTTTCAAGCGAGGAAGATGAAGTTCGATCCTCGCCACATTCTCGCCACCGGGCAGAGGATATTCCCACCCTTCAAGCCGGTAAACACAGCTGTATCCCGGTGATCAACAAGACCTGATTGATAAGAGAAAACAGCAAAAATGGCAGCAGTATGCACATACTGTAATGAATTAGGAAAGGAATATTTTGTTGTATATAATGAAGTAGAAAAGAGCAGAGAATCAAAATGTTCTTGAGAAGAGGGCTCTTTCCTTTTCTTTTTTTTTACAGTGCAACACAGCTCAGAATTGTTTTGCCGAGGACCAGAAAAGGACGGTGGATTTTCAGCAACATGGATGGGGTCCTCCATGA

mRNA sequence

ATGCAACACTGCCACCGCCGCCGTCCGATTACCTTCGCCGCCGTGAAAAGTTTCATTAATAATTCAACAATGATGACCACTAAGCTTCTCTTGGCCTTTGCCTTCTGCCGTTTGATAGCCACCGTGGGACTCGACATGGGTCCGGCGGCGGAGCTTCCCCGTCTCGGTATCGACGGCCTTCTCACCGTCGACCCTTCCGATATCGAAACCGCTTCTATCGATTTCGGCCTCATGACTCGAAATCCGCCTCTCGCCGTCTTGCACCCAGCCTCCGCCGATGACGTCGCCAAAGTGATCCGGACGGCGGTTGCGGCGGCGGATACGGCGGAGTATGGTGGGTTTACGGTGTCGGCGAGAGGGCACGGGCATTCGATAAACGGGCAGGCGCAGACGGGGAATGGGGTGGTGATTGAGATGAGTGGCGGCGGAAGACGGCACCGGCGCGGCGGCGGGAGGGGTATTTCGCCGCCTCCGCCGCCGCATGTGGTGTCGGAGAAAGAGAGATTTGTAGACGTATGGGGCGGAGAGCTGTGGATTGATGTACTGAAATCGACGTTGGGATATGGGTTGGCGCCGAGATCGTGGACGGATTATTTGTATTTGACGGTCGGCGGAACGCTGTCGAACGGCGGAATCAGCGGCCAAGCTTTCAACCATGGCCCTCAGATCAGCAATGTCCATGAACTTGACGTCGTCACAGGAAATGGAGAGACAGTGAAATGTTCAAATGAAGAAAACTCAGATCTATTCCACGCAGTTCTTGGTGGATTAGGACAATTTGGGATCATCACACGAGCAAGAATTCTTCTCGAACCAGCTCCTCAAAGGGTTCGATGGATTAGAGTATTGTACTCAAATTTCGAAGCATTTACAAAGGATCAAGAATGGCTAATTTCTTTACATTCAAAATCAAATAATGAGAAATTCGATTACGTGGAAGGATTTGTAATCGTGGACGAAGGCTTAATCAACAACTGGAGATCCTCCTTCTTCTCACCGGCTAATCCGGTCAAAATAACCTCCTTTAATAATAATCACTCTGATGGAGGAGTTTTATACTGCTTGGAGATCACCAAAAACTACCATGAATCCGCATCTCACACCGTTCATCAGGAAGTAGAGGCGTTGCTGAAGGAATTGAATTACATACCCGAGTCGGTTTTCACAACGGACCTAGGTTACGTGGACTTTCTGGACCGGGTTCACAAGGCCGAATTAAAGCTCCGGTCCAAGGGCCTGTGGGATGTCCCACATCCATGGCTAAACCTCTTCGTCCCAAAGTCAAGAATCGCCGATTTTGACCGAGGAGTTTTCAAGGGCATTTTGGGAAATAATACCAGTGGGCCCATCCTCATCTACCCCATGAACAAACACAAGTGGGACCCGAGGACCTCCGCCGTGACCCCGGAGGATGATGTGTTCTACCTAGTGGCATTGCTACGGTCAGCGTTGGACAATGGGGAGCCGACGCAGTCCTTGGAGTATTTGAGTCATCAGAATCATCAAATCTTGGCATTCTGTTCCGAAAACCGGATTGAAGTGAAACAATACTTGCCTCACTATACGACGGAGGAGGAGTGGGCCGAGCATTTTGGAGATAAGTGGCCGGAGTTTCAAGCGAGGAAGATGAAGTTCGATCCTCGCCACATTCTCGCCACCGGGCAGAGGATATTCCCACCCTTCAAGCCGTGCAACACAGCTCAGAATTGTTTTGCCGAGGACCAGAAAAGGACGGTGGATTTTCAGCAACATGGATGGGGTCCTCCATGA

Coding sequence (CDS)

ATGCAACACTGCCACCGCCGCCGTCCGATTACCTTCGCCGCCGTGAAAAGTTTCATTAATAATTCAACAATGATGACCACTAAGCTTCTCTTGGCCTTTGCCTTCTGCCGTTTGATAGCCACCGTGGGACTCGACATGGGTCCGGCGGCGGAGCTTCCCCGTCTCGGTATCGACGGCCTTCTCACCGTCGACCCTTCCGATATCGAAACCGCTTCTATCGATTTCGGCCTCATGACTCGAAATCCGCCTCTCGCCGTCTTGCACCCAGCCTCCGCCGATGACGTCGCCAAAGTGATCCGGACGGCGGTTGCGGCGGCGGATACGGCGGAGTATGGTGGGTTTACGGTGTCGGCGAGAGGGCACGGGCATTCGATAAACGGGCAGGCGCAGACGGGGAATGGGGTGGTGATTGAGATGAGTGGCGGCGGAAGACGGCACCGGCGCGGCGGCGGGAGGGGTATTTCGCCGCCTCCGCCGCCGCATGTGGTGTCGGAGAAAGAGAGATTTGTAGACGTATGGGGCGGAGAGCTGTGGATTGATGTACTGAAATCGACGTTGGGATATGGGTTGGCGCCGAGATCGTGGACGGATTATTTGTATTTGACGGTCGGCGGAACGCTGTCGAACGGCGGAATCAGCGGCCAAGCTTTCAACCATGGCCCTCAGATCAGCAATGTCCATGAACTTGACGTCGTCACAGGAAATGGAGAGACAGTGAAATGTTCAAATGAAGAAAACTCAGATCTATTCCACGCAGTTCTTGGTGGATTAGGACAATTTGGGATCATCACACGAGCAAGAATTCTTCTCGAACCAGCTCCTCAAAGGGTTCGATGGATTAGAGTATTGTACTCAAATTTCGAAGCATTTACAAAGGATCAAGAATGGCTAATTTCTTTACATTCAAAATCAAATAATGAGAAATTCGATTACGTGGAAGGATTTGTAATCGTGGACGAAGGCTTAATCAACAACTGGAGATCCTCCTTCTTCTCACCGGCTAATCCGGTCAAAATAACCTCCTTTAATAATAATCACTCTGATGGAGGAGTTTTATACTGCTTGGAGATCACCAAAAACTACCATGAATCCGCATCTCACACCGTTCATCAGGAAGTAGAGGCGTTGCTGAAGGAATTGAATTACATACCCGAGTCGGTTTTCACAACGGACCTAGGTTACGTGGACTTTCTGGACCGGGTTCACAAGGCCGAATTAAAGCTCCGGTCCAAGGGCCTGTGGGATGTCCCACATCCATGGCTAAACCTCTTCGTCCCAAAGTCAAGAATCGCCGATTTTGACCGAGGAGTTTTCAAGGGCATTTTGGGAAATAATACCAGTGGGCCCATCCTCATCTACCCCATGAACAAACACAAGTGGGACCCGAGGACCTCCGCCGTGACCCCGGAGGATGATGTGTTCTACCTAGTGGCATTGCTACGGTCAGCGTTGGACAATGGGGAGCCGACGCAGTCCTTGGAGTATTTGAGTCATCAGAATCATCAAATCTTGGCATTCTGTTCCGAAAACCGGATTGAAGTGAAACAATACTTGCCTCACTATACGACGGAGGAGGAGTGGGCCGAGCATTTTGGAGATAAGTGGCCGGAGTTTCAAGCGAGGAAGATGAAGTTCGATCCTCGCCACATTCTCGCCACCGGGCAGAGGATATTCCCACCCTTCAAGCCGTGCAACACAGCTCAGAATTGTTTTGCCGAGGACCAGAAAAGGACGGTGGATTTTCAGCAACATGGATGGGGTCCTCCATGA

Protein sequence

MQHCHRRRPITFAAVKSFINNSTMMTTKLLLAFAFCRLIATVGLDMGPAAELPRLGIDGLLTVDPSDIETASIDFGLMTRNPPLAVLHPASADDVAKVIRTAVAAADTAEYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGGRRHRRGGGRGISPPPPPHVVSEKERFVDVWGGELWIDVLKSTLGYGLAPRSWTDYLYLTVGGTLSNGGISGQAFNHGPQISNVHELDVVTGNGETVKCSNEENSDLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSKSNNEKFDYVEGFVIVDEGLINNWRSSFFSPANPVKITSFNNNHSDGGVLYCLEITKNYHESASHTVHQEVEALLKELNYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGILGNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQILAFCSENRIEVKQYLPHYTTEEEWAEHFGDKWPEFQARKMKFDPRHILATGQRIFPPFKPCNTAQNCFAEDQKRTVDFQQHGWGPP
Homology
BLAST of HG10004825 vs. NCBI nr
Match: KAA0065202.1 (cytokinin dehydrogenase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 1052.4 bits (2720), Expect = 1.5e-303
Identity = 514/569 (90.33%), Postives = 537/569 (94.38%), Query Frame = 0

Query: 24  MMTTKLLLAFAFCRLIATVGLDMGPAAELPRLGIDGLLTVDPSDIETASIDFGLMTRNPP 83
           M+TTKLLLAF+FCRLIATVGL+MG A ELPRLGIDGLLTVDP DIETASIDFGLMTR+PP
Sbjct: 1   MITTKLLLAFSFCRLIATVGLNMGLATELPRLGIDGLLTVDPFDIETASIDFGLMTRDPP 60

Query: 84  LAVLHPASADDVAKVIRTAVAAADTAEYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGG 143
           LAVLHPASADDVAK+IRTA  AA   EYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGG
Sbjct: 61  LAVLHPASADDVAKLIRTAAVAAAEEEYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGG 120

Query: 144 RRHRRGGGRGISPPPPPHVVSEKERFVDVWGGELWIDVLKSTLGYGLAPRSWTDYLYLTV 203
           R+HRR GGRG+SPP P  VVSEK RFVDVWGGELWIDVLK TL YGLAPRSWTDYLYL+V
Sbjct: 121 RQHRRVGGRGVSPPVP--VVSEKGRFVDVWGGELWIDVLKWTLEYGLAPRSWTDYLYLSV 180

Query: 204 GGTLSNGGISGQAFNHGPQISNVHELDVVTGNGETVKCSNEENSDLFHAVLGGLGQFGII 263
           GGTLSNGGISGQAFNHGPQISNVHELDVVTGNGE VKCSNE+N++LFH VLGGLGQFGII
Sbjct: 181 GGTLSNGGISGQAFNHGPQISNVHELDVVTGNGEIVKCSNEDNAELFHGVLGGLGQFGII 240

Query: 264 TRARILLEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSKSNN-EKFDYVEGFVIVDEGL 323
           TRARI+LEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSK+N+ EKFDYVEGFVIVDEGL
Sbjct: 241 TRARIILEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSKTNSKEKFDYVEGFVIVDEGL 300

Query: 324 INNWRSSFFSPANPVKITSFNNNHSDGGVLYCLEITKNYHESASHTVHQEVEALLKELNY 383
           INNWRSSFFSPANPVKI+SF+ N SDG VLYCLEITKNYHES+SHT+ QEVEAL+K+LNY
Sbjct: 301 INNWRSSFFSPANPVKISSFSKNKSDGAVLYCLEITKNYHESSSHTLDQEVEALMKQLNY 360

Query: 384 IPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGIL 443
           IPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGIL
Sbjct: 361 IPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGIL 420

Query: 444 GNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQ 503
           GNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQ
Sbjct: 421 GNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQ 480

Query: 504 ILAFCSENRIEVKQYLPHYTTEEEWAEHFGDKWPEFQARKMKFDPRHILATGQRIFPPFK 563
           IL FC E+ IEVKQYLPHYTTEEEWA+HFGDKWPEFQARKMKFDP HILATGQ+IFP FK
Sbjct: 481 ILEFCYESGIEVKQYLPHYTTEEEWADHFGDKWPEFQARKMKFDPHHILATGQKIFPAFK 540

Query: 564 P----CNTAQNCFAEDQKRTVDFQQHGWG 588
           P    CNTAQNCFAEDQKRTVDFQQHG G
Sbjct: 541 PGNTACNTAQNCFAEDQKRTVDFQQHGDG 567

BLAST of HG10004825 vs. NCBI nr
Match: XP_022997350.1 (cytokinin dehydrogenase 5-like [Cucurbita maxima])

HSP 1 Score: 1020.0 bits (2636), Expect = 8.2e-294
Identity = 505/573 (88.13%), Postives = 529/573 (92.32%), Query Frame = 0

Query: 1   MQHCHR---RRPITFAAV--KSFINNSTMMTTKLLLAFAFCRLIATVGLDMGPAAELPRL 60
           MQHCHR   RRPIT AA   K FIN+STMM TKLLLAFAFCRLIATVGL+MGP AELPRL
Sbjct: 1   MQHCHRHHHRRPITAAAANFKLFINDSTMMITKLLLAFAFCRLIATVGLNMGPTAELPRL 60

Query: 61  GIDGLLTVDPSDIETASIDFGLMTRNPPLAVLHPASADDVAKVIRT-AVAAADTAEYGGF 120
           GIDGLL+VDPSD++TAS+DFGLMTRNPPLAVLHPASADDVA++IR  A AA +  EYGGF
Sbjct: 61  GIDGLLSVDPSDVQTASVDFGLMTRNPPLAVLHPASADDVARLIRAEAAAAEEEGEYGGF 120

Query: 121 TVSARGHGHSINGQAQTGNGVVIEMSGGGRRHRRGGGRGISPPPPPHVVSEKERFVDVWG 180
           TVSARGHGHSINGQ QTGNGVVIEM+GGGRRHRRGG R  SPP P  VVSEKE+FVDVWG
Sbjct: 121 TVSARGHGHSINGQTQTGNGVVIEMNGGGRRHRRGGERS-SPPQP--VVSEKEKFVDVWG 180

Query: 181 GELWIDVLKSTLGYGLAPRSWTDYLYLTVGGTLSNGGISGQAFNHGPQISNVHELDVVTG 240
           GELWIDVLKSTLGYGLAPRSWTDYLYL+VGGTLSNGGISGQAFNHGPQISNVHELDVVTG
Sbjct: 181 GELWIDVLKSTLGYGLAPRSWTDYLYLSVGGTLSNGGISGQAFNHGPQISNVHELDVVTG 240

Query: 241 NGETVKCSNEENSDLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFTKDQ 300
           NGE +KCSN+EN DLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFT+DQ
Sbjct: 241 NGEIMKCSNQENPDLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFTRDQ 300

Query: 301 EWLISLHSKSNNEKFDYVEGFVIVDEGLINNWRSSFFSPANPVKITSFNNNHSDGGVLYC 360
           EWLISLH+K  NEKFDYVEGFVIVDE LINNWRSSFFSPANPVKI+SFNN   DGGVLYC
Sbjct: 301 EWLISLHAKPENEKFDYVEGFVIVDESLINNWRSSFFSPANPVKISSFNNK-PDGGVLYC 360

Query: 361 LEITKNYHESASHTVHQEVEALLKELNYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLW 420
           LEITKNYHESAS TV QEVEALLKEL+YIPESVFTTDL YVDFLDRVHKAELKLRSKGLW
Sbjct: 361 LEITKNYHESASPTVDQEVEALLKELDYIPESVFTTDLAYVDFLDRVHKAELKLRSKGLW 420

Query: 421 DVPHPWLNLFVPKSRIADFDRGVFKGILGNNTSGPILIYPMNKHKWDPRTSAVTPEDDVF 480
           DVPHPWLNLFVPKSRIADFDRG+FKGILGNNTSGPILIYPMNKHKWDPR+SAV PEDDVF
Sbjct: 421 DVPHPWLNLFVPKSRIADFDRGIFKGILGNNTSGPILIYPMNKHKWDPRSSAVVPEDDVF 480

Query: 481 YLVALLRSALDNGEPTQSLEYLSHQNHQILAFCSENRIEVKQYLPHYTTEEEWAEHFGDK 540
           YLVALLRSALDNGEPTQSLEYLS QNHQILAFC++  I+VKQYLPHYTTE EWAEHFG+K
Sbjct: 481 YLVALLRSALDNGEPTQSLEYLSDQNHQILAFCAKEGIKVKQYLPHYTTEREWAEHFGEK 540

Query: 541 WPEFQARKMKFDPRHILATGQRIFPPFKPCNTA 568
           WPEFQ RKMKFDPRHILATGQRIF PF   NTA
Sbjct: 541 WPEFQQRKMKFDPRHILATGQRIFRPFTTVNTA 569

BLAST of HG10004825 vs. NCBI nr
Match: KAG6598469.1 (Cytokinin dehydrogenase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.8e-293
Identity = 504/575 (87.65%), Postives = 528/575 (91.83%), Query Frame = 0

Query: 1   MQHCHR---RRPITFAAV--KSFINNSTMMTTKLLLAFAFCRLIATVGLDMGPAAELPRL 60
           MQHCHR   RRPIT AA   K FIN+STMMTTKLLLAFAFCRLIATVGL+MGP AELPRL
Sbjct: 1   MQHCHRHNHRRPITAAAANFKLFINDSTMMTTKLLLAFAFCRLIATVGLNMGPTAELPRL 60

Query: 61  GIDGLLTVDPSDIETASIDFGLMTRNPPLAVLHPASADDVAKVIRTAVAAA---DTAEYG 120
           GIDGLL+VDPSD++ AS+DFGLMTRNPPLAVLHPASADDVA++IR A AAA   +  EYG
Sbjct: 61  GIDGLLSVDPSDVQIASVDFGLMTRNPPLAVLHPASADDVARLIRAATAAAAAEEEGEYG 120

Query: 121 GFTVSARGHGHSINGQAQTGNGVVIEMSGGGRRHRRGGGRGISPPPPPHVVSEKERFVDV 180
           GFTVSARGHGHSINGQ QTGNGVVIEM+GGGRRH RGGG   SPP P  VV+E+E+FVDV
Sbjct: 121 GFTVSARGHGHSINGQTQTGNGVVIEMNGGGRRH-RGGGERSSPPQP--VVAEREKFVDV 180

Query: 181 WGGELWIDVLKSTLGYGLAPRSWTDYLYLTVGGTLSNGGISGQAFNHGPQISNVHELDVV 240
           WGGELWIDVLKSTLGYGLAPRSWTDYLYL+VGGTLSNGGISGQAFNHGPQISNVHELDVV
Sbjct: 181 WGGELWIDVLKSTLGYGLAPRSWTDYLYLSVGGTLSNGGISGQAFNHGPQISNVHELDVV 240

Query: 241 TGNGETVKCSNEENSDLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFTK 300
           TGNGE +KCSNEEN DLFHAVLGGLGQFGIITRARILLEPAP+RVRWIRVLYSNFEAF K
Sbjct: 241 TGNGEIIKCSNEENPDLFHAVLGGLGQFGIITRARILLEPAPERVRWIRVLYSNFEAFRK 300

Query: 301 DQEWLISLHSKSNNEKFDYVEGFVIVDEGLINNWRSSFFSPANPVKITSFNNNHSDGGVL 360
           DQEWLISLH+K  NEKFDYVEGFVIVDE LINNWRSSFFSPANPVKI+SFNN   DG VL
Sbjct: 301 DQEWLISLHAKPENEKFDYVEGFVIVDEALINNWRSSFFSPANPVKISSFNNK-PDGDVL 360

Query: 361 YCLEITKNYHESASHTVHQEVEALLKELNYIPESVFTTDLGYVDFLDRVHKAELKLRSKG 420
           YCLEITKNYHESAS TV QEVEALLKEL+YIPESVFTTDL YVDFLDRVHKAELKLRSKG
Sbjct: 361 YCLEITKNYHESASPTVDQEVEALLKELDYIPESVFTTDLAYVDFLDRVHKAELKLRSKG 420

Query: 421 LWDVPHPWLNLFVPKSRIADFDRGVFKGILGNNTSGPILIYPMNKHKWDPRTSAVTPEDD 480
           LWDVPHPWLNLFVPKSRIADFDRG+FKGILGNNTSGPILIYPMNKHKWDPR+SAV PEDD
Sbjct: 421 LWDVPHPWLNLFVPKSRIADFDRGIFKGILGNNTSGPILIYPMNKHKWDPRSSAVVPEDD 480

Query: 481 VFYLVALLRSALDNGEPTQSLEYLSHQNHQILAFCSENRIEVKQYLPHYTTEEEWAEHFG 540
           VFYLVALLRSALDNGEPTQSLEYLS QNHQILAFC++  I+VKQYLPHYTTE EWAEHFG
Sbjct: 481 VFYLVALLRSALDNGEPTQSLEYLSDQNHQILAFCAKEGIKVKQYLPHYTTEREWAEHFG 540

Query: 541 DKWPEFQARKMKFDPRHILATGQRIFPPFKPCNTA 568
           DKWPEFQ RKMKFDPRHILATGQRIFPPF   NTA
Sbjct: 541 DKWPEFQQRKMKFDPRHILATGQRIFPPFTTVNTA 571

BLAST of HG10004825 vs. NCBI nr
Match: XP_022961617.1 (cytokinin dehydrogenase 5-like [Cucurbita moschata])

HSP 1 Score: 1016.5 bits (2627), Expect = 9.0e-293
Identity = 503/575 (87.48%), Postives = 528/575 (91.83%), Query Frame = 0

Query: 1   MQHCHR---RRPITFAAV--KSFINNSTMMTTKLLLAFAFCRLIATVGLDMGPAAELPRL 60
           MQHCHR   RRPIT AA   K FIN+ST MTTKLLLAFAFCRLIATVGL+MGP AELPRL
Sbjct: 1   MQHCHRHNHRRPITAAAANFKLFINDSTKMTTKLLLAFAFCRLIATVGLNMGPTAELPRL 60

Query: 61  GIDGLLTVDPSDIETASIDFGLMTRNPPLAVLHPASADDVAKVIRTAVAAA---DTAEYG 120
           GIDGLL+VDPSD++ AS+DFGLMTRNPPLAVLHPASADDVA++IR A AAA   +  EYG
Sbjct: 61  GIDGLLSVDPSDVQIASVDFGLMTRNPPLAVLHPASADDVARLIRAAAAAAAAEEEGEYG 120

Query: 121 GFTVSARGHGHSINGQAQTGNGVVIEMSGGGRRHRRGGGRGISPPPPPHVVSEKERFVDV 180
           GFTVSARGHGHSINGQ QTGNGVVIEM+GGGRRHRRGG R  SPP P  VV+E+E+FVDV
Sbjct: 121 GFTVSARGHGHSINGQTQTGNGVVIEMNGGGRRHRRGGERS-SPPQP--VVAEREKFVDV 180

Query: 181 WGGELWIDVLKSTLGYGLAPRSWTDYLYLTVGGTLSNGGISGQAFNHGPQISNVHELDVV 240
           WGGELWIDVLKSTLGYGLAPRSWTDYLYL+VGGTLSNGGISGQAFNHGPQISNVHELDVV
Sbjct: 181 WGGELWIDVLKSTLGYGLAPRSWTDYLYLSVGGTLSNGGISGQAFNHGPQISNVHELDVV 240

Query: 241 TGNGETVKCSNEENSDLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFTK 300
           TGNGE +KCSNEEN DLFHAVLGGLGQFGIITRARILLEPAP+RVRWIRVLYSNFEAF K
Sbjct: 241 TGNGEIIKCSNEENPDLFHAVLGGLGQFGIITRARILLEPAPERVRWIRVLYSNFEAFRK 300

Query: 301 DQEWLISLHSKSNNEKFDYVEGFVIVDEGLINNWRSSFFSPANPVKITSFNNNHSDGGVL 360
           +QEWLISLH+K  NEKFDYVEGFVIVDE LINNWRSSFFSPANPVKI+SFNN   DG VL
Sbjct: 301 NQEWLISLHAKPENEKFDYVEGFVIVDEALINNWRSSFFSPANPVKISSFNNK-PDGDVL 360

Query: 361 YCLEITKNYHESASHTVHQEVEALLKELNYIPESVFTTDLGYVDFLDRVHKAELKLRSKG 420
           YCLEITKNYHESAS TV QEVEALLKEL+YIPESVFTTDL YVDFLDRVHKAELKLRSKG
Sbjct: 361 YCLEITKNYHESASPTVDQEVEALLKELDYIPESVFTTDLAYVDFLDRVHKAELKLRSKG 420

Query: 421 LWDVPHPWLNLFVPKSRIADFDRGVFKGILGNNTSGPILIYPMNKHKWDPRTSAVTPEDD 480
           LWDVPHPWLNLFVPKSRIADFDRG+FKGILGNNTSGPILIYPMNKHKWDPR+SAV PEDD
Sbjct: 421 LWDVPHPWLNLFVPKSRIADFDRGIFKGILGNNTSGPILIYPMNKHKWDPRSSAVVPEDD 480

Query: 481 VFYLVALLRSALDNGEPTQSLEYLSHQNHQILAFCSENRIEVKQYLPHYTTEEEWAEHFG 540
           VFYLVALLRSALDNGEPTQSLEYLS QNHQILAFC++  I+VKQYLPHYTTE EWAEHFG
Sbjct: 481 VFYLVALLRSALDNGEPTQSLEYLSDQNHQILAFCAKEGIKVKQYLPHYTTEREWAEHFG 540

Query: 541 DKWPEFQARKMKFDPRHILATGQRIFPPFKPCNTA 568
           DKWPEFQ RKMKFDPRHILATGQRIFPPF   NTA
Sbjct: 541 DKWPEFQQRKMKFDPRHILATGQRIFPPFTTVNTA 571

BLAST of HG10004825 vs. NCBI nr
Match: XP_008444755.1 (PREDICTED: cytokinin dehydrogenase 5 [Cucumis melo])

HSP 1 Score: 1013.1 bits (2618), Expect = 1.0e-291
Identity = 494/545 (90.64%), Postives = 517/545 (94.86%), Query Frame = 0

Query: 24  MMTTKLLLAFAFCRLIATVGLDMGPAAELPRLGIDGLLTVDPSDIETASIDFGLMTRNPP 83
           M+TTKLLLAF+FCRLIATVGL+MG A ELPRLGIDGLLTVDP DIETASIDFGLMTR+PP
Sbjct: 1   MITTKLLLAFSFCRLIATVGLNMGLATELPRLGIDGLLTVDPFDIETASIDFGLMTRDPP 60

Query: 84  LAVLHPASADDVAKVIRTAVAAADTAEYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGG 143
           LAVLHPASADDVAK+IRTA  AA   EYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGG
Sbjct: 61  LAVLHPASADDVAKLIRTAAVAAAEEEYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGG 120

Query: 144 RRHRRGGGRGISPPPPPHVVSEKERFVDVWGGELWIDVLKSTLGYGLAPRSWTDYLYLTV 203
           R+HRR GGRG+SPP P  VVSEK RFVDVWGGELWIDVLK TL YGLAPRSWTDYLYL+V
Sbjct: 121 RQHRRVGGRGVSPPVP--VVSEKGRFVDVWGGELWIDVLKWTLEYGLAPRSWTDYLYLSV 180

Query: 204 GGTLSNGGISGQAFNHGPQISNVHELDVVTGNGETVKCSNEENSDLFHAVLGGLGQFGII 263
           GGTLSNGGISGQAFNHGPQISNVHELDVVTGNGE VKCSNE+N++LFH VLGGLGQFGII
Sbjct: 181 GGTLSNGGISGQAFNHGPQISNVHELDVVTGNGEIVKCSNEDNAELFHGVLGGLGQFGII 240

Query: 264 TRARILLEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSKSNN-EKFDYVEGFVIVDEGL 323
           TRARI+LEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSK+N+ EKFDYVEGFVIVDEGL
Sbjct: 241 TRARIILEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSKTNSKEKFDYVEGFVIVDEGL 300

Query: 324 INNWRSSFFSPANPVKITSFNNNHSDGGVLYCLEITKNYHESASHTVHQEVEALLKELNY 383
           INNWRSSFFSPANPVKI+SF+ N SDG VLYCLEITKNYHES+SHT+ QEVEAL+K+LNY
Sbjct: 301 INNWRSSFFSPANPVKISSFSKNKSDGAVLYCLEITKNYHESSSHTLDQEVEALMKQLNY 360

Query: 384 IPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGIL 443
           IPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGIL
Sbjct: 361 IPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGIL 420

Query: 444 GNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQ 503
           GNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQ
Sbjct: 421 GNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQ 480

Query: 504 ILAFCSENRIEVKQYLPHYTTEEEWAEHFGDKWPEFQARKMKFDPRHILATGQRIFPPFK 563
           IL FC E+ IEVKQYLPHYTTEEEWA+HFGDKWPEFQARKMKFDP HILATGQ+IFP FK
Sbjct: 481 ILEFCYESGIEVKQYLPHYTTEEEWADHFGDKWPEFQARKMKFDPHHILATGQKIFPAFK 540

Query: 564 PCNTA 568
           P NTA
Sbjct: 541 PGNTA 543

BLAST of HG10004825 vs. ExPASy Swiss-Prot
Match: Q67YU0 (Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 3.9e-214
Identity = 375/543 (69.06%), Postives = 437/543 (80.48%), Query Frame = 0

Query: 21  NSTMMTTKLLLAFAFCRLIATVGLDMGPAAELPRLG---IDGLLTVDPSDIETASIDFGL 80
           N  M ++ LLL FA C+LI  VGL++GP +EL R+G   +DG  TV PSD+ + S DFG+
Sbjct: 2   NREMTSSFLLLTFAICKLIIAVGLNVGP-SELLRIGAIDVDGHFTVHPSDLASVSSDFGM 61

Query: 81  M-TRNPPLAVLHPASADDVAKVIRTAVAAADTAEYGGFTVSARGHGHSINGQAQTG-NGV 140
           + +   PLAVLHP+SA+DVA+++RTA  +A       F VSARGHGHSINGQA  G NGV
Sbjct: 62  LKSPEEPLAVLHPSSAEDVARLVRTAYGSAT-----AFPVSARGHGHSINGQAAAGRNGV 121

Query: 141 VIEMSGGGRRHRRGGGRGISPPPPPHVVSEKERFVDVWGGELWIDVLKSTLGYGLAPRSW 200
           V+EM+            G++  P P +V   E +VDVWGGELW+DVLK TL +GLAP+SW
Sbjct: 122 VVEMN-----------HGVTGTPKP-LVRPDEMYVDVWGGELWVDVLKKTLEHGLAPKSW 181

Query: 201 TDYLYLTVGGTLSNGGISGQAFNHGPQISNVHELDVVTGNGETVKCSNEENSDLFHAVLG 260
           TDYLYLTVGGTLSN GISGQAF+HGPQISNV ELDVVTG GE ++CS EEN+ LFH VLG
Sbjct: 182 TDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEENTRLFHGVLG 241

Query: 261 GLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSKSNNEKFDYVEGF 320
           GLGQFGIITRARI LEPAPQRVRWIRVLYS+F+ FT+DQE+LIS+H +    KFDYVEGF
Sbjct: 242 GLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQL---KFDYVEGF 301

Query: 321 VIVDEGLINNWRSSFFSPANPVKITSFNNNHSDGGVLYCLEITKNYHESASHTVHQEVEA 380
           VIVDEGL+NNWRSSFFSP NPVKI+S ++N   G VLYCLEITKNYH+S S  V QEVE 
Sbjct: 302 VIVDEGLVNNWRSSFFSPRNPVKISSVSSN---GSVLYCLEITKNYHDSDSEIVDQEVEI 361

Query: 381 LLKELNYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDR 440
           L+K+LN+IP SVFTTDL YVDFLDRVHKAELKLRSK LW+VPHPWLNLFVPKSRI+DFD+
Sbjct: 362 LMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISDFDK 421

Query: 441 GVFKGILGNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEY 500
           GVFKGILGN TSGPILIYPMNK KWD R+SAVTP+++VFYLVALLRSAL +GE TQ LEY
Sbjct: 422 GVFKGILGNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQKLEY 481

Query: 501 LSHQNHQILAFCSENRIEVKQYLPHYTTEEEWAEHFGDKWPEFQARKMKFDPRHILATGQ 559
           L  QN +IL FC + +I VKQYLPH+ T+EEW  HFGDKW  F++ K +FDPRHILATGQ
Sbjct: 482 LKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHILATGQ 520

BLAST of HG10004825 vs. ExPASy Swiss-Prot
Match: Q5ZAY9 (Cytokinin dehydrogenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX5 PE=2 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 4.2e-184
Identity = 337/540 (62.41%), Postives = 393/540 (72.78%), Query Frame = 0

Query: 29  LLLAFAFCRLIATVGLDMGPAAELP-RLG--IDGLLTVDPSDIETASIDFGLMTRNPPLA 88
           + + F    LI+TVGL + PA E   +LG    G L+V+PSD+  AS+DFG +T   PLA
Sbjct: 6   VFMVFLIYCLISTVGLPVAPADEAAMQLGGVGGGRLSVEPSDVMEASLDFGRLTSAEPLA 65

Query: 89  VLHPASADDVAKVIRTAVAAADTAEYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGGRR 148
           V HP  A DVA +++ A  +A      G  VSARGHGHSI+GQAQ   GVV++MS G R 
Sbjct: 66  VFHPRGAGDVAALVKAAYGSA-----SGIRVSARGHGHSISGQAQAAGGVVVDMSHGWR- 125

Query: 149 HRRGGGRGISPPPPPHVVSEKERFVDVWGGELWIDVLKSTLGY-GLAPRSWTDYLYLTVG 208
                        P +  +    ++DVWGGELWIDVL  TL + GLAPRSWTDYLYL+VG
Sbjct: 126 -----AEAAERTLPVYSPALGGHYIDVWGGELWIDVLNWTLAHGGLAPRSWTDYLYLSVG 185

Query: 209 GTLSNGGISGQAFNHGPQISNVHELDVVTGNGETVKCSNEENSDLFHAVLGGLGQFGIIT 268
           GTLSN GISGQAF+HGPQISNV+ELDVVTG GE V CS   N DLF   LGGLGQ GIIT
Sbjct: 186 GTLSNAGISGQAFHHGPQISNVYELDVVTGKGEVVTCSESNNPDLFFGALGGLGQLGIIT 245

Query: 269 RARILLEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSKSNNEKFDYVEGFVIVDEGLIN 328
           RARI LEPAP RVRWIR LYSNF  FT DQE LISL  +    +FDYVEGFV+  EGLIN
Sbjct: 246 RARIALEPAPHRVRWIRALYSNFTEFTADQERLISL--QHGGRRFDYVEGFVVAAEGLIN 305

Query: 329 NWRSSFFSPANPVKITSFNNNHSDGGVLYCLEITKNYHESASHTVHQEVEALLKELNYIP 388
           NWRSSFFSP NPVK++S  ++    GVLYCLE+TKNY +S + TV Q+VEALL ELN+IP
Sbjct: 306 NWRSSFFSPQNPVKLSSLKHH---SGVLYCLEVTKNYDDSTAVTVDQDVEALLGELNFIP 365

Query: 389 ESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGILGN 448
            +VFTTDL YVDFLDRVHKAELKLR KG+W+VPHPWLNLFVP SRIADFDRGVF+G+LG+
Sbjct: 366 GTVFTTDLPYVDFLDRVHKAELKLRGKGMWEVPHPWLNLFVPASRIADFDRGVFRGVLGS 425

Query: 449 NTS-GPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNG-EPTQSLEYLSHQNHQ 508
            T+ GPILIYPMN+HKWDPR+S VTPE+DVFYLVA LRSA+    +P QSLE L  QN +
Sbjct: 426 RTAGGPILIYPMNRHKWDPRSSVVTPEEDVFYLVAFLRSAVPGSTDPAQSLEALERQNRE 485

Query: 509 ILAFCSENRIEVKQYLPHYTTEEEWAEHFGDKWPEFQARKMKFDPRHILATGQRIF--PP 561
           IL FC E  I  KQYLP++  + EW  HFG +W  F   K +FDPR +LATGQ IF  PP
Sbjct: 486 ILEFCDEAGIGAKQYLPNHKAQREWEAHFGARWARFARLKAEFDPRAMLATGQGIFDSPP 529

BLAST of HG10004825 vs. ExPASy Swiss-Prot
Match: O22213 (Cytokinin dehydrogenase 1 OS=Arabidopsis thaliana OX=3702 GN=CKX1 PE=1 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 2.1e-151
Identity = 267/510 (52.35%), Postives = 349/510 (68.43%), Query Frame = 0

Query: 50  AELPRLGIDGLLTVDPSDIETASIDFGLMTRNPPLAVLHPASADDVAKVIRTAVAAADTA 109
           + L  L ++G ++ D  D+   + DFG   + PPLA+LHP S  D++ +++  V    T+
Sbjct: 57  SSLVSLDLEGYISFD--DVHNVAKDFGNRYQLPPLAILHPRSVFDISSMMKHIVHLGSTS 116

Query: 110 EYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGGRRHRRGGGRGISPPPPPHVVSEKERF 169
                TV+ARGHGHS+ GQA    GVVI+M                  P   +   K+ +
Sbjct: 117 ---NLTVAARGHGHSLQGQALAHQGVVIKMES-------------LRSPDIRIYKGKQPY 176

Query: 170 VDVWGGELWIDVLKSTLGYGLAPRSWTDYLYLTVGGTLSNGGISGQAFNHGPQISNVHEL 229
           VDV GGE+WI++L+ TL YGL+P+SWTDYL+LTVGGTLSN GISGQAF HGPQI+NV++L
Sbjct: 177 VDVSGGEIWINILRETLKYGLSPKSWTDYLHLTVGGTLSNAGISGQAFKHGPQINNVYQL 236

Query: 230 DVVTGNGETVKCSNEENSDLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEA 289
           ++VTG GE V CS + NS+LF +VLGGLGQFGIITRARI LEPAP  V+WIRVLYS+F A
Sbjct: 237 EIVTGKGEVVTCSEKRNSELFFSVLGGLGQFGIITRARISLEPAPHMVKWIRVLYSDFSA 296

Query: 290 FTKDQEWLISLHSKSNNEKFDYVEGFVIVDE-GLINNWRSSFFSPANPVKITSFNNNHSD 349
           F++DQE+LI     S  + FDYVEGFVI++   L+NNWRSS FSP +  + + F    SD
Sbjct: 297 FSRDQEYLI-----SKEKTFDYVEGFVIINRTDLLNNWRSS-FSPNDSTQASRFK---SD 356

Query: 350 GGVLYCLEITKNYHESASHTVHQEVEALLKELNYIPESVFTTDLGYVDFLDRVHKAELKL 409
           G  LYCLE+ K ++   + ++ QE   LL ELNYIP ++F++++ Y++FLDRVH AE KL
Sbjct: 357 GKTLYCLEVVKYFNPEEASSMDQETGKLLSELNYIPSTLFSSEVPYIEFLDRVHIAERKL 416

Query: 410 RSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGILGNNTSGPILIYPMNKHKWDPRTSAVT 469
           R+KGLW+VPHPWLNL +PKS I  F   VF  IL +N +GPILIYP+N+ KW   TS +T
Sbjct: 417 RAKGLWEVPHPWLNLLIPKSSIYQFATEVFNNILTSNNNGPILIYPVNQSKWKKHTSLIT 476

Query: 470 PEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQILAFCSENRIEVKQYLPHYTTEEEWA 529
           P +D+FYLVA L SA+ N      LEYL  QN +++ FC+   + VKQYLPHY T++EW 
Sbjct: 477 PNEDIFYLVAFLPSAVPNSSGKNDLEYLLKQNQRVMNFCAAANLNVKQYLPHYETQKEWK 536

Query: 530 EHFGDKWPEFQARKMKFDPRHILATGQRIF 559
            HFG +W  F  RK  +DP  ILA GQRIF
Sbjct: 537 SHFGKRWETFAQRKQAYDPLAILAPGQRIF 539

BLAST of HG10004825 vs. ExPASy Swiss-Prot
Match: Q75K78 (Cytokinin dehydrogenase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX9 PE=2 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 4.4e-149
Identity = 274/538 (50.93%), Postives = 361/538 (67.10%), Query Frame = 0

Query: 22  STMMTTKLLLAFAFCRLIATVGLDMGPAAELPRLGIDGLLTVDPSDIETASIDFGLMTRN 81
           S +   KLLL  A   +           + L  L +DG  +    D+  A+ DFG ++  
Sbjct: 4   SLLQYLKLLLLLALGGVTTMHVPKQDVPSSLEELTLDGHFSF--HDVSAAAQDFGNLSSF 63

Query: 82  PPLAVLHPASADDVAKVIRTAVAAADTAEYGGFTVSARGHGHSINGQAQTGNGVVIEMSG 141
           PP+AVLHP S  D+A  IR         E+   TV+ARGHGHS+ GQ+Q   G++I M  
Sbjct: 64  PPVAVLHPGSVADIATTIRHVFL---MGEHSTLTVAARGHGHSLYGQSQAAEGIIISMES 123

Query: 142 GGRRHRRGGGRGISPPPPPHVVSEKERFVDVWGGELWIDVLKSTLGYGLAPRSWTDYLYL 201
                 R    G+SP            +VD  GGELWI+VL  TL YGLAP+SWTDYL+L
Sbjct: 124 LQSNTMR-VNPGVSP------------YVDASGGELWINVLHETLKYGLAPKSWTDYLHL 183

Query: 202 TVGGTLSNGGISGQAFNHGPQISNVHELDVVTGNGETVKCSNEENSDLFHAVLGGLGQFG 261
           TVGGTLSN G+SGQ F HGPQISNV+EL++VTG G+ + CS E+NSDLFHA LGGLGQFG
Sbjct: 184 TVGGTLSNAGVSGQTFRHGPQISNVNELEIVTGRGDVITCSPEQNSDLFHAALGGLGQFG 243

Query: 262 IITRARILLEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSKSNNEKFDYVEGFVIVDE- 321
           +ITRARI LEPAP+ VRW+RVLY +F +FT+DQE LIS       + FDY+EGFVI++  
Sbjct: 244 VITRARIPLEPAPKMVRWLRVLYLDFTSFTEDQEMLISA-----EKTFDYIEGFVIINRT 303

Query: 322 GLINNWRSSFFSPANPVKITSFNNNHSDGGVLYCLEITKNYHESASHTVHQEVEALLKEL 381
           G++NNWRSS F+P +PV+ + F    SDG VL+CLE+TKN++   +  + QEV  LL +L
Sbjct: 304 GILNNWRSS-FNPQDPVRSSQF---ESDGKVLFCLEMTKNFNPDEADVMEQEVNTLLSQL 363

Query: 382 NYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKG 441
            Y+P S+F TD+ Y++FLDRVH +E+KLR+KG+W+VPHPWLN+ +P+S I  F + VF  
Sbjct: 364 RYMPSSLFHTDVTYIEFLDRVHSSEMKLRAKGMWEVPHPWLNIIIPRSMIHKFAKEVFGK 423

Query: 442 ILGNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLSHQN 501
           IL ++ +GPIL+YP+NK +WD RTS V P+++VFYLVA L SAL       ++++    N
Sbjct: 424 ILKDSNNGPILLYPVNKSRWDNRTSVVIPDEEVFYLVAFLSSALG----PHNIKHTLDLN 483

Query: 502 HQILAFCSENRIEVKQYLPHYTTEEEWAEHFGDKWPEFQARKMKFDPRHILATGQRIF 559
           ++I+ F  +  I VKQYLP+YTTE+EW  HFG +W  FQ RK  +DP  ILA GQRIF
Sbjct: 484 YRIIEFSDKAGIGVKQYLPNYTTEQEWQSHFGARWDTFQQRKKAYDPLAILAPGQRIF 510

BLAST of HG10004825 vs. ExPASy Swiss-Prot
Match: Q9LY71 (Cytokinin dehydrogenase 6 OS=Arabidopsis thaliana OX=3702 GN=CKX6 PE=2 SV=2)

HSP 1 Score: 523.9 bits (1348), Expect = 2.4e-147
Identity = 281/557 (50.45%), Postives = 359/557 (64.45%), Query Frame = 0

Query: 3   HCHRRRPITFAAVKSFINNSTMMTTKLLLAFAFCRLIATVGLDMGPAAELPRLGIDGLLT 62
           H    R  T   V+SF        T LLL+    +L       +     LP +G      
Sbjct: 5   HASLLRKRTMLIVRSF--------TILLLSCIAFKLACCFSSSISSLKALPLVG-----H 64

Query: 63  VDPSDIETASIDFGLMTRNPPLAVLHPASADDVAKVIRTAVAAADTAEYGGFTVSARGHG 122
           ++   +  AS DFG   +  PLAVLHP S  D+A  IR        ++    TV+ARG G
Sbjct: 65  LEFEHVHHASKDFGNRYQLIPLAVLHPKSVSDIASTIRHIWMMGTHSQ---LTVAARGRG 124

Query: 123 HSINGQAQTGNGVVIEMSGGGRRHRRGGGRGISPPPPPHVVSEKERFVDVWGGELWIDVL 182
           HS+ GQAQT +G+VI M       ++     +  P P         +VDV GGELWI++L
Sbjct: 125 HSLQGQAQTRHGIVIHME--SLHPQKLQVYSVDSPAP---------YVDVSGGELWINIL 184

Query: 183 KSTLGYGLAPRSWTDYLYLTVGGTLSNGGISGQAFNHGPQISNVHELDVVTGNGETVKCS 242
             TL YGLAP+SWTDYL+LTVGGTLSN GISGQAF HGPQISNVH+L++VTG GE + C+
Sbjct: 185 HETLKYGLAPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEILNCT 244

Query: 243 NEENSDLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHS 302
             +NSDLF+ VLGGLGQFGIITRARI LEPAP  V+WIRVLY +F AF KDQE LIS   
Sbjct: 245 KRQNSDLFNGVLGGLGQFGIITRARIALEPAPTMVKWIRVLYLDFAAFAKDQEQLIS--- 304

Query: 303 KSNNEKFDYVEGFVIVDE-GLINNWRSSFFSPANPVKITSFNNNHSDGGVLYCLEITKNY 362
            +   KFDY+EGFVI++  GL+N+WR S F+   P++ + F     DG  LYCLE+ K  
Sbjct: 305 -AQGHKFDYIEGFVIINRTGLLNSWRLS-FTAEEPLEASQFK---FDGRTLYCLELAKYL 364

Query: 363 HESASHTVHQEVEALLKELNYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWL 422
            +     ++QEV+  L EL+Y+  ++FTT++ Y  FLDRVH +E+KLRSKG W+VPHPWL
Sbjct: 365 KQDNKDVINQEVKETLSELSYVTSTLFTTEVAYEAFLDRVHVSEVKLRSKGQWEVPHPWL 424

Query: 423 NLFVPKSRIADFDRGVFKGILGNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLR 482
           NL VP+S+I +F RGVF  IL + ++GP+++YP+NK KWD +TSAVTPE++VFYLVA+L 
Sbjct: 425 NLLVPRSKINEFARGVFGNILTDTSNGPVIVYPVNKSKWDNQTSAVTPEEEVFYLVAILT 484

Query: 483 SALDNGEPTQSLEYLSHQNHQILAFCSENRIEVKQYLPHYTTEEEWAEHFGDKWPEFQAR 542
           SA         +E +  +N +IL F  E  I +KQYLPHYTT EEW  HFGDKW EF  R
Sbjct: 485 SASPGSAGKDGVEEILRRNRRILEFSEEAGIGLKQYLPHYTTREEWRSHFGDKWGEFVRR 526

Query: 543 KMKFDPRHILATGQRIF 559
           K ++DP  ILA G RIF
Sbjct: 545 KSRYDPLAILAPGHRIF 526

BLAST of HG10004825 vs. ExPASy TrEMBL
Match: A0A5A7VA54 (Cytokinin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005390 PE=3 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 7.2e-304
Identity = 514/569 (90.33%), Postives = 537/569 (94.38%), Query Frame = 0

Query: 24  MMTTKLLLAFAFCRLIATVGLDMGPAAELPRLGIDGLLTVDPSDIETASIDFGLMTRNPP 83
           M+TTKLLLAF+FCRLIATVGL+MG A ELPRLGIDGLLTVDP DIETASIDFGLMTR+PP
Sbjct: 1   MITTKLLLAFSFCRLIATVGLNMGLATELPRLGIDGLLTVDPFDIETASIDFGLMTRDPP 60

Query: 84  LAVLHPASADDVAKVIRTAVAAADTAEYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGG 143
           LAVLHPASADDVAK+IRTA  AA   EYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGG
Sbjct: 61  LAVLHPASADDVAKLIRTAAVAAAEEEYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGG 120

Query: 144 RRHRRGGGRGISPPPPPHVVSEKERFVDVWGGELWIDVLKSTLGYGLAPRSWTDYLYLTV 203
           R+HRR GGRG+SPP P  VVSEK RFVDVWGGELWIDVLK TL YGLAPRSWTDYLYL+V
Sbjct: 121 RQHRRVGGRGVSPPVP--VVSEKGRFVDVWGGELWIDVLKWTLEYGLAPRSWTDYLYLSV 180

Query: 204 GGTLSNGGISGQAFNHGPQISNVHELDVVTGNGETVKCSNEENSDLFHAVLGGLGQFGII 263
           GGTLSNGGISGQAFNHGPQISNVHELDVVTGNGE VKCSNE+N++LFH VLGGLGQFGII
Sbjct: 181 GGTLSNGGISGQAFNHGPQISNVHELDVVTGNGEIVKCSNEDNAELFHGVLGGLGQFGII 240

Query: 264 TRARILLEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSKSNN-EKFDYVEGFVIVDEGL 323
           TRARI+LEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSK+N+ EKFDYVEGFVIVDEGL
Sbjct: 241 TRARIILEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSKTNSKEKFDYVEGFVIVDEGL 300

Query: 324 INNWRSSFFSPANPVKITSFNNNHSDGGVLYCLEITKNYHESASHTVHQEVEALLKELNY 383
           INNWRSSFFSPANPVKI+SF+ N SDG VLYCLEITKNYHES+SHT+ QEVEAL+K+LNY
Sbjct: 301 INNWRSSFFSPANPVKISSFSKNKSDGAVLYCLEITKNYHESSSHTLDQEVEALMKQLNY 360

Query: 384 IPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGIL 443
           IPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGIL
Sbjct: 361 IPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGIL 420

Query: 444 GNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQ 503
           GNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQ
Sbjct: 421 GNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQ 480

Query: 504 ILAFCSENRIEVKQYLPHYTTEEEWAEHFGDKWPEFQARKMKFDPRHILATGQRIFPPFK 563
           IL FC E+ IEVKQYLPHYTTEEEWA+HFGDKWPEFQARKMKFDP HILATGQ+IFP FK
Sbjct: 481 ILEFCYESGIEVKQYLPHYTTEEEWADHFGDKWPEFQARKMKFDPHHILATGQKIFPAFK 540

Query: 564 P----CNTAQNCFAEDQKRTVDFQQHGWG 588
           P    CNTAQNCFAEDQKRTVDFQQHG G
Sbjct: 541 PGNTACNTAQNCFAEDQKRTVDFQQHGDG 567

BLAST of HG10004825 vs. ExPASy TrEMBL
Match: A0A6J1K9C4 (Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111492293 PE=3 SV=1)

HSP 1 Score: 1020.0 bits (2636), Expect = 4.0e-294
Identity = 505/573 (88.13%), Postives = 529/573 (92.32%), Query Frame = 0

Query: 1   MQHCHR---RRPITFAAV--KSFINNSTMMTTKLLLAFAFCRLIATVGLDMGPAAELPRL 60
           MQHCHR   RRPIT AA   K FIN+STMM TKLLLAFAFCRLIATVGL+MGP AELPRL
Sbjct: 1   MQHCHRHHHRRPITAAAANFKLFINDSTMMITKLLLAFAFCRLIATVGLNMGPTAELPRL 60

Query: 61  GIDGLLTVDPSDIETASIDFGLMTRNPPLAVLHPASADDVAKVIRT-AVAAADTAEYGGF 120
           GIDGLL+VDPSD++TAS+DFGLMTRNPPLAVLHPASADDVA++IR  A AA +  EYGGF
Sbjct: 61  GIDGLLSVDPSDVQTASVDFGLMTRNPPLAVLHPASADDVARLIRAEAAAAEEEGEYGGF 120

Query: 121 TVSARGHGHSINGQAQTGNGVVIEMSGGGRRHRRGGGRGISPPPPPHVVSEKERFVDVWG 180
           TVSARGHGHSINGQ QTGNGVVIEM+GGGRRHRRGG R  SPP P  VVSEKE+FVDVWG
Sbjct: 121 TVSARGHGHSINGQTQTGNGVVIEMNGGGRRHRRGGERS-SPPQP--VVSEKEKFVDVWG 180

Query: 181 GELWIDVLKSTLGYGLAPRSWTDYLYLTVGGTLSNGGISGQAFNHGPQISNVHELDVVTG 240
           GELWIDVLKSTLGYGLAPRSWTDYLYL+VGGTLSNGGISGQAFNHGPQISNVHELDVVTG
Sbjct: 181 GELWIDVLKSTLGYGLAPRSWTDYLYLSVGGTLSNGGISGQAFNHGPQISNVHELDVVTG 240

Query: 241 NGETVKCSNEENSDLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFTKDQ 300
           NGE +KCSN+EN DLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFT+DQ
Sbjct: 241 NGEIMKCSNQENPDLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFTRDQ 300

Query: 301 EWLISLHSKSNNEKFDYVEGFVIVDEGLINNWRSSFFSPANPVKITSFNNNHSDGGVLYC 360
           EWLISLH+K  NEKFDYVEGFVIVDE LINNWRSSFFSPANPVKI+SFNN   DGGVLYC
Sbjct: 301 EWLISLHAKPENEKFDYVEGFVIVDESLINNWRSSFFSPANPVKISSFNNK-PDGGVLYC 360

Query: 361 LEITKNYHESASHTVHQEVEALLKELNYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLW 420
           LEITKNYHESAS TV QEVEALLKEL+YIPESVFTTDL YVDFLDRVHKAELKLRSKGLW
Sbjct: 361 LEITKNYHESASPTVDQEVEALLKELDYIPESVFTTDLAYVDFLDRVHKAELKLRSKGLW 420

Query: 421 DVPHPWLNLFVPKSRIADFDRGVFKGILGNNTSGPILIYPMNKHKWDPRTSAVTPEDDVF 480
           DVPHPWLNLFVPKSRIADFDRG+FKGILGNNTSGPILIYPMNKHKWDPR+SAV PEDDVF
Sbjct: 421 DVPHPWLNLFVPKSRIADFDRGIFKGILGNNTSGPILIYPMNKHKWDPRSSAVVPEDDVF 480

Query: 481 YLVALLRSALDNGEPTQSLEYLSHQNHQILAFCSENRIEVKQYLPHYTTEEEWAEHFGDK 540
           YLVALLRSALDNGEPTQSLEYLS QNHQILAFC++  I+VKQYLPHYTTE EWAEHFG+K
Sbjct: 481 YLVALLRSALDNGEPTQSLEYLSDQNHQILAFCAKEGIKVKQYLPHYTTEREWAEHFGEK 540

Query: 541 WPEFQARKMKFDPRHILATGQRIFPPFKPCNTA 568
           WPEFQ RKMKFDPRHILATGQRIF PF   NTA
Sbjct: 541 WPEFQQRKMKFDPRHILATGQRIFRPFTTVNTA 569

BLAST of HG10004825 vs. ExPASy TrEMBL
Match: A0A6J1HAN3 (Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111462337 PE=3 SV=1)

HSP 1 Score: 1016.5 bits (2627), Expect = 4.4e-293
Identity = 503/575 (87.48%), Postives = 528/575 (91.83%), Query Frame = 0

Query: 1   MQHCHR---RRPITFAAV--KSFINNSTMMTTKLLLAFAFCRLIATVGLDMGPAAELPRL 60
           MQHCHR   RRPIT AA   K FIN+ST MTTKLLLAFAFCRLIATVGL+MGP AELPRL
Sbjct: 1   MQHCHRHNHRRPITAAAANFKLFINDSTKMTTKLLLAFAFCRLIATVGLNMGPTAELPRL 60

Query: 61  GIDGLLTVDPSDIETASIDFGLMTRNPPLAVLHPASADDVAKVIRTAVAAA---DTAEYG 120
           GIDGLL+VDPSD++ AS+DFGLMTRNPPLAVLHPASADDVA++IR A AAA   +  EYG
Sbjct: 61  GIDGLLSVDPSDVQIASVDFGLMTRNPPLAVLHPASADDVARLIRAAAAAAAAEEEGEYG 120

Query: 121 GFTVSARGHGHSINGQAQTGNGVVIEMSGGGRRHRRGGGRGISPPPPPHVVSEKERFVDV 180
           GFTVSARGHGHSINGQ QTGNGVVIEM+GGGRRHRRGG R  SPP P  VV+E+E+FVDV
Sbjct: 121 GFTVSARGHGHSINGQTQTGNGVVIEMNGGGRRHRRGGERS-SPPQP--VVAEREKFVDV 180

Query: 181 WGGELWIDVLKSTLGYGLAPRSWTDYLYLTVGGTLSNGGISGQAFNHGPQISNVHELDVV 240
           WGGELWIDVLKSTLGYGLAPRSWTDYLYL+VGGTLSNGGISGQAFNHGPQISNVHELDVV
Sbjct: 181 WGGELWIDVLKSTLGYGLAPRSWTDYLYLSVGGTLSNGGISGQAFNHGPQISNVHELDVV 240

Query: 241 TGNGETVKCSNEENSDLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFTK 300
           TGNGE +KCSNEEN DLFHAVLGGLGQFGIITRARILLEPAP+RVRWIRVLYSNFEAF K
Sbjct: 241 TGNGEIIKCSNEENPDLFHAVLGGLGQFGIITRARILLEPAPERVRWIRVLYSNFEAFRK 300

Query: 301 DQEWLISLHSKSNNEKFDYVEGFVIVDEGLINNWRSSFFSPANPVKITSFNNNHSDGGVL 360
           +QEWLISLH+K  NEKFDYVEGFVIVDE LINNWRSSFFSPANPVKI+SFNN   DG VL
Sbjct: 301 NQEWLISLHAKPENEKFDYVEGFVIVDEALINNWRSSFFSPANPVKISSFNNK-PDGDVL 360

Query: 361 YCLEITKNYHESASHTVHQEVEALLKELNYIPESVFTTDLGYVDFLDRVHKAELKLRSKG 420
           YCLEITKNYHESAS TV QEVEALLKEL+YIPESVFTTDL YVDFLDRVHKAELKLRSKG
Sbjct: 361 YCLEITKNYHESASPTVDQEVEALLKELDYIPESVFTTDLAYVDFLDRVHKAELKLRSKG 420

Query: 421 LWDVPHPWLNLFVPKSRIADFDRGVFKGILGNNTSGPILIYPMNKHKWDPRTSAVTPEDD 480
           LWDVPHPWLNLFVPKSRIADFDRG+FKGILGNNTSGPILIYPMNKHKWDPR+SAV PEDD
Sbjct: 421 LWDVPHPWLNLFVPKSRIADFDRGIFKGILGNNTSGPILIYPMNKHKWDPRSSAVVPEDD 480

Query: 481 VFYLVALLRSALDNGEPTQSLEYLSHQNHQILAFCSENRIEVKQYLPHYTTEEEWAEHFG 540
           VFYLVALLRSALDNGEPTQSLEYLS QNHQILAFC++  I+VKQYLPHYTTE EWAEHFG
Sbjct: 481 VFYLVALLRSALDNGEPTQSLEYLSDQNHQILAFCAKEGIKVKQYLPHYTTEREWAEHFG 540

Query: 541 DKWPEFQARKMKFDPRHILATGQRIFPPFKPCNTA 568
           DKWPEFQ RKMKFDPRHILATGQRIFPPF   NTA
Sbjct: 541 DKWPEFQQRKMKFDPRHILATGQRIFPPFTTVNTA 571

BLAST of HG10004825 vs. ExPASy TrEMBL
Match: A0A1S3BBY3 (Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103488003 PE=3 SV=1)

HSP 1 Score: 1013.1 bits (2618), Expect = 4.8e-292
Identity = 494/545 (90.64%), Postives = 517/545 (94.86%), Query Frame = 0

Query: 24  MMTTKLLLAFAFCRLIATVGLDMGPAAELPRLGIDGLLTVDPSDIETASIDFGLMTRNPP 83
           M+TTKLLLAF+FCRLIATVGL+MG A ELPRLGIDGLLTVDP DIETASIDFGLMTR+PP
Sbjct: 1   MITTKLLLAFSFCRLIATVGLNMGLATELPRLGIDGLLTVDPFDIETASIDFGLMTRDPP 60

Query: 84  LAVLHPASADDVAKVIRTAVAAADTAEYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGG 143
           LAVLHPASADDVAK+IRTA  AA   EYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGG
Sbjct: 61  LAVLHPASADDVAKLIRTAAVAAAEEEYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGG 120

Query: 144 RRHRRGGGRGISPPPPPHVVSEKERFVDVWGGELWIDVLKSTLGYGLAPRSWTDYLYLTV 203
           R+HRR GGRG+SPP P  VVSEK RFVDVWGGELWIDVLK TL YGLAPRSWTDYLYL+V
Sbjct: 121 RQHRRVGGRGVSPPVP--VVSEKGRFVDVWGGELWIDVLKWTLEYGLAPRSWTDYLYLSV 180

Query: 204 GGTLSNGGISGQAFNHGPQISNVHELDVVTGNGETVKCSNEENSDLFHAVLGGLGQFGII 263
           GGTLSNGGISGQAFNHGPQISNVHELDVVTGNGE VKCSNE+N++LFH VLGGLGQFGII
Sbjct: 181 GGTLSNGGISGQAFNHGPQISNVHELDVVTGNGEIVKCSNEDNAELFHGVLGGLGQFGII 240

Query: 264 TRARILLEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSKSNN-EKFDYVEGFVIVDEGL 323
           TRARI+LEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSK+N+ EKFDYVEGFVIVDEGL
Sbjct: 241 TRARIILEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSKTNSKEKFDYVEGFVIVDEGL 300

Query: 324 INNWRSSFFSPANPVKITSFNNNHSDGGVLYCLEITKNYHESASHTVHQEVEALLKELNY 383
           INNWRSSFFSPANPVKI+SF+ N SDG VLYCLEITKNYHES+SHT+ QEVEAL+K+LNY
Sbjct: 301 INNWRSSFFSPANPVKISSFSKNKSDGAVLYCLEITKNYHESSSHTLDQEVEALMKQLNY 360

Query: 384 IPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGIL 443
           IPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGIL
Sbjct: 361 IPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGIL 420

Query: 444 GNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQ 503
           GNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQ
Sbjct: 421 GNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQ 480

Query: 504 ILAFCSENRIEVKQYLPHYTTEEEWAEHFGDKWPEFQARKMKFDPRHILATGQRIFPPFK 563
           IL FC E+ IEVKQYLPHYTTEEEWA+HFGDKWPEFQARKMKFDP HILATGQ+IFP FK
Sbjct: 481 ILEFCYESGIEVKQYLPHYTTEEEWADHFGDKWPEFQARKMKFDPHHILATGQKIFPAFK 540

Query: 564 PCNTA 568
           P NTA
Sbjct: 541 PGNTA 543

BLAST of HG10004825 vs. ExPASy TrEMBL
Match: A0A6J1GIZ0 (Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111454619 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.8e-270
Identity = 476/579 (82.21%), Postives = 499/579 (86.18%), Query Frame = 0

Query: 1   MQHCH--------RRRPITFAAV----KSFINNSTMMTTKLLLAFAFCRLIATVGLDMGP 60
           MQ CH         RR I  AA       F NNS MM +KLLLAFAFCRL+ATVGL+MGP
Sbjct: 1   MQRCHCDHHHRRRHRRSIASAAAVYFKLLFNNNSAMMVSKLLLAFAFCRLMATVGLNMGP 60

Query: 61  AAELPRLGIDGLLTVDPSDIETASIDFGLMTRNPPLAVLHPASADDVAKVIRTAVAAADT 120
           A EL RLGIDGLLT+DPS +ETASIDFGLMTRNPPLAVLHPASADD+A++IR AVA  + 
Sbjct: 61  AVELSRLGIDGLLTIDPSAVETASIDFGLMTRNPPLAVLHPASADDIARLIRAAVATPE- 120

Query: 121 AEYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGGRRHRRGGGRGISPPPPPHVVSEKER 180
            EYGGFTVSARGHGHSINGQAQTGNGVVIEMS G      GG       PPP +VSEKE 
Sbjct: 121 -EYGGFTVSARGHGHSINGQAQTGNGVVIEMSSG-----VGGVDSGRDFPPPPIVSEKEM 180

Query: 181 FVDVWGGELWIDVLKSTLGYGLAPRSWTDYLYLTVGGTLSNGGISGQAFNHGPQISNVHE 240
           FVDVWGGELWIDVLKSTL YGLAPRSWTDYLYL+VGGTLSNGGISGQAFNHGPQISNV E
Sbjct: 181 FVDVWGGELWIDVLKSTLRYGLAPRSWTDYLYLSVGGTLSNGGISGQAFNHGPQISNVLE 240

Query: 241 LDVVTGNGETVKCSNEENSDLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFE 300
           LDVVTGNGE   CSNEEN DLFH VLGGLGQFGIITRARI+LE APQRVRWIRVLYSNF 
Sbjct: 241 LDVVTGNGELTTCSNEENPDLFHGVLGGLGQFGIITRARIVLEQAPQRVRWIRVLYSNFT 300

Query: 301 AFTKDQEWLISLHSKSNNEKFDYVEGFVIVDEGLINNWRSSFFSPANPVKITSFNNNHSD 360
           AFT+DQE LISLH+K  NEKFDYVEGFVIVDEGLINNWRSSFFSPANPVKI+S     +D
Sbjct: 301 AFTRDQERLISLHAKPKNEKFDYVEGFVIVDEGLINNWRSSFFSPANPVKISSLKK--AD 360

Query: 361 GGVLYCLEITKNYHESASHTVHQEVEALLKELNYIPESVFTTDLGYVDFLDRVHKAELKL 420
           GGVLYCLEITKNYHES S TV QEVEALLKEL+YIPESVFTTDLGYVDFLDRVH AELKL
Sbjct: 361 GGVLYCLEITKNYHESQSLTVDQEVEALLKELDYIPESVFTTDLGYVDFLDRVHTAELKL 420

Query: 421 RSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGILGNNTSGPILIYPMNKHKWDPRTSAVT 480
           RSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGILGNNTSGPILIYPMNKHKWDPR+SAVT
Sbjct: 421 RSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGILGNNTSGPILIYPMNKHKWDPRSSAVT 480

Query: 481 PEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQILAFCSENRIEVKQYLPHYTTEEEWA 540
           P+DDVFYLVALLRSA+DNGE TQSLEYLS+QNHQI+AFCS   IEVKQYLPHYT EEEWA
Sbjct: 481 PDDDVFYLVALLRSAIDNGEETQSLEYLSNQNHQIIAFCSNEGIEVKQYLPHYTMEEEWA 540

Query: 541 EHFGDKWPEFQARKMKFDPRHILATGQRIFPPFKPCNTA 568
           EHFGDKWPEFQ RKMKFDP HILATGQRIF P K  + A
Sbjct: 541 EHFGDKWPEFQRRKMKFDPHHILATGQRIFSPSKSVDMA 570

BLAST of HG10004825 vs. TAIR 10
Match: AT1G75450.1 (cytokinin oxidase 5 )

HSP 1 Score: 745.7 bits (1924), Expect = 2.8e-215
Identity = 375/543 (69.06%), Postives = 437/543 (80.48%), Query Frame = 0

Query: 21  NSTMMTTKLLLAFAFCRLIATVGLDMGPAAELPRLG---IDGLLTVDPSDIETASIDFGL 80
           N  M ++ LLL FA C+LI  VGL++GP +EL R+G   +DG  TV PSD+ + S DFG+
Sbjct: 2   NREMTSSFLLLTFAICKLIIAVGLNVGP-SELLRIGAIDVDGHFTVHPSDLASVSSDFGM 61

Query: 81  M-TRNPPLAVLHPASADDVAKVIRTAVAAADTAEYGGFTVSARGHGHSINGQAQTG-NGV 140
           + +   PLAVLHP+SA+DVA+++RTA  +A       F VSARGHGHSINGQA  G NGV
Sbjct: 62  LKSPEEPLAVLHPSSAEDVARLVRTAYGSAT-----AFPVSARGHGHSINGQAAAGRNGV 121

Query: 141 VIEMSGGGRRHRRGGGRGISPPPPPHVVSEKERFVDVWGGELWIDVLKSTLGYGLAPRSW 200
           V+EM+            G++  P P +V   E +VDVWGGELW+DVLK TL +GLAP+SW
Sbjct: 122 VVEMN-----------HGVTGTPKP-LVRPDEMYVDVWGGELWVDVLKKTLEHGLAPKSW 181

Query: 201 TDYLYLTVGGTLSNGGISGQAFNHGPQISNVHELDVVTGNGETVKCSNEENSDLFHAVLG 260
           TDYLYLTVGGTLSN GISGQAF+HGPQISNV ELDVVTG GE ++CS EEN+ LFH VLG
Sbjct: 182 TDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEENTRLFHGVLG 241

Query: 261 GLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSKSNNEKFDYVEGF 320
           GLGQFGIITRARI LEPAPQRVRWIRVLYS+F+ FT+DQE+LIS+H +    KFDYVEGF
Sbjct: 242 GLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQL---KFDYVEGF 301

Query: 321 VIVDEGLINNWRSSFFSPANPVKITSFNNNHSDGGVLYCLEITKNYHESASHTVHQEVEA 380
           VIVDEGL+NNWRSSFFSP NPVKI+S ++N   G VLYCLEITKNYH+S S  V QEVE 
Sbjct: 302 VIVDEGLVNNWRSSFFSPRNPVKISSVSSN---GSVLYCLEITKNYHDSDSEIVDQEVEI 361

Query: 381 LLKELNYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDR 440
           L+K+LN+IP SVFTTDL YVDFLDRVHKAELKLRSK LW+VPHPWLNLFVPKSRI+DFD+
Sbjct: 362 LMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISDFDK 421

Query: 441 GVFKGILGNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEY 500
           GVFKGILGN TSGPILIYPMNK KWD R+SAVTP+++VFYLVALLRSAL +GE TQ LEY
Sbjct: 422 GVFKGILGNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQKLEY 481

Query: 501 LSHQNHQILAFCSENRIEVKQYLPHYTTEEEWAEHFGDKWPEFQARKMKFDPRHILATGQ 559
           L  QN +IL FC + +I VKQYLPH+ T+EEW  HFGDKW  F++ K +FDPRHILATGQ
Sbjct: 482 LKDQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHILATGQ 520

BLAST of HG10004825 vs. TAIR 10
Match: AT1G75450.2 (cytokinin oxidase 5 )

HSP 1 Score: 739.2 bits (1907), Expect = 2.6e-213
Identity = 374/541 (69.13%), Postives = 436/541 (80.59%), Query Frame = 0

Query: 24  MMTTKLLLAFAFCRLIATVGLDMGPAAELPRLG---IDGLLTVDPSDIETASIDFGLM-T 83
           M ++ LLL FA C+LI  VGL++GP +EL R+G   +DG  TV PSD+ + S DFG++ +
Sbjct: 1   MTSSFLLLTFAICKLIIAVGLNVGP-SELLRIGAIDVDGHFTVHPSDLASVSSDFGMLKS 60

Query: 84  RNPPLAVLHPASADDVAKVIRTAVAAADTAEYGGFTVSARGHGHSINGQAQTG-NGVVIE 143
              PLAVLHP+SA+DVA+++RTA  +A       F VSARGHGHSINGQA  G NGVV+E
Sbjct: 61  PEEPLAVLHPSSAEDVARLVRTAYGSAT-----AFPVSARGHGHSINGQAAAGRNGVVVE 120

Query: 144 MSGGGRRHRRGGGRGISPPPPPHVVSEKERFVDVWGGELWIDVLKSTLGYGLAPRSWTDY 203
           M+            G++  P P +V   E +VDVWGGELW+DVLK TL +GLAP+SWTDY
Sbjct: 121 MN-----------HGVTGTPKP-LVRPDEMYVDVWGGELWVDVLKKTLEHGLAPKSWTDY 180

Query: 204 LYLTVGGTLSNGGISGQAFNHGPQISNVHELDVVT-GNGETVKCSNEENSDLFHAVLGGL 263
           LYLTVGGTLSN GISGQAF+HGPQISNV ELDVVT G GE ++CS EEN+ LFH VLGGL
Sbjct: 181 LYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTVGKGEVMRCSEEENTRLFHGVLGGL 240

Query: 264 GQFGIITRARILLEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHSKSNNEKFDYVEGFVI 323
           GQFGIITRARI LEPAPQRVRWIRVLYS+F+ FT+DQE+LIS+H +    KFDYVEGFVI
Sbjct: 241 GQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQL---KFDYVEGFVI 300

Query: 324 VDEGLINNWRSSFFSPANPVKITSFNNNHSDGGVLYCLEITKNYHESASHTVHQEVEALL 383
           VDEGL+NNWRSSFFSP NPVKI+S ++N   G VLYCLEITKNYH+S S  V QEVE L+
Sbjct: 301 VDEGLVNNWRSSFFSPRNPVKISSVSSN---GSVLYCLEITKNYHDSDSEIVDQEVEILM 360

Query: 384 KELNYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWLNLFVPKSRIADFDRGV 443
           K+LN+IP SVFTTDL YVDFLDRVHKAELKLRSK LW+VPHPWLNLFVPKSRI+DFD+GV
Sbjct: 361 KKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPWLNLFVPKSRISDFDKGV 420

Query: 444 FKGILGNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLRSALDNGEPTQSLEYLS 503
           FKGILGN TSGPILIYPMNK KWD R+SAVTP+++VFYLVALLRSAL +GE TQ LEYL 
Sbjct: 421 FKGILGNKTSGPILIYPMNKDKWDERSSAVTPDEEVFYLVALLRSALTDGEETQKLEYLK 480

Query: 504 HQNHQILAFCSENRIEVKQYLPHYTTEEEWAEHFGDKWPEFQARKMKFDPRHILATGQRI 559
            QN +IL FC + +I VKQYLPH+ T+EEW  HFGDKW  F++ K +FDPRHILATGQRI
Sbjct: 481 DQNRRILEFCEQAKINVKQYLPHHATQEEWVAHFGDKWDRFRSLKAEFDPRHILATGQRI 517

BLAST of HG10004825 vs. TAIR 10
Match: AT2G41510.1 (cytokinin oxidase/dehydrogenase 1 )

HSP 1 Score: 537.3 bits (1383), Expect = 1.5e-152
Identity = 267/510 (52.35%), Postives = 349/510 (68.43%), Query Frame = 0

Query: 50  AELPRLGIDGLLTVDPSDIETASIDFGLMTRNPPLAVLHPASADDVAKVIRTAVAAADTA 109
           + L  L ++G ++ D  D+   + DFG   + PPLA+LHP S  D++ +++  V    T+
Sbjct: 57  SSLVSLDLEGYISFD--DVHNVAKDFGNRYQLPPLAILHPRSVFDISSMMKHIVHLGSTS 116

Query: 110 EYGGFTVSARGHGHSINGQAQTGNGVVIEMSGGGRRHRRGGGRGISPPPPPHVVSEKERF 169
                TV+ARGHGHS+ GQA    GVVI+M                  P   +   K+ +
Sbjct: 117 ---NLTVAARGHGHSLQGQALAHQGVVIKMES-------------LRSPDIRIYKGKQPY 176

Query: 170 VDVWGGELWIDVLKSTLGYGLAPRSWTDYLYLTVGGTLSNGGISGQAFNHGPQISNVHEL 229
           VDV GGE+WI++L+ TL YGL+P+SWTDYL+LTVGGTLSN GISGQAF HGPQI+NV++L
Sbjct: 177 VDVSGGEIWINILRETLKYGLSPKSWTDYLHLTVGGTLSNAGISGQAFKHGPQINNVYQL 236

Query: 230 DVVTGNGETVKCSNEENSDLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEA 289
           ++VTG GE V CS + NS+LF +VLGGLGQFGIITRARI LEPAP  V+WIRVLYS+F A
Sbjct: 237 EIVTGKGEVVTCSEKRNSELFFSVLGGLGQFGIITRARISLEPAPHMVKWIRVLYSDFSA 296

Query: 290 FTKDQEWLISLHSKSNNEKFDYVEGFVIVDE-GLINNWRSSFFSPANPVKITSFNNNHSD 349
           F++DQE+LI     S  + FDYVEGFVI++   L+NNWRSS FSP +  + + F    SD
Sbjct: 297 FSRDQEYLI-----SKEKTFDYVEGFVIINRTDLLNNWRSS-FSPNDSTQASRFK---SD 356

Query: 350 GGVLYCLEITKNYHESASHTVHQEVEALLKELNYIPESVFTTDLGYVDFLDRVHKAELKL 409
           G  LYCLE+ K ++   + ++ QE   LL ELNYIP ++F++++ Y++FLDRVH AE KL
Sbjct: 357 GKTLYCLEVVKYFNPEEASSMDQETGKLLSELNYIPSTLFSSEVPYIEFLDRVHIAERKL 416

Query: 410 RSKGLWDVPHPWLNLFVPKSRIADFDRGVFKGILGNNTSGPILIYPMNKHKWDPRTSAVT 469
           R+KGLW+VPHPWLNL +PKS I  F   VF  IL +N +GPILIYP+N+ KW   TS +T
Sbjct: 417 RAKGLWEVPHPWLNLLIPKSSIYQFATEVFNNILTSNNNGPILIYPVNQSKWKKHTSLIT 476

Query: 470 PEDDVFYLVALLRSALDNGEPTQSLEYLSHQNHQILAFCSENRIEVKQYLPHYTTEEEWA 529
           P +D+FYLVA L SA+ N      LEYL  QN +++ FC+   + VKQYLPHY T++EW 
Sbjct: 477 PNEDIFYLVAFLPSAVPNSSGKNDLEYLLKQNQRVMNFCAAANLNVKQYLPHYETQKEWK 536

Query: 530 EHFGDKWPEFQARKMKFDPRHILATGQRIF 559
            HFG +W  F  RK  +DP  ILA GQRIF
Sbjct: 537 SHFGKRWETFAQRKQAYDPLAILAPGQRIF 539

BLAST of HG10004825 vs. TAIR 10
Match: AT3G63440.1 (cytokinin oxidase/dehydrogenase 6 )

HSP 1 Score: 523.9 bits (1348), Expect = 1.7e-148
Identity = 281/557 (50.45%), Postives = 359/557 (64.45%), Query Frame = 0

Query: 3   HCHRRRPITFAAVKSFINNSTMMTTKLLLAFAFCRLIATVGLDMGPAAELPRLGIDGLLT 62
           H    R  T   V+SF        T LLL+    +L       +     LP +G      
Sbjct: 5   HASLLRKRTMLIVRSF--------TILLLSCIAFKLACCFSSSISSLKALPLVG-----H 64

Query: 63  VDPSDIETASIDFGLMTRNPPLAVLHPASADDVAKVIRTAVAAADTAEYGGFTVSARGHG 122
           ++   +  AS DFG   +  PLAVLHP S  D+A  IR        ++    TV+ARG G
Sbjct: 65  LEFEHVHHASKDFGNRYQLIPLAVLHPKSVSDIASTIRHIWMMGTHSQ---LTVAARGRG 124

Query: 123 HSINGQAQTGNGVVIEMSGGGRRHRRGGGRGISPPPPPHVVSEKERFVDVWGGELWIDVL 182
           HS+ GQAQT +G+VI M       ++     +  P P         +VDV GGELWI++L
Sbjct: 125 HSLQGQAQTRHGIVIHME--SLHPQKLQVYSVDSPAP---------YVDVSGGELWINIL 184

Query: 183 KSTLGYGLAPRSWTDYLYLTVGGTLSNGGISGQAFNHGPQISNVHELDVVTGNGETVKCS 242
             TL YGLAP+SWTDYL+LTVGGTLSN GISGQAF HGPQISNVH+L++VTG GE + C+
Sbjct: 185 HETLKYGLAPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGEILNCT 244

Query: 243 NEENSDLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFTKDQEWLISLHS 302
             +NSDLF+ VLGGLGQFGIITRARI LEPAP  V+WIRVLY +F AF KDQE LIS   
Sbjct: 245 KRQNSDLFNGVLGGLGQFGIITRARIALEPAPTMVKWIRVLYLDFAAFAKDQEQLIS--- 304

Query: 303 KSNNEKFDYVEGFVIVDE-GLINNWRSSFFSPANPVKITSFNNNHSDGGVLYCLEITKNY 362
            +   KFDY+EGFVI++  GL+N+WR S F+   P++ + F     DG  LYCLE+ K  
Sbjct: 305 -AQGHKFDYIEGFVIINRTGLLNSWRLS-FTAEEPLEASQFK---FDGRTLYCLELAKYL 364

Query: 363 HESASHTVHQEVEALLKELNYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPHPWL 422
            +     ++QEV+  L EL+Y+  ++FTT++ Y  FLDRVH +E+KLRSKG W+VPHPWL
Sbjct: 365 KQDNKDVINQEVKETLSELSYVTSTLFTTEVAYEAFLDRVHVSEVKLRSKGQWEVPHPWL 424

Query: 423 NLFVPKSRIADFDRGVFKGILGNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYLVALLR 482
           NL VP+S+I +F RGVF  IL + ++GP+++YP+NK KWD +TSAVTPE++VFYLVA+L 
Sbjct: 425 NLLVPRSKINEFARGVFGNILTDTSNGPVIVYPVNKSKWDNQTSAVTPEEEVFYLVAILT 484

Query: 483 SALDNGEPTQSLEYLSHQNHQILAFCSENRIEVKQYLPHYTTEEEWAEHFGDKWPEFQAR 542
           SA         +E +  +N +IL F  E  I +KQYLPHYTT EEW  HFGDKW EF  R
Sbjct: 485 SASPGSAGKDGVEEILRRNRRILEFSEEAGIGLKQYLPHYTTREEWRSHFGDKWGEFVRR 526

Query: 543 KMKFDPRHILATGQRIF 559
           K ++DP  ILA G RIF
Sbjct: 545 KSRYDPLAILAPGHRIF 526

BLAST of HG10004825 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 480.7 bits (1236), Expect = 1.7e-135
Identity = 251/503 (49.90%), Postives = 333/503 (66.20%), Query Frame = 0

Query: 59  GLLTVDPSDIETASIDFGLMTRNPPLAVLHPASADDVAKVIRTAVAAADTAEYGGFTVSA 118
           G LT   S +E+A+ DFG +T+  P AVL P+S +D+  +I+ +  +  +     F ++A
Sbjct: 46  GKLTSSSSSVESAATDFGHVTKIFPSAVLIPSSVEDITDLIKLSFDSQLS-----FPLAA 105

Query: 119 RGHGHSINGQAQTGNGVVIEMSGGGRRHRRGGGRGISPPPPPHVVSEKERFVDVWGGELW 178
           RGHGHS  GQA   +GVV+ M     R      RGI        VS    +VDV    LW
Sbjct: 106 RGHGHSHRGQASAKDGVVVNM-----RSMVNRDRGIK-------VSRTCLYVDVDAAWLW 165

Query: 179 IDVLKSTLGYGLAPRSWTDYLYLTVGGTLSNGGISGQAFNHGPQISNVHELDVVTGNGET 238
           I+VL  TL  GL P SWTDYLYLTVGGTLSNGGISGQ F +GPQI+NV E+DV+TG GE 
Sbjct: 166 IEVLNKTLELGLTPVSWTDYLYLTVGGTLSNGGISGQTFRYGPQITNVLEMDVITGKGEI 225

Query: 239 VKCSNEENSDLFHAVLGGLGQFGIITRARILLEPAPQRVRWIRVLYSNFEAFTKDQEWLI 298
             CS + NSDLF AVLGGLGQFGIITRARI LE AP+R +W+R LY +F  FT+DQE +I
Sbjct: 226 ATCSKDMNSDLFFAVLGGLGQFGIITRARIKLEVAPKRAKWLRFLYIDFSEFTRDQERVI 285

Query: 299 SLHSKSNNEKFDYVEGFVIVDEGLINNWRSSFFSPANPVKITSFNNNHSDGGVLYCLEIT 358
                S  +  D++EG ++VD G  +NWRS+++ P++ ++I S    H    V+YCLE+ 
Sbjct: 286 -----SKTDGVDFLEGSIMVDHGPPDNWRSTYYPPSDHLRIASMVKRHR---VIYCLEVV 345

Query: 359 KNYHESASHTVHQEVEALLKELNYIPESVFTTDLGYVDFLDRVHKAELKLRSKGLWDVPH 418
           K Y E++ +TV++E+E L   LN++   ++  D+ Y+DFL+RV   EL L+SKG WDVPH
Sbjct: 346 KYYDETSQYTVNEEMEELSDSLNHVRGFMYEKDVTYMDFLNRVRTGELNLKSKGQWDVPH 405

Query: 419 PWLNLFVPKSRIADFDRGVFKGIL--GNNTSGPILIYPMNKHKWDPRTSAVTPEDDVFYL 478
           PWLNLFVPK++I+ FD GVFKGI+   N TSGP+L+YPMN++KW+ R SA  PE+DVFY 
Sbjct: 406 PWLNLFVPKTQISKFDDGVFKGIILRNNITSGPVLVYPMNRNKWNDRMSAAIPEEDVFYA 465

Query: 479 VALLRSA-LDNGEPTQSLEYLSHQNHQILAFCSENRIEVKQYLPHYTTEEEWAEHFGDKW 538
           V  LRSA  DN E          +N +IL FC +  + V QYLP+++++E W  HFG +W
Sbjct: 466 VGFLRSAGFDNWEA------FDQENMEILKFCEDANMGVIQYLPYHSSQEGWVRHFGPRW 517

Query: 539 PEFQARKMKFDPRHILATGQRIF 559
             F  RK K+DP+ IL+ GQ IF
Sbjct: 526 NIFVERKYKYDPKMILSPGQNIF 517

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0065202.11.5e-30390.33cytokinin dehydrogenase 5 [Cucumis melo var. makuwa][more]
XP_022997350.18.2e-29488.13cytokinin dehydrogenase 5-like [Cucurbita maxima][more]
KAG6598469.11.8e-29387.65Cytokinin dehydrogenase 5, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022961617.19.0e-29387.48cytokinin dehydrogenase 5-like [Cucurbita moschata][more]
XP_008444755.11.0e-29190.64PREDICTED: cytokinin dehydrogenase 5 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q67YU03.9e-21469.06Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1[more]
Q5ZAY94.2e-18462.41Cytokinin dehydrogenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX5 PE=2 ... [more]
O222132.1e-15152.35Cytokinin dehydrogenase 1 OS=Arabidopsis thaliana OX=3702 GN=CKX1 PE=1 SV=1[more]
Q75K784.4e-14950.93Cytokinin dehydrogenase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX9 PE=2 ... [more]
Q9LY712.4e-14750.45Cytokinin dehydrogenase 6 OS=Arabidopsis thaliana OX=3702 GN=CKX6 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7VA547.2e-30490.33Cytokinin dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A6J1K9C44.0e-29488.13Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111492293 PE=3 SV=1[more]
A0A6J1HAN34.4e-29387.48Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111462337 PE=3 SV=1[more]
A0A1S3BBY34.8e-29290.64Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103488003 PE=3 SV=1[more]
A0A6J1GIZ01.8e-27082.21Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111454619 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G75450.12.8e-21569.06cytokinin oxidase 5 [more]
AT1G75450.22.6e-21369.13cytokinin oxidase 5 [more]
AT2G41510.11.5e-15252.35cytokinin oxidase/dehydrogenase 1 [more]
AT3G63440.11.7e-14850.45cytokinin oxidase/dehydrogenase 6 [more]
AT5G56970.11.7e-13549.90cytokinin oxidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 139..275
e-value: 7.0E-222
score: 739.2
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 64..558
e-value: 7.0E-222
score: 739.2
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 83..242
e-value: 2.5E-17
score: 62.9
IPR015345Cytokinin dehydrogenase 1, FAD/cytokinin binding domainPFAMPF09265Cytokin-bindcoord: 274..558
e-value: 1.4E-124
score: 415.0
IPR016170Cytokinin dehydrogenase, C-terminal domain superfamilyGENE3D3.40.462.10coord: 276..517
e-value: 7.0E-222
score: 739.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 141..162
NoneNo IPR availablePANTHERPTHR13878:SF143CYTOKININ OXIDASE 5-RELATEDcoord: 27..560
NoneNo IPR availablePANTHERPTHR13878GULONOLACTONE OXIDASEcoord: 27..560
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 79..273
score: 14.518735
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 58..272
IPR016164FAD-linked oxidase-like, C-terminalSUPERFAMILY55103FAD-linked oxidases, C-terminal domaincoord: 274..558

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10004825.1HG10004825.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009690 cytokinin metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0019139 cytokinin dehydrogenase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity