HG10003735 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10003735
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionGTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic
LocationChr08: 7568285 .. 7572158 (+)
RNA-Seq ExpressionHG10003735
SyntenyHG10003735
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGTTTTGTTATCTGCAATGGAGCTCAATCTGAAACTCCCCCACAATAATTTCTGTCATGGACTCAACATACGAACACGACCTCCTTTCTTTCCAGGTAAATTCCTCTTTTTCCCAGCATTTTCCTTTACACTCCGATTCATAGAGTAAACGAAAACCCCTCTTTTCGTAGGCCTCAATAGACAGGAAACTGCCCACAAAAGTCGAATGCAACCCATTAGCTTAGCCGTGAAATCCCAACTAACTGTTCACACAACAAGTACTAAGATTCAGGGAAAAGGCAGAACAAAAAATCCTGTTCTGAGTGAGGGAAGAGATGAGGATGAGCAAAATGGAGACATATGTCCTGGCTGTGGAGTTTTTATGCAAGATGAGGATCCAAACTTTCTTGGGTTTTATCAGAAAAGGAAGGTTTCACTCACAGAGGCAATGGAAGATGGGGAGGATGTGGAAGATGAGTTTTATGGAGTTGTAGACGATGATGTTGTGGATGAAGAAGAAAATAGTGATGGACAAGAAATTGGGGATGGATTTGATTGGGATTCAGATGAATGGGAGGCTAAGCTCATGAAGGAAGAAGAAAATGATTTGGAATTGGATGGTTTTGCTCCTGCTGATGTGGGATATGGAAACATAACAGAGGAAACAGTGAAGAGAGCTGAGAAGAAGAGAATATCTAAGTCAGAAAAGAAGAGGAGAGCTAGAGAGGCTCAGAAAGAGATAGAAGAGGTAACAGTTTGTGCAAGGTGCCATTCATTGAGGAATTATGGGCAGGTGAAGAACCAAGCTGCTGAGAACTTGATACCTGATTTTGATTTTGATAGATTGATAGCAAACCGTTTGATGAAATCCACATCAAATTTGAATAATGTTGTTGTTATGGTTGTTGATTGTGTTGATTTCGATGGTTCTTTCCCGAAGCGTGCAGCAAAATCATTGTTTAAGGCCTTGGAAGGGACTAAAAACAACCCCAAGATGAGTAAAAAGTTGCCAAAGCTTGTTCTTGTGGCCACAAAGGTTGACCTCCTCCCATCACAGATTTCACCCACAAGACTAGATAGATGGGTTCGGCACCGTGCTAAGGCTGCGGGTGCGCCTAAATTAACTAGTGTTTATTTGGTTAGTTCTCGTAAAGATGTCGGCGTGAGAAATCTGTTGTCCTTCATCAAAGAATTGGCTGGTCCGCGAGGAAATGTGTGGGTTATTGGTGCTCAGAATGCTGGGAAGTCTACTCTCATTAACGCACTAGCAAAAAAAGAAAGGGCTAAAGTGACAAAGCTTACAGAAGCTCCAATTCCTGGGACTACGCTCGGGATCTTGAGAATTGCGGGGATTTTGTCAGCCCAGGCAAAGTTGTTTGATACTCCTGGGCTTCTGCATCCTTACCTAGTGTCCATGAGATTGAATAGGGAAGAACAGAAGATGGTCGAAATCCGAAAGGAGCTCCAACCCAGGACTTACAGAGTGAAGGCAAGTTGTGCTTTTATGAGTATGAATATATGTATACCTGTTTTCAGTGATAACATGGATTTATTCTATCATCTAGTTGACTATATTCAGTACTTAACTAATCACATTTATGATCTCTATATTTTAAGTCCACATTGAGTTCTAAATGATAGCATGATAAATACTTAAGAATCTACTCTATTATACTGCACTTATTTTTAGGATCAATTAAGCGACCCCTCAATTGGAGAATGTTAATTTTTCTTGCTCGTTAATTGATTCATTTGTATTAATTGGAATTATTTTCTCTTTGATTCGTTAATTGATGTTATGAACCATCTTGGGTTGGCCTAGTGGTGGGAACATCCAAAAATGGCCAAAGGGCTAAGGGGTCATGAGTTCAATCCATAGTAGCCACCTACCTAGGATTTAATATCCTACAGGTGTCTTTGACACCCAATGTTGTAGGGTCAGGCGGGTTGTCCCATAAGTTGGCTGGCCCGAACACTCAAAGATATTAAAAAGAAAAAAAGAAAAAAACTCTTTTAAGTCATGATTACTTCGATTAAGCTCATGGGATACTCTGCACTTTTTATTATAACTTCCATAGAAAAATTTATGGTCTAGTGTAGGCAAAATGAGTAAACTGATATCATACTATAGTTGTAGTGGTATATTATTGTATAGTTAGTCAATGTTTACCAAAATGGATTGTAGTTATTGCTTCAAAATAGTTGATAGGCCTTCATTTTGTATGCAAATAAAAATGGTTAAATTACCAAAAAAAAAAAAAAAATCCTAAATTTTGCCCATTGTTTAAAAAAATGCTCATATTCTTTCAAAAGTTATATTACTTTAGACCTTTCATAAATATTTCAAAACTACCCTCTGTGAGAAAATTTGTTTGAATTTTGGATGAGAATTAGAACCTGAAATGGGTGGGATAATAGCCGTGATGGATAGTGTGGTGCTATTTAGGTCCCAATTTCCATATTGGGTCACCACGACACCATGTCAGATCCATTTTTTGTCTAACATTCTTACAAATTTCTACTCCAAGGTAGTTTTGAAACCTTTATGAAAGTTTAAGGGTAATTATATTGCAACTTTTGAAATAATAATGATATTTTTTAAACAAAGGGCATAGCCAATAAAAATTAGTCACCTTACACATTTTTCTTGATTGTGGCTAGTTAGTTTCAACAATTCACTTGACTAGTTTTTACTGGTGGACAAGCATCAACTTATACAGATTTGGTTAATGTTGCTGTTCACTAGATAACTGGATGCTGTCGGACTCTAATTATAACTAACAGTATTGGGCAGGTAGGGCAGGCTGTACATGTTGGTGGTTTGGTAAGACTTGACCTTAACCAAGCTTCAGTAGAGACAATATATGTTACAGTTTGGGCATCACCAAATGTTTCTCTTCACTTGGGGAAGATTGAAAATGCTGATGAGATATGGAAGAAACATGCTGGCATAAGGTTGCAGGTAATTGGCTTTGTTTCACTCAATTCACATTTCACAAAAGAAATGTTGTTATTTCCAGAATGTCTACTATAATGCATTTCTCTCCATTATTAGTTTCTCTGGGGATATTTGTTGTGCTTGCTAACGTATTATCTTATCCATCTTATGCCATTTCTTTTCAATCATTTTAGTTCACTGCAATCAATATCTAGAACAACTAACATTCATCTCCTAAAAATTGCATTGATTTGATAGTGAGGAGGGGCAATTGATTTTGAATGATTAAAGTATGTTTCGGAGTGATTTTGAATATGAAAAAAGTGATCTTTAACCTTTCAAAATCACTCACGAGAAGTAATACCATATAATCGTAAATATCAGATTCTGTCAAAAAATTATATGATATTCCTTGTTATACAAATATGCATAAGGAAGCAAGTAAAAAGTACTTGAAAACACTTTATTTCAAACATGAATGAAGTGCTTTTGGTCCTGAAAGAACTCCTAGATTTAGCTATATTAGGTGGAAGGAAAGGAAAATAAAGTATTTTATGATTGTCAGTTGATCAATATTTGTTTTTCTTGTTTCTTCTAGCCGCCCATTGGCGTCGACCGTGCTTCTGAAATTGGCAAATGGGAAGAGAAAGAGGTCAAAATTTCTGGCACAAGTTGGGTTGTCAATAGCATTGATATTTCAATAGCAGGTTTAGGCTGGTTTTCTTTAGGTCTCAAAGGTGAAGCAACCTTGACATTGTGGATATACGATGGGATGGAAGTAACGTTGAGAGAACCGTTGGTTCTTGATCGATCACCATTCCTTGAGAGACCCGGCTTCTGGCTACCGAAAGCCATATCGAACACCATTGGCAATGAAACAAAATTTGATGCACAGAGGAGAAACTCCCTAGAGGAGGAGAGTGCAAAGCCCATTGTGCAAGTTTCCACATGA

mRNA sequence

ATGGCAGTTTTGTTATCTGCAATGGAGCTCAATCTGAAACTCCCCCACAATAATTTCTGTCATGGACTCAACATACGAACACGACCTCCTTTCTTTCCAGGCCTCAATAGACAGGAAACTGCCCACAAAAGTCGAATGCAACCCATTAGCTTAGCCGTGAAATCCCAACTAACTGTTCACACAACAAGTACTAAGATTCAGGGAAAAGGCAGAACAAAAAATCCTGTTCTGAGTGAGGGAAGAGATGAGGATGAGCAAAATGGAGACATATGTCCTGGCTGTGGAGTTTTTATGCAAGATGAGGATCCAAACTTTCTTGGGTTTTATCAGAAAAGGAAGGTTTCACTCACAGAGGCAATGGAAGATGGGGAGGATGTGGAAGATGAGTTTTATGGAGTTGTAGACGATGATGTTGTGGATGAAGAAGAAAATAGTGATGGACAAGAAATTGGGGATGGATTTGATTGGGATTCAGATGAATGGGAGGCTAAGCTCATGAAGGAAGAAGAAAATGATTTGGAATTGGATGGTTTTGCTCCTGCTGATGTGGGATATGGAAACATAACAGAGGAAACAGTGAAGAGAGCTGAGAAGAAGAGAATATCTAAGTCAGAAAAGAAGAGGAGAGCTAGAGAGGCTCAGAAAGAGATAGAAGAGGTAACAGTTTGTGCAAGGTGCCATTCATTGAGGAATTATGGGCAGGTGAAGAACCAAGCTGCTGAGAACTTGATACCTGATTTTGATTTTGATAGATTGATAGCAAACCGTTTGATGAAATCCACATCAAATTTGAATAATGTTGTTGTTATGGTTGTTGATTGTGTTGATTTCGATGGTTCTTTCCCGAAGCGTGCAGCAAAATCATTGTTTAAGGCCTTGGAAGGGACTAAAAACAACCCCAAGATGAGTAAAAAGTTGCCAAAGCTTGTTCTTGTGGCCACAAAGGTTGACCTCCTCCCATCACAGATTTCACCCACAAGACTAGATAGATGGGTTCGGCACCGTGCTAAGGCTGCGGGTGCGCCTAAATTAACTAGTGTTTATTTGGTTAGTTCTCGTAAAGATGTCGGCGTGAGAAATCTGTTGTCCTTCATCAAAGAATTGGCTGGTCCGCGAGGAAATGTGTGGGTTATTGGTGCTCAGAATGCTGGGAAGTCTACTCTCATTAACGCACTAGCAAAAAAAGAAAGGGCTAAAGTGACAAAGCTTACAGAAGCTCCAATTCCTGGGACTACGCTCGGGATCTTGAGAATTGCGGGGATTTTGTCAGCCCAGGCAAAGTTGTTTGATACTCCTGGGCTTCTGCATCCTTACCTAGTGTCCATGAGATTGAATAGGGAAGAACAGAAGATGGTCGAAATCCGAAAGGAGCTCCAACCCAGGACTTACAGAGTGAAGGCAAGTTGTGCTTTTATGAGGCAGGCTGTACATGTTGGTGGTTTGGTAAGACTTGACCTTAACCAAGCTTCAGTAGAGACAATATATGTTACAGTTTGGGCATCACCAAATGTTTCTCTTCACTTGGGGAAGATTGAAAATGCTGATGAGATATGGAAGAAACATGCTGGCATAAGGTTGCAGCCGCCCATTGGCGTCGACCGTGCTTCTGAAATTGGCAAATGGGAAGAGAAAGAGGTCAAAATTTCTGGCACAAGTTGGGTTGTCAATAGCATTGATATTTCAATAGCAGGTTTAGGCTGGTTTTCTTTAGGTCTCAAAGGTGAAGCAACCTTGACATTGTGGATATACGATGGGATGGAAGTAACGTTGAGAGAACCGTTGGTTCTTGATCGATCACCATTCCTTGAGAGACCCGGCTTCTGGCTACCGAAAGCCATATCGAACACCATTGGCAATGAAACAAAATTTGATGCACAGAGGAGAAACTCCCTAGAGGAGGAGAGTGCAAAGCCCATTGTGCAAGTTTCCACATGA

Coding sequence (CDS)

ATGGCAGTTTTGTTATCTGCAATGGAGCTCAATCTGAAACTCCCCCACAATAATTTCTGTCATGGACTCAACATACGAACACGACCTCCTTTCTTTCCAGGCCTCAATAGACAGGAAACTGCCCACAAAAGTCGAATGCAACCCATTAGCTTAGCCGTGAAATCCCAACTAACTGTTCACACAACAAGTACTAAGATTCAGGGAAAAGGCAGAACAAAAAATCCTGTTCTGAGTGAGGGAAGAGATGAGGATGAGCAAAATGGAGACATATGTCCTGGCTGTGGAGTTTTTATGCAAGATGAGGATCCAAACTTTCTTGGGTTTTATCAGAAAAGGAAGGTTTCACTCACAGAGGCAATGGAAGATGGGGAGGATGTGGAAGATGAGTTTTATGGAGTTGTAGACGATGATGTTGTGGATGAAGAAGAAAATAGTGATGGACAAGAAATTGGGGATGGATTTGATTGGGATTCAGATGAATGGGAGGCTAAGCTCATGAAGGAAGAAGAAAATGATTTGGAATTGGATGGTTTTGCTCCTGCTGATGTGGGATATGGAAACATAACAGAGGAAACAGTGAAGAGAGCTGAGAAGAAGAGAATATCTAAGTCAGAAAAGAAGAGGAGAGCTAGAGAGGCTCAGAAAGAGATAGAAGAGGTAACAGTTTGTGCAAGGTGCCATTCATTGAGGAATTATGGGCAGGTGAAGAACCAAGCTGCTGAGAACTTGATACCTGATTTTGATTTTGATAGATTGATAGCAAACCGTTTGATGAAATCCACATCAAATTTGAATAATGTTGTTGTTATGGTTGTTGATTGTGTTGATTTCGATGGTTCTTTCCCGAAGCGTGCAGCAAAATCATTGTTTAAGGCCTTGGAAGGGACTAAAAACAACCCCAAGATGAGTAAAAAGTTGCCAAAGCTTGTTCTTGTGGCCACAAAGGTTGACCTCCTCCCATCACAGATTTCACCCACAAGACTAGATAGATGGGTTCGGCACCGTGCTAAGGCTGCGGGTGCGCCTAAATTAACTAGTGTTTATTTGGTTAGTTCTCGTAAAGATGTCGGCGTGAGAAATCTGTTGTCCTTCATCAAAGAATTGGCTGGTCCGCGAGGAAATGTGTGGGTTATTGGTGCTCAGAATGCTGGGAAGTCTACTCTCATTAACGCACTAGCAAAAAAAGAAAGGGCTAAAGTGACAAAGCTTACAGAAGCTCCAATTCCTGGGACTACGCTCGGGATCTTGAGAATTGCGGGGATTTTGTCAGCCCAGGCAAAGTTGTTTGATACTCCTGGGCTTCTGCATCCTTACCTAGTGTCCATGAGATTGAATAGGGAAGAACAGAAGATGGTCGAAATCCGAAAGGAGCTCCAACCCAGGACTTACAGAGTGAAGGCAAGTTGTGCTTTTATGAGGCAGGCTGTACATGTTGGTGGTTTGGTAAGACTTGACCTTAACCAAGCTTCAGTAGAGACAATATATGTTACAGTTTGGGCATCACCAAATGTTTCTCTTCACTTGGGGAAGATTGAAAATGCTGATGAGATATGGAAGAAACATGCTGGCATAAGGTTGCAGCCGCCCATTGGCGTCGACCGTGCTTCTGAAATTGGCAAATGGGAAGAGAAAGAGGTCAAAATTTCTGGCACAAGTTGGGTTGTCAATAGCATTGATATTTCAATAGCAGGTTTAGGCTGGTTTTCTTTAGGTCTCAAAGGTGAAGCAACCTTGACATTGTGGATATACGATGGGATGGAAGTAACGTTGAGAGAACCGTTGGTTCTTGATCGATCACCATTCCTTGAGAGACCCGGCTTCTGGCTACCGAAAGCCATATCGAACACCATTGGCAATGAAACAAAATTTGATGCACAGAGGAGAAACTCCCTAGAGGAGGAGAGTGCAAAGCCCATTGTGCAAGTTTCCACATGA

Protein sequence

MAVLLSAMELNLKLPHNNFCHGLNIRTRPPFFPGLNRQETAHKSRMQPISLAVKSQLTVHTTSTKIQGKGRTKNPVLSEGRDEDEQNGDICPGCGVFMQDEDPNFLGFYQKRKVSLTEAMEDGEDVEDEFYGVVDDDVVDEEENSDGQEIGDGFDWDSDEWEAKLMKEEENDLELDGFAPADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAAENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEGTKNNPKMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTSVYLVSSRKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAGILSAQAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKASCAFMRQAVHVGGLVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGKWEEKEVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIYDGMEVTLREPLVLDRSPFLERPGFWLPKAISNTIGNETKFDAQRRNSLEEESAKPIVQVST
Homology
BLAST of HG10003735 vs. NCBI nr
Match: XP_038885390.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 589/640 (92.03%), Postives = 608/640 (95.00%), Query Frame = 0

Query: 1   MAVLLSAMELNLKLPHNNFCHGLNIRTRPPFFPGLNRQETAHKSRMQPISLAVKSQLTVH 60
           MA+LLSAMELNLKLPHNNFCH LNIRTRPPFFPGLNRQ TAHK++ QPISLAVKSQL VH
Sbjct: 1   MAILLSAMELNLKLPHNNFCHRLNIRTRPPFFPGLNRQGTAHKNQTQPISLAVKSQLIVH 60

Query: 61  TTSTKIQGKGRTKNPVLSEGRDEDEQNGDICPGCGVFMQDEDPNFLGFYQKRKVSLTEAM 120
            TSTK+ GK RTKNPVLSEGRDEDEQNGD+CPGCGVFMQDEDPN LG+YQKRKVSLTE M
Sbjct: 61  -TSTKVHGKARTKNPVLSEGRDEDEQNGDVCPGCGVFMQDEDPNLLGYYQKRKVSLTEPM 120

Query: 121 EDGEDVEDEFYGVVDDDVVDEEENSDGQEIGDGFDWDSDEWEAKLMKEEENDLELDGFAP 180
           E GEDVED+F GV DD VVDEEE+SD +EIGDG DWDSDEWEAKLM+EEENDLELDGFAP
Sbjct: 121 EAGEDVEDDFLGVADDHVVDEEESSDEEEIGDGLDWDSDEWEAKLMEEEENDLELDGFAP 180

Query: 181 ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA 240
           ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA
Sbjct: 181 ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA 240

Query: 241 ENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEGTKNNP 300
           ENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFK LEG KN+P
Sbjct: 241 ENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKTLEGNKNDP 300

Query: 301 KMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTSVYLVSSRKDVGVRN 360
           KMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLT VYLVSSRKDVGVRN
Sbjct: 301 KMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTGVYLVSSRKDVGVRN 360

Query: 361 LLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG 420
           LLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG
Sbjct: 361 LLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG 420

Query: 421 ILSAQAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKASCAFMRQAVHVGG 480
           ILSA+AKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVK       QAVHVGG
Sbjct: 421 ILSAKAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKVG-----QAVHVGG 480

Query: 481 LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK 540
           LVRLDLNQASVETIYVTVW SPNVSLHLGKIENADEIWKKHAG RLQPPIG+DRASEIGK
Sbjct: 481 LVRLDLNQASVETIYVTVWTSPNVSLHLGKIENADEIWKKHAGTRLQPPIGIDRASEIGK 540

Query: 541 WEEKEVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIYDGMEVTLREPLVLDRSP 600
           WEE+EVKISGTSWVVNSIDISIAGLGWFSLGLKGEA+LTLWIYDGMEVTLREPLVLDR+P
Sbjct: 541 WEEREVKISGTSWVVNSIDISIAGLGWFSLGLKGEASLTLWIYDGMEVTLREPLVLDRAP 600

Query: 601 FLERPGFWLPKAISNTIGNETKFDAQRRNSLEEESAKPIV 641
           FLERPGFWLPKAISNTI N+TK DAQ R S+EEES +PIV
Sbjct: 601 FLERPGFWLPKAISNTIANKTKLDAQ-RISVEEESVEPIV 633

BLAST of HG10003735 vs. NCBI nr
Match: KAE8646627.1 (hypothetical protein Csa_005632 [Cucumis sativus])

HSP 1 Score: 1139.0 bits (2945), Expect = 0.0e+00
Identity = 586/653 (89.74%), Postives = 609/653 (93.26%), Query Frame = 0

Query: 1   MAVLLSAMELNLKLPHNNFCHGLNIRTRPPFFPGLNRQETAHKSRMQPISLAVKSQLTVH 60
           MA+LLSAMELNLKLP NNF HGLNIRTRPPF PG NRQ TAH+ + QPISLAVKSQ  V 
Sbjct: 1   MAILLSAMELNLKLPVNNFYHGLNIRTRPPFLPGPNRQVTAHRIQFQPISLAVKSQQIVR 60

Query: 61  TTSTKIQGKGRTKNPVLSEGRDEDEQNGDICPGCGVFMQDEDPNFLGFYQKRKVSLTEAM 120
           TTS+KIQGKGRTK+ VLSEGRDEDEQNGDICPGCGVFMQDEDPN LGFYQKRKVSLTE M
Sbjct: 61  TTSSKIQGKGRTKDSVLSEGRDEDEQNGDICPGCGVFMQDEDPNVLGFYQKRKVSLTEPM 120

Query: 121 EDGEDVEDEFYGVVD---------DDVVDEEENSDGQEIGDGFDWDSDEWEAKLMKEEEN 180
           EDGEDVEDEFYG+VD          DVVDEEENSD +EI DGFDWDSDEWEAKLM++EEN
Sbjct: 121 EDGEDVEDEFYGIVDSDVVDEEENSDVVDEEENSDAEEIEDGFDWDSDEWEAKLMEDEEN 180

Query: 181 DLELDGFAPADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRN 240
           +LELDGFAPADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRN
Sbjct: 181 NLELDGFAPADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRN 240

Query: 241 YGQVKNQAAENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFK 300
           YGQVKNQAAENLIPDFDFDRL+ANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFK
Sbjct: 241 YGQVKNQAAENLIPDFDFDRLMANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFK 300

Query: 301 ALEGTKNNPKMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTSVYLVS 360
           ALEG KN+PKM KKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKL  VYLVS
Sbjct: 301 ALEGNKNDPKMGKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLAGVYLVS 360

Query: 361 SRKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGT 420
           SRKDVGV+NLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGT
Sbjct: 361 SRKDVGVKNLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGT 420

Query: 421 TLGILRIAGILSAQAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKASCAF 480
           TLGILRI GILSA+AKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKA    
Sbjct: 421 TLGILRIGGILSAKAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKA---- 480

Query: 481 MRQAVHVGGLVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIG 540
            RQ VHVGGLVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIG
Sbjct: 481 -RQTVHVGGLVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIG 540

Query: 541 VDRASEIGKWEEKEVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIYDGMEVTLR 600
           VDRASEIGKW E+EVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWI +G+EVT+R
Sbjct: 541 VDRASEIGKWAEREVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIDNGIEVTMR 600

Query: 601 EPLVLDRSPFLERPGFWLPKAISNTIGNETKFDAQRRNSLEEESAKPIVQVST 645
           EPLVLDR+ F+ERPGFWL KAISNTIGNETK DAQRR S+EEESA+P V+ ST
Sbjct: 601 EPLVLDRATFIERPGFWLSKAISNTIGNETKLDAQRRISVEEESAEPFVRAST 648

BLAST of HG10003735 vs. NCBI nr
Match: XP_011656565.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Cucumis sativus])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 584/653 (89.43%), Postives = 607/653 (92.96%), Query Frame = 0

Query: 1   MAVLLSAMELNLKLPHNNFCHGLNIRTRPPFFPGLNRQETAHKSRMQPISLAVKSQLTVH 60
           MA+LLSAMELNLKLP NNF HGLNIRTRPPF PG NRQ TAH+ + QPISLAVKSQ  V 
Sbjct: 1   MAILLSAMELNLKLPVNNFYHGLNIRTRPPFLPGPNRQVTAHRIQFQPISLAVKSQQIVR 60

Query: 61  TTSTKIQGKGRTKNPVLSEGRDEDEQNGDICPGCGVFMQDEDPNFLGFYQKRKVSLTEAM 120
           TTS+KIQGKGRTK+ VLSEGRDEDEQNGDICPGCGVFMQDEDPN LGFYQKRKVSLTE M
Sbjct: 61  TTSSKIQGKGRTKDSVLSEGRDEDEQNGDICPGCGVFMQDEDPNVLGFYQKRKVSLTEPM 120

Query: 121 EDGEDVEDEFYGVVD---------DDVVDEEENSDGQEIGDGFDWDSDEWEAKLMKEEEN 180
           EDGEDVEDEFYG+VD          DVVDEEENSD +EI DGFDWDSDEWEAKLM++EEN
Sbjct: 121 EDGEDVEDEFYGIVDSDVVDEEENSDVVDEEENSDAEEIEDGFDWDSDEWEAKLMEDEEN 180

Query: 181 DLELDGFAPADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRN 240
           +LELDGFAPADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRN
Sbjct: 181 NLELDGFAPADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRN 240

Query: 241 YGQVKNQAAENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFK 300
           YGQVKNQAAENLIPDFDFDRL+ANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFK
Sbjct: 241 YGQVKNQAAENLIPDFDFDRLMANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFK 300

Query: 301 ALEGTKNNPKMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTSVYLVS 360
           ALEG KN+PKM KKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKL  VYLVS
Sbjct: 301 ALEGNKNDPKMGKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLAGVYLVS 360

Query: 361 SRKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGT 420
           SRKDVGV+NLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGT
Sbjct: 361 SRKDVGVKNLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGT 420

Query: 421 TLGILRIAGILSAQAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKASCAF 480
           TLGILRI GILSA+AKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVK     
Sbjct: 421 TLGILRIGGILSAKAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKVG--- 480

Query: 481 MRQAVHVGGLVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIG 540
             Q VHVGGLVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIG
Sbjct: 481 --QTVHVGGLVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIG 540

Query: 541 VDRASEIGKWEEKEVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIYDGMEVTLR 600
           VDRASEIGKW E+EVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWI +G+EVT+R
Sbjct: 541 VDRASEIGKWAEREVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIDNGIEVTMR 600

Query: 601 EPLVLDRSPFLERPGFWLPKAISNTIGNETKFDAQRRNSLEEESAKPIVQVST 645
           EPLVLDR+ F+ERPGFWL KAISNTIGNETK DAQRR S+EEESA+P V+ ST
Sbjct: 601 EPLVLDRATFIERPGFWLSKAISNTIGNETKLDAQRRISVEEESAEPFVRAST 648

BLAST of HG10003735 vs. NCBI nr
Match: XP_008445763.1 (PREDICTED: GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Cucumis melo] >TYK22013.1 GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 582/644 (90.37%), Postives = 606/644 (94.10%), Query Frame = 0

Query: 1   MAVLLSAMELNLKLPHNNFCHGLNIRTRPPFFPGLNRQETAHKSRMQPISLAVKSQLTVH 60
           MA+LLSAMELNLKLP NNF  GLN+RTRPPFFPG NR  TAHK + QPISLAVKSQ  V 
Sbjct: 1   MAILLSAMELNLKLPDNNFYRGLNVRTRPPFFPGPNRLGTAHKIQFQPISLAVKSQQIVR 60

Query: 61  TTSTKIQGKGRTKNPVLSEGRDEDEQNGDICPGCGVFMQDEDPNFLGFYQKRKVSLTEAM 120
            TS+KIQGKGRTK+ VLSEGRDEDEQNGDICPGCGVFMQDEDPN LGFYQKRKVSL+E M
Sbjct: 61  ATSSKIQGKGRTKDSVLSEGRDEDEQNGDICPGCGVFMQDEDPNLLGFYQKRKVSLSEPM 120

Query: 121 EDGEDVEDEFYGVVDDDVVDEEENSDGQEIGDGFDWDSDEWEAKLMKEEENDLELDGFAP 180
           ED EDVEDEFYG++D DVVDEEENSDG+EI D FDWD+DEWEAKLM E+ENDLELDGFAP
Sbjct: 121 EDVEDVEDEFYGILDSDVVDEEENSDGEEIEDDFDWDTDEWEAKLM-EDENDLELDGFAP 180

Query: 181 ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA 240
           ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA
Sbjct: 181 ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA 240

Query: 241 ENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEGTKNNP 300
           ENLIPDFDFDRL+ANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEG KN+P
Sbjct: 241 ENLIPDFDFDRLMANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEGNKNDP 300

Query: 301 KMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTSVYLVSSRKDVGVRN 360
           K+SKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLT VYLVSSRKDVGVRN
Sbjct: 301 KLSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTGVYLVSSRKDVGVRN 360

Query: 361 LLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG 420
           LLS IK+LAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG
Sbjct: 361 LLSLIKQLAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG 420

Query: 421 ILSAQAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKASCAFMRQAVHVGG 480
           ILSA+AKLFDTPGLLHPYL+SMRLNREEQKMVEIRKELQPRTYRVK       Q VHVGG
Sbjct: 421 ILSAKAKLFDTPGLLHPYLMSMRLNREEQKMVEIRKELQPRTYRVKVG-----QTVHVGG 480

Query: 481 LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK 540
           LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK
Sbjct: 481 LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK 540

Query: 541 WEEKEVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIYDGMEVTLREPLVLDRSP 600
           W E+EVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWI +G+EVTLREPLVLDR+ 
Sbjct: 541 WAEREVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIDNGIEVTLREPLVLDRAL 600

Query: 601 FLERPGFWLPKAISNTIGNETKFDAQRRNSLEEESAKPIVQVST 645
           FLERPGFWL KAISNTIGNETK DAQRR S+EEESA+PIV+ ST
Sbjct: 601 FLERPGFWLSKAISNTIGNETKLDAQRRISVEEESAEPIVRAST 638

BLAST of HG10003735 vs. NCBI nr
Match: KAA0032955.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 581/644 (90.22%), Postives = 605/644 (93.94%), Query Frame = 0

Query: 1   MAVLLSAMELNLKLPHNNFCHGLNIRTRPPFFPGLNRQETAHKSRMQPISLAVKSQLTVH 60
           MA+LLSAMELNLKLP NNF  GLN+RTRPPFFPG NR  TAHK + QPISLAVKSQ  V 
Sbjct: 1   MAILLSAMELNLKLPDNNFYRGLNVRTRPPFFPGPNRLGTAHKIQFQPISLAVKSQQIVR 60

Query: 61  TTSTKIQGKGRTKNPVLSEGRDEDEQNGDICPGCGVFMQDEDPNFLGFYQKRKVSLTEAM 120
            TS+KIQGKGRTK+ VLSEGRDEDEQNGDICPGCGVFMQDEDPN LGFYQKRKVSL+E M
Sbjct: 61  ATSSKIQGKGRTKDSVLSEGRDEDEQNGDICPGCGVFMQDEDPNLLGFYQKRKVSLSEPM 120

Query: 121 EDGEDVEDEFYGVVDDDVVDEEENSDGQEIGDGFDWDSDEWEAKLMKEEENDLELDGFAP 180
           ED EDVEDEFYG++D DVVDEEENSDG+EI D FDWD+DEWEAKLM E+ENDLELDGFAP
Sbjct: 121 EDVEDVEDEFYGILDSDVVDEEENSDGEEIEDDFDWDTDEWEAKLM-EDENDLELDGFAP 180

Query: 181 ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA 240
           ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA
Sbjct: 181 ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA 240

Query: 241 ENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEGTKNNP 300
           ENLIPDFDFDRL+ANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEG KN+P
Sbjct: 241 ENLIPDFDFDRLMANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEGNKNDP 300

Query: 301 KMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTSVYLVSSRKDVGVRN 360
           K+SKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLT VYLVSSRKDVGVRN
Sbjct: 301 KLSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTGVYLVSSRKDVGVRN 360

Query: 361 LLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG 420
           LLS IK+LAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG
Sbjct: 361 LLSLIKQLAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG 420

Query: 421 ILSAQAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKASCAFMRQAVHVGG 480
           ILSA+AKLFDTPGLLHPYL+SMRLNREEQKMVEIRKELQPRTYRV        Q VHVGG
Sbjct: 421 ILSAKAKLFDTPGLLHPYLMSMRLNREEQKMVEIRKELQPRTYRV-------GQTVHVGG 480

Query: 481 LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK 540
           LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK
Sbjct: 481 LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK 540

Query: 541 WEEKEVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIYDGMEVTLREPLVLDRSP 600
           W E+EVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWI +G+EVTLREPLVLDR+ 
Sbjct: 541 WAEREVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIDNGIEVTLREPLVLDRAL 600

Query: 601 FLERPGFWLPKAISNTIGNETKFDAQRRNSLEEESAKPIVQVST 645
           FLERPGFWL KAISNTIGNETK DAQRR S+EEESA+PIV+ ST
Sbjct: 601 FLERPGFWLSKAISNTIGNETKLDAQRRISVEEESAEPIVRAST 636

BLAST of HG10003735 vs. ExPASy Swiss-Prot
Match: Q8W4I6 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BPG2 PE=1 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 6.6e-223
Identity = 421/666 (63.21%), Postives = 513/666 (77.03%), Query Frame = 0

Query: 1   MAVLLSA----MELNLKLPHNNF-CHGLNIRTRPPFFPGLNRQETAHKSRMQPISLAVKS 60
           M VL+S+      +  KL   NF    L  R   PFF GL+  E   K  +  + LAVK 
Sbjct: 1   MVVLISSTVTICNVKPKLEDGNFRVSRLIHRPEVPFFSGLS-NEKKKKCAVSVMCLAVKK 60

Query: 61  QLTVHTTSTKIQG---KGRTKNPVLSEGRDEDEQNGD-ICPGCGVFMQDEDPNFLGFYQK 120
           +  V +  + + G     ++KN ++SEGRDEDE  G  ICPGCG+FMQD DP+  G+YQK
Sbjct: 61  EQVVQSVES-VNGTIFPKKSKNLIMSEGRDEDEDYGKIICPGCGIFMQDNDPDLPGYYQK 120

Query: 121 RKVSLTEAMEDGEDVE-DEFYG--VVDDDVVDEEENSDGQ--------------EIGDGF 180
           RKV +   +E  E VE DE  G  +VDDD  +EEE  D +              E   GF
Sbjct: 121 RKV-IANNLEGDEHVENDELAGFEMVDDDADEEEEGEDDEMDDEIKNAIEGSNSESESGF 180

Query: 181 DWDSDEWEAKLMKEEENDLELDGFAPADVGYGNITEETVKRAEKKRISKSEKKRRAREAQ 240
           +W+SDEWE    K+E ND+ELDGFAPA VGYGN+TEE   + +KKR+SK+E+K+ ARE  
Sbjct: 181 EWESDEWEE---KKEVNDVELDGFAPAGVGYGNVTEE---KEKKKRVSKTERKKIAREEA 240

Query: 241 KE--IEEVTVCARCHSLRNYGQVKNQAAENLIPDFDFDRLIANRLMKSTSNLN-NVVVMV 300
           K+   ++VTVCARCHSLRNYGQVKNQAAENL+PDFDFDRLI+ RL+K  SN +  VVVMV
Sbjct: 241 KKDNYDDVTVCARCHSLRNYGQVKNQAAENLLPDFDFDRLISTRLIKPMSNSSTTVVVMV 300

Query: 301 VDCVDFDGSFPKRAAKSLFKALEGTKNNPKMSKKLPKLVLVATKVDLLPSQISPTRLDRW 360
           VDCVDFDGSFPKRAAKSLF+ L+  +N+PK SK LPKLVLVATKVDLLP+QISP RLDRW
Sbjct: 301 VDCVDFDGSFPKRAAKSLFQVLQKAENDPKGSKNLPKLVLVATKVDLLPTQISPARLDRW 360

Query: 361 VRHRAKAAGAPKLTSVYLVSSRKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLINA 420
           VRHRAKA GAPKL+ VY+VS+RKD+GV+NLL++IKELAGPRGNVWVIGAQNAGKSTLINA
Sbjct: 361 VRHRAKAGGAPKLSGVYMVSARKDIGVKNLLAYIKELAGPRGNVWVIGAQNAGKSTLINA 420

Query: 421 LAKKERAKVTKLTEAPIPGTTLGILRIAGILSAQAKLFDTPGLLHPYLVSMRLNREEQKM 480
           L+KK+ AKVT+LTEAP+PGTTLGIL+I GILSA+AK++DTPGLLHPYL+S+RLN EE+KM
Sbjct: 421 LSKKDGAKVTRLTEAPVPGTTLGILKIGGILSAKAKMYDTPGLLHPYLMSLRLNSEERKM 480

Query: 481 VEIRKELQPRTYRVKASCAFMRQAVHVGGLVRLDLNQASVETIYVTVWASPNVSLHLGKI 540
           VEIRKE+QPR+YRVKA      Q+VH+GGLVRLDL  ASVETIY+T+WAS +VSLHLGK 
Sbjct: 481 VEIRKEVQPRSYRVKAG-----QSVHIGGLVRLDLVSASVETIYITIWASHSVSLHLGKT 540

Query: 541 ENADEIWKKHAGIRLQPPIGVDRASEIGKWEEKEVKISGTSWVVNSIDISIAGLGWFSLG 600
           ENA+EI+K H+G+RLQPPIG +RASE+G WEEKE+++SG SW V SIDIS+AGLGW SLG
Sbjct: 541 ENAEEIFKGHSGLRLQPPIGENRASELGTWEEKEIQVSGNSWDVKSIDISVAGLGWLSLG 600

Query: 601 LKGEATLTLWIYDGMEVTLREPLVLDRSPFLERPGFWLPKAISNTIGNETK--FDAQRRN 636
           LKG ATL LW Y G++VTLREPLV+DR+P+LERPGFWLPKAI+  +G  +    DA+RR 
Sbjct: 601 LKGAATLALWTYQGIDVTLREPLVIDRAPYLERPGFWLPKAITEVLGTHSSKLVDARRRK 652

BLAST of HG10003735 vs. ExPASy Swiss-Prot
Match: P54453 (Uncharacterized protein YqeH OS=Bacillus subtilis (strain 168) OX=224308 GN=yqeH PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 3.2e-36
Identity = 110/378 (29.10%), Postives = 192/378 (50.79%), Query Frame = 0

Query: 218 EEVTVCARCHSLRNYGQVKNQAAENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDF 277
           +E  +C RC  L+NY ++++ +    + D DF       ++      +++VV +VD  DF
Sbjct: 31  KENVICQRCFRLKNYNEIQDVS----LTDDDF-----LNILHGIGETDSLVVKIVDIFDF 90

Query: 278 DGSFPKRAAKSLFKALEGTKNNPKMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAK 337
           +GS+       L + + G   NP        ++LV  K D+LP  +   RL +W++  AK
Sbjct: 91  NGSW----INGLQRLVGG---NP--------ILLVGNKADILPKSLKRERLIQWMKREAK 150

Query: 338 AAGAPKLTSVYLVSSRKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKER 397
             G  K   V+LVS+ +  G+R ++  I+     + +V+V+G  N GKST IN + K+  
Sbjct: 151 ELGL-KPVDVFLVSAGRGQGIREVIDAIEHYRNGK-DVYVVGCTNVGKSTFINRIIKEVS 210

Query: 398 AKVTKLTEAPIPGTTLGILRIAGILSAQAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKE 457
            +   +T +  PGTTL  + I   L   + L+DTPG+++ + ++  +N+++ K++  +KE
Sbjct: 211 GEEDIITTSQFPGTTLDAIEIP--LDDGSSLYDTPGIINNHQMAHYVNKKDLKILSPKKE 270

Query: 458 LQPRTYRVKASCAFMRQAVHVGGLVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEI 517
           L+PRT+++       +Q ++ GGL R D    S E      +    + +H  K+ENAD +
Sbjct: 271 LKPRTFQLND-----QQTLYFGGLARFD--YVSGERSPFICYMPNELMIHRTKLENADAL 330

Query: 518 WKKHAGIRLQPPIGVDRASEIGKWEEKEVKISGTSWVVNSIDISIAGLGWFSLGLKGEAT 577
           ++KHAG  L PP G D   E  +       I          DI  +GLGW ++    +  
Sbjct: 331 YEKHAGELLTPP-GKDEMDEFPELVAHTFTIKD-----KKTDIVFSGLGWVTVH-DADKK 366

Query: 578 LTLWIYDGMEVTLREPLV 596
           +T +   G+ V +R  L+
Sbjct: 391 VTAYAPKGVHVFVRRSLI 366

BLAST of HG10003735 vs. ExPASy Swiss-Prot
Match: Q66GP9 (NO-associated protein 1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NOA1 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 4.5e-22
Identity = 105/373 (28.15%), Postives = 184/373 (49.33%), Query Frame = 0

Query: 222 VCARCHSLRNYGQVKNQAAENLIPDFDFDRLIANRLMKSTSNLNN---VVVMVVDCVDFD 281
           +C RC  L ++G +      N         + A+ L +  S+L +   ++V +VD VDF+
Sbjct: 144 ICGRC-QLLSHGHMITAVGGNGGYPGGKQFVSADELREKLSHLRHEKALIVKLVDIVDFN 203

Query: 282 GSFPKRAAKSLFKALEGTKNNPKMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKA 341
           GSF  R      + L G   NP        ++LV TK+DLLP       +  WV    + 
Sbjct: 204 GSFLARV-----RDLVGA--NP--------IILVITKIDLLPKGTDMNCIGDWV---VEV 263

Query: 342 AGAPKLT--SVYLVSSRKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAK-- 401
               KL   SV+L SS+   GV  + S I++    R +V+++GA N GKS  INAL K  
Sbjct: 264 TMRKKLNVLSVHLTSSKSLDGVSGVASEIQKEKKGR-DVYILGAANVGKSAFINALLKTM 323

Query: 402 KER------AKVTKLTEAPIPGTTLGILRIAGILSAQAKLFDTPGLLHPYLVSMRLNREE 461
            ER      A+  K  ++ +PGTTLG ++I   +  + KL+DTPG+   +  +  ++ ++
Sbjct: 324 AERDPVAAAAQKYKPIQSAVPGTTLGPIQINAFVGGE-KLYDTPGVHLHHRQAAVVHSDD 383

Query: 462 QKMVEIRKELQPRTYRV-----KASCAFMRQAVH-----VGGLVRLDLNQASVETIYVTV 521
              +  +  L+ +++ +     ++S +   ++++      GGLVR+D+ +A  ET + T 
Sbjct: 384 LPALAPQNRLRGQSFDISTLPTQSSSSPKGESLNGYTFFWGGLVRIDILKALPETCF-TF 443

Query: 522 WASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASE-IGKWEEKEVKISGTSWVVNS 571
           +    + +H    + A   ++K  G+ L PP G ++  E  G    + ++I        +
Sbjct: 444 YGPKALEIHAVPTKTATAFYEKELGVLLTPPSGKNQMQEWKGLQSHRLLQIEINDAKRPA 494

BLAST of HG10003735 vs. ExPASy Swiss-Prot
Match: Q9JJG9 (Nitric oxide-associated protein 1 OS=Mus musculus OX=10090 GN=Noa1 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.5e-09
Identity = 71/352 (20.17%), Postives = 133/352 (37.78%), Query Frame = 0

Query: 347 VYLVSSRKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAK------KERAKV 406
           V L+S++   GV  ++S ++     RG+V+++G  NAGKSTL N L +      K    +
Sbjct: 315 VRLISAKTGYGVEEMISALQRSWRYRGDVYLVGTTNAGKSTLFNTLLESDYCTAKGSEAI 374

Query: 407 TKLTEAPIPGTTLGILR------------------------------------------- 466
            + T +P PGTTL +L+                                           
Sbjct: 375 DRATISPWPGTTLNLLKFPICNPTPYRMFKRQRRLQEDATKAEEDLSEEEQSQLNQLKKH 434

Query: 467 -------------------------------------IAGILSAQAKL------------ 526
                                                +  +  +  ++            
Sbjct: 435 GYIVGRVGRTFSYSREQDEVPFEFDADSLAFDMGSEPVVSVCKSTKQIELTPEDVKDAHW 494

Query: 527 -FDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKASCAFMRQAVHVGGLVRLDLN 586
            +DTPG+     +   L  +E   V     + PRT+ +K         + +GG+ R+D  
Sbjct: 495 FYDTPGITKESCILNLLTEKEINTVLPTHSIIPRTFVLKPG-----MVLFLGGIARIDFL 554

Query: 587 QASVETIYVTVWASPNVSLHLGKIENADEIWKKHAG--IRLQPPIGVDRASEIGKWEEKE 596
           Q + ++ + TV AS  + +H+  ++ AD +++KHAG  + L P  G +R ++      ++
Sbjct: 555 QGN-QSAWFTVVASNFLPVHITSLDKADALYEKHAGHELLLVPMGGKERMAQFPPLVAED 614

BLAST of HG10003735 vs. ExPASy Swiss-Prot
Match: Q32LB9 (Nitric oxide-associated protein 1 OS=Bos taurus OX=9913 GN=NOA1 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 5.7e-09
Identity = 79/359 (22.01%), Postives = 137/359 (38.16%), Query Frame = 0

Query: 344 LTSVYLVSSRKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAK------KER 403
           L  V L+S++   GV  L+S ++     RG+V+++G+ NAGKSTL N L +      K  
Sbjct: 311 LRDVRLISAKTGYGVEELISALQRSWRYRGDVYLVGSTNAGKSTLFNTLLESDYCIAKGA 370

Query: 404 AKVTKLTEAPIPGTTLGILRI--------------------------------------- 463
             + + T +P PGTTL +L+                                        
Sbjct: 371 EAIDRATISPWPGTTLNLLKFPICNPTPYRMFQRQKRLKRDATEAEEDLSQQEQNKLNLL 430

Query: 464 ---------------------------------------AGILSAQAK------------ 523
                                                    +L+A               
Sbjct: 431 KKHGYLVGRVGRTFSYSEAQKDKAAFEFDADSFAFDMGNEPVLAADKSTKRVELTPEDVK 490

Query: 524 ----LFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKASCAFMRQAVHVGGLVR 583
                +DTPG+     +   L  +E  +V     + PRT+ +K         + +G L R
Sbjct: 491 DAHWFYDTPGITKENCILSLLTEKEVNIVLPTHSIIPRTFMLKPG-----MVLFLGALGR 550

Query: 584 LDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRL--QPPIGVDRASEIGKW 596
           +D  + + ++ + TV AS  + +H+  ++ AD +++KHAG  L   P  G +R +     
Sbjct: 551 IDFLKGN-QSAWFTVVASNFLPVHITSLDKADTMYQKHAGHTLLKVPVGGEERMAGFPPL 610

BLAST of HG10003735 vs. ExPASy TrEMBL
Match: A0A0A0KB28 (G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G044000 PE=4 SV=1)

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 584/653 (89.43%), Postives = 607/653 (92.96%), Query Frame = 0

Query: 1   MAVLLSAMELNLKLPHNNFCHGLNIRTRPPFFPGLNRQETAHKSRMQPISLAVKSQLTVH 60
           MA+LLSAMELNLKLP NNF HGLNIRTRPPF PG NRQ TAH+ + QPISLAVKSQ  V 
Sbjct: 1   MAILLSAMELNLKLPVNNFYHGLNIRTRPPFLPGPNRQVTAHRIQFQPISLAVKSQQIVR 60

Query: 61  TTSTKIQGKGRTKNPVLSEGRDEDEQNGDICPGCGVFMQDEDPNFLGFYQKRKVSLTEAM 120
           TTS+KIQGKGRTK+ VLSEGRDEDEQNGDICPGCGVFMQDEDPN LGFYQKRKVSLTE M
Sbjct: 61  TTSSKIQGKGRTKDSVLSEGRDEDEQNGDICPGCGVFMQDEDPNVLGFYQKRKVSLTEPM 120

Query: 121 EDGEDVEDEFYGVVD---------DDVVDEEENSDGQEIGDGFDWDSDEWEAKLMKEEEN 180
           EDGEDVEDEFYG+VD          DVVDEEENSD +EI DGFDWDSDEWEAKLM++EEN
Sbjct: 121 EDGEDVEDEFYGIVDSDVVDEEENSDVVDEEENSDAEEIEDGFDWDSDEWEAKLMEDEEN 180

Query: 181 DLELDGFAPADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRN 240
           +LELDGFAPADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRN
Sbjct: 181 NLELDGFAPADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRN 240

Query: 241 YGQVKNQAAENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFK 300
           YGQVKNQAAENLIPDFDFDRL+ANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFK
Sbjct: 241 YGQVKNQAAENLIPDFDFDRLMANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFK 300

Query: 301 ALEGTKNNPKMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTSVYLVS 360
           ALEG KN+PKM KKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKL  VYLVS
Sbjct: 301 ALEGNKNDPKMGKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLAGVYLVS 360

Query: 361 SRKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGT 420
           SRKDVGV+NLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGT
Sbjct: 361 SRKDVGVKNLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGT 420

Query: 421 TLGILRIAGILSAQAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKASCAF 480
           TLGILRI GILSA+AKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVK     
Sbjct: 421 TLGILRIGGILSAKAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKVG--- 480

Query: 481 MRQAVHVGGLVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIG 540
             Q VHVGGLVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIG
Sbjct: 481 --QTVHVGGLVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIG 540

Query: 541 VDRASEIGKWEEKEVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIYDGMEVTLR 600
           VDRASEIGKW E+EVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWI +G+EVT+R
Sbjct: 541 VDRASEIGKWAEREVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIDNGIEVTMR 600

Query: 601 EPLVLDRSPFLERPGFWLPKAISNTIGNETKFDAQRRNSLEEESAKPIVQVST 645
           EPLVLDR+ F+ERPGFWL KAISNTIGNETK DAQRR S+EEESA+P V+ ST
Sbjct: 601 EPLVLDRATFIERPGFWLSKAISNTIGNETKLDAQRRISVEEESAEPFVRAST 648

BLAST of HG10003735 vs. ExPASy TrEMBL
Match: A0A5D3DEP6 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold482G00530 PE=4 SV=1)

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 582/644 (90.37%), Postives = 606/644 (94.10%), Query Frame = 0

Query: 1   MAVLLSAMELNLKLPHNNFCHGLNIRTRPPFFPGLNRQETAHKSRMQPISLAVKSQLTVH 60
           MA+LLSAMELNLKLP NNF  GLN+RTRPPFFPG NR  TAHK + QPISLAVKSQ  V 
Sbjct: 1   MAILLSAMELNLKLPDNNFYRGLNVRTRPPFFPGPNRLGTAHKIQFQPISLAVKSQQIVR 60

Query: 61  TTSTKIQGKGRTKNPVLSEGRDEDEQNGDICPGCGVFMQDEDPNFLGFYQKRKVSLTEAM 120
            TS+KIQGKGRTK+ VLSEGRDEDEQNGDICPGCGVFMQDEDPN LGFYQKRKVSL+E M
Sbjct: 61  ATSSKIQGKGRTKDSVLSEGRDEDEQNGDICPGCGVFMQDEDPNLLGFYQKRKVSLSEPM 120

Query: 121 EDGEDVEDEFYGVVDDDVVDEEENSDGQEIGDGFDWDSDEWEAKLMKEEENDLELDGFAP 180
           ED EDVEDEFYG++D DVVDEEENSDG+EI D FDWD+DEWEAKLM E+ENDLELDGFAP
Sbjct: 121 EDVEDVEDEFYGILDSDVVDEEENSDGEEIEDDFDWDTDEWEAKLM-EDENDLELDGFAP 180

Query: 181 ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA 240
           ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA
Sbjct: 181 ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA 240

Query: 241 ENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEGTKNNP 300
           ENLIPDFDFDRL+ANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEG KN+P
Sbjct: 241 ENLIPDFDFDRLMANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEGNKNDP 300

Query: 301 KMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTSVYLVSSRKDVGVRN 360
           K+SKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLT VYLVSSRKDVGVRN
Sbjct: 301 KLSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTGVYLVSSRKDVGVRN 360

Query: 361 LLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG 420
           LLS IK+LAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG
Sbjct: 361 LLSLIKQLAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG 420

Query: 421 ILSAQAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKASCAFMRQAVHVGG 480
           ILSA+AKLFDTPGLLHPYL+SMRLNREEQKMVEIRKELQPRTYRVK       Q VHVGG
Sbjct: 421 ILSAKAKLFDTPGLLHPYLMSMRLNREEQKMVEIRKELQPRTYRVKVG-----QTVHVGG 480

Query: 481 LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK 540
           LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK
Sbjct: 481 LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK 540

Query: 541 WEEKEVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIYDGMEVTLREPLVLDRSP 600
           W E+EVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWI +G+EVTLREPLVLDR+ 
Sbjct: 541 WAEREVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIDNGIEVTLREPLVLDRAL 600

Query: 601 FLERPGFWLPKAISNTIGNETKFDAQRRNSLEEESAKPIVQVST 645
           FLERPGFWL KAISNTIGNETK DAQRR S+EEESA+PIV+ ST
Sbjct: 601 FLERPGFWLSKAISNTIGNETKLDAQRRISVEEESAEPIVRAST 638

BLAST of HG10003735 vs. ExPASy TrEMBL
Match: A0A1S3BEB4 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103488692 PE=4 SV=1)

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 582/644 (90.37%), Postives = 606/644 (94.10%), Query Frame = 0

Query: 1   MAVLLSAMELNLKLPHNNFCHGLNIRTRPPFFPGLNRQETAHKSRMQPISLAVKSQLTVH 60
           MA+LLSAMELNLKLP NNF  GLN+RTRPPFFPG NR  TAHK + QPISLAVKSQ  V 
Sbjct: 1   MAILLSAMELNLKLPDNNFYRGLNVRTRPPFFPGPNRLGTAHKIQFQPISLAVKSQQIVR 60

Query: 61  TTSTKIQGKGRTKNPVLSEGRDEDEQNGDICPGCGVFMQDEDPNFLGFYQKRKVSLTEAM 120
            TS+KIQGKGRTK+ VLSEGRDEDEQNGDICPGCGVFMQDEDPN LGFYQKRKVSL+E M
Sbjct: 61  ATSSKIQGKGRTKDSVLSEGRDEDEQNGDICPGCGVFMQDEDPNLLGFYQKRKVSLSEPM 120

Query: 121 EDGEDVEDEFYGVVDDDVVDEEENSDGQEIGDGFDWDSDEWEAKLMKEEENDLELDGFAP 180
           ED EDVEDEFYG++D DVVDEEENSDG+EI D FDWD+DEWEAKLM E+ENDLELDGFAP
Sbjct: 121 EDVEDVEDEFYGILDSDVVDEEENSDGEEIEDDFDWDTDEWEAKLM-EDENDLELDGFAP 180

Query: 181 ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA 240
           ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA
Sbjct: 181 ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA 240

Query: 241 ENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEGTKNNP 300
           ENLIPDFDFDRL+ANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEG KN+P
Sbjct: 241 ENLIPDFDFDRLMANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEGNKNDP 300

Query: 301 KMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTSVYLVSSRKDVGVRN 360
           K+SKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLT VYLVSSRKDVGVRN
Sbjct: 301 KLSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTGVYLVSSRKDVGVRN 360

Query: 361 LLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG 420
           LLS IK+LAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG
Sbjct: 361 LLSLIKQLAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG 420

Query: 421 ILSAQAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKASCAFMRQAVHVGG 480
           ILSA+AKLFDTPGLLHPYL+SMRLNREEQKMVEIRKELQPRTYRVK       Q VHVGG
Sbjct: 421 ILSAKAKLFDTPGLLHPYLMSMRLNREEQKMVEIRKELQPRTYRVKVG-----QTVHVGG 480

Query: 481 LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK 540
           LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK
Sbjct: 481 LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK 540

Query: 541 WEEKEVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIYDGMEVTLREPLVLDRSP 600
           W E+EVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWI +G+EVTLREPLVLDR+ 
Sbjct: 541 WAEREVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIDNGIEVTLREPLVLDRAL 600

Query: 601 FLERPGFWLPKAISNTIGNETKFDAQRRNSLEEESAKPIVQVST 645
           FLERPGFWL KAISNTIGNETK DAQRR S+EEESA+PIV+ ST
Sbjct: 601 FLERPGFWLSKAISNTIGNETKLDAQRRISVEEESAEPIVRAST 638

BLAST of HG10003735 vs. ExPASy TrEMBL
Match: A0A5A7SUS0 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold269G00320 PE=4 SV=1)

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 581/644 (90.22%), Postives = 605/644 (93.94%), Query Frame = 0

Query: 1   MAVLLSAMELNLKLPHNNFCHGLNIRTRPPFFPGLNRQETAHKSRMQPISLAVKSQLTVH 60
           MA+LLSAMELNLKLP NNF  GLN+RTRPPFFPG NR  TAHK + QPISLAVKSQ  V 
Sbjct: 1   MAILLSAMELNLKLPDNNFYRGLNVRTRPPFFPGPNRLGTAHKIQFQPISLAVKSQQIVR 60

Query: 61  TTSTKIQGKGRTKNPVLSEGRDEDEQNGDICPGCGVFMQDEDPNFLGFYQKRKVSLTEAM 120
            TS+KIQGKGRTK+ VLSEGRDEDEQNGDICPGCGVFMQDEDPN LGFYQKRKVSL+E M
Sbjct: 61  ATSSKIQGKGRTKDSVLSEGRDEDEQNGDICPGCGVFMQDEDPNLLGFYQKRKVSLSEPM 120

Query: 121 EDGEDVEDEFYGVVDDDVVDEEENSDGQEIGDGFDWDSDEWEAKLMKEEENDLELDGFAP 180
           ED EDVEDEFYG++D DVVDEEENSDG+EI D FDWD+DEWEAKLM E+ENDLELDGFAP
Sbjct: 121 EDVEDVEDEFYGILDSDVVDEEENSDGEEIEDDFDWDTDEWEAKLM-EDENDLELDGFAP 180

Query: 181 ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA 240
           ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA
Sbjct: 181 ADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNYGQVKNQAA 240

Query: 241 ENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEGTKNNP 300
           ENLIPDFDFDRL+ANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEG KN+P
Sbjct: 241 ENLIPDFDFDRLMANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKALEGNKNDP 300

Query: 301 KMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTSVYLVSSRKDVGVRN 360
           K+SKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLT VYLVSSRKDVGVRN
Sbjct: 301 KLSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTGVYLVSSRKDVGVRN 360

Query: 361 LLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG 420
           LLS IK+LAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG
Sbjct: 361 LLSLIKQLAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAG 420

Query: 421 ILSAQAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKASCAFMRQAVHVGG 480
           ILSA+AKLFDTPGLLHPYL+SMRLNREEQKMVEIRKELQPRTYRV        Q VHVGG
Sbjct: 421 ILSAKAKLFDTPGLLHPYLMSMRLNREEQKMVEIRKELQPRTYRV-------GQTVHVGG 480

Query: 481 LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK 540
           LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK
Sbjct: 481 LVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASEIGK 540

Query: 541 WEEKEVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIYDGMEVTLREPLVLDRSP 600
           W E+EVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWI +G+EVTLREPLVLDR+ 
Sbjct: 541 WAEREVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIDNGIEVTLREPLVLDRAL 600

Query: 601 FLERPGFWLPKAISNTIGNETKFDAQRRNSLEEESAKPIVQVST 645
           FLERPGFWL KAISNTIGNETK DAQRR S+EEESA+PIV+ ST
Sbjct: 601 FLERPGFWLSKAISNTIGNETKLDAQRRISVEEESAEPIVRAST 636

BLAST of HG10003735 vs. ExPASy TrEMBL
Match: A0A6J1L620 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499511 PE=4 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 7.9e-304
Identity = 537/645 (83.26%), Postives = 583/645 (90.39%), Query Frame = 0

Query: 1   MAVLLSAMELNLKLPHNNFCHGLNIRTRPPFFPGLNRQETAHKSRMQPISLAVKSQLTVH 60
           MA+ LSA  L L L HN+ C G  +RTRP  FPG  R+  AH++++    LAVKSQL+VH
Sbjct: 1   MAISLSATALTLNLAHNSICTGFRVRTRPRLFPGFIREGIAHENQIPVFRLAVKSQLSVH 60

Query: 61  TTSTKIQGKGRTKNPVLSEGRDEDEQNGDICPGCGVFMQDEDPNFLGFYQKRKVSLTEAM 120
           TTSTKIQGKGRTKNP+LSEGRDEDE+ GDICPGCGVFMQDEDPN  G+YQKRKVS+T   
Sbjct: 61  TTSTKIQGKGRTKNPLLSEGRDEDEKMGDICPGCGVFMQDEDPNVPGYYQKRKVSVT--- 120

Query: 121 EDGEDVEDEFYGVVDDDVVDEE--------ENSDGQEIGDGFDWDSDEWEAKLMKEEEND 180
              E +ED+FYG+++D+  DE+        E+SDG+EI DGFDWDSDEWEAK+M+EEE+D
Sbjct: 121 ---EPIEDDFYGIIEDEDGDEDEDDAMFEGEDSDGEEIVDGFDWDSDEWEAKIMEEEESD 180

Query: 181 LELDGFAPADVGYGNITEETVKRAEKKRISKSEKKRRAREAQKEIEEVTVCARCHSLRNY 240
           L+LDGFAPADVGYGNITEETVKRAEKKRISKSEKK+RAREA+KE EEVTVCARCHSLRNY
Sbjct: 181 LDLDGFAPADVGYGNITEETVKRAEKKRISKSEKKKRAREAEKEREEVTVCARCHSLRNY 240

Query: 241 GQVKNQAAENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKA 300
           GQVKNQAAENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKA
Sbjct: 241 GQVKNQAAENLIPDFDFDRLIANRLMKSTSNLNNVVVMVVDCVDFDGSFPKRAAKSLFKA 300

Query: 301 LEGTKNNPKMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLTSVYLVSS 360
           LEG KNNPK+SKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKL  VYLVS+
Sbjct: 301 LEGNKNNPKISKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKAAGAPKLAGVYLVSA 360

Query: 361 RKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAKKERAKVTKLTEAPIPGTT 420
           RKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLIN  AKKE AKVTKLTEAPIPGTT
Sbjct: 361 RKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLINTFAKKEGAKVTKLTEAPIPGTT 420

Query: 421 LGILRIAGILSAQAKLFDTPGLLHPYLVSMRLNREEQKMVEIRKELQPRTYRVKASCAFM 480
           LGILRIAGILSA+AKLFDTPGLLHPYL SMRL+REEQKMVEIRKELQPRTYRVK      
Sbjct: 421 LGILRIAGILSAKAKLFDTPGLLHPYLASMRLDREEQKMVEIRKELQPRTYRVKVG---- 480

Query: 481 RQAVHVGGLVRLDLNQASVETIYVTVWASPNVSLHLGKIENADEIWKKHAGIRLQPPIGV 540
            QAVHVGGLVRLDL+QASV+TIYVTVWASPNVSLHLGKIENADEIWKKHAG+RLQPPIGV
Sbjct: 481 -QAVHVGGLVRLDLSQASVQTIYVTVWASPNVSLHLGKIENADEIWKKHAGVRLQPPIGV 540

Query: 541 DRASEIGKWEEKEVKISGTSWVVNSIDISIAGLGWFSLGLKGEATLTLWIYDGMEVTLRE 600
           DRASEIGKWEE+E+K+SG+SW VNSIDISIAGLGWFSLGLKGEATLTLWI +G+EV+LRE
Sbjct: 541 DRASEIGKWEEREIKVSGSSWDVNSIDISIAGLGWFSLGLKGEATLTLWIDNGIEVSLRE 600

Query: 601 PLVLDRSPFLERPGFWLPKAISNTIGNETKFDAQRRNSLEEESAK 638
           PLVLDR+ FLERPGFWLPKAISN+IGNETK DAQRR +LEEES +
Sbjct: 601 PLVLDRAAFLERPGFWLPKAISNSIGNETKLDAQRRKALEEESVE 634

BLAST of HG10003735 vs. TAIR 10
Match: AT3G57180.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 775.0 bits (2000), Expect = 4.7e-224
Identity = 421/666 (63.21%), Postives = 513/666 (77.03%), Query Frame = 0

Query: 1   MAVLLSA----MELNLKLPHNNF-CHGLNIRTRPPFFPGLNRQETAHKSRMQPISLAVKS 60
           M VL+S+      +  KL   NF    L  R   PFF GL+  E   K  +  + LAVK 
Sbjct: 1   MVVLISSTVTICNVKPKLEDGNFRVSRLIHRPEVPFFSGLS-NEKKKKCAVSVMCLAVKK 60

Query: 61  QLTVHTTSTKIQG---KGRTKNPVLSEGRDEDEQNGD-ICPGCGVFMQDEDPNFLGFYQK 120
           +  V +  + + G     ++KN ++SEGRDEDE  G  ICPGCG+FMQD DP+  G+YQK
Sbjct: 61  EQVVQSVES-VNGTIFPKKSKNLIMSEGRDEDEDYGKIICPGCGIFMQDNDPDLPGYYQK 120

Query: 121 RKVSLTEAMEDGEDVE-DEFYG--VVDDDVVDEEENSDGQ--------------EIGDGF 180
           RKV +   +E  E VE DE  G  +VDDD  +EEE  D +              E   GF
Sbjct: 121 RKV-IANNLEGDEHVENDELAGFEMVDDDADEEEEGEDDEMDDEIKNAIEGSNSESESGF 180

Query: 181 DWDSDEWEAKLMKEEENDLELDGFAPADVGYGNITEETVKRAEKKRISKSEKKRRAREAQ 240
           +W+SDEWE    K+E ND+ELDGFAPA VGYGN+TEE   + +KKR+SK+E+K+ ARE  
Sbjct: 181 EWESDEWEE---KKEVNDVELDGFAPAGVGYGNVTEE---KEKKKRVSKTERKKIAREEA 240

Query: 241 KE--IEEVTVCARCHSLRNYGQVKNQAAENLIPDFDFDRLIANRLMKSTSNLN-NVVVMV 300
           K+   ++VTVCARCHSLRNYGQVKNQAAENL+PDFDFDRLI+ RL+K  SN +  VVVMV
Sbjct: 241 KKDNYDDVTVCARCHSLRNYGQVKNQAAENLLPDFDFDRLISTRLIKPMSNSSTTVVVMV 300

Query: 301 VDCVDFDGSFPKRAAKSLFKALEGTKNNPKMSKKLPKLVLVATKVDLLPSQISPTRLDRW 360
           VDCVDFDGSFPKRAAKSLF+ L+  +N+PK SK LPKLVLVATKVDLLP+QISP RLDRW
Sbjct: 301 VDCVDFDGSFPKRAAKSLFQVLQKAENDPKGSKNLPKLVLVATKVDLLPTQISPARLDRW 360

Query: 361 VRHRAKAAGAPKLTSVYLVSSRKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLINA 420
           VRHRAKA GAPKL+ VY+VS+RKD+GV+NLL++IKELAGPRGNVWVIGAQNAGKSTLINA
Sbjct: 361 VRHRAKAGGAPKLSGVYMVSARKDIGVKNLLAYIKELAGPRGNVWVIGAQNAGKSTLINA 420

Query: 421 LAKKERAKVTKLTEAPIPGTTLGILRIAGILSAQAKLFDTPGLLHPYLVSMRLNREEQKM 480
           L+KK+ AKVT+LTEAP+PGTTLGIL+I GILSA+AK++DTPGLLHPYL+S+RLN EE+KM
Sbjct: 421 LSKKDGAKVTRLTEAPVPGTTLGILKIGGILSAKAKMYDTPGLLHPYLMSLRLNSEERKM 480

Query: 481 VEIRKELQPRTYRVKASCAFMRQAVHVGGLVRLDLNQASVETIYVTVWASPNVSLHLGKI 540
           VEIRKE+QPR+YRVKA      Q+VH+GGLVRLDL  ASVETIY+T+WAS +VSLHLGK 
Sbjct: 481 VEIRKEVQPRSYRVKAG-----QSVHIGGLVRLDLVSASVETIYITIWASHSVSLHLGKT 540

Query: 541 ENADEIWKKHAGIRLQPPIGVDRASEIGKWEEKEVKISGTSWVVNSIDISIAGLGWFSLG 600
           ENA+EI+K H+G+RLQPPIG +RASE+G WEEKE+++SG SW V SIDIS+AGLGW SLG
Sbjct: 541 ENAEEIFKGHSGLRLQPPIGENRASELGTWEEKEIQVSGNSWDVKSIDISVAGLGWLSLG 600

Query: 601 LKGEATLTLWIYDGMEVTLREPLVLDRSPFLERPGFWLPKAISNTIGNETK--FDAQRRN 636
           LKG ATL LW Y G++VTLREPLV+DR+P+LERPGFWLPKAI+  +G  +    DA+RR 
Sbjct: 601 LKGAATLALWTYQGIDVTLREPLVIDRAPYLERPGFWLPKAITEVLGTHSSKLVDARRRK 652

BLAST of HG10003735 vs. TAIR 10
Match: AT4G10620.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 430.3 bits (1105), Expect = 2.8e-120
Identity = 234/533 (43.90%), Postives = 325/533 (60.98%), Query Frame = 0

Query: 90  ICPGCGVFMQDEDPNFLGFYQKRKVSLTEAMEDGEDVEDEFYGVVDDDVVDEEENSDGQE 149
           +CPGCGV MQ+ +P   GF+ K                                      
Sbjct: 85  VCPGCGVHMQNSNPKHPGFFIKPST----------------------------------- 144

Query: 150 IGDGFDWDSDEWEAKLMKEEENDLELDGFAPADVGYGNITEETVKRA-EKKRISKSEKKR 209
                            +++ NDL L    P       I  +++KR    + IS S+   
Sbjct: 145 -----------------EKQRNDLNLRDLTPISQEPEFI--DSIKRGFIIEPISSSD--L 204

Query: 210 RAREAQKEIEEVTVCARCHSLRNYGQVKNQAAENLIPDFDFDRLIANRLMKSTSNLNNVV 269
             R+ +       VCARCHSLR+YG+VK+   ENL+PDFDFD  +  RL  S S    VV
Sbjct: 205 NPRDDEPSDSRPLVCARCHSLRHYGRVKDPTVENLLPDFDFDHTVGRRL-GSASGARTVV 264

Query: 270 VMVVDCVDFDGSFPKRAAKSLFKALEGTKNNPKMSK--KLPKLVLVATKVDLLPSQISPT 329
           +MVVD  DFDGSFPKR AK + + ++      K  K   +P++V+V TK+DLLPS +SP 
Sbjct: 265 LMVVDASDFDGSFPKRVAKLVSRTIDENNMAWKEGKSGNVPRVVVVVTKIDLLPSSLSPN 324

Query: 330 RLDRWVRHRAKAAGAPKLTSVYLVSSRKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKS 389
           R ++WVR RA+  G  K+T ++ VS  K+ G+++L+  +  +AG RG+VW +G+QNAGKS
Sbjct: 325 RFEQWVRLRAREGGLSKITKLHFVSPVKNWGIKDLVEDVAAMAGKRGHVWAVGSQNAGKS 384

Query: 390 TLINALAKKERAKVTKLTEAPIPGTTLGILRIAGILSAQAKLFDTPGLLHPYLVSMRLNR 449
           TLINA+ K    KV  LTEAP+PGTTLGI+RI G+L  +AKLFDTPGLL+P+ ++ RL R
Sbjct: 385 TLINAVGKVVGGKVWHLTEAPVPGTTLGIIRIEGVLPFEAKLFDTPGLLNPHQITTRLTR 444

Query: 450 EEQKMVEIRKELQPRTYRVKASCAFMRQAVHVGGLVRLDLNQASVETIYVTVWASPNVSL 509
           EEQ++V I KEL+PRTYR+K         VH+GGL+RLD+++ASV+++YVTVWASP V L
Sbjct: 445 EEQRLVHISKELKPRTYRIKEG-----YTVHIGGLMRLDIDEASVDSLYVTVWASPYVPL 504

Query: 510 HLGKIENADEIWKKHAGIRLQPPIGVDRASEIGKWEEKEVKISGTSWVVNSIDISIAGLG 569
           H+GK ENA +  + H G RLQPPIG  R  E+GKW  KE ++SGTSW  +S+DI+++GLG
Sbjct: 505 HMGKKENAYKTLEDHFGCRLQPPIGEKRVEELGKWVRKEFRVSGTSWDTSSVDIAVSGLG 555

Query: 570 WFSLGLKGEATLTLWIYDGMEVTLREPLVLDRSPFLERPGFWLPKAISNTIGN 620
           WF+LGLKG+A L +W ++G++V  R+ L+  R+   E  GF + K ++    N
Sbjct: 565 WFALGLKGDAILGVWTHEGIDVFCRDSLLPQRAHTFEDSGFTVSKIVAKADRN 555

BLAST of HG10003735 vs. TAIR 10
Match: AT3G47450.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 107.8 bits (268), Expect = 3.2e-23
Identity = 105/373 (28.15%), Postives = 184/373 (49.33%), Query Frame = 0

Query: 222 VCARCHSLRNYGQVKNQAAENLIPDFDFDRLIANRLMKSTSNLNN---VVVMVVDCVDFD 281
           +C RC  L ++G +      N         + A+ L +  S+L +   ++V +VD VDF+
Sbjct: 144 ICGRC-QLLSHGHMITAVGGNGGYPGGKQFVSADELREKLSHLRHEKALIVKLVDIVDFN 203

Query: 282 GSFPKRAAKSLFKALEGTKNNPKMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKA 341
           GSF  R      + L G   NP        ++LV TK+DLLP       +  WV    + 
Sbjct: 204 GSFLARV-----RDLVGA--NP--------IILVITKIDLLPKGTDMNCIGDWV---VEV 263

Query: 342 AGAPKLT--SVYLVSSRKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAK-- 401
               KL   SV+L SS+   GV  + S I++    R +V+++GA N GKS  INAL K  
Sbjct: 264 TMRKKLNVLSVHLTSSKSLDGVSGVASEIQKEKKGR-DVYILGAANVGKSAFINALLKTM 323

Query: 402 KER------AKVTKLTEAPIPGTTLGILRIAGILSAQAKLFDTPGLLHPYLVSMRLNREE 461
            ER      A+  K  ++ +PGTTLG ++I   +  + KL+DTPG+   +  +  ++ ++
Sbjct: 324 AERDPVAAAAQKYKPIQSAVPGTTLGPIQINAFVGGE-KLYDTPGVHLHHRQAAVVHSDD 383

Query: 462 QKMVEIRKELQPRTYRV-----KASCAFMRQAVH-----VGGLVRLDLNQASVETIYVTV 521
              +  +  L+ +++ +     ++S +   ++++      GGLVR+D+ +A  ET + T 
Sbjct: 384 LPALAPQNRLRGQSFDISTLPTQSSSSPKGESLNGYTFFWGGLVRIDILKALPETCF-TF 443

Query: 522 WASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASE-IGKWEEKEVKISGTSWVVNS 571
           +    + +H    + A   ++K  G+ L PP G ++  E  G    + ++I        +
Sbjct: 444 YGPKALEIHAVPTKTATAFYEKELGVLLTPPSGKNQMQEWKGLQSHRLLQIEINDAKRPA 494

BLAST of HG10003735 vs. TAIR 10
Match: AT3G47450.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 105.1 bits (261), Expect = 2.1e-22
Identity = 104/373 (27.88%), Postives = 183/373 (49.06%), Query Frame = 0

Query: 222 VCARCHSLRNYGQVKNQAAENLIPDFDFDRLIANRLMKSTSNLNN---VVVMVVDCVDFD 281
           +C RC  L ++G +      N         + A+ L +  S+L +   ++V +VD VDF+
Sbjct: 144 ICGRC-QLLSHGHMITAVGGNGGYPGGKQFVSADELREKLSHLRHEKALIVKLVDIVDFN 203

Query: 282 GSFPKRAAKSLFKALEGTKNNPKMSKKLPKLVLVATKVDLLPSQISPTRLDRWVRHRAKA 341
           GSF  R      + L G   NP        ++LV TK+DLLP       +  WV    + 
Sbjct: 204 GSFLARV-----RDLVGA--NP--------IILVITKIDLLPKGTDMNCIGDWV---VEV 263

Query: 342 AGAPKLT--SVYLVSSRKDVGVRNLLSFIKELAGPRGNVWVIGAQNAGKSTLINALAK-- 401
               KL   SV+L SS+   GV  + S I++    R +V+++GA N GKS  INAL K  
Sbjct: 264 TMRKKLNVLSVHLTSSKSLDGVSGVASEIQKEKKGR-DVYILGAANVGKSAFINALLKTM 323

Query: 402 KER------AKVTKLTEAPIPGTTLGILRIAGILSAQAKLFDTPGLLHPYLVSMRLNREE 461
            ER      A+  K  ++ +PGTTLG ++I   +  + KL+DTPG+   +  +  ++ ++
Sbjct: 324 AERDPVAAAAQKYKPIQSAVPGTTLGPIQINAFVGGE-KLYDTPGVHLHHRQAAVVHSDD 383

Query: 462 QKMVEIRKELQPRTYRV-----KASCAFMRQAVH-----VGGLVRLDLNQASVETIYVTV 521
              +  +  L+ +++ +     ++S +   ++++      GGLVR+D+ +A  ET + T 
Sbjct: 384 LPALAPQNRLRGQSFDISTLPTQSSSSPKGESLNGYTFFWGGLVRIDILKALPETCF-TF 443

Query: 522 WASPNVSLHLGKIENADEIWKKHAGIRLQPPIGVDRASE-IGKWEEKEVKISGTSWVVNS 571
           +    + +H    + A   ++   G+ L PP G ++  E  G    + ++I        +
Sbjct: 444 YGPKALEIHAVPTKTATAFYEAKLGVLLTPPSGKNQMQEWKGLQSHRLLQIEINDAKRPA 494

BLAST of HG10003735 vs. TAIR 10
Match: AT1G52980.1 (GTP-binding family protein )

HSP 1 Score: 47.0 bits (110), Expect = 6.7e-05
Identity = 50/177 (28.25%), Postives = 79/177 (44.63%), Query Frame = 0

Query: 265 NNVVVMVVDCVDFDGSFPKRAAKSLFKALEGTKNNPKMSKKLPKLVLVATKVDLLPSQIS 324
           ++V+V V+D  D  G+      K+L           K   K   ++L+  K DL+P+  +
Sbjct: 216 SDVIVQVIDARDPQGTRCHHLEKTL-----------KEHHKHKHMILLLNKCDLVPAWAT 275

Query: 325 PTRLDRWVRHRAKAAGAPKLTSVYLVSSRKDVGVRNLLSFIKELAGPRG-----NVWVIG 384
                 W+R  +K       T  +  S  K  G  +LLS +++ A  +      +V  +G
Sbjct: 276 ----KGWLRVLSKEYP----TLAFHASVNKSFGKGSLLSVLRQFARLKSDKQAISVGFVG 335

Query: 385 AQNAGKSTLINALAKKERAKVTKLTEAPIPGTTLGILRIAGILSAQAKLFDTPGLLH 437
             N GKS++IN L  K   KV     APIPG T     I   L+ +  L D PG+++
Sbjct: 336 YPNVGKSSVINTLRTKNVCKV-----APIPGETKVWQYIT--LTKRIFLIDCPGVVY 366

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038885390.10.0e+0092.03GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Beninc... [more]
KAE8646627.10.0e+0089.74hypothetical protein Csa_005632 [Cucumis sativus][more]
XP_011656565.10.0e+0089.43GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Cucumi... [more]
XP_008445763.10.0e+0090.37PREDICTED: GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplas... [more]
KAA0032955.10.0e+0090.22GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 [Cucumis melo var. mak... [more]
Match NameE-valueIdentityDescription
Q8W4I66.6e-22363.21GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Arab... [more]
P544533.2e-3629.10Uncharacterized protein YqeH OS=Bacillus subtilis (strain 168) OX=224308 GN=yqeH... [more]
Q66GP94.5e-2228.15NO-associated protein 1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... [more]
Q9JJG91.5e-0920.17Nitric oxide-associated protein 1 OS=Mus musculus OX=10090 GN=Noa1 PE=1 SV=1[more]
Q32LB95.7e-0922.01Nitric oxide-associated protein 1 OS=Bos taurus OX=9913 GN=NOA1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KB280.0e+0089.43G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G044000 PE=4 SV=1[more]
A0A5D3DEP60.0e+0090.37GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 OS=Cucumis melo var. m... [more]
A0A1S3BEB40.0e+0090.37GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucu... [more]
A0A5A7SUS00.0e+0090.22GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 OS=Cucumis melo var. m... [more]
A0A6J1L6207.9e-30483.26GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucu... [more]
Match NameE-valueIdentityDescription
AT3G57180.14.7e-22463.21P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT4G10620.12.8e-12043.90P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G47450.23.2e-2328.15P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G47450.12.1e-2227.88P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G52980.16.7e-0528.25GTP-binding family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 193..216
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 62..87
NoneNo IPR availablePANTHERPTHR46434GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIALcoord: 23..637
NoneNo IPR availablePANTHERPTHR46434:SF3GTP-BINDING PROTEIN BRASSINAZOLE INSENSITIVE PALE GREEN 2, CHLOROPLASTICcoord: 23..637
NoneNo IPR availableCDDcd01855YqeHcoord: 223..434
e-value: 3.60916E-53
score: 178.998
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 255..437
e-value: 1.2E-36
score: 128.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 261..456
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 375..450
e-value: 1.3E-11
score: 44.6
IPR030378Circularly permuted (CP)-type guanine nucleotide-binding (G) domainPROSITEPS51721G_CPcoord: 254..437
score: 34.808033

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10003735.1HG10003735.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009742 brassinosteroid mediated signaling pathway
biological_process GO:1901259 chloroplast rRNA processing
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005525 GTP binding