HG10004287 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10004287 vs. NCBI nr
Match: XP_038885883.1 (neutral ceramidase 2-like [Benincasa hispida])

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 537/603 (89.05%), Postives = 553/603 (91.71%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           GDV N GINRSPSAYLMNP++ERARYA NVDKEM++LKFV+G +G +IGAFSWFPTHGTS
Sbjct: 162 GDVENGGINRSPSAYLMNPEEERARYANNVDKEMSVLKFVDGESGNSIGAFSWFPTHGTS 221

Query: 63  MSRDNKLISGDNKGAAARFFEDWVASNHTTTTSSNNTKGIVELMKKAETIKATGGKRCSK 122
           MSRDNKLISGDNKGAAARFFEDWVAS  T  TSSNNT GIVELM+KAETIKATGGKRCSK
Sbjct: 222 MSRDNKLISGDNKGAAARFFEDWVASKRTKITSSNNTSGIVELMRKAETIKATGGKRCSK 281

Query: 123 TSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVG 182
           TSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFC DSGKPCDFNRSSCHGSDLLCVG
Sbjct: 282 TSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCVG 341

Query: 183 RGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQFQKAADLFTTATEKL 242
           RGPG                          FPDEILSTKIIGERQFQKAADLFTTATEKL
Sbjct: 342 RGPG--------------------------FPDEILSTKIIGERQFQKAADLFTTATEKL 401

Query: 243 TGEIDFRHVYLNFTDIEVELDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKL 302
           TGEIDFRHVYLNFTDIEVELDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKL
Sbjct: 402 TGEIDFRHVYLNFTDIEVELDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKL 461

Query: 303 WKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGE 362
           WKKLRDSL+KPS+FQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGE
Sbjct: 462 WKKLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGE 521

Query: 363 FTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAST 422
           FTTMAGR+LREAVKETLI NGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAST
Sbjct: 522 FTTMAGRQLREAVKETLIRNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAST 581

Query: 423 LYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPPDLSSVQLRFVSDPFGESPPNGVNFG 482
           LYGPHTLSAYIQEFKKLA AIAK E I KPI  PPDLSSVQLRFV DPFGESPPNG+NFG
Sbjct: 582 LYGPHTLSAYIQEFKKLAIAIAKHENIAKPIASPPDLSSVQLRFVIDPFGESPPNGINFG 641

Query: 483 DIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDLLTEGTYAVVEKLEKQRWTPVYDDDD 542
           DIQQDIKLP NGWFKRG KQKP ATFWSANPRFDLLTEGTYAVVE+LEKQRWTPVYDDDD
Sbjct: 642 DIQQDIKLPTNGWFKRGGKQKPAATFWSANPRFDLLTEGTYAVVERLEKQRWTPVYDDDD 701

Query: 543 FSLFFKWTLDNNTLINSLATIEWDIPIDANPGVYRLRHFGSSKSTINSTNVYFTGASHAF 602
           FSLFFKWTLDNNT INS+ATIEWDIPIDA+PGVYRLRHFGSSKSTINSTN+YFTGAS AF
Sbjct: 702 FSLFFKWTLDNNTFINSIATIEWDIPIDADPGVYRLRHFGSSKSTINSTNIYFTGASQAF 738

Query: 603 AVF 606
           AVF
Sbjct: 762 AVF 738

BLAST of HG10004287 vs. NCBI nr
Match: XP_008464893.1 (PREDICTED: neutral ceramidase-like [Cucumis melo])

HSP 1 Score: 1061.2 bits (2743), Expect = 3.3e-306
Identity = 523/604 (86.59%), Postives = 552/604 (91.39%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           G+V NAGINRSPSAYLMNP++ERA+YA NVDKEM+ILKFV+G +G +IGAFSWFPTHGTS
Sbjct: 171 GEVDNAGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTS 230

Query: 63  MSRDNKLISGDNKGAAARFFEDWVASN-HTTTTSSNNTKGIVELMKKAETIKATGGKRCS 122
           MSRDNKLISGDNKGAAARFFEDW ASN  TTTT SNN   I ELMKKAE IKATGGK+CS
Sbjct: 231 MSRDNKLISGDNKGAAARFFEDWAASNGRTTTTPSNNASVIEELMKKAEKIKATGGKQCS 290

Query: 123 KTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 182
           KTSS++SKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFC DSGKPCDFNRSSCHGSDLLCV
Sbjct: 291 KTSSKSSKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCV 350

Query: 183 GRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQFQKAADLFTTATEK 242
           GRGPG                          FPDEILSTKIIGERQF KAADLFTTATEK
Sbjct: 351 GRGPG--------------------------FPDEILSTKIIGERQFLKAADLFTTATEK 410

Query: 243 LTGEIDFRHVYLNFTDIEVELDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINK 302
           LTGEIDFRHVYLNFTDIEVE+DGNNVV+TCPAALGPGFAAGTTDGPGVFGFQQGDTEINK
Sbjct: 411 LTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGPGFAAGTTDGPGVFGFQQGDTEINK 470

Query: 303 LWKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG 362
           LWK+LRDSL+KPS+FQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG
Sbjct: 471 LWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG 530

Query: 363 EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAS 422
           EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAS
Sbjct: 531 EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAS 590

Query: 423 TLYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPPDLSSVQLRFVSDPFGESPPNGVNF 482
           TLYGPHTLSAYIQEFKKLA+AIAKGEK+ KPI  PPDLSSVQLR V DPFGESPP+GVNF
Sbjct: 591 TLYGPHTLSAYIQEFKKLASAIAKGEKVAKPIASPPDLSSVQLRLVMDPFGESPPDGVNF 650

Query: 483 GDIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDLLTEGTYAVVEKLEKQRWTPVYDDD 542
           GDIQQD++LPK+GWFK G+KQKPTATFWSANPRFDLLTEGTYA+VE+LEKQRWTP YDDD
Sbjct: 651 GDIQQDVELPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDD 710

Query: 543 DFSLFFKWTLDNNTLINSLATIEWDIPIDANPGVYRLRHFGSSKSTINSTNVYFTGASHA 602
           DFS+ FKW LDN TLINSLATIEWDIPIDA+PGVYRLRHFGSS+STINSTN+YFTGAS A
Sbjct: 711 DFSVSFKWKLDNTTLINSLATIEWDIPIDADPGVYRLRHFGSSRSTINSTNIYFTGASRA 748

Query: 603 FAVF 606
           FAVF
Sbjct: 771 FAVF 748

BLAST of HG10004287 vs. NCBI nr
Match: KAG7030054.1 (Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1055.4 bits (2728), Expect = 1.8e-304
Identity = 525/604 (86.92%), Postives = 545/604 (90.23%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           G+V NAGINRSPSAYLMNP +ERARY TNVD EM+++K V GG+G ++GAFSWFPTHGTS
Sbjct: 188 GEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTS 247

Query: 63  MSRDNKLISGDNKGAAARFFEDWV-ASNHTTTTSSNNTKGIVELMKKAETIKATGGKRCS 122
           MSRDNKLISGDNKGAAARFFEDWV ASN +T  +S+N   IVELMKKAETIKATGGKRCS
Sbjct: 248 MSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDIVELMKKAETIKATGGKRCS 307

Query: 123 KTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 182
           KTSSQ+SKVRKNDGSLFV AFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV
Sbjct: 308 KTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 367

Query: 183 GRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQFQKAADLFTTATEK 242
           GRGPG                          FPDEILSTKIIGERQF KA DLFTTATE+
Sbjct: 368 GRGPG--------------------------FPDEILSTKIIGERQFLKAVDLFTTATER 427

Query: 243 LTGEIDFRHVYLNFTDIEVELDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINK 302
           LTGEIDFRHVYLNFTDIEVEL G+NVVKTCPAALGPGFAAGTTDGPGVFGFQQGDT+INK
Sbjct: 428 LTGEIDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINK 487

Query: 303 LWKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG 362
           LWKKLRDSLRKPS+FQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG
Sbjct: 488 LWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG 547

Query: 363 EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAS 422
           EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTN YSQYVAT EEYEQQRYEAAS
Sbjct: 548 EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAAS 607

Query: 423 TLYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPPDLSSVQLRFVSDPFGESPPNGVNF 482
           TLYGPHTLSAYIQEFKKLA AIAKGEKI KPI  PPDLSSVQLRFVSDPF ESPPNGVNF
Sbjct: 608 TLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNF 667

Query: 483 GDIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDLLTEGTYAVVEKLEKQRWTPVYDDD 542
           GDIQQDIKLPK GWFKRG+KQKPTA FWSANPRFDLLTEGTYAVVE+LEK+RWTPVYDDD
Sbjct: 668 GDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDD 727

Query: 543 DFSLFFKWTLDNNTLINSLATIEWDIPIDANPGVYRLRHFGSSKSTINSTNVYFTGASHA 602
           DFSL FKW LDNNT  NSLATIEWDIPIDANPGVYRLRHFGSSKS INSTN+YFTGAS A
Sbjct: 728 DFSLVFKWALDNNTFTNSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSA 765

Query: 603 FAVF 606
           FAVF
Sbjct: 788 FAVF 765

BLAST of HG10004287 vs. NCBI nr
Match: KAG6599114.1 (Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1054.3 bits (2725), Expect = 4.0e-304
Identity = 524/604 (86.75%), Postives = 545/604 (90.23%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           G+V NAGINRSPSAYLMNP +ERARY TNVD EM+++K V GG+G ++GAFSWFPTHGTS
Sbjct: 188 GEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTS 247

Query: 63  MSRDNKLISGDNKGAAARFFEDWV-ASNHTTTTSSNNTKGIVELMKKAETIKATGGKRCS 122
           MSRDNKLISGDNKGAAARFFEDWV ASN +T  +S+N   I+ELMKKAETIKATGGKRCS
Sbjct: 248 MSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDILELMKKAETIKATGGKRCS 307

Query: 123 KTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 182
           KTSSQ+SKVRKNDGSLFV AFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV
Sbjct: 308 KTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 367

Query: 183 GRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQFQKAADLFTTATEK 242
           GRGPG                          FPDEILSTKIIGERQF KA DLFTTATE+
Sbjct: 368 GRGPG--------------------------FPDEILSTKIIGERQFLKAVDLFTTATER 427

Query: 243 LTGEIDFRHVYLNFTDIEVELDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINK 302
           LTGEIDFRHVYLNFTDIEVEL G+NVVKTCPAALGPGFAAGTTDGPGVFGFQQGDT+INK
Sbjct: 428 LTGEIDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINK 487

Query: 303 LWKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG 362
           LWKKLRDSLRKPS+FQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG
Sbjct: 488 LWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG 547

Query: 363 EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAS 422
           EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTN YSQYVAT EEYEQQRYEAAS
Sbjct: 548 EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAAS 607

Query: 423 TLYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPPDLSSVQLRFVSDPFGESPPNGVNF 482
           TLYGPHTLSAYIQEFKKLA AIAKGEKI KPI  PPDLSSVQLRFVSDPF ESPPNGVNF
Sbjct: 608 TLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNF 667

Query: 483 GDIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDLLTEGTYAVVEKLEKQRWTPVYDDD 542
           GDIQQDIKLPK GWFKRG+KQKPTA FWSANPRFDLLTEGTYAVVE+LEK+RWTPVYDDD
Sbjct: 668 GDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDD 727

Query: 543 DFSLFFKWTLDNNTLINSLATIEWDIPIDANPGVYRLRHFGSSKSTINSTNVYFTGASHA 602
           DFSL FKW LDNNT  NSLATIEWDIPIDANPGVYRLRHFGSSKS INSTN+YFTGAS A
Sbjct: 728 DFSLVFKWALDNNTFTNSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSA 765

Query: 603 FAVF 606
           FAVF
Sbjct: 788 FAVF 765

BLAST of HG10004287 vs. NCBI nr
Match: XP_031742885.1 (neutral ceramidase 2 [Cucumis sativus])

HSP 1 Score: 1052.4 bits (2720), Expect = 1.5e-303
Identity = 521/604 (86.26%), Postives = 545/604 (90.23%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           G+V NAGINRSPSAYLMNP++ERA+YA NVDKEM+ILKFV+G NG +IGAFSWFPTHGTS
Sbjct: 167 GEVENAGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTS 226

Query: 63  MSRDNKLISGDNKGAAARFFEDWVASN-HTTTTSSNNTKGIVELMKKAETIKATGGKRCS 122
           MSRDNKLISGDNKGAAARFFEDW ASN  TTTT SNNT GI +LMKKA  IKATGGKRCS
Sbjct: 227 MSRDNKLISGDNKGAAARFFEDWAASNRRTTTTPSNNTSGIEDLMKKAGKIKATGGKRCS 286

Query: 123 KTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 182
           KTSS++SKVRKNDGSLFV AFCQSNVGDVTPNVLGAFCTDSG PCDFN SSCHGSDLLCV
Sbjct: 287 KTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCV 346

Query: 183 GRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQFQKAADLFTTATEK 242
           GRGPG                          FPDEILSTKIIGERQF KAADLFTTATEK
Sbjct: 347 GRGPG--------------------------FPDEILSTKIIGERQFLKAADLFTTATEK 406

Query: 243 LTGEIDFRHVYLNFTDIEVELDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINK 302
           LTGEIDFRHVYLNFTDIEV +DGNNVV+TCPAALGPGFAAGTTDGPG FGFQQGDTEINK
Sbjct: 407 LTGEIDFRHVYLNFTDIEVAIDGNNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEINK 466

Query: 303 LWKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG 362
           LWK+LRDSL+KPS+FQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLG LIILSVPG
Sbjct: 467 LWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGNLIILSVPG 526

Query: 363 EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAS 422
           EFTTMAGRRLREAVKETLISNGNG FDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAS
Sbjct: 527 EFTTMAGRRLREAVKETLISNGNGVFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAS 586

Query: 423 TLYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPPDLSSVQLRFVSDPFGESPPNGVNF 482
           TLYGPHTLSAYIQEFKKLA AIAKGEKI KPI  PPDLSSVQLR V DPFGESPPNGVNF
Sbjct: 587 TLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGESPPNGVNF 646

Query: 483 GDIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDLLTEGTYAVVEKLEKQRWTPVYDDD 542
           GDIQQD++LPK+GWFK G+KQKPTATFWSANPRFDLLTEGTYA+VE+LEKQRWTP YDDD
Sbjct: 647 GDIQQDVELPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDD 706

Query: 543 DFSLFFKWTLDNNTLINSLATIEWDIPIDANPGVYRLRHFGSSKSTINSTNVYFTGASHA 602
           DFSLFFKW LD NT IN+L TIEWDIPIDA+PGVYRLRHFGSS+STINSTNVYFTGAS A
Sbjct: 707 DFSLFFKWKLD-NTFINNLVTIEWDIPIDADPGVYRLRHFGSSRSTINSTNVYFTGASRA 743

Query: 603 FAVF 606
           FAVF
Sbjct: 767 FAVF 743

BLAST of HG10004287 vs. ExPASy Swiss-Prot
Match: Q304B9 (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 4.2e-179
Identity = 335/619 (54.12%), Postives = 418/619 (67.53%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           GD+ +AG+NRSPS+YL NP  ER++Y  +VDKEMT++KFV+   G   G+F+WF THGTS
Sbjct: 171 GDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPT-GSFNWFATHGTS 230

Query: 63  MSRDNKLISGDNKGAAARFFEDWVASNHTTTTSSNN------------TKGIVELMKKAE 122
           MSR N LISGDNKGAAARF EDW  +    + SS N            ++    L+  A 
Sbjct: 231 MSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRNIPRRVSTIVSDFSRNRDRLLDIAA 290

Query: 123 TIKATGGKRCSKTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNR 182
           T K++ G    K+    ++VR      FV+AFCQSN GDV+PN LG FC D+G PCDFN 
Sbjct: 291 TYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNH 350

Query: 183 SSCHGSDLLCVGRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQFQK 242
           S+C+G + LC GRGPGY                          PDE  ST+IIGE+QF+ 
Sbjct: 351 STCNGQNELCYGRGPGY--------------------------PDEFESTRIIGEKQFKM 410

Query: 243 AADLFTTATEKLTGEIDFRHVYLNFTDIEVEL----DGNNVVKTCPAALGPGFAAGTTDG 302
           A +LF  ATEKL G+I ++H YL+F++++V +     G+  VKTCPAA+G GFAAGTTDG
Sbjct: 411 AVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDG 470

Query: 303 PGVFGFQQGDTEINKLWKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAILPI 362
           PG F F+QGD + N  W+ +R+ LR P   QV CQKPK +LLDTGEM EPY WAP+ILPI
Sbjct: 471 PGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPI 530

Query: 363 QILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYV 422
           QILR+G+L+ILSVPGEFTTMAGRRLR+A+K  LIS+   EF ++ HVVIAGLTNTYSQY+
Sbjct: 531 QILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYI 590

Query: 423 ATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPPDLSSVQLRF 482
           ATFEEYE QRYE ASTLYG HTL+AYIQEFKKLATA+  G  + +    PPDL   Q+  
Sbjct: 591 ATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPRG-PQPPDLLDKQISL 650

Query: 483 VSDPFGESPPNGVNFGDIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDLLTEGTYAVV 542
           +S    +S P GV FGD++ D+  P    F+RG  Q+  ATFWS  PR DL+TEG++AVV
Sbjct: 651 LSPVVVDSTPLGVKFGDVKADV--PPKSTFRRG--QQVNATFWSGCPRNDLMTEGSFAVV 710

Query: 543 EKL-EKQRWTPVYDDDDFSLFFKWTLDNNTLINSLATIEWDIPIDANPGVYRLRHFGSSK 602
           E L E  +W PVYDDDDFSL FKW+        S ATIEW +P  A  GVYR+RH+G+SK
Sbjct: 711 ETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASK 756

Query: 603 STINSTNVYFTGASHAFAV 605
           S   S +  F+G+S AF V
Sbjct: 771 SLFGSIS-SFSGSSSAFVV 756

BLAST of HG10004287 vs. ExPASy Swiss-Prot
Match: Q0JL46 (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 1.3e-175
Identity = 328/633 (51.82%), Postives = 423/633 (66.82%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           GD+ +AG+NRSPSAYL NP +ER++Y  NVDKEMT++KFV+   G  +G+F+WF THGTS
Sbjct: 186 GDLLDAGVNRSPSAYLNNPAEERSKYEYNVDKEMTLIKFVDDELGP-VGSFNWFATHGTS 245

Query: 63  MSRDNKLISGDNKGAAARFFEDW-----------------VASNHTTTTSSNNTKGIV-- 122
           MSR N LISGDNKGAAARF EDW                 + S H T+        I+  
Sbjct: 246 MSRTNSLISGDNKGAAARFMEDWAEQMGLPKQSAHANSDDLRSLHKTSVLPRRVSTIIPE 305

Query: 123 ------ELMKKAETIKATGGKRCSKTS-SQASKVRKNDGSLFVAAFCQSNVGDVTPNVLG 182
                 +L++ A + +A+GG+R + +S ++  +  + +   FV+AFCQSN GDV+PNVLG
Sbjct: 306 PNEITDDLIQLASSYEASGGRRLAGSSITRRIRSTQQNKPKFVSAFCQSNCGDVSPNVLG 365

Query: 183 AFCTDSGKPCDFNRSSCHGSDLLCVGRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDE 242
            FC D+  PCDFN S+C+G + LC GRGPGY                          PDE
Sbjct: 366 TFCIDTNLPCDFNHSTCNGKNELCYGRGPGY--------------------------PDE 425

Query: 243 ILSTKIIGERQFQKAADLFTTATEKLTGEIDFRHVYLNFTDIEVELD----GNNVVKTCP 302
             ST++IG RQF KA DLF +A+E++ G+ID+RH YL+F+ +EV++     G   VKTCP
Sbjct: 426 FESTRVIGNRQFLKARDLFDSASEEIQGKIDYRHTYLDFSKLEVKVSTSAGGQQTVKTCP 485

Query: 303 AALGPGFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLRKPSDFQVGCQKPKTVLLDTGE 362
           AA+G  FAAGTTDGPG F F+QGD + N  WK +R+ L+ P   QV C  PK +LLDTGE
Sbjct: 486 AAMGFAFAAGTTDGPGAFDFRQGDVKGNPFWKLVRNLLKTPGKDQVECHSPKPILLDTGE 545

Query: 363 MFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTH 422
           M EPY WAPAILP+Q++R+G+L+IL VPGEFTTMAGRRLR+AVK T++++GN EFD + H
Sbjct: 546 MKEPYDWAPAILPVQMIRIGQLVILCVPGEFTTMAGRRLRDAVK-TVLTSGNSEFDKNIH 605

Query: 423 VVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKP 482
           VV+AGLTN+YSQY+ TFEEY+ QRYE ASTLYGPHTLSAYIQEF+KLA A+   +++   
Sbjct: 606 VVLAGLTNSYSQYITTFEEYQIQRYEGASTLYGPHTLSAYIQEFQKLAMAMIANKEVPTN 665

Query: 483 ITLPPDLSSVQLRFVSDPFGESPPNGVNFGDIQQDIKLPKNGWFKRGNKQKPTATFWSAN 542
              PPD+   Q+  +     +S P GV FGD+  D+  P N  F +G+     ATF+SA 
Sbjct: 666 FQ-PPDMLDKQIGLLPGVVFDSTPLGVKFGDVNSDV--PGNSTFNKGS--TVNATFYSAC 725

Query: 543 PRFDLLTEGTYAVVEKLE-KQRWTPVYDDDDFSLFFKWTLDNNTLINSLATIEWDIPIDA 602
           PR DLLT+GT+A+VEKL+    W PVYDDDD+SL FKW+        S AT+EW +P DA
Sbjct: 726 PRNDLLTDGTFALVEKLDGNNNWVPVYDDDDWSLRFKWSRPARLSSRSFATLEWTVPEDA 784

Query: 603 NPGVYRLRHFGSSKSTINSTNVYFTGASHAFAV 605
             GVYRLRHFG+SK    S   +FTG S AFAV
Sbjct: 786 AAGVYRLRHFGASKPMFGSVR-HFTGTSRAFAV 784

BLAST of HG10004287 vs. ExPASy Swiss-Prot
Match: F4HQM3 (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 1.9e-171
Identity = 327/629 (51.99%), Postives = 417/629 (66.30%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           G++ +AG+NRSPSAYL NP +ER+++  NVDKEMT+LKFV+   G  +G+F+WF THGTS
Sbjct: 182 GELLDAGVNRSPSAYLNNPSKERSKHKYNVDKEMTLLKFVDDQWGP-VGSFNWFATHGTS 241

Query: 63  MSRDNKLISGDNKGAAARFFEDWVASN-----------------HTTTTSSNNTKGIVEL 122
           MSR N LISGDNKGAA+RF EDW   N                   ++   N+     EL
Sbjct: 242 MSRTNSLISGDNKGAASRFMEDWYEQNTAERSYSEEFISDEIPRRVSSLIENHQDSHHEL 301

Query: 123 MKKAETIKATGGKRCSKTSSQASKV----RKNDGSLFVAAFCQSNVGDVTPNVLGAFCTD 182
           ++ A   ++  GK  ++ SS A +V    RK D   FV+AFCQ+N GDV+PNVLGAFC D
Sbjct: 302 LELASYFESQPGKPVTRISSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLD 361

Query: 183 SGKPCDFNRSSCHGSDLLCVGRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTK 242
           +G PCDFN S+C G + +C GRGPGY                          PDE  ST+
Sbjct: 362 TGLPCDFNHSTCGGKNEMCYGRGPGY--------------------------PDEFESTR 421

Query: 243 IIGERQFQKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEL---DG-NNVVKTCPAALGP 302
           IIGERQF+ A +LF  A+E+L G++D+RHVY++F+ + V L   DG + VVKTCPAA+G 
Sbjct: 422 IIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGF 481

Query: 303 GFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPY 362
            FAAGTTDGPG F F QGD + N  W+ +R+ L+ P   Q+ C  PK +LLDTGEM +PY
Sbjct: 482 AFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPY 541

Query: 363 AWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAG 422
            WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK  L ++GN +   + HVVIAG
Sbjct: 542 DWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHVVIAG 601

Query: 423 LTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPP 482
           L N YSQYV TFEEY+ QRYE ASTLYGPHTLS YIQEFKKL+ ++   +  V+P   PP
Sbjct: 602 LANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVL-DMPVQPGPQPP 661

Query: 483 DLSSVQLRFVSDPFGESPPNGVNFGDIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDL 542
           DL   QL F++    ++ P+G +FGD+  D+  PKN   KRGN Q  T  F SA PR DL
Sbjct: 662 DLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNGQ-VTVVFRSACPRNDL 721

Query: 543 LTEGTYAVVEKLEK--QRWTPVYDDDDFSLFFKWTLDNNTLINSLATIEWDIPIDANPGV 602
           LTEGT+ +VE+LE+  + WTPVYDDDD  L FKW+        S AT+EW IP  A+PGV
Sbjct: 722 LTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESASPGV 778

Query: 603 YRLRHFGSSKSTINSTNVYFTGASHAFAV 605
           YR+ HFG++K    S + +FTG+S AF V
Sbjct: 782 YRITHFGAAKKLFGSVH-HFTGSSSAFVV 778

BLAST of HG10004287 vs. ExPASy Swiss-Prot
Match: F4KHQ8 (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 1.0e-156
Identity = 305/623 (48.96%), Postives = 397/623 (63.72%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           G++ +AG+NRSPSAYL NP  ER++Y  +VDKEMT++KFV+                   
Sbjct: 174 GELLDAGVNRSPSAYLNNPAHERSKYEYDVDKEMTLVKFVD------------------- 233

Query: 63  MSRDNKLISGDNKGAAARFFEDWVASNH----TTTTSSNNTKGIV------ELMKKAETI 122
                     D  G  AR  EDW    +        S      I+      +LM+ A ++
Sbjct: 234 ----------DQWGPVARIMEDWFERENGCRSVDVESPRRVSSIISDPYDQDLMEMASSL 293

Query: 123 KATGGKRCSKTSSQASKVRKN----DGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDF 182
            +TGGK  ++ SS A +VR      D   FV+AFCQ+N GDV+PNVLGAFC D+G PC+F
Sbjct: 294 LSTGGKTVTRMSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEF 353

Query: 183 NRSSCHGSDLLCVGRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQF 242
           N+S+C G +  C GRGPGY                          PDE  ST+IIGERQF
Sbjct: 354 NQSTCGGKNEQCYGRGPGY--------------------------PDEFESTRIIGERQF 413

Query: 243 QKAADLFTTATEKLTGEIDFRHVYLNFTDIEVELDGNN----VVKTCPAALGPGFAAGTT 302
           +KAADLFT A+E++ G++D+RH Y++F+ +EV ++G N    VVKTCPAA+G GFAAGTT
Sbjct: 414 KKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFGFAAGTT 473

Query: 303 DGPGVFGFQQGDTEINKLWKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAIL 362
           DGPG F F+QGD + N  W+ +R+ L+ P++ QV CQ+PK +LLDTGEM +PY WAP+IL
Sbjct: 474 DGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQPYDWAPSIL 533

Query: 363 PIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQ 422
           P+QILR+G+L+IL VPGEFTTMAGRRLR+AVK  L    NG    +  VVIAGLTN+YSQ
Sbjct: 534 PVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKEGSNGR---EFSVVIAGLTNSYSQ 593

Query: 423 YVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPPDLSSVQL 482
           Y+ATFEEY+ QRYE ASTLYGPHTLS YIQEFKKLA  +   +    P   PPDL   Q+
Sbjct: 594 YIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLANDLLSAQ-TTDPGPQPPDLLHKQI 653

Query: 483 RFVSDPFGESPPNGVNFGDIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDLLTEGTYA 542
             ++    +  P G  FGD+  D+  P+   F++G        F SANPR DL+TEGT+A
Sbjct: 654 SLLTPVVADMTPIGTAFGDVTSDV--PRLSKFRKG-ADIVRVQFRSANPRNDLMTEGTFA 713

Query: 543 VVEK-LE-KQRWTPVYDDDDFSLFFKWTLDNNTLINSLATIEWDIPIDANPGVYRLRHFG 602
           +VE+ LE ++ W PVYDDDDF L FKW+        S ATIEW IP  A+PGVYR+ HFG
Sbjct: 714 LVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTATIEWRIPETASPGVYRITHFG 733

Query: 603 SSKSTINSTNVYFTGASHAFAVF 606
           S+K+ I+S + +F+G+S AF V+
Sbjct: 774 SAKTPISSIH-HFSGSSSAFVVY 733

BLAST of HG10004287 vs. ExPASy Swiss-Prot
Match: Q55G11 (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 6.6e-108
Identity = 243/610 (39.84%), Postives = 333/610 (54.59%), Query Frame = 0

Query: 2   TGDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGT 61
           TG++ N  INRSP AY  NP++E+A Y +NVDK MT+L+ +   NG    A S+F  H T
Sbjct: 191 TGELWNTNINRSPFAYDNNPEEEKAMYDSNVDKNMTVLR-IEDMNGNPFAAISFFAVHCT 250

Query: 62  SMSRDNKLISGDNKGAAARFFEDWVASNHTTTTSSNNTKGIVELMKKAETIKATGGKRCS 121
           SM+  N LISGDNKG A+  +E  V           N  G             T GK   
Sbjct: 251 SMNNTNHLISGDNKGYASYLWEKQV-----------NGPG-------------TAGK--- 310

Query: 122 KTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 181
                           FVAAF QSN GDV+PN  G  C D G PCD+  S+C+G +  C 
Sbjct: 311 --------------GPFVAAFGQSNEGDVSPNTRGPTCRD-GSPCDYKTSTCNGRNEECW 370

Query: 182 GRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQFQKAADLFTTATEK 241
             GPG                         +  D   ST+IIG  QF KA +LF  A+ +
Sbjct: 371 SLGPG-------------------------KDGDMFESTQIIGGNQFNKALELFNNASIQ 430

Query: 242 LTGEIDFRHVYLNFTDIEVELDGNNVV---KTCPAALGPGFAAGTTDGPGVFGFQQGD-- 301
           ++G + +RH ++ FT++ VE   N+ V    TC  A+G  FAAGTTDGPG F F Q D  
Sbjct: 431 VSGPVQYRHSWVQFTNVSVEPPYNSGVDNATTCRGAMGYSFAAGTTDGPGAFNFVQSDNN 490

Query: 302 TEINKLWKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLII 361
           T  N  W  + D + KP+  Q+ CQ PK +LLD G M EP  W P ++PIQI+ +G++++
Sbjct: 491 TSGNPFWNFIGDFIAKPTPDQIRCQSPKPILLDVG-MVEPIPWVPDVMPIQIVTIGQIVL 550

Query: 362 LSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQR 421
           ++VPGEFTTM+GRRLR +V+E +     GE  ++  V+IAGL+NTYS Y+ATFEEY+ QR
Sbjct: 551 VAVPGEFTTMSGRRLRNSVREII-----GESIENPIVLIAGLSNTYSGYIATFEEYQVQR 610

Query: 422 YEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPPDLSSVQLRFVSDPFGESPP 481
           YE AST++GPHTL +Y+QEF KLA +I  G  +    T P +L+   L F+     ++ P
Sbjct: 611 YEGASTVFGPHTLGSYMQEFGKLAQSIVDGTTVPAGPT-PRNLTGHTLFFLPPVIVDAAP 670

Query: 482 NGVNFGDIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDLLTEGTYAVVEKLE-KQRWT 541
           +  +FG++  D+ L           +  +  F+  NPR D + E ++  V+ L    +WT
Sbjct: 671 DFDDFGEVSIDVNL------NYSVNETVSCVFYGGNPRNDFMIESSFLSVDLLTGTDQWT 717

Query: 542 PVYDDDDFSLFFKWTLDNNTLINSLATIEWDIPIDANPGVYRLRHFGSSKSTINSTNVY- 601
            V DD D+   FKW +  + L  SL TIEW I  D  PG YR+ H G +K    S+N+  
Sbjct: 731 TVLDDGDWDTKFKWKM--HDLGFSLITIEWVIAPDTTPGTYRITHSGFAKKNPFSSNLTP 717

Query: 602 FTGASHAFAV 605
           + G S  F V
Sbjct: 791 YQGISRNFVV 717

BLAST of HG10004287 vs. ExPASy TrEMBL
Match: A0A1S3CMI6 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103502652 PE=3 SV=1)

HSP 1 Score: 1061.2 bits (2743), Expect = 1.6e-306
Identity = 523/604 (86.59%), Postives = 552/604 (91.39%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           G+V NAGINRSPSAYLMNP++ERA+YA NVDKEM+ILKFV+G +G +IGAFSWFPTHGTS
Sbjct: 171 GEVDNAGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTS 230

Query: 63  MSRDNKLISGDNKGAAARFFEDWVASN-HTTTTSSNNTKGIVELMKKAETIKATGGKRCS 122
           MSRDNKLISGDNKGAAARFFEDW ASN  TTTT SNN   I ELMKKAE IKATGGK+CS
Sbjct: 231 MSRDNKLISGDNKGAAARFFEDWAASNGRTTTTPSNNASVIEELMKKAEKIKATGGKQCS 290

Query: 123 KTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 182
           KTSS++SKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFC DSGKPCDFNRSSCHGSDLLCV
Sbjct: 291 KTSSKSSKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCV 350

Query: 183 GRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQFQKAADLFTTATEK 242
           GRGPG                          FPDEILSTKIIGERQF KAADLFTTATEK
Sbjct: 351 GRGPG--------------------------FPDEILSTKIIGERQFLKAADLFTTATEK 410

Query: 243 LTGEIDFRHVYLNFTDIEVELDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINK 302
           LTGEIDFRHVYLNFTDIEVE+DGNNVV+TCPAALGPGFAAGTTDGPGVFGFQQGDTEINK
Sbjct: 411 LTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGPGFAAGTTDGPGVFGFQQGDTEINK 470

Query: 303 LWKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG 362
           LWK+LRDSL+KPS+FQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG
Sbjct: 471 LWKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPG 530

Query: 363 EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAS 422
           EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAS
Sbjct: 531 EFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAS 590

Query: 423 TLYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPPDLSSVQLRFVSDPFGESPPNGVNF 482
           TLYGPHTLSAYIQEFKKLA+AIAKGEK+ KPI  PPDLSSVQLR V DPFGESPP+GVNF
Sbjct: 591 TLYGPHTLSAYIQEFKKLASAIAKGEKVAKPIASPPDLSSVQLRLVMDPFGESPPDGVNF 650

Query: 483 GDIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDLLTEGTYAVVEKLEKQRWTPVYDDD 542
           GDIQQD++LPK+GWFK G+KQKPTATFWSANPRFDLLTEGTYA+VE+LEKQRWTP YDDD
Sbjct: 651 GDIQQDVELPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDD 710

Query: 543 DFSLFFKWTLDNNTLINSLATIEWDIPIDANPGVYRLRHFGSSKSTINSTNVYFTGASHA 602
           DFS+ FKW LDN TLINSLATIEWDIPIDA+PGVYRLRHFGSS+STINSTN+YFTGAS A
Sbjct: 711 DFSVSFKWKLDNTTLINSLATIEWDIPIDADPGVYRLRHFGSSRSTINSTNIYFTGASRA 748

Query: 603 FAVF 606
           FAVF
Sbjct: 771 FAVF 748

BLAST of HG10004287 vs. ExPASy TrEMBL
Match: A0A0A0KII3 (Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_6G361230 PE=3 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 3.7e-303
Identity = 520/603 (86.24%), Postives = 544/603 (90.22%), Query Frame = 0

Query: 4   DVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTSM 63
           +V NAGINRSPSAYLMNP++ERA+YA NVDKEM+ILKFV+G NG +IGAFSWFPTHGTSM
Sbjct: 84  EVENAGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSM 143

Query: 64  SRDNKLISGDNKGAAARFFEDWVASN-HTTTTSSNNTKGIVELMKKAETIKATGGKRCSK 123
           SRDNKLISGDNKGAAARFFEDW ASN  TTTT SNNT GI +LMKKA  IKATGGKRCSK
Sbjct: 144 SRDNKLISGDNKGAAARFFEDWAASNRRTTTTPSNNTSGIEDLMKKAGKIKATGGKRCSK 203

Query: 124 TSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVG 183
           TSS++SKVRKNDGSLFV AFCQSNVGDVTPNVLGAFCTDSG PCDFN SSCHGSDLLCVG
Sbjct: 204 TSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVG 263

Query: 184 RGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQFQKAADLFTTATEKL 243
           RGPG                          FPDEILSTKIIGERQF KAADLFTTATEKL
Sbjct: 264 RGPG--------------------------FPDEILSTKIIGERQFLKAADLFTTATEKL 323

Query: 244 TGEIDFRHVYLNFTDIEVELDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKL 303
           TGEIDFRHVYLNFTDIEV +DGNNVV+TCPAALGPGFAAGTTDGPG FGFQQGDTEINKL
Sbjct: 324 TGEIDFRHVYLNFTDIEVAIDGNNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEINKL 383

Query: 304 WKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGE 363
           WK+LRDSL+KPS+FQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLG LIILSVPGE
Sbjct: 384 WKQLRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGNLIILSVPGE 443

Query: 364 FTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAST 423
           FTTMAGRRLREAVKETLISNGNG FDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAST
Sbjct: 444 FTTMAGRRLREAVKETLISNGNGVFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAAST 503

Query: 424 LYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPPDLSSVQLRFVSDPFGESPPNGVNFG 483
           LYGPHTLSAYIQEFKKLA AIAKGEKI KPI  PPDLSSVQLR V DPFGESPPNGVNFG
Sbjct: 504 LYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGESPPNGVNFG 563

Query: 484 DIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDLLTEGTYAVVEKLEKQRWTPVYDDDD 543
           DIQQD++LPK+GWFK G+KQKPTATFWSANPRFDLLTEGTYA+VE+LEKQRWTP YDDDD
Sbjct: 564 DIQQDVELPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDD 623

Query: 544 FSLFFKWTLDNNTLINSLATIEWDIPIDANPGVYRLRHFGSSKSTINSTNVYFTGASHAF 603
           FSLFFKW LD NT IN+L TIEWDIPIDA+PGVYRLRHFGSS+STINSTNVYFTGAS AF
Sbjct: 624 FSLFFKWKLD-NTFINNLVTIEWDIPIDADPGVYRLRHFGSSRSTINSTNVYFTGASRAF 659

Query: 604 AVF 606
           AVF
Sbjct: 684 AVF 659

BLAST of HG10004287 vs. ExPASy TrEMBL
Match: A0A5A7TA71 (Neutral ceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold119G00340 PE=3 SV=1)

HSP 1 Score: 1038.5 bits (2684), Expect = 1.1e-299
Identity = 524/645 (81.24%), Postives = 552/645 (85.58%), Query Frame = 0

Query: 4   DVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTSM 63
           +V NAGINRSPSAYLMNP++ERA+YA NVDKEM+ILKFV+G +G +IGAFSWFPTHGTSM
Sbjct: 76  EVDNAGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTSM 135

Query: 64  SRDNKLISGDNKGAAARFFEDWVASN-HTTTTSSNNTKG---------------IVELMK 123
           SRDNKLISGDNKGAAARFFEDW ASN  TTTT SNN                  I ELMK
Sbjct: 136 SRDNKLISGDNKGAAARFFEDWAASNGRTTTTPSNNASENLYNFLFLVYILKTVIEELMK 195

Query: 124 KAETIKATGGKRCSKTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCD 183
           KAE IKATGGKRCSKTSS++SKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCD
Sbjct: 196 KAEKIKATGGKRCSKTSSKSSKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCD 255

Query: 184 FNRSSCHGSDLLCVGRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQ 243
           FNRSSCHGSDLLCVGRGPG                          FPDEILSTKIIGERQ
Sbjct: 256 FNRSSCHGSDLLCVGRGPG--------------------------FPDEILSTKIIGERQ 315

Query: 244 FQKAADLFTTATEKLTGEIDFRHVYLNFTDIEVELDGNNVVKTCPAALGPGFAAGTTDGP 303
           F KAADLFTTATEKLTGEIDFRHVYLNFTDIEVE+DGNNVV+TCPAALGPGFAAGTTDGP
Sbjct: 316 FLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCPAALGPGFAAGTTDGP 375

Query: 304 GVFGFQQGDTE---------------------------INKLWKKLRDSLRKPSDFQVGC 363
           GVFGFQQGDTE                           INKLWK+LRDSL+KPS+FQVGC
Sbjct: 376 GVFGFQQGDTEVSSMADGMNLCLSFFDCLNFGFAILLQINKLWKQLRDSLKKPSEFQVGC 435

Query: 364 QKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI 423
           QKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI
Sbjct: 436 QKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI 495

Query: 424 SNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA 483
           SNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA
Sbjct: 496 SNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA 555

Query: 484 TAIAKGEKIVKPITLPPDLSSVQLRFVSDPFGESPPNGVNFGDIQQDIKLPKNGWFKRGN 543
           +AIAKGEK+ KPI  PPDLSSVQLR V DPFGESPP+GVNFGDIQQD++LPK+GWFK G+
Sbjct: 556 SAIAKGEKVAKPIASPPDLSSVQLRLVMDPFGESPPDGVNFGDIQQDVELPKDGWFKTGS 615

Query: 544 KQKPTATFWSANPRFDLLTEGTYAVVEKLEKQRWTPVYDDDDFSLFFKWTLDNNTLINSL 603
           KQKPTATFWSANPRFDLLTEGTYA+VE+LEKQRWTP YDDDDFS+ FKW LDN TLINSL
Sbjct: 616 KQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSVSFKWKLDNTTLINSL 675

Query: 604 ATIEWDIPIDANPGVYRLRHFGSSKSTINSTNVYFTGASHAFAVF 606
           ATIEWDIPIDA+PGVYRLRHFGSS+STINSTN+YFTGAS AFAVF
Sbjct: 676 ATIEWDIPIDADPGVYRLRHFGSSRSTINSTNIYFTGASRAFAVF 694

BLAST of HG10004287 vs. ExPASy TrEMBL
Match: A0A5E4FCT8 (Neutral ceramidase OS=Prunus dulcis OX=3755 GN=ALMOND_2B003982 PE=3 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 6.1e-258
Identity = 449/604 (74.34%), Postives = 513/604 (84.93%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           GDV NAGINRSPSAYL+NP +ERARY +NVD  MT+LKFV+  +GK++GAFSWF THGTS
Sbjct: 170 GDVVNAGINRSPSAYLLNPAEERARYPSNVDTLMTLLKFVDDASGKSVGAFSWFATHGTS 229

Query: 63  MSRDNKLISGDNKGAAARFFEDWV-ASNHTTTTSSNNTKGIVELMKKAETIKATGGKRCS 122
           MS++N LISGDNKGAAARFFEDW  +S+ ++TTS++++  ++ L+KKA+++KATGGK C 
Sbjct: 230 MSKNNLLISGDNKGAAARFFEDWFSSSSSSSTTSADSSASLLSLIKKAQSMKATGGKLCG 289

Query: 123 KTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 182
           KTSS+ SKVRKN+GSLFV AFCQSNVGDVTPNVLGAFCTDSG+PCDFN SSCHG+DLLC+
Sbjct: 290 KTSSRESKVRKNNGSLFVGAFCQSNVGDVTPNVLGAFCTDSGRPCDFNHSSCHGNDLLCL 349

Query: 183 GRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQFQKAADLFTTATEK 242
           GRGPGY                          PDEILSTKIIGERQFQKAA LFTTAT++
Sbjct: 350 GRGPGY--------------------------PDEILSTKIIGERQFQKAAHLFTTATQQ 409

Query: 243 LTGEIDFRHVYLNFTDIEVE-LDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEIN 302
           LTG ID+RHVYLNFTDIEVE LDGN  VKTCPAALGPGFAAGTTDGPG FGFQQGDT+IN
Sbjct: 410 LTGTIDYRHVYLNFTDIEVELLDGNKKVKTCPAALGPGFAAGTTDGPGAFGFQQGDTKIN 469

Query: 303 KLWKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVP 362
           KLW+KLRD+L+KP+ +QV CQKPKTVLLDTGEMF PYAWAPAILPIQ+LRLGKLIILSVP
Sbjct: 470 KLWRKLRDALKKPTPYQVDCQKPKTVLLDTGEMFVPYAWAPAILPIQMLRLGKLIILSVP 529

Query: 363 GEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAA 422
           GEFTTMAGRRLREAVKETLISN NGEFDD+TH+VIAGLTNTYSQY+ATFEEY QQRYEAA
Sbjct: 530 GEFTTMAGRRLREAVKETLISNSNGEFDDETHIVIAGLTNTYSQYIATFEEYAQQRYEAA 589

Query: 423 STLYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPPDLSSVQLRFVSDPFGESPPNGVN 482
           STLYGPHTLSAYIQEF+KLA A+AKGEKI K  + PPDLSSVQLR + +P G+ PP   N
Sbjct: 590 STLYGPHTLSAYIQEFEKLAKAMAKGEKITKGPS-PPDLSSVQLRLLLEPTGDLPPPHTN 649

Query: 483 FGDIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDLLTEGTYAVVEKLEKQRWTPVYDD 542
           FGD++QDI LPK+G FK+G+  +P ATFWSANPR+DLLTEGT+AVVE L+ + W PVYDD
Sbjct: 650 FGDMKQDIILPKSGSFKKGD--RPIATFWSANPRYDLLTEGTFAVVELLQGKHWVPVYDD 709

Query: 543 DDFSLFFKWTLDNNTLINSLATIEWDIPIDANPGVYRLRHFGSSKSTINSTNVYFTGASH 602
           DDF LFFKW +DN+TL  + ATIEW+IP DA  GVYRLRHFGSSK T +S N+YFTGAS 
Sbjct: 710 DDFCLFFKWKVDNSTLYGT-ATIEWEIPRDAVSGVYRLRHFGSSKKTKDSPNIYFTGASS 743

Query: 603 AFAV 605
            FAV
Sbjct: 770 GFAV 743

BLAST of HG10004287 vs. ExPASy TrEMBL
Match: A0A5E4FD18 (Neutral ceramidase OS=Prunus dulcis OX=3755 GN=ALMOND_2B003982 PE=3 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 6.1e-258
Identity = 449/604 (74.34%), Postives = 513/604 (84.93%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           GDV NAGINRSPSAYL+NP +ERARY +NVD  MT+LKFV+  +GK++GAFSWF THGTS
Sbjct: 183 GDVVNAGINRSPSAYLLNPAEERARYPSNVDTLMTLLKFVDDASGKSVGAFSWFATHGTS 242

Query: 63  MSRDNKLISGDNKGAAARFFEDWV-ASNHTTTTSSNNTKGIVELMKKAETIKATGGKRCS 122
           MS++N LISGDNKGAAARFFEDW  +S+ ++TTS++++  ++ L+KKA+++KATGGK C 
Sbjct: 243 MSKNNLLISGDNKGAAARFFEDWFSSSSSSSTTSADSSASLLSLIKKAQSMKATGGKLCG 302

Query: 123 KTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCV 182
           KTSS+ SKVRKN+GSLFV AFCQSNVGDVTPNVLGAFCTDSG+PCDFN SSCHG+DLLC+
Sbjct: 303 KTSSRESKVRKNNGSLFVGAFCQSNVGDVTPNVLGAFCTDSGRPCDFNHSSCHGNDLLCL 362

Query: 183 GRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQFQKAADLFTTATEK 242
           GRGPGY                          PDEILSTKIIGERQFQKAA LFTTAT++
Sbjct: 363 GRGPGY--------------------------PDEILSTKIIGERQFQKAAHLFTTATQQ 422

Query: 243 LTGEIDFRHVYLNFTDIEVE-LDGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEIN 302
           LTG ID+RHVYLNFTDIEVE LDGN  VKTCPAALGPGFAAGTTDGPG FGFQQGDT+IN
Sbjct: 423 LTGTIDYRHVYLNFTDIEVELLDGNKKVKTCPAALGPGFAAGTTDGPGAFGFQQGDTKIN 482

Query: 303 KLWKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVP 362
           KLW+KLRD+L+KP+ +QV CQKPKTVLLDTGEMF PYAWAPAILPIQ+LRLGKLIILSVP
Sbjct: 483 KLWRKLRDALKKPTPYQVDCQKPKTVLLDTGEMFVPYAWAPAILPIQMLRLGKLIILSVP 542

Query: 363 GEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAA 422
           GEFTTMAGRRLREAVKETLISN NGEFDD+TH+VIAGLTNTYSQY+ATFEEY QQRYEAA
Sbjct: 543 GEFTTMAGRRLREAVKETLISNSNGEFDDETHIVIAGLTNTYSQYIATFEEYAQQRYEAA 602

Query: 423 STLYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPPDLSSVQLRFVSDPFGESPPNGVN 482
           STLYGPHTLSAYIQEF+KLA A+AKGEKI K  + PPDLSSVQLR + +P G+ PP   N
Sbjct: 603 STLYGPHTLSAYIQEFEKLAKAMAKGEKITKGPS-PPDLSSVQLRLLLEPTGDLPPPHTN 662

Query: 483 FGDIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDLLTEGTYAVVEKLEKQRWTPVYDD 542
           FGD++QDI LPK+G FK+G+  +P ATFWSANPR+DLLTEGT+AVVE L+ + W PVYDD
Sbjct: 663 FGDMKQDIILPKSGSFKKGD--RPIATFWSANPRYDLLTEGTFAVVELLQGKHWVPVYDD 722

Query: 543 DDFSLFFKWTLDNNTLINSLATIEWDIPIDANPGVYRLRHFGSSKSTINSTNVYFTGASH 602
           DDF LFFKW +DN+TL  + ATIEW+IP DA  GVYRLRHFGSSK T +S N+YFTGAS 
Sbjct: 723 DDFCLFFKWKVDNSTLYGT-ATIEWEIPRDAVSGVYRLRHFGSSKKTKDSPNIYFTGASS 756

Query: 603 AFAV 605
            FAV
Sbjct: 783 GFAV 756

BLAST of HG10004287 vs. TAIR 10
Match: AT2G38010.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 629.4 bits (1622), Expect = 3.0e-180
Identity = 335/619 (54.12%), Postives = 418/619 (67.53%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           GD+ +AG+NRSPS+YL NP  ER++Y  +VDKEMT++KFV+   G   G+F+WF THGTS
Sbjct: 171 GDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPT-GSFNWFATHGTS 230

Query: 63  MSRDNKLISGDNKGAAARFFEDWVASNHTTTTSSNN------------TKGIVELMKKAE 122
           MSR N LISGDNKGAAARF EDW  +    + SS N            ++    L+  A 
Sbjct: 231 MSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRNIPRRVSTIVSDFSRNRDRLLDIAA 290

Query: 123 TIKATGGKRCSKTSSQASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNR 182
           T K++ G    K+    ++VR      FV+AFCQSN GDV+PN LG FC D+G PCDFN 
Sbjct: 291 TYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNH 350

Query: 183 SSCHGSDLLCVGRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQFQK 242
           S+C+G + LC GRGPGY                          PDE  ST+IIGE+QF+ 
Sbjct: 351 STCNGQNELCYGRGPGY--------------------------PDEFESTRIIGEKQFKM 410

Query: 243 AADLFTTATEKLTGEIDFRHVYLNFTDIEVEL----DGNNVVKTCPAALGPGFAAGTTDG 302
           A +LF  ATEKL G+I ++H YL+F++++V +     G+  VKTCPAA+G GFAAGTTDG
Sbjct: 411 AVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDG 470

Query: 303 PGVFGFQQGDTEINKLWKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAILPI 362
           PG F F+QGD + N  W+ +R+ LR P   QV CQKPK +LLDTGEM EPY WAP+ILPI
Sbjct: 471 PGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPI 530

Query: 363 QILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYV 422
           QILR+G+L+ILSVPGEFTTMAGRRLR+A+K  LIS+   EF ++ HVVIAGLTNTYSQY+
Sbjct: 531 QILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYI 590

Query: 423 ATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPPDLSSVQLRF 482
           ATFEEYE QRYE ASTLYG HTL+AYIQEFKKLATA+  G  + +    PPDL   Q+  
Sbjct: 591 ATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPRG-PQPPDLLDKQISL 650

Query: 483 VSDPFGESPPNGVNFGDIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDLLTEGTYAVV 542
           +S    +S P GV FGD++ D+  P    F+RG  Q+  ATFWS  PR DL+TEG++AVV
Sbjct: 651 LSPVVVDSTPLGVKFGDVKADV--PPKSTFRRG--QQVNATFWSGCPRNDLMTEGSFAVV 710

Query: 543 EKL-EKQRWTPVYDDDDFSLFFKWTLDNNTLINSLATIEWDIPIDANPGVYRLRHFGSSK 602
           E L E  +W PVYDDDDFSL FKW+        S ATIEW +P  A  GVYR+RH+G+SK
Sbjct: 711 ETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASK 756

Query: 603 STINSTNVYFTGASHAFAV 605
           S   S +  F+G+S AF V
Sbjct: 771 SLFGSIS-SFSGSSSAFVV 756

BLAST of HG10004287 vs. TAIR 10
Match: AT2G38010.2 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 615.9 bits (1587), Expect = 3.4e-176
Identity = 336/654 (51.38%), Postives = 417/654 (63.76%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           GD+ +AG+NRSPS+YL NP  ER++Y  +VDKEMT++KFV+   G   G+F+WF THGTS
Sbjct: 171 GDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPT-GSFNWFATHGTS 230

Query: 63  MSRDNKLISGDNKGAAARFFEDWVASNHTTTTSSNNTKGIV------------------- 122
           MSR N LISGDNKGAAARF EDW  +    + SS N    V                   
Sbjct: 231 MSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRNIPRRVSTIVSDFSRNRESYPFLEL 290

Query: 123 ----------------------------ELMKKAETIKATGGKRCSKTSSQASKVRKNDG 182
                                        L+  A T K++ G    K+    ++VR    
Sbjct: 291 PNVAILYIAVAERLMWKVMVLFYFVSESRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSK 350

Query: 183 SLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGYVTYNKILS 242
             FV+AFCQSN GDV+PN LG FC D+G PCDFN S+C+G + LC GRGPGY        
Sbjct: 351 RKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGY-------- 410

Query: 243 STNQVYAHNLFVKMPHRFPDEILSTKIIGERQFQKAADLFTTATEKLTGEIDFRHVYLNF 302
                             PDE  ST+IIGE+QF+ A +LF  ATEKL G+I ++H YL+F
Sbjct: 411 ------------------PDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDF 470

Query: 303 TDIEVEL----DGNNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLR 362
           ++++V +     G+  VKTCPAA+G GFAAGTTDGPG F F+QGD + N  W+ +R+ LR
Sbjct: 471 SNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLR 530

Query: 363 KPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRL 422
            P   QV CQKPK +LLDTGEM EPY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRL
Sbjct: 531 TPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRL 590

Query: 423 REAVKETLISNGNGEFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSA 482
           R+A+K  LIS+   EF ++ HVVIAGLTNTYSQY+ATFEEYE QRYE ASTLYG HTL+A
Sbjct: 591 RDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTA 650

Query: 483 YIQEFKKLATAIAKGEKIVKPITLPPDLSSVQLRFVSDPFGESPPNGVNFGDIQQDIKLP 542
           YIQEFKKLATA+  G  + +    PPDL   Q+  +S    +S P GV FGD++ D+  P
Sbjct: 651 YIQEFKKLATALVNGLTLPRG-PQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--P 710

Query: 543 KNGWFKRGNKQKPTATFWSANPRFDLLTEGTYAVVEKL-EKQRWTPVYDDDDFSLFFKWT 602
               F+RG  Q+  ATFWS  PR DL+TEG++AVVE L E  +W PVYDDDDFSL FKW+
Sbjct: 711 PKSTFRRG--QQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWS 770

Query: 603 LDNNTLINSLATIEWDIPIDANPGVYRLRHFGSSKSTINSTNVYFTGASHAFAV 605
                   S ATIEW +P  A  GVYR+RH+G+SKS   S +  F+G+S AF V
Sbjct: 771 RPAKLSSESQATIEWRVPESAVAGVYRIRHYGASKSLFGSIS-SFSGSSSAFVV 791

BLAST of HG10004287 vs. TAIR 10
Match: AT1G07380.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 604.0 bits (1556), Expect = 1.3e-172
Identity = 327/629 (51.99%), Postives = 417/629 (66.30%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           G++ +AG+NRSPSAYL NP +ER+++  NVDKEMT+LKFV+   G  +G+F+WF THGTS
Sbjct: 182 GELLDAGVNRSPSAYLNNPSKERSKHKYNVDKEMTLLKFVDDQWGP-VGSFNWFATHGTS 241

Query: 63  MSRDNKLISGDNKGAAARFFEDWVASN-----------------HTTTTSSNNTKGIVEL 122
           MSR N LISGDNKGAA+RF EDW   N                   ++   N+     EL
Sbjct: 242 MSRTNSLISGDNKGAASRFMEDWYEQNTAERSYSEEFISDEIPRRVSSLIENHQDSHHEL 301

Query: 123 MKKAETIKATGGKRCSKTSSQASKV----RKNDGSLFVAAFCQSNVGDVTPNVLGAFCTD 182
           ++ A   ++  GK  ++ SS A +V    RK D   FV+AFCQ+N GDV+PNVLGAFC D
Sbjct: 302 LELASYFESQPGKPVTRISSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLD 361

Query: 183 SGKPCDFNRSSCHGSDLLCVGRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTK 242
           +G PCDFN S+C G + +C GRGPGY                          PDE  ST+
Sbjct: 362 TGLPCDFNHSTCGGKNEMCYGRGPGY--------------------------PDEFESTR 421

Query: 243 IIGERQFQKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEL---DG-NNVVKTCPAALGP 302
           IIGERQF+ A +LF  A+E+L G++D+RHVY++F+ + V L   DG + VVKTCPAA+G 
Sbjct: 422 IIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGF 481

Query: 303 GFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPY 362
            FAAGTTDGPG F F QGD + N  W+ +R+ L+ P   Q+ C  PK +LLDTGEM +PY
Sbjct: 482 AFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPY 541

Query: 363 AWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAG 422
            WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK  L ++GN +   + HVVIAG
Sbjct: 542 DWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHVVIAG 601

Query: 423 LTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPP 482
           L N YSQYV TFEEY+ QRYE ASTLYGPHTLS YIQEFKKL+ ++   +  V+P   PP
Sbjct: 602 LANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVL-DMPVQPGPQPP 661

Query: 483 DLSSVQLRFVSDPFGESPPNGVNFGDIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDL 542
           DL   QL F++    ++ P+G +FGD+  D+  PKN   KRGN Q  T  F SA PR DL
Sbjct: 662 DLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNGQ-VTVVFRSACPRNDL 721

Query: 543 LTEGTYAVVEKLEK--QRWTPVYDDDDFSLFFKWTLDNNTLINSLATIEWDIPIDANPGV 602
           LTEGT+ +VE+LE+  + WTPVYDDDD  L FKW+        S AT+EW IP  A+PGV
Sbjct: 722 LTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESASPGV 778

Query: 603 YRLRHFGSSKSTINSTNVYFTGASHAFAV 605
           YR+ HFG++K    S + +FTG+S AF V
Sbjct: 782 YRITHFGAAKKLFGSVH-HFTGSSSAFVV 778

BLAST of HG10004287 vs. TAIR 10
Match: AT5G58980.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 555.1 bits (1429), Expect = 7.1e-158
Identity = 305/623 (48.96%), Postives = 397/623 (63.72%), Query Frame = 0

Query: 3   GDVANAGINRSPSAYLMNPQQERARYATNVDKEMTILKFVNGGNGKNIGAFSWFPTHGTS 62
           G++ +AG+NRSPSAYL NP  ER++Y  +VDKEMT++KFV+                   
Sbjct: 174 GELLDAGVNRSPSAYLNNPAHERSKYEYDVDKEMTLVKFVD------------------- 233

Query: 63  MSRDNKLISGDNKGAAARFFEDWVASNH----TTTTSSNNTKGIV------ELMKKAETI 122
                     D  G  AR  EDW    +        S      I+      +LM+ A ++
Sbjct: 234 ----------DQWGPVARIMEDWFERENGCRSVDVESPRRVSSIISDPYDQDLMEMASSL 293

Query: 123 KATGGKRCSKTSSQASKVRKN----DGSLFVAAFCQSNVGDVTPNVLGAFCTDSGKPCDF 182
            +TGGK  ++ SS A +VR      D   FV+AFCQ+N GDV+PNVLGAFC D+G PC+F
Sbjct: 294 LSTGGKTVTRMSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEF 353

Query: 183 NRSSCHGSDLLCVGRGPGYVTYNKILSSTNQVYAHNLFVKMPHRFPDEILSTKIIGERQF 242
           N+S+C G +  C GRGPGY                          PDE  ST+IIGERQF
Sbjct: 354 NQSTCGGKNEQCYGRGPGY--------------------------PDEFESTRIIGERQF 413

Query: 243 QKAADLFTTATEKLTGEIDFRHVYLNFTDIEVELDGNN----VVKTCPAALGPGFAAGTT 302
           +KAADLFT A+E++ G++D+RH Y++F+ +EV ++G N    VVKTCPAA+G GFAAGTT
Sbjct: 414 KKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFGFAAGTT 473

Query: 303 DGPGVFGFQQGDTEINKLWKKLRDSLRKPSDFQVGCQKPKTVLLDTGEMFEPYAWAPAIL 362
           DGPG F F+QGD + N  W+ +R+ L+ P++ QV CQ+PK +LLDTGEM +PY WAP+IL
Sbjct: 474 DGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQPYDWAPSIL 533

Query: 363 PIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNTYSQ 422
           P+QILR+G+L+IL VPGEFTTMAGRRLR+AVK  L    NG    +  VVIAGLTN+YSQ
Sbjct: 534 PVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKEGSNGR---EFSVVIAGLTNSYSQ 593

Query: 423 YVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLATAIAKGEKIVKPITLPPDLSSVQL 482
           Y+ATFEEY+ QRYE ASTLYGPHTLS YIQEFKKLA  +   +    P   PPDL   Q+
Sbjct: 594 YIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLANDLLSAQ-TTDPGPQPPDLLHKQI 653

Query: 483 RFVSDPFGESPPNGVNFGDIQQDIKLPKNGWFKRGNKQKPTATFWSANPRFDLLTEGTYA 542
             ++    +  P G  FGD+  D+  P+   F++G        F SANPR DL+TEGT+A
Sbjct: 654 SLLTPVVADMTPIGTAFGDVTSDV--PRLSKFRKG-ADIVRVQFRSANPRNDLMTEGTFA 713

Query: 543 VVEK-LE-KQRWTPVYDDDDFSLFFKWTLDNNTLINSLATIEWDIPIDANPGVYRLRHFG 602
           +VE+ LE ++ W PVYDDDDF L FKW+        S ATIEW IP  A+PGVYR+ HFG
Sbjct: 714 LVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTATIEWRIPETASPGVYRITHFG 733

Query: 603 SSKSTINSTNVYFTGASHAFAVF 606
           S+K+ I+S + +F+G+S AF V+
Sbjct: 774 SAKTPISSIH-HFSGSSSAFVVY 733