HG10004361 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10004361 vs. NCBI nr
Match: XP_023546079.1 (COBW domain-containing protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 679.5 bits (1752), Expect = 1.8e-191
Identity = 347/395 (87.85%), Postives = 361/395 (91.39%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIG TITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYA 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 LAIISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLESS+KLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSVKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYE 240
           SIITVVDAKNLHFQLNEHRSSSSFPEAF+QIA+ADTIILNKVDLV+SERGDGALE LE+E
Sbjct: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFYQIAFADTIILNKVDLVTSERGDGALEDLEFE 240

Query: 241 IRNINSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTL 300
           IRNINSLAKI+HSVRCQV+LSLILNCN+YNAANTAHLE LLKES SLST+DLHDTGVRTL
Sbjct: 241 IRNINSLAKIIHSVRCQVDLSLILNCNAYNAANTAHLEELLKESRSLSTKDLHDTGVRTL 300

Query: 301 CISDHDKVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEIVPT 360
           CIS HDKVDLDKV SWLEEILW+KKGGMDVYRCKGVLSIKNSD+LHTLQAVRE+YEIVPT
Sbjct: 301 CISYHDKVDLDKVRSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVREIYEIVPT 360

Query: 361 RQWNNGESQMNKIVFIGRNLNEDVLSETFRACAIS 396
           RQWNNGESQMNKIVFIGRNL+EDVLSETFRACA+S
Sbjct: 361 RQWNNGESQMNKIVFIGRNLSEDVLSETFRACAVS 367

BLAST of HG10004361 vs. NCBI nr
Match: XP_022962150.1 (COBW domain-containing protein 1 [Cucurbita moschata])

HSP 1 Score: 679.1 bits (1751), Expect = 2.3e-191
Identity = 347/395 (87.85%), Postives = 361/395 (91.39%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIG TITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYA 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 LAIISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLESS+KLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSVKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYE 240
           SIITVVDAKNLHFQLNEHRSSSSFPEAF+QIA+ADTIILNKVDLV+SERGDGALE LE+E
Sbjct: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFYQIAFADTIILNKVDLVTSERGDGALEDLEFE 240

Query: 241 IRNINSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTL 300
           IRNINSLAKI+HSVRCQV+LSLILNCN+YNAANTAHLE LLKES SLST+DLHDTGVRTL
Sbjct: 241 IRNINSLAKIIHSVRCQVDLSLILNCNAYNAANTAHLEELLKESRSLSTKDLHDTGVRTL 300

Query: 301 CISDHDKVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEIVPT 360
           CIS HDKVDLDKV SWLEEILW++KGGMDVYRCKGVLSIKNSD+LHTLQAVRELYEIVPT
Sbjct: 301 CISYHDKVDLDKVRSWLEEILWDRKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIVPT 360

Query: 361 RQWNNGESQMNKIVFIGRNLNEDVLSETFRACAIS 396
           RQWNNGESQMNKIVFIGRNL+EDVLSETFRACA+S
Sbjct: 361 RQWNNGESQMNKIVFIGRNLSEDVLSETFRACAVS 367

BLAST of HG10004361 vs. NCBI nr
Match: KAG6598632.1 (COBW domain-containing protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 677.2 bits (1746), Expect = 8.7e-191
Identity = 346/395 (87.59%), Postives = 360/395 (91.14%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIG TITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYA 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 LAIISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLESS+KLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSVKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYE 240
           SIITVVDAKNLHFQLNEHRSSSSFPEAF+QIA+ADTIILNKVDLV+SERGDGALE LE+E
Sbjct: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFYQIAFADTIILNKVDLVTSERGDGALEDLEFE 240

Query: 241 IRNINSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTL 300
           I NINSLAKI+HSVRCQV+LSLILNCN+YNAANTAHLE LLKES SLST+DLHDTGVRTL
Sbjct: 241 IHNINSLAKIIHSVRCQVDLSLILNCNAYNAANTAHLEELLKESRSLSTKDLHDTGVRTL 300

Query: 301 CISDHDKVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEIVPT 360
           CIS HDKVDLDKV SWLEEILW++KGGMDVYRCKGVLSIKNSD+LHTLQAVRELYEIVPT
Sbjct: 301 CISYHDKVDLDKVRSWLEEILWDRKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIVPT 360

Query: 361 RQWNNGESQMNKIVFIGRNLNEDVLSETFRACAIS 396
           RQWNNGESQMNKIVFIGRNL+EDVLSETFRACA+S
Sbjct: 361 RQWNNGESQMNKIVFIGRNLSEDVLSETFRACAVS 367

BLAST of HG10004361 vs. NCBI nr
Match: XP_038886766.1 (COBW domain-containing protein 1 isoform X1 [Benincasa hispida] >XP_038886768.1 COBW domain-containing protein 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 674.1 bits (1738), Expect = 7.4e-190
Identity = 348/395 (88.10%), Postives = 356/395 (90.13%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDE+DPPLAVEIG TITA  R+EDD VSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEKDPPLAVEIGETITAVGRYEDDSVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYA 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 LAIISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLESSIKLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYE 240
           SIITVVDAKNL FQLNEHRSSSSFPEAFHQIA+ADTIILNKVDLVSSE GDGALE LEYE
Sbjct: 181 SIITVVDAKNLRFQLNEHRSSSSFPEAFHQIAFADTIILNKVDLVSSECGDGALEDLEYE 240

Query: 241 IRNINSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTL 300
           IRNINSLAKIVHSVRCQVNLSLILNCNSYN+ NTAHLEALLKE+ SLSTRDLHDTGVRTL
Sbjct: 241 IRNINSLAKIVHSVRCQVNLSLILNCNSYNSDNTAHLEALLKETRSLSTRDLHDTGVRTL 300

Query: 301 CISDHDKVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEIVPT 360
           CIS+H KVDLDKVH WLEEILWEKKGGMDVYRCKGVLSIKNSD+LHTLQAVRELYEIVPT
Sbjct: 301 CISNHGKVDLDKVHCWLEEILWEKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIVPT 360

Query: 361 RQWNNGESQMNKIVFIGRNLNEDVLSETFRACAIS 396
           RQWNNGESQMNKIVFIGRNLNEDVLSETFRACA+S
Sbjct: 361 RQWNNGESQMNKIVFIGRNLNEDVLSETFRACAVS 367

BLAST of HG10004361 vs. NCBI nr
Match: XP_022997190.1 (COBW domain-containing protein 1 [Cucurbita maxima])

HSP 1 Score: 673.7 bits (1737), Expect = 9.6e-190
Identity = 344/395 (87.09%), Postives = 360/395 (91.14%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIG TITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYA 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 LAIISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLESS+KLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSVKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYE 240
           SIITVVDA+NLHFQLNEHRSSSSFPEAF+QIA+ADTIILNKVDLV+SERGDGALE LE+E
Sbjct: 181 SIITVVDARNLHFQLNEHRSSSSFPEAFYQIAFADTIILNKVDLVTSERGDGALEDLEFE 240

Query: 241 IRNINSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTL 300
           IRNINSLAKI+HS+RCQV+LSLILNCN+YNAANTAHLE LLKES SLST+DLHDTGVRTL
Sbjct: 241 IRNINSLAKIIHSIRCQVDLSLILNCNAYNAANTAHLEELLKESRSLSTKDLHDTGVRTL 300

Query: 301 CISDHDKVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEIVPT 360
           CIS HDKVDLDKV SWLEEILW+KKGGMDVYRCKGVLSIKNSD+LHTLQAVRELYEIVPT
Sbjct: 301 CISYHDKVDLDKVRSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIVPT 360

Query: 361 RQWNNGESQMNKIVFIGRNLNEDVLSETFRACAIS 396
           RQWNN ESQMNKIVFIGRNL+E+VLSETFRACA+S
Sbjct: 361 RQWNNRESQMNKIVFIGRNLSENVLSETFRACAVS 367

BLAST of HG10004361 vs. ExPASy Swiss-Prot
Match: Q99MB4 (COBW domain-containing protein 1 OS=Rattus norvegicus OX=10116 GN=Cbwd1 PE=2 SV=2)

HSP 1 Score: 246.1 bits (627), Expect = 6.5e-64
Identity = 151/395 (38.23%), Postives = 228/395 (57.72%), Query Frame = 0

Query: 2   EDEEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIAV 61
           E  ED P  V I +     E + D  + + VT++TGYLGAGK+TL+NYIL  QH ++IAV
Sbjct: 13  EYAEDCPELVPIETKHQEKEENLDFIIKIPVTIVTGYLGAGKTTLLNYILTEQHNRKIAV 72

Query: 62  ILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYAL 121
           ILNEFGE   VE+++     GG L EEW+EL NGC+CC+VK + ++A+E L+Q+K ++  
Sbjct: 73  ILNEFGEGSAVEKSLA-VSQGGELYEEWLELRNGCLCCSVKDNGLKAIENLMQKKGKF-- 132

Query: 122 AIISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLDS 181
                                     D+ILLETTGLA+P  +AS+ W+D +L S I LD 
Sbjct: 133 --------------------------DYILLETTGLADPGAVASMFWVDAELGSDIYLDG 192

Query: 182 IITVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYEI 241
           IITVVD+K     L E +      EA  Q+A AD I++NK DLVS E     L  L   I
Sbjct: 193 IITVVDSKYGLKHLTEEKPDGLVNEATRQVALADMILINKTDLVSEEE----LNKLRTTI 252

Query: 242 RNINSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTLC 301
           R+IN L K++ + R + +LS IL+ ++Y+  +   L+  L+    +ST    D  + T+ 
Sbjct: 253 RSINGLGKVLETQRSRTHLSNILDLHAYDTLSGISLQKKLQH---VSTAPHLDQSIVTVT 312

Query: 302 ISDHDKVDLDKVHSWLEEILWEK----KGG--MDVYRCKGVLSIKNSDKLHTLQAVRELY 361
                  + + ++ +++ +LWEK    K G  M+V R KG++SIK+  +   +Q + ELY
Sbjct: 313 FDVPGSAEEESLNVFIQNLLWEKNVKNKDGRCMEVIRLKGLVSIKDKPQQMIVQGIHELY 371

Query: 362 EIVPTR-QWNNGESQMNKIVFIGRNLNEDVLSETF 390
           E+  +R  W +   +  ++VFIG+NL++D+L + F
Sbjct: 373 ELEESRVNWKDDAERACQLVFIGKNLDKDILQQLF 371

BLAST of HG10004361 vs. ExPASy Swiss-Prot
Match: Q9BRT8 (COBW domain-containing protein 1 OS=Homo sapiens OX=9606 GN=CBWD1 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.5e-63
Identity = 148/395 (37.47%), Postives = 226/395 (57.22%), Query Frame = 0

Query: 4   EEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIAVIL 63
           EED P  V I +T +  E     G  + VT+ITGYLGAGK+TL+NYIL  QH KR+AVIL
Sbjct: 16  EEDCPELVPIETTQSEEEEKSGLGAKIPVTIITGYLGAGKTTLLNYILTEQHSKRVAVIL 75

Query: 64  NEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYALAI 123
           NEFGE   +E+++     GG L EEW+EL NGC+CC+VK S ++A+E L+Q+K ++    
Sbjct: 76  NEFGEGSALEKSLA-VSQGGELYEEWLELRNGCLCCSVKDSGLRAIENLMQKKGKF---- 135

Query: 124 ISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLDSII 183
                                   D+ILLETTGLA+P  +AS+ W+D +L S I LD II
Sbjct: 136 ------------------------DYILLETTGLADPGAVASMFWVDAELGSDIYLDGII 195

Query: 184 TVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYEIRN 243
           T+VD+K     L E +      EA  Q+A AD I++NK DLV  E     ++ L   IR+
Sbjct: 196 TIVDSKYGLKHLTEEKPDGLINEATRQVALADAILINKTDLVPEE----DVKKLRTTIRS 255

Query: 244 INSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTLCIS 303
           IN L +I+ + R +V+LS +L+ +++++ +   L+  L+      T+   D  + T+   
Sbjct: 256 INGLGQILETQRSRVDLSNVLDLHAFDSLSGISLQKKLQHVP--GTQPHLDQSIVTITFE 315

Query: 304 DHDKVDLDKVHSWLEEILWEK------KGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEI 363
                  + ++ +++ +LWEK         M+V R KG++SIK+  +   +Q V ELY++
Sbjct: 316 VPGNAKEEHLNMFIQNLLWEKNVRNKDNHCMEVIRLKGLVSIKDKSQQVIVQGVHELYDL 375

Query: 364 VPTR-QWNNGESQMNKIVFIGRNLNEDVLSETFRA 392
             T   W +   + N++V +GRNL++D+L + F A
Sbjct: 376 EETPVSWKDDTERTNRLVLLGRNLDKDILKQLFIA 375

BLAST of HG10004361 vs. ExPASy Swiss-Prot
Match: Q8VEH6 (COBW domain-containing protein 1 OS=Mus musculus OX=10090 GN=Cbwd1 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.2e-62
Identity = 154/395 (38.99%), Postives = 226/395 (57.22%), Query Frame = 0

Query: 2   EDEEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIAV 61
           E  ED P  V I  T    E + D    + VT++TGYLGAGK+TL+NYIL  QH ++IAV
Sbjct: 13  EYAEDCPELVPI-ETKNQEEENLDFITKIPVTIVTGYLGAGKTTLLNYILTEQHNRKIAV 72

Query: 62  ILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYAL 121
           ILNEFGE   VE+++     GG L EEW+EL NGC+CC+VK S ++A+E L+Q+K ++  
Sbjct: 73  ILNEFGEGSAVEKSLA-VSQGGELYEEWLELRNGCLCCSVKDSGLRAIENLMQKKGKF-- 132

Query: 122 AIISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLDS 181
                                     D+ILLETTGLA+P  +AS+ W+D +L S I LD 
Sbjct: 133 --------------------------DYILLETTGLADPGAVASMFWVDAELGSDIYLDG 192

Query: 182 IITVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYEI 241
           IITVVD+K     L E +      EA  Q+A AD I++NK DLVS E     L  L   I
Sbjct: 193 IITVVDSKYGLKHLTEEKPDGLVNEATRQVALADMILINKTDLVSEEE----LNNLRTTI 252

Query: 242 RNINSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTLC 301
           R+IN L K++ + R +V+LS IL+ ++Y+  +   L+  L+    +ST    D  + T+ 
Sbjct: 253 RSINGLGKVLETQRSRVHLSNILDLHAYDILSGISLQKKLQH---VSTAPHLDQSIVTVT 312

Query: 302 ISDHDKVDLDKVHSWLEEILWEK----KGG--MDVYRCKGVLSIKNSDKLHTLQAVRELY 361
                    + ++ +++ +LWEK    K G  M+V R KG++SIK+  +   +Q + ELY
Sbjct: 313 FEVPGSAKEECLNVFIQNLLWEKNVKNKDGHCMEVIRLKGLVSIKDKPQQMIVQGIHELY 370

Query: 362 EIVPTR-QWNNGESQMNKIVFIGRNLNEDVLSETF 390
           ++  +   W +   +  ++VFIGRNL++DVL + F
Sbjct: 373 DLEESLVNWKDDAERACQLVFIGRNLDKDVLQQLF 370

BLAST of HG10004361 vs. ExPASy Swiss-Prot
Match: Q8IUF1 (COBW domain-containing protein 2 OS=Homo sapiens OX=9606 GN=CBWD2 PE=2 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.2e-62
Identity = 146/395 (36.96%), Postives = 226/395 (57.22%), Query Frame = 0

Query: 4   EEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIAVIL 63
           EED P  V + +T +  E     G  + VT+ITGYLGAGK+TL+NYIL  QH KR+AVIL
Sbjct: 16  EEDCPELVPMETTQSEEEEKSGLGAKIPVTIITGYLGAGKTTLLNYILTEQHSKRVAVIL 75

Query: 64  NEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYALAI 123
           NEFGE   +E+++     GG L EEW+EL NGC+CC+VK + ++A+E L+Q+K ++    
Sbjct: 76  NEFGEGSALEKSLA-VSQGGELYEEWLELRNGCLCCSVKDNGLRAIENLMQKKGKF---- 135

Query: 124 ISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLDSII 183
                                   D+ILLETTGLA+P  +AS+ W+D +L S I LD II
Sbjct: 136 ------------------------DYILLETTGLADPGAVASMFWVDAELGSDIYLDGII 195

Query: 184 TVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYEIRN 243
           T+VD+K     L E +      EA  Q+A AD I++NK DLV  E     ++ L   IR+
Sbjct: 196 TIVDSKYGLKHLAEEKPDGLINEATRQVALADAILINKTDLVPEE----DVKKLRATIRS 255

Query: 244 INSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTLCIS 303
           IN L +I+ + R +V+LS +L+ +++++ +   L+  L+      T+   D  + T+   
Sbjct: 256 INGLGQILETQRSRVDLSNVLDLHAFDSLSGISLQKKLQHVP--GTQPHLDQSIVTITFE 315

Query: 304 DHDKVDLDKVHSWLEEILWEK------KGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEI 363
                  + ++ +++ +LWEK         M+V R KG++SIK+  +   +Q V ELY++
Sbjct: 316 VPGNAKEEHLNMFIQNLLWEKNVRNKDNHCMEVIRLKGLVSIKDKSQQVIVQGVHELYDL 375

Query: 364 VPTR-QWNNGESQMNKIVFIGRNLNEDVLSETFRA 392
             T   W +   + N++V +GRNL++D+L + F A
Sbjct: 376 EETPVSWKDDTERTNRLVLLGRNLDKDILKQLFIA 375

BLAST of HG10004361 vs. ExPASy Swiss-Prot
Match: Q5JTY5 (COBW domain-containing protein 3 OS=Homo sapiens OX=9606 GN=CBWD3 PE=1 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 4.7e-62
Identity = 147/395 (37.22%), Postives = 224/395 (56.71%), Query Frame = 0

Query: 4   EEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIAVIL 63
           EED P  V I +T +  E     G  + VT+ITGYLGAGK+TL+NYIL  QH KR+AVIL
Sbjct: 16  EEDCPELVPIETTQSEEEEKSGLGAKIPVTIITGYLGAGKTTLLNYILTEQHSKRVAVIL 75

Query: 64  NEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYALAI 123
           NE GE   +E+++     GG L EEW+EL NGC+CC+VK + ++A+E L+Q+K ++    
Sbjct: 76  NESGEGSALEKSLA-VSQGGELYEEWLELRNGCLCCSVKDNGLRAIENLMQKKGKF---- 135

Query: 124 ISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLDSII 183
                                   D ILLETTGLA+P  +AS+ W+D +L S I LD II
Sbjct: 136 ------------------------DDILLETTGLADPGAVASMFWVDAELGSDIYLDGII 195

Query: 184 TVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYEIRN 243
           T+VD+K     L E +      EA  Q+A AD I++NK DLV  E     ++ L   IR+
Sbjct: 196 TIVDSKYGLKHLTEEKPDGLINEATRQVALADIILINKTDLVPEE----DVKKLRTTIRS 255

Query: 244 INSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTLCIS 303
           IN L +I+ + R +V+LS +L+ +++++ +   L+  L+      T+   D  + T+   
Sbjct: 256 INGLGQILETQRSRVDLSNVLDLHAFDSLSGISLQKKLQHVP--GTQPHLDQSIVTITFE 315

Query: 304 DHDKVDLDKVHSWLEEILWEK------KGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEI 363
                  + ++ +++ +LWEK         M+V R KG++SIK+  +   +Q V ELY++
Sbjct: 316 VPGNAKEEHLNMFIQNLLWEKNVRNKDNHCMEVIRLKGLVSIKDKSQQVIVQGVHELYDL 375

Query: 364 VPTR-QWNNGESQMNKIVFIGRNLNEDVLSETFRA 392
             T   W +   + N++V IGRNL++D+L + F A
Sbjct: 376 EETPVSWKDDTERTNRLVLIGRNLDKDILKQLFIA 375

BLAST of HG10004361 vs. ExPASy TrEMBL
Match: A0A6J1HE98 (COBW domain-containing protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111462688 PE=3 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.1e-191
Identity = 347/395 (87.85%), Postives = 361/395 (91.39%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIG TITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYA 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 LAIISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLESS+KLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSVKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYE 240
           SIITVVDAKNLHFQLNEHRSSSSFPEAF+QIA+ADTIILNKVDLV+SERGDGALE LE+E
Sbjct: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFYQIAFADTIILNKVDLVTSERGDGALEDLEFE 240

Query: 241 IRNINSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTL 300
           IRNINSLAKI+HSVRCQV+LSLILNCN+YNAANTAHLE LLKES SLST+DLHDTGVRTL
Sbjct: 241 IRNINSLAKIIHSVRCQVDLSLILNCNAYNAANTAHLEELLKESRSLSTKDLHDTGVRTL 300

Query: 301 CISDHDKVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEIVPT 360
           CIS HDKVDLDKV SWLEEILW++KGGMDVYRCKGVLSIKNSD+LHTLQAVRELYEIVPT
Sbjct: 301 CISYHDKVDLDKVRSWLEEILWDRKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIVPT 360

Query: 361 RQWNNGESQMNKIVFIGRNLNEDVLSETFRACAIS 396
           RQWNNGESQMNKIVFIGRNL+EDVLSETFRACA+S
Sbjct: 361 RQWNNGESQMNKIVFIGRNLSEDVLSETFRACAVS 367

BLAST of HG10004361 vs. ExPASy TrEMBL
Match: A0A1S4DVA6 (COBW domain-containing protein 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488574 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.7e-190
Identity = 344/393 (87.53%), Postives = 357/393 (90.84%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIG T+TA ++HED GVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETVTATQQHEDGGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYA 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 LAIISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLESSIKLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYE 240
           SIITVVDAKNLHFQLNEHRSSSSFPEAFHQI +ADTIILNKVDLVSS+RGDGALE LEYE
Sbjct: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIVFADTIILNKVDLVSSDRGDGALEDLEYE 240

Query: 241 IRNINSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTL 300
           IRNINSLAKI+HSVRCQV+LSLIL+CNSYNAANTAHLEALLKES SLST+DLHDTGVRTL
Sbjct: 241 IRNINSLAKIIHSVRCQVDLSLILDCNSYNAANTAHLEALLKESRSLSTQDLHDTGVRTL 300

Query: 301 CISDHDKVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEIVPT 360
           CIS+H KVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSD+LHTLQAVRELYEIVPT
Sbjct: 301 CISEHHKVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIVPT 360

Query: 361 RQWNNGESQMNKIVFIGRNLNEDVLSETFRACA 394
           RQW+NGESQMNKIVFIGRNLNEDVLS+TFR CA
Sbjct: 361 RQWSNGESQMNKIVFIGRNLNEDVLSKTFRECA 365

BLAST of HG10004361 vs. ExPASy TrEMBL
Match: A0A6J1K8W5 (COBW domain-containing protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111492164 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.7e-190
Identity = 344/395 (87.09%), Postives = 360/395 (91.14%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIG TITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYA 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 LAIISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLESS+KLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSVKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYE 240
           SIITVVDA+NLHFQLNEHRSSSSFPEAF+QIA+ADTIILNKVDLV+SERGDGALE LE+E
Sbjct: 181 SIITVVDARNLHFQLNEHRSSSSFPEAFYQIAFADTIILNKVDLVTSERGDGALEDLEFE 240

Query: 241 IRNINSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTL 300
           IRNINSLAKI+HS+RCQV+LSLILNCN+YNAANTAHLE LLKES SLST+DLHDTGVRTL
Sbjct: 241 IRNINSLAKIIHSIRCQVDLSLILNCNAYNAANTAHLEELLKESRSLSTKDLHDTGVRTL 300

Query: 301 CISDHDKVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEIVPT 360
           CIS HDKVDLDKV SWLEEILW+KKGGMDVYRCKGVLSIKNSD+LHTLQAVRELYEIVPT
Sbjct: 301 CISYHDKVDLDKVRSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIVPT 360

Query: 361 RQWNNGESQMNKIVFIGRNLNEDVLSETFRACAIS 396
           RQWNN ESQMNKIVFIGRNL+E+VLSETFRACA+S
Sbjct: 361 RQWNNRESQMNKIVFIGRNLSENVLSETFRACAVS 367

BLAST of HG10004361 vs. ExPASy TrEMBL
Match: A0A5A7VCN1 (COBW domain-containing protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00610 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.2e-188
Identity = 344/394 (87.31%), Postives = 357/394 (90.61%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIG T+TA ++HED GVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETVTATQQHEDGGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYA 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 LAIISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLESSIKLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYE 240
           SIITVVDAKNLHFQLNEHRSSSSFPEAFHQI +ADTIILNKVDLVSS+RGDGALE LEYE
Sbjct: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIVFADTIILNKVDLVSSDRGDGALEDLEYE 240

Query: 241 IRNINSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTL 300
           IRNINSLAKI+HSVRCQV+LSLIL+CNSYNAANTAHLEALLKES SLST+DLHDTGVRTL
Sbjct: 241 IRNINSLAKIIHSVRCQVDLSLILDCNSYNAANTAHLEALLKESRSLSTQDLHDTGVRTL 300

Query: 301 CISDHDKVDLDK-VHSWLEEILWEKKGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEIVP 360
           CIS+H KVDLDK VHSWLEEILWEKKGGMDVYRCKGVLSIKNSD+LHTLQAVRELYEIVP
Sbjct: 301 CISEHHKVDLDKVVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIVP 360

Query: 361 TRQWNNGESQMNKIVFIGRNLNEDVLSETFRACA 394
           TRQW+NGESQMNKIVFIGRNLNEDVLS+TFR CA
Sbjct: 361 TRQWSNGESQMNKIVFIGRNLNEDVLSKTFRECA 366

BLAST of HG10004361 vs. ExPASy TrEMBL
Match: A0A0A0KFM6 (CobW C-terminal domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G366420 PE=3 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 2.0e-188
Identity = 340/391 (86.96%), Postives = 355/391 (90.79%), Query Frame = 0

Query: 3   DEEDPPLAVEIGSTITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIAVI 62
           ++EDPPLAVEIG TITA ++HEDDGVSVGVTVITGYLGAGKSTLVNY+LNSQHGKRIAVI
Sbjct: 2   EDEDPPLAVEIGETITAVQQHEDDGVSVGVTVITGYLGAGKSTLVNYVLNSQHGKRIAVI 61

Query: 63  LNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYALA 122
           LNEFGEEIG+ERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE     
Sbjct: 62  LNEFGEEIGIERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE----- 121

Query: 123 IISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLDSI 182
                                  RLDHILLETTGLANPAPLASVLWLDDQLESSIKLDSI
Sbjct: 122 -----------------------RLDHILLETTGLANPAPLASVLWLDDQLESSIKLDSI 181

Query: 183 ITVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYEIR 242
           ITVVDAKNLHFQLNEHRSSSSFPEAFHQI +ADTIILNKVDLVSS+RGDGALE LEYEIR
Sbjct: 182 ITVVDAKNLHFQLNEHRSSSSFPEAFHQIVFADTIILNKVDLVSSDRGDGALEDLEYEIR 241

Query: 243 NINSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTLCI 302
           NINSLAKI+HSVRCQV+LSLIL+CNSYNAANTAHLEALLKES SLST+DLHDTGVRTLCI
Sbjct: 242 NINSLAKIIHSVRCQVDLSLILDCNSYNAANTAHLEALLKESRSLSTQDLHDTGVRTLCI 301

Query: 303 SDHDKVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEIVPTRQ 362
           S+ DKVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSD+LHTLQAVRELYEIVPTRQ
Sbjct: 302 SEDDKVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIVPTRQ 361

Query: 363 WNNGESQMNKIVFIGRNLNEDVLSETFRACA 394
           WNNGESQ NKIVFIGRNLNEDVLS+TFR CA
Sbjct: 362 WNNGESQTNKIVFIGRNLNEDVLSKTFRECA 364

BLAST of HG10004361 vs. TAIR 10
Match: AT1G26520.1 (Cobalamin biosynthesis CobW-like protein )

HSP 1 Score: 502.3 bits (1292), Expect = 3.6e-142
Identity = 257/392 (65.56%), Postives = 307/392 (78.32%), Query Frame = 0

Query: 2   EDEEDPPLAVEIGSTITAAERH-EDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 61
           +D+E+PP+AV+I   ++  + H   D VSVGV+VITGYLGAGKSTLVNYILN +HGKRIA
Sbjct: 13  DDDEEPPMAVQIHPDVSVGKVHSSSDTVSVGVSVITGYLGAGKSTLVNYILNGKHGKRIA 72

Query: 62  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYA 121
           VILNEFGEEIGVERAMINEG+ GA+VEEWVELANGC+CCTVKHSLVQALEQLVQRK+   
Sbjct: 73  VILNEFGEEIGVERAMINEGEEGAIVEEWVELANGCVCCTVKHSLVQALEQLVQRKD--- 132

Query: 122 LAIISEFNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 181
                                    RLDHILLETTGLANPAPLAS+LWLDDQLES +KLD
Sbjct: 133 -------------------------RLDHILLETTGLANPAPLASILWLDDQLESEVKLD 192

Query: 182 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYE 241
            I+TVVDAKNL FQLNE R SSSFPEAF+QIA+ADTII+NKVDL+S E  D     LE E
Sbjct: 193 CIVTVVDAKNLRFQLNERRDSSSFPEAFNQIAFADTIIMNKVDLISQEESD----ELEKE 252

Query: 242 IRNINSLAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRDLHDTGVRTL 301
           I +INSLA ++ SVRCQV+LS ILNC +Y++ + + LE+LL+ + SL+T DLHD+GVRTL
Sbjct: 253 IHSINSLANVIRSVRCQVDLSNILNCQAYDSTHVSRLESLLEANKSLTTTDLHDSGVRTL 312

Query: 302 CISDHDKVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEIVPT 361
           CIS+   ++LDKV  WLEEILW+KK  MDVYRCK VLSI+NSD++H LQAVR++YEIVP 
Sbjct: 313 CISEPQPINLDKVRLWLEEILWDKKSEMDVYRCKAVLSIQNSDQMHILQAVRDIYEIVPA 372

Query: 362 RQWNNGESQMNKIVFIGRNLNEDVLSETFRAC 393
           R+W+  E++ NKIVFIG  L+E+VL    R C
Sbjct: 373 RKWSEEENRTNKIVFIGHKLDEEVLRSGLRDC 372

BLAST of HG10004361 vs. TAIR 10
Match: AT1G15730.1 (Cobalamin biosynthesis CobW-like protein )

HSP 1 Score: 229.6 bits (584), Expect = 4.5e-60
Identity = 138/396 (34.85%), Postives = 211/396 (53.28%), Query Frame = 0

Query: 30  VGVTVITGYLGAGKSTLVNYILNSQHGKRIAVILNEFGEEIGVERAMINEGDGGALVEEW 89
           +  T+ITG+LG+GK+TL+N+IL   HGKRIAVI NEFG E+ ++ +++     GA  E+ 
Sbjct: 92  IPATIITGFLGSGKTTLLNHILTGDHGKRIAVIENEFG-EVDIDGSLVAAQTAGA--EDI 151

Query: 90  VELANGCICCTVKHSLVQALEQLVQRKERYALAIISEFNGASVKNTLMIQLFLHVSRLDH 149
           + L NGC+CCTV+  LV+ + ++VQ K+                            R DH
Sbjct: 152 MMLNNGCLCCTVRGDLVRMISEMVQTKK---------------------------GRFDH 211

Query: 150 ILLETTGLANPAPLASVLWLDDQLESSIKLDSIITVVDAKNLHFQLNEHRSSSSFPEAFH 209
           I++ETTGLANPAP+    + +D++ + +KLD ++T+VDAK+    L+E +      EA  
Sbjct: 212 IVIETTGLANPAPIIQTFYAEDEIFNDVKLDGVVTLVDAKHARLHLDEVKPEGYVNEAVE 271

Query: 210 QIAYADTIILNKVDLVSSERGDGALEGLEYEIRNINSLAKIVHSVRCQVNLSLILNCNSY 269
           QIAYAD II+NK DLV    G+  L  +   I+ INS+A +  +   +V+L  +L    +
Sbjct: 272 QIAYADRIIVNKTDLV----GEPELASVMQRIKTINSMAHMKRTKYGKVDLDYVLGIGGF 331

Query: 270 NAANTAHLEALLKESGS-------------------------------LSTRDLHDTGVR 329
              +   +E+ + E                                   S    HD GV 
Sbjct: 332 ---DLERIESSVNEEEKEDREGHDDHHHGHDCHDHHNEHEHEHEHEHHHSHDHTHDPGVG 391

Query: 330 TLCISDHDKVDLDKVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDKLHTLQAVRELYEIV 389
           ++ I     +DL+K + WL  +L+++    D+YR KG+LS+++ D+    Q V E++E  
Sbjct: 392 SVSIVCEGDLDLEKANMWLGALLYQR--SEDIYRMKGILSVQDMDERFVFQGVHEIFEGS 448

Query: 390 PTRQWNNGESQMNKIVFIGRNLNEDVLSETFRACAI 395
           P R W   E++ NKIVFIG+NLN + L   FRAC I
Sbjct: 452 PDRLWRKDETRTNKIVFIGKNLNREELEMGFRACLI 448

BLAST of HG10004361 vs. TAIR 10
Match: AT1G80480.1 (plastid transcriptionally active 17 )

HSP 1 Score: 223.8 bits (569), Expect = 2.5e-58
Identity = 139/418 (33.25%), Postives = 216/418 (51.67%), Query Frame = 0

Query: 15  STITAAERHEDDGVS--------VGVTVITGYLGAGKSTLVNYILNSQHGKRIAVILNEF 74
           S   +A + ED  V+        +  T+ITG+LG+GK+TL+N+IL   HGKRIAVI NEF
Sbjct: 66  SVSASATQTEDSDVTTKIPPDNRIPATIITGFLGSGKTTLLNHILTRDHGKRIAVIENEF 125

Query: 75  GEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYALAIISE 134
           G E+ ++ +++     GA  E+ V L NGC+CCTV+  LV+ + +LV  K+         
Sbjct: 126 G-EVDIDGSLVASKSIGA--EDIVMLNNGCLCCTVRGDLVRMIGELVNTKK--------- 185

Query: 135 FNGASVKNTLMIQLFLHVSRLDHILLETTGLANPAPLASVLWLDDQLESSIKLDSIITVV 194
                              + DHI++ETTGLANPAP+    + ++++ + +KLD ++T+V
Sbjct: 186 ------------------GKFDHIVIETTGLANPAPIIQTFYAEEEIFNDVKLDGVVTLV 245

Query: 195 DAKNLHFQLNEHRSSSSFPEAFHQIAYADTIILNKVDLVSSERGDGALEGLEYEIRNINS 254
           DAK+    L+E +      EA  QIAYAD II+NK DLV    G+  L  +   I+ INS
Sbjct: 246 DAKHARLHLDEVKPEGVVNEAVEQIAYADRIIVNKTDLV----GEAELGSVVQRIKTINS 305

Query: 255 LAKIVHSVRCQVNLSLILNCNSYNAANTAHLEALLKESGSLSTRD--------------- 314
           +A++  +    V+L  +L    +   +   +E+ + E       D               
Sbjct: 306 MAQMTRTKYGNVDLDYVLGIGGF---DLERIESSVNEDDKGDHHDHDHDHHHDHNHDHDH 365

Query: 315 ---------------LHDTGVRTLCISDHDKVDLDKVHSWLEEILWEKKGGMDVYRCKGV 374
                           HD GV ++ I     +DL+K + WL  +L E+    D+YR KG+
Sbjct: 366 HHHDGHDHHHHSHDHTHDPGVSSVSIVCEGSLDLEKANMWLGTLLMER--SEDIYRMKGL 425

Query: 375 LSIKNSDKLHTLQAVRELYEIVPTRQWNNGESQMNKIVFIGRNLNEDVLSETFRACAI 395
           LS+   ++    Q V ++++  P R W   E ++NKIVFIG+NLN + L + F+AC I
Sbjct: 426 LSVHTMEERFVFQGVHDIFQGSPDRLWGREEERVNKIVFIGKNLNREELEKGFKACLI 444