HG10004274 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10004274
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionMetallocarboxypeptidase A-like protein
LocationChr08: 15463693 .. 15467693 (+)
RNA-Seq ExpressionHG10004274
SyntenyHG10004274
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTCTAGAAATAAGGGCTATGTTGCAGAGATTCCAGTTGTTACTTATTGCCGTGGAAGGAATAACATTGATGACATGTCAAAATTCCGGATACTTCTTGTAAGTTTTGTGTTGAATTGCGTACTTATTTGATCTTCTAGACTTTTATTCCCTTGGCGTTTTTTTGAGACTGATGTTTAAGCCATGTTGAAGTGAAAGCAGCAAAGGCTTCCTGCCTGTGTACATCTTGATCTAAATTAAAATGGGCTTGATTTAGAAATTAGTTTTTCTCTGCCTTTCTTCTTTTCTTTTCCTCTCCTTAACGATTAAGGGAACATACCATCTCAGATTGGCAATTGCTGGTGTTCTTGACAGATGATTCCTCCTTGTCTTCCAGAGTTTTGGACAGCATGGAAGGGAGCTCATAACAACGGAAGTTGCTCTAAGAATTCTCTTAATCTTGAGTGAAGAACAGTTGTTGCCGCACATGGATCGAGCTTCCTTAAACAACACACTTGACAAGCTTGTCATTAAGGTATGTGTGGAGGCTGCAACTTTTTACATTGATAAGTGGAATAAGTTAATGGAGTATAAATTTTTTTGTTCTATAATTTTAGGAAATAATAGGTAGTCACACAGGACTCATGGCTAATTTAACAAACAATCAAAGTATTAGTGGGATGCTTCAACTGTCCTCCTCTTCCAGCTAACCTCTTCCTTTCTTTTTATGTAGCAATGAAGCATGCCCACATTTTGTTTTTCAACAAGAGACAACTTTTCATCGAACAAATGAAAAGAAATTAATTCTAAAAAATACAAACTCTGTAAGAGAGTGAAAATTAGAGCAGAACGGAACATTTAAAGAACCATTGGAGAGAACCAGAAAAGAACAAATAACAAAAAAGAAACTTTCTCAAAAACACCAAGCGAATGAAACAAAAGAAACTAGCAAAAGGAGAATGATAAGAACTTGAAAGAAATCTGTGCTCTAGCTGATATTGGTGCTTCAAACTTAAATCACATGTTACTGTGTGCAAAAATAATGTGCTTGCTTATGTATGTTGTCAGGTGGTGCCAATGGAAAACTTGAATGGGCGCAGGCTTGTCGAGGGCGGGGATCTATGTGAAAGAAGAAATGGTAATGCAGAAGATTTAATCCAATTATATAGTTATACAAGGTCTCCAAGGTAGATATTGACATGCCTGAGTTTGTCTACTATGGCAGGGAGAGGTGTTGATTTGAACAGAAATTGGAGTGTTGATTGGGGTAAAAAGGAAAAGGTTGGTATCTATCTTCTGATTTGCACTTGAAATCATTTGACAAGGTTTTGATATAGTCTGTAGACAGTTTCTACTGAGAAGCCAATTAGGAGAAACCATCATATTCATTTCTTGAAACAAGCTTCTTTTTTTTTTTTTTCCCTCTTTTCCAAGGCTCTATGGATTTCAGTCAGGCTTTTATGCATGATAGGTGTTGTAATACTCTCTTCTGTTCCAAGCATTTTAGTTATCCTTCTCCCTATTGCAGGATTATGATCCTTATGAGGAAAATCCTGGATTTGCACCTTTCAGCGAACCAGAAACTCAAATTATGCGAAAACTTGCTTTAACATTTGATCCACATATATGGGTCAATGTGCACTCTGGAATGGAGGTAAGAAATAATACCAATGTCATTTGTGTTTCCCTGAGTCTGAGGTGCTAAGACAACAAGATTTAGTTATTAACCTGGATTATGATCAGTGATTTTTTTTTTTTCATGAATACAGACTACAGATGCTTTCGCCTTTACTGACACCCTTTTTTCAGTTCTTTTTCTTTTAAAATCACAACTTGACAATGTGGTTGGTTCAGTTTACTCATGAAATTGTACTTCAAAGTTTCATGAAATAAATATCGATAAATTACGAGTTTAGTCAAAGACTTTGAAAATGTTCAACTAGTCTTTGAAATTTCAATTTTATGTCCTAGGTCCTTGACATATTGAAATCTTTTAAAAACTAACTAAATATAAGAGATAAAGCTAAATTTTATGTTTCATTGAACCCTATACTTTCAGCTTTGTGTCTAGCATGTCGGTGAATTTTAAATAACATCACATAGGTTAAAGACCTATTAGATATAAAATTAAAAATTCAGGAACTTATTTGACACAAAATAAAAAGTTTAAAGACCTACAAGACACCTTCTAAAGTTTAGAAGTCAAATAGACCCAAACATGAAAGTTTAGGGAATAAACTTGCCATTTAACCCATAAATAAAGGTAGTTTGAGGCTTCTAAAAAGTAATCAGTTTTTTTTTTCCCGATAAGAAACGATTTCATTCAAGGGATAAACAAAAGAGACCATAAAATGGGATTACAAGTAATCAGTTTTGCTTTGGATGGTAGACACAAATCCCAGCATTAATATTAATCCAATTGACTGAATTAATGAGATGGAACTCAACTTTTCTATGACATTCAATTTTCTTCATGTCAAACCAGCACAATAAACAATCTCATTAGCCCTCTCTCCAATTTGTTATTACTACTACATACAGATTTCTGAATCCTGTTTATTGAAAAGTAAATCATAACAGGATCTAAAAGGAATTATGTTGCTGTTATTTAGCAACTTTCTTTCTCTGCTCTTGTTTATTCGTTGAACTAAAAGCTGAGGTTTTGTGCATGCACATTTTATACAACTTGCTGCTTTTGATTGTTAATTCTTTAGGCTTTATTCATGCCTTATGACCATAAGAACACAACTCCTGACGGTGAAATTGCTCAACAAATGAAACTTCTGCTTGAAGAGCTGAACAATCTCCATTGCCACCACCGCTGTATGATTGGATCAGGCGGAGGTTCTGTCGGGTTAGTCCTTTTCTTCATGAACCCTTTTCTTTATTGTGATTCTGATTGTACTTTTTACAATCATATTCTAGTAGGGTACCATATTTCATATGTTTTATATGTATTTTATTGGCACTTTTTAGTTCAGAAATGTAGATTGAACCTTTTAAATACAGTTAAATATTACCAAGGTAGAGAATTCTTAGAACAACTAGAAGAAGTTCAGAAATGTAGATTGAACTTTTAAAGTTTAGAAAAAAAAATTAATTTATATTCTTAAACTTCGATGTATAAATTAAAACTCCAAATTAATAGTTATATCAATTTAGACTCTAAACTTTTATTTAAAATCAACTCAAAATCACCTATTCTTCTCCAAAAGATCTCAACAAAGGGTAGCATGCAATTCTTCCAAAAAATTAGAGAAAAACAAAAGTAGACGAGAAGATGAAAAACACTCCTATTAATGAAAAATCACATATTAATAGAGTCCATATCATCCTTAATGTGCAATTGGTTCTGAAATTGTTCCTGCATAATTATTGTTGTTAAGGTATCTGGCACATGGGACAGCCACAGATTTCATGTTTGACAAGGCACGAGTTCCTATGGCTTTCACATTTGAGGTTTGTCAAACAAGAACTGCTATGTAACTTGCATAAAATAATCTTGGTTTTGGCATTAAAATAAGCTTCTCTTCATCATATGATTATATCAGATATATGGAGACGAAGCTGCCTCATCAAAAGACTGCTTTAGAATGTTCAATCCCACAGATCCCGCTACCTTCAATGTATGTAACATCTCTTCTTGCCATATTGACCAGTTCATGGAAATCCTGGGCTGAATTCAATTTTCTTCTTGTTTTCAGAGGGTTCTCAGCGATTGGTCTGCTGCATTTTTTACAATATTTAAGCTGAGTCCCGAGTACCTTGGCGAGACCGAGCTCCATTCAAAGGGCAACTTAGACAAGTTGGTGTCGATTGATGAATATCTTGAAGGTTACTTGATTGAGAGGAGTAGCAGATATGGGAAGAAGAGGGAGGTGTTTGACCTAGGGATGCAAGAGATGAGAACTTATTTTAGGCTATTTTTGTTGTCATCAGTTTTATTGATGTTCATGTTTTGTTCTAGAATTTCTAAAAACAAGTTCACTAGACCACTTGTTTCTGCCATTTCCATTTGA

mRNA sequence

ATGAAGTCTAGAAATAAGGGCTATGTTGCAGAGATTCCAGTTGTTACTTATTGCCGTGGAAGGAATAACATTGATGACATGTCAAAATTCCGGATACTTCTTAGTTTTGGACAGCATGGAAGGGAGCTCATAACAACGGAAGTTGCTCTAAGAATTCTCTTAATCTTGAGTGAAGAACAGTTGTTGCCGCACATGGATCGAGCTTCCTTAAACAACACACTTGACAAGCTTGTCATTAAGGTGGTGCCAATGGAAAACTTGAATGGGCGCAGGCTTGTCGAGGGCGGGGATCTATGTGAAAGAAGAAATGGGAGAGGTGTTGATTTGAACAGAAATTGGAGTGTTGATTGGGGTAAAAAGGAAAAGGATTATGATCCTTATGAGGAAAATCCTGGATTTGCACCTTTCAGCGAACCAGAAACTCAAATTATGCGAAAACTTGCTTTAACATTTGATCCACATATATGGGTCAATGTGCACTCTGGAATGGAGGCTTTATTCATGCCTTATGACCATAAGAACACAACTCCTGACGGTGAAATTGCTCAACAAATGAAACTTCTGCTTGAAGAGCTGAACAATCTCCATTGCCACCACCGCTGTATGATTGGATCAGGCGGAGGTTCTGTCGGGTATCTGGCACATGGGACAGCCACAGATTTCATGTTTGACAAGGCACGAGTTCCTATGGCTTTCACATTTGAGATATATGGAGACGAAGCTGCCTCATCAAAAGACTGCTTTAGAATGTTCAATCCCACAGATCCCGCTACCTTCAATAGGGTTCTCAGCGATTGGTCTGCTGCATTTTTTACAATATTTAAGCTGAGTCCCGAGTACCTTGGCGAGACCGAGCTCCATTCAAAGGGCAACTTAGACAAGTTGGTGTCGATTGATGAATATCTTGAAGGTTACTTGATTGAGAGGAGTAGCAGATATGGGAAGAAGAGGGAGGTGTTTGACCTAGGGATGCAAGAGATGAGAACTTATTTTAGGCTATTTTTGTTGTCATCAGTTTTATTGATGTTCATGTTTTGTTCTAGAATTTCTAAAAACAAGTTCACTAGACCACTTGTTTCTGCCATTTCCATTTGA

Coding sequence (CDS)

ATGAAGTCTAGAAATAAGGGCTATGTTGCAGAGATTCCAGTTGTTACTTATTGCCGTGGAAGGAATAACATTGATGACATGTCAAAATTCCGGATACTTCTTAGTTTTGGACAGCATGGAAGGGAGCTCATAACAACGGAAGTTGCTCTAAGAATTCTCTTAATCTTGAGTGAAGAACAGTTGTTGCCGCACATGGATCGAGCTTCCTTAAACAACACACTTGACAAGCTTGTCATTAAGGTGGTGCCAATGGAAAACTTGAATGGGCGCAGGCTTGTCGAGGGCGGGGATCTATGTGAAAGAAGAAATGGGAGAGGTGTTGATTTGAACAGAAATTGGAGTGTTGATTGGGGTAAAAAGGAAAAGGATTATGATCCTTATGAGGAAAATCCTGGATTTGCACCTTTCAGCGAACCAGAAACTCAAATTATGCGAAAACTTGCTTTAACATTTGATCCACATATATGGGTCAATGTGCACTCTGGAATGGAGGCTTTATTCATGCCTTATGACCATAAGAACACAACTCCTGACGGTGAAATTGCTCAACAAATGAAACTTCTGCTTGAAGAGCTGAACAATCTCCATTGCCACCACCGCTGTATGATTGGATCAGGCGGAGGTTCTGTCGGGTATCTGGCACATGGGACAGCCACAGATTTCATGTTTGACAAGGCACGAGTTCCTATGGCTTTCACATTTGAGATATATGGAGACGAAGCTGCCTCATCAAAAGACTGCTTTAGAATGTTCAATCCCACAGATCCCGCTACCTTCAATAGGGTTCTCAGCGATTGGTCTGCTGCATTTTTTACAATATTTAAGCTGAGTCCCGAGTACCTTGGCGAGACCGAGCTCCATTCAAAGGGCAACTTAGACAAGTTGGTGTCGATTGATGAATATCTTGAAGGTTACTTGATTGAGAGGAGTAGCAGATATGGGAAGAAGAGGGAGGTGTTTGACCTAGGGATGCAAGAGATGAGAACTTATTTTAGGCTATTTTTGTTGTCATCAGTTTTATTGATGTTCATGTTTTGTTCTAGAATTTCTAAAAACAAGTTCACTAGACCACTTGTTTCTGCCATTTCCATTTGA

Protein sequence

MKSRNKGYVAEIPVVTYCRGRNNIDDMSKFRILLSFGQHGRELITTEVALRILLILSEEQLLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGEIAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDEAASSKDCFRMFNPTDPATFNRVLSDWSAAFFTIFKLSPEYLGETELHSKGNLDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVSAISI
Homology
BLAST of HG10004274 vs. NCBI nr
Match: XP_038883968.1 (metallocarboxypeptidase A-like protein MCYG_01475 [Benincasa hispida])

HSP 1 Score: 721.5 bits (1861), Expect = 3.7e-204
Identity = 351/364 (96.43%), Postives = 356/364 (97.80%), Query Frame = 0

Query: 1   MKSRNKGYVAEIPVVTYCRGRNNIDDMSKFRILLSFGQHGRELITTEVALRILLILSEEQ 60
           MKSRNKGYVAEIPVVTYC GRNNIDD SKFRILLSFGQHGRELITTEVALRIL ILSEEQ
Sbjct: 74  MKSRNKGYVAEIPVVTYCHGRNNIDDTSKFRILLSFGQHGRELITTEVALRILSILSEEQ 133

Query: 61  LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 120
           LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK
Sbjct: 134 LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 193

Query: 121 EKDYDPYEENPGFAPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 180
           EKDYDPYEENPGFAPFSEPETQIMR LALTFDPH+WVNVHSGMEALFMPYDHKNTTP+GE
Sbjct: 194 EKDYDPYEENPGFAPFSEPETQIMRNLALTFDPHMWVNVHSGMEALFMPYDHKNTTPEGE 253

Query: 181 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 240
           IAQQMKLL+EELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKA VPMAFTFEIYGDE
Sbjct: 254 IAQQMKLLVEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKAGVPMAFTFEIYGDE 313

Query: 241 AASSKDCFRMFNPTDPATFNRVLSDWSAAFFTIFKLSPEYLGETELHSKGNLDKLVSIDE 300
           AASSKDCFRMFNPTDPATFNRVL DWSAAFFTIFKL PEYLGET+LHSKGNLDKLVSIDE
Sbjct: 314 AASSKDCFRMFNPTDPATFNRVLGDWSAAFFTIFKLGPEYLGETKLHSKGNLDKLVSIDE 373

Query: 301 YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 360
           YLEGYLIERSSRYGKKREV DLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS
Sbjct: 374 YLEGYLIERSSRYGKKREVIDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 433

Query: 361 AISI 365
           AIS+
Sbjct: 434 AISL 437

BLAST of HG10004274 vs. NCBI nr
Match: XP_008464857.1 (PREDICTED: LOW QUALITY PROTEIN: metallocarboxypeptidase A-like protein TRV_02598 [Cucumis melo])

HSP 1 Score: 709.9 bits (1831), Expect = 1.1e-200
Identity = 347/364 (95.33%), Postives = 352/364 (96.70%), Query Frame = 0

Query: 1   MKSRNKGYVAEIPVVTYCRGRNNIDDMSKFRILLSFGQHGRELITTEVALRILLILSEEQ 60
           MKS+NKGY AEIPVVTY RGRNNIDD SKFRILLSFGQHGRELITTEVALRILLILSEE 
Sbjct: 144 MKSKNKGYAAEIPVVTYRRGRNNIDDTSKFRILLSFGQHGRELITTEVALRILLILSEEH 203

Query: 61  LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 120
           LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK
Sbjct: 204 LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 263

Query: 121 EKDYDPYEENPGFAPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 180
           EKDYDPYEENPGFAPFSEPETQIMRK+AL FDPHIWVNVHSGMEALFMPYDHKNTTPDGE
Sbjct: 264 EKDYDPYEENPGFAPFSEPETQIMRKIALRFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 323

Query: 181 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 240
           IAQQMKLLLEELN+LHCH RCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE
Sbjct: 324 IAQQMKLLLEELNHLHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 383

Query: 241 AASSKDCFRMFNPTDPATFNRVLSDWSAAFFTIFKLSPEYLGETELHSKGNLDKLVSIDE 300
           AASSKDCFRMFNPTDP TFNRVLSDWSAAFFTIFK+ PEYL ETE H K N+DKLVSIDE
Sbjct: 384 AASSKDCFRMFNPTDPNTFNRVLSDWSAAFFTIFKMGPEYLDETEFHPKSNVDKLVSIDE 443

Query: 301 YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 360
           YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS
Sbjct: 444 YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 503

Query: 361 AISI 365
           AISI
Sbjct: 504 AISI 507

BLAST of HG10004274 vs. NCBI nr
Match: KAA0038457.1 (metallocarboxypeptidase A-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 708.0 bits (1826), Expect = 4.3e-200
Identity = 346/364 (95.05%), Postives = 351/364 (96.43%), Query Frame = 0

Query: 1   MKSRNKGYVAEIPVVTYCRGRNNIDDMSKFRILLSFGQHGRELITTEVALRILLILSEEQ 60
           MKS+NKGY AEIPVVTY RGRNNIDD SKFRILLSFGQHGRELITTEVALRILLILSEE 
Sbjct: 47  MKSKNKGYAAEIPVVTYRRGRNNIDDTSKFRILLSFGQHGRELITTEVALRILLILSEEH 106

Query: 61  LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 120
           LLPHMD ASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK
Sbjct: 107 LLPHMDHASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 166

Query: 121 EKDYDPYEENPGFAPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 180
           EKDYDPYEENPGFAPFSEPETQIMRK+AL FDPHIWVNVHSGMEALFMPYDHKNTTPDGE
Sbjct: 167 EKDYDPYEENPGFAPFSEPETQIMRKIALRFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 226

Query: 181 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 240
           IAQQMKLLLEELN+LHCH RCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE
Sbjct: 227 IAQQMKLLLEELNHLHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 286

Query: 241 AASSKDCFRMFNPTDPATFNRVLSDWSAAFFTIFKLSPEYLGETELHSKGNLDKLVSIDE 300
           AASSKDCFRMFNPTDP TFNRVLSDWSAAFFTIFK+ PEYL ETE H K N+DKLVSIDE
Sbjct: 287 AASSKDCFRMFNPTDPNTFNRVLSDWSAAFFTIFKMGPEYLDETEFHPKSNVDKLVSIDE 346

Query: 301 YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 360
           YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS
Sbjct: 347 YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 406

Query: 361 AISI 365
           AISI
Sbjct: 407 AISI 410

BLAST of HG10004274 vs. NCBI nr
Match: XP_023537607.1 (metallocarboxypeptidase A-like protein MCYG_01475 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 702.6 bits (1812), Expect = 1.8e-198
Identity = 340/363 (93.66%), Postives = 351/363 (96.69%), Query Frame = 0

Query: 1   MKSRNKGYVAEIPVVTYCRGRNNIDDMSKFRILLSFGQHGRELITTEVALRILLILSEEQ 60
           MKSRNKGYV E+PVVTY  GRNNIDD+SKFRILLSFGQHGRELITTE+ALRILLILSEE 
Sbjct: 77  MKSRNKGYVVEMPVVTYRHGRNNIDDISKFRILLSFGQHGRELITTELALRILLILSEEL 136

Query: 61  LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 120
            LPHMDRASLNNTL+KLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK
Sbjct: 137 FLPHMDRASLNNTLNKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 196

Query: 121 EKDYDPYEENPGFAPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 180
           EKDYDPYEENPG +PFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE
Sbjct: 197 EKDYDPYEENPGLSPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 256

Query: 181 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 240
           IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPM+FTFEIYGD 
Sbjct: 257 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMSFTFEIYGDA 316

Query: 241 AASSKDCFRMFNPTDPATFNRVLSDWSAAFFTIFKLSPEYLGETELHSKGNLDKLVSIDE 300
           AASS DCF+MFNPTDPATFNRVL DWSAAFFTIFKL PEYLGE +LH KGNLDKLVSIDE
Sbjct: 317 AASSNDCFKMFNPTDPATFNRVLGDWSAAFFTIFKLGPEYLGEIKLHRKGNLDKLVSIDE 376

Query: 301 YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 360
           YLEGYLIERSSRYGKKREVFDLG+QEMRTYFRLFLLSSVLLMFMFCSRISKNKFT+PLVS
Sbjct: 377 YLEGYLIERSSRYGKKREVFDLGLQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTKPLVS 436

Query: 361 AIS 364
           A+S
Sbjct: 437 ALS 439

BLAST of HG10004274 vs. NCBI nr
Match: KAG6585782.1 (Carboxypeptidase A6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 701.0 bits (1808), Expect = 5.2e-198
Identity = 339/363 (93.39%), Postives = 351/363 (96.69%), Query Frame = 0

Query: 1   MKSRNKGYVAEIPVVTYCRGRNNIDDMSKFRILLSFGQHGRELITTEVALRILLILSEEQ 60
           MKSRNKGYV E+PVVTY  GRNNIDD+SKFRILLSFGQHGRELITTE+ALRILLILSEE 
Sbjct: 79  MKSRNKGYVVEMPVVTYRHGRNNIDDISKFRILLSFGQHGRELITTELALRILLILSEEL 138

Query: 61  LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 120
            LPHMDRASLNNTL+KLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK
Sbjct: 139 FLPHMDRASLNNTLNKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 198

Query: 121 EKDYDPYEENPGFAPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 180
           EKDYDPYEENPG +PFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE
Sbjct: 199 EKDYDPYEENPGLSPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 258

Query: 181 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 240
           IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPM+FTFEIYGD 
Sbjct: 259 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMSFTFEIYGDA 318

Query: 241 AASSKDCFRMFNPTDPATFNRVLSDWSAAFFTIFKLSPEYLGETELHSKGNLDKLVSIDE 300
           AASS DCF+MFNPTDPATFNRVL DWSAAFFTIFKL PEYLGE +LH KGNLDKLVSIDE
Sbjct: 319 AASSNDCFKMFNPTDPATFNRVLGDWSAAFFTIFKLGPEYLGEIKLHRKGNLDKLVSIDE 378

Query: 301 YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 360
           YLEGYLIERSSRYGKKREVF+LG+QEMRTYFRLFLLSSVLLMFMFCSRISKNKFT+PLVS
Sbjct: 379 YLEGYLIERSSRYGKKREVFELGLQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTKPLVS 438

Query: 361 AIS 364
           A+S
Sbjct: 439 ALS 441

BLAST of HG10004274 vs. ExPASy Swiss-Prot
Match: Q8N4T0 (Carboxypeptidase A6 OS=Homo sapiens OX=9606 GN=CPA6 PE=1 SV=2)

HSP 1 Score: 55.5 bits (132), Expect = 1.5e-06
Identity = 57/221 (25.79%), Postives = 94/221 (42.53%), Query Frame = 0

Query: 29  KFRILLSFGQHGRELITTEVALRILLILSEEQLLPHMDRASLNNTLDKLVIKVVPMENLN 88
           K  + +  G H RE I        +    +E LL +    ++   L+ L   ++P+ N++
Sbjct: 186 KRAVWIDCGIHAREWIGPAFCQWFV----KEALLTYKSDPAMRKMLNHLYFYIMPVFNVD 245

Query: 89  GRRLVEGGDLCER----RNG----RGVDLNRNWSVDWGKKEKDYDPYEEN-PGFAPFSEP 148
           G       D   R    RN     RGVD NRNW V W  +     P ++   G  P SEP
Sbjct: 246 GYHFSWTNDRFWRKTRSRNSRFRCRGVDANRNWKVKWCDEGASMHPCDDTYCGPFPESEP 305

Query: 149 ETQIMRKLALTFDPHI--WVNVHSGMEALFMPYDHKNTT-PDGEIAQQMKLLLEELNNLH 208
           E + +         HI  +++ H+  + L  PY +K  T P+    +      + +N L 
Sbjct: 306 EVKAVANFLRKHRKHIRAYLSFHAYAQMLLYPYSYKYATIPNFRCVE--SAAYKAVNALQ 365

Query: 209 CHHRCMIGSGGGSVG-YLAHGTATDFMFDKARVPMAFTFEI 237
             +      G  S   Y++ G++ D+ + K  +P AF FE+
Sbjct: 366 SVYGVRYRYGPASTTLYVSSGSSMDWAY-KNGIPYAFAFEL 399

BLAST of HG10004274 vs. ExPASy Swiss-Prot
Match: Q5B011 (Putative metallocarboxypeptidase ecm14 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=ecm14 PE=3 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 9.7e-06
Identity = 66/277 (23.83%), Postives = 110/277 (39.71%), Query Frame = 0

Query: 20  GRNNIDDMSKFRILLSFGQHGRELITTEVALRILLILSEEQLLPHMDRASLNNTLDKLVI 79
           GR++    S+  IL+  G H RE I+T     I   L    +  +   A++ + L     
Sbjct: 233 GRSSQPGESRPMILIVGGNHAREWISTSTVAYIAYNL----MTRYGSSAAVTSLLQDYDW 292

Query: 80  KVVPMENLNGRRLVEGGDLCERRNGR--------GVDLNRNWSVDWGKKEKDYDPYEEN- 139
            +VP  N +G       D   R+N +        G+DL+R+WS +W   +   +P  EN 
Sbjct: 293 VLVPTVNPDGYVYTWDSDRLWRKNRQPTSFRFCPGIDLDRSWSFEWDGNQTQTNPCSENY 352

Query: 140 PGFAPFSEPETQIMRKLALTFDPHI------WVNVHSGMEALFMPYDH--KNTTPDGEIA 199
            G  PF   E Q + + AL    H       +++ HS  + +  PY +   +  P  E  
Sbjct: 353 AGEEPFEGVEAQQLAEWALNETEHNNARFVGFLDFHSYAQQILYPYTYSCSSVPPTLESL 412

Query: 200 QQMKLLLEELNNLHCHH------RC----MIGSGGGS------VGYLAHGTATDFMFDKA 259
           +++ L L ++  L  H        C    +  SG  S      VG  + G+  D+ + + 
Sbjct: 413 EELGLGLAKVIRLTTHEIYDVTSACEGVVIPASGDKSAKSLFPVGGSSGGSGLDWFYHQL 472

Query: 260 RVPMAFTFEIYGDEAASSKDCFRMFNPTDPATFNRVL 264
           R   A+  ++    +           PT    FN VL
Sbjct: 473 RTRYAYQIKLRDRGSYGFLLPSEYIVPTGTEAFNAVL 505

BLAST of HG10004274 vs. ExPASy Swiss-Prot
Match: Q5U901 (Carboxypeptidase A6 OS=Mus musculus OX=10090 GN=Cpa6 PE=2 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.8e-04
Identity = 52/224 (23.21%), Postives = 89/224 (39.73%), Query Frame = 0

Query: 29  KFRILLSFGQHGRELITTEVALRILLILSEEQLLPHMDRASLNNTLDKLVIKVVPMENLN 88
           K  + +  G H RE I        +     E +L +    ++   L+ L   ++P+ N++
Sbjct: 187 KRAVWIDCGIHAREWIGPAFCQWFV----REAILTYKTDPAMKKMLNHLYFYIMPVFNVD 246

Query: 89  GRRLVEGGDLCERRNG--------RGVDLNRNWSVDWGKKEKDYDPYEEN-PGFAPFSEP 148
           G       D   R+          RGVD NRNW V W  +     P ++   G  P SEP
Sbjct: 247 GYHFSWTHDRFWRKTRSRDSKFRCRGVDANRNWKVKWCDEGASAHPCDDTYCGPFPESEP 306

Query: 149 ETQIMRKLALTFDPHI--WVNVHSGMEALFMPYDHKNTT-PDGE----IAQQMKLLLEEL 208
           E + +          I  +++ H+  + L  PY +K  T P+       A +    L  +
Sbjct: 307 EVKAVANFLRKHRKRIRAYLSFHAYAQMLLYPYSYKYATIPNFSCVEFAAHKAVKALRSV 366

Query: 209 NNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEI 237
           + +   H            Y++ G + D+ + K  +P AF FE+
Sbjct: 367 HGIRYRH-----GPASQTLYVSSGNSMDWAY-KNGIPYAFAFEL 400

BLAST of HG10004274 vs. ExPASy TrEMBL
Match: A0A1S3CMF4 (LOW QUALITY PROTEIN: metallocarboxypeptidase A-like protein TRV_02598 OS=Cucumis melo OX=3656 GN=LOC103502627 PE=3 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 5.4e-201
Identity = 347/364 (95.33%), Postives = 352/364 (96.70%), Query Frame = 0

Query: 1   MKSRNKGYVAEIPVVTYCRGRNNIDDMSKFRILLSFGQHGRELITTEVALRILLILSEEQ 60
           MKS+NKGY AEIPVVTY RGRNNIDD SKFRILLSFGQHGRELITTEVALRILLILSEE 
Sbjct: 144 MKSKNKGYAAEIPVVTYRRGRNNIDDTSKFRILLSFGQHGRELITTEVALRILLILSEEH 203

Query: 61  LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 120
           LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK
Sbjct: 204 LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 263

Query: 121 EKDYDPYEENPGFAPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 180
           EKDYDPYEENPGFAPFSEPETQIMRK+AL FDPHIWVNVHSGMEALFMPYDHKNTTPDGE
Sbjct: 264 EKDYDPYEENPGFAPFSEPETQIMRKIALRFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 323

Query: 181 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 240
           IAQQMKLLLEELN+LHCH RCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE
Sbjct: 324 IAQQMKLLLEELNHLHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 383

Query: 241 AASSKDCFRMFNPTDPATFNRVLSDWSAAFFTIFKLSPEYLGETELHSKGNLDKLVSIDE 300
           AASSKDCFRMFNPTDP TFNRVLSDWSAAFFTIFK+ PEYL ETE H K N+DKLVSIDE
Sbjct: 384 AASSKDCFRMFNPTDPNTFNRVLSDWSAAFFTIFKMGPEYLDETEFHPKSNVDKLVSIDE 443

Query: 301 YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 360
           YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS
Sbjct: 444 YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 503

Query: 361 AISI 365
           AISI
Sbjct: 504 AISI 507

BLAST of HG10004274 vs. ExPASy TrEMBL
Match: A0A5A7TA59 (Metallocarboxypeptidase A-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold119G00240 PE=3 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 2.1e-200
Identity = 346/364 (95.05%), Postives = 351/364 (96.43%), Query Frame = 0

Query: 1   MKSRNKGYVAEIPVVTYCRGRNNIDDMSKFRILLSFGQHGRELITTEVALRILLILSEEQ 60
           MKS+NKGY AEIPVVTY RGRNNIDD SKFRILLSFGQHGRELITTEVALRILLILSEE 
Sbjct: 47  MKSKNKGYAAEIPVVTYRRGRNNIDDTSKFRILLSFGQHGRELITTEVALRILLILSEEH 106

Query: 61  LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 120
           LLPHMD ASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK
Sbjct: 107 LLPHMDHASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 166

Query: 121 EKDYDPYEENPGFAPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 180
           EKDYDPYEENPGFAPFSEPETQIMRK+AL FDPHIWVNVHSGMEALFMPYDHKNTTPDGE
Sbjct: 167 EKDYDPYEENPGFAPFSEPETQIMRKIALRFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 226

Query: 181 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 240
           IAQQMKLLLEELN+LHCH RCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE
Sbjct: 227 IAQQMKLLLEELNHLHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 286

Query: 241 AASSKDCFRMFNPTDPATFNRVLSDWSAAFFTIFKLSPEYLGETELHSKGNLDKLVSIDE 300
           AASSKDCFRMFNPTDP TFNRVLSDWSAAFFTIFK+ PEYL ETE H K N+DKLVSIDE
Sbjct: 287 AASSKDCFRMFNPTDPNTFNRVLSDWSAAFFTIFKMGPEYLDETEFHPKSNVDKLVSIDE 346

Query: 301 YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 360
           YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS
Sbjct: 347 YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 406

Query: 361 AISI 365
           AISI
Sbjct: 407 AISI 410

BLAST of HG10004274 vs. ExPASy TrEMBL
Match: A0A6J1KIA8 (metallocarboxypeptidase A-like protein MCYG_01475 OS=Cucurbita maxima OX=3661 GN=LOC111496034 PE=3 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 9.6e-198
Identity = 338/364 (92.86%), Postives = 351/364 (96.43%), Query Frame = 0

Query: 1   MKSRNKGYVAEIPVVTYCRGRNNIDDMSKFRILLSFGQHGRELITTEVALRILLILSEEQ 60
           MKSRNKGYV E+PVVTY  GRNNIDD+SKFRILLSFGQHGRELITTE+ALRILLILSEE 
Sbjct: 73  MKSRNKGYVVEMPVVTYRHGRNNIDDISKFRILLSFGQHGRELITTELALRILLILSEEL 132

Query: 61  LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 120
            LPHMDRASLNNTL KLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK
Sbjct: 133 FLPHMDRASLNNTLTKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 192

Query: 121 EKDYDPYEENPGFAPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 180
           EKDYDPYEENPG +PFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE
Sbjct: 193 EKDYDPYEENPGLSPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 252

Query: 181 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 240
           IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPM+FTFEIYGD 
Sbjct: 253 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMSFTFEIYGDA 312

Query: 241 AASSKDCFRMFNPTDPATFNRVLSDWSAAFFTIFKLSPEYLGETELHSKGNLDKLVSIDE 300
           AASS DCF+MFNPTDP TFNRVL DWSAAFFTIFKL PEYLGET+LH KGNLDKLVSIDE
Sbjct: 313 AASSNDCFKMFNPTDPDTFNRVLGDWSAAFFTIFKLGPEYLGETKLHRKGNLDKLVSIDE 372

Query: 301 YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 360
           YLEGYLIERSSRYGKKREVF+LG+QEMRTYFRLFLLSSVLLMFMFCSRISK+KFT+PLVS
Sbjct: 373 YLEGYLIERSSRYGKKREVFELGLQEMRTYFRLFLLSSVLLMFMFCSRISKSKFTKPLVS 432

Query: 361 AISI 365
           A+S+
Sbjct: 433 ALSL 436

BLAST of HG10004274 vs. ExPASy TrEMBL
Match: A0A6J1GHD9 (metallocarboxypeptidase A-like protein TRV_02598 OS=Cucurbita moschata OX=3662 GN=LOC111454214 PE=3 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 1.6e-197
Identity = 338/363 (93.11%), Postives = 350/363 (96.42%), Query Frame = 0

Query: 1   MKSRNKGYVAEIPVVTYCRGRNNIDDMSKFRILLSFGQHGRELITTEVALRILLILSEEQ 60
           MKSRNKGYV E+PVVTY  GRNNIDD+SKFRILLSFGQHGRELITTE+ALRILLILSEE 
Sbjct: 76  MKSRNKGYVVEMPVVTYRHGRNNIDDISKFRILLSFGQHGRELITTELALRILLILSEEL 135

Query: 61  LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 120
            LPHMDRASLNNTL+KLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK
Sbjct: 136 FLPHMDRASLNNTLNKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 195

Query: 121 EKDYDPYEENPGFAPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 180
           EKDYDPYEENPG +PFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE
Sbjct: 196 EKDYDPYEENPGLSPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 255

Query: 181 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 240
           IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPM+FTFEIYGD 
Sbjct: 256 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMSFTFEIYGDA 315

Query: 241 AASSKDCFRMFNPTDPATFNRVLSDWSAAFFTIFKLSPEYLGETELHSKGNLDKLVSIDE 300
           AASS DCF+MFNPTDPATFNRVL DWSAAFFTIFKL P YLGE +LH KGNLDKLVSIDE
Sbjct: 316 AASSNDCFKMFNPTDPATFNRVLGDWSAAFFTIFKLGPGYLGEIKLHRKGNLDKLVSIDE 375

Query: 301 YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 360
           YLEGYLIERSSRYGKKREVF+LG+QEMRTYFRLFLLSSVLLMFMFCSRISKNKFT+PLVS
Sbjct: 376 YLEGYLIERSSRYGKKREVFELGLQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTKPLVS 435

Query: 361 AIS 364
           A+S
Sbjct: 436 ALS 438

BLAST of HG10004274 vs. ExPASy TrEMBL
Match: A0A6J1DJS2 (carboxypeptidase A6 OS=Momordica charantia OX=3673 GN=LOC111020704 PE=3 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 1.4e-196
Identity = 336/364 (92.31%), Postives = 350/364 (96.15%), Query Frame = 0

Query: 1   MKSRNKGYVAEIPVVTYCRGRNNIDDMSKFRILLSFGQHGRELITTEVALRILLILSEEQ 60
           M+S NKGY+AEIPVVTYC GR NIDD+SKFRILLSFGQHGRELITTE+ALRILL+LS EQ
Sbjct: 76  MRSGNKGYLAEIPVVTYCHGRKNIDDLSKFRILLSFGQHGRELITTELALRILLLLSGEQ 135

Query: 61  LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 120
            LPHMD+ASLNNTL  LVIKVVPMENLNGRRLVE GDLCERRNGRGVDLNRNWSVDWGKK
Sbjct: 136 FLPHMDQASLNNTLANLVIKVVPMENLNGRRLVEAGDLCERRNGRGVDLNRNWSVDWGKK 195

Query: 121 EKDYDPYEENPGFAPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 180
           EKDYDPYEENPGFAPFSEPETQ+MRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDG+
Sbjct: 196 EKDYDPYEENPGFAPFSEPETQVMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGD 255

Query: 181 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 240
           IAQQMKL+LEELNNLHCH RCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGD 
Sbjct: 256 IAQQMKLVLEELNNLHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDG 315

Query: 241 AASSKDCFRMFNPTDPATFNRVLSDWSAAFFTIFKLSPEYLGETELHSKGNLDKLVSIDE 300
           AASSKDCF+MFNPTD  TFNRVLSDWSAAFFTIFKL PEYLG+TELHSKGNLDKLVSIDE
Sbjct: 316 AASSKDCFKMFNPTDLGTFNRVLSDWSAAFFTIFKLGPEYLGDTELHSKGNLDKLVSIDE 375

Query: 301 YLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVS 360
           YLEGYLIERSSRYGKKREV +LGMQEMRTYFRLFLLSS+LL+FMFCSRISKNKFTRPLVS
Sbjct: 376 YLEGYLIERSSRYGKKREVLELGMQEMRTYFRLFLLSSILLLFMFCSRISKNKFTRPLVS 435

Query: 361 AISI 365
           AISI
Sbjct: 436 AISI 439

BLAST of HG10004274 vs. TAIR 10
Match: AT5G42320.1 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 542.7 bits (1397), Expect = 2.2e-154
Identity = 262/360 (72.78%), Postives = 299/360 (83.06%), Query Frame = 0

Query: 1   MKSRNKGYVAEIPVVTYCRGRNNIDDMSKFRILLSFGQHGRELITTEVALRILLILSEEQ 60
           +KS NKGY AE+ VVTYCRG    DD S FRILL+FGQHGRELIT+E+A RIL ILSEEQ
Sbjct: 20  IKSGNKGYNAEVNVVTYCRGGKESDDRSNFRILLTFGQHGRELITSELAFRILSILSEEQ 79

Query: 61  LLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKK 120
            LP+ +   L NTLDKLVIK+VP+EN NGR+ VE GDLCERRNGRGVDLNRNW VDWGKK
Sbjct: 80  FLPNKNGGILKNTLDKLVIKMVPIENPNGRKRVESGDLCERRNGRGVDLNRNWGVDWGKK 139

Query: 121 EKDYDPYEENPGFAPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFMPYDHKNTTPDGE 180
           EKDYDP EENPG APFSEPETQIMRKLA++FDPHIW+NVHSGMEALFMPYDHKN TP+G 
Sbjct: 140 EKDYDPSEENPGTAPFSEPETQIMRKLAISFDPHIWINVHSGMEALFMPYDHKNITPEGL 199

Query: 181 IAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDE 240
            +Q+M+ LLE+LN  HCH RCMIGSGGGSVGYLAHGTATD+++D  + PMAFTFEIYGD 
Sbjct: 200 PSQKMRTLLEKLNKFHCHDRCMIGSGGGSVGYLAHGTATDYIYDVVKAPMAFTFEIYGDN 259

Query: 241 AASSKDCFRMFNPTDPATFNRVLSDWSAAFFTIFKLSPEYLGETELHSKGN----LDKLV 300
             +S+DCF+MFNP D   F RVL+DWSAAFFTIF+L P       LH  GN     DK V
Sbjct: 260 QTASRDCFKMFNPVDLPNFKRVLNDWSAAFFTIFQLGP-------LHLDGNTSKAADKWV 319

Query: 301 SIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTR 357
           SIDEYL+GYL+ER +RYGK  EV D+GMQE++TYFRLFLLSSVLLMFMFCSRI+K+K++R
Sbjct: 320 SIDEYLDGYLVERKNRYGKNMEVIDVGMQEIKTYFRLFLLSSVLLMFMFCSRIAKSKYSR 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038883968.13.7e-20496.43metallocarboxypeptidase A-like protein MCYG_01475 [Benincasa hispida][more]
XP_008464857.11.1e-20095.33PREDICTED: LOW QUALITY PROTEIN: metallocarboxypeptidase A-like protein TRV_02598... [more]
KAA0038457.14.3e-20095.05metallocarboxypeptidase A-like protein [Cucumis melo var. makuwa][more]
XP_023537607.11.8e-19893.66metallocarboxypeptidase A-like protein MCYG_01475 [Cucurbita pepo subsp. pepo][more]
KAG6585782.15.2e-19893.39Carboxypeptidase A6, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q8N4T01.5e-0625.79Carboxypeptidase A6 OS=Homo sapiens OX=9606 GN=CPA6 PE=1 SV=2[more]
Q5B0119.7e-0623.83Putative metallocarboxypeptidase ecm14 OS=Emericella nidulans (strain FGSC A4 / ... [more]
Q5U9011.8e-0423.21Carboxypeptidase A6 OS=Mus musculus OX=10090 GN=Cpa6 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CMF45.4e-20195.33LOW QUALITY PROTEIN: metallocarboxypeptidase A-like protein TRV_02598 OS=Cucumis... [more]
A0A5A7TA592.1e-20095.05Metallocarboxypeptidase A-like protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1KIA89.6e-19892.86metallocarboxypeptidase A-like protein MCYG_01475 OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1GHD91.6e-19793.11metallocarboxypeptidase A-like protein TRV_02598 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1DJS21.4e-19692.31carboxypeptidase A6 OS=Momordica charantia OX=3673 GN=LOC111020704 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G42320.12.2e-15472.78Zn-dependent exopeptidases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000834Peptidase M14, carboxypeptidase ASMARTSM00631zn_carbcoord: 1..246
e-value: 1.2E-21
score: 88.0
IPR000834Peptidase M14, carboxypeptidase APFAMPF00246Peptidase_M14coord: 31..249
e-value: 7.9E-25
score: 88.2
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 9..261
e-value: 1.7E-45
score: 157.6
NoneNo IPR availablePANTHERPTHR11705:SF119OS02G0119300 PROTEINcoord: 2..363
NoneNo IPR availablePANTHERPTHR11705PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,Bcoord: 2..363
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 12..275
IPR034269At5g42320-like, carboxypeptidase domainCDDcd06227M14-CPA-likecoord: 29..251
e-value: 1.23154E-105
score: 307.661

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10004274.1HG10004274.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004181 metallocarboxypeptidase activity
molecular_function GO:0008270 zinc ion binding