HG10004407 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10004407
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionAlpha/beta-Hydrolases superfamily protein
LocationChr08: 16688672 .. 16689982 (-)
RNA-Seq ExpressionHG10004407
SyntenyHG10004407
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAATAAAAACTATAAAACAAATAGGGTAAGTTGTTAGAAACTTTGCAACTTTATTTATTTATTAATGTTTTTCATGGAATCGAATTTTGAAAGAAATATAATATAACTTTAAATTACAAATTTGCAAATTTAAAATTCGTAATAAAGTTGCAAGACATCTGACGTAGGGTGAAGTGACGAAGATGACAACGACAAACCGGATTTTGTAAAGTTTCAGTGATTTTCCGACGAACTAACCTGCAAATTGCGTCTTTAATTTCCTCTTTCTTCCCTCTCATTCGATTCCATTTCCCAGTTGTTATATAAACCAACCCATTTTTCCTAATTTCTCTCCATCATCTTTCTTCTTCTTTGACGACGGCTCCGTCGACCGGACCTGGACCGGACCGCCGGAAGTCAAGTTCGTCGCGGAGTCTGTCCCACCTCACGACGACTTCATCGACGGAGTCGCCGTTCGTGACTTGGTGATTGATCAGAACTCTGGTCTCCGTGTTCGAATCTATTTACCAGAGGTGAAATGCAGCGGCGGAAAGAAACTGCTGGTTGTCATTCATTTTCACGGCGGTGGATTTTGTATTAGCGAAGCGGATTGGTATATGTATTATCATACTTACACAAACCTTGTGAGATCCGCCGAAGCAATTTGTGTCTCTGTTTACCTCCGTCGAGCGCCAGAGCATCGTCTTCCAGCGGCGATCGACGACGGCTTCTCCGCTCTCAAGTGGTTGCAATCGGTGGCTCTCGGTAACGAAACCGAGCCTTGGATTGTCGAAAACGCGGATTTCAATCGCGTTTTCCTAATCGGAGATAGCTCTGGCGGAAATCTCGTTCACTCTGTTGCAGCCCTGGCCGGAGAAATCGATCTCACTCCTTTGAAACTTGCCGGTGGAATTCCGATTCATCCTGGTTTTGTCAGAGCAAAACGAAGCAAATCAGAGATTGAGAATCCGCAATCGCCGTTCTTGACTTTGGATATGGTAGACAAATTTCTGAGCCTCTCATTACCGGTGGGGAGTACAAAGGATCATCCGATTACGTGTCCGATGGGAATCGACGCGCTGCCCCTTGAAAAGCTAAATCTGCCGCCATTTCTACTGTGCATTGCAGAGAAAGATCTGATAATCGACACCGAAATGGAGTATTACGAAGCAATGAAGGCGGCGAATAAAGAAGTGGAGATTTTAATGAGCAAAGGAATGGGACATAGCTTTTATCTGAACAAGATCGCCATGAAAATGGACCCTGAAACAGCTGCTGAATCGGACCGCCTCTTTGCCGGAATTGCTCGATTTATCAAGCAACATTAG

mRNA sequence

ATGATAATAAAAACTATAAAACAAATAGGTTGTTATATAAACCAACCCATTTTTCCTAATTTCTCTCCATCATCTTTCTTCTTCTTTGACGACGGCTCCGTCGACCGGACCTGGACCGGACCGCCGGAAGTCAAGTTCGTCGCGGAGTCTGTCCCACCTCACGACGACTTCATCGACGGAGTCGCCGTTCGTGACTTGGTGATTGATCAGAACTCTGGTCTCCGTGTTCGAATCTATTTACCAGAGGTGAAATGCAGCGGCGGAAAGAAACTGCTGGTTGTCATTCATTTTCACGGCGGTGGATTTTGTATTAGCGAAGCGGATTGGTATATGTATTATCATACTTACACAAACCTTGTGAGATCCGCCGAAGCAATTTGTGTCTCTGTTTACCTCCGTCGAGCGCCAGAGCATCGTCTTCCAGCGGCGATCGACGACGGCTTCTCCGCTCTCAAGTGGTTGCAATCGGTGGCTCTCGGTAACGAAACCGAGCCTTGGATTGTCGAAAACGCGGATTTCAATCGCGTTTTCCTAATCGGAGATAGCTCTGGCGGAAATCTCGTTCACTCTGTTGCAGCCCTGGCCGGAGAAATCGATCTCACTCCTTTGAAACTTGCCGGTGGAATTCCGATTCATCCTGGTTTTGTCAGAGCAAAACGAAGCAAATCAGAGATTGAGAATCCGCAATCGCCGTTCTTGACTTTGGATATGGTAGACAAATTTCTGAGCCTCTCATTACCGGTGGGGAGTACAAAGGATCATCCGATTACGTGTCCGATGGGAATCGACGCGCTGCCCCTTGAAAAGCTAAATCTGCCGCCATTTCTACTGTGCATTGCAGAGAAAGATCTGATAATCGACACCGAAATGGAGTATTACGAAGCAATGAAGGCGGCGAATAAAGAAGTGGAGATTTTAATGAGCAAAGGAATGGGACATAGCTTTTATCTGAACAAGATCGCCATGAAAATGGACCCTGAAACAGCTGCTGAATCGGACCGCCTCTTTGCCGGAATTGCTCGATTTATCAAGCAACATTAG

Coding sequence (CDS)

ATGATAATAAAAACTATAAAACAAATAGGTTGTTATATAAACCAACCCATTTTTCCTAATTTCTCTCCATCATCTTTCTTCTTCTTTGACGACGGCTCCGTCGACCGGACCTGGACCGGACCGCCGGAAGTCAAGTTCGTCGCGGAGTCTGTCCCACCTCACGACGACTTCATCGACGGAGTCGCCGTTCGTGACTTGGTGATTGATCAGAACTCTGGTCTCCGTGTTCGAATCTATTTACCAGAGGTGAAATGCAGCGGCGGAAAGAAACTGCTGGTTGTCATTCATTTTCACGGCGGTGGATTTTGTATTAGCGAAGCGGATTGGTATATGTATTATCATACTTACACAAACCTTGTGAGATCCGCCGAAGCAATTTGTGTCTCTGTTTACCTCCGTCGAGCGCCAGAGCATCGTCTTCCAGCGGCGATCGACGACGGCTTCTCCGCTCTCAAGTGGTTGCAATCGGTGGCTCTCGGTAACGAAACCGAGCCTTGGATTGTCGAAAACGCGGATTTCAATCGCGTTTTCCTAATCGGAGATAGCTCTGGCGGAAATCTCGTTCACTCTGTTGCAGCCCTGGCCGGAGAAATCGATCTCACTCCTTTGAAACTTGCCGGTGGAATTCCGATTCATCCTGGTTTTGTCAGAGCAAAACGAAGCAAATCAGAGATTGAGAATCCGCAATCGCCGTTCTTGACTTTGGATATGGTAGACAAATTTCTGAGCCTCTCATTACCGGTGGGGAGTACAAAGGATCATCCGATTACGTGTCCGATGGGAATCGACGCGCTGCCCCTTGAAAAGCTAAATCTGCCGCCATTTCTACTGTGCATTGCAGAGAAAGATCTGATAATCGACACCGAAATGGAGTATTACGAAGCAATGAAGGCGGCGAATAAAGAAGTGGAGATTTTAATGAGCAAAGGAATGGGACATAGCTTTTATCTGAACAAGATCGCCATGAAAATGGACCCTGAAACAGCTGCTGAATCGGACCGCCTCTTTGCCGGAATTGCTCGATTTATCAAGCAACATTAG

Protein sequence

MIIKTIKQIGCYINQPIFPNFSPSSFFFFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKCSGGKKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDGFSALKWLQSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAALAGEIDLTPLKLAGGIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMGIDALPLEKLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIAMKMDPETAAESDRLFAGIARFIKQH
Homology
BLAST of HG10004407 vs. NCBI nr
Match: XP_038886113.1 (probable carboxylesterase 15 [Benincasa hispida])

HSP 1 Score: 624.8 bits (1610), Expect = 4.5e-175
Identity = 300/318 (94.34%), Postives = 310/318 (97.48%), Query Frame = 0

Query: 29  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKCSGG 88
           FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKC  G
Sbjct: 18  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKCGRG 77

Query: 89  KKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDGF 148
           KKL V++HFHGGGFCISEADW+MYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDGF
Sbjct: 78  KKLPVIVHFHGGGFCISEADWFMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDGF 137

Query: 149 SALKWLQSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAALAGEIDLTPLKLAGG 208
           SALKWLQSVALGNE EPWIVENADFNRVFLIGDSSGGNLVHSVAALAGE DLTPLKLAGG
Sbjct: 138 SALKWLQSVALGNEIEPWIVENADFNRVFLIGDSSGGNLVHSVAALAGETDLTPLKLAGG 197

Query: 209 IPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMGIDALPLE 268
           IPIHPGFVRAKRSKSE+ENPQSPFLTLDMVDKFLSL+LPVGS+KDHPITCPMGIDA PLE
Sbjct: 198 IPIHPGFVRAKRSKSEMENPQSPFLTLDMVDKFLSLALPVGSSKDHPITCPMGIDAPPLE 257

Query: 269 KLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIAMKMDPET 328
           +LNLPPFLLCIA+KDLIIDTEMEYY+AMK ANKEVE+LMSKGMGHSFYLNKIAMKMDPET
Sbjct: 258 RLNLPPFLLCIADKDLIIDTEMEYYKAMKVANKEVELLMSKGMGHSFYLNKIAMKMDPET 317

Query: 329 AAESDRLFAGIARFIKQH 347
           AAESD LFAGIARFIKQH
Sbjct: 318 AAESDHLFAGIARFIKQH 335

BLAST of HG10004407 vs. NCBI nr
Match: XP_022997210.1 (probable carboxylesterase 15 [Cucurbita maxima])

HSP 1 Score: 608.6 bits (1568), Expect = 3.3e-170
Identity = 290/318 (91.19%), Postives = 308/318 (96.86%), Query Frame = 0

Query: 29  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKCSGG 88
           FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVID+ SGLRVRIYLPEVKCSGG
Sbjct: 18  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLRVRIYLPEVKCSGG 77

Query: 89  KKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDGF 148
           +KL VV+HFHGGGFCISEADW+MYYHTYT++V+SAEAICVSVYLRRAPEHRLPAA+DDGF
Sbjct: 78  EKLPVVVHFHGGGFCISEADWFMYYHTYTDVVKSAEAICVSVYLRRAPEHRLPAAVDDGF 137

Query: 149 SALKWLQSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAALAGEIDLTPLKLAGG 208
           SAL+WLQSVALG+E EPWI+ENADFNRVFLIGDSSGGNLVHSVAALAGE DL+P+KLAGG
Sbjct: 138 SALRWLQSVALGHEIEPWIIENADFNRVFLIGDSSGGNLVHSVAALAGEADLSPVKLAGG 197

Query: 209 IPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMGIDALPLE 268
           IPIHPGFVR++RSKSEIENPQSPFLTLDMVDKFLSL+LP GSTKDHPITCPMG +A PLE
Sbjct: 198 IPIHPGFVRSERSKSEIENPQSPFLTLDMVDKFLSLALPEGSTKDHPITCPMGANAQPLE 257

Query: 269 KLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIAMKMDPET 328
            LNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIA+KMDPET
Sbjct: 258 SLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIALKMDPET 317

Query: 329 AAESDRLFAGIARFIKQH 347
           AA SD LFAGIARFIKQH
Sbjct: 318 AAASDHLFAGIARFIKQH 335

BLAST of HG10004407 vs. NCBI nr
Match: KAG6598655.1 (putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 602.8 bits (1553), Expect = 1.8e-168
Identity = 289/318 (90.88%), Postives = 305/318 (95.91%), Query Frame = 0

Query: 29  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKCSGG 88
           FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVID+ SGL VRIYLPEVKCSGG
Sbjct: 18  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLCVRIYLPEVKCSGG 77

Query: 89  KKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDGF 148
           +KL VV+HFHGGGFCISEADW+MYYHTYT+LV+SAEAICVSVYLRRAPEHRLPAA+DDGF
Sbjct: 78  EKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSVYLRRAPEHRLPAAVDDGF 137

Query: 149 SALKWLQSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAALAGEIDLTPLKLAGG 208
           SAL+WLQSVALG+E EPWI ENADFNRVFLIGDSSGGNLVHSVAALAGE DL P+KLAGG
Sbjct: 138 SALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHSVAALAGEADLAPVKLAGG 197

Query: 209 IPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMGIDALPLE 268
           IPIHPGFVR++RSKSEIENPQSPFLTLDMVDKFLSL+LP GSTKDHPITCPMG +A PLE
Sbjct: 198 IPIHPGFVRSERSKSEIENPQSPFLTLDMVDKFLSLALPEGSTKDHPITCPMGANAPPLE 257

Query: 269 KLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIAMKMDPET 328
            LNLPPFLLCIAEKDLIIDTEMEYY+AMKAANKEVEILMSKGMGHSFYLNKIA+KMDPET
Sbjct: 258 TLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKGMGHSFYLNKIALKMDPET 317

Query: 329 AAESDRLFAGIARFIKQH 347
           AA SD LFAGIARFIKQH
Sbjct: 318 AAASDHLFAGIARFIKQH 335

BLAST of HG10004407 vs. NCBI nr
Match: XP_022961742.1 (probable carboxylesterase 15 [Cucurbita moschata])

HSP 1 Score: 601.3 bits (1549), Expect = 5.3e-168
Identity = 288/318 (90.57%), Postives = 304/318 (95.60%), Query Frame = 0

Query: 29  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKCSGG 88
           FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVID+ SGL VRIYLPEVKCSGG
Sbjct: 18  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLCVRIYLPEVKCSGG 77

Query: 89  KKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDGF 148
           +KL VV+HFHGGGFCISEADW+MYYHTYT++V+SAEAICVSVYLRRAPEHRLPAA+DDGF
Sbjct: 78  EKLPVVVHFHGGGFCISEADWFMYYHTYTDVVKSAEAICVSVYLRRAPEHRLPAAVDDGF 137

Query: 149 SALKWLQSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAALAGEIDLTPLKLAGG 208
           SAL+WLQSVALG+E EPWI ENADFNRVFLIGDSSGGNLVHSVAALAGE DL P+KLAGG
Sbjct: 138 SALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHSVAALAGEADLAPVKLAGG 197

Query: 209 IPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMGIDALPLE 268
           IPIHPGFVR++RSKSEIENPQSPFLTLDMVDKFLSL+LP GSTKDHPITCPMG +A PLE
Sbjct: 198 IPIHPGFVRSERSKSEIENPQSPFLTLDMVDKFLSLALPEGSTKDHPITCPMGANAPPLE 257

Query: 269 KLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIAMKMDPET 328
            LNLPPFLLCIAEKDLIIDTEMEYY AMKAANKEVEILMSKGMGHSFYLNKIA+KMDPET
Sbjct: 258 TLNLPPFLLCIAEKDLIIDTEMEYYRAMKAANKEVEILMSKGMGHSFYLNKIALKMDPET 317

Query: 329 AAESDRLFAGIARFIKQH 347
           AA SD LFAGIARFIKQH
Sbjct: 318 AAASDHLFAGIARFIKQH 335

BLAST of HG10004407 vs. NCBI nr
Match: KAA0064793.1 (putative carboxylesterase 15 [Cucumis melo var. makuwa])

HSP 1 Score: 600.9 bits (1548), Expect = 7.0e-168
Identity = 291/319 (91.22%), Postives = 303/319 (94.98%), Query Frame = 0

Query: 29  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKCSGG 88
           +DDGSVDRTWTGPPEVKFVAESVPPHD+FIDGVAVRDLVIDQNSGLRVRIYLPEVKC   
Sbjct: 18  YDDGSVDRTWTGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQNSGLRVRIYLPEVKCGAE 77

Query: 89  -KKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDG 148
            KKL VV+HFHGGGFCISEADW+MYYHTYTNL +SAEAICVSVYLRRAPEHRLPA I+DG
Sbjct: 78  VKKLPVVVHFHGGGFCISEADWFMYYHTYTNLAKSAEAICVSVYLRRAPEHRLPAQIEDG 137

Query: 149 FSALKWLQSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAALAGEIDLTPLKLAG 208
           FSALKWLQSVALGNE EPWIVE ADFNRVFLIGDSSGGNLVHSVAA AGE DL PLKLAG
Sbjct: 138 FSALKWLQSVALGNEIEPWIVEKADFNRVFLIGDSSGGNLVHSVAARAGETDLAPLKLAG 197

Query: 209 GIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMGIDALPL 268
           GIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFL+L+LPVGS+KDHPITCPMG  A PL
Sbjct: 198 GIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLNLALPVGSSKDHPITCPMGSAAPPL 257

Query: 269 EKLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIAMKMDPE 328
           E LNLPPFLLCIAEKDL+IDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIA+K+DPE
Sbjct: 258 ENLNLPPFLLCIAEKDLVIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIALKLDPE 317

Query: 329 TAAESDRLFAGIARFIKQH 347
           TAAESDRLFAGIARFIKQH
Sbjct: 318 TAAESDRLFAGIARFIKQH 336

BLAST of HG10004407 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.8e-44
Identity = 98/263 (37.26%), Postives = 147/263 (55.89%), Query Frame = 0

Query: 61  VAVRDLVIDQNSGLRVRIYLPEVKCSGGKKLLVVIHFHGGGFCISEADWYMYYHTYTNLV 120
           V  +D +  + + L +R+Y P +  S    L VV+ FHGGGFC     W  +++    L 
Sbjct: 47  VLFKDSIYHKPNNLHLRLYKP-ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLA 106

Query: 121 RSAEAICVSVYLRRAPEHRLPAAIDDGFSALKWLQSVALGNETEPWIVE--NADFNRVFL 180
            S  A+ VS   R APEHRLPAA +D  + L WL   A+ +    W  +  + DF+RVF+
Sbjct: 107 SSLNALVVSPDYRLAPEHRLPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFV 166

Query: 181 IGDSSGGNLVHSVAAL--AGEIDLTPLKLAGGIPIHPGFVRAKRSKSEIENPQSPFLTLD 240
           +GDSSGGN+ H +A    +G I+LTP+++ G + + P F   +R+ SE   P    L+LD
Sbjct: 167 VGDSSGGNIAHQLAVRFGSGSIELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLD 226

Query: 241 MVDKFLSLSLPVGSTKDHPITCPMGIDALPLEKLNLPPFLLCIAEKDLIIDTEMEY-YEA 300
           ++DKF  LSLP G+T+DH +  P G  +  LE ++L P L+ +   +L+ D   EY Y+ 
Sbjct: 227 LLDKFWRLSLPNGATRDHHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKL 286

Query: 301 MKAANKEVEILMSKGMGHSFYLN 319
            K   K V+ +  +   H FY N
Sbjct: 287 KKMGGKRVDYIEFENKEHGFYSN 307

BLAST of HG10004407 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.4e-38
Identity = 98/292 (33.56%), Postives = 155/292 (53.08%), Query Frame = 0

Query: 29  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKC-SG 88
           + DG V+R+   P         V P      GV   D+VID+ + +  R+Y+P     S 
Sbjct: 34  YKDGHVERSQLLP--------CVDPSLPLELGVTCSDVVIDKLTNVWARLYVPMTTTKSS 93

Query: 89  GKKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDG 148
             KL ++++FHGGGFC+  A W  Y+     L   +  + +SV  R APE+ LPAA +DG
Sbjct: 94  VSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLPAAYEDG 153

Query: 149 FSALKWLQSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAALAGEIDLTPLKLAG 208
            +A+ WL      N    W  +  DF R+FL GDS+GGN+   VAA     +   LK+ G
Sbjct: 154 VNAILWLNKARNDN---LW-AKQCDFGRIFLAGDSAGGNIAQQVAARLASPEDLALKIEG 213

Query: 209 GIPIHPGFVRAKRSKSE--IENPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMGIDAL 268
            I I P +   +R++SE  + N ++  LTL   D +  +SLP G+ ++HP   P+    +
Sbjct: 214 TILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANREHPYCKPV---KM 273

Query: 269 PLEKLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYL 318
            ++   +   L+C+AE DL++D+ ME  +  +   K V   + KG+GH+F++
Sbjct: 274 IIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNEDVIKRV---LHKGVGHAFHI 307

BLAST of HG10004407 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 7.0e-38
Identity = 100/296 (33.78%), Postives = 157/296 (53.04%), Query Frame = 0

Query: 29  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPE-VKCSG 88
           F+DG V+R    PP V  V+ ++ P           D+ +  ++    R+Y+P+    S 
Sbjct: 39  FNDGCVER----PPIVPIVSPTIHPSSK----ATAFDIKLSNDTW--TRVYIPDAAAASP 98

Query: 89  GKKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDG 148
              L ++++FHGGGFC+  A W  Y+   T+L   A  + VSV  R APEHRLPAA DDG
Sbjct: 99  SVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHRLPAAYDDG 158

Query: 149 FSALKWL--QSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAA--LAGEIDLTPL 208
            + + WL  Q ++ G     W+    + + VFL GDS+G N+ + VA   +A       L
Sbjct: 159 VNVVSWLVKQQISTGGGYPSWL-SKCNLSNVFLAGDSAGANIAYQVAVRIMASGKYANTL 218

Query: 209 KLAGGIPIHPGFVRAKRSKSEIE--NPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMG 268
            L G I IHP F    R+ SE +  + +S  LTL   D +  L+LP G+++DHP   P+ 
Sbjct: 219 HLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGASRDHPWCNPLM 278

Query: 269 IDALPLEKLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYL 318
             A       LP  ++ +AE D++ +  +E  + M++  K VE ++  G+GH+F++
Sbjct: 279 SSA----GAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGGVGHAFHI 319

BLAST of HG10004407 vs. ExPASy Swiss-Prot
Match: I4DST8 (Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA1 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.7e-36
Identity = 91/262 (34.73%), Postives = 143/262 (54.58%), Query Frame = 0

Query: 61  VAVRDLVIDQNSGLRVRIYLPEVKCSGGKKLLVVIHFHGGGFCISEADWYMYYHTYTNLV 120
           VA +D+VID  +G+ VR+YLP V     KKL V+++FHGGGF I       Y++  T L 
Sbjct: 114 VATKDVVIDPATGVSVRLYLPNVVDLPSKKLPVLVYFHGGGFVIENTGSPNYHNYLTLLA 173

Query: 121 RSAEAICVSVYLRRAPEHRLPAAIDDGFSALKWLQSVALGNETEPWIVENADFNRVFLIG 180
             A  + VS+  R APE+ LPA+ DD  +   W+ S + G   EPW+ ++ DF+++ L G
Sbjct: 174 AKAGVLIVSINYRLAPEYPLPASYDDCMAGFNWVVSHSAGPALEPWLAQHGDFSQILLSG 233

Query: 181 DSSGGNLVHSVA--ALAGEIDLTPLKLAGGIPIHPGFVRAKRSKSEIENPQSPFLTLDMV 240
           DS+GGN+ H VA  A AG I+       G   +HP F+ ++   +EI +P +    ++  
Sbjct: 234 DSAGGNVTHYVAMRADAGVIE-------GVAIVHPYFLGSEPVGNEINDPAN----IEFH 293

Query: 241 DKFLSLSLPVGSTKDHPITCPMGIDALPLEKLNLPPFLLCIAEKDLIIDTEMEYYEAMKA 300
           DK   L+ P     D P+  P+   A  L  L     ++ +A  D +++    YYEA+  
Sbjct: 294 DKLWRLAAPDTEGLDDPLINPVAPGAPSLAGLKCKRAVVFVAGNDFLVERGRMYYEALVK 353

Query: 301 A--NKEVEILMSKGMGHSFYLN 319
           +    E E++  +G+GH F+L+
Sbjct: 354 SGWRGEAELVQHEGVGHVFHLS 364

BLAST of HG10004407 vs. ExPASy Swiss-Prot
Match: I4DST9 (Tuliposide A-converting enzyme 2, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA2 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 3.0e-36
Identity = 91/262 (34.73%), Postives = 143/262 (54.58%), Query Frame = 0

Query: 61  VAVRDLVIDQNSGLRVRIYLPEVKCSGGKKLLVVIHFHGGGFCISEADWYMYYHTYTNLV 120
           VA +D+VID  +G+ VR+YLP V     KKL V+++FHGGGF I       Y++  T L 
Sbjct: 111 VATKDVVIDPATGVSVRLYLPNVVDLPSKKLPVLVYFHGGGFVIENTGSPNYHNYLTLLA 170

Query: 121 RSAEAICVSVYLRRAPEHRLPAAIDDGFSALKWLQSVALGNETEPWIVENADFNRVFLIG 180
             A  + VS+  R APE+ LPA+ DD  +   W+ S + G   EPW+ ++ DF+++ L G
Sbjct: 171 AKAGVLIVSINYRLAPEYPLPASYDDCMAGFNWVVSHSAGPALEPWLAQHGDFSQILLSG 230

Query: 181 DSSGGNLVHSVA--ALAGEIDLTPLKLAGGIPIHPGFVRAKRSKSEIENPQSPFLTLDMV 240
           DS+GGN+ H VA  A AG I+       G   +HP F+ ++   +EI +P +    ++  
Sbjct: 231 DSAGGNVTHYVAMRADAGVIE-------GVAIVHPYFLGSEPVGNEINDPAN----IEFH 290

Query: 241 DKFLSLSLPVGSTKDHPITCPMGIDALPLEKLNLPPFLLCIAEKDLIIDTEMEYYEAMKA 300
           DK   L+ P     D P+  P+   A  L  L     ++ +A  D +++    YYEA+  
Sbjct: 291 DKLWRLAAPDTEGLDDPLINPVAPGAPILAGLKCKRAVVFVAGNDFLVERGRMYYEALVK 350

Query: 301 A--NKEVEILMSKGMGHSFYLN 319
           +    E E++  +G+GH F+L+
Sbjct: 351 SGWGGEAELVQHEGVGHVFHLS 361

BLAST of HG10004407 vs. ExPASy TrEMBL
Match: A0A6J1K8Y5 (probable carboxylesterase 15 OS=Cucurbita maxima OX=3661 GN=LOC111492182 PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.6e-170
Identity = 290/318 (91.19%), Postives = 308/318 (96.86%), Query Frame = 0

Query: 29  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKCSGG 88
           FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVID+ SGLRVRIYLPEVKCSGG
Sbjct: 18  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLRVRIYLPEVKCSGG 77

Query: 89  KKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDGF 148
           +KL VV+HFHGGGFCISEADW+MYYHTYT++V+SAEAICVSVYLRRAPEHRLPAA+DDGF
Sbjct: 78  EKLPVVVHFHGGGFCISEADWFMYYHTYTDVVKSAEAICVSVYLRRAPEHRLPAAVDDGF 137

Query: 149 SALKWLQSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAALAGEIDLTPLKLAGG 208
           SAL+WLQSVALG+E EPWI+ENADFNRVFLIGDSSGGNLVHSVAALAGE DL+P+KLAGG
Sbjct: 138 SALRWLQSVALGHEIEPWIIENADFNRVFLIGDSSGGNLVHSVAALAGEADLSPVKLAGG 197

Query: 209 IPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMGIDALPLE 268
           IPIHPGFVR++RSKSEIENPQSPFLTLDMVDKFLSL+LP GSTKDHPITCPMG +A PLE
Sbjct: 198 IPIHPGFVRSERSKSEIENPQSPFLTLDMVDKFLSLALPEGSTKDHPITCPMGANAQPLE 257

Query: 269 KLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIAMKMDPET 328
            LNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIA+KMDPET
Sbjct: 258 SLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIALKMDPET 317

Query: 329 AAESDRLFAGIARFIKQH 347
           AA SD LFAGIARFIKQH
Sbjct: 318 AAASDHLFAGIARFIKQH 335

BLAST of HG10004407 vs. ExPASy TrEMBL
Match: A0A6J1HAY3 (probable carboxylesterase 15 OS=Cucurbita moschata OX=3662 GN=LOC111462421 PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.6e-168
Identity = 288/318 (90.57%), Postives = 304/318 (95.60%), Query Frame = 0

Query: 29  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKCSGG 88
           FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVID+ SGL VRIYLPEVKCSGG
Sbjct: 18  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLCVRIYLPEVKCSGG 77

Query: 89  KKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDGF 148
           +KL VV+HFHGGGFCISEADW+MYYHTYT++V+SAEAICVSVYLRRAPEHRLPAA+DDGF
Sbjct: 78  EKLPVVVHFHGGGFCISEADWFMYYHTYTDVVKSAEAICVSVYLRRAPEHRLPAAVDDGF 137

Query: 149 SALKWLQSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAALAGEIDLTPLKLAGG 208
           SAL+WLQSVALG+E EPWI ENADFNRVFLIGDSSGGNLVHSVAALAGE DL P+KLAGG
Sbjct: 138 SALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHSVAALAGEADLAPVKLAGG 197

Query: 209 IPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMGIDALPLE 268
           IPIHPGFVR++RSKSEIENPQSPFLTLDMVDKFLSL+LP GSTKDHPITCPMG +A PLE
Sbjct: 198 IPIHPGFVRSERSKSEIENPQSPFLTLDMVDKFLSLALPEGSTKDHPITCPMGANAPPLE 257

Query: 269 KLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIAMKMDPET 328
            LNLPPFLLCIAEKDLIIDTEMEYY AMKAANKEVEILMSKGMGHSFYLNKIA+KMDPET
Sbjct: 258 TLNLPPFLLCIAEKDLIIDTEMEYYRAMKAANKEVEILMSKGMGHSFYLNKIALKMDPET 317

Query: 329 AAESDRLFAGIARFIKQH 347
           AA SD LFAGIARFIKQH
Sbjct: 318 AAASDHLFAGIARFIKQH 335

BLAST of HG10004407 vs. ExPASy TrEMBL
Match: A0A5A7V9G7 (Putative carboxylesterase 15 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001000 PE=4 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 3.4e-168
Identity = 291/319 (91.22%), Postives = 303/319 (94.98%), Query Frame = 0

Query: 29  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKCSGG 88
           +DDGSVDRTWTGPPEVKFVAESVPPHD+FIDGVAVRDLVIDQNSGLRVRIYLPEVKC   
Sbjct: 18  YDDGSVDRTWTGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQNSGLRVRIYLPEVKCGAE 77

Query: 89  -KKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDG 148
            KKL VV+HFHGGGFCISEADW+MYYHTYTNL +SAEAICVSVYLRRAPEHRLPA I+DG
Sbjct: 78  VKKLPVVVHFHGGGFCISEADWFMYYHTYTNLAKSAEAICVSVYLRRAPEHRLPAQIEDG 137

Query: 149 FSALKWLQSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAALAGEIDLTPLKLAG 208
           FSALKWLQSVALGNE EPWIVE ADFNRVFLIGDSSGGNLVHSVAA AGE DL PLKLAG
Sbjct: 138 FSALKWLQSVALGNEIEPWIVEKADFNRVFLIGDSSGGNLVHSVAARAGETDLAPLKLAG 197

Query: 209 GIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMGIDALPL 268
           GIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFL+L+LPVGS+KDHPITCPMG  A PL
Sbjct: 198 GIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLNLALPVGSSKDHPITCPMGSAAPPL 257

Query: 269 EKLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIAMKMDPE 328
           E LNLPPFLLCIAEKDL+IDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIA+K+DPE
Sbjct: 258 ENLNLPPFLLCIAEKDLVIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIALKLDPE 317

Query: 329 TAAESDRLFAGIARFIKQH 347
           TAAESDRLFAGIARFIKQH
Sbjct: 318 TAAESDRLFAGIARFIKQH 336

BLAST of HG10004407 vs. ExPASy TrEMBL
Match: A0A1S3BCL7 (probable carboxylesterase 15 OS=Cucumis melo OX=3656 GN=LOC103488436 PE=4 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 5.7e-168
Identity = 291/319 (91.22%), Postives = 303/319 (94.98%), Query Frame = 0

Query: 29  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKCSGG 88
           +DDGSVDRTWTGPPEVKFVAESVPPHD+FIDGVAVRDLVIDQNSGLRVRIYLPEVKC G 
Sbjct: 18  YDDGSVDRTWTGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQNSGLRVRIYLPEVKCGGE 77

Query: 89  -KKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDG 148
            KKL VV+HFHGGGFCISEADW+MYYHTYTNL +SAEAICVSVYLRRAPEHRLPA I+DG
Sbjct: 78  VKKLPVVVHFHGGGFCISEADWFMYYHTYTNLAKSAEAICVSVYLRRAPEHRLPAQIEDG 137

Query: 149 FSALKWLQSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAALAGEIDLTPLKLAG 208
           FSALKWLQSVALGNE EPWIVE ADFNRVFLIGDSSGGNLVHSVAA AGE DL PLKLAG
Sbjct: 138 FSALKWLQSVALGNEIEPWIVEKADFNRVFLIGDSSGGNLVHSVAARAGETDLAPLKLAG 197

Query: 209 GIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMGIDALPL 268
           GIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFL+L+LPVGS+KDHPITCPMG  A PL
Sbjct: 198 GIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLNLALPVGSSKDHPITCPMGSAAPPL 257

Query: 269 EKLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIAMKMDPE 328
           E LNLPPFLLCIAEKDL+IDTEMEYYEAMKAANKEVEILMSK MGHSFYLNKIA+K+DPE
Sbjct: 258 ENLNLPPFLLCIAEKDLVIDTEMEYYEAMKAANKEVEILMSKEMGHSFYLNKIALKLDPE 317

Query: 329 TAAESDRLFAGIARFIKQH 347
           TAAESDRLFAGIARFIKQH
Sbjct: 318 TAAESDRLFAGIARFIKQH 336

BLAST of HG10004407 vs. ExPASy TrEMBL
Match: A0A0A0KDX8 (Catalytic OS=Cucumis sativus OX=3659 GN=Csa_6G401340 PE=4 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 9.2e-166
Identity = 285/319 (89.34%), Postives = 303/319 (94.98%), Query Frame = 0

Query: 29  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKCSGG 88
           +DDGSVDRTW GPPEVKFVAESVPPHD+FIDGVAVRDLVIDQNSGLRVRIYLPEVKC G 
Sbjct: 18  YDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQNSGLRVRIYLPEVKCGGE 77

Query: 89  -KKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDG 148
            KKL VV+HFHGGGFCISEADW+MYYHTYTN V+SAEAICVSVYLRRAPEHRLPAAI+DG
Sbjct: 78  VKKLPVVLHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSVYLRRAPEHRLPAAIEDG 137

Query: 149 FSALKWLQSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAALAGEIDLTPLKLAG 208
            S LKWLQSVALG+E EPWIVENADFNRVFLIGDS+GGNLVHSVAALAGE DL PLKLAG
Sbjct: 138 LSGLKWLQSVALGDEIEPWIVENADFNRVFLIGDSAGGNLVHSVAALAGETDLAPLKLAG 197

Query: 209 GIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMGIDALPL 268
           GIPIHPGFVRAKRSKSE+ENPQSPFL LDMVD FL+L+LPVGS+KD+PITCPMG  A PL
Sbjct: 198 GIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGSSKDNPITCPMGRAAPPL 257

Query: 269 EKLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYLNKIAMKMDPE 328
           EKLNLPPFLLC+AEKDL+IDT+MEYYEAMKAANKEVEILMSKGMGHSFYLNKIA+K+DPE
Sbjct: 258 EKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKGMGHSFYLNKIALKLDPE 317

Query: 329 TAAESDRLFAGIARFIKQH 347
           TAAESDRLFAGIARFIKQH
Sbjct: 318 TAAESDRLFAGIARFIKQH 336

BLAST of HG10004407 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.7e-45
Identity = 98/263 (37.26%), Postives = 147/263 (55.89%), Query Frame = 0

Query: 61  VAVRDLVIDQNSGLRVRIYLPEVKCSGGKKLLVVIHFHGGGFCISEADWYMYYHTYTNLV 120
           V  +D +  + + L +R+Y P +  S    L VV+ FHGGGFC     W  +++    L 
Sbjct: 47  VLFKDSIYHKPNNLHLRLYKP-ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLA 106

Query: 121 RSAEAICVSVYLRRAPEHRLPAAIDDGFSALKWLQSVALGNETEPWIVE--NADFNRVFL 180
            S  A+ VS   R APEHRLPAA +D  + L WL   A+ +    W  +  + DF+RVF+
Sbjct: 107 SSLNALVVSPDYRLAPEHRLPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFV 166

Query: 181 IGDSSGGNLVHSVAAL--AGEIDLTPLKLAGGIPIHPGFVRAKRSKSEIENPQSPFLTLD 240
           +GDSSGGN+ H +A    +G I+LTP+++ G + + P F   +R+ SE   P    L+LD
Sbjct: 167 VGDSSGGNIAHQLAVRFGSGSIELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLD 226

Query: 241 MVDKFLSLSLPVGSTKDHPITCPMGIDALPLEKLNLPPFLLCIAEKDLIIDTEMEY-YEA 300
           ++DKF  LSLP G+T+DH +  P G  +  LE ++L P L+ +   +L+ D   EY Y+ 
Sbjct: 227 LLDKFWRLSLPNGATRDHHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKL 286

Query: 301 MKAANKEVEILMSKGMGHSFYLN 319
            K   K V+ +  +   H FY N
Sbjct: 287 KKMGGKRVDYIEFENKEHGFYSN 307

BLAST of HG10004407 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 2.7e-45
Identity = 98/263 (37.26%), Postives = 147/263 (55.89%), Query Frame = 0

Query: 61  VAVRDLVIDQNSGLRVRIYLPEVKCSGGKKLLVVIHFHGGGFCISEADWYMYYHTYTNLV 120
           V  +D +  + + L +R+Y P +  S    L VV+ FHGGGFC     W  +++    L 
Sbjct: 47  VLFKDSIYHKPNNLHLRLYKP-ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLA 106

Query: 121 RSAEAICVSVYLRRAPEHRLPAAIDDGFSALKWLQSVALGNETEPWIVE--NADFNRVFL 180
            S  A+ VS   R APEHRLPAA +D  + L WL   A+ +    W  +  + DF+RVF+
Sbjct: 107 SSLNALVVSPDYRLAPEHRLPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFV 166

Query: 181 IGDSSGGNLVHSVAAL--AGEIDLTPLKLAGGIPIHPGFVRAKRSKSEIENPQSPFLTLD 240
           +GDSSGGN+ H +A    +G I+LTP+++ G + + P F   +R+ SE   P    L+LD
Sbjct: 167 VGDSSGGNIAHQLAVRFGSGSIELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLD 226

Query: 241 MVDKFLSLSLPVGSTKDHPITCPMGIDALPLEKLNLPPFLLCIAEKDLIIDTEMEY-YEA 300
           ++DKF  LSLP G+T+DH +  P G  +  LE ++L P L+ +   +L+ D   EY Y+ 
Sbjct: 227 LLDKFWRLSLPNGATRDHHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKL 286

Query: 301 MKAANKEVEILMSKGMGHSFYLN 319
            K   K V+ +  +   H FY N
Sbjct: 287 KKMGGKRVDYIEFENKEHGFYSN 307

BLAST of HG10004407 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 161.0 bits (406), Expect = 1.7e-39
Identity = 98/292 (33.56%), Postives = 155/292 (53.08%), Query Frame = 0

Query: 29  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKC-SG 88
           + DG V+R+   P         V P      GV   D+VID+ + +  R+Y+P     S 
Sbjct: 34  YKDGHVERSQLLP--------CVDPSLPLELGVTCSDVVIDKLTNVWARLYVPMTTTKSS 93

Query: 89  GKKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDG 148
             KL ++++FHGGGFC+  A W  Y+     L   +  + +SV  R APE+ LPAA +DG
Sbjct: 94  VSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPLPAAYEDG 153

Query: 149 FSALKWLQSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAALAGEIDLTPLKLAG 208
            +A+ WL      N    W  +  DF R+FL GDS+GGN+   VAA     +   LK+ G
Sbjct: 154 VNAILWLNKARNDN---LW-AKQCDFGRIFLAGDSAGGNIAQQVAARLASPEDLALKIEG 213

Query: 209 GIPIHPGFVRAKRSKSE--IENPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMGIDAL 268
            I I P +   +R++SE  + N ++  LTL   D +  +SLP G+ ++HP   P+    +
Sbjct: 214 TILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANREHPYCKPV---KM 273

Query: 269 PLEKLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYL 318
            ++   +   L+C+AE DL++D+ ME  +  +   K V   + KG+GH+F++
Sbjct: 274 IIKSSTVTRTLVCVAEMDLLMDSNMEMCDGNEDVIKRV---LHKGVGHAFHI 307

BLAST of HG10004407 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 159.5 bits (402), Expect = 5.0e-39
Identity = 100/296 (33.78%), Postives = 157/296 (53.04%), Query Frame = 0

Query: 29  FDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPE-VKCSG 88
           F+DG V+R    PP V  V+ ++ P           D+ +  ++    R+Y+P+    S 
Sbjct: 39  FNDGCVER----PPIVPIVSPTIHPSSK----ATAFDIKLSNDTW--TRVYIPDAAAASP 98

Query: 89  GKKLLVVIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDG 148
              L ++++FHGGGFC+  A W  Y+   T+L   A  + VSV  R APEHRLPAA DDG
Sbjct: 99  SVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHRLPAAYDDG 158

Query: 149 FSALKWL--QSVALGNETEPWIVENADFNRVFLIGDSSGGNLVHSVAA--LAGEIDLTPL 208
            + + WL  Q ++ G     W+    + + VFL GDS+G N+ + VA   +A       L
Sbjct: 159 VNVVSWLVKQQISTGGGYPSWL-SKCNLSNVFLAGDSAGANIAYQVAVRIMASGKYANTL 218

Query: 209 KLAGGIPIHPGFVRAKRSKSEIE--NPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMG 268
            L G I IHP F    R+ SE +  + +S  LTL   D +  L+LP G+++DHP   P+ 
Sbjct: 219 HLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGASRDHPWCNPLM 278

Query: 269 IDALPLEKLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYL 318
             A       LP  ++ +AE D++ +  +E  + M++  K VE ++  G+GH+F++
Sbjct: 279 SSA----GAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGGVGHAFHI 319

BLAST of HG10004407 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 153.7 bits (387), Expect = 2.7e-37
Identity = 98/285 (34.39%), Postives = 150/285 (52.63%), Query Frame = 0

Query: 45  KFVAESVPPHDDFIDGVAVRDLVIDQNSGLRVRIYLPEVKCSG-----------GKKLLV 104
           +F+   VP + + ++GV   D++ID+ + L  R+Y P    +            G+ + V
Sbjct: 47  EFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGTSPSITDLQNPVDGEIVPV 106

Query: 105 VIHFHGGGFCISEADWYMYYHTYTNLVRSAEAICVSVYLRRAPEHRLPAAIDDGFSALKW 164
           ++ FHGG F  S A+  +Y      LV    A+ VSV  RRAPE+R P A DDG++ LKW
Sbjct: 107 IVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKW 166

Query: 165 LQSVALGNETEPWIVENADFN-RVFLIGDSSGGNLVHSVAALAGEIDLTPLKLAGGIPIH 224
           + S         W+    D   R+FL GDSSGGN+VH+VA  A E   + + + G I ++
Sbjct: 167 VNS-------SSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLN 226

Query: 225 PGFVRAKRSKSEIENPQSPFLTLDMVDKFLSLSLPVGSTKDHPITCPMGIDALPLEKLNL 284
           P F   +R++SE       F+T+   D +    LP G  ++HP   P G  +  LE L+ 
Sbjct: 227 PMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGLSF 286

Query: 285 PPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKGMGHSFYL 318
           P  L+ +A  DLI D +++Y E +K A +EV++L  +     FYL
Sbjct: 287 PKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYL 321

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038886113.14.5e-17594.34probable carboxylesterase 15 [Benincasa hispida][more]
XP_022997210.13.3e-17091.19probable carboxylesterase 15 [Cucurbita maxima][more]
KAG6598655.11.8e-16890.88putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022961742.15.3e-16890.57probable carboxylesterase 15 [Cucurbita moschata][more]
KAA0064793.17.0e-16891.22putative carboxylesterase 15 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9FG133.8e-4437.26Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Q9SX252.4e-3833.56Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1[more]
Q9LFR77.0e-3833.78Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1[more]
I4DST81.7e-3634.73Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN... [more]
I4DST93.0e-3634.73Tuliposide A-converting enzyme 2, chloroplastic OS=Tulipa gesneriana OX=13306 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1K8Y51.6e-17091.19probable carboxylesterase 15 OS=Cucurbita maxima OX=3661 GN=LOC111492182 PE=4 SV... [more]
A0A6J1HAY32.6e-16890.57probable carboxylesterase 15 OS=Cucurbita moschata OX=3662 GN=LOC111462421 PE=4 ... [more]
A0A5A7V9G73.4e-16891.22Putative carboxylesterase 15 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3BCL75.7e-16891.22probable carboxylesterase 15 OS=Cucumis melo OX=3656 GN=LOC103488436 PE=4 SV=1[more]
A0A0A0KDX89.2e-16689.34Catalytic OS=Cucumis sativus OX=3659 GN=Csa_6G401340 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G06570.12.7e-4537.26alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.22.7e-4537.26alpha/beta-Hydrolases superfamily protein [more]
AT1G68620.11.7e-3933.56alpha/beta-Hydrolases superfamily protein [more]
AT5G16080.15.0e-3933.78carboxyesterase 17 [more]
AT5G27320.12.7e-3734.39alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 94..316
e-value: 1.9E-48
score: 165.1
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 22..345
e-value: 4.0E-71
score: 242.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 35..346
NoneNo IPR availablePANTHERPTHR23024:SF135CELL DEATH ASSOCIATED PROTEINcoord: 43..343
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 43..343

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10004407.1HG10004407.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity