HG10004308 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10004308
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Asparagine synthetase [glutamine-hydrolyzing]
Location	Chr08: 15866009 .. 15869607 (+)
RNA-Seq Expression	HG10004308
Synteny	HG10004308

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTGGAATTCTCGCCGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTTCGTGTTCTCGAACTCTCTCGTCGGCAAGTGTTCTTAATCTTCTCCCTACAATTCATTCTTTATACATTCTTTCTCTCTCGTTTCATTTCTTAATCTGTATCTCATATATGCCTGATTTGAGTTGGATAATGGAACTTTTTAACTTTGTAGGATGTAGAATGTAGAAAATTACATATTAAATATAATTGTTTTGGTCAAGAATCATTTCTATTTTGACATCGGGGTTCTTTGATTTTGGCCGTTGATATTCCATAGAGGAATTCTAATGATATTCCGTTGTAATATGAAGTATGATTTTGGCCGTGTTTGGCGCGTGCTGCCATACAGTGATCTCCAGAAAACAAAAGCCCTGCCACTGATTGTGACCTTTTAATTTTAATTTTTTTTTTAACTTTTTAAATTTTTGCTAAGAATCAAATAGGATGATCCCCAGAACAATTTGAAGATAGAAAAGGAAAAATAAAACGAAAAGTTCATTAATCAACATGGCTAATCGAAGAAATTGTAATAGAATTGATATTAACTACTTCTAGTTGTTGGTTTGACAGATTGAAACATCGTGGCCCAGACTGGAGTGGGCTTTACCAACACGCAGATTGCTATTTAGCCCATCAACGTTTAGCCGTTATCGATCCTGCATCCGGTGATCAACCTCTCTATAATGAAGATAAGTCATTGTTGTTACGGTATACTCTCGGACCTTCATCGTTGACCTCTTGGTTCCTTCCCACCCGCTTTAAATTTAATTTTAGGCAGCTGCAAATATAGAAATTAAATTTAAAATATTAACATACATAACAATTTTTTTTTAAAATATTGCTATACACTTAGTTATTATTCCTAAAATGGTTACTCATTATAATTACCCTTTAATTTATTGTTATGGATGTGTTTTGTTTCATCAAATGAGAGGGGAGAAGCAGTCACACGAATACAAACCCTATCTCATCGTGTTGATGTACAATTTTTTTCTTCTTTGCTTAAAAACAGGTCAACGGAGAAATATACAACCACGAGGAGTTGAGGAAGAAATTGAGGAACCACAAAATTCGAACCGGCAGTGACTGTGATATTATCTCACATTTGGTATTAATCGATATGATTATGGAAATAATTTTTCCTCAAATAATATGATTTTCCATGAGTTACCCATTTGGTGTGGTTCAAGGGGGATGAGATTATGATATTTATCATTGGTGTCTATGTAGTATGAAGAATATGGAGAGAATTTTGTGGACATGCTCGATGGAATGTTTTCTTTCGTGCTATTGGACACACGTGACAACAGTTTCATGGCTGCTCGTGATGCAATTGGGATCACTTCCCTTTACATTGGTTGGAGACTCGATGGTAATTGGAATAATTTTGAATCTCATTTGCTCTCCATTAGTTTTAATCTAAAACTCCATAGACATTCTCTAAGTACTCTAACTTTGATTATGTCAGGCTCAGTTTGGATATCTTCGGAACTGAAAGGTTTGAATGATGATTGTGAACATTTTGAAACCTTTCCACCGGGTCACTTGTACTCTAGCAAGAAAGCTGGATTCAAAAGATGGTATAATCCAACTTGGTTCTCTGAGGATATTCCATCGACCCCATATGATCCACTTGTTCTAAGACGCGCATTGGAGAATGTATGAATTAATGTGATGGATTTGAAGACACTTTATTAAGTTTAATGTCTTTTGTTGAAATTCTATTAGGATAACGATCTTACATGCTAACTTACGCAGGCTGCGATCAAAAGGCTGATGACCGATGTCCCTTTTGGTTGCCTCTATCACAGCTCGTCACTTAGCTGGCACAAGAGCTGCCAAGCATTGGCGTACACAACTACATTCCTTCTGTGTTGGTCTCGAGGTTGGTTTTCGTTTTCTTAGTCTTGTTGTTCCTATACAATATTGATTATGTACATACTGGGAACCGTTTCTCATTGGGAATAAGATTTGTCCTATGATGTTCAGGGTTCACCAGATTTGAAGGCTGCTAGAGAAGTTGCAGACTTTTTGGGAACTGTCCACCACGAGTTCTATTTCACAGTTCAGGTACGACTTATCTAAAATTTTAGAAACAAATATACATCCCCTACTTTCCTTGACCTAATTTTACCATCTTTATCTTCTTTGATAATAGGATGGATGATGCCATTGAAGATGTATTCTATGAACTGATGAACTTTGGGCACTTGTACTTCCACAAGGCTCCTAATAAGGAAGAGTTTCACCGTGAAAGTTGTCGCAAGGTATATGATTTTATGGAGAGCATTGCTCGATTTTTGTAAGGTTATAGATGGGGGAAATTGCATGCACTCATATCAAAAACCTTTGTTTCTCGCGTGTAGATAAAGGCACTTCACATGTATGATTGCTTAAGAGCAAACAAGTCAACTTCAGCATGGGGCTTGGAAGTTCGAGTTCCATTTTTAGACAAGGAATTTATTAATGTTGCAATGGCCATTGATCCAGAATGGAAAATGGTAGAGAAATAAACTTCACAATAGCCATCAGGATTACCCTGTTTTTTTTTCCTTAAATCCTCTTAGCTCAACTTTTTGTTTCTCATCTAATCTTTTAATCTATTTCAGATCCAACATGATCAAGGTCGAATTGAAAAATGGTTTCTCAGGAAAGCTTTCGATGATGAGCAACAACCTTATCTCCCCAAGGTCTTTGAAAGAAAAACCGCTCTGCTAGTAGTATACAAAAACTATAACTTCGATAAGAATGTAACCCTTTTTCCTGTCGGCAGCATGTTTTATACAGGCAGAAGGAACAATTCAGTGATGGTGTTGGATATAGTTGGATCGATGGCCTTAACCTCTCTGCTAGTAGTATACAAAAACTATAACTTCGATAAGAATGTAACCCTTTTTCCTGTCTGCAGCATGTTTTATACAGGCAGAAGGAACAATTCAGTGATGGTGTTGGATATCGTTGGATCGATGGCCTTAAAGTCCATGCTGATCAACATGTAATTCGATAACCTCTTCCTTTCTATTGGGCCTTCGAGCCATTCGGTGTCTTTGTTGAAGACTGAAGTTTAGAAATCTAATCTTTTGGAAGTAATGTTCATATGCTTGAAGATGATTACTCAATACATGCTTTCAGGTGACTGATAAAATGATGTTGAAATGCTGAACACATCTTTCCTCAAAATACTCCAACATCAAAGGAAGCCTACTACTACAGAACCATATTTGAGAGGTTCTTCCCACAGGTAATTTACTCCTTAACACAGTGTTGGCACATGTCTCAGTGAAACTTCATTAACCTTGTTTTGCACTTTCTATGGCTGTGCAGAACTCGGCTCGTCTGACTGTCGCGGGGAGACCAAGTATCGCTTGCAGCACGGCGAAAGCTGTCGAATGGGATGCTGCTTGGTCTAAAAATCTTGATCCTTCAGGCAGAGCTGCACTTGGAGTTCATGCCTCAGCATATGGAGATGAGGCAAACGGTGTAAACGACGTTGTCCCACCCAAGATAATCGATACCATTCCGAGAATGGAGGTCATTGCACCAGAAGTAAAAATCCTTAGCTAG

mRNA sequence

ATGTGTGGAATTCTCGCCGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTCAACGGAGAAATATACAACCACGAGGAGTTGAGGAAGAAATTGAGGAACCACAAAATTCGAACCGGCAGTGACTGTGATATTATCTCACATTTGTATGAAGAATATGGAGAGAATTTTGTGGACATGCTCGATGGAATGTTTTCTTTCGTGCTATTGGACACACGTGACAACAGTTTCATGGCTGCTCGTGATGCAATTGGGATCACTTCCCTTTACATTGGTTGGAGACTCGATGGCTCAGTTTGGATATCTTCGGAACTGAAAGGTTTGAATGATGATTGTGAACATTTTGAAACCTTTCCACCGGGTCACTTGTACTCTAGCAAGAAAGCTGGATTCAAAAGATGGTATAATCCAACTTGGTTCTCTGAGGATATTCCATCGACCCCATATGATCCACTTGTTCTAAGACGCGCATTGGAGAATGCTGCGATCAAAAGGCTGATGACCGATGTCCCTTTTGGTGGTTCACCAGATTTGAAGGCTGCTAGAGAAGTTGCAGACTTTTTGGGAACTGTCCACCACGAGATGGATGATGCCATTGAAGATGTATTCTATGAACTGATGAACTTTGGGCACTTGTACTTCCACAAGGCTCCTAATAAGGAAGAGTTTCACCGTGAAAGTTGTCGCAAGATAAAGGCACTTCACATGTATGATTGCTTAAGAGCAAACAAGTCAACTTCAGCATGGGGCTTGGAAGTTCGAGTTCCATTTTTAGACAAGGAATTTATTAATGTTGCAATGGCCATTGATCCAGAATGGAAAATGATCCAACATGATCAAGGTCGAATTGAAAAATGGTTTCTCAGGAAAGCTTTCGATGATGAGCAACAACCTTATCTCCCCAAGCATGTTTTATACAGGCAGAAGGAACAATTCAGTGATGGTGTTGGATATCGTTGGATCGATGGCCTTAAAGTCCATGCTGATCAACATAACTCGGCTCGTCTGACTGTCGCGGGGAGACCAAGTATCGCTTGCAGCACGGCGAAAGCTGTCGAATGGGATGCTGCTTGGTCTAAAAATCTTGATCCTTCAGGCAGAGCTGCACTTGGAGTTCATGCCTCAGCATATGGAGATGAGGCAAACGGTGTAAACGACGTTGTCCCACCCAAGATAATCGATACCATTCCGAGAATGGAGGTCATTGCACCAGAAGTAAAAATCCTTAGCTAG

Coding sequence (CDS)

ATGTGTGGAATTCTCGCCGTTCTTGGTTGCTCCGATGATTCTCAGGCCAAACGTGTCAACGGAGAAATATACAACCACGAGGAGTTGAGGAAGAAATTGAGGAACCACAAAATTCGAACCGGCAGTGACTGTGATATTATCTCACATTTGTATGAAGAATATGGAGAGAATTTTGTGGACATGCTCGATGGAATGTTTTCTTTCGTGCTATTGGACACACGTGACAACAGTTTCATGGCTGCTCGTGATGCAATTGGGATCACTTCCCTTTACATTGGTTGGAGACTCGATGGCTCAGTTTGGATATCTTCGGAACTGAAAGGTTTGAATGATGATTGTGAACATTTTGAAACCTTTCCACCGGGTCACTTGTACTCTAGCAAGAAAGCTGGATTCAAAAGATGGTATAATCCAACTTGGTTCTCTGAGGATATTCCATCGACCCCATATGATCCACTTGTTCTAAGACGCGCATTGGAGAATGCTGCGATCAAAAGGCTGATGACCGATGTCCCTTTTGGTGGTTCACCAGATTTGAAGGCTGCTAGAGAAGTTGCAGACTTTTTGGGAACTGTCCACCACGAGATGGATGATGCCATTGAAGATGTATTCTATGAACTGATGAACTTTGGGCACTTGTACTTCCACAAGGCTCCTAATAAGGAAGAGTTTCACCGTGAAAGTTGTCGCAAGATAAAGGCACTTCACATGTATGATTGCTTAAGAGCAAACAAGTCAACTTCAGCATGGGGCTTGGAAGTTCGAGTTCCATTTTTAGACAAGGAATTTATTAATGTTGCAATGGCCATTGATCCAGAATGGAAAATGATCCAACATGATCAAGGTCGAATTGAAAAATGGTTTCTCAGGAAAGCTTTCGATGATGAGCAACAACCTTATCTCCCCAAGCATGTTTTATACAGGCAGAAGGAACAATTCAGTGATGGTGTTGGATATCGTTGGATCGATGGCCTTAAAGTCCATGCTGATCAACATAACTCGGCTCGTCTGACTGTCGCGGGGAGACCAAGTATCGCTTGCAGCACGGCGAAAGCTGTCGAATGGGATGCTGCTTGGTCTAAAAATCTTGATCCTTCAGGCAGAGCTGCACTTGGAGTTCATGCCTCAGCATATGGAGATGAGGCAAACGGTGTAAACGACGTTGTCCCACCCAAGATAATCGATACCATTCCGAGAATGGAGGTCATTGCACCAGAAGTAAAAATCCTTAGCTAG

Protein sequence

MCGILAVLGCSDDSQAKRVNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGMFSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFGGSPDLKAAREVADFLGTVHHEMDDAIEDVFYELMNFGHLYFHKAPNKEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHDQGRIEKWFLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYRWIDGLKVHADQHNSARLTVAGRPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASAYGDEANGVNDVVPPKIIDTIPRMEVIAPEVKILS

Homology

[Close]

BLAST of HG10004308 vs. NCBI nr
Match: XP_004143163.1 (asparagine synthetase [glutamine-hydrolyzing] [Cucumis sativus] >KGN47067.1 hypothetical protein Csa_020758 [Cucumis sativus])

HSP 1 Score: 704.5 bits (1817), Expect = 5.3e-199
Identity = 376/587 (64.05%), Postives = 388/587 (66.10%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSDDSQAKR                                          
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHKDCYLAHQRLAIIDPASGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNHEELRKKLRNHKIRTGSDCD+ISHLYEEYGENFVDMLDGM
Sbjct: 61  QPLYNEDQSVVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+ ARDAIGITSLYIGW LDGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK+A FKRWYNPTWFSEDIPSTPYDPLVLRRA ENA IKRLMTDVPFG           
Sbjct: 181 SSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRAFENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKAAREVADFLGTVHHE         
Sbjct: 241 VASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIEDV Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 412
           KEEFHRESCRKIKALHMYDCLRANK+TSAWGLEVRVPFLDKEFINVAMAIDPEWKMI+ D
Sbjct: 361 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRD 420

[Close]

BLAST of HG10004308 vs. NCBI nr
Match: XP_038886003.1 (asparagine synthetase [glutamine-hydrolyzing] 1-like [Benincasa hispida])

HSP 1 Score: 701.0 bits (1808), Expect = 5.9e-198
Identity = 373/587 (63.54%), Postives = 387/587 (65.93%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSDDSQAKR                                          
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADFYLSHQRLAIIDPASGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNHE+LRKKLRNHKIRTGSDCD+ISHLYEEYGENFVDMLDGM
Sbjct: 61  QPLYNEDKSIVVTVNGEIYNHEKLRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+ A DAIGITSLYIGW LDGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFIVAHDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK+AGFKRWYNPTWFSEDIPS PYDPLVLRRALENA IKRLMTDVPFG           
Sbjct: 181 SSKEAGFKRWYNPTWFSEDIPSIPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKAAREVADFLGTVHHE +       
Sbjct: 241 VASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFNFTVQGGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIEDV Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 412
           KEEFHRESCRKIKALHMYDCLRANK+TSAWGLEVRVPFLDKEFINVAMAIDPEWKMI+HD
Sbjct: 361 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKHD 420

[Close]

BLAST of HG10004308 vs. NCBI nr
Match: XP_008465262.1 (PREDICTED: asparagine synthetase [glutamine-hydrolyzing] 1-like [Cucumis melo])

HSP 1 Score: 701.0 bits (1808), Expect = 5.9e-198
Identity = 376/587 (64.05%), Postives = 389/587 (66.27%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSDDSQAKR                                          
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHKDCYLAHQRLAIIDPASGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNHEELRKKLRNHKIRTGSDCD+ISHLYEEYGENFVDMLDGM
Sbjct: 61  QPLYNEDKSIVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+ ARDAIGITSLYIGW LDGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK+A FKRWYNPTWFSEDIPSTPYDPLVLRRALENA IKRLMTDVPFG           
Sbjct: 181 SSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKAAREVADFLGTVHHE         
Sbjct: 241 VASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIEDV Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 412
           KEEFHRESCRKIKALHMYDCLRANK+TSAWGLEVRVPFLDKEFINVAMAIDPEWKMI+ D
Sbjct: 361 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRD 420

[Close]

BLAST of HG10004308 vs. NCBI nr
Match: XP_004144212.3 (asparagine synthetase [glutamine-hydrolyzing] isoform X1 [Cucumis sativus])

HSP 1 Score: 698.4 bits (1801), Expect = 3.8e-197
Identity = 372/587 (63.37%), Postives = 389/587 (66.27%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSDDSQAKR                                          
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNHEELRKKLRNHKIRT SDCD+ISHLYEEYGENFVDMLDGM
Sbjct: 61  QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+AARDAIG+TSLYIGW  DGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK+AGFKRWYNP WFSEDIPSTPYDPLVLRRALENA IKRLMTDVPFG           
Sbjct: 181 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKAAREVADFLGTVHHE         
Sbjct: 241 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIEDV Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 412
           KEEFHRESCRKIKALHMYDCLRANK+TSAWGLEVRVPFLDK+FI+VAMAIDPEWKMI+H+
Sbjct: 361 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 420

[Close]

BLAST of HG10004308 vs. NCBI nr
Match: KAE8647225.1 (hypothetical protein Csa_018945 [Cucumis sativus])

HSP 1 Score: 698.4 bits (1801), Expect = 3.8e-197
Identity = 372/587 (63.37%), Postives = 389/587 (66.27%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSDDSQAKR                                          
Sbjct: 51  MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGD 110

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNHEELRKKLRNHKIRT SDCD+ISHLYEEYGENFVDMLDGM
Sbjct: 111 QPLYNENKSIVVTVNGEIYNHEELRKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGM 170

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+AARDAIG+TSLYIGW  DGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 171 FSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLY 230

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK+AGFKRWYNP WFSEDIPSTPYDPLVLRRALENA IKRLMTDVPFG           
Sbjct: 231 SSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 290

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKAAREVADFLGTVHHE         
Sbjct: 291 VASITARYLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 350

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIEDV Y +  +                                     G+LYFHKAPN
Sbjct: 351 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPN 410

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 412
           KEEFHRESCRKIKALHMYDCLRANK+TSAWGLEVRVPFLDK+FI+VAMAIDPEWKMI+H+
Sbjct: 411 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHN 470

[Close]

BLAST of HG10004308 vs. ExPASy Swiss-Prot
Match: P49092 (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus OX=34305 GN=AS1 PE=2 SV=2)

HSP 1 Score: 591.7 bits (1524), Expect = 6.6e-168
Identity = 314/574 (54.70%), Postives = 352/574 (61.32%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSD +QAKR                                          
Sbjct: 1   MCGILAVLGCSDFTQAKRVRVLELSRRLKHRGPDWSGLHQHGDCYLAHQRLAIVDPASGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNHEELRK+L NH+ RTGSDCD+I+HLYEE+GENF+DMLDG+
Sbjct: 61  QPLFNEDKSIIVTVNGEIYNHEELRKQLPNHQFRTGSDCDVIAHLYEEHGENFMDMLDGI 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDN+F+ ARDAIG+TSLYIGW LDGSVWISSE+KGLNDDCEHFE FPPGHLY
Sbjct: 121 FSFVLLDTRDNTFIVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFEVFPPGHLY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SS++  F+RWYNPTWFSE IPS PYDPL +R A E A IKRLMTDVPFG           
Sbjct: 181 SSRERAFRRWYNPTWFSESIPSAPYDPLAVRHAFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKAA+EVAD+LGTVHHE         
Sbjct: 241 VASITSRYLATTKAAEQWGSKLHSFCVGLEGSPDLKAAKEVADYLGTVHHEFTFTVQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIE+V Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKWVISGEGSDEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 399
           KEEFH E+CRKIKALH YDCLRANKST AWGLE RVPFLDKEFINVAM IDPE+KMI+ D
Sbjct: 361 KEEFHTETCRKIKALHQYDCLRANKSTFAWGLEARVPFLDKEFINVAMNIDPEYKMIKRD 420

[Close]

BLAST of HG10004308 vs. ExPASy Swiss-Prot
Match: P49078 (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Arabidopsis thaliana OX=3702 GN=ASN1 PE=2 SV=2)

HSP 1 Score: 590.5 bits (1521), Expect = 1.5e-167
Identity = 313/576 (54.34%), Postives = 355/576 (61.63%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSDDSQAKR                                          
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGDNYLAHQRLAVIDPASGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNHEELRK+L+NHK RTGSDC++I+HLYEEYG +FVDMLDG+
Sbjct: 61  QPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVIAHLYEEYGVDFVDMLDGI 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSFM ARDAIG+TSLYIGW LDGSVWISSE+KGLNDDCEHFETFPPGH Y
Sbjct: 121 FSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFETFPPGHFY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK  GFK+WYNP WF+E +PSTPY+PL +RRA ENA IKRLMTDVPFG           
Sbjct: 181 SSKLGGFKQWYNPPWFNESVPSTPYEPLAIRRAFENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKA +EVA++LGTVHHE         
Sbjct: 241 VASITARHLAGTKAAKQWGPQLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIEDV Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 401
           K+EFH+E+CRKIKALH YDCLRANKSTSA+GLE RVPFLDK+FIN AM++DPE KMI+ +
Sbjct: 361 KKEFHQETCRKIKALHKYDCLRANKSTSAFGLEARVPFLDKDFINTAMSLDPESKMIKPE 420

[Close]

BLAST of HG10004308 vs. ExPASy Swiss-Prot
Match: O24661 (Asparagine synthetase [glutamine-hydrolyzing] OS=Triphysaria versicolor OX=64093 GN=AS PE=2 SV=3)

HSP 1 Score: 588.6 bits (1516), Expect = 5.6e-167
Identity = 317/582 (54.47%), Postives = 353/582 (60.65%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSDDSQAKR                                          
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGIHHHGDCYLAHQRLAIVDPASGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNHEELR  L NHK RTGSDCD+I+HLYEEYGENFV+MLDGM
Sbjct: 61  QPLFNEDKRIAVTVNGEIYNHEELRALLPNHKFRTGSDCDVIAHLYEEYGENFVEMLDGM 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLD+RDN+F+AARDA GITSLYIGW LDGSVWISSELKGL+D+CE+FE FPPGH+Y
Sbjct: 121 FSFVLLDSRDNTFIAARDAFGITSLYIGWGLDGSVWISSELKGLHDECENFEVFPPGHVY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK  GF+RWYNP WFSE IPSTPYDPLVLR A E A IKRLMTDVPFG           
Sbjct: 181 SSKTEGFRRWYNPPWFSEAIPSTPYDPLVLRGAFEQAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEM-------D 300
                                         GSPDLKA +EVAD+LGTVHHE         
Sbjct: 241 VAAVTARHLAGTKAAKRWGSQLHSFCVGLEGSPDLKAGKEVADYLGTVHHEFLFTVQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIEDV Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 407
           KEEFHRE+CRKIKALH YDCLRANK+TSAWGLE RVPFLDKEF+N+AM+IDPE KMI+ D
Sbjct: 361 KEEFHRETCRKIKALHQYDCLRANKATSAWGLEARVPFLDKEFVNLAMSIDPEAKMIKPD 420

[Close]

BLAST of HG10004308 vs. ExPASy Swiss-Prot
Match: P49093 (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus OX=34305 GN=AS2 PE=2 SV=2)

HSP 1 Score: 586.6 bits (1511), Expect = 2.1e-166
Identity = 323/588 (54.93%), Postives = 357/588 (60.71%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSDDSQAKR                                          
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLHQHGDNFLAHQRLAIVDPASGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEI+NHEELRK+L NHK RTG DCD+I+HLYEE+GENFVDMLDG+
Sbjct: 61  QPLFNEDQSIIVTVNGEIFNHEELRKQLPNHKFRTGCDCDVIAHLYEEHGENFVDMLDGI 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+ ARDAIG+TSLYIG+ LDGSVWI+SELKGLNDDCEHFE FPPGHLY
Sbjct: 121 FSFVLLDTRDNSFLVARDAIGVTSLYIGYGLDGSVWIASELKGLNDDCEHFELFPPGHLY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK+  F+RWYNP WFSE IPS PYDPL LR+A E A IKRLMTDVPFG           
Sbjct: 181 SSKEKEFRRWYNPPWFSEAIPSAPYDPLALRQAFEKAIIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         G+PDLKAAREVAD++GTVHHE         
Sbjct: 241 VASVTARYLADTKAAKQWGSKLHSFCVGLEGAPDLKAAREVADYIGTVHHEFQYTIQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIEDV Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEDVIYHVETYDVTTIRAGTPMFLMSRKIKSLGVKMVLSGEGSDEIFAGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 412
           KEE H+E+C KIKALH YDCLRANKST AWGLE RVPFLDK+FI+VAM IDPE KMI+ D
Sbjct: 361 KEELHQETCSKIKALHKYDCLRANKSTYAWGLEARVPFLDKKFIDVAMGIDPENKMIKRD 420

[Close]

BLAST of HG10004308 vs. ExPASy Swiss-Prot
Match: P19251 (Asparagine synthetase, nodule [glutamine-hydrolyzing] OS=Pisum sativum OX=3888 GN=AS1 PE=2 SV=3)

HSP 1 Score: 586.3 bits (1510), Expect = 2.8e-166
Identity = 317/579 (54.75%), Postives = 352/579 (60.79%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSDDSQAKR                                          
Sbjct: 1   MCGILAVLGCSDDSQAKRVRILELSRRLKHRGPDWSGLHQHGDNYLAHQRLAIVDPASGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNHEELRK+L NHK  T  DCD+I+HLYEE+GENFVDMLDG+
Sbjct: 61  QPLFNEDKSIIVTVNGEIYNHEELRKQLPNHKFFTQCDCDVIAHLYEEHGENFVDMLDGI 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+ ARDAIG+TSLYIGW LDGSVWI+SELKGLND+CEHFE FPPGHLY
Sbjct: 121 FSFVLLDTRDNSFIVARDAIGVTSLYIGWGLDGSVWIASELKGLNDECEHFEVFPPGHLY 180

Query: 181 SSKKAGFKRWYNPTWFSED-IPSTPYDPLVLRRALENAAIKRLMTDVPFG---------- 240
           SSK+  F+RWYNP WF+E  IPSTPYDPLVLR A E A IKRLMTDVPFG          
Sbjct: 181 SSKEREFRRWYNPPWFNEAIIPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSS 240

Query: 241 -------------------------------GSPDLKAAREVADFLGTVHHEMD------ 300
                                          G+PDLKA +EVADFLGTVHHE +      
Sbjct: 241 LVASVTARYLAGTKAAKQWGAKLPSFCVGLKGAPDLKAGKEVADFLGTVHHEFEFTIQDG 300

Query: 301 -DAIEDVFYELMNF-------------------------------------GHLYFHKAP 360
            DAIEDV Y    +                                     G+LYFHKAP
Sbjct: 301 IDAIEDVIYHTETYDVTTIRAATPMFLMSRKIKSSGVKWVISGEGSDEIFGGYLYFHKAP 360

Query: 361 NKEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQH 403
           N+EEFH+E+CRKIKALH YDCLRANKST AWGLE RVPFLDK+FI VAM IDPE+KMI+H
Sbjct: 361 NREEFHQETCRKIKALHRYDCLRANKSTYAWGLEARVPFLDKDFIKVAMDIDPEFKMIKH 420

[Close]

BLAST of HG10004308 vs. ExPASy TrEMBL
Match: A0A0A0KF94 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis sativus OX=3659 GN=Csa_6G183190 PE=4 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 2.6e-199
Identity = 376/587 (64.05%), Postives = 388/587 (66.10%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSDDSQAKR                                          
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHKDCYLAHQRLAIIDPASGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNHEELRKKLRNHKIRTGSDCD+ISHLYEEYGENFVDMLDGM
Sbjct: 61  QPLYNEDQSVVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+ ARDAIGITSLYIGW LDGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK+A FKRWYNPTWFSEDIPSTPYDPLVLRRA ENA IKRLMTDVPFG           
Sbjct: 181 SSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRAFENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKAAREVADFLGTVHHE         
Sbjct: 241 VASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIEDV Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 412
           KEEFHRESCRKIKALHMYDCLRANK+TSAWGLEVRVPFLDKEFINVAMAIDPEWKMI+ D
Sbjct: 361 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRD 420

[Close]

BLAST of HG10004308 vs. ExPASy TrEMBL
Match: A0A1S3CNG7 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC103502918 PE=4 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 2.8e-198
Identity = 376/587 (64.05%), Postives = 389/587 (66.27%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSDDSQAKR                                          
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHKDCYLAHQRLAIIDPASGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNHEELRKKLRNHKIRTGSDCD+ISHLYEEYGENFVDMLDGM
Sbjct: 61  QPLYNEDKSIVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+ ARDAIGITSLYIGW LDGSVWISSELKGLNDDCEHFETFPPGHLY
Sbjct: 121 FSFVLLDTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK+A FKRWYNPTWFSEDIPSTPYDPLVLRRALENA IKRLMTDVPFG           
Sbjct: 181 SSKEAKFKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKAAREVADFLGTVHHE         
Sbjct: 241 VASITARHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIEDV Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 412
           KEEFHRESCRKIKALHMYDCLRANK+TSAWGLEVRVPFLDKEFINVAMAIDPEWKMI+ D
Sbjct: 361 KEEFHRESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRD 420

[Close]

BLAST of HG10004308 vs. ExPASy TrEMBL
Match: A0A5D3CAX5 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold615G00330 PE=4 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 2.9e-195
Identity = 360/521 (69.10%), Postives = 373/521 (71.59%), Query Frame = 0


Query: 12  DDSQAKRVNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGMFSFVLL 71
           D S    VNGEIYNHEELRKKLRNHKIRTGSDCD+ISHLYEEYGENFVDMLDGMFSFVLL
Sbjct: 57  DKSIVVTVNGEIYNHEELRKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGMFSFVLL 116

Query: 72  DTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKKAG 131
           DTRDNSF+ ARDAIGITSLYIGW LDGSVWISSELKGLNDDCEHFETFPPGHLYSSK+A 
Sbjct: 117 DTRDNSFVVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKEAK 176

Query: 132 FKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------------- 191
           FKRWYNPTWFSEDIPSTPYDPLVLRRALENA IKRLMTDVPFG                 
Sbjct: 177 FKRWYNPTWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSLVASITA 236

Query: 192 ------------------------GSPDLKAAREVADFLGTVHHEMD-------DAIEDV 251
                                   GSPDLKAAREVADFLGTVHHE         DAIEDV
Sbjct: 237 RHLTGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDV 296

Query: 252 FYELMNF-------------------------------------GHLYFHKAPNKEEFHR 311
            Y +  +                                     G+LYFHKAPNKEEFHR
Sbjct: 297 IYHIETYDVTTIRASTPMYLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHR 356

Query: 312 ESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHDQGRIEK 371
           ESCRKIKALHMYDCLRANK+TSAWGLEVRVPFLDKEFINVAMAIDPEWKMI+ DQGRIEK
Sbjct: 357 ESCRKIKALHMYDCLRANKATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRDQGRIEK 416

Query: 372 WFLRKAFDDEQQPYLPKHVLYRQKEQFSDGVGYRWIDGLKVHADQH-------------- 412
           W LR+AFDDEQQPYLPKHVLYRQKEQFSDGVGY WIDGLK HA QH              
Sbjct: 417 WVLRRAFDDEQQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFP 476

[Close]

BLAST of HG10004308 vs. ExPASy TrEMBL
Match: A0A5D3BR39 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold187G00140 PE=4 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 5.2e-192
Identity = 368/614 (59.93%), Postives = 385/614 (62.70%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSDDSQAKR                                          
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRQVLKHRGPDWSGLYQHKDCYLSHQRLAIVDPAS 60

Query: 61  ---------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLD 120
                          VNGEIYNHEELRKKL+NHKIRTGSDCD+ISHLYEEYGENFVDMLD
Sbjct: 61  GDQPLYNENKSIVVTVNGEIYNHEELRKKLKNHKIRTGSDCDVISHLYEEYGENFVDMLD 120

Query: 121 GMFSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGH 180
           GMFSFVLLDTRDNSF+AARDAIG+TSLYIGW  DGSVWISSELKGLNDDCEHFETFPPGH
Sbjct: 121 GMFSFVLLDTRDNSFIAARDAIGVTSLYIGWGRDGSVWISSELKGLNDDCEHFETFPPGH 180

Query: 181 LYSSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG--------- 240
           LYSSK+AGFKRWYNP WFSEDIPSTPYDPLVLRRALENA IKRLMTDVPFG         
Sbjct: 181 LYSSKEAGFKRWYNPIWFSEDIPSTPYDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDS 240

Query: 241 ----------------------------------------------------------GS 300
                                                                     GS
Sbjct: 241 SLVASITARHLTGTRAAKHWGTQLHSFCVGLEHVFQELLFNPCNVISLHPSSFIHQFKGS 300

Query: 301 PDLKAAREVADFLGTVHHEMD-------DAIEDVFYELMNF------------------- 360
           PDLKAAREVADFLGTVHHE         DAIEDV Y +  +                   
Sbjct: 301 PDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIK 360

Query: 361 ------------------GHLYFHKAPNKEEFHRESCRKIKALHMYDCLRANKSTSAWGL 411
                             G+LYFHKAPNKEEFHRESC KIKALHMYDCLRANK+TSAWGL
Sbjct: 361 SLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHRESCHKIKALHMYDCLRANKATSAWGL 420

[Close]

BLAST of HG10004308 vs. ExPASy TrEMBL
Match: A0A6J1G8T6 (Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita moschata OX=3662 GN=LOC111451773 PE=4 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 8.9e-192
Identity = 361/587 (61.50%), Postives = 382/587 (65.08%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSDDSQAKR                                          
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHTDFYLTHQRLAIIDPASGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNHEELRKKL NHK RTGSDCD+I+HLYEEYGENFVDMLDGM
Sbjct: 61  QPLFNEDKSIVVTVNGEIYNHEELRKKLSNHKFRTGSDCDVIAHLYEEYGENFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSF+ ARDAIGITSLYIGW LDGSVWISSELKGLNDDCEHFETFPPGH Y
Sbjct: 121 FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHFY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK+ GFKRWYNPTWFSE IPSTPYDPLVLRR+LENA IKRLMTDVPFG           
Sbjct: 181 SSKEGGFKRWYNPTWFSEAIPSTPYDPLVLRRSLENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKAAREVADFLGTVHHE         
Sbjct: 241 VASITARHLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIEDV Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEDVIYHIETYDVTTIRASTPMFLMARKIKALGVKMVISGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 412
           KEEFHRESCRKIKALH+YDCLRANK+TSAWGLEVRVPFLDKEFI+ AMAIDPEWKMI+ +
Sbjct: 361 KEEFHRESCRKIKALHLYDCLRANKATSAWGLEVRVPFLDKEFIDTAMAIDPEWKMIKRE 420

[Close]

BLAST of HG10004308 vs. TAIR 10
Match: AT3G47340.1 (glutamine-dependent asparagine synthase 1 )

HSP 1 Score: 590.5 bits (1521), Expect = 1.0e-168
Identity = 313/576 (54.34%), Postives = 355/576 (61.63%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGCSDDSQAKR                                          
Sbjct: 1   MCGILAVLGCSDDSQAKRVRVLELSRRLRHRGPDWSGLYQNGDNYLAHQRLAVIDPASGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNHEELRK+L+NHK RTGSDC++I+HLYEEYG +FVDMLDG+
Sbjct: 61  QPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDCEVIAHLYEEYGVDFVDMLDGI 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           FSFVLLDTRDNSFM ARDAIG+TSLYIGW LDGSVWISSE+KGLNDDCEHFETFPPGH Y
Sbjct: 121 FSFVLLDTRDNSFMVARDAIGVTSLYIGWGLDGSVWISSEMKGLNDDCEHFETFPPGHFY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK  GFK+WYNP WF+E +PSTPY+PL +RRA ENA IKRLMTDVPFG           
Sbjct: 181 SSKLGGFKQWYNPPWFNESVPSTPYEPLAIRRAFENAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKA +EVA++LGTVHHE         
Sbjct: 241 VASITARHLAGTKAAKQWGPQLHSFCVGLEGSPDLKAGKEVAEYLGTVHHEFHFSVQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIEDV Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGADEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 401
           K+EFH+E+CRKIKALH YDCLRANKSTSA+GLE RVPFLDK+FIN AM++DPE KMI+ +
Sbjct: 361 KKEFHQETCRKIKALHKYDCLRANKSTSAFGLEARVPFLDKDFINTAMSLDPESKMIKPE 420

[Close]

BLAST of HG10004308 vs. TAIR 10
Match: AT5G10240.1 (asparagine synthetase 3 )

HSP 1 Score: 566.2 bits (1458), Expect = 2.1e-161
Identity = 297/557 (53.32%), Postives = 338/557 (60.68%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGC D+SQAKR                                          
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNH+ LR+ L++H+ RTGSDC++I+HLYEE+GE FVDMLDGM
Sbjct: 61  QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLYEEHGEEFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           F+FVLLDTRD SF+AARDAIGIT LYIGW LDGSVW +SE+K L+DDCE F  FPPGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK+ G +RWYNP WFSE +PSTPYDPLV+R   E A IKRLMTDVPFG           
Sbjct: 181 SSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKA REVAD+LGT HHE+        
Sbjct: 241 VASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFTVQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIE+V Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 382
           K+EFH E+CRKIKALH YDCLRANKSTSAWG+E RVPFLDKEFINVAM+IDPEWKMI+ D
Sbjct: 361 KKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWKMIRPD 420

[Close]

BLAST of HG10004308 vs. TAIR 10
Match: AT5G65010.1 (asparagine synthetase 2 )

HSP 1 Score: 565.1 bits (1455), Expect = 4.7e-161
Identity = 295/575 (51.30%), Postives = 345/575 (60.00%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGC D+SQAKR                                          
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNH+ LR+KL++H+ RTGSDC++I+HLYEE+GE F+DMLDGM
Sbjct: 61  QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           F+FVLLDTRD SF+AARDAIGIT LYIGW LDGSVW +SE+K L+DDCE F +FPPGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK+ G +RWYNP W++E +PSTPYDPLVLR A E A IKRLMTDVPFG           
Sbjct: 181 SSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKA REVAD+LGT HHE         
Sbjct: 241 VAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFTVQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIE+V Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 400
           K+EFH E+CRKIKALH +DCLRANKSTSAWG+E RVPFLDKEF+NVAM+IDPEWK+I+ D
Sbjct: 361 KKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWKLIKPD 420

[Close]

BLAST of HG10004308 vs. TAIR 10
Match: AT5G65010.2 (asparagine synthetase 2 )

HSP 1 Score: 564.7 bits (1454), Expect = 6.1e-161
Identity = 295/576 (51.22%), Postives = 345/576 (59.90%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGC D+SQAKR                                          
Sbjct: 1   MCGILAVLGCIDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIIDPTSGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNH+ LR+KL++H+ RTGSDC++I+HLYEE+GE F+DMLDGM
Sbjct: 61  QPLYNEDKTVAVTVNGEIYNHKILREKLKSHQFRTGSDCEVIAHLYEEHGEEFIDMLDGM 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           F+FVLLDTRD SF+AARDAIGIT LYIGW LDGSVW +SE+K L+DDCE F +FPPGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK+ G +RWYNP W++E +PSTPYDPLVLR A E A IKRLMTDVPFG           
Sbjct: 181 SSKQGGLRRWYNPPWYNEQVPSTPYDPLVLRNAFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKA REVAD+LGT HHE         
Sbjct: 241 VAAVALRHLEKSEAARQWGSQLHTFCIGLQGSPDLKAGREVADYLGTRHHEFQFTVQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIE+V Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEEVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEILGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 400
           K+EFH E+CRKIKALH +DCLRANKSTSAWG+E RVPFLDKEF+NVAM+IDPEWK+I+ D
Sbjct: 361 KKEFHEETCRKIKALHQFDCLRANKSTSAWGVEARVPFLDKEFLNVAMSIDPEWKLIKPD 420

[Close]

BLAST of HG10004308 vs. TAIR 10
Match: AT5G10240.2 (asparagine synthetase 3 )

HSP 1 Score: 555.1 bits (1429), Expect = 4.8e-158
Identity = 294/557 (52.78%), Postives = 336/557 (60.32%), Query Frame = 0


Query: 1   MCGILAVLGCSDDSQAKR------------------------------------------ 60
           MCGILAVLGC D+SQAKR                                          
Sbjct: 1   MCGILAVLGCVDNSQAKRSRIIELSRRLRHRGPDWSGLHCYEDCYLAHERLAIVDPTSGD 60

Query: 61  -------------VNGEIYNHEELRKKLRNHKIRTGSDCDIISHLYEEYGENFVDMLDGM 120
                        VNGEIYNH+ LR+ L++H+ RTGSDC++I+HL  ++GE FVDMLDGM
Sbjct: 61  QPLYNEDKTIAVTVNGEIYNHKALRENLKSHQFRTGSDCEVIAHLV-KHGEEFVDMLDGM 120

Query: 121 FSFVLLDTRDNSFMAARDAIGITSLYIGWRLDGSVWISSELKGLNDDCEHFETFPPGHLY 180
           F+FVLLDTRD SF+AARDAIGIT LYIGW LDGSVW +SE+K L+DDCE F  FPPGH+Y
Sbjct: 121 FAFVLLDTRDKSFIAARDAIGITPLYIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY 180

Query: 181 SSKKAGFKRWYNPTWFSEDIPSTPYDPLVLRRALENAAIKRLMTDVPFG----------- 240
           SSK+ G +RWYNP WFSE +PSTPYDPLV+R   E A IKRLMTDVPFG           
Sbjct: 181 SSKQGGLRRWYNPPWFSEVVPSTPYDPLVVRNTFEKAVIKRLMTDVPFGVLLSGGLDSSL 240

Query: 241 ------------------------------GSPDLKAAREVADFLGTVHHEMD------- 300
                                         GSPDLKA REVAD+LGT HHE+        
Sbjct: 241 VASVALRHLEKSEAACQWGSKLHTFCIGLKGSPDLKAGREVADYLGTRHHELHFTVQDGI 300

Query: 301 DAIEDVFYELMNF-------------------------------------GHLYFHKAPN 360
           DAIE+V Y +  +                                     G+LYFHKAPN
Sbjct: 301 DAIEEVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPN 360

Query: 361 KEEFHRESCRKIKALHMYDCLRANKSTSAWGLEVRVPFLDKEFINVAMAIDPEWKMIQHD 382
           K+EFH E+CRKIKALH YDCLRANKSTSAWG+E RVPFLDKEFINVAM+IDPEWKMI+ D
Sbjct: 361 KKEFHEETCRKIKALHQYDCLRANKSTSAWGVEARVPFLDKEFINVAMSIDPEWKMIRPD 420

The following BLAST results are available for this feature:

BLAST of HG10004308 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143163.1	5.3e-199	64.05	asparagine synthetase [glutamine-hydrolyzing] [Cucumis sativus] >KGN47067.1 hypo...	[more]
XP_038886003.1	5.9e-198	63.54	asparagine synthetase [glutamine-hydrolyzing] 1-like [Benincasa hispida]	[more]
XP_008465262.1	5.9e-198	64.05	PREDICTED: asparagine synthetase [glutamine-hydrolyzing] 1-like [Cucumis melo]	[more]
XP_004144212.3	3.8e-197	63.37	asparagine synthetase [glutamine-hydrolyzing] isoform X1 [Cucumis sativus]	[more]
KAE8647225.1	3.8e-197	63.37	hypothetical protein Csa_018945 [Cucumis sativus]	[more]

BLAST of HG10004308 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49092	6.6e-168	54.70	Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus OX=34305 GN=A...	[more]
P49078	1.5e-167	54.34	Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Arabidopsis thaliana OX=3702 ...	[more]
O24661	5.6e-167	54.47	Asparagine synthetase [glutamine-hydrolyzing] OS=Triphysaria versicolor OX=64093...	[more]
P49093	2.1e-166	54.93	Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Lotus japonicus OX=34305 GN=A...	[more]
P19251	2.8e-166	54.75	Asparagine synthetase, nodule [glutamine-hydrolyzing] OS=Pisum sativum OX=3888 G...	[more]

BLAST of HG10004308 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KF94	2.6e-199	64.05	Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis sativus OX=3659 GN=Csa_...	[more]
A0A1S3CNG7	2.8e-198	64.05	Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo OX=3656 GN=LOC1035...	[more]
A0A5D3CAX5	2.9e-195	69.10	Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=119...	[more]
A0A5D3BR39	5.2e-192	59.93	Asparagine synthetase [glutamine-hydrolyzing] OS=Cucumis melo var. makuwa OX=119...	[more]
A0A6J1G8T6	8.9e-192	61.50	Asparagine synthetase [glutamine-hydrolyzing] OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of HG10004308 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G47340.1	1.0e-168	54.34	glutamine-dependent asparagine synthase 1	[more]
AT5G10240.1	2.1e-161	53.32	asparagine synthetase 3	[more]
AT5G65010.1	4.7e-161	51.30	asparagine synthetase 2	[more]
AT5G65010.2	6.1e-161	51.22	asparagine synthetase 2	[more]
AT5G10240.2	4.8e-158	52.78	asparagine synthetase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017932	Glutamine amidotransferase type 2 domain	PFAM	PF13537	GATase_7	coord: 18..110 e-value: 2.1E-29 score: 101.9
IPR017932	Glutamine amidotransferase type 2 domain	PROSITE	PS51278	GATASE_TYPE_2	coord: 1..130 score: 17.42971
IPR029055	Nucleophile aminohydrolases, N-terminal	GENE3D	3.60.20.10	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	coord: 11..137 e-value: 1.1E-35 score: 124.6
IPR029055	Nucleophile aminohydrolases, N-terminal	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 1..140
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 206..351 e-value: 8.7E-39 score: 135.9
IPR001962	Asparagine synthase	PFAM	PF00733	Asn_synthase	coord: 216..335 e-value: 3.1E-35 score: 122.5
IPR001962	Asparagine synthase	CDD	cd01991	Asn_Synthase_B_C	coord: 156..316 e-value: 3.01688E-28 score: 110.201
None	No IPR available	PIRSR	PIRSR001589-1	PIRSR001589-1	coord: 1..18 e-value: 0.72 score: 6.1
None	No IPR available	PANTHER	PTHR11772	ASPARAGINE SYNTHETASE	coord: 332..409 coord: 175..206
None	No IPR available	PANTHER	PTHR11772:SF43	ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]	coord: 332..409 coord: 175..206
None	No IPR available	PANTHER	PTHR11772:SF43	ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]	coord: 211..332 coord: 1..19 coord: 19..174
None	No IPR available	PANTHER	PTHR11772	ASPARAGINE SYNTHETASE	coord: 211..332 coord: 1..19 coord: 19..174
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 156..333

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10004308.1	HG10004308.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006529	asparagine biosynthetic process
molecular_function	GO:0004066	asparagine synthase (glutamine-hydrolyzing) activity
molecular_function	GO:0000166	nucleotide binding