HG10004413 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10004413 vs. NCBI nr
Match: XP_038885119.1 (DNA gyrase subunit A, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 1370.5 bits (3546), Expect = 0.0e+00
Identity = 747/947 (78.88%), Postives = 767/947 (80.99%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFAPPSVSNRYARICHGLSELRFLSTRSSTSSKSIRSVKSRREDG 60
           MASSSGLRISSLLRHQ APPSVSNR+ RICHGLSELRFLST +STSS+SIR VKSRR D 
Sbjct: 1   MASSSGLRISSLLRHQLAPPSVSNRFVRICHGLSELRFLSTANSTSSRSIRLVKSRRRDE 60

Query: 61  PVKEEGDEMQDGNGSVTVKDRGGGDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDG 120
           PVKE G + QDGNGS+TVKDRGG DGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDG
Sbjct: 61  PVKEGGTDGQDGNGSLTVKDRGGSDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDG 120

Query: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180
           LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC
Sbjct: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180

Query: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNT----------------- 240
           PLIQGHGNFGSIDADPPAAMRYTECRLEAL EAMLLSDLELNT                 
Sbjct: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALAEAMLLSDLELNTVDFVPNFDNSQKEPSLL 240

Query: 241 ----------------VGMATNIPPHNLGELVDALCVLIHNPEAT--------------- 300
                           VGMATNIPPHNLGELVDALCVLIHNPEAT               
Sbjct: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIHNPEATLQELLEYMPGPDFPT 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 GGLIMGNNGILEAYRTGRGRVTVRGKIDVELLDLKTKRTAIIIKEIPYQTNKSALVEKIA 360

Query: 361 ------TLDGIGDIRDESDRSGMRIVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVGII 420
                 TLDGI DIRDESDRSGMRIVIELKRGADPLI+QNNL+RLTSLQSSFSCNMVGII
Sbjct: 361 ELVENKTLDGISDIRDESDRSGMRIVIELKRGADPLIIQNNLFRLTSLQSSFSCNMVGII 420

Query: 421 NGQPKLMGLKELLQRDVQGLSYHKHKREDTSLRSVTVSHLPNDIAWVLSQGIVIGLDNLD 480
           NGQPKLMGLKELLQ  +        +R    L               + +GIVIGLDNLD
Sbjct: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLSQAQERR-------HIVEGIVIGLDNLD 480

Query: 481 GVIHLIREASSNSIASANLRTHFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMENI 540
           GVIHLIREASSNSIASA+LRT FNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMENI
Sbjct: 481 GVIHLIREASSNSIASASLRTQFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMENI 540

Query: 541 SKLEELLSSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALS 600
           SKLEELLSSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALS
Sbjct: 541 SKLEELLSSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALS 600

Query: 601 EKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSSRA 660
           EKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYS+RA
Sbjct: 601 EKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSARA 660

Query: 661 YKIPECGRTATGTPLIQILSLSDGERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNFFS 720
           YKIPECGRTATGTPL+QILSLSDGERITSIIPVS FDG+QFLLMLTANGYIKKVSLNFFS
Sbjct: 661 YKIPECGRTATGTPLVQILSLSDGERITSIIPVSEFDGEQFLLMLTANGYIKKVSLNFFS 720

Query: 721 SIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDTIRAQGRNTRGAVAM 780
           +IRTTGIIAIQLVSGDELKWV CCTNDNLVAMASQNGMVILSSCD IR+QGRNTRGAVAM
Sbjct: 721 AIRTTGIIAIQLVSGDELKWVCCCTNDNLVAMASQNGMVILSSCDIIRSQGRNTRGAVAM 780

Query: 781 RLKAGDKMASMDIIPAAVWNNLERMPTTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFVSE 834
           RLKAGDKMASMDIIPAAVWN+LE+MPTTPR                SKG +GPWLLFVSE
Sbjct: 781 RLKAGDKMASMDIIPAAVWNDLEKMPTTPR--------------GSSKGFNGPWLLFVSE 840

BLAST of HG10004413 vs. NCBI nr
Match: XP_022961731.1 (DNA gyrase subunit A, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 1320.1 bits (3415), Expect = 0.0e+00
Identity = 723/948 (76.27%), Postives = 755/948 (79.64%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFAPPSVSNRYARICHGLSELRFLSTRSSTSSKSIRSVKSRREDG 60
           MA SSGLR SSLLRHQFAPP    R+ RI HGLSELRFLST +S SS+SIR VKSRR+D 
Sbjct: 1   MAFSSGLRFSSLLRHQFAPPHAWTRFGRIYHGLSELRFLSTSTSKSSRSIRLVKSRRKDE 60

Query: 61  PVKEEGDEMQDGNGSVTVKDRGGGDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDG 120
            VKEEG   QDGNGSV VKDRGG DGRIVHA LHKEATDAYMAYAMSVLLGRALPDVRDG
Sbjct: 61  SVKEEGVGGQDGNGSVIVKDRGGSDGRIVHALLHKEATDAYMAYAMSVLLGRALPDVRDG 120

Query: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180
           LKPVHRR+LFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC
Sbjct: 121 LKPVHRRVLFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180

Query: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNT----------------- 240
           PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLEL+T                 
Sbjct: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELDTVDFVPNFDSSQKEPSLL 240

Query: 241 ----------------VGMATNIPPHNLGELVDALCVLIHNPEAT--------------- 300
                           VGMATNIPPHNLGELVDALCVLIHNPEAT               
Sbjct: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIHNPEATLQELLEYMPGPDFPT 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 GGLIMGNNGILEAYRTGRGRITVRGKTDIELLDLKTKRTAVIINEIPYQTNKSALVEKIA 360

Query: 361 ------TLDGIGDIRDESDRSGMRIVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVGII 420
                 TLDGI DIRDESDRSGMRIV+ELKRGADPLI+QNNLYRLT+LQSSFSCNMVGII
Sbjct: 361 ELVENKTLDGIADIRDESDRSGMRIVVELKRGADPLIIQNNLYRLTALQSSFSCNMVGII 420

Query: 421 NGQPKLMGLKELLQRDVQ-GLSYHKHKREDTSLRSVTVSHLPNDIAWVLSQGIVIGLDNL 480
           NGQPKLMGLKELLQ  +    S  + +     L++    H        + +GIV GLDNL
Sbjct: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLLQAQERRH--------IVEGIVTGLDNL 480

Query: 481 DGVIHLIREASSNSIASANLRTHFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN 540
           D VI LIREAS NS+ASANLR  F+LSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN
Sbjct: 481 DEVIQLIREASRNSVASANLRAQFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN 540

Query: 541 ISKLEELLSSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLAL 600
           ISKLEELLSSRKNILQ+IEQEAIELKNKFPSPRRSIL+DTDSGQLEDIDVIPN+EMLLAL
Sbjct: 541 ISKLEELLSSRKNILQVIEQEAIELKNKFPSPRRSILKDTDSGQLEDIDVIPNDEMLLAL 600

Query: 601 SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSSR 660
           SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKG VYSSR
Sbjct: 601 SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSSR 660

Query: 661 AYKIPECGRTATGTPLIQILSLSDGERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNFF 720
           AYKIPECGRTATGTP+IQILSLS+GERITSIIPVS F  DQFLLMLTANGYIKKVSLNFF
Sbjct: 661 AYKIPECGRTATGTPIIQILSLSEGERITSIIPVSEFVEDQFLLMLTANGYIKKVSLNFF 720

Query: 721 SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDTIRAQGRNTRGAVA 780
           SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCD IRAQGRNTRGAVA
Sbjct: 721 SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDIIRAQGRNTRGAVA 780

Query: 781 MRLKAGDKMASMDIIPAAVWNNLERMPTTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFVS 834
           MRLKAGDKMASMDIIPA++WN+ ER+PTTPR                +KGS+GPWLLFVS
Sbjct: 781 MRLKAGDKMASMDIIPASMWNDSERIPTTPR--------------SSAKGSNGPWLLFVS 840

BLAST of HG10004413 vs. NCBI nr
Match: XP_023546413.1 (DNA gyrase subunit A, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1318.9 bits (3412), Expect = 0.0e+00
Identity = 723/948 (76.27%), Postives = 757/948 (79.85%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFAPPSVSNRYARICHGLSELRFLSTRSSTSSKSIRSVKSRREDG 60
           MA SSGLR SSLLRHQFAPP  S+R+ARI HGLSELRFLST +S SS+SIR VKSRR+D 
Sbjct: 1   MAFSSGLRFSSLLRHQFAPPHASSRFARIYHGLSELRFLSTSTSKSSRSIRLVKSRRKDE 60

Query: 61  PVKEEGDEMQDGNGSVTVKDRGGGDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDG 120
            VKEEG   QDGNGSV  KDRGG DGRIVHA LHKEATDAYMAYAMSVLLGRALPDVRDG
Sbjct: 61  SVKEEGVGGQDGNGSVIAKDRGGSDGRIVHALLHKEATDAYMAYAMSVLLGRALPDVRDG 120

Query: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180
           LKPVHRR+LFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC
Sbjct: 121 LKPVHRRVLFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180

Query: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNT----------------- 240
           PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLL+DLELNT                 
Sbjct: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDSSQKEPSLL 240

Query: 241 ----------------VGMATNIPPHNLGELVDALCVLIHNPEAT--------------- 300
                           VGMATNIPPHNLGELVDAL VLIHNPEAT               
Sbjct: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALRVLIHNPEATLQELLEYMPGPDFPT 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 GGLIMGINGILEAYRTGRGRITVRGKTDVELLDLKTKRTAVIIKEIPYQTNKSALVEKIA 360

Query: 361 ------TLDGIGDIRDESDRSGMRIVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVGII 420
                 TLDGI DIRDESDRSGMRIV+ELKRG DPLI+QNNLYRLT+LQSSFSCNMVGII
Sbjct: 361 ELVENKTLDGIADIRDESDRSGMRIVVELKRGEDPLIIQNNLYRLTALQSSFSCNMVGII 420

Query: 421 NGQPKLMGLKELLQRDVQ-GLSYHKHKREDTSLRSVTVSHLPNDIAWVLSQGIVIGLDNL 480
           NGQPKLMGLKELLQ  ++   S  + +     L++    H        + +GIVIGLDNL
Sbjct: 421 NGQPKLMGLKELLQAFLEFRCSVVERRARFKLLQAQERRH--------IVEGIVIGLDNL 480

Query: 481 DGVIHLIREASSNSIASANLRTHFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN 540
           D VI LIREASSNS+ASANLR  F+LSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN
Sbjct: 481 DKVIQLIREASSNSVASANLRAQFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN 540

Query: 541 ISKLEELLSSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLAL 600
           ISKLEELLSSRKNILQ+IEQEAIELKNKFPSPRRSIL+DTDSGQLEDIDVIPNEEMLLAL
Sbjct: 541 ISKLEELLSSRKNILQVIEQEAIELKNKFPSPRRSILKDTDSGQLEDIDVIPNEEMLLAL 600

Query: 601 SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSSR 660
           SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKG VYSSR
Sbjct: 601 SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSSR 660

Query: 661 AYKIPECGRTATGTPLIQILSLSDGERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNFF 720
           AYKIPECGRTATGTP+IQILSLS+GERITSIIPVS F  DQFLLMLTANGYIKKVSLNFF
Sbjct: 661 AYKIPECGRTATGTPIIQILSLSEGERITSIIPVSEFVEDQFLLMLTANGYIKKVSLNFF 720

Query: 721 SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDTIRAQGRNTRGAVA 780
           SSIRTTGIIAIQLV+GDELKWVRCCTNDNLVAMASQNGMVILSSCD IRA+GRNTRGAVA
Sbjct: 721 SSIRTTGIIAIQLVNGDELKWVRCCTNDNLVAMASQNGMVILSSCDIIRARGRNTRGAVA 780

Query: 781 MRLKAGDKMASMDIIPAAVWNNLERMPTTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFVS 834
           MRLKAGDKMASMDIIPA+VWN+ ER+PTTP                 +KGS+GPWLLFVS
Sbjct: 781 MRLKAGDKMASMDIIPASVWNDSERIPTTP--------------LSSAKGSNGPWLLFVS 840

BLAST of HG10004413 vs. NCBI nr
Match: KAG6598657.1 (DNA gyrase subunit A, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1317.8 bits (3409), Expect = 0.0e+00
Identity = 722/948 (76.16%), Postives = 754/948 (79.54%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFAPPSVSNRYARICHGLSELRFLSTRSSTSSKSIRSVKSRREDG 60
           MA SSGLR SSLLRHQFAPP    R+ RI HGLSELRFLST +S SS+SIR VKSRR+D 
Sbjct: 1   MAFSSGLRFSSLLRHQFAPPHAWTRFGRIYHGLSELRFLSTSTSKSSRSIRLVKSRRKDE 60

Query: 61  PVKEEGDEMQDGNGSVTVKDRGGGDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDG 120
            VKEEG   QDGNGSV VKDRGG DGRIVHA LHKEATDAYMAYAMSVLLGRALPDVRDG
Sbjct: 61  SVKEEGIGGQDGNGSVIVKDRGGSDGRIVHALLHKEATDAYMAYAMSVLLGRALPDVRDG 120

Query: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180
           LKPVHRR+LFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC
Sbjct: 121 LKPVHRRVLFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180

Query: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNT----------------- 240
           PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLEL+T                 
Sbjct: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELDTVDFVPNFDSSQKEPSLL 240

Query: 241 ----------------VGMATNIPPHNLGELVDALCVLIHNPEAT--------------- 300
                           VGMATNIPPHNLGELVDALCVLIHNPEAT               
Sbjct: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIHNPEATLQELLEYMPGPDFPT 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 GGLIMGNNGILEAYRTGRGRITVRGKTDIELLDLKTKRTAVIINEIPYQTNKSALVEKIA 360

Query: 361 ------TLDGIGDIRDESDRSGMRIVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVGII 420
                 TLDGI DIRDESDRSGMRIV+ELKRGADPLI+QNNLYRLT+LQSSFSCNMVGII
Sbjct: 361 ELVENKTLDGIADIRDESDRSGMRIVVELKRGADPLIIQNNLYRLTALQSSFSCNMVGII 420

Query: 421 NGQPKLMGLKELLQRDVQ-GLSYHKHKREDTSLRSVTVSHLPNDIAWVLSQGIVIGLDNL 480
           NGQPKLMGLKELLQ  +    S  + +     L++    H        + +GIV GLDNL
Sbjct: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLLQAQERRH--------IVEGIVTGLDNL 480

Query: 481 DGVIHLIREASSNSIASANLRTHFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN 540
           D VI LIREAS NS+ASANLR  F+LSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN
Sbjct: 481 DEVIQLIREASRNSVASANLRAQFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN 540

Query: 541 ISKLEELLSSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLAL 600
           ISKLEELLSSRKNILQ+IEQEAIELKNKFPSPRRSIL+DTDSGQLEDIDVIPN+EMLLAL
Sbjct: 541 ISKLEELLSSRKNILQVIEQEAIELKNKFPSPRRSILKDTDSGQLEDIDVIPNDEMLLAL 600

Query: 601 SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSSR 660
           SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKG VYSSR
Sbjct: 601 SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSSR 660

Query: 661 AYKIPECGRTATGTPLIQILSLSDGERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNFF 720
           AYKIPECGRTATGTP+IQILSLS+GERITSIIPVS F  DQFLLMLTANGYIKKVSLNFF
Sbjct: 661 AYKIPECGRTATGTPIIQILSLSEGERITSIIPVSEFVEDQFLLMLTANGYIKKVSLNFF 720

Query: 721 SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDTIRAQGRNTRGAVA 780
           SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCD IRAQGRNTRGAVA
Sbjct: 721 SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDIIRAQGRNTRGAVA 780

Query: 781 MRLKAGDKMASMDIIPAAVWNNLERMPTTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFVS 834
           MRLKAGDKMASMDIIPA++WN+ ER+PTTP                 +KGS+GPWLLFVS
Sbjct: 781 MRLKAGDKMASMDIIPASMWNDSERIPTTP--------------LSSAKGSNGPWLLFVS 840

BLAST of HG10004413 vs. NCBI nr
Match: XP_022997056.1 (DNA gyrase subunit A, chloroplastic/mitochondrial [Cucurbita maxima])

HSP 1 Score: 1311.2 bits (3392), Expect = 0.0e+00
Identity = 720/948 (75.95%), Postives = 753/948 (79.43%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFAPPSVSNRYARICHGLSELRFLSTRSSTSSKSIRSVKSRREDG 60
           MA SSGLR SSLLRHQFAPP  S+R+ARI HGLSELRFLST +S SS+SIR VKSRR+D 
Sbjct: 1   MAFSSGLRFSSLLRHQFAPPHASSRFARIYHGLSELRFLSTSTSKSSRSIRLVKSRRKDE 60

Query: 61  PVKEEGDEMQDGNGSVTVKDRGGGDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDG 120
            VKEEG   QDGNGSV VKDRGG DGRIV AELHKEATDAYMAYAMSVLLGRALPDVRDG
Sbjct: 61  SVKEEGVGGQDGNGSVIVKDRGGSDGRIVQAELHKEATDAYMAYAMSVLLGRALPDVRDG 120

Query: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180
           LKPVHRR+LFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC
Sbjct: 121 LKPVHRRVLFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180

Query: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNT----------------- 240
           PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNT                 
Sbjct: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNTVDFVPNFDSSQKEPSLL 240

Query: 241 ----------------VGMATNIPPHNLGELVDALCVLIHNPEAT--------------- 300
                           VGMATNIPPHNLGELVDALCVLIHNPEAT               
Sbjct: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIHNPEATLQELLEYMPGPDFPT 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 GGLIMGNNGILEAYRTGRGRIIVRGKTDVELLDLKTKQTAVIINEIPYQTNKSALVEKIA 360

Query: 361 ------TLDGIGDIRDESDRSGMRIVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVGII 420
                 TLDGI DIRDESDRSGMRIV+ELKRGADPLI+QNNLYRLT+LQSSFSCNMVGII
Sbjct: 361 ELVENKTLDGIADIRDESDRSGMRIVVELKRGADPLIIQNNLYRLTALQSSFSCNMVGII 420

Query: 421 NGQPKLMGLKELLQRDVQ-GLSYHKHKREDTSLRSVTVSHLPNDIAWVLSQGIVIGLDNL 480
           NGQPKLMGLKELLQ  +    S  + +     L++    H        + +GIV GLDNL
Sbjct: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLLQAQERRH--------IVEGIVTGLDNL 480

Query: 481 DGVIHLIREASSNSIASANLRTHFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN 540
           D VI LIREAS  S+ASA LR  F+LSEKQAEA+LDINL RL+HLERKKF DESKSLM+N
Sbjct: 481 DEVIQLIREASRTSVASAYLRAQFSLSEKQAEALLDINLGRLSHLERKKFTDESKSLMKN 540

Query: 541 ISKLEELLSSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLAL 600
           ISKLEELLSSRKNILQLIEQEAIELKNKF SPRRSIL+DTDSGQLEDIDVIPNEEMLLAL
Sbjct: 541 ISKLEELLSSRKNILQLIEQEAIELKNKFSSPRRSILKDTDSGQLEDIDVIPNEEMLLAL 600

Query: 601 SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSSR 660
           SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKG VYSSR
Sbjct: 601 SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSSR 660

Query: 661 AYKIPECGRTATGTPLIQILSLSDGERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNFF 720
           AYKIPECGRTATGTP+IQILSLS+GERITSIIPVS F  DQFLLMLTANGYIKKVSLNFF
Sbjct: 661 AYKIPECGRTATGTPIIQILSLSEGERITSIIPVSEFVEDQFLLMLTANGYIKKVSLNFF 720

Query: 721 SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDTIRAQGRNTRGAVA 780
           SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCD IRAQGRNTRGAVA
Sbjct: 721 SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDIIRAQGRNTRGAVA 780

Query: 781 MRLKAGDKMASMDIIPAAVWNNLERMPTTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFVS 834
           MRLKAGDKMASMDIIPA+VWN+ ER+PTTP                 SKGS+GPWLLFVS
Sbjct: 781 MRLKAGDKMASMDIIPASVWNDSERIPTTP--------------LSSSKGSNGPWLLFVS 840

BLAST of HG10004413 vs. ExPASy Swiss-Prot
Match: Q5YLB5 (DNA gyrase subunit A, chloroplastic/mitochondrial OS=Nicotiana benthamiana OX=4100 GN=GYRA PE=2 SV=1)

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 608/939 (64.75%), Postives = 685/939 (72.95%), Query Frame = 0

Query: 17  FAPPSVSNRYARICHGLSELRFLSTRSSTSSKSIRSVKSRREDGPVKEEGDEMQDGNGSV 76
           F+     +R++ +    SELRFLS+ +    K +R V +RR++   +E GDE   GNGSV
Sbjct: 20  FSTGITPSRFSGLRKTSSELRFLSSVTPPPRKQLRPVSARRKE---EEVGDE---GNGSV 79

Query: 77  TVKDRG-----GGDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFA 136
            ++DRG      G  R+V  ELHKEAT+AYM+YAMSVLLGRALPDVRDGLKPVHRRIL+A
Sbjct: 80  ILRDRGENEDRNGGERVVLTELHKEATEAYMSYAMSVLLGRALPDVRDGLKPVHRRILYA 139

Query: 137 MHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGS 196
           MHELGLSS+KP+KKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLR PLI+GHGNFGS
Sbjct: 140 MHELGLSSKKPYKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRSPLIRGHGNFGS 199

Query: 197 IDADPPAAMRYTECRLEALTEAMLLSDLELNT---------------------------- 256
           IDADPPAAMRYTECRLEALTE+MLL+DLE NT                            
Sbjct: 200 IDADPPAAMRYTECRLEALTESMLLADLEQNTVDFVPNFDNSQKEPSLLPARVPNLLLNG 259

Query: 257 -----VGMATNIPPHNLGELVDALCVLIHNPEAT-------------------------- 316
                VGMATNIPPHNLGELVDAL  LIHNPEAT                          
Sbjct: 260 ASGIAVGMATNIPPHNLGELVDALSALIHNPEATLQELLEYMPGPDFPTGGIIMGNIGIL 319

Query: 317 -------------------------------------------------------TLDGI 376
                                                                   L+G+
Sbjct: 320 EAFRTGRGRVVIRGKTDIELLDSKTKRAAIIIQEIPYQTNKASLVEKIADLVENKILEGV 379

Query: 377 GDIRDESDRSGMRIVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVGIINGQPKLMGLKE 436
            DIRDESDRSGMRIVIELKRG+DP IV NNLYRLT+LQSSFSCNMVGI+NGQPKLMGLKE
Sbjct: 380 SDIRDESDRSGMRIVIELKRGSDPAIVLNNLYRLTALQSSFSCNMVGILNGQPKLMGLKE 439

Query: 437 LLQRDVQGLSYHKHKREDTSLRSVTVSHLPNDIAWVLSQGIVIGLDNLDGVIHLIREASS 496
           LLQ     L +     E  +   ++ +   N I     +GI++GLDNLD VI+ IR+ASS
Sbjct: 440 LLQ---AFLDFRCSVVERRARFKLSQAQERNHIV----EGIIVGLDNLDEVINTIRKASS 499

Query: 497 NSIASANLRTHFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMENISKLEELLSSRK 556
           N++A+A+LR  F LSEKQAEAILDI+LRRLT LER KF++E KSL   ISKLEELLSS+K
Sbjct: 500 NALAAASLRKEFELSEKQAEAILDISLRRLTALERNKFVEEGKSLRTQISKLEELLSSKK 559

Query: 557 NILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPS 616
            ILQLIE+EAIE+KNKF +PRRS+LEDTDSG LEDIDVIPNEEMLLA+SEKGYVKRMKP 
Sbjct: 560 QILQLIEEEAIEIKNKFFNPRRSMLEDTDSGDLEDIDVIPNEEMLLAISEKGYVKRMKPD 619

Query: 617 TFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSSRAYKIPECGRTAT 676
           TFNLQ+RGTIGKSVGKLRVNDAMSDF+VCRAHD VLYFSDKG VYSS AYKIPEC RTA 
Sbjct: 620 TFNLQNRGTIGKSVGKLRVNDAMSDFLVCRAHDKVLYFSDKGTVYSSPAYKIPECSRTAA 679

Query: 677 GTPLIQILSLSDGERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNFFSSIRTTGIIAIQ 736
           GTPL+QILSLSDGERITSIIPVS F  DQ+L+MLT NGYIKKVSLN+F+SIR TGIIAIQ
Sbjct: 680 GTPLVQILSLSDGERITSIIPVSEFAADQYLVMLTVNGYIKKVSLNYFASIRCTGIIAIQ 739

Query: 737 LVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDTIRAQGRNTRGAVAMRLKAGDKMASM 796
           LV  DELKWV+CC+N++ VAMASQNGMVIL+ C  IRA GRNTRG+VAMRLK GDK+ASM
Sbjct: 740 LVPDDELKWVKCCSNNDFVAMASQNGMVILTPCANIRALGRNTRGSVAMRLKEGDKVASM 799

Query: 797 DIIPAAVWNNLER-MPTTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFVSESGFGKRVPLS 836
           DIIP A+   L++ +    R Y               +   GPWLLFVSESG+GKRVP+S
Sbjct: 800 DIIPDALQKELDKTLEVQQRQY---------------RSMKGPWLLFVSESGYGKRVPVS 859

BLAST of HG10004413 vs. ExPASy Swiss-Prot
Match: Q9CAF6 (DNA gyrase subunit A, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GYRA PE=1 SV=2)

HSP 1 Score: 1023.1 bits (2644), Expect = 1.8e-297
Identity = 582/948 (61.39%), Postives = 667/948 (70.36%), Query Frame = 0

Query: 1   MASSSGLRI-SSLLRHQFAPPSVSNRYARICH-GLSELRFLSTRSSTSSKSIRSVKSRRE 60
           M+ SS LR+ SSLLR  F    +++   R+     S  RF S+R+  S K +     +R 
Sbjct: 19  MSLSSTLRLSSSLLRRSFFRFPLTDPLCRLRRTEPSATRFFSSRTPRSGKFVVGA-GKRG 78

Query: 61  DGPVKEEGDEMQDGNGSVTVKDRGGGDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVR 120
           D  VKEE       NG + V    G + RIV  ELHKEAT++YM+YA+SVLLGRALPDVR
Sbjct: 79  DEQVKEESGA---NNGGLVV---SGDESRIVPFELHKEATESYMSYALSVLLGRALPDVR 138

Query: 121 DGLKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSL 180
           DGLKPVHRRILFAMHELG+SS+KP+KKCARVVGEVLGKFHPHGDTAVYDSLVRMAQ FSL
Sbjct: 139 DGLKPVHRRILFAMHELGMSSKKPYKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQSFSL 198

Query: 181 RCPLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNT--------------- 240
           RCPLIQGHGNFGSIDADPPAAMRYTECRL+ L EA+LLSDL+ +T               
Sbjct: 199 RCPLIQGHGNFGSIDADPPAAMRYTECRLDPLAEAVLLSDLDQDTVDFVANFDNSQKEPA 258

Query: 241 ------------------VGMATNIPPHNLGELVDALCVLIHNPEAT------------- 300
                             VGMATNIPPHNLGELVD LC LIHNPEAT             
Sbjct: 259 VLPARLPALLLNGASGIAVGMATNIPPHNLGELVDVLCALIHNPEATLQELLEYMPAPDF 318

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 319 PTGGIIMGNLGVLDAYRTGRGRVVVRGKAEVELLDPKTKRNAVIITEIPYQTNKATLVQK 378

Query: 361 --------TLDGIGDIRDESDRSGMRIVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVG 420
                   TL+GI DIRDESDR+GMR+VIELKRG DP +V NNLYR T+LQSSFSCNMVG
Sbjct: 379 IAELVENKTLEGISDIRDESDRNGMRVVIELKRGGDPALVLNNLYRHTALQSSFSCNMVG 438

Query: 421 IINGQPKLMGLKELLQRDVQGLSYHKHKREDTSLRSVTVSHLPNDIAWVLSQGIVIGLDN 480
           I +G+PKLMGLKELLQ  +        +R    L     SH        + +GIV+GLDN
Sbjct: 439 ICDGEPKLMGLKELLQAFIDFRCSVVERRARFKL-----SHAQQ--RKHIIEGIVVGLDN 498

Query: 481 LDGVIHLIREASSNSIASANLRTHFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLME 540
           +D VI LI +ASS+S A+A L++ + LSEKQAEAIL+I LRRLT LERKKF DES SL E
Sbjct: 499 VDEVIELITKASSHSSATAALQSEYGLSEKQAEAILEITLRRLTALERKKFTDESSSLTE 558

Query: 541 NISKLEELLSSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLA 600
            I+KLE+LLS+R NIL+LIEQEAIELK++F SPRRS+LED+DSG LEDIDVIPNEEML+A
Sbjct: 559 QITKLEQLLSTRTNILKLIEQEAIELKDRFSSPRRSMLEDSDSGDLEDIDVIPNEEMLMA 618

Query: 601 LSEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSS 660
           +SEKGYVKRMK  TFNLQHRGTIGKSVGKLRV+DAMSDF+VC AHDHVL+FSD+GIVYS+
Sbjct: 619 VSEKGYVKRMKADTFNLQHRGTIGKSVGKLRVDDAMSDFLVCHAHDHVLFFSDRGIVYST 678

Query: 661 RAYKIPECGRTATGTPLIQILSLSDGERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNF 720
           RAYKIPEC R A GTPL+QILS+S+GER+TSI+PVS F  D++LLMLT NG IKKVSL  
Sbjct: 679 RAYKIPECSRNAAGTPLVQILSMSEGERVTSIVPVSEFAEDRYLLMLTVNGCIKKVSLKL 738

Query: 721 FSSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDTIRAQGRNTRGAV 780
           FS IR+TGIIAIQL SGDELKWVRCC++D+LVAMASQNGMV LS+CD +R   RNT+G  
Sbjct: 739 FSGIRSTGIIAIQLNSGDELKWVRCCSSDDLVAMASQNGMVALSTCDGVRTLSRNTKGVT 798

Query: 781 AMRLKAGDKMASMDIIPAAVWNNLERMPTTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFV 833
           AMRLK  DK+ASMDIIPA++  ++E       +                K S+GPWLLFV
Sbjct: 799 AMRLKNEDKIASMDIIPASLRKDMEEKSEDASLV---------------KQSTGPWLLFV 858

BLAST of HG10004413 vs. ExPASy Swiss-Prot
Match: Q7XZF7 (Probable DNA gyrase subunit A, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GYRA PE=2 SV=2)

HSP 1 Score: 947.2 bits (2447), Expect = 1.3e-274
Identity = 534/935 (57.11%), Postives = 643/935 (68.77%), Query Frame = 0

Query: 18  APPSVSNRYARICHGLSELRFLSTRSSTS--SKSIRSVKSRREDGPVKEEGDEMQDGNGS 77
           A  + S R A +   +  +RFLS+ SS+S  S+S++ +++ R+        +    G G 
Sbjct: 25  AAAAASRRRAAVV-AVPAVRFLSSSSSSSDGSRSVQPLRAGRD--------ERAAAGEGG 84

Query: 78  VTVKDRGGGDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHEL 137
             VK+      R+V  ELHKEAT+AYMAYAMSVLLGRALPDVRDGLKPVHRRIL+AMHE+
Sbjct: 85  AAVKE------RVVPVELHKEATEAYMAYAMSVLLGRALPDVRDGLKPVHRRILYAMHEM 144

Query: 138 GLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDAD 197
           GL+SR+PF+KCARVVGEVLGKFHPHGD+AVY++LVRMAQDFS+R PL+QGHGNFGSIDAD
Sbjct: 145 GLASRRPFRKCARVVGEVLGKFHPHGDSAVYETLVRMAQDFSMRYPLVQGHGNFGSIDAD 204

Query: 198 PPAAMRYTECRLE-----------------------------------ALTEAMLLSDLE 257
           PPAAMRYTECRL+                                   A   ++LL+   
Sbjct: 205 PPAAMRYTECRLDVGCFFFLDIDAIFIIGQVDFVPNFDNSQKEPSLLPARVPSLLLNGSS 264

Query: 258 LNTVGMATNIPPHNLGELVDALCVLIHNPEAT---------------------------- 317
              VGMATNIPPHNLGELVD L V+I NPEAT                            
Sbjct: 265 GIAVGMATNIPPHNLGELVDVLSVMIENPEATLQELLECMPGPDFPTGGTIVGNQGILEA 324

Query: 318 -----------------------------------------------------TLDGIGD 377
                                                                 L+GI D
Sbjct: 325 YKTGRGRVVMRGKTDIETIDVKSKRSAIIIKEVPYQTNKSTLVERIAELVEEKVLEGISD 384

Query: 378 IRDESDRSGMRIVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVGIINGQPKLMGLKELL 437
           IRDESDRSGMR+VIELKRGADP IV NNLYR T+LQSSFSCNMV I++GQPKLMGLKE+L
Sbjct: 385 IRDESDRSGMRVVIELKRGADPAIVLNNLYRHTALQSSFSCNMVAILDGQPKLMGLKEIL 444

Query: 438 QRDVQGLSYHKHKREDTSL-RSVTVSHLPNDIAWVLSQGIVIGLDNLDGVIHLIREASSN 497
           Q  +        +R    L +++   H        + +GIVIGLDNLD VI +IR  S++
Sbjct: 445 QAFIDFRCSVIERRARFKLSQALERKH--------IVEGIVIGLDNLDSVIQIIRGTSNH 504

Query: 498 SIASANLRTHFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMENISKLEELLSSRKN 557
           ++A  +L   F LS+KQAEA+LDI LR+LT LERKKF+DE+KSL E ISKL ELLS++K 
Sbjct: 505 AMARESLIKEFGLSDKQAEALLDITLRKLTSLERKKFVDEAKSLSEEISKLNELLSNKKL 564

Query: 558 ILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPST 617
           I QLI QEA +LKNKF +PRRS +ED+ S +++D+D+IPNEEMLL LSEKGYVKRMKP+T
Sbjct: 565 IFQLILQEATDLKNKFATPRRSFIEDSASTEVDDLDIIPNEEMLLILSEKGYVKRMKPNT 624

Query: 618 FNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSSRAYKIPECGRTATG 677
           FNLQ+RGTIGKSVGK+R+ND  SDFIVC+ HDHVLYFSDKGIVYS+RAYKIPEC R ATG
Sbjct: 625 FNLQNRGTIGKSVGKMRMNDNTSDFIVCQTHDHVLYFSDKGIVYSARAYKIPECTRAATG 684

Query: 678 TPLIQILSLSDGERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNFFSSIRTTGIIAIQL 737
           TPL+Q+LSLSDGERITSI+PV+ F  DQ+L+MLT NGYIKKV LN FS+IRT+GII+IQL
Sbjct: 685 TPLVQLLSLSDGERITSIVPVNEFGEDQYLVMLTVNGYIKKVPLNAFSAIRTSGIISIQL 744

Query: 738 VSGDELKWVRCCTNDNLVAMASQNGMVILSSCDTIRAQGRNTRGAVAMRLKAGDKMASMD 797
             GDELKWVR C +D+LVA+ASQNGMVI+++C+ +RA GR TRG +AM+LK GDKMASMD
Sbjct: 745 APGDELKWVRRCGDDDLVALASQNGMVIVNTCNKLRALGRKTRGVLAMKLKEGDKMASMD 804

Query: 798 IIPAAVWNNLERMPTTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFVSESGFGKRVPLSSF 834
           IIPA   N    MP T               +   +  S PWLLF++++G GKRVPL++F
Sbjct: 805 IIPATSHN----MPET---------------YSRVRDLSPPWLLFIADNGIGKRVPLNAF 864

BLAST of HG10004413 vs. ExPASy Swiss-Prot
Match: Q55738 (DNA gyrase subunit A OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=gyrA PE=3 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 9.9e-171
Identity = 359/862 (41.65%), Postives = 489/862 (56.73%), Query Frame = 0

Query: 87  RIVHAELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELGLSSRKPFKKC 146
           R++  +L  E + +Y+ YAMSV++GRALPD RDGLKPVHRRIL+AM+ELGL+  +PF+KC
Sbjct: 7   RLIATDLRNEMSQSYLEYAMSVIVGRALPDARDGLKPVHRRILYAMYELGLTPDRPFRKC 66

Query: 147 ARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADPPAAMRYTECR 206
           ARVVGEVLGK+HPHGDTAVYD+LVRMAQDFS+R PLI GHGNFGS+D DPPAAMRYTE R
Sbjct: 67  ARVVGEVLGKYHPHGDTAVYDALVRMAQDFSMREPLIDGHGNFGSVDNDPPAAMRYTESR 126

Query: 207 LEALTEAMLLSDLELNT---------------------------------VGMATNIPPH 266
           L  L+   LL D+E  T                                 VGMATNIPPH
Sbjct: 127 LRPLSTNSLLRDIEAETVDFIDNFDGSQQEPTVLPARIPQLLINGSSGIAVGMATNIPPH 186

Query: 267 NLGELVDALCVLIHNPEAT----------------------------------------- 326
           NLGE++D    LI NPE T                                         
Sbjct: 187 NLGEVIDGAIALIRNPEITEQELMQIIPGPDFPTGAQILGRSGIREAYLTGRGSITMRGV 246

Query: 327 ------------------------------------------TLDGIGDIRDESDRSGMR 386
                                                      +DGI DIRDESDR GMR
Sbjct: 247 ASIETMEHPGRPDRDAIIVTELPYQTNKAALIERIADLVNDKKIDGIADIRDESDRDGMR 306

Query: 387 IVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVGIINGQPKLMGLKELLQRDVQGLSYHK 446
           IVIELKR A   +V NNLY+ T +QS+F  N++ ++NG P+++ +K+        L+   
Sbjct: 307 IVIELKRDAYARVVLNNLYKQTPIQSNFGANLLALVNGTPEVLTIKKF-------LTVFW 366

Query: 447 HKREDTSLRSVTVSHLPNDIAWVLSQGIVIGLDNLDGVIHLIREASSNSIASANLRTHFN 506
             R +T  R         +    L QG++I LDNLD VI LIR A+  + A   L   F+
Sbjct: 367 EFRIETITRRTRYELRKAEERDHLLQGLLIALDNLDAVIRLIRGAADTASAKTELVEGFS 426

Query: 507 LSEKQAEAILDINLRRLTHLERKKFIDESKSLMENISKLEELLSSRKNILQLIEQEAIEL 566
           LSE QA+AIL + LRRLT LE  K   E   L   I+  +++L+ R+ +  +IE+E  ++
Sbjct: 427 LSEVQADAILQMQLRRLTALEADKITAEHDELQTKIADFQDILARRERVNAIIEEELEQI 486

Query: 567 KNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPSTFNLQHRGTIGKS 626
           K    +PRR+++   D G+L D D+I N++ L+ L+E+GY+KRM  STF  Q+R T GK+
Sbjct: 487 KAIHATPRRTVIVQED-GELIDTDLIANDQALILLTEQGYIKRMPASTFGTQNRATRGKA 546

Query: 627 VGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSSRAYKIPECGRTATGTPLIQILSLSDG 686
             K++ +D +  F+ C  HD VL+FSD+G+VYS  AY+IP   RTA G P++Q+L +   
Sbjct: 547 AAKIKDDDGVEHFLSCCDHDKVLFFSDRGVVYSLNAYQIPIASRTARGVPIVQMLPIPKD 606

Query: 687 ERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNFFSSIRTTGIIAIQLVSGDELKWVRCC 746
           E+ITS++ VS FD D + +MLT  GYIKK +L+ FS+IR  G+IAI LV GD+L+WVR  
Sbjct: 607 EKITSLVSVSEFDDDTYFIMLTKQGYIKKTALSAFSNIRANGLIAISLVEGDQLRWVRLA 666

Query: 747 TNDNLVAMASQNGMVI--LSSCDTIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVWNNL 806
             ++ V + SQ GM I   +  D +RA GR TRG  +MRL++GD + SMDI+P+ V  N+
Sbjct: 667 KAEDSVIIGSQKGMAIHFKADQDELRALGRATRGVKSMRLRSGDALISMDILPSQVVANI 726

Query: 807 ERMPTTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFVSESGFGKRVPLSSFRLYPLNRVGR 830
                      + G     +I E S  + GPWLL V+  GFGKRVP+  FRL     +G 
Sbjct: 727 AVGSEDEPDEDLGGDT--DAILEES-DNPGPWLLGVTMKGFGKRVPIGQFRLQHRAGLGV 786

BLAST of HG10004413 vs. ExPASy Swiss-Prot
Match: P94605 (DNA gyrase subunit A OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=gyrA PE=3 SV=2)

HSP 1 Score: 479.2 bits (1232), Expect = 9.7e-134
Identity = 295/861 (34.26%), Postives = 449/861 (52.15%), Query Frame = 0

Query: 85  DGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELGLSSRKPFK 144
           +G+++  ++  E    Y+ YAMSV++ RALPDVRDGLKPVHRRIL++MHELGL+  K ++
Sbjct: 4   EGKVLPVDISSEMKKCYIDYAMSVIVSRALPDVRDGLKPVHRRILYSMHELGLTPEKGYR 63

Query: 145 KCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADPPAAMRYTE 204
           KCAR+VG+VLGK+HPHGD++VY +LVR+AQDF+LR  ++ GHGNFGS+D D  AAMRYTE
Sbjct: 64  KCARIVGDVLGKYHPHGDSSVYGALVRLAQDFNLRYTVVDGHGNFGSVDGDSAAAMRYTE 123

Query: 205 CRLEALTEAMLLSDLELNT---------------------------------VGMATNIP 264
            ++  +   M + D+  NT                                 VGMATNIP
Sbjct: 124 AKMNKIALEM-VRDIGKNTVDFIPNFDGEEKEPVVLPSRFPNLLVNGSAGIAVGMATNIP 183

Query: 265 PHNLGELVDALCVLIHNPEATTLD------------------------------------ 324
           PHNLGE++D + +LI NPEAT L+                                    
Sbjct: 184 PHNLGEVIDGITMLIDNPEATILELMAQIKGPDFPTAGIIMGKSGIRAAYETGRGKITVR 243

Query: 325 -------------------------------------------GIGDIRDESDRSGMRIV 384
                                                      GI D+RDESDR GMRIV
Sbjct: 244 AKSEIEVEDNGKQKIIITEIPYQVNKARLVESIADLVKDKRIVGISDLRDESDRDGMRIV 303

Query: 385 IELKRGADPLIVQNNLYRLTSLQSSFSCNMVGIINGQPKLMGLKELLQRDVQGLSYHKHK 444
           IE+K+ A+  I+ N LY+ T LQ +F  NM+ +++ +P+++ LK++LQ  ++       +
Sbjct: 304 IEIKKDANSNIILNQLYKHTRLQDTFGINMLALVDNRPEVLNLKQILQHYIKFQEQVIRR 363

Query: 445 REDTSLRSVTVSHLPNDIAWVLSQGIVIGLDNLDGVIHLIREASSNSIASANLRTHFNLS 504
           R +  L   +        A +L +G+ I LD++D VI LIR + +   A   L   F LS
Sbjct: 364 RTEFDLEKASAR------AHIL-EGLKIALDHIDEVISLIRGSKTAQEAKLGLMDKFGLS 423

Query: 505 EKQAEAILDINLRRLTHLERKKFIDESKSLMENISKLEELLSSRKNILQLIEQEAIELKN 564
           EKQA+AILD+ L+RLT LER+K  DE   LM+ I++L+ +L+    IL +I  E  E+K 
Sbjct: 424 EKQAQAILDLKLQRLTGLEREKIEDEYNELMKTIAELKSILADENKILAIIRDELNEIKA 483

Query: 565 KFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPSTFNLQHRGTIGKSVG 624
           K+   R++ +E  ++  ++  D+I  E +++ L+  GY+KR+   T+  Q RG  G    
Sbjct: 484 KYGDERKTAIERAEN-DIDIEDLIQEENVVITLTHAGYIKRINADTYTSQKRGGRGIQAM 543

Query: 625 KLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSSRAYKIPECGRTATGTPLIQILSLSDGER 684
             + +D + +  +   H+++L+F++KG +Y  +AY+IPE GR A GT ++ +L L   E+
Sbjct: 544 TTKEDDFVENIFITSTHNNILFFTNKGRMYKLKAYQIPEAGRAAKGTNVVNLLQLDPNEK 603

Query: 685 ITSIIPVSVFDGDQFLLMLTANGYIKKVSLNFFSSIRTTGIIAIQLVSGDELKWVRCCTN 744
           I ++I +  FD + FL+M T  G IKK  +  + +IR +G+IAI L   DEL  VR    
Sbjct: 604 IQAVISIKEFDEESFLVMCTKKGIIKKTVVGMYKNIRKSGLIAINLNDDDELVSVRITKG 663

Query: 745 DNLVAMASQNGMVILSSCDTIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVWNNLERMP 804
           D+ + + +  G+ I  +   +R  GRN  G   + LK  D +  M++             
Sbjct: 664 DDDIIIVTNKGLAIRFNEVDVRPLGRNALGVKGITLKEDDFVVGMEV------------- 723

Query: 805 TTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFVSESGFGKRVPLSSFRLYPLNRVGRIGYK 834
                                  +    +L VSE+GFGKR  +  ++       G I YK
Sbjct: 724 ----------------------PNQESDVLVVSENGFGKRTHVGEYKCQHRGGKGLITYK 783

BLAST of HG10004413 vs. ExPASy TrEMBL
Match: A0A6J1HB61 (DNA topoisomerase (ATP-hydrolyzing) OS=Cucurbita moschata OX=3662 GN=LOC111462414 PE=3 SV=1)

HSP 1 Score: 1320.1 bits (3415), Expect = 0.0e+00
Identity = 723/948 (76.27%), Postives = 755/948 (79.64%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFAPPSVSNRYARICHGLSELRFLSTRSSTSSKSIRSVKSRREDG 60
           MA SSGLR SSLLRHQFAPP    R+ RI HGLSELRFLST +S SS+SIR VKSRR+D 
Sbjct: 1   MAFSSGLRFSSLLRHQFAPPHAWTRFGRIYHGLSELRFLSTSTSKSSRSIRLVKSRRKDE 60

Query: 61  PVKEEGDEMQDGNGSVTVKDRGGGDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDG 120
            VKEEG   QDGNGSV VKDRGG DGRIVHA LHKEATDAYMAYAMSVLLGRALPDVRDG
Sbjct: 61  SVKEEGVGGQDGNGSVIVKDRGGSDGRIVHALLHKEATDAYMAYAMSVLLGRALPDVRDG 120

Query: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180
           LKPVHRR+LFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC
Sbjct: 121 LKPVHRRVLFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180

Query: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNT----------------- 240
           PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLEL+T                 
Sbjct: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELDTVDFVPNFDSSQKEPSLL 240

Query: 241 ----------------VGMATNIPPHNLGELVDALCVLIHNPEAT--------------- 300
                           VGMATNIPPHNLGELVDALCVLIHNPEAT               
Sbjct: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIHNPEATLQELLEYMPGPDFPT 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 GGLIMGNNGILEAYRTGRGRITVRGKTDIELLDLKTKRTAVIINEIPYQTNKSALVEKIA 360

Query: 361 ------TLDGIGDIRDESDRSGMRIVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVGII 420
                 TLDGI DIRDESDRSGMRIV+ELKRGADPLI+QNNLYRLT+LQSSFSCNMVGII
Sbjct: 361 ELVENKTLDGIADIRDESDRSGMRIVVELKRGADPLIIQNNLYRLTALQSSFSCNMVGII 420

Query: 421 NGQPKLMGLKELLQRDVQ-GLSYHKHKREDTSLRSVTVSHLPNDIAWVLSQGIVIGLDNL 480
           NGQPKLMGLKELLQ  +    S  + +     L++    H        + +GIV GLDNL
Sbjct: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLLQAQERRH--------IVEGIVTGLDNL 480

Query: 481 DGVIHLIREASSNSIASANLRTHFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN 540
           D VI LIREAS NS+ASANLR  F+LSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN
Sbjct: 481 DEVIQLIREASRNSVASANLRAQFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN 540

Query: 541 ISKLEELLSSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLAL 600
           ISKLEELLSSRKNILQ+IEQEAIELKNKFPSPRRSIL+DTDSGQLEDIDVIPN+EMLLAL
Sbjct: 541 ISKLEELLSSRKNILQVIEQEAIELKNKFPSPRRSILKDTDSGQLEDIDVIPNDEMLLAL 600

Query: 601 SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSSR 660
           SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKG VYSSR
Sbjct: 601 SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSSR 660

Query: 661 AYKIPECGRTATGTPLIQILSLSDGERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNFF 720
           AYKIPECGRTATGTP+IQILSLS+GERITSIIPVS F  DQFLLMLTANGYIKKVSLNFF
Sbjct: 661 AYKIPECGRTATGTPIIQILSLSEGERITSIIPVSEFVEDQFLLMLTANGYIKKVSLNFF 720

Query: 721 SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDTIRAQGRNTRGAVA 780
           SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCD IRAQGRNTRGAVA
Sbjct: 721 SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDIIRAQGRNTRGAVA 780

Query: 781 MRLKAGDKMASMDIIPAAVWNNLERMPTTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFVS 834
           MRLKAGDKMASMDIIPA++WN+ ER+PTTPR                +KGS+GPWLLFVS
Sbjct: 781 MRLKAGDKMASMDIIPASMWNDSERIPTTPR--------------SSAKGSNGPWLLFVS 840

BLAST of HG10004413 vs. ExPASy TrEMBL
Match: A0A6J1K6F5 (DNA topoisomerase (ATP-hydrolyzing) OS=Cucurbita maxima OX=3661 GN=LOC111492096 PE=3 SV=1)

HSP 1 Score: 1311.2 bits (3392), Expect = 0.0e+00
Identity = 720/948 (75.95%), Postives = 753/948 (79.43%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFAPPSVSNRYARICHGLSELRFLSTRSSTSSKSIRSVKSRREDG 60
           MA SSGLR SSLLRHQFAPP  S+R+ARI HGLSELRFLST +S SS+SIR VKSRR+D 
Sbjct: 1   MAFSSGLRFSSLLRHQFAPPHASSRFARIYHGLSELRFLSTSTSKSSRSIRLVKSRRKDE 60

Query: 61  PVKEEGDEMQDGNGSVTVKDRGGGDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDG 120
            VKEEG   QDGNGSV VKDRGG DGRIV AELHKEATDAYMAYAMSVLLGRALPDVRDG
Sbjct: 61  SVKEEGVGGQDGNGSVIVKDRGGSDGRIVQAELHKEATDAYMAYAMSVLLGRALPDVRDG 120

Query: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180
           LKPVHRR+LFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC
Sbjct: 121 LKPVHRRVLFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180

Query: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNT----------------- 240
           PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNT                 
Sbjct: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNTVDFVPNFDSSQKEPSLL 240

Query: 241 ----------------VGMATNIPPHNLGELVDALCVLIHNPEAT--------------- 300
                           VGMATNIPPHNLGELVDALCVLIHNPEAT               
Sbjct: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIHNPEATLQELLEYMPGPDFPT 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 GGLIMGNNGILEAYRTGRGRIIVRGKTDVELLDLKTKQTAVIINEIPYQTNKSALVEKIA 360

Query: 361 ------TLDGIGDIRDESDRSGMRIVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVGII 420
                 TLDGI DIRDESDRSGMRIV+ELKRGADPLI+QNNLYRLT+LQSSFSCNMVGII
Sbjct: 361 ELVENKTLDGIADIRDESDRSGMRIVVELKRGADPLIIQNNLYRLTALQSSFSCNMVGII 420

Query: 421 NGQPKLMGLKELLQRDVQ-GLSYHKHKREDTSLRSVTVSHLPNDIAWVLSQGIVIGLDNL 480
           NGQPKLMGLKELLQ  +    S  + +     L++    H        + +GIV GLDNL
Sbjct: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLLQAQERRH--------IVEGIVTGLDNL 480

Query: 481 DGVIHLIREASSNSIASANLRTHFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN 540
           D VI LIREAS  S+ASA LR  F+LSEKQAEA+LDINL RL+HLERKKF DESKSLM+N
Sbjct: 481 DEVIQLIREASRTSVASAYLRAQFSLSEKQAEALLDINLGRLSHLERKKFTDESKSLMKN 540

Query: 541 ISKLEELLSSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLAL 600
           ISKLEELLSSRKNILQLIEQEAIELKNKF SPRRSIL+DTDSGQLEDIDVIPNEEMLLAL
Sbjct: 541 ISKLEELLSSRKNILQLIEQEAIELKNKFSSPRRSILKDTDSGQLEDIDVIPNEEMLLAL 600

Query: 601 SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSSR 660
           SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKG VYSSR
Sbjct: 601 SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSSR 660

Query: 661 AYKIPECGRTATGTPLIQILSLSDGERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNFF 720
           AYKIPECGRTATGTP+IQILSLS+GERITSIIPVS F  DQFLLMLTANGYIKKVSLNFF
Sbjct: 661 AYKIPECGRTATGTPIIQILSLSEGERITSIIPVSEFVEDQFLLMLTANGYIKKVSLNFF 720

Query: 721 SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDTIRAQGRNTRGAVA 780
           SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCD IRAQGRNTRGAVA
Sbjct: 721 SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDIIRAQGRNTRGAVA 780

Query: 781 MRLKAGDKMASMDIIPAAVWNNLERMPTTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFVS 834
           MRLKAGDKMASMDIIPA+VWN+ ER+PTTP                 SKGS+GPWLLFVS
Sbjct: 781 MRLKAGDKMASMDIIPASVWNDSERIPTTP--------------LSSSKGSNGPWLLFVS 840

BLAST of HG10004413 vs. ExPASy TrEMBL
Match: A0A1S3BDB8 (DNA topoisomerase (ATP-hydrolyzing) OS=Cucumis melo OX=3656 GN=LOC103488432 PE=3 SV=1)

HSP 1 Score: 1290.8 bits (3339), Expect = 0.0e+00
Identity = 713/950 (75.05%), Postives = 743/950 (78.21%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFAPPSVSNRYARICHGLSELRFLSTRSSTSSKSIRSVKSRREDG 60
           MASSSGLRIS LLRHQ APP VSNR+ R C GLSELRFL TR+ T+S+S+R  KS R D 
Sbjct: 1   MASSSGLRISYLLRHQLAPPLVSNRFTRTCRGLSELRFLPTRNFTASRSLRLAKSGRRDE 60

Query: 61  PVKEEGDEMQDGNGSVTV-KDRGGGDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRD 120
            VK+EG+E QDGNGSV V KD GG DGRIVHA LHKEATDAYMAYAMSVLLGRALPDVRD
Sbjct: 61  LVKDEGEEGQDGNGSVAVKKDGGGSDGRIVHAALHKEATDAYMAYAMSVLLGRALPDVRD 120

Query: 121 GLKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLR 180
           GLKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLR
Sbjct: 121 GLKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLR 180

Query: 181 CPLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNT---------------- 240
            PLIQGHGNFGS+DADPPAAMRYTECRLEAL+EAMLLSDLE+NT                
Sbjct: 181 SPLIQGHGNFGSVDADPPAAMRYTECRLEALSEAMLLSDLEMNTVDFVPNFDNSQKEPSL 240

Query: 241 -----------------VGMATNIPPHNLGELVDALCVLIHNPEAT-------------- 300
                            VGMATNIPPHNLGE+VDALCVLIHNPEAT              
Sbjct: 241 LPARLPTLLLNGSSGIAVGMATNIPPHNLGEVVDALCVLIHNPEATLQELLEYMPGPDFP 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 TGGLIMGNNGILEAYRTGRGRITVRGKTEVELLDSKTKRTAVIIKEIPYQTNKSALVEKI 360

Query: 361 -------TLDGIGDIRDESDRSGMRIVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVGI 420
                  TLDGI DIRDESDR+GMRIVIELKRGADP I+QNNLYRLTSLQSSFSCNMVGI
Sbjct: 361 AELVENKTLDGISDIRDESDRTGMRIVIELKRGADPSIIQNNLYRLTSLQSSFSCNMVGI 420

Query: 421 INGQPKLMGLKELLQRDVQGLSYHKHKREDTSLRSVTVSHLPNDIAWVLSQGIVIGLDNL 480
           INGQPKLMGLKELLQ  +        +R    L               + +GIVIGLDNL
Sbjct: 421 INGQPKLMGLKELLQAFLDFRCSVVERRARFKLSQAQERR-------HIVEGIVIGLDNL 480

Query: 481 DGVIHLIREASSNSIASANLRTHFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEN 540
           DGVI LIREASS+SIASA+LRT FNLSEKQAEA+LDINLRRLTHLERKKF DESKSL EN
Sbjct: 481 DGVIRLIREASSHSIASASLRTQFNLSEKQAEAVLDINLRRLTHLERKKFTDESKSLTEN 540

Query: 541 ISKLEELLSSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLAL 600
           ISKLEELLSSRKNILQLIEQEA ELKNKFPSPRRS+LEDTDSGQLEDIDVIPNEEMLLAL
Sbjct: 541 ISKLEELLSSRKNILQLIEQEATELKNKFPSPRRSVLEDTDSGQLEDIDVIPNEEMLLAL 600

Query: 601 SEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSSR 660
           SEKGYVKRMKP+TF+LQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYS+R
Sbjct: 601 SEKGYVKRMKPNTFSLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSAR 660

Query: 661 AYKIPECGRTATGTPLIQILSLSDGERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNFF 720
           AYKIPECGRTA GTPL+QILSLSDGERITSIIPVS F+ DQFLLMLTA GYIKKVSLNFF
Sbjct: 661 AYKIPECGRTAAGTPLVQILSLSDGERITSIIPVSEFNEDQFLLMLTAYGYIKKVSLNFF 720

Query: 721 SSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDTIRAQGRNTRGAVA 780
           SSIRTTGIIAIQLVSGDELKWVR CTNDNLVAMASQNGMVILSSCDT+RA GRNTRGAVA
Sbjct: 721 SSIRTTGIIAIQLVSGDELKWVRRCTNDNLVAMASQNGMVILSSCDTVRALGRNTRGAVA 780

Query: 781 MRLKAGDKMASMDIIPAAVWNNLERMPTTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFVS 836
           MRLKAGDKMASMDIIPAAVWN+LER                      SK S+GPWLLFVS
Sbjct: 781 MRLKAGDKMASMDIIPAAVWNDLER--------------------NSSKISNGPWLLFVS 840

BLAST of HG10004413 vs. ExPASy TrEMBL
Match: A0A6J1BTL3 (DNA topoisomerase (ATP-hydrolyzing) OS=Momordica charantia OX=3673 GN=LOC111004692 PE=3 SV=1)

HSP 1 Score: 1288.5 bits (3333), Expect = 0.0e+00
Identity = 713/947 (75.29%), Postives = 740/947 (78.14%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFAPPSVSNRYARICHGLSELRFLSTRSSTSSKSIRSVKSRREDG 60
           MASSSGLRISSLLRHQF PPSVSNR+ RICHGLSELRFL T +STS +SIR + SR    
Sbjct: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSTSTSFRSIRLLSSR---- 60

Query: 61  PVKEEGDEMQDGNGSVTVKDRGGGDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDG 120
                G E+  G G        GG+GR+VH ELHKEATDAYMAYAMSVLLGRALPDVRDG
Sbjct: 61  -----GGEVGGGGGD-------GGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDG 120

Query: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180
           LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC
Sbjct: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180

Query: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNT----------------- 240
           PLIQGHGNFGSIDADP AAMRYTECRLEALTEAMLL+DLELNT                 
Sbjct: 181 PLIQGHGNFGSIDADPAAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLL 240

Query: 241 ----------------VGMATNIPPHNLGELVDALCVLIHNPEAT--------------- 300
                           VGMATNIPPHNLGELVDALCVLI NPEAT               
Sbjct: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPT 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 GGLIMGNNGILEAYRTGRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIA 360

Query: 361 ------TLDGIGDIRDESDRSGMRIVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVGII 420
                 TLDG+ DIRDESDRSGMRIVIELKRGADPLI+QNNLYRLTSLQS FSCNMVGII
Sbjct: 361 ELVENKTLDGVSDIRDESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGII 420

Query: 421 NGQPKLMGLKELLQRDVQGLSYHKHKREDTSLRSVTVSHLPNDIAWVLSQGIVIGLDNLD 480
           NGQPKLMGLKELLQ  +        +R    L+              + +GIVIGLDNLD
Sbjct: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLKQAKERR-------HIVEGIVIGLDNLD 480

Query: 481 GVIHLIREASSNSIASANLRTHFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMENI 540
           GVI+LIREASSNSIASANLRT F+LSEKQAEAILDINLRRLTHLERKKFIDESKSLME+I
Sbjct: 481 GVINLIREASSNSIASANLRTEFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHI 540

Query: 541 SKLEELLSSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALS 600
           SKL+ELLSSRKNILQLIEQEAIELK+KFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALS
Sbjct: 541 SKLDELLSSRKNILQLIEQEAIELKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALS 600

Query: 601 EKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSSRA 660
           EKGYVKRMKP+TFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKG VYS+RA
Sbjct: 601 EKGYVKRMKPNTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARA 660

Query: 661 YKIPECGRTATGTPLIQILSLSDGERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNFFS 720
           YKIPEC RTATGTPL+QILSLSDGERITSIIPVS FDGDQFLLMLTANGYIKKVSLN FS
Sbjct: 661 YKIPECARTATGTPLVQILSLSDGERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFS 720

Query: 721 SIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDTIRAQGRNTRGAVAM 780
           SIR+TGIIAIQLVSGDELKWVR C ND+L+AMASQNGMVILSSCD IRAQGRNTRGAVAM
Sbjct: 721 SIRSTGIIAIQLVSGDELKWVRRCANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAM 780

Query: 781 RLKAGDKMASMDIIPAAVWNNLERMPTTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFVSE 834
           RLKAGDKMASMDIIPAAV NNLERMPTTPR                SKG +GPWLLFVSE
Sbjct: 781 RLKAGDKMASMDIIPAAVRNNLERMPTTPR--------------SSSKGFNGPWLLFVSE 840

BLAST of HG10004413 vs. ExPASy TrEMBL
Match: A0A6J1BR81 (DNA topoisomerase (ATP-hydrolyzing) OS=Momordica charantia OX=3673 GN=LOC111004692 PE=3 SV=1)

HSP 1 Score: 1287.3 bits (3330), Expect = 0.0e+00
Identity = 715/949 (75.34%), Postives = 742/949 (78.19%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFAPPSVSNRYARICHGLSELRFLSTRSSTSSKSIRSVKSRREDG 60
           MASSSGLRISSLLRHQF PPSVSNR+ RICHGLSELRFL T +STS +SIR + SR    
Sbjct: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSTSTSFRSIRLLSSR---- 60

Query: 61  PVKEEGDEMQDGNGSVTVKDRGGGDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDG 120
                G E+  G G        GG+GR+VH ELHKEATDAYMAYAMSVLLGRALPDVRDG
Sbjct: 61  -----GGEVGGGGGD-------GGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDG 120

Query: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180
           LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC
Sbjct: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180

Query: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNT----------------- 240
           PLIQGHGNFGSIDADP AAMRYTECRLEALTEAMLL+DLELNT                 
Sbjct: 181 PLIQGHGNFGSIDADPAAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLL 240

Query: 241 ----------------VGMATNIPPHNLGELVDALCVLIHNPEAT--------------- 300
                           VGMATNIPPHNLGELVDALCVLI NPEAT               
Sbjct: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPT 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 GGLIMGNNGILEAYRTGRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIA 360

Query: 361 ------TLDGIGDIRDESDRSGMRIVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVGII 420
                 TLDG+ DIRDESDRSGMRIVIELKRGADPLI+QNNLYRLTSLQS FSCNMVGII
Sbjct: 361 ELVENKTLDGVSDIRDESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGII 420

Query: 421 NGQPKLMGLKELLQRDVQGLSYHKHKREDTSLRSVTVSHLPNDIAWVLSQGIVIGLDNLD 480
           NGQPKLMGLKELLQ  +        +R    L+              + +GIVIGLDNLD
Sbjct: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLKQAKERR-------HIVEGIVIGLDNLD 480

Query: 481 GVIHLIREASSNSIASANLRTHFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMENI 540
           GVI+LIREASSNSIASANLRT F+LSEKQAEAILDINLRRLTHLERKKFIDESKSLME+I
Sbjct: 481 GVINLIREASSNSIASANLRTEFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHI 540

Query: 541 SKLEELLSSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALS 600
           SKL+ELLSSRKNILQLIEQEAIELK+KFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALS
Sbjct: 541 SKLDELLSSRKNILQLIEQEAIELKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALS 600

Query: 601 EKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSSRA 660
           EKGYVKRMKP+TFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKG VYS+RA
Sbjct: 601 EKGYVKRMKPNTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARA 660

Query: 661 YKIPECGRTATGTPLIQILSLSDGERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNFFS 720
           YKIPEC RTATGTPL+QILSLSDGERITSIIPVS FDGDQFLLMLTANGYIKKVSLN FS
Sbjct: 661 YKIPECARTATGTPLVQILSLSDGERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFS 720

Query: 721 SIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDTIRAQGRNTRGAVAM 780
           SIR+TGIIAIQLVSGDELKWVR C ND+L+AMASQNGMVILSSCD IRAQGRNTRGAVAM
Sbjct: 721 SIRSTGIIAIQLVSGDELKWVRRCANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAM 780

Query: 781 RLKAGDKMASMDIIPAAVWNNLERMPTTPRIYPVVGYKLYLSIF--EYSKGSSGPWLLFV 834
           RLKAGDKMASMDIIPAAV NNLERMPTTPR           S F    SKG +GPWLLFV
Sbjct: 781 RLKAGDKMASMDIIPAAVRNNLERMPTTPR-----------SSFGVSSSKGFNGPWLLFV 840

BLAST of HG10004413 vs. TAIR 10
Match: AT3G10690.1 (DNA GYRASE A )

HSP 1 Score: 1023.1 bits (2644), Expect = 1.3e-298
Identity = 582/948 (61.39%), Postives = 667/948 (70.36%), Query Frame = 0

Query: 1   MASSSGLRI-SSLLRHQFAPPSVSNRYARICH-GLSELRFLSTRSSTSSKSIRSVKSRRE 60
           M+ SS LR+ SSLLR  F    +++   R+     S  RF S+R+  S K +     +R 
Sbjct: 19  MSLSSTLRLSSSLLRRSFFRFPLTDPLCRLRRTEPSATRFFSSRTPRSGKFVVGA-GKRG 78

Query: 61  DGPVKEEGDEMQDGNGSVTVKDRGGGDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVR 120
           D  VKEE       NG + V    G + RIV  ELHKEAT++YM+YA+SVLLGRALPDVR
Sbjct: 79  DEQVKEESGA---NNGGLVV---SGDESRIVPFELHKEATESYMSYALSVLLGRALPDVR 138

Query: 121 DGLKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSL 180
           DGLKPVHRRILFAMHELG+SS+KP+KKCARVVGEVLGKFHPHGDTAVYDSLVRMAQ FSL
Sbjct: 139 DGLKPVHRRILFAMHELGMSSKKPYKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQSFSL 198

Query: 181 RCPLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNT--------------- 240
           RCPLIQGHGNFGSIDADPPAAMRYTECRL+ L EA+LLSDL+ +T               
Sbjct: 199 RCPLIQGHGNFGSIDADPPAAMRYTECRLDPLAEAVLLSDLDQDTVDFVANFDNSQKEPA 258

Query: 241 ------------------VGMATNIPPHNLGELVDALCVLIHNPEAT------------- 300
                             VGMATNIPPHNLGELVD LC LIHNPEAT             
Sbjct: 259 VLPARLPALLLNGASGIAVGMATNIPPHNLGELVDVLCALIHNPEATLQELLEYMPAPDF 318

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 319 PTGGIIMGNLGVLDAYRTGRGRVVVRGKAEVELLDPKTKRNAVIITEIPYQTNKATLVQK 378

Query: 361 --------TLDGIGDIRDESDRSGMRIVIELKRGADPLIVQNNLYRLTSLQSSFSCNMVG 420
                   TL+GI DIRDESDR+GMR+VIELKRG DP +V NNLYR T+LQSSFSCNMVG
Sbjct: 379 IAELVENKTLEGISDIRDESDRNGMRVVIELKRGGDPALVLNNLYRHTALQSSFSCNMVG 438

Query: 421 IINGQPKLMGLKELLQRDVQGLSYHKHKREDTSLRSVTVSHLPNDIAWVLSQGIVIGLDN 480
           I +G+PKLMGLKELLQ  +        +R    L     SH        + +GIV+GLDN
Sbjct: 439 ICDGEPKLMGLKELLQAFIDFRCSVVERRARFKL-----SHAQQ--RKHIIEGIVVGLDN 498

Query: 481 LDGVIHLIREASSNSIASANLRTHFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLME 540
           +D VI LI +ASS+S A+A L++ + LSEKQAEAIL+I LRRLT LERKKF DES SL E
Sbjct: 499 VDEVIELITKASSHSSATAALQSEYGLSEKQAEAILEITLRRLTALERKKFTDESSSLTE 558

Query: 541 NISKLEELLSSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLA 600
            I+KLE+LLS+R NIL+LIEQEAIELK++F SPRRS+LED+DSG LEDIDVIPNEEML+A
Sbjct: 559 QITKLEQLLSTRTNILKLIEQEAIELKDRFSSPRRSMLEDSDSGDLEDIDVIPNEEMLMA 618

Query: 601 LSEKGYVKRMKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSS 660
           +SEKGYVKRMK  TFNLQHRGTIGKSVGKLRV+DAMSDF+VC AHDHVL+FSD+GIVYS+
Sbjct: 619 VSEKGYVKRMKADTFNLQHRGTIGKSVGKLRVDDAMSDFLVCHAHDHVLFFSDRGIVYST 678

Query: 661 RAYKIPECGRTATGTPLIQILSLSDGERITSIIPVSVFDGDQFLLMLTANGYIKKVSLNF 720
           RAYKIPEC R A GTPL+QILS+S+GER+TSI+PVS F  D++LLMLT NG IKKVSL  
Sbjct: 679 RAYKIPECSRNAAGTPLVQILSMSEGERVTSIVPVSEFAEDRYLLMLTVNGCIKKVSLKL 738

Query: 721 FSSIRTTGIIAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDTIRAQGRNTRGAV 780
           FS IR+TGIIAIQL SGDELKWVRCC++D+LVAMASQNGMV LS+CD +R   RNT+G  
Sbjct: 739 FSGIRSTGIIAIQLNSGDELKWVRCCSSDDLVAMASQNGMVALSTCDGVRTLSRNTKGVT 798

Query: 781 AMRLKAGDKMASMDIIPAAVWNNLERMPTTPRIYPVVGYKLYLSIFEYSKGSSGPWLLFV 833
           AMRLK  DK+ASMDIIPA++  ++E       +                K S+GPWLLFV
Sbjct: 799 AMRLKNEDKIASMDIIPASLRKDMEEKSEDASLV---------------KQSTGPWLLFV 858

BLAST of HG10004413 vs. TAIR 10
Match: AT3G23890.1 (topoisomerase II )

HSP 1 Score: 51.6 bits (122), Expect = 3.5e-06
Identity = 39/118 (33.05%), Postives = 63/118 (53.39%), Query Frame = 0

Query: 110 LGRALPDVRDGLKPVHRRILFAMHELGLSSRKPFKKCARVVGEV--LGKFHPHGDTAVYD 169
           L R++P + DGLKP  R+ILF   +    +RK   K A++VG V  L  +H HG+ ++  
Sbjct: 700 LQRSIPSMVDGLKPGQRKILFVAFK--KIARKEM-KVAQLVGYVSLLSAYH-HGEQSLAS 759

Query: 170 SLVRMAQDF--SLRCPLIQGHGNFG--SIDADPPAAMRYTECRLEALTEAMLLSDLEL 222
           +++ MAQD+  S    L+  +G FG  +      A+ RY   +L  +T  +   D +L
Sbjct: 760 AIIGMAQDYVGSNNINLLLPNGQFGTRTSGGKDSASARYIFTKLSPVTRILFPKDDDL 813

BLAST of HG10004413 vs. TAIR 10
Match: AT3G23890.2 (topoisomerase II )

HSP 1 Score: 51.6 bits (122), Expect = 3.5e-06
Identity = 39/118 (33.05%), Postives = 63/118 (53.39%), Query Frame = 0

Query: 110 LGRALPDVRDGLKPVHRRILFAMHELGLSSRKPFKKCARVVGEV--LGKFHPHGDTAVYD 169
           L R++P + DGLKP  R+ILF   +    +RK   K A++VG V  L  +H HG+ ++  
Sbjct: 700 LQRSIPSMVDGLKPGQRKILFVAFK--KIARKEM-KVAQLVGYVSLLSAYH-HGEQSLAS 759

Query: 170 SLVRMAQDF--SLRCPLIQGHGNFG--SIDADPPAAMRYTECRLEALTEAMLLSDLEL 222
           +++ MAQD+  S    L+  +G FG  +      A+ RY   +L  +T  +   D +L
Sbjct: 760 AIIGMAQDYVGSNNINLLLPNGQFGTRTSGGKDSASARYIFTKLSPVTRILFPKDDDL 813