HG10004508 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10004508
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionCysteine proteinases superfamily protein
LocationChr08: 17775594 .. 17780701 (-)
RNA-Seq ExpressionHG10004508
SyntenyHG10004508
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAACGCCTCCGTCAAAGGAATTGAAGTCTTCGATTTCACCGAAGAAGACGAGCTTCCTGAATTGATCTCCGAGAAGCGCCTCAGCAAATTCAAAAACCCTAATCTCGAATCTAACGCTGTTTTGAAGTATGAATTTCTCGAATGTGGTAGGTCTTCCTCAACTTGAAATTACTTCATTTTTCGTTATTTTAGTACCAGATTTGGATACGAGTCCTTAATTTTTATTGGAAACAGAGTATCGGTTCTTTGGAATAGATGATTTAGGGGGGTTTTTCTGGAGTTATGCGCAGTGAAAGTTGATCGATATATTGGTTCTGTTAGTTTTTTGCCCTAGGTAGATCATGGAGTAGTCAGTAGAATGTTAGGTTCTTTGTTGTCGATTTACGGCCAAATACGGATGAATTTGATACCTATTGAATGGTAGCCGAGTGAATTGAACTAAAATTTCTGGCGACATGTTAACTGTCTGTGGTGTTTCACTATTTGGTATCATCGCTAATTGCTCGCTTACTTCCAATGGGGAATTAAAGTGCATTTTCCCCCCCGCTCTGCGGCTAACTTGCACGCTGTCAAAAGAGGTAATTAGCAATAAGTTTGATCCATTTGGAGATTCACGTCTGAAAAAAGGATTTCTGGAATGATCTTTTTTCATCTTTCATCTATTAAAAAAAAAAAAAAAAACTTATGGAACGGCAAATGTCTTTACTCTACTCTGGTATCATTAATTAGTGAAGATTGCTATTATAATTATTTTCCGAACATGTTTGAATATTTCCTGTATCTGAAAACAAATATAAACTATCGTGATGTTTATATGTGCTGTTTTACTATTTTTCAGTTCTCACTCTATGGGTTGTCCTTGTCTAGGTTATCATTTCAGAATTACTTTCTTTTTGTGCCATTTCTTTAATGAATTTCCGTTCAACAATGCTACTGCAGAACAGTAATCCTTTTTTTTTTTTTTAAATTTTTTTTGGCTATCCTCAAAATTAGTTCCTTTGTTGATGGTCATCCATTTTATAAATTAATGAAGATAAACTAACCGTGACCAATGGATTCGTCGTTGAAGGGAAGGAGATTGAAAATCCACACATGGATGTTGATTTGGATGAATGTAATCGTGGTTGTGATAATGGTATTTCACATCATCCTTCGGTCACAACTAAAGAGCAACAAGACATGGAAGAAGATAAATATCAATTGGATGCCAATACAGAATCAGAAGTGAAATGCCATTCGCAAGATATGCTAATGCAAGTAGATAATCATGTGACACAAAGCCTTTGCTCTGAACTTGGAAAAATTGGATCTAGCTCTCAAAGTCCTACCCTGGGGTTAAATTGCACTCTTCCTGAGTTTACTGCTGAAGTACTTCACTTTCTGCTTTCTGAACATTATTTTCTTTTAAGATATATAGGAGTATCAGTCATGTTCGATTAACACTTTTTTACAATTTACAGAGGGAGCGAGTTGATGCAATGTCAGATCCTAATGGAAGCATGAAAGGGAGTTCTCCAATGAGCCCCCCTTCAGAAACTGTAGAGGATGGTGGTATGTTCTGTATATTGTGTTTACTTCAACATTCTTTTGGTTGTTGTTTTCCAGAGAGGGTGTAAAATGCATCGATAAGAAAGATTACGAAAGGGGACCGATGTTAAGAAAAAAAAGAAAGAAAATAAAAGAAAAAAAAACTCTTCAATCAACTGATTTGAACAGTAAGGGCACATTTCTAATGGCTTTGTTAGTAAACACCAAGGTTGATACCTTGCCACTCTCCTTCCTTCTATTAAAGGAAGTTGTAATTTTTTTTTTCTCTGTTCAAATCTTTCAATGAATGGCCATTGCCCCATTCAACCAGAACAAAGAATTTGAAAAAACCTATATTTACCCCTTCAGTTTGAAAAATTGGACAACAGATCTTCCATCGACTTGGTATACAGCCTGCAGCAAACAGAAGTTTCCAAGTATTTATATCACATATAACTTGCAAGAGTTGCCCTGAATAAAACCTGTCCTCAAAAGAACTCTCTCATAACTCACTCAAATTCATTTTCTGCCTCTGATTTTTTTGGACAATTTTCAGTTTTGTTGAACGGGAAGTCTTCAGATAATTGCTCATCTGACACTGAAAAGGTAAGTTTCTTCCTTCTGATGATTTAAACAGACAGAAAAAAATTCCACACACTTATGTTGTTAATTTTTCCTGAAACTTATTTCCAATTTTTCATTTTTGTTTACTGGGAATCCAGGACTTATGAAGTCTAACTACTTCTATGAGAAGTGTAGGTTGTCCGCATGTTTGTGTTATTTGTATATATTACAAATTCTTCATTATCAGTCCATGTGAAGGGTTTTTTTTTGGTTTATTTATTTATTATTATTTTTTGGGGGATTTTTAAAAATAGAAAAATAAAGGAAACTATTTACACAAAATAGCAAAATTCTTAGATAGTTGTAATAGACGTTGATTGAAGTCTATCAGGGTCTATCAGTGATAGAAATGATAGAAGTCTATCATTGATAGATGCTGATAGAAGTCTATCAATATCTATCTATCAGTGTTTTTTTTTACTATTTTCTGTAAATAGTTTGACATTTTTTCTATCTGTGAAAATTTTCCTTATTTTTTGTTTATCTTGAAGGAGACTGTAGTTTCCTCTTATTCTCAAAGAGTGTTTGAAGGGAAAATCGCTGGTTAGGTTAGGAAGAAAGAATTGAAATAGTGAAACAATATGAATCTTCTTTATTTCACATTTTTTTTTGTGGGTAGTAGAATTGTTTACAACTTATGATGTTTTTTTTTTTTGTTAGTTTGTTAGTTTGTTTGTTTGTGTTTTTTTATTTTTTATTTTTAAAGATTATTATTTTATCAATTCCATAGGCAGATATTGTAGTCATCAAATTTCATTGTCCAGAATATATGCATTTGGGAACTGAAGCTTTCCTCCGTTCAATTTATAGGATGATCTTAACGAGGAGGTTGTCCTTTATCCTGATTATATTGTATGTGGAGATTTTTACTGTGCCAGCCCCTCGTTAACCTTTTCACATAATGGAATAAAAATTAATGGTTTTGCTGATTATGGAAGCAATGAGTTCCTTAACCTTGAATGGAGAGTTGATGATCTCATTCATATCGAGTGCCAGTGCTTTCAAAGGGTGAGTGGTTATCTTTAGAAACTAAATTTCATGATATTTGAGTTCTCTTTGTCGTGATTTTCCAGTTTTCTTTTGGCATACAGGTTGAATATGTGATGATCAAGCTCCATGTTATATCAAAGGATGCTAGTGAATGTGATAATGCGTGTGACACTTCTGGTCAGTGAACTTCTGCAAGGCTACATTGAGCTTTTGGAATTAGATTTCAGTTACATAAAATTGATAGTATTTCAATGTAAATCTTTGATAATTATGGGCTAACATTTACATTCTTCTAGACATCCTCATTGGTTTTTTCCTGTGTTTTCATCAGTGTTCTCAATAATGTGATACAAATCTTTCTTGTCGTTAGTTTTACTAATATGTACTGCCTATGAACTTGATTCAGGCATTAAGGAAGTGAAGATTGTTCTTGTTGATTCTTACTGGTCTGAGAAACAACAAAAAATCAGATCTTTGGATTCCAGATACATGGCTATTTGGAATATTTCTCTGGAGTAAGTTATCTGTTTTTTGTGTCATTATATTGTTGATCATATGTAGTTTTTTAAAAATAGAAGTTATAAACTTGTCATCATGTCAAGACATCAGTTTATGTGGCATATTCTAAAGAAGTATCATGGTTGAGACGGCTATCTGTAAAATATGTCTCTCAGATAACATGCATGCACTGGTTGCATTCGCCAATAAACTTTGTTGTGATTCCCTTTGGCGTATATTGGTCTCCCTTGTTCATTGATTTATTGTTTACCTCTATTATGTCTTGTAGTGTGGGAATAGGAACCGACGACGACGATTTAGGTGGGCAGAGACATTATTTTCCCAAGTGAGTGGAATTTTCTACTTTTTTCTTTTTAAAGCTAGTCTTCATGTTTTAAAATATTTGGTTAGTTTATTTGAAATTTTATTCATATCTACTTCTTAAACTACATTTGTTAATTTTGAGTAAAAGATTCATGTTTACTATGCTATGAGATTTGAAATGTCATGCCTGTGGATCAATCCATTTTCACCTTCTATTTGTAATTGTAAAGTATGCTCTCTTTATTAGATGATCAATACATGAACTTCAGTATATTATCTGGCGGTGGCCACCTTTTTTTCCCCTTCCAATTTATATGAGTCTGTTTGCAGCTTTGATGAGCCTTTTGAAGAAGTTGTCTATCCCAAAGGAGATCCAGATGCCGTTTCCATTAGTAAGAGGGATGTTGACTTGCTGCAACCAGAGACATTTGTCAATGACACAATCATTGACTTTTACATACAGTGAGTCAAACTATAAAGTGATTGTTTGTAATCATGATCGAATACTGTTACTATCAGAGATGTCCCCTCTGTATATTCTCCTAGTGATGTTGGGACTTTCAGCTTCTCATTTAAAGAAGTTCTGGTCCACAATTCTTTTATCTTCAAATTTCAACATTACCTTGCATGGATGACTGGTTTTCATATTAACAAACTTTATTGCGAATATTGGACCACACCCTTTCTAAATGGAATTGATTGGCAGGTATTTGAAGAGCCAGATAGATCTCAAGGAGAAGCATAGATTTCACTTCTTCAATAGTTTTTTCTTTAGGAAGCTGGCAGACCTAGATAAAGATCCATCTAGTGCTTCAGATGGCAGAGCTGCCTTTCTACGGGTTCGGAAATGGACACGAAAAGTGGATTTATTTGACAAGGACTATATCTTCATTCCTATAAACTTCAAGTAAGATTTTTCTCTTCAGGAAAGATTTTTGCCTTGTGTCATAATCTAGACATCACTGGAAGTGGGTATCTTTTTCAACTTTATATGTTCATTATAACAGCCTTCATTGGAGCTTAATGGTCATATGCCATCCTAGCGAAGTGGCTAGATGTAGCGGTAAGTTCTTCATTCATTGGGCCGGTATGCGTCTCCCATCAGTCATAACTTCTGTCTTGTTACAGATGAAAACCTGA

mRNA sequence

ATGAAGAACGCCTCCGTCAAAGGAATTGAAGTCTTCGATTTCACCGAAGAAGACGAGCTTCCTGAATTGATCTCCGAGAAGCGCCTCAGCAAATTCAAAAACCCTAATCTCGAATCTAACGCTGTTTTGAAGTATGAATTTCTCGAATGTGATAAACTAACCGTGACCAATGGATTCGTCGTTGAAGGGAAGGAGATTGAAAATCCACACATGGATGTTGATTTGGATGAATGTAATCGTGGTTGTGATAATGGTATTTCACATCATCCTTCGGTCACAACTAAAGAGCAACAAGACATGGAAGAAGATAAATATCAATTGGATGCCAATACAGAATCAGAAGTGAAATGCCATTCGCAAGATATGCTAATGCAAGTAGATAATCATGTGACACAAAGCCTTTGCTCTGAACTTGGAAAAATTGGATCTAGCTCTCAAAGTCCTACCCTGGGGTTAAATTGCACTCTTCCTGAGTTTACTGCTGAAAGGGAGCGAGTTGATGCAATGTCAGATCCTAATGGAAGCATGAAAGGGAGTTCTCCAATGAGCCCCCCTTCAGAAACTGTAGAGGATGGTGTTTTGTTGAACGGGAAGTCTTCAGATAATTGCTCATCTGACACTGAAAAGGATGATCTTAACGAGGAGGTTGTCCTTTATCCTGATTATATTGTATGTGGAGATTTTTACTGTGCCAGCCCCTCGTTAACCTTTTCACATAATGGAATAAAAATTAATGGTTTTGCTGATTATGGAAGCAATGAGTTCCTTAACCTTGAATGGAGAGTTGATGATCTCATTCATATCGAGTGCCAGTGCTTTCAAAGGGTTGAATATGTGATGATCAAGCTCCATGTTATATCAAAGGATGCTAGTGAATGTGATAATGCGTGTGACACTTCTGGCATTAAGGAAGTGAAGATTGTTCTTGTTGATTCTTACTGGTCTGAGAAACAACAAAAAATCAGATCTTTGGATTCCAGATACATGGCTATTTGGAATATTTCTCTGGATGTGGGAATAGGAACCGACGACGACGATTTAGGTGGGCAGAGACATTATTTTCCCAACTTTGATGAGCCTTTTGAAGAAGTTGTCTATCCCAAAGGAGATCCAGATGCCGTTTCCATTAGTAAGAGGGATGTTGACTTGCTGCAACCAGAGACATTTGTCAATGACACAATCATTGACTTTTACATACAGTATTTGAAGAGCCAGATAGATCTCAAGGAGAAGCATAGATTTCACTTCTTCAATAGTTTTTTCTTTAGGAAGCTGGCAGACCTAGATAAAGATCCATCTAGTGCTTCAGATGGCAGAGCTGCCTTTCTACGGGTTCGGAAATGGACACGAAAAGTGGATTTATTTGACAAGGACTATATCTTCATTCCTATAAACTTCAACCTTCATTGGAGCTTAATGGTCATATGCCATCCTAGCGAAGTGGCTAGATGTAGCGGTAAGTTCTTCATTCATTGGGCCGGTATGCGTCTCCCATCAGTCATAACTTCTGTCTTGTTACAGATGAAAACCTGA

Coding sequence (CDS)

ATGAAGAACGCCTCCGTCAAAGGAATTGAAGTCTTCGATTTCACCGAAGAAGACGAGCTTCCTGAATTGATCTCCGAGAAGCGCCTCAGCAAATTCAAAAACCCTAATCTCGAATCTAACGCTGTTTTGAAGTATGAATTTCTCGAATGTGATAAACTAACCGTGACCAATGGATTCGTCGTTGAAGGGAAGGAGATTGAAAATCCACACATGGATGTTGATTTGGATGAATGTAATCGTGGTTGTGATAATGGTATTTCACATCATCCTTCGGTCACAACTAAAGAGCAACAAGACATGGAAGAAGATAAATATCAATTGGATGCCAATACAGAATCAGAAGTGAAATGCCATTCGCAAGATATGCTAATGCAAGTAGATAATCATGTGACACAAAGCCTTTGCTCTGAACTTGGAAAAATTGGATCTAGCTCTCAAAGTCCTACCCTGGGGTTAAATTGCACTCTTCCTGAGTTTACTGCTGAAAGGGAGCGAGTTGATGCAATGTCAGATCCTAATGGAAGCATGAAAGGGAGTTCTCCAATGAGCCCCCCTTCAGAAACTGTAGAGGATGGTGTTTTGTTGAACGGGAAGTCTTCAGATAATTGCTCATCTGACACTGAAAAGGATGATCTTAACGAGGAGGTTGTCCTTTATCCTGATTATATTGTATGTGGAGATTTTTACTGTGCCAGCCCCTCGTTAACCTTTTCACATAATGGAATAAAAATTAATGGTTTTGCTGATTATGGAAGCAATGAGTTCCTTAACCTTGAATGGAGAGTTGATGATCTCATTCATATCGAGTGCCAGTGCTTTCAAAGGGTTGAATATGTGATGATCAAGCTCCATGTTATATCAAAGGATGCTAGTGAATGTGATAATGCGTGTGACACTTCTGGCATTAAGGAAGTGAAGATTGTTCTTGTTGATTCTTACTGGTCTGAGAAACAACAAAAAATCAGATCTTTGGATTCCAGATACATGGCTATTTGGAATATTTCTCTGGATGTGGGAATAGGAACCGACGACGACGATTTAGGTGGGCAGAGACATTATTTTCCCAACTTTGATGAGCCTTTTGAAGAAGTTGTCTATCCCAAAGGAGATCCAGATGCCGTTTCCATTAGTAAGAGGGATGTTGACTTGCTGCAACCAGAGACATTTGTCAATGACACAATCATTGACTTTTACATACAGTATTTGAAGAGCCAGATAGATCTCAAGGAGAAGCATAGATTTCACTTCTTCAATAGTTTTTTCTTTAGGAAGCTGGCAGACCTAGATAAAGATCCATCTAGTGCTTCAGATGGCAGAGCTGCCTTTCTACGGGTTCGGAAATGGACACGAAAAGTGGATTTATTTGACAAGGACTATATCTTCATTCCTATAAACTTCAACCTTCATTGGAGCTTAATGGTCATATGCCATCCTAGCGAAGTGGCTAGATGTAGCGGTAAGTTCTTCATTCATTGGGCCGGTATGCGTCTCCCATCAGTCATAACTTCTGTCTTGTTACAGATGAAAACCTGA

Protein sequence

MKNASVKGIEVFDFTEEDELPELISEKRLSKFKNPNLESNAVLKYEFLECDKLTVTNGFVVEGKEIENPHMDVDLDECNRGCDNGISHHPSVTTKEQQDMEEDKYQLDANTESEVKCHSQDMLMQVDNHVTQSLCSELGKIGSSSQSPTLGLNCTLPEFTAERERVDAMSDPNGSMKGSSPMSPPSETVEDGVLLNGKSSDNCSSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHNGIKINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTSGIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNISLDVGIGTDDDDLGGQRHYFPNFDEPFEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHPSEVARCSGKFFIHWAGMRLPSVITSVLLQMKT
Homology
BLAST of HG10004508 vs. NCBI nr
Match: XP_038886796.1 (probable ubiquitin-like-specific protease 2B [Benincasa hispida])

HSP 1 Score: 908.3 bits (2346), Expect = 3.0e-260
Identity = 449/501 (89.62%), Postives = 465/501 (92.81%), Query Frame = 0

Query: 1   MKNASVKGIEVFDFTEEDELPELISEKRLSKFKNPNLESNAVLKYEFLECDKLTVTNGFV 60
           MKNASV+ +EVFDFTEEDELPELISEKRLSKFKNPN+ESNAVLKYEFLEC          
Sbjct: 1   MKNASVRSLEVFDFTEEDELPELISEKRLSKFKNPNVESNAVLKYEFLEC---------- 60

Query: 61  VEGKEIENPHMDVDLDECNRGCDNGISHHPSVTTKEQQDMEEDKYQLDANTESEVKCHSQ 120
             GKEIENPHMDVDL+ECN GC NGIS + S TTKEQQ MEE+KYQLD NTE+EVKCHSQ
Sbjct: 61  --GKEIENPHMDVDLEECNHGCANGISLNLSGTTKEQQIMEEEKYQLDPNTETEVKCHSQ 120

Query: 121 DMLMQVDNHVTQSLCSELGKIGSSSQSPTLGLNCTLPEFTAERERVDAMSDPNGSMKGSS 180
           DMLMQVDNHVTQSLCSELGKIGSSSQSPTLGLNCTLPEFTAERE+VDA+S+PNGSMKGSS
Sbjct: 121 DMLMQVDNHVTQSLCSELGKIGSSSQSPTLGLNCTLPEFTAEREQVDALSNPNGSMKGSS 180

Query: 181 PMSPPSETVEDGVLLNGKSSDNCSSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240
           PMSPPSETVEDGVLLNGKSSDNCSSD EKDDLNEEVVLYPDYIVCGDFY ASPSLTFSHN
Sbjct: 181 PMSPPSETVEDGVLLNGKSSDNCSSDNEKDDLNEEVVLYPDYIVCGDFYYASPSLTFSHN 240

Query: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTS 300
           GIKINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDA ECDN CDTS
Sbjct: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDAGECDNVCDTS 300

Query: 301 GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNISLDVGIGTDDDDLGGQRHYFPNFDEP 360
           GIKEVKI LVDSYWSEKQQKIRSLDSRYMAIWNISLDVGIGTDDDDL GQRHYFPNFDEP
Sbjct: 301 GIKEVKIALVDSYWSEKQQKIRSLDSRYMAIWNISLDVGIGTDDDDLDGQRHYFPNFDEP 360

Query: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSF 420
           FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQID KEKHRFHFFNSF
Sbjct: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPKEKHRFHFFNSF 420

Query: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHPSE 480
           FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHP E
Sbjct: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHPGE 480

Query: 481 VARCSGKFFIHWAGMRLPSVI 502
           VARCS +  +    M++P ++
Sbjct: 481 VARCSDENLM--KSMKVPCIL 487

BLAST of HG10004508 vs. NCBI nr
Match: KAA0065091.1 (putative ubiquitin-like-specific protease 2B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 907.5 bits (2344), Expect = 5.1e-260
Identity = 445/498 (89.36%), Postives = 459/498 (92.17%), Query Frame = 0

Query: 1   MKNASVKGIEVFDFTEEDELPELISEKRLSKFKNPNLESNAVLKYEFLECDKLTVTNGFV 60
           MKNAS+KG+EVFDFTEEDELPELISEK L+KFKNPNLESNAV KYEFLEC          
Sbjct: 1   MKNASIKGLEVFDFTEEDELPELISEKHLTKFKNPNLESNAVFKYEFLEC---------- 60

Query: 61  VEGKEIENPHMDVDLDECNRGCDNGISHHPSVTTKEQQDMEEDKYQLDANTESEVKCHSQ 120
             GKEIEN HMDVDLDE   GCDNGIS +PS TTKEQQ MEE+KY+ DANTES+V CHSQ
Sbjct: 61  --GKEIENTHMDVDLDERKLGCDNGISCNPSSTTKEQQVMEEEKYRSDANTESKVNCHSQ 120

Query: 121 DMLMQVDNHVTQSLCSELGKIGSSSQSPTLGLNCTLPEFTAERERVDAMSDPNGSMKGSS 180
           DMLMQ+DNHVTQS CSELGKI SSSQSP LGLNCTLPEF AE++ VD +SDPNGSMKGSS
Sbjct: 121 DMLMQIDNHVTQSQCSELGKIVSSSQSPVLGLNCTLPEFPAEQQHVDGLSDPNGSMKGSS 180

Query: 181 PMSPPSETVEDGVLLNGKSSDNCSSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240
           PMSPPSETVEDGVLLNGKSSDNCSSD EKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN
Sbjct: 181 PMSPPSETVEDGVLLNGKSSDNCSSDNEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240

Query: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTS 300
           GIKINGFADYGSNEFLNLEWRVDDLIHIE QCFQRVEYVMIKLHVISKDA ECDNACDTS
Sbjct: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIESQCFQRVEYVMIKLHVISKDAGECDNACDTS 300

Query: 301 GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNISLDVGIGTDDDDLGGQRHYFPNFDEP 360
           GIKEVKIVLVDSYW EKQQKI+SLDSRYMAIWNISLDVG GTDDDDLGGQRHYFPNFDEP
Sbjct: 301 GIKEVKIVLVDSYWPEKQQKIKSLDSRYMAIWNISLDVGTGTDDDDLGGQRHYFPNFDEP 360

Query: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSF 420
           FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQID KEKHRFHFFNSF
Sbjct: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPKEKHRFHFFNSF 420

Query: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHPSE 480
           FFRKLADLDKDPSSASDGRAAFLRVRKWTRKV+LFDKDYIFIPINFNLHWSLMVICHP E
Sbjct: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVNLFDKDYIFIPINFNLHWSLMVICHPGE 480

Query: 481 VARCSGKFFIHWAGMRLP 499
           VARCSGKFFIHWA +  P
Sbjct: 481 VARCSGKFFIHWANIWSP 486

BLAST of HG10004508 vs. NCBI nr
Match: XP_023546146.1 (probable ubiquitin-like-specific protease 2B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 890.2 bits (2299), Expect = 8.5e-255
Identity = 439/501 (87.62%), Postives = 461/501 (92.02%), Query Frame = 0

Query: 1   MKNASVKGIEVFDFTEEDELPELISEKRLSKFKNPNLESNAVLKYEFLECDKLTVTNGFV 60
           MKNASVKG+EVFDFTEEDELPELISEKRLSKFKNPNL++NAVLKYEFLECDKLTV NGFV
Sbjct: 1   MKNASVKGLEVFDFTEEDELPELISEKRLSKFKNPNLDTNAVLKYEFLECDKLTVANGFV 60

Query: 61  VEGKEIENPHMDVDLDECNRGCDNGISHHPSVTTKEQQDMEEDKYQLDANTESEVKCHSQ 120
           VEGKEI+NPHMDVDL +CNRGCDNGISH PS TTKEQ  MEE KYQLDANTESE+K HSQ
Sbjct: 61  VEGKEIKNPHMDVDLGKCNRGCDNGISHIPSGTTKEQFIMEEGKYQLDANTESELKSHSQ 120

Query: 121 DMLMQVDNHVTQSLCSELGKIGSSSQSPTLGLNCTLPEFTAERERVDAMSDPNGSMKGSS 180
           DM +QVD HVT SL SEL KIGSSSQSP LGLNCTLPEF AERE +DA+SDPNGSM G+S
Sbjct: 121 DMFVQVDGHVTGSLGSELDKIGSSSQSPILGLNCTLPEFNAEREPIDALSDPNGSMNGNS 180

Query: 181 PMSPPSETVEDGVLLNGKSSDNCSSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240
            MSPPSETVEDGVLLNGK  DNCSSD E DDLN+EVVLYPDYIV GDFYCASPSLTFS N
Sbjct: 181 SMSPPSETVEDGVLLNGKYLDNCSSDNEMDDLNKEVVLYPDYIVYGDFYCASPSLTFSPN 240

Query: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTS 300
            IKINGFADYGSNEFLNLEWRVDDLI IECQCFQRVEYVMIKLHVISKDA +CDN+CDTS
Sbjct: 241 AIKINGFADYGSNEFLNLEWRVDDLIDIECQCFQRVEYVMIKLHVISKDAGQCDNSCDTS 300

Query: 301 GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNISLDVGIGTDDDDLGGQRHYFPNFDEP 360
           GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWN+SLD GIGTDDDDLGGQRHYFPNFDEP
Sbjct: 301 GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNMSLDAGIGTDDDDLGGQRHYFPNFDEP 360

Query: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSF 420
           FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQID ++KHRFHFFNSF
Sbjct: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPQDKHRFHFFNSF 420

Query: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHPSE 480
           FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLF+KDYIFIPINFNLHWSLMVICHP E
Sbjct: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFEKDYIFIPINFNLHWSLMVICHPGE 480

Query: 481 VARCSGKFFIHWAGMRLPSVI 502
           VAR S +       M++P ++
Sbjct: 481 VARYSDEDLT--KSMKVPCIL 499

BLAST of HG10004508 vs. NCBI nr
Match: XP_023546149.1 (probable ubiquitin-like-specific protease 2B isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 890.2 bits (2299), Expect = 8.5e-255
Identity = 439/501 (87.62%), Postives = 461/501 (92.02%), Query Frame = 0

Query: 1   MKNASVKGIEVFDFTEEDELPELISEKRLSKFKNPNLESNAVLKYEFLECDKLTVTNGFV 60
           MKNASVKG+EVFDFTEEDELPELISEKRLSKFKNPNL++NAVLKYEFLECDKLTV NGFV
Sbjct: 1   MKNASVKGLEVFDFTEEDELPELISEKRLSKFKNPNLDTNAVLKYEFLECDKLTVANGFV 60

Query: 61  VEGKEIENPHMDVDLDECNRGCDNGISHHPSVTTKEQQDMEEDKYQLDANTESEVKCHSQ 120
           VEGKEI+NPHMDVDL +CNRGCDNGISH PS TTKEQ  MEE KYQLDANTESE+K HSQ
Sbjct: 61  VEGKEIKNPHMDVDLGKCNRGCDNGISHIPSGTTKEQFIMEEGKYQLDANTESELKSHSQ 120

Query: 121 DMLMQVDNHVTQSLCSELGKIGSSSQSPTLGLNCTLPEFTAERERVDAMSDPNGSMKGSS 180
           DM +QVD HVT SL SEL KIGSSSQSP LGLNCTLPEF AERE +DA+SDPNGSM G+S
Sbjct: 121 DMFVQVDGHVTGSLGSELDKIGSSSQSPILGLNCTLPEFNAEREPIDALSDPNGSMNGNS 180

Query: 181 PMSPPSETVEDGVLLNGKSSDNCSSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240
            MSPPSETVEDGVLLNGK  DNCSSD E DDLN+EVVLYPDYIV GDFYCASPSLTFS N
Sbjct: 181 SMSPPSETVEDGVLLNGKYLDNCSSDNEMDDLNKEVVLYPDYIVYGDFYCASPSLTFSPN 240

Query: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTS 300
            IKINGFADYGSNEFLNLEWRVDDLI IECQCFQRVEYVMIKLHVISKDA +CDN+CDTS
Sbjct: 241 AIKINGFADYGSNEFLNLEWRVDDLIDIECQCFQRVEYVMIKLHVISKDAGQCDNSCDTS 300

Query: 301 GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNISLDVGIGTDDDDLGGQRHYFPNFDEP 360
           GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWN+SLD GIGTDDDDLGGQRHYFPNFDEP
Sbjct: 301 GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNMSLDAGIGTDDDDLGGQRHYFPNFDEP 360

Query: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSF 420
           FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQID ++KHRFHFFNSF
Sbjct: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPQDKHRFHFFNSF 420

Query: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHPSE 480
           FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLF+KDYIFIPINFNLHWSLMVICHP E
Sbjct: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFEKDYIFIPINFNLHWSLMVICHPGE 480

Query: 481 VARCSGKFFIHWAGMRLPSVI 502
           VAR S +       M++P ++
Sbjct: 481 VARYSDEDLT--KSMKVPCIL 499

BLAST of HG10004508 vs. NCBI nr
Match: XP_008444928.1 (PREDICTED: probable ubiquitin-like-specific protease 2B isoform X1 [Cucumis melo])

HSP 1 Score: 887.9 bits (2293), Expect = 4.2e-254
Identity = 438/501 (87.43%), Postives = 457/501 (91.22%), Query Frame = 0

Query: 1   MKNASVKGIEVFDFTEEDELPELISEKRLSKFKNPNLESNAVLKYEFLECDKLTVTNGFV 60
           MKNAS+KG+EVFDFTEEDELPELISEK L+KFKNPNLESNAV KYEFLEC          
Sbjct: 1   MKNASIKGLEVFDFTEEDELPELISEKHLTKFKNPNLESNAVFKYEFLEC---------- 60

Query: 61  VEGKEIENPHMDVDLDECNRGCDNGISHHPSVTTKEQQDMEEDKYQLDANTESEVKCHSQ 120
             GKEIEN HMDVDLDE   GCDNGIS +PS TTKEQQ MEE+KY+ DANTES+V CHSQ
Sbjct: 61  --GKEIENTHMDVDLDERKLGCDNGISCNPSSTTKEQQVMEEEKYRSDANTESKVNCHSQ 120

Query: 121 DMLMQVDNHVTQSLCSELGKIGSSSQSPTLGLNCTLPEFTAERERVDAMSDPNGSMKGSS 180
           DMLMQ+DNHVTQS CSELGKI SSSQSP LGLNCTLPEF AE++ VD +SDPNGSMKGSS
Sbjct: 121 DMLMQIDNHVTQSQCSELGKIVSSSQSPVLGLNCTLPEFPAEQQHVDGLSDPNGSMKGSS 180

Query: 181 PMSPPSETVEDGVLLNGKSSDNCSSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240
           PMSPPSETVEDGVLLNGKSSDNCSSD EKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN
Sbjct: 181 PMSPPSETVEDGVLLNGKSSDNCSSDNEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240

Query: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTS 300
           GIKINGFADYGSNEFLNLEWRVDDLIHIE QCFQRVEYVMIKLHVISKDA ECDNACDTS
Sbjct: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIESQCFQRVEYVMIKLHVISKDAGECDNACDTS 300

Query: 301 GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNISLDVGIGTDDDDLGGQRHYFPNFDEP 360
           GIKEVKIVLVDSYW EKQQKI+SLDSRYMAIWNISLDVG GTDDDDLGGQRHYFPNFDEP
Sbjct: 301 GIKEVKIVLVDSYWPEKQQKIKSLDSRYMAIWNISLDVGTGTDDDDLGGQRHYFPNFDEP 360

Query: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSF 420
           FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQID KEKHRFHFFNSF
Sbjct: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPKEKHRFHFFNSF 420

Query: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHPSE 480
           FFRKLADLDKDPSSASDGRAAFLRVRKWTRKV+LFDKDYIFIPINFNLHWSLMVICHP E
Sbjct: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVNLFDKDYIFIPINFNLHWSLMVICHPGE 480

Query: 481 VARCSGKFFIHWAGMRLPSVI 502
           VARCS +  +    M++P ++
Sbjct: 481 VARCSDEDLM--KSMKVPCIL 487

BLAST of HG10004508 vs. ExPASy Swiss-Prot
Match: Q8L7S0 (Probable ubiquitin-like-specific protease 2B OS=Arabidopsis thaliana OX=3702 GN=ULP2B PE=1 SV=3)

HSP 1 Score: 360.5 bits (924), Expect = 3.1e-98
Identity = 213/519 (41.04%), Postives = 302/519 (58.19%), Query Frame = 0

Query: 7   KGIEVFDFTEEDELPELISEKRLSKFKNPNLESNAVLKYEFLE--CDKLTV--------- 66
           K  EVFDF EEDEL E  + K L KF NP+  ++ VL+ + ++  C++  V         
Sbjct: 3   KNFEVFDFKEEDELAESAAGKLLEKFTNPSPCNSPVLQRQRIQSFCNEKRVEEEEMEGPS 62

Query: 67  -----------------TNGFVVEGKEIE---------NPHM-DVDLDECNRGCDNGISH 126
                             +  V E KE             H+ + D +  N+G   G+  
Sbjct: 63  CAEPATAVESDDHQCEDDSTLVTEAKESRTILTFGLETTDHLEETDAEHVNQGLMLGLK- 122

Query: 127 HPSVTTKEQQDMEEDKYQLDANTESEVKCHSQDMLMQVDNHVTQSLCSELGKIGSSSQSP 186
             +    ++ D++ D + L     SE      D+     +H  +S   +LG  G+S  + 
Sbjct: 123 --TEDLAKETDIDHDNHGLMFGLNSEDDIEETDV-----DHRVESFSCQLG--GNSFYAE 182

Query: 187 TLG-----LNCTLPEFTAERERVDAMSDPNGSMKGSSPMSPPSETVEDGVLLNGKSSDNC 246
           T       LN    + ++  E++D MS  + S+   S +S  S++ +D        +++C
Sbjct: 183 TSSYSQRQLNSPFSDSSSSEEQIDMMSAIDESLSDRSALSEASDSEDDE---EDWMTEHC 242

Query: 247 SSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHNGIKINGFADYGSNEFLNLEWRVD 306
            +D EK DL+  V++  +Y++  D +CA+  + FS NGIKI  F         + E+ V+
Sbjct: 243 FNDEEKIDLSTAVIMTSEYVILKDMHCAASLVIFSCNGIKIKSFLANNEEVPFSCEFGVE 302

Query: 307 DLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTSGIKEVKIVLVDSYWSEKQQKIRS 366
           D++ I+   +Q V  +++++ V+ KD +     C    ++E+KI + +  W  KQQKI S
Sbjct: 303 DIVSIQYNWYQNVGLIILRIRVLLKDEN-----CH-EDMEELKIAVKEHNWPNKQQKINS 362

Query: 367 LDSRYMAIWNISLDVGIGTDDDDLGGQRHYFPNFDEPFEEVVYPKGDPDAVSISKRDVDL 426
           L  +Y A+WN  L+  +     +L  Q+ YFP+FDEPFE+VVYPKGDPDAVSI KRDV+L
Sbjct: 363 LHVKYPAVWNTDLEDDVEVSGYNLNQQKRYFPSFDEPFEDVVYPKGDPDAVSICKRDVEL 422

Query: 427 LQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSFFFRKLADLDKDPSSASDGRAAFL 483
           LQPETFVNDTIIDFYI YLK+QI  +EKHRFHFFNSFFFRKLADLDKDPSS +DG+AAFL
Sbjct: 423 LQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPSSIADGKAAFL 482

BLAST of HG10004508 vs. ExPASy Swiss-Prot
Match: Q0WKV8 (Probable ubiquitin-like-specific protease 2A OS=Arabidopsis thaliana OX=3702 GN=ULP2A PE=2 SV=2)

HSP 1 Score: 264.6 bits (675), Expect = 2.3e-69
Identity = 171/481 (35.55%), Postives = 250/481 (51.98%), Query Frame = 0

Query: 7   KGIEVFDFTEEDELPELISEKRLSKFKNPNLESN--AVLKYEFLECDKLTVTNGFVVEGK 66
           K I+VFD+++ED+  E  S+K L KF +P  + +  A+ KYEFL C              
Sbjct: 13  KPIDVFDYSDEDDRVEEESKKLLRKFDSPVTKKHHCAIDKYEFLRC-------------- 72

Query: 67  EIENPHMDVDLDECNRGCDNGISHHPSVTTKEQQDMEEDKYQLDANTESEVKCHSQDMLM 126
                                                   +  D  +ES+V  H   +++
Sbjct: 73  ----------------------------------------FAKDTQSESKVLQH---IVI 132

Query: 127 QVDNHVTQ--SLCSELGKIGSSSQSPTLGLNCTLPEFTAERERVDAMSDPNGSMKGSSPM 186
            V+  V +  S C EL   G+S                   + +D +S+ +    G   +
Sbjct: 133 DVEVPVKEEPSRC-ELSGDGNS-------------------DLIDVISNGSHRRIGIDSL 192

Query: 187 SPPSETVEDGVLLNGKSSDNCSSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHNGI 246
           +  S +  D V   G++++  S   E D  N +V++ PD I+ GD YC +  LTFS N +
Sbjct: 193 TSSSLSENDEV-STGEATNPASDPHEVDPENAQVLIIPDVIIYGDIYCTNSKLTFSRNCM 252

Query: 247 KINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTSGI 306
            +   +   +    + +W ++D+I IE Q    VE   + + + S+     D A D SGI
Sbjct: 253 NVESSSVNATKGTFSCQWTIEDIIKIESQWCLEVETAFVNVLLKSRKPEGVDIAKDISGI 312

Query: 307 KEVKIVLVDSYWSEKQQKIRSLDSRYMAIW--NISLDVGIGTDDDDLGGQRHYFPNFDEP 366
             +K  + D  WS++ + IRSLDSRY  IW   I+    I     DLG       N  + 
Sbjct: 313 DLLKFSVYDPKWSKEVETIRSLDSRYKNIWFDTITESEEIAFSGHDLGTS---LTNLADS 372

Query: 367 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSF 426
           FE++VYP+G+PDAV + K+D++LL+P  F+NDTIIDFYI+YLK++I  KE+ RFHFFN F
Sbjct: 373 FEDLVYPQGEPDAVVVRKQDIELLKPRRFINDTIIDFYIKYLKNRISPKERGRFHFFNCF 412

Query: 427 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHPSE 482
           FFRKLA+LDK   S   GR A+ RV+KWT+ VDLF+KDYIFIPIN + HWSL++ICHP E
Sbjct: 433 FFRKLANLDKGTPSTCGGREAYQRVQKWTKNVDLFEKDYIFIPINCSFHWSLVIICHPGE 412

BLAST of HG10004508 vs. ExPASy Swiss-Prot
Match: Q2PS26 (Ubiquitin-like-specific protease 1D OS=Arabidopsis thaliana OX=3702 GN=ULP1D PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 3.7e-19
Identity = 50/141 (35.46%), Postives = 85/141 (60.28%), Query Frame = 0

Query: 362 EEVVYP-KGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEK--HRFHFFN 421
           E++ YP + DP  V +  +D++ L P  ++   +++FY+++L+ QI    +     HFFN
Sbjct: 330 EDICYPTRDDPHFVQVCLKDLECLAPREYLTSPVMNFYMRFLQQQISSSNQISADCHFFN 389

Query: 422 SFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHP 481
           ++F++KL+  D      +D  A F+R R+W + +DLF K YIFIPI+ +LHWSL+++C P
Sbjct: 390 TYFYKKLS--DAVTYKGNDKDAFFVRFRRWWKGIDLFRKAYIFIPIHEDLHWSLVIVCIP 449

Query: 482 SEVARCSGKFFIHWAGMRLPS 500
            +    SG   +H   + L S
Sbjct: 450 DKKDE-SGLTILHLDSLGLHS 467

BLAST of HG10004508 vs. ExPASy Swiss-Prot
Match: Q6P7W0 (Sentrin-specific protease 6 OS=Mus musculus OX=10090 GN=Senp6 PE=1 SV=3)

HSP 1 Score: 96.3 bits (238), Expect = 1.1e-18
Identity = 52/142 (36.62%), Postives = 84/142 (59.15%), Query Frame = 0

Query: 343 DDDDLGGQRHYFPNFDEPFEE-VVYPKGD-PDAVSISKRDVDLLQPETFVNDTIIDFYIQ 402
           DDD+  G+ H    F  P E+ +VYP       +S++  D+  L    F+ND IIDFY++
Sbjct: 655 DDDEEAGESHTI--FIGPVEKLIVYPPPPAKGGISVTNEDLHCLSEGEFLNDVIIDFYLK 714

Query: 403 YL-KSQIDLKEKHRFHFFNSFFFRKLADLDK---DPSSASDGRAAFLRVRKWTRKVDLFD 462
           YL   ++  ++  R H F+SFF+++L   ++   + ++ S  +    RV+ WTR VD+F+
Sbjct: 715 YLVLEKLKKEDADRIHIFSSFFYKRLNQRERRNPETTNLSIQQKRHGRVKTWTRHVDIFE 774

Query: 463 KDYIFIPINFNLHWSLMVICHP 479
           KD+IF+P+N   HW L V+C P
Sbjct: 775 KDFIFVPLNEAAHWFLAVVCFP 794

BLAST of HG10004508 vs. ExPASy Swiss-Prot
Match: Q8RWN0 (Ubiquitin-like-specific protease 1C OS=Arabidopsis thaliana OX=3702 GN=ULP1C PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.4e-18
Identity = 51/136 (37.50%), Postives = 79/136 (58.09%), Query Frame = 0

Query: 362 EEVVYPKGDP----DAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEK--HRFH 421
           E++ YP  D     D V +S +D+  L P  ++   +I+FYI+Y++  +   +K     H
Sbjct: 315 EDIYYPSSDQSDGRDLVQVSLKDLKCLSPGEYLTSPVINFYIRYVQHHVFSADKTAANCH 374

Query: 422 FFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVI 481
           FFN+FF++KL +        +D  A F++ R+W +  DLF K YIFIPI+ +LHWSL++I
Sbjct: 375 FFNTFFYKKLTEAVS--YKGNDRDAYFVKFRRWWKGFDLFCKSYIFIPIHEDLHWSLVII 434

Query: 482 CHPSEVARCSGKFFIH 492
           C P +    SG   IH
Sbjct: 435 CIPDKEDE-SGLTIIH 447

BLAST of HG10004508 vs. ExPASy TrEMBL
Match: A0A5A7VH74 (Putative ubiquitin-like-specific protease 2B isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004240 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.5e-260
Identity = 445/498 (89.36%), Postives = 459/498 (92.17%), Query Frame = 0

Query: 1   MKNASVKGIEVFDFTEEDELPELISEKRLSKFKNPNLESNAVLKYEFLECDKLTVTNGFV 60
           MKNAS+KG+EVFDFTEEDELPELISEK L+KFKNPNLESNAV KYEFLEC          
Sbjct: 1   MKNASIKGLEVFDFTEEDELPELISEKHLTKFKNPNLESNAVFKYEFLEC---------- 60

Query: 61  VEGKEIENPHMDVDLDECNRGCDNGISHHPSVTTKEQQDMEEDKYQLDANTESEVKCHSQ 120
             GKEIEN HMDVDLDE   GCDNGIS +PS TTKEQQ MEE+KY+ DANTES+V CHSQ
Sbjct: 61  --GKEIENTHMDVDLDERKLGCDNGISCNPSSTTKEQQVMEEEKYRSDANTESKVNCHSQ 120

Query: 121 DMLMQVDNHVTQSLCSELGKIGSSSQSPTLGLNCTLPEFTAERERVDAMSDPNGSMKGSS 180
           DMLMQ+DNHVTQS CSELGKI SSSQSP LGLNCTLPEF AE++ VD +SDPNGSMKGSS
Sbjct: 121 DMLMQIDNHVTQSQCSELGKIVSSSQSPVLGLNCTLPEFPAEQQHVDGLSDPNGSMKGSS 180

Query: 181 PMSPPSETVEDGVLLNGKSSDNCSSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240
           PMSPPSETVEDGVLLNGKSSDNCSSD EKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN
Sbjct: 181 PMSPPSETVEDGVLLNGKSSDNCSSDNEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240

Query: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTS 300
           GIKINGFADYGSNEFLNLEWRVDDLIHIE QCFQRVEYVMIKLHVISKDA ECDNACDTS
Sbjct: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIESQCFQRVEYVMIKLHVISKDAGECDNACDTS 300

Query: 301 GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNISLDVGIGTDDDDLGGQRHYFPNFDEP 360
           GIKEVKIVLVDSYW EKQQKI+SLDSRYMAIWNISLDVG GTDDDDLGGQRHYFPNFDEP
Sbjct: 301 GIKEVKIVLVDSYWPEKQQKIKSLDSRYMAIWNISLDVGTGTDDDDLGGQRHYFPNFDEP 360

Query: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSF 420
           FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQID KEKHRFHFFNSF
Sbjct: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPKEKHRFHFFNSF 420

Query: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHPSE 480
           FFRKLADLDKDPSSASDGRAAFLRVRKWTRKV+LFDKDYIFIPINFNLHWSLMVICHP E
Sbjct: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVNLFDKDYIFIPINFNLHWSLMVICHPGE 480

Query: 481 VARCSGKFFIHWAGMRLP 499
           VARCSGKFFIHWA +  P
Sbjct: 481 VARCSGKFFIHWANIWSP 486

BLAST of HG10004508 vs. ExPASy TrEMBL
Match: A0A1S3BC96 (probable ubiquitin-like-specific protease 2B isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488127 PE=3 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 2.0e-254
Identity = 438/501 (87.43%), Postives = 457/501 (91.22%), Query Frame = 0

Query: 1   MKNASVKGIEVFDFTEEDELPELISEKRLSKFKNPNLESNAVLKYEFLECDKLTVTNGFV 60
           MKNAS+KG+EVFDFTEEDELPELISEK L+KFKNPNLESNAV KYEFLEC          
Sbjct: 1   MKNASIKGLEVFDFTEEDELPELISEKHLTKFKNPNLESNAVFKYEFLEC---------- 60

Query: 61  VEGKEIENPHMDVDLDECNRGCDNGISHHPSVTTKEQQDMEEDKYQLDANTESEVKCHSQ 120
             GKEIEN HMDVDLDE   GCDNGIS +PS TTKEQQ MEE+KY+ DANTES+V CHSQ
Sbjct: 61  --GKEIENTHMDVDLDERKLGCDNGISCNPSSTTKEQQVMEEEKYRSDANTESKVNCHSQ 120

Query: 121 DMLMQVDNHVTQSLCSELGKIGSSSQSPTLGLNCTLPEFTAERERVDAMSDPNGSMKGSS 180
           DMLMQ+DNHVTQS CSELGKI SSSQSP LGLNCTLPEF AE++ VD +SDPNGSMKGSS
Sbjct: 121 DMLMQIDNHVTQSQCSELGKIVSSSQSPVLGLNCTLPEFPAEQQHVDGLSDPNGSMKGSS 180

Query: 181 PMSPPSETVEDGVLLNGKSSDNCSSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240
           PMSPPSETVEDGVLLNGKSSDNCSSD EKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN
Sbjct: 181 PMSPPSETVEDGVLLNGKSSDNCSSDNEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240

Query: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTS 300
           GIKINGFADYGSNEFLNLEWRVDDLIHIE QCFQRVEYVMIKLHVISKDA ECDNACDTS
Sbjct: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIESQCFQRVEYVMIKLHVISKDAGECDNACDTS 300

Query: 301 GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNISLDVGIGTDDDDLGGQRHYFPNFDEP 360
           GIKEVKIVLVDSYW EKQQKI+SLDSRYMAIWNISLDVG GTDDDDLGGQRHYFPNFDEP
Sbjct: 301 GIKEVKIVLVDSYWPEKQQKIKSLDSRYMAIWNISLDVGTGTDDDDLGGQRHYFPNFDEP 360

Query: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSF 420
           FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQID KEKHRFHFFNSF
Sbjct: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPKEKHRFHFFNSF 420

Query: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHPSE 480
           FFRKLADLDKDPSSASDGRAAFLRVRKWTRKV+LFDKDYIFIPINFNLHWSLMVICHP E
Sbjct: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVNLFDKDYIFIPINFNLHWSLMVICHPGE 480

Query: 481 VARCSGKFFIHWAGMRLPSVI 502
           VARCS +  +    M++P ++
Sbjct: 481 VARCSDEDLM--KSMKVPCIL 487

BLAST of HG10004508 vs. ExPASy TrEMBL
Match: A0A1S3BCD8 (probable ubiquitin-like-specific protease 2B isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488127 PE=3 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 2.0e-254
Identity = 438/501 (87.43%), Postives = 457/501 (91.22%), Query Frame = 0

Query: 1   MKNASVKGIEVFDFTEEDELPELISEKRLSKFKNPNLESNAVLKYEFLECDKLTVTNGFV 60
           MKNAS+KG+EVFDFTEEDELPELISEK L+KFKNPNLESNAV KYEFLEC          
Sbjct: 1   MKNASIKGLEVFDFTEEDELPELISEKHLTKFKNPNLESNAVFKYEFLEC---------- 60

Query: 61  VEGKEIENPHMDVDLDECNRGCDNGISHHPSVTTKEQQDMEEDKYQLDANTESEVKCHSQ 120
             GKEIEN HMDVDLDE   GCDNGIS +PS TTKEQQ MEE+KY+ DANTES+V CHSQ
Sbjct: 61  --GKEIENTHMDVDLDERKLGCDNGISCNPSSTTKEQQVMEEEKYRSDANTESKVNCHSQ 120

Query: 121 DMLMQVDNHVTQSLCSELGKIGSSSQSPTLGLNCTLPEFTAERERVDAMSDPNGSMKGSS 180
           DMLMQ+DNHVTQS CSELGKI SSSQSP LGLNCTLPEF AE++ VD +SDPNGSMKGSS
Sbjct: 121 DMLMQIDNHVTQSQCSELGKIVSSSQSPVLGLNCTLPEFPAEQQHVDGLSDPNGSMKGSS 180

Query: 181 PMSPPSETVEDGVLLNGKSSDNCSSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240
           PMSPPSETVEDGVLLNGKSSDNCSSD EKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN
Sbjct: 181 PMSPPSETVEDGVLLNGKSSDNCSSDNEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240

Query: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTS 300
           GIKINGFADYGSNEFLNLEWRVDDLIHIE QCFQRVEYVMIKLHVISKDA ECDNACDTS
Sbjct: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIESQCFQRVEYVMIKLHVISKDAGECDNACDTS 300

Query: 301 GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNISLDVGIGTDDDDLGGQRHYFPNFDEP 360
           GIKEVKIVLVDSYW EKQQKI+SLDSRYMAIWNISLDVG GTDDDDLGGQRHYFPNFDEP
Sbjct: 301 GIKEVKIVLVDSYWPEKQQKIKSLDSRYMAIWNISLDVGTGTDDDDLGGQRHYFPNFDEP 360

Query: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSF 420
           FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQID KEKHRFHFFNSF
Sbjct: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPKEKHRFHFFNSF 420

Query: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHPSE 480
           FFRKLADLDKDPSSASDGRAAFLRVRKWTRKV+LFDKDYIFIPINFNLHWSLMVICHP E
Sbjct: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVNLFDKDYIFIPINFNLHWSLMVICHPGE 480

Query: 481 VARCSGKFFIHWAGMRLPSVI 502
           VARCS +  +    M++P ++
Sbjct: 481 VARCSDEDLM--KSMKVPCIL 487

BLAST of HG10004508 vs. ExPASy TrEMBL
Match: A0A6J1HC75 (probable ubiquitin-like-specific protease 2B isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462674 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 5.0e-253
Identity = 436/501 (87.03%), Postives = 459/501 (91.62%), Query Frame = 0

Query: 1   MKNASVKGIEVFDFTEEDELPELISEKRLSKFKNPNLESNAVLKYEFLECDKLTVTNGFV 60
           MKNASVKG+EVFDFTEEDELPELISEKRLSKFKNPNL++NAVLKYEFLECDKLTV NGFV
Sbjct: 1   MKNASVKGLEVFDFTEEDELPELISEKRLSKFKNPNLDTNAVLKYEFLECDKLTVANGFV 60

Query: 61  VEGKEIENPHMDVDLDECNRGCDNGISHHPSVTTKEQQDMEEDKYQLDANTESEVKCHSQ 120
           VEGKEI+NPHMDVDL +CNRGCDNGISH PS TTKEQ  MEE KYQLDANTESE+  HSQ
Sbjct: 61  VEGKEIKNPHMDVDLGKCNRGCDNGISHIPSGTTKEQFIMEEGKYQLDANTESELNSHSQ 120

Query: 121 DMLMQVDNHVTQSLCSELGKIGSSSQSPTLGLNCTLPEFTAERERVDAMSDPNGSMKGSS 180
           D+ +QVD HVT SL SEL KIGSSSQSP LGLNCTLPEF AERE +DA+SDPNGSM G+S
Sbjct: 121 DLFVQVDGHVTGSLGSELDKIGSSSQSPILGLNCTLPEFNAEREPIDALSDPNGSMNGNS 180

Query: 181 PMSPPSETVEDGVLLNGKSSDNCSSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240
            MSPPSETVEDGVLLNGK  DNCSSD E DDLN+EVVLYPDYIV GDFYCASPSLTFS N
Sbjct: 181 SMSPPSETVEDGVLLNGKYLDNCSSDNEMDDLNKEVVLYPDYIVYGDFYCASPSLTFSPN 240

Query: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTS 300
            IKINGFADYGSNEFLNLEWRVDDLI IECQCFQRVEYVMIKLHVISKDA +CDN+CDTS
Sbjct: 241 AIKINGFADYGSNEFLNLEWRVDDLIDIECQCFQRVEYVMIKLHVISKDAGQCDNSCDTS 300

Query: 301 GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNISLDVGIGTDDDDLGGQRHYFPNFDEP 360
           GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWN+SLD GIGTDDDDLGGQRHYFPNFDEP
Sbjct: 301 GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNMSLDAGIGTDDDDLGGQRHYFPNFDEP 360

Query: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSF 420
           FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQID ++KHRFHFFNSF
Sbjct: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPQDKHRFHFFNSF 420

Query: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHPSE 480
           FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLF+KDYIFIPINFNLHWSLMVICH  E
Sbjct: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFEKDYIFIPINFNLHWSLMVICHLGE 480

Query: 481 VARCSGKFFIHWAGMRLPSVI 502
           VAR S +       M++P ++
Sbjct: 481 VARYSDEDLT--KSMKVPCIL 499

BLAST of HG10004508 vs. ExPASy TrEMBL
Match: A0A6J1HG30 (probable ubiquitin-like-specific protease 2B isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462674 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 5.0e-253
Identity = 436/501 (87.03%), Postives = 459/501 (91.62%), Query Frame = 0

Query: 1   MKNASVKGIEVFDFTEEDELPELISEKRLSKFKNPNLESNAVLKYEFLECDKLTVTNGFV 60
           MKNASVKG+EVFDFTEEDELPELISEKRLSKFKNPNL++NAVLKYEFLECDKLTV NGFV
Sbjct: 1   MKNASVKGLEVFDFTEEDELPELISEKRLSKFKNPNLDTNAVLKYEFLECDKLTVANGFV 60

Query: 61  VEGKEIENPHMDVDLDECNRGCDNGISHHPSVTTKEQQDMEEDKYQLDANTESEVKCHSQ 120
           VEGKEI+NPHMDVDL +CNRGCDNGISH PS TTKEQ  MEE KYQLDANTESE+  HSQ
Sbjct: 61  VEGKEIKNPHMDVDLGKCNRGCDNGISHIPSGTTKEQFIMEEGKYQLDANTESELNSHSQ 120

Query: 121 DMLMQVDNHVTQSLCSELGKIGSSSQSPTLGLNCTLPEFTAERERVDAMSDPNGSMKGSS 180
           D+ +QVD HVT SL SEL KIGSSSQSP LGLNCTLPEF AERE +DA+SDPNGSM G+S
Sbjct: 121 DLFVQVDGHVTGSLGSELDKIGSSSQSPILGLNCTLPEFNAEREPIDALSDPNGSMNGNS 180

Query: 181 PMSPPSETVEDGVLLNGKSSDNCSSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHN 240
            MSPPSETVEDGVLLNGK  DNCSSD E DDLN+EVVLYPDYIV GDFYCASPSLTFS N
Sbjct: 181 SMSPPSETVEDGVLLNGKYLDNCSSDNEMDDLNKEVVLYPDYIVYGDFYCASPSLTFSPN 240

Query: 241 GIKINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTS 300
            IKINGFADYGSNEFLNLEWRVDDLI IECQCFQRVEYVMIKLHVISKDA +CDN+CDTS
Sbjct: 241 AIKINGFADYGSNEFLNLEWRVDDLIDIECQCFQRVEYVMIKLHVISKDAGQCDNSCDTS 300

Query: 301 GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNISLDVGIGTDDDDLGGQRHYFPNFDEP 360
           GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWN+SLD GIGTDDDDLGGQRHYFPNFDEP
Sbjct: 301 GIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNMSLDAGIGTDDDDLGGQRHYFPNFDEP 360

Query: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSF 420
           FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQID ++KHRFHFFNSF
Sbjct: 361 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDPQDKHRFHFFNSF 420

Query: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHPSE 480
           FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLF+KDYIFIPINFNLHWSLMVICH  E
Sbjct: 421 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFEKDYIFIPINFNLHWSLMVICHLGE 480

Query: 481 VARCSGKFFIHWAGMRLPSVI 502
           VAR S +       M++P ++
Sbjct: 481 VARYSDEDLT--KSMKVPCIL 499

BLAST of HG10004508 vs. TAIR 10
Match: AT1G09730.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 360.5 bits (924), Expect = 2.2e-99
Identity = 213/519 (41.04%), Postives = 302/519 (58.19%), Query Frame = 0

Query: 7   KGIEVFDFTEEDELPELISEKRLSKFKNPNLESNAVLKYEFLE--CDKLTV--------- 66
           K  EVFDF EEDEL E  + K L KF NP+  ++ VL+ + ++  C++  V         
Sbjct: 3   KNFEVFDFKEEDELAESAAGKLLEKFTNPSPCNSPVLQRQRIQSFCNEKRVEEEEMEGPS 62

Query: 67  -----------------TNGFVVEGKEIE---------NPHM-DVDLDECNRGCDNGISH 126
                             +  V E KE             H+ + D +  N+G   G+  
Sbjct: 63  CAEPATAVESDDHQCEDDSTLVTEAKESRTILTFGLETTDHLEETDAEHVNQGLMLGLK- 122

Query: 127 HPSVTTKEQQDMEEDKYQLDANTESEVKCHSQDMLMQVDNHVTQSLCSELGKIGSSSQSP 186
             +    ++ D++ D + L     SE      D+     +H  +S   +LG  G+S  + 
Sbjct: 123 --TEDLAKETDIDHDNHGLMFGLNSEDDIEETDV-----DHRVESFSCQLG--GNSFYAE 182

Query: 187 TLG-----LNCTLPEFTAERERVDAMSDPNGSMKGSSPMSPPSETVEDGVLLNGKSSDNC 246
           T       LN    + ++  E++D MS  + S+   S +S  S++ +D        +++C
Sbjct: 183 TSSYSQRQLNSPFSDSSSSEEQIDMMSAIDESLSDRSALSEASDSEDDE---EDWMTEHC 242

Query: 247 SSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHNGIKINGFADYGSNEFLNLEWRVD 306
            +D EK DL+  V++  +Y++  D +CA+  + FS NGIKI  F         + E+ V+
Sbjct: 243 FNDEEKIDLSTAVIMTSEYVILKDMHCAASLVIFSCNGIKIKSFLANNEEVPFSCEFGVE 302

Query: 307 DLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTSGIKEVKIVLVDSYWSEKQQKIRS 366
           D++ I+   +Q V  +++++ V+ KD +     C    ++E+KI + +  W  KQQKI S
Sbjct: 303 DIVSIQYNWYQNVGLIILRIRVLLKDEN-----CH-EDMEELKIAVKEHNWPNKQQKINS 362

Query: 367 LDSRYMAIWNISLDVGIGTDDDDLGGQRHYFPNFDEPFEEVVYPKGDPDAVSISKRDVDL 426
           L  +Y A+WN  L+  +     +L  Q+ YFP+FDEPFE+VVYPKGDPDAVSI KRDV+L
Sbjct: 363 LHVKYPAVWNTDLEDDVEVSGYNLNQQKRYFPSFDEPFEDVVYPKGDPDAVSICKRDVEL 422

Query: 427 LQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSFFFRKLADLDKDPSSASDGRAAFL 483
           LQPETFVNDTIIDFYI YLK+QI  +EKHRFHFFNSFFFRKLADLDKDPSS +DG+AAFL
Sbjct: 423 LQPETFVNDTIIDFYINYLKNQIQTEEKHRFHFFNSFFFRKLADLDKDPSSIADGKAAFL 482

BLAST of HG10004508 vs. TAIR 10
Match: AT1G09730.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 352.4 bits (903), Expect = 5.9e-97
Identity = 216/548 (39.42%), Postives = 304/548 (55.47%), Query Frame = 0

Query: 7   KGIEVFDFTEEDELPELISEKRLSKFKNPNLESNAVLKYEFLE--CDKLTV--------- 66
           K  EVFDF EEDEL E  + K L KF NP+  ++ VL+ + ++  C++  V         
Sbjct: 3   KNFEVFDFKEEDELAESAAGKLLEKFTNPSPCNSPVLQRQRIQSFCNEKRVEEEEMEGPS 62

Query: 67  -----------------TNGFVVEGKEIE---------NPHM-DVDLDECNRGCDNGISH 126
                             +  V E KE             H+ + D +  N+G   G+  
Sbjct: 63  CAEPATAVESDDHQCEDDSTLVTEAKESRTILTFGLETTDHLEETDAEHVNQGLMLGLK- 122

Query: 127 HPSVTTKEQQDMEEDKYQLDANTESEVKCHSQDMLMQVDNHVTQSLCSELGKIGSSSQSP 186
             +    ++ D++ D + L     SE      D+     +H  +S   +LG  G+S  + 
Sbjct: 123 --TEDLAKETDIDHDNHGLMFGLNSEDDIEETDV-----DHRVESFSCQLG--GNSFYAE 182

Query: 187 TLG-----LNCTLPEFTAERERVDAMSDPNGSMKGSSPMSPPSETVEDGV---------- 246
           T       LN    + ++  E++D MS  + S+   S +S  S++ +D            
Sbjct: 183 TSSYSQRQLNSPFSDSSSSEEQIDMMSAIDESLSDRSALSEASDSEDDEEGTCYTFSLYF 242

Query: 247 ---LLNGKSSD----------------NCSSDTEKDDLNEEVVLYPDYIVCGDFYCASPS 306
              +L    SD                +C +D EK DL+  V++  +Y++  D +CA+  
Sbjct: 243 LIDMLGSPMSDRVLISMLYALKDWMTEHCFNDEEKIDLSTAVIMTSEYVILKDMHCAASL 302

Query: 307 LTFSHNGIKINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDASECD 366
           + FS NGIKI  F         + E+ V+D++ I+   +Q V  +++++ V+ KD +   
Sbjct: 303 VIFSCNGIKIKSFLANNEEVPFSCEFGVEDIVSIQYNWYQNVGLIILRIRVLLKDEN--- 362

Query: 367 NACDTSGIKEVKIVLVDSYWSEKQQKIRSLDSRYMAIWNISLDVGIGTDDDDLGGQRHYF 426
             C    ++E+KI + +  W  KQQKI SL  +Y A+WN  L+  +     +L  Q+ YF
Sbjct: 363 --CH-EDMEELKIAVKEHNWPNKQQKINSLHVKYPAVWNTDLEDDVEVSGYNLNQQKRYF 422

Query: 427 PNFDEPFEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEKHRF 483
           P+FDEPFE+VVYPKGDPDAVSI KRDV+LLQPETFVNDTIIDFYI YLK+QI  +EKHRF
Sbjct: 423 PSFDEPFEDVVYPKGDPDAVSICKRDVELLQPETFVNDTIIDFYINYLKNQIQTEEKHRF 482

BLAST of HG10004508 vs. TAIR 10
Match: AT4G33620.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 264.6 bits (675), Expect = 1.6e-70
Identity = 171/481 (35.55%), Postives = 250/481 (51.98%), Query Frame = 0

Query: 7   KGIEVFDFTEEDELPELISEKRLSKFKNPNLESN--AVLKYEFLECDKLTVTNGFVVEGK 66
           K I+VFD+++ED+  E  S+K L KF +P  + +  A+ KYEFL C              
Sbjct: 13  KPIDVFDYSDEDDRVEEESKKLLRKFDSPVTKKHHCAIDKYEFLRC-------------- 72

Query: 67  EIENPHMDVDLDECNRGCDNGISHHPSVTTKEQQDMEEDKYQLDANTESEVKCHSQDMLM 126
                                                   +  D  +ES+V  H   +++
Sbjct: 73  ----------------------------------------FAKDTQSESKVLQH---IVI 132

Query: 127 QVDNHVTQ--SLCSELGKIGSSSQSPTLGLNCTLPEFTAERERVDAMSDPNGSMKGSSPM 186
            V+  V +  S C EL   G+S                   + +D +S+ +    G   +
Sbjct: 133 DVEVPVKEEPSRC-ELSGDGNS-------------------DLIDVISNGSHRRIGIDSL 192

Query: 187 SPPSETVEDGVLLNGKSSDNCSSDTEKDDLNEEVVLYPDYIVCGDFYCASPSLTFSHNGI 246
           +  S +  D V   G++++  S   E D  N +V++ PD I+ GD YC +  LTFS N +
Sbjct: 193 TSSSLSENDEV-STGEATNPASDPHEVDPENAQVLIIPDVIIYGDIYCTNSKLTFSRNCM 252

Query: 247 KINGFADYGSNEFLNLEWRVDDLIHIECQCFQRVEYVMIKLHVISKDASECDNACDTSGI 306
            +   +   +    + +W ++D+I IE Q    VE   + + + S+     D A D SGI
Sbjct: 253 NVESSSVNATKGTFSCQWTIEDIIKIESQWCLEVETAFVNVLLKSRKPEGVDIAKDISGI 312

Query: 307 KEVKIVLVDSYWSEKQQKIRSLDSRYMAIW--NISLDVGIGTDDDDLGGQRHYFPNFDEP 366
             +K  + D  WS++ + IRSLDSRY  IW   I+    I     DLG       N  + 
Sbjct: 313 DLLKFSVYDPKWSKEVETIRSLDSRYKNIWFDTITESEEIAFSGHDLGTS---LTNLADS 372

Query: 367 FEEVVYPKGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEKHRFHFFNSF 426
           FE++VYP+G+PDAV + K+D++LL+P  F+NDTIIDFYI+YLK++I  KE+ RFHFFN F
Sbjct: 373 FEDLVYPQGEPDAVVVRKQDIELLKPRRFINDTIIDFYIKYLKNRISPKERGRFHFFNCF 412

Query: 427 FFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHPSE 482
           FFRKLA+LDK   S   GR A+ RV+KWT+ VDLF+KDYIFIPIN + HWSL++ICHP E
Sbjct: 433 FFRKLANLDKGTPSTCGGREAYQRVQKWTKNVDLFEKDYIFIPINCSFHWSLVIICHPGE 412

BLAST of HG10004508 vs. TAIR 10
Match: AT1G60220.1 (UB-like protease 1D )

HSP 1 Score: 97.8 bits (242), Expect = 2.6e-20
Identity = 50/141 (35.46%), Postives = 85/141 (60.28%), Query Frame = 0

Query: 362 EEVVYP-KGDPDAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEK--HRFHFFN 421
           E++ YP + DP  V +  +D++ L P  ++   +++FY+++L+ QI    +     HFFN
Sbjct: 330 EDICYPTRDDPHFVQVCLKDLECLAPREYLTSPVMNFYMRFLQQQISSSNQISADCHFFN 389

Query: 422 SFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVICHP 481
           ++F++KL+  D      +D  A F+R R+W + +DLF K YIFIPI+ +LHWSL+++C P
Sbjct: 390 TYFYKKLS--DAVTYKGNDKDAFFVRFRRWWKGIDLFRKAYIFIPIHEDLHWSLVIVCIP 449

Query: 482 SEVARCSGKFFIHWAGMRLPS 500
            +    SG   +H   + L S
Sbjct: 450 DKKDE-SGLTILHLDSLGLHS 467

BLAST of HG10004508 vs. TAIR 10
Match: AT1G10570.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 95.1 bits (235), Expect = 1.7e-19
Identity = 51/136 (37.50%), Postives = 79/136 (58.09%), Query Frame = 0

Query: 362 EEVVYPKGDP----DAVSISKRDVDLLQPETFVNDTIIDFYIQYLKSQIDLKEK--HRFH 421
           E++ YP  D     D V +S +D+  L P  ++   +I+FYI+Y++  +   +K     H
Sbjct: 315 EDIYYPSSDQSDGRDLVQVSLKDLKCLSPGEYLTSPVINFYIRYVQHHVFSADKTAANCH 374

Query: 422 FFNSFFFRKLADLDKDPSSASDGRAAFLRVRKWTRKVDLFDKDYIFIPINFNLHWSLMVI 481
           FFN+FF++KL +        +D  A F++ R+W +  DLF K YIFIPI+ +LHWSL++I
Sbjct: 375 FFNTFFYKKLTEAVS--YKGNDRDAYFVKFRRWWKGFDLFCKSYIFIPIHEDLHWSLVII 434

Query: 482 CHPSEVARCSGKFFIH 492
           C P +    SG   IH
Sbjct: 435 CIPDKEDE-SGLTIIH 447

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886796.13.0e-26089.62probable ubiquitin-like-specific protease 2B [Benincasa hispida][more]
KAA0065091.15.1e-26089.36putative ubiquitin-like-specific protease 2B isoform X1 [Cucumis melo var. makuw... [more]
XP_023546146.18.5e-25587.62probable ubiquitin-like-specific protease 2B isoform X1 [Cucurbita pepo subsp. p... [more]
XP_023546149.18.5e-25587.62probable ubiquitin-like-specific protease 2B isoform X3 [Cucurbita pepo subsp. p... [more]
XP_008444928.14.2e-25487.43PREDICTED: probable ubiquitin-like-specific protease 2B isoform X1 [Cucumis melo... [more]
Match NameE-valueIdentityDescription
Q8L7S03.1e-9841.04Probable ubiquitin-like-specific protease 2B OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q0WKV82.3e-6935.55Probable ubiquitin-like-specific protease 2A OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q2PS263.7e-1935.46Ubiquitin-like-specific protease 1D OS=Arabidopsis thaliana OX=3702 GN=ULP1D PE=... [more]
Q6P7W01.1e-1836.62Sentrin-specific protease 6 OS=Mus musculus OX=10090 GN=Senp6 PE=1 SV=3[more]
Q8RWN02.4e-1837.50Ubiquitin-like-specific protease 1C OS=Arabidopsis thaliana OX=3702 GN=ULP1C PE=... [more]
Match NameE-valueIdentityDescription
A0A5A7VH742.5e-26089.36Putative ubiquitin-like-specific protease 2B isoform X1 OS=Cucumis melo var. mak... [more]
A0A1S3BC962.0e-25487.43probable ubiquitin-like-specific protease 2B isoform X1 OS=Cucumis melo OX=3656 ... [more]
A0A1S3BCD82.0e-25487.43probable ubiquitin-like-specific protease 2B isoform X2 OS=Cucumis melo OX=3656 ... [more]
A0A6J1HC755.0e-25387.03probable ubiquitin-like-specific protease 2B isoform X1 OS=Cucurbita moschata OX... [more]
A0A6J1HG305.0e-25387.03probable ubiquitin-like-specific protease 2B isoform X3 OS=Cucurbita moschata OX... [more]
Match NameE-valueIdentityDescription
AT1G09730.22.2e-9941.04Cysteine proteinases superfamily protein [more]
AT1G09730.15.9e-9739.42Cysteine proteinases superfamily protein [more]
AT4G33620.11.6e-7035.55Cysteine proteinases superfamily protein [more]
AT1G60220.12.6e-2035.46UB-like protease 1D [more]
AT1G10570.11.7e-1937.50Cysteine proteinases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003653Ulp1 protease family, C-terminal catalytic domainPFAMPF02902Peptidase_C48coord: 389..480
e-value: 3.1E-23
score: 82.8
IPR003653Ulp1 protease family, C-terminal catalytic domainPROSITEPS50600ULP_PROTEASEcoord: 374..510
score: 20.933378
NoneNo IPR availableGENE3D3.40.395.10Adenoviral Proteinase; Chain Acoord: 357..491
e-value: 3.8E-36
score: 126.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 169..208
NoneNo IPR availablePANTHERPTHR47764:SF2UBIQUITIN-LIKE-SPECIFIC PROTEASE 2B-RELATEDcoord: 8..485
NoneNo IPR availablePANTHERPTHR47764UBIQUITIN-LIKE-SPECIFIC PROTEASE 2B-RELATEDcoord: 8..485
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 366..480

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10004508.1HG10004508.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008234 cysteine-type peptidase activity