HG10004027 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10004027
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionVacuolar iron transporter family protein
LocationChr08: 12945122 .. 12945775 (+)
RNA-Seq ExpressionHG10004027
SyntenyHG10004027
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCCCAACAAATTAAACCTAAGTACTTTGGAGCAACAATCCAATTTGGAAATCCAAACAAAAGAATTTGACTACTCGAAACGCGCACAATGGCTACGTGCCGCCGTGCTCGGTGCCAACGACGGGCTCGTCTCAACTGCGTCGCTTATGATGGGAGTTGGAGCTGTGAAACAAGACATCAAGGCCATGATCCTCACCGGTTTCGCCGGGTTAGTCGCCGGGGCGTGTAGTATGGCAATCGGTGAGTTTGTGTCTGTTTACTCTCAATTAGACATTGAAATGGCTCAAATAAAAAGAGAGAAATTACAAAGAAGTAGTAATATGGAAGGAGGAGGAGTAGTACAAGGACAAGAGAGCAAAGATGAAGAAAAACTACCAAATCCACTCCAAGCAGCTGCAGCCTCTGCCTTGGCATTCTCTCTAGGAGCAATGGTTCCACTATTGGCAGCTTCCTTTATAAGAGAATATAAAGTAAGGCTCGCGGTGGTCGTGGCAGCGGTTACGTTAGCTCTCGTCGTGTTTGGGTGGTTGGGTGCTATTCTTGGAAAAGCATCACCGATCAAATCAGCTTCTAGAGTGTTGGTCGGAGGTTGGTTAGCTATGGCTATAACCTTTGGCTTGACAAAGCTAATCGGAGCAAGCGGACTCTAA

mRNA sequence

ATGGATCCCAACAAATTAAACCTAAGTACTTTGGAGCAACAATCCAATTTGGAAATCCAAACAAAAGAATTTGACTACTCGAAACGCGCACAATGGCTACGTGCCGCCGTGCTCGGTGCCAACGACGGGCTCGTCTCAACTGCGTCGCTTATGATGGGAGTTGGAGCTGTGAAACAAGACATCAAGGCCATGATCCTCACCGGTTTCGCCGGGTTAGTCGCCGGGGCGTGTAGTATGGCAATCGGTGAGTTTGTGTCTGTTTACTCTCAATTAGACATTGAAATGGCTCAAATAAAAAGAGAGAAATTACAAAGAAGTAGTAATATGGAAGGAGGAGGAGTAGTACAAGGACAAGAGAGCAAAGATGAAGAAAAACTACCAAATCCACTCCAAGCAGCTGCAGCCTCTGCCTTGGCATTCTCTCTAGGAGCAATGGTTCCACTATTGGCAGCTTCCTTTATAAGAGAATATAAAGTAAGGCTCGCGGTGGTCGTGGCAGCGGTTACGTTAGCTCTCGTCGTGTTTGGGTGGTTGGGTGCTATTCTTGGAAAAGCATCACCGATCAAATCAGCTTCTAGAGTGTTGGTCGGAGGTTGGTTAGCTATGGCTATAACCTTTGGCTTGACAAAGCTAATCGGAGCAAGCGGACTCTAA

Coding sequence (CDS)

ATGGATCCCAACAAATTAAACCTAAGTACTTTGGAGCAACAATCCAATTTGGAAATCCAAACAAAAGAATTTGACTACTCGAAACGCGCACAATGGCTACGTGCCGCCGTGCTCGGTGCCAACGACGGGCTCGTCTCAACTGCGTCGCTTATGATGGGAGTTGGAGCTGTGAAACAAGACATCAAGGCCATGATCCTCACCGGTTTCGCCGGGTTAGTCGCCGGGGCGTGTAGTATGGCAATCGGTGAGTTTGTGTCTGTTTACTCTCAATTAGACATTGAAATGGCTCAAATAAAAAGAGAGAAATTACAAAGAAGTAGTAATATGGAAGGAGGAGGAGTAGTACAAGGACAAGAGAGCAAAGATGAAGAAAAACTACCAAATCCACTCCAAGCAGCTGCAGCCTCTGCCTTGGCATTCTCTCTAGGAGCAATGGTTCCACTATTGGCAGCTTCCTTTATAAGAGAATATAAAGTAAGGCTCGCGGTGGTCGTGGCAGCGGTTACGTTAGCTCTCGTCGTGTTTGGGTGGTTGGGTGCTATTCTTGGAAAAGCATCACCGATCAAATCAGCTTCTAGAGTGTTGGTCGGAGGTTGGTTAGCTATGGCTATAACCTTTGGCTTGACAAAGCTAATCGGAGCAAGCGGACTCTAA

Protein sequence

MDPNKLNLSTLEQQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQDIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQESKDEEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGAILGKASPIKSASRVLVGGWLAMAITFGLTKLIGASGL
Homology
BLAST of HG10004027 vs. NCBI nr
Match: XP_008441522.1 (PREDICTED: vacuolar iron transporter homolog 4-like [Cucumis melo] >KAA0054933.1 vacuolar iron transporter-like protein 4-like [Cucumis melo var. makuwa] >TYK22722.1 vacuolar iron transporter-like protein 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 370.2 bits (949), Expect = 1.3e-98
Identity = 207/218 (94.95%), Postives = 213/218 (97.71%), Query Frame = 0

Query: 1   MDPNKLNLSTLE-QQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ 60
           MDPNKLNL+ LE QQS LEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ
Sbjct: 1   MDPNKLNLNNLEQQQSTLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ 60

Query: 61  DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQE 120
           DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRS+NME GGVVQGQE
Sbjct: 61  DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSNNME-GGVVQGQE 120

Query: 121 SKDEEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLG 180
           SKD+EKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVA+VTLAL VFGWLG
Sbjct: 121 SKDKEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVASVTLALAVFGWLG 180

Query: 181 AILGKASPIKSASRVLVGGWLAMAITFGLTKLIGASGL 218
           A+LGKASPIKSA+RVLVGGWLAMAITFGLTKLIGASGL
Sbjct: 181 AVLGKASPIKSAARVLVGGWLAMAITFGLTKLIGASGL 217

BLAST of HG10004027 vs. NCBI nr
Match: XP_038885661.1 (vacuolar iron transporter homolog 4-like [Benincasa hispida])

HSP 1 Score: 367.5 bits (942), Expect = 8.1e-98
Identity = 208/218 (95.41%), Postives = 212/218 (97.25%), Query Frame = 0

Query: 1   MDPNKLNLSTLEQQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQD 60
           M+PNKLNLS LEQQS LEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQD
Sbjct: 1   MNPNKLNLSNLEQQSTLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQD 60

Query: 61  IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQE- 120
           IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNME G VVQGQE 
Sbjct: 61  IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNME-GVVVQGQEI 120

Query: 121 SKDEEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLG 180
           SKDEEKLPNPLQAAAASALAFSLGA+VPLLAASFIREYKVRLAVVVAAVTLALVVFGWLG
Sbjct: 121 SKDEEKLPNPLQAAAASALAFSLGAIVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLG 180

Query: 181 AILGKASPIKSASRVLVGGWLAMAITFGLTKLIGASGL 218
           A+LGKASPIKSA+RVLVGGWLAMAITFGLTKLI ASGL
Sbjct: 181 AVLGKASPIKSATRVLVGGWLAMAITFGLTKLIAASGL 217

BLAST of HG10004027 vs. NCBI nr
Match: XP_004140970.1 (LOW QUALITY PROTEIN: vacuolar iron transporter homolog 4 [Cucumis sativus] >XP_031737492.1 LOW QUALITY PROTEIN: vacuolar iron transporter homolog 4-like [Cucumis sativus])

HSP 1 Score: 367.1 bits (941), Expect = 1.1e-97
Identity = 205/218 (94.04%), Postives = 212/218 (97.25%), Query Frame = 0

Query: 1   MDPNKLNLSTLE-QQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ 60
           MDPNKLNL+ LE QQS LEIQ KEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ
Sbjct: 1   MDPNKLNLNNLEQQQSTLEIQAKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ 60

Query: 61  DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQE 120
           DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRS+NME GGVVQGQ+
Sbjct: 61  DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSNNME-GGVVQGQD 120

Query: 121 SKDEEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLG 180
           SKD+EKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVA+VTLAL VFGWLG
Sbjct: 121 SKDKEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVASVTLALAVFGWLG 180

Query: 181 AILGKASPIKSASRVLVGGWLAMAITFGLTKLIGASGL 218
           AILGKASPIKSA+RVL+GGWLAMAITFGLTKLIGASGL
Sbjct: 181 AILGKASPIKSAARVLIGGWLAMAITFGLTKLIGASGL 217

BLAST of HG10004027 vs. NCBI nr
Match: XP_022993928.1 (vacuolar iron transporter homolog 4-like [Cucurbita maxima])

HSP 1 Score: 344.0 bits (881), Expect = 9.6e-91
Identity = 195/217 (89.86%), Postives = 201/217 (92.63%), Query Frame = 0

Query: 1   MDPNKLNLSTLEQQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQD 60
           M+PNKLNL+ LEQQS  EIQT EFDYSKRAQWLRAAVLGANDGLVSTASLMMG+GAVKQD
Sbjct: 15  MEPNKLNLTNLEQQSGSEIQTNEFDYSKRAQWLRAAVLGANDGLVSTASLMMGIGAVKQD 74

Query: 61  IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQES 120
           IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIE+AQIKREKLQR  N+EG    Q QES
Sbjct: 75  IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEVAQIKREKLQR-RNLEG----QEQES 134

Query: 121 KDEEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGA 180
           KD+EKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRL VVVAAVTLAL VFGWLGA
Sbjct: 135 KDDEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLVVVVAAVTLALGVFGWLGA 194

Query: 181 ILGKASPIKSASRVLVGGWLAMAITFGLTKLIGASGL 218
           ILGKASPIKS  RVLVGGWLAMAITFGLTKLIG SGL
Sbjct: 195 ILGKASPIKSTIRVLVGGWLAMAITFGLTKLIGVSGL 226

BLAST of HG10004027 vs. NCBI nr
Match: XP_023551056.1 (vacuolar iron transporter homolog 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 339.7 bits (870), Expect = 1.8e-89
Identity = 192/217 (88.48%), Postives = 200/217 (92.17%), Query Frame = 0

Query: 1   MDPNKLNLSTLEQQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQD 60
           M+PNKLNL+ LEQQS  EIQT EFDYSKRAQWLRAAVLGANDGLVSTASLMMG+GAVKQD
Sbjct: 1   MEPNKLNLTNLEQQSGSEIQTNEFDYSKRAQWLRAAVLGANDGLVSTASLMMGIGAVKQD 60

Query: 61  IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQES 120
           IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIE+AQ+KREKLQR  N+EG    Q Q+S
Sbjct: 61  IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEVAQMKREKLQR-RNLEG----QEQDS 120

Query: 121 KDEEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGA 180
            D+EKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRL VVVAAVTLAL VFGWLGA
Sbjct: 121 NDDEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLVVVVAAVTLALGVFGWLGA 180

Query: 181 ILGKASPIKSASRVLVGGWLAMAITFGLTKLIGASGL 218
           ILGKASPIKSA RVLVGGWLAM ITFGLTKLIG SGL
Sbjct: 181 ILGKASPIKSAIRVLVGGWLAMTITFGLTKLIGVSGL 212

BLAST of HG10004027 vs. ExPASy Swiss-Prot
Match: Q9M2C3 (Vacuolar iron transporter homolog 3 OS=Arabidopsis thaliana OX=3702 GN=At3g43630 PE=2 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 4.4e-70
Identity = 148/195 (75.90%), Postives = 167/195 (85.64%), Query Frame = 0

Query: 20  QTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQDIKAMILTGFAGLVAGACSM 79
           Q K FDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ++K MILTGFAGLVAGACSM
Sbjct: 15  QEKAFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQNVKIMILTGFAGLVAGACSM 74

Query: 80  AIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQESKDEEKLPNPLQAAAASALA 139
           AIGEFVSVYSQ DIE+AQ+KRE          GG +      ++EKLP+P QAAAASALA
Sbjct: 75  AIGEFVSVYSQYDIEVAQMKRET---------GGEI------EKEKLPSPTQAAAASALA 134

Query: 140 FSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGAILGKASPIKSASRVLVGGW 199
           FSLGAMVPLLAA+F++EYKVR+  +VAAVTLALV+FGWLGA+LGKA  +KS+ RVLVGGW
Sbjct: 135 FSLGAMVPLLAAAFVKEYKVRIGAIVAAVTLALVMFGWLGAVLGKAPVVKSSLRVLVGGW 194

Query: 200 LAMAITFGLTKLIGA 215
           LAMAIT+G TKLIG+
Sbjct: 195 LAMAITYGFTKLIGS 194

BLAST of HG10004027 vs. ExPASy Swiss-Prot
Match: Q9LPU9 (Vacuolar iron transporter homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VTL1 PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 5.7e-70
Identity = 146/198 (73.74%), Postives = 168/198 (84.85%), Query Frame = 0

Query: 20  QTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQDIKAMILTGFAGLVAGACSM 79
           Q K FDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQD+K MIL+GFAGLVAGACSM
Sbjct: 18  QEKAFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQDVKVMILSGFAGLVAGACSM 77

Query: 80  AIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQESKDEEKLPNPLQAAAASALA 139
           AIGEFVSVYSQ DIE+AQ+KR         E GG V      ++EKLP+P+QAAAASALA
Sbjct: 78  AIGEFVSVYSQYDIEVAQMKR---------ENGGQV------EKEKLPSPMQAAAASALA 137

Query: 140 FSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGAILGKASPIKSASRVLVGGW 199
           FSLGA+VPL+AA+F+++Y VR+  +VAAVTLALV+FGWLGA+LGKA   KS++RVL+GGW
Sbjct: 138 FSLGAIVPLMAAAFVKDYHVRIGAIVAAVTLALVMFGWLGAVLGKAPVFKSSARVLIGGW 197

Query: 200 LAMAITFGLTKLIGASGL 218
           LAMA+TFGLTKLIG   L
Sbjct: 198 LAMAVTFGLTKLIGTHSL 200

BLAST of HG10004027 vs. ExPASy Swiss-Prot
Match: Q9M2C0 (Vacuolar iron transporter homolog 4 OS=Arabidopsis thaliana OX=3702 GN=At3g43660 PE=2 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 8.2e-69
Identity = 149/217 (68.66%), Postives = 177/217 (81.57%), Query Frame = 0

Query: 1   MDPNKLNLSTLEQQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQD 60
           M+ N  NL+ L+ + +   Q   FDYSKRAQWLRAAVLGANDGLVSTASLMMG+GAVKQD
Sbjct: 1   MESNNNNLN-LDMEKD---QETTFDYSKRAQWLRAAVLGANDGLVSTASLMMGIGAVKQD 60

Query: 61  IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQES 120
           ++ M+LTGFAGLVAGACSMAIGEF+SVYSQ DIE+AQ+KRE               G E+
Sbjct: 61  VRIMLLTGFAGLVAGACSMAIGEFISVYSQYDIEVAQMKRE--------------SGGET 120

Query: 121 KDEEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGA 180
           K +EKLP+P QAA ASALAF+LGA+VPLLAA+F++EYKVR+ V+VAAVTLALV+FGWLGA
Sbjct: 121 K-KEKLPSPTQAAIASALAFTLGAIVPLLAAAFVKEYKVRIGVIVAAVTLALVMFGWLGA 180

Query: 181 ILGKASPIKSASRVLVGGWLAMAITFGLTKLIGASGL 218
           +LGKA  +KS  RVL+GGWLAMAITFG TKL+G+ GL
Sbjct: 181 VLGKAPVVKSLVRVLIGGWLAMAITFGFTKLVGSHGL 198

BLAST of HG10004027 vs. ExPASy Swiss-Prot
Match: Q9SRD3 (Vacuolar iron transporter homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VTL2 PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 1.7e-66
Identity = 138/202 (68.32%), Postives = 169/202 (83.66%), Query Frame = 0

Query: 16  NLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQDIKAMILTGFAGLVAG 75
           ++E ++  FDYSKR+QWLRAAVLGANDGLVSTASLMMGVGAVK D+KAMIL+GFAG+VAG
Sbjct: 11  DIEKESTTFDYSKRSQWLRAAVLGANDGLVSTASLMMGVGAVKHDVKAMILSGFAGMVAG 70

Query: 76  ACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQESKDEEKLPNPLQAAAA 135
           ACSMAIGEFVSVYSQ DIE+AQ++R+ ++                 ++EKLP+P+QAAAA
Sbjct: 71  ACSMAIGEFVSVYSQYDIEVAQMERDSVE----------------IEKEKLPSPMQAAAA 130

Query: 136 SALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGAILGKASPIKSASRVL 195
           SALAFS GA+VPLLAA+F++EYK+R+  VV AVT+AL+VFGWLGA LGKA  ++S++RVL
Sbjct: 131 SALAFSAGAIVPLLAAAFVKEYKMRIISVVVAVTVALMVFGWLGAALGKAPAVRSSARVL 190

Query: 196 VGGWLAMAITFGLTKLIGASGL 218
            GGWLAMA+TFGLTKLIG  GL
Sbjct: 191 FGGWLAMAVTFGLTKLIGLYGL 196

BLAST of HG10004027 vs. ExPASy Swiss-Prot
Match: Q9LSF6 (Vacuolar iron transporter homolog 2.1 OS=Arabidopsis thaliana OX=3702 GN=VTL5 PE=1 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.5e-57
Identity = 124/209 (59.33%), Postives = 161/209 (77.03%), Query Frame = 0

Query: 9   STLEQQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQDIKAMILTG 68
           S   Q++    + +E DY +RAQWLRAA+LGANDGLV+ ASLMMGVG++K+D+KAM+L G
Sbjct: 12  SPRNQKTRPRAEKEEVDYMQRAQWLRAALLGANDGLVTVASLMMGVGSIKEDVKAMLLVG 71

Query: 69  FAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQESKDEEKLPN 128
           FAGLVAGACSMAIGEFVSV +Q DIE AQ+KR    ++S      + + +E + +E+LPN
Sbjct: 72  FAGLVAGACSMAIGEFVSVCTQRDIETAQMKRAIEHKTSL---SAIDEQEEEEKKERLPN 131

Query: 129 PLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGAILGKASPI 188
           P QAA ASALAFS+GA +PLL A FI  +KVR+ VV    T+ALVVFG  GA+LGK S +
Sbjct: 132 PGQAAIASALAFSVGAAMPLLGAVFIENHKVRMVVVAVVATIALVVFGVTGAVLGKTSVV 191

Query: 189 KSASRVLVGGWLAMAITFGLTKLIGASGL 218
           KS+ RV++GGW+AMA+TFGLTK IG++ +
Sbjct: 192 KSSVRVVIGGWMAMALTFGLTKFIGSAAM 217

BLAST of HG10004027 vs. ExPASy TrEMBL
Match: A0A5A7UG57 (Vacuolar iron transporter-like protein 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1163G00440 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 6.1e-99
Identity = 207/218 (94.95%), Postives = 213/218 (97.71%), Query Frame = 0

Query: 1   MDPNKLNLSTLE-QQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ 60
           MDPNKLNL+ LE QQS LEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ
Sbjct: 1   MDPNKLNLNNLEQQQSTLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ 60

Query: 61  DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQE 120
           DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRS+NME GGVVQGQE
Sbjct: 61  DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSNNME-GGVVQGQE 120

Query: 121 SKDEEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLG 180
           SKD+EKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVA+VTLAL VFGWLG
Sbjct: 121 SKDKEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVASVTLALAVFGWLG 180

Query: 181 AILGKASPIKSASRVLVGGWLAMAITFGLTKLIGASGL 218
           A+LGKASPIKSA+RVLVGGWLAMAITFGLTKLIGASGL
Sbjct: 181 AVLGKASPIKSAARVLVGGWLAMAITFGLTKLIGASGL 217

BLAST of HG10004027 vs. ExPASy TrEMBL
Match: A0A1S3B368 (vacuolar iron transporter homolog 4-like OS=Cucumis melo OX=3656 GN=LOC103485624 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 6.1e-99
Identity = 207/218 (94.95%), Postives = 213/218 (97.71%), Query Frame = 0

Query: 1   MDPNKLNLSTLE-QQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ 60
           MDPNKLNL+ LE QQS LEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ
Sbjct: 1   MDPNKLNLNNLEQQQSTLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ 60

Query: 61  DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQE 120
           DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRS+NME GGVVQGQE
Sbjct: 61  DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSNNME-GGVVQGQE 120

Query: 121 SKDEEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLG 180
           SKD+EKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVA+VTLAL VFGWLG
Sbjct: 121 SKDKEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVASVTLALAVFGWLG 180

Query: 181 AILGKASPIKSASRVLVGGWLAMAITFGLTKLIGASGL 218
           A+LGKASPIKSA+RVLVGGWLAMAITFGLTKLIGASGL
Sbjct: 181 AVLGKASPIKSAARVLVGGWLAMAITFGLTKLIGASGL 217

BLAST of HG10004027 vs. ExPASy TrEMBL
Match: A0A0A0KCM7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G057130 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 5.1e-98
Identity = 205/218 (94.04%), Postives = 212/218 (97.25%), Query Frame = 0

Query: 1   MDPNKLNLSTLE-QQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ 60
           MDPNKLNL+ LE QQS LEIQ KEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ
Sbjct: 1   MDPNKLNLNNLEQQQSTLEIQAKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ 60

Query: 61  DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQE 120
           DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRS+NME GGVVQGQ+
Sbjct: 61  DIKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSNNME-GGVVQGQD 120

Query: 121 SKDEEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLG 180
           SKD+EKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVA+VTLAL VFGWLG
Sbjct: 121 SKDKEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVASVTLALAVFGWLG 180

Query: 181 AILGKASPIKSASRVLVGGWLAMAITFGLTKLIGASGL 218
           AILGKASPIKSA+RVL+GGWLAMAITFGLTKLIGASGL
Sbjct: 181 AILGKASPIKSAARVLIGGWLAMAITFGLTKLIGASGL 217

BLAST of HG10004027 vs. ExPASy TrEMBL
Match: A0A6J1K3Q3 (vacuolar iron transporter homolog 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489779 PE=3 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 4.7e-91
Identity = 195/217 (89.86%), Postives = 201/217 (92.63%), Query Frame = 0

Query: 1   MDPNKLNLSTLEQQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQD 60
           M+PNKLNL+ LEQQS  EIQT EFDYSKRAQWLRAAVLGANDGLVSTASLMMG+GAVKQD
Sbjct: 15  MEPNKLNLTNLEQQSGSEIQTNEFDYSKRAQWLRAAVLGANDGLVSTASLMMGIGAVKQD 74

Query: 61  IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQES 120
           IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIE+AQIKREKLQR  N+EG    Q QES
Sbjct: 75  IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEVAQIKREKLQR-RNLEG----QEQES 134

Query: 121 KDEEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGA 180
           KD+EKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRL VVVAAVTLAL VFGWLGA
Sbjct: 135 KDDEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLVVVVAAVTLALGVFGWLGA 194

Query: 181 ILGKASPIKSASRVLVGGWLAMAITFGLTKLIGASGL 218
           ILGKASPIKS  RVLVGGWLAMAITFGLTKLIG SGL
Sbjct: 195 ILGKASPIKSTIRVLVGGWLAMAITFGLTKLIGVSGL 226

BLAST of HG10004027 vs. ExPASy TrEMBL
Match: A0A6J1FFH9 (vacuolar iron transporter homolog 4-like OS=Cucurbita moschata OX=3662 GN=LOC111445258 PE=3 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 1.5e-89
Identity = 191/217 (88.02%), Postives = 201/217 (92.63%), Query Frame = 0

Query: 1   MDPNKLNLSTLEQQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQD 60
           M+PNKLNL+ LE+QS  EIQT EFDYSKRAQWLRAAVLGANDGLVSTASLMMG+GAVKQD
Sbjct: 15  MEPNKLNLTNLERQSGSEIQTNEFDYSKRAQWLRAAVLGANDGLVSTASLMMGIGAVKQD 74

Query: 61  IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQES 120
           IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIE+AQ+KREKLQR  N+EG    Q  ES
Sbjct: 75  IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEVAQMKREKLQR-RNLEG----QELES 134

Query: 121 KDEEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGA 180
           KD+EKLPNPLQAAAASALAFSLGAMVPLLAASFIR+YKVRL VVVAAVTLAL +FGWLGA
Sbjct: 135 KDDEKLPNPLQAAAASALAFSLGAMVPLLAASFIRDYKVRLVVVVAAVTLALAMFGWLGA 194

Query: 181 ILGKASPIKSASRVLVGGWLAMAITFGLTKLIGASGL 218
           ILGKASPIKSA RVLVGGWLAMAITFGLTKLIG SGL
Sbjct: 195 ILGKASPIKSAIRVLVGGWLAMAITFGLTKLIGVSGL 226

BLAST of HG10004027 vs. TAIR 10
Match: AT3G43630.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 265.8 bits (678), Expect = 3.1e-71
Identity = 148/195 (75.90%), Postives = 167/195 (85.64%), Query Frame = 0

Query: 20  QTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQDIKAMILTGFAGLVAGACSM 79
           Q K FDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQ++K MILTGFAGLVAGACSM
Sbjct: 15  QEKAFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQNVKIMILTGFAGLVAGACSM 74

Query: 80  AIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQESKDEEKLPNPLQAAAASALA 139
           AIGEFVSVYSQ DIE+AQ+KRE          GG +      ++EKLP+P QAAAASALA
Sbjct: 75  AIGEFVSVYSQYDIEVAQMKRET---------GGEI------EKEKLPSPTQAAAASALA 134

Query: 140 FSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGAILGKASPIKSASRVLVGGW 199
           FSLGAMVPLLAA+F++EYKVR+  +VAAVTLALV+FGWLGA+LGKA  +KS+ RVLVGGW
Sbjct: 135 FSLGAMVPLLAAAFVKEYKVRIGAIVAAVTLALVMFGWLGAVLGKAPVVKSSLRVLVGGW 194

Query: 200 LAMAITFGLTKLIGA 215
           LAMAIT+G TKLIG+
Sbjct: 195 LAMAITYGFTKLIGS 194

BLAST of HG10004027 vs. TAIR 10
Match: AT1G21140.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 265.4 bits (677), Expect = 4.1e-71
Identity = 146/198 (73.74%), Postives = 168/198 (84.85%), Query Frame = 0

Query: 20  QTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQDIKAMILTGFAGLVAGACSM 79
           Q K FDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQD+K MIL+GFAGLVAGACSM
Sbjct: 18  QEKAFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQDVKVMILSGFAGLVAGACSM 77

Query: 80  AIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQESKDEEKLPNPLQAAAASALA 139
           AIGEFVSVYSQ DIE+AQ+KR         E GG V      ++EKLP+P+QAAAASALA
Sbjct: 78  AIGEFVSVYSQYDIEVAQMKR---------ENGGQV------EKEKLPSPMQAAAASALA 137

Query: 140 FSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGAILGKASPIKSASRVLVGGW 199
           FSLGA+VPL+AA+F+++Y VR+  +VAAVTLALV+FGWLGA+LGKA   KS++RVL+GGW
Sbjct: 138 FSLGAIVPLMAAAFVKDYHVRIGAIVAAVTLALVMFGWLGAVLGKAPVFKSSARVLIGGW 197

Query: 200 LAMAITFGLTKLIGASGL 218
           LAMA+TFGLTKLIG   L
Sbjct: 198 LAMAVTFGLTKLIGTHSL 200

BLAST of HG10004027 vs. TAIR 10
Match: AT3G43660.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 261.5 bits (667), Expect = 5.8e-70
Identity = 149/217 (68.66%), Postives = 177/217 (81.57%), Query Frame = 0

Query: 1   MDPNKLNLSTLEQQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQD 60
           M+ N  NL+ L+ + +   Q   FDYSKRAQWLRAAVLGANDGLVSTASLMMG+GAVKQD
Sbjct: 1   MESNNNNLN-LDMEKD---QETTFDYSKRAQWLRAAVLGANDGLVSTASLMMGIGAVKQD 60

Query: 61  IKAMILTGFAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQES 120
           ++ M+LTGFAGLVAGACSMAIGEF+SVYSQ DIE+AQ+KRE               G E+
Sbjct: 61  VRIMLLTGFAGLVAGACSMAIGEFISVYSQYDIEVAQMKRE--------------SGGET 120

Query: 121 KDEEKLPNPLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGA 180
           K +EKLP+P QAA ASALAF+LGA+VPLLAA+F++EYKVR+ V+VAAVTLALV+FGWLGA
Sbjct: 121 K-KEKLPSPTQAAIASALAFTLGAIVPLLAAAFVKEYKVRIGVIVAAVTLALVMFGWLGA 180

Query: 181 ILGKASPIKSASRVLVGGWLAMAITFGLTKLIGASGL 218
           +LGKA  +KS  RVL+GGWLAMAITFG TKL+G+ GL
Sbjct: 181 VLGKAPVVKSLVRVLIGGWLAMAITFGFTKLVGSHGL 198

BLAST of HG10004027 vs. TAIR 10
Match: AT1G76800.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 253.8 bits (647), Expect = 1.2e-67
Identity = 138/202 (68.32%), Postives = 169/202 (83.66%), Query Frame = 0

Query: 16  NLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQDIKAMILTGFAGLVAG 75
           ++E ++  FDYSKR+QWLRAAVLGANDGLVSTASLMMGVGAVK D+KAMIL+GFAG+VAG
Sbjct: 11  DIEKESTTFDYSKRSQWLRAAVLGANDGLVSTASLMMGVGAVKHDVKAMILSGFAGMVAG 70

Query: 76  ACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQESKDEEKLPNPLQAAAA 135
           ACSMAIGEFVSVYSQ DIE+AQ++R+ ++                 ++EKLP+P+QAAAA
Sbjct: 71  ACSMAIGEFVSVYSQYDIEVAQMERDSVE----------------IEKEKLPSPMQAAAA 130

Query: 136 SALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGAILGKASPIKSASRVL 195
           SALAFS GA+VPLLAA+F++EYK+R+  VV AVT+AL+VFGWLGA LGKA  ++S++RVL
Sbjct: 131 SALAFSAGAIVPLLAAAFVKEYKMRIISVVVAVTVALMVFGWLGAALGKAPAVRSSARVL 190

Query: 196 VGGWLAMAITFGLTKLIGASGL 218
            GGWLAMA+TFGLTKLIG  GL
Sbjct: 191 FGGWLAMAVTFGLTKLIGLYGL 196

BLAST of HG10004027 vs. TAIR 10
Match: AT3G25190.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 224.2 bits (570), Expect = 1.0e-58
Identity = 124/209 (59.33%), Postives = 161/209 (77.03%), Query Frame = 0

Query: 9   STLEQQSNLEIQTKEFDYSKRAQWLRAAVLGANDGLVSTASLMMGVGAVKQDIKAMILTG 68
           S   Q++    + +E DY +RAQWLRAA+LGANDGLV+ ASLMMGVG++K+D+KAM+L G
Sbjct: 12  SPRNQKTRPRAEKEEVDYMQRAQWLRAALLGANDGLVTVASLMMGVGSIKEDVKAMLLVG 71

Query: 69  FAGLVAGACSMAIGEFVSVYSQLDIEMAQIKREKLQRSSNMEGGGVVQGQESKDEEKLPN 128
           FAGLVAGACSMAIGEFVSV +Q DIE AQ+KR    ++S      + + +E + +E+LPN
Sbjct: 72  FAGLVAGACSMAIGEFVSVCTQRDIETAQMKRAIEHKTSL---SAIDEQEEEEKKERLPN 131

Query: 129 PLQAAAASALAFSLGAMVPLLAASFIREYKVRLAVVVAAVTLALVVFGWLGAILGKASPI 188
           P QAA ASALAFS+GA +PLL A FI  +KVR+ VV    T+ALVVFG  GA+LGK S +
Sbjct: 132 PGQAAIASALAFSVGAAMPLLGAVFIENHKVRMVVVAVVATIALVVFGVTGAVLGKTSVV 191

Query: 189 KSASRVLVGGWLAMAITFGLTKLIGASGL 218
           KS+ RV++GGW+AMA+TFGLTK IG++ +
Sbjct: 192 KSSVRVVIGGWMAMALTFGLTKFIGSAAM 217

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008441522.11.3e-9894.95PREDICTED: vacuolar iron transporter homolog 4-like [Cucumis melo] >KAA0054933.1... [more]
XP_038885661.18.1e-9895.41vacuolar iron transporter homolog 4-like [Benincasa hispida][more]
XP_004140970.11.1e-9794.04LOW QUALITY PROTEIN: vacuolar iron transporter homolog 4 [Cucumis sativus] >XP_0... [more]
XP_022993928.19.6e-9189.86vacuolar iron transporter homolog 4-like [Cucurbita maxima][more]
XP_023551056.11.8e-8988.48vacuolar iron transporter homolog 4-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9M2C34.4e-7075.90Vacuolar iron transporter homolog 3 OS=Arabidopsis thaliana OX=3702 GN=At3g43630... [more]
Q9LPU95.7e-7073.74Vacuolar iron transporter homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VTL1 PE=2... [more]
Q9M2C08.2e-6968.66Vacuolar iron transporter homolog 4 OS=Arabidopsis thaliana OX=3702 GN=At3g43660... [more]
Q9SRD31.7e-6668.32Vacuolar iron transporter homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VTL2 PE=2... [more]
Q9LSF61.5e-5759.33Vacuolar iron transporter homolog 2.1 OS=Arabidopsis thaliana OX=3702 GN=VTL5 PE... [more]
Match NameE-valueIdentityDescription
A0A5A7UG576.1e-9994.95Vacuolar iron transporter-like protein 4-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3B3686.1e-9994.95vacuolar iron transporter homolog 4-like OS=Cucumis melo OX=3656 GN=LOC103485624... [more]
A0A0A0KCM75.1e-9894.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G057130 PE=3 SV=1[more]
A0A6J1K3Q34.7e-9189.86vacuolar iron transporter homolog 4-like OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A6J1FFH91.5e-8988.02vacuolar iron transporter homolog 4-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
Match NameE-valueIdentityDescription
AT3G43630.13.1e-7175.90Vacuolar iron transporter (VIT) family protein [more]
AT1G21140.14.1e-7173.74Vacuolar iron transporter (VIT) family protein [more]
AT3G43660.15.8e-7068.66Vacuolar iron transporter (VIT) family protein [more]
AT1G76800.11.2e-6768.32Vacuolar iron transporter (VIT) family protein [more]
AT3G25190.11.0e-5859.33Vacuolar iron transporter (VIT) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008217Ccc1 familyPFAMPF01988VIT1coord: 122..209
e-value: 3.8E-19
score: 69.4
coord: 33..108
e-value: 1.0E-25
score: 91.0
IPR008217Ccc1 familyPANTHERPTHR31851FE(2+)/MN(2+) TRANSPORTER PCL1coord: 18..214
NoneNo IPR availablePANTHERPTHR31851:SF42VACUOLAR IRON TRANSPORTER HOMOLOG 1-RELATEDcoord: 18..214

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10004027.1HG10004027.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030026 cellular manganese ion homeostasis
biological_process GO:0006880 intracellular sequestering of iron ion
biological_process GO:0034755 iron ion transmembrane transport
biological_process GO:0071421 manganese ion transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005381 iron ion transmembrane transporter activity
molecular_function GO:0005384 manganese ion transmembrane transporter activity