HG10003561 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10003561
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionAbhydrolase_3 domain-containing protein
LocationChr08: 3291544 .. 3292593 (-)
RNA-Seq ExpressionHG10003561
SyntenyHG10003561
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCATCTTCCATTTCTGGTAGTGAAATTTCAAGCTCTCAACTTCCATGGAAGCACCGTCTATTCCTTCGACTCGGTACTATACTCACCGACGTTTGTTGCCGATCCAATTTCACCGTTAACCGTTGGCTCACCGCAATCCTCGACCGGAAAATCCCCCCCTCTACCAAACCAATCGATGGCGTCTCCTCTTTCGACCTCACTATCGACACTTCCCGCAACCTTTGGGTTCGAATCTTCAATCCCACTATCCACGGTGAAGATTCGAATCTTCAATCTTTGCCGGTTATTTTCTATTACCACGGCGGCGGCTTCACCTTCTCTTACGCCGATTCAGCCCTCTCCCACATGTCCGCTCACCAATTCGCCAAACAATTGCCCGCCGTTGTAATCTCCGTCAACTACCGCCTCGCTCCGGAGTTCCGGTTCCCCTGCCAATACGACGACGGATTCGACGCCTTGAAATTCATCGACGAAACAGAGGAGGGAATCCTACCGGCAAGGGCGGATCTGAGCCGTTGCTTCGTTTTGGGGGAAAGCGCCGGAGGAAATCTAGGGCACCACGTGGCAGTGAGAGCAAGCGAGTACGAGTTCAAGAAAGTGAAAGTGAGTGGGTTTATAGCGTCACAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTGCGTAATCAGCCGCCGTTGAGTCTGAAAATGACGGATTGGTTCTGGAAGGCGTTTTTGCCGGAAGGGGAAGATCGAGATCACGCCGCGGCGAATGTGTTCGGGCCAAACGGAAGGGATGTTTCGGAGGTGGTGAAGTTTCCGGCGACGGTGGTTTTGGTGGGAGAGTTGGATTTGCTGCAGGATGGGCAGAGGAGGTATTATGAAGGGTTGAAGAGAATGGGGAAGGATGTGAAAATGGTGGAATTTCCAAATGCCATTCATGGATTCTTTGTATTCAAGGATCTGCCACAATATTCTTCCATGATTCAAGAGATGAAGGATTTCATTGCCACACAATTCCATAACTCTCCATTAATTGTTCTTGCTTCTCCCTAA

mRNA sequence

ATGTCATCTTCCATTTCTGGTAGTGAAATTTCAAGCTCTCAACTTCCATGGAAGCACCGTCTATTCCTTCGACTCGGTACTATACTCACCGACGTTTGTTGCCGATCCAATTTCACCGTTAACCGTTGGCTCACCGCAATCCTCGACCGGAAAATCCCCCCCTCTACCAAACCAATCGATGGCGTCTCCTCTTTCGACCTCACTATCGACACTTCCCGCAACCTTTGGGTTCGAATCTTCAATCCCACTATCCACGGTGAAGATTCGAATCTTCAATCTTTGCCGGTTATTTTCTATTACCACGGCGGCGGCTTCACCTTCTCTTACGCCGATTCAGCCCTCTCCCACATGTCCGCTCACCAATTCGCCAAACAATTGCCCGCCGTTGTAATCTCCGTCAACTACCGCCTCGCTCCGGAGTTCCGGTTCCCCTGCCAATACGACGACGGATTCGACGCCTTGAAATTCATCGACGAAACAGAGGAGGGAATCCTACCGGCAAGGGCGGATCTGAGCCGTTGCTTCGTTTTGGGGGAAAGCGCCGGAGGAAATCTAGGGCACCACGTGGCAGTGAGAGCAAGCGAGTACGAGTTCAAGAAAGTGAAAGTGAGTGGGTTTATAGCGTCACAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTGCGTAATCAGCCGCCGTTGAGTCTGAAAATGACGGATTGGTTCTGGAAGGCGTTTTTGCCGGAAGGGGAAGATCGAGATCACGCCGCGGCGAATGTGTTCGGGCCAAACGGAAGGGATGTTTCGGAGGTGGTGAAGTTTCCGGCGACGGTGGTTTTGGTGGGAGAGTTGGATTTGCTGCAGGATGGGCAGAGGAGGTATTATGAAGGGTTGAAGAGAATGGGGAAGGATGTGAAAATGGTGGAATTTCCAAATGCCATTCATGGATTCTTTGTATTCAAGGATCTGCCACAATATTCTTCCATGATTCAAGAGATGAAGGATTTCATTGCCACACAATTCCATAACTCTCCATTAATTGTTCTTGCTTCTCCCTAA

Coding sequence (CDS)

ATGTCATCTTCCATTTCTGGTAGTGAAATTTCAAGCTCTCAACTTCCATGGAAGCACCGTCTATTCCTTCGACTCGGTACTATACTCACCGACGTTTGTTGCCGATCCAATTTCACCGTTAACCGTTGGCTCACCGCAATCCTCGACCGGAAAATCCCCCCCTCTACCAAACCAATCGATGGCGTCTCCTCTTTCGACCTCACTATCGACACTTCCCGCAACCTTTGGGTTCGAATCTTCAATCCCACTATCCACGGTGAAGATTCGAATCTTCAATCTTTGCCGGTTATTTTCTATTACCACGGCGGCGGCTTCACCTTCTCTTACGCCGATTCAGCCCTCTCCCACATGTCCGCTCACCAATTCGCCAAACAATTGCCCGCCGTTGTAATCTCCGTCAACTACCGCCTCGCTCCGGAGTTCCGGTTCCCCTGCCAATACGACGACGGATTCGACGCCTTGAAATTCATCGACGAAACAGAGGAGGGAATCCTACCGGCAAGGGCGGATCTGAGCCGTTGCTTCGTTTTGGGGGAAAGCGCCGGAGGAAATCTAGGGCACCACGTGGCAGTGAGAGCAAGCGAGTACGAGTTCAAGAAAGTGAAAGTGAGTGGGTTTATAGCGTCACAGCCGTTTTTCGGCGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTGCGTAATCAGCCGCCGTTGAGTCTGAAAATGACGGATTGGTTCTGGAAGGCGTTTTTGCCGGAAGGGGAAGATCGAGATCACGCCGCGGCGAATGTGTTCGGGCCAAACGGAAGGGATGTTTCGGAGGTGGTGAAGTTTCCGGCGACGGTGGTTTTGGTGGGAGAGTTGGATTTGCTGCAGGATGGGCAGAGGAGGTATTATGAAGGGTTGAAGAGAATGGGGAAGGATGTGAAAATGGTGGAATTTCCAAATGCCATTCATGGATTCTTTGTATTCAAGGATCTGCCACAATATTCTTCCATGATTCAAGAGATGAAGGATTTCATTGCCACACAATTCCATAACTCTCCATTAATTGTTCTTGCTTCTCCCTAA

Protein sequence

MSSSISGSEISSSQLPWKHRLFLRLGTILTDVCCRSNFTVNRWLTAILDRKIPPSTKPIDGVSSFDLTIDTSRNLWVRIFNPTIHGEDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAHQFAKQLPAVVISVNYRLAPEFRFPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGESAGGNLGHHVAVRASEYEFKKVKVSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWKAFLPEGEDRDHAAANVFGPNGRDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKDVKMVEFPNAIHGFFVFKDLPQYSSMIQEMKDFIATQFHNSPLIVLASP
Homology
BLAST of HG10003561 vs. NCBI nr
Match: XP_038890362.1 (probable carboxylesterase 18 [Benincasa hispida])

HSP 1 Score: 629.8 bits (1623), Expect = 1.4e-176
Identity = 299/346 (86.42%), Postives = 323/346 (93.35%), Query Frame = 0

Query: 1   MSSSISGSEISSSQLPWKHRLFLRLGTILTDVCCRSNFTVNRWLTAILDRKIPPSTKPID 60
           MSSSIS  EISSS+LPWK+RL LRLGTI+TD CCRSNFTVNRWLT ILD KIPPSTKPID
Sbjct: 1   MSSSISACEISSSKLPWKYRLLLRLGTIITDACCRSNFTVNRWLTGILDWKIPPSTKPID 60

Query: 61  GVSSFDLTIDTSRNLWVRIFNPTIHGEDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAH 120
           GVSSFDLTIDTSRNLWVRIFNP I GE  NLQS+PVIFY+HGGGF FSYADSAL HM+AH
Sbjct: 61  GVSSFDLTIDTSRNLWVRIFNPIIDGEHPNLQSVPVIFYFHGGGFVFSYADSALFHMAAH 120

Query: 121 QFAKQLPAVVISVNYRLAPEFRFPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGES 180
           + AKQL AVVISVNYRLAPEFR PCQYDDGFDA+KFIDET+EGILP +ADLSRCF+LGES
Sbjct: 121 RLAKQLHAVVISVNYRLAPEFRCPCQYDDGFDAVKFIDETDEGILPEKADLSRCFILGES 180

Query: 181 AGGNLGHHVAVRASEYEFKKVKVSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFW 240
           AGGNLGHHVAVRASEY FKK+K+ GFIASQPFFGGEERTESE+RLRNQPPLSL++ DWFW
Sbjct: 181 AGGNLGHHVAVRASEYTFKKLKLIGFIASQPFFGGEERTESEMRLRNQPPLSLRLCDWFW 240

Query: 241 KAFLPEGEDRDHAAANVFGPNGRDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGK 300
           KAFLPEGE+RDHAAANVFGPNGRDVSEVVKFPATVV VGELDLLQDGQR+YYEGLKRMGK
Sbjct: 241 KAFLPEGENRDHAAANVFGPNGRDVSEVVKFPATVVFVGELDLLQDGQRKYYEGLKRMGK 300

Query: 301 DVKMVEFPNAIHGFFVFKDLPQYSSMIQEMKDFIATQFHNSPLIVL 347
           +VKMVEFPNAIHGFF F DLPQYSSMI+EMKDFIAT+FHNSP +++
Sbjct: 301 NVKMVEFPNAIHGFFAFWDLPQYSSMIKEMKDFIATEFHNSPPLIV 346

BLAST of HG10003561 vs. NCBI nr
Match: KAA0039342.1 (putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00525.1 putative carboxylesterase 18 [Cucumis melo var. makuwa])

HSP 1 Score: 614.4 bits (1583), Expect = 6.1e-172
Identity = 297/347 (85.59%), Postives = 318/347 (91.64%), Query Frame = 0

Query: 2   SSSISGSEISSSQLPWKHRLFLRLGTILTDVCCRSNFTVNRWLTAILDRKIPPSTKPIDG 61
           SSSIS  EISSS+LPWKHRL LR+GTI+TD CCRS+FTVNRWL+  LD KIPPSTKPIDG
Sbjct: 3   SSSISACEISSSKLPWKHRLLLRIGTIITDACCRSDFTVNRWLSGTLDWKIPPSTKPIDG 62

Query: 62  VSSFDLTIDTSRNLWVRIFNPTIHGEDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAHQ 121
           VSSFDLTIDTSRNLWVRIFNPTI  EDSNLQSLP+IFY+HGGGF FSYADSALSH SAH+
Sbjct: 63  VSSFDLTIDTSRNLWVRIFNPTIDDEDSNLQSLPLIFYFHGGGFAFSYADSALSHTSAHR 122

Query: 122 FAKQLPAVVISVNYRLAPEFRFPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGESA 181
           FAKQLPAVVISVNYRLAPEFR+PCQYDDGFDALKFIDE  E ILPA+ADLSRCF+LGESA
Sbjct: 123 FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLSRCFILGESA 182

Query: 182 GGNLGHHVAVRASEYEFKKVKVSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWK 241
           GGNLGHHVAVRASEY  KKV++ GFIASQPFFGGEERTESEIRLRNQ PL+L+++DWFWK
Sbjct: 183 GGNLGHHVAVRASEYTLKKVRLVGFIASQPFFGGEERTESEIRLRNQRPLNLRLSDWFWK 242

Query: 242 AFLPEGEDRDHAAANVFGPNGRDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKD 301
           AFLPEGEDRDHAAANVFGP GRDV EV+KFPAT+VLVGELDLLQDGQRRYYEGLKRMGK+
Sbjct: 243 AFLPEGEDRDHAAANVFGPKGRDVREVMKFPATLVLVGELDLLQDGQRRYYEGLKRMGKE 302

Query: 302 VKMVEFPNAIHGFFVFKDLPQYSSMIQEMKDFIATQFHNSPLIVLAS 349
           VKMVEF NAIHGFF F DLPQYSSMI+EMKDFIAT   NS  IV AS
Sbjct: 303 VKMVEFENAIHGFFAFWDLPQYSSMIKEMKDFIATHTQNS--IVFAS 347

BLAST of HG10003561 vs. NCBI nr
Match: XP_008459712.1 (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 612.1 bits (1577), Expect = 3.0e-171
Identity = 296/347 (85.30%), Postives = 317/347 (91.35%), Query Frame = 0

Query: 2   SSSISGSEISSSQLPWKHRLFLRLGTILTDVCCRSNFTVNRWLTAILDRKIPPSTKPIDG 61
           SSSIS  EISSS+LPWKHRL LR+GTI+TD CCRS+FTVNRWL+  LD KIPPSTKPIDG
Sbjct: 3   SSSISACEISSSKLPWKHRLLLRIGTIITDACCRSDFTVNRWLSGTLDWKIPPSTKPIDG 62

Query: 62  VSSFDLTIDTSRNLWVRIFNPTIHGEDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAHQ 121
           VSSFDLTIDTSRNLWVRIFNP I  EDSNLQSLP+IFY+HGGGF FSYADSALSH SAH+
Sbjct: 63  VSSFDLTIDTSRNLWVRIFNPIIDDEDSNLQSLPLIFYFHGGGFAFSYADSALSHTSAHR 122

Query: 122 FAKQLPAVVISVNYRLAPEFRFPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGESA 181
           FAKQLPAVVISVNYRLAPEFR+PCQYDDGFDALKFIDE  E ILPA+ADLSRCF+LGESA
Sbjct: 123 FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLSRCFILGESA 182

Query: 182 GGNLGHHVAVRASEYEFKKVKVSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWK 241
           GGNLGHHVAVRASEY  KKV++ GFIASQPFFGGEERTESEIRLRNQ PL+L+++DWFWK
Sbjct: 183 GGNLGHHVAVRASEYTLKKVRLVGFIASQPFFGGEERTESEIRLRNQRPLNLRLSDWFWK 242

Query: 242 AFLPEGEDRDHAAANVFGPNGRDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKD 301
           AFLPEGEDRDHAAANVFGP GRDV EV+KFPAT+VLVGELDLLQDGQRRYYEGLKRMGK+
Sbjct: 243 AFLPEGEDRDHAAANVFGPKGRDVREVMKFPATLVLVGELDLLQDGQRRYYEGLKRMGKE 302

Query: 302 VKMVEFPNAIHGFFVFKDLPQYSSMIQEMKDFIATQFHNSPLIVLAS 349
           VKMVEF NAIHGFF F DLPQYSSMI+EMKDFIAT   NS  IV AS
Sbjct: 303 VKMVEFENAIHGFFAFWDLPQYSSMIKEMKDFIATHTQNS--IVFAS 347

BLAST of HG10003561 vs. NCBI nr
Match: XP_004141616.1 (probable carboxylesterase 18 [Cucumis sativus] >KGN52614.1 hypothetical protein Csa_009173 [Cucumis sativus])

HSP 1 Score: 609.0 bits (1569), Expect = 2.6e-170
Identity = 292/348 (83.91%), Postives = 317/348 (91.09%), Query Frame = 0

Query: 1   MSSSISGSEISSSQLPWKHRLFLRLGTILTDVCCRSNFTVNRWLTAILDRKIPPSTKPID 60
           MSSS S SEISSS+LPWKHRL LR+G+ +TD CCRS+FTVNRW T ILD KIPPSTKPID
Sbjct: 1   MSSSTSASEISSSKLPWKHRLLLRIGSTITDACCRSDFTVNRWFTGILDWKIPPSTKPID 60

Query: 61  GVSSFDLTIDTSRNLWVRIFNPTIHGEDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAH 120
           GVSSFDLTIDTSRNLWVRIFNP I GEDS++QSLP+IFY+HGGGF FSYADSALSH SAH
Sbjct: 61  GVSSFDLTIDTSRNLWVRIFNPVIDGEDSDIQSLPLIFYFHGGGFAFSYADSALSHTSAH 120

Query: 121 QFAKQLPAVVISVNYRLAPEFRFPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGES 180
           +FAKQLPAVVISVNYRLAPEFR+PCQYDDGFDALKFIDE  E ILPA+ADL+RCF+LGES
Sbjct: 121 RFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLTRCFILGES 180

Query: 181 AGGNLGHHVAVRASEYEFKKVKVSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFW 240
           AGGNLGHHVAVRASEY  KKVK+ GFIASQPFFGGEERTESEIRL NQ PLSL+++DWFW
Sbjct: 181 AGGNLGHHVAVRASEYTLKKVKMVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFW 240

Query: 241 KAFLPEGEDRDHAAANVFGPNGRDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGK 300
           KAFLPEGEDRDH AANVFGP GRDV+EV+KFPAT+V+VGELDLLQDGQRRYYEGLKRMGK
Sbjct: 241 KAFLPEGEDRDHGAANVFGPKGRDVTEVMKFPATLVMVGELDLLQDGQRRYYEGLKRMGK 300

Query: 301 DVKMVEFPNAIHGFFVFKDLPQYSSMIQEMKDFIATQFHNSPLIVLAS 349
           +VKMVEF NAIHGFF F DLPQYSSM++EMKDFIAT   NS  IV AS
Sbjct: 301 EVKMVEFENAIHGFFAFWDLPQYSSMMKEMKDFIATHIQNS--IVSAS 346

BLAST of HG10003561 vs. NCBI nr
Match: XP_022973390.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 547.7 bits (1410), Expect = 7.1e-152
Identity = 262/330 (79.39%), Postives = 286/330 (86.67%), Query Frame = 0

Query: 11  SSSQLPWKHRLFLRLGTILTDVCCRSNFTVNRWLTAILDRKIPPSTKPIDGVSSFDLTID 70
           S+ +LPWKHRL LRLGTI T+  CRS+FT+NRWLT ILDR+ PPS+KPIDGVSS DL ID
Sbjct: 10  STLRLPWKHRLMLRLGTIATNTFCRSDFTINRWLTGILDRRTPPSSKPIDGVSSSDLLID 69

Query: 71  TSRNLWVRIFNPTIHGEDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAHQFAKQLPAVV 130
            SRNLWVRIF P       ++QSLPVIFYYHGGGF FSYADS+L H +AHQFAKQL AVV
Sbjct: 70  KSRNLWVRIFTPI-----DDVQSLPVIFYYHGGGFAFSYADSSLYHTTAHQFAKQLRAVV 129

Query: 131 ISVNYRLAPEFRFPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGESAGGNLGHHVA 190
           ISVNYRLAPEFRFPCQYDDGFDALKFIDE ++G LPA ADL R F+LGESAGGNLGHHVA
Sbjct: 130 ISVNYRLAPEFRFPCQYDDGFDALKFIDEIDDGTLPASADLGRSFILGESAGGNLGHHVA 189

Query: 191 VRASEYEFKKVKVSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWKAFLPEGEDR 250
           VRASEY FKK+K+ GFIA+QPF G EERTESEIRL NQPPLSLK+TDWFWKAFLP G DR
Sbjct: 190 VRASEYAFKKLKLIGFIATQPFIGAEERTESEIRLCNQPPLSLKLTDWFWKAFLPVGSDR 249

Query: 251 DHAAANVFGPNGRDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKDVKMVEFPNA 310
           DHAAANVFGPNGRD+S V KFPATVVLVG  DLLQDGQRRYYEGLKRMGK+VKMVE PNA
Sbjct: 250 DHAAANVFGPNGRDISGVEKFPATVVLVGGFDLLQDGQRRYYEGLKRMGKEVKMVELPNA 309

Query: 311 IHGFFVFKDLPQYSSMIQEMKDFIATQFHN 341
           IHGFF F DLP+YSSMIQ ++DFIA Q+ N
Sbjct: 310 IHGFFCFPDLPEYSSMIQAVRDFIAAQWQN 334

BLAST of HG10003561 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 8.0e-82
Identity = 152/323 (47.06%), Postives = 208/323 (64.40%), Query Frame = 0

Query: 15  LPWKHRLFLRLGTILTDVCCRSNFTVNRWLTAILDRKIPPSTKPIDGVSSFDLTIDTSRN 74
           LP K R+ L + + +TD   R + T+NR    + D + PP+ KP++ VS+ D  +D SR+
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LWVRIFNPTIHGEDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAHQFAKQLPAVVISVN 134
           LW R++ P + G+      +PV+ ++HGGGF F   ++        +FA++LPA VISVN
Sbjct: 73  LWFRLYTPHVSGD-----KIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVN 132

Query: 135 YRLAPEFRFPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGESAGGNLGHHVAV--- 194
           YRLAPE R+P QYDDGFDALK+I+E    ILPA ADLSRCF  G+SAGGN+ H+VA+   
Sbjct: 133 YRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRIC 192

Query: 195 RASEYEFKKVKVSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWKAFLPEGEDRD 254
           R     F  VK+ G I+ QPFFGGEERTE+E +L   P +S   TDW WKA    G +RD
Sbjct: 193 REPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRD 252

Query: 255 HAAANVFGPNGRDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKDVKMVEFPNAI 314
           H A NV GPN  D+S  + +P T+V+V   D L+D QR YYE LK  GK   ++E+PN  
Sbjct: 253 HEAVNVGGPNAVDISG-LDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMF 312

Query: 315 HGFFVFKDLPQYSSMIQEMKDFI 335
           H F++F +LP+   +I  +KDF+
Sbjct: 313 HAFYIFPELPEAGQLIMRIKDFV 326

BLAST of HG10003561 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.4e-62
Identity = 125/309 (40.45%), Postives = 187/309 (60.52%), Query Frame = 0

Query: 35  RSNFTVNRWLTAILDRKIPPSTKPIDGVSSFDLTIDTSRNLWVRIFNPTIHGEDSNLQSL 94
           R + T NR L   LDRK+P +  P++GV SFD+ ID   NL  R++ P   G   ++  L
Sbjct: 36  RPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGTSPSITDL 95

Query: 95  ---------PVIFYYHGGGFTFSYADSALSHMSAHQFAKQLPAVVISVNYRLAPEFRFPC 154
                    PVI ++HGG F  S A+SA+      +      AVV+SVNYR APE R+PC
Sbjct: 96  QNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPC 155

Query: 155 QYDDGFDALKFIDETEEGILPARADLSRCFVLGESAGGNLGHHVAVRASEYEFKKVKVSG 214
            YDDG+  LK+++ +   +   +    R F+ G+S+GGN+ H+VAVRA E    ++ V G
Sbjct: 156 AYDDGWAVLKWVN-SSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLG 215

Query: 215 FIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWKAFLPEGEDRDHAAANVFGPNGRDV 274
            I   P FGG ERTESE RL  +  ++++  DW+W+AFLPEGEDR+H A + FGP  + +
Sbjct: 216 NILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSL 275

Query: 275 SEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKDVKMVEFPNAIHGFFVFKDLPQYSS 334
            E + FP ++V+V  LDL+QD Q +Y EGLK+ G++VK++    A  GF++  +   + +
Sbjct: 276 -EGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHT 335

BLAST of HG10003561 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.3e-60
Identity = 128/319 (40.13%), Postives = 183/319 (57.37%), Query Frame = 0

Query: 35  RSNFTVNRWLTAILDRKIPPSTKPIDGVSSFDLTIDTSRNLWVRIFNPTIHGEDSN---- 94
           R++ T  R L   LDR++P + +P++GVSSFD  ID S  L VRI+     G+       
Sbjct: 36  RADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVGLEVRIYRAAAEGDAEEGAAA 95

Query: 95  --------------LQSLPVIFYYHGGGFTFSYADSALSHMSAHQFAKQLPAVVISVNYR 154
                          +  PVI ++HGG F  S A S +      +F K    VV+SVNYR
Sbjct: 96  VTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGVVVSVNYR 155

Query: 155 LAPEFRFPCQYDDGFDALKFIDETEEGILPARADL-SRCFVLGESAGGNLGHHVAVRASE 214
            APE R+PC YDDG+ ALK++    +  + +  D  +R F+ G+S+GGN+ HHVAVRA++
Sbjct: 156 RAPEHRYPCAYDDGWTALKWV--MSQPFMRSGGDAQARVFLSGDSSGGNIAHHVAVRAAD 215

Query: 215 YEFKKVKVSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWKAFLPEGEDRDHAAA 274
              + VKV G I     FGG ERTESE RL  +  ++L+  DW+WKA+LPE  DRDH A 
Sbjct: 216 ---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDADRDHPAC 275

Query: 275 NVFGPNGRDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKDVKMVEFPNAIHGFF 334
           N FGPNGR +   + F  ++++V  LDL  D Q  Y + L+  G  VK+V+  NA  GF+
Sbjct: 276 NPFGPNGRRLGG-LPFAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCENATVGFY 335

BLAST of HG10003561 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 7.3e-59
Identity = 124/327 (37.92%), Postives = 197/327 (60.24%), Query Frame = 0

Query: 35  RSNFTVNRWLTAILDRKIPPSTKPIDGVSSFDLTIDTSRNLWVRIFNP------TIHG-- 94
           R + + NR L   LDRK+P ++ P+DGV SFD  +D++ NL  RI+ P      T HG  
Sbjct: 36  RPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLHQTRHGTL 95

Query: 95  ----EDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAHQFAKQLPAVVISVNYRLAPEFR 154
                 S  + +PV+ ++HGG FT S A+SA+      +       VV+SV+YR +PE R
Sbjct: 96  ELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDYRRSPEHR 155

Query: 155 FPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGESAGGNLGHHVAVRASEYEFKKVK 214
           +PC YDDG++AL ++ ++   +   +      ++ G+S+GGN+ H+VAVRA+    + VK
Sbjct: 156 YPCAYDDGWNALNWV-KSRVWLQSGKDSNVYVYLAGDSSGGNIAHNVAVRATN---EGVK 215

Query: 215 VSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWKAFLPEGEDRDHAAANVFGPNG 274
           V G I   P FGG+ERT+SE  L  +  ++++  DW+W+A+LPEGEDRDH A N FGP G
Sbjct: 216 VLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPACNPFGPRG 275

Query: 275 RDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKDVKMVEFPNAIHGFFVFKDLPQ 334
           + + + V FP ++V+V  LDL+QD Q  Y +GLK+ G +V ++    A  GF+   +   
Sbjct: 276 QSL-KGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFYFLPNNDH 335

Query: 335 YSSMIQEMKDFI----ATQFHNSPLIV 346
           +  +++E+  F+     +Q  +SP+++
Sbjct: 336 FHCLMEELNKFVHSIEDSQSKSSPVLL 356

BLAST of HG10003561 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 9.5e-59
Identity = 121/311 (38.91%), Postives = 182/311 (58.52%), Query Frame = 0

Query: 35  RSNFTVNRWLTAILDRKIPPSTKPIDGVSSFDLTIDTSRNLWVRIFNPTIHGEDS----- 94
           R + T NR L   LDRK+  +  P+DGV SFD+ ID   NL  R++ P    ++      
Sbjct: 36  RPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQPPSIL 95

Query: 95  NLQS------LPVIFYYHGGGFTFSYADSALSHMSAHQFAKQLPAVVISVNYRLAPEFRF 154
           +L+       +PVI ++HGG F  S A+SA+      +       VV+SVNYR APE  +
Sbjct: 96  DLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPY 155

Query: 155 PCQYDDGFDALKFIDETEEGILPARADLSRCFVLGESAGGNLGHHVAVRASEYEFKKVKV 214
           PC YDDG+ AL +++ +   +   +      F+ G+S+GGN+ H+VA+RA E     + V
Sbjct: 156 PCAYDDGWIALNWVN-SRSWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE---SGIDV 215

Query: 215 SGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWKAFLPEGEDRDHAAANVFGPNGR 274
            G I   P FGG ERTESE  L  +  ++++  DW+WKAFLPEGEDR+H A N F P G+
Sbjct: 216 LGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGK 275

Query: 275 DVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKDVKMVEFPNAIHGFFVFKDLPQY 334
            + E V FP ++V+V  LDL++D Q  Y EGLK+ G++VK++    A  GF++  +   +
Sbjct: 276 SL-EGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLLPNNNHF 335

BLAST of HG10003561 vs. ExPASy TrEMBL
Match: A0A5D3BN57 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001350 PE=4 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 3.0e-172
Identity = 297/347 (85.59%), Postives = 318/347 (91.64%), Query Frame = 0

Query: 2   SSSISGSEISSSQLPWKHRLFLRLGTILTDVCCRSNFTVNRWLTAILDRKIPPSTKPIDG 61
           SSSIS  EISSS+LPWKHRL LR+GTI+TD CCRS+FTVNRWL+  LD KIPPSTKPIDG
Sbjct: 3   SSSISACEISSSKLPWKHRLLLRIGTIITDACCRSDFTVNRWLSGTLDWKIPPSTKPIDG 62

Query: 62  VSSFDLTIDTSRNLWVRIFNPTIHGEDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAHQ 121
           VSSFDLTIDTSRNLWVRIFNPTI  EDSNLQSLP+IFY+HGGGF FSYADSALSH SAH+
Sbjct: 63  VSSFDLTIDTSRNLWVRIFNPTIDDEDSNLQSLPLIFYFHGGGFAFSYADSALSHTSAHR 122

Query: 122 FAKQLPAVVISVNYRLAPEFRFPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGESA 181
           FAKQLPAVVISVNYRLAPEFR+PCQYDDGFDALKFIDE  E ILPA+ADLSRCF+LGESA
Sbjct: 123 FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLSRCFILGESA 182

Query: 182 GGNLGHHVAVRASEYEFKKVKVSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWK 241
           GGNLGHHVAVRASEY  KKV++ GFIASQPFFGGEERTESEIRLRNQ PL+L+++DWFWK
Sbjct: 183 GGNLGHHVAVRASEYTLKKVRLVGFIASQPFFGGEERTESEIRLRNQRPLNLRLSDWFWK 242

Query: 242 AFLPEGEDRDHAAANVFGPNGRDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKD 301
           AFLPEGEDRDHAAANVFGP GRDV EV+KFPAT+VLVGELDLLQDGQRRYYEGLKRMGK+
Sbjct: 243 AFLPEGEDRDHAAANVFGPKGRDVREVMKFPATLVLVGELDLLQDGQRRYYEGLKRMGKE 302

Query: 302 VKMVEFPNAIHGFFVFKDLPQYSSMIQEMKDFIATQFHNSPLIVLAS 349
           VKMVEF NAIHGFF F DLPQYSSMI+EMKDFIAT   NS  IV AS
Sbjct: 303 VKMVEFENAIHGFFAFWDLPQYSSMIKEMKDFIATHTQNS--IVFAS 347

BLAST of HG10003561 vs. ExPASy TrEMBL
Match: A0A1S3CAT6 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498754 PE=4 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.5e-171
Identity = 296/347 (85.30%), Postives = 317/347 (91.35%), Query Frame = 0

Query: 2   SSSISGSEISSSQLPWKHRLFLRLGTILTDVCCRSNFTVNRWLTAILDRKIPPSTKPIDG 61
           SSSIS  EISSS+LPWKHRL LR+GTI+TD CCRS+FTVNRWL+  LD KIPPSTKPIDG
Sbjct: 3   SSSISACEISSSKLPWKHRLLLRIGTIITDACCRSDFTVNRWLSGTLDWKIPPSTKPIDG 62

Query: 62  VSSFDLTIDTSRNLWVRIFNPTIHGEDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAHQ 121
           VSSFDLTIDTSRNLWVRIFNP I  EDSNLQSLP+IFY+HGGGF FSYADSALSH SAH+
Sbjct: 63  VSSFDLTIDTSRNLWVRIFNPIIDDEDSNLQSLPLIFYFHGGGFAFSYADSALSHTSAHR 122

Query: 122 FAKQLPAVVISVNYRLAPEFRFPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGESA 181
           FAKQLPAVVISVNYRLAPEFR+PCQYDDGFDALKFIDE  E ILPA+ADLSRCF+LGESA
Sbjct: 123 FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLSRCFILGESA 182

Query: 182 GGNLGHHVAVRASEYEFKKVKVSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWK 241
           GGNLGHHVAVRASEY  KKV++ GFIASQPFFGGEERTESEIRLRNQ PL+L+++DWFWK
Sbjct: 183 GGNLGHHVAVRASEYTLKKVRLVGFIASQPFFGGEERTESEIRLRNQRPLNLRLSDWFWK 242

Query: 242 AFLPEGEDRDHAAANVFGPNGRDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKD 301
           AFLPEGEDRDHAAANVFGP GRDV EV+KFPAT+VLVGELDLLQDGQRRYYEGLKRMGK+
Sbjct: 243 AFLPEGEDRDHAAANVFGPKGRDVREVMKFPATLVLVGELDLLQDGQRRYYEGLKRMGKE 302

Query: 302 VKMVEFPNAIHGFFVFKDLPQYSSMIQEMKDFIATQFHNSPLIVLAS 349
           VKMVEF NAIHGFF F DLPQYSSMI+EMKDFIAT   NS  IV AS
Sbjct: 303 VKMVEFENAIHGFFAFWDLPQYSSMIKEMKDFIATHTQNS--IVFAS 347

BLAST of HG10003561 vs. ExPASy TrEMBL
Match: A0A0A0KUP3 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G647370 PE=4 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.2e-170
Identity = 292/348 (83.91%), Postives = 317/348 (91.09%), Query Frame = 0

Query: 1   MSSSISGSEISSSQLPWKHRLFLRLGTILTDVCCRSNFTVNRWLTAILDRKIPPSTKPID 60
           MSSS S SEISSS+LPWKHRL LR+G+ +TD CCRS+FTVNRW T ILD KIPPSTKPID
Sbjct: 1   MSSSTSASEISSSKLPWKHRLLLRIGSTITDACCRSDFTVNRWFTGILDWKIPPSTKPID 60

Query: 61  GVSSFDLTIDTSRNLWVRIFNPTIHGEDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAH 120
           GVSSFDLTIDTSRNLWVRIFNP I GEDS++QSLP+IFY+HGGGF FSYADSALSH SAH
Sbjct: 61  GVSSFDLTIDTSRNLWVRIFNPVIDGEDSDIQSLPLIFYFHGGGFAFSYADSALSHTSAH 120

Query: 121 QFAKQLPAVVISVNYRLAPEFRFPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGES 180
           +FAKQLPAVVISVNYRLAPEFR+PCQYDDGFDALKFIDE  E ILPA+ADL+RCF+LGES
Sbjct: 121 RFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLTRCFILGES 180

Query: 181 AGGNLGHHVAVRASEYEFKKVKVSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFW 240
           AGGNLGHHVAVRASEY  KKVK+ GFIASQPFFGGEERTESEIRL NQ PLSL+++DWFW
Sbjct: 181 AGGNLGHHVAVRASEYTLKKVKMVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFW 240

Query: 241 KAFLPEGEDRDHAAANVFGPNGRDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGK 300
           KAFLPEGEDRDH AANVFGP GRDV+EV+KFPAT+V+VGELDLLQDGQRRYYEGLKRMGK
Sbjct: 241 KAFLPEGEDRDHGAANVFGPKGRDVTEVMKFPATLVMVGELDLLQDGQRRYYEGLKRMGK 300

Query: 301 DVKMVEFPNAIHGFFVFKDLPQYSSMIQEMKDFIATQFHNSPLIVLAS 349
           +VKMVEF NAIHGFF F DLPQYSSM++EMKDFIAT   NS  IV AS
Sbjct: 301 EVKMVEFENAIHGFFAFWDLPQYSSMMKEMKDFIATHIQNS--IVSAS 346

BLAST of HG10003561 vs. ExPASy TrEMBL
Match: A0A6J1IB87 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111471943 PE=4 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 3.4e-152
Identity = 262/330 (79.39%), Postives = 286/330 (86.67%), Query Frame = 0

Query: 11  SSSQLPWKHRLFLRLGTILTDVCCRSNFTVNRWLTAILDRKIPPSTKPIDGVSSFDLTID 70
           S+ +LPWKHRL LRLGTI T+  CRS+FT+NRWLT ILDR+ PPS+KPIDGVSS DL ID
Sbjct: 10  STLRLPWKHRLMLRLGTIATNTFCRSDFTINRWLTGILDRRTPPSSKPIDGVSSSDLLID 69

Query: 71  TSRNLWVRIFNPTIHGEDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAHQFAKQLPAVV 130
            SRNLWVRIF P       ++QSLPVIFYYHGGGF FSYADS+L H +AHQFAKQL AVV
Sbjct: 70  KSRNLWVRIFTPI-----DDVQSLPVIFYYHGGGFAFSYADSSLYHTTAHQFAKQLRAVV 129

Query: 131 ISVNYRLAPEFRFPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGESAGGNLGHHVA 190
           ISVNYRLAPEFRFPCQYDDGFDALKFIDE ++G LPA ADL R F+LGESAGGNLGHHVA
Sbjct: 130 ISVNYRLAPEFRFPCQYDDGFDALKFIDEIDDGTLPASADLGRSFILGESAGGNLGHHVA 189

Query: 191 VRASEYEFKKVKVSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWKAFLPEGEDR 250
           VRASEY FKK+K+ GFIA+QPF G EERTESEIRL NQPPLSLK+TDWFWKAFLP G DR
Sbjct: 190 VRASEYAFKKLKLIGFIATQPFIGAEERTESEIRLCNQPPLSLKLTDWFWKAFLPVGSDR 249

Query: 251 DHAAANVFGPNGRDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKDVKMVEFPNA 310
           DHAAANVFGPNGRD+S V KFPATVVLVG  DLLQDGQRRYYEGLKRMGK+VKMVE PNA
Sbjct: 250 DHAAANVFGPNGRDISGVEKFPATVVLVGGFDLLQDGQRRYYEGLKRMGKEVKMVELPNA 309

Query: 311 IHGFFVFKDLPQYSSMIQEMKDFIATQFHN 341
           IHGFF F DLP+YSSMIQ ++DFIA Q+ N
Sbjct: 310 IHGFFCFPDLPEYSSMIQAVRDFIAAQWQN 334

BLAST of HG10003561 vs. ExPASy TrEMBL
Match: A0A6J1EWT7 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111438887 PE=4 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 3.2e-150
Identity = 259/330 (78.48%), Postives = 285/330 (86.36%), Query Frame = 0

Query: 11  SSSQLPWKHRLFLRLGTILTDVCCRSNFTVNRWLTAILDRKIPPSTKPIDGVSSFDLTID 70
           S+ +LPWKHRL LRLGTI T+  CRS+FT+NRWLT ILDR+ PPS+ PIDGVSS DL ID
Sbjct: 10  STLRLPWKHRLMLRLGTIATNTFCRSDFTINRWLTGILDRRTPPSSIPIDGVSSSDLLID 69

Query: 71  TSRNLWVRIFNPTIHGEDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAHQFAKQLPAVV 130
            SRNLWVRIF P        +QSLPVIFYYHGGGF FSYADS+L H +AHQFAKQL AVV
Sbjct: 70  PSRNLWVRIFTPI-----DAVQSLPVIFYYHGGGFAFSYADSSLYHTTAHQFAKQLRAVV 129

Query: 131 ISVNYRLAPEFRFPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGESAGGNLGHHVA 190
           ISVNYRLAPEFRFPCQYDDGFDALKFIDE ++G LPA ADL R F+LGESAGGNLGHHVA
Sbjct: 130 ISVNYRLAPEFRFPCQYDDGFDALKFIDEIDDGTLPASADLGRSFILGESAGGNLGHHVA 189

Query: 191 VRASEYEFKKVKVSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWKAFLPEGEDR 250
           VRASEY FKK+K+ GFIA+QPFFG EERTESEIRL NQ PLSL+++DWFWKAFLP G DR
Sbjct: 190 VRASEYAFKKLKLIGFIATQPFFGAEERTESEIRLCNQAPLSLRLSDWFWKAFLPVGSDR 249

Query: 251 DHAAANVFGPNGRDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKDVKMVEFPNA 310
           DHAAANVFGPNGRD+S V +FPATVVLVG  DLLQDGQRRYYEGLKRMGK+VKMVE PNA
Sbjct: 250 DHAAANVFGPNGRDISGVERFPATVVLVGGFDLLQDGQRRYYEGLKRMGKEVKMVELPNA 309

Query: 311 IHGFFVFKDLPQYSSMIQEMKDFIATQFHN 341
           IHGFF F DLP+YSSMIQE++DFIA Q+ N
Sbjct: 310 IHGFFCFPDLPEYSSMIQEVRDFIAAQWQN 334

BLAST of HG10003561 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 305.4 bits (781), Expect = 5.7e-83
Identity = 152/323 (47.06%), Postives = 208/323 (64.40%), Query Frame = 0

Query: 15  LPWKHRLFLRLGTILTDVCCRSNFTVNRWLTAILDRKIPPSTKPIDGVSSFDLTIDTSRN 74
           LP K R+ L + + +TD   R + T+NR    + D + PP+ KP++ VS+ D  +D SR+
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LWVRIFNPTIHGEDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAHQFAKQLPAVVISVN 134
           LW R++ P + G+      +PV+ ++HGGGF F   ++        +FA++LPA VISVN
Sbjct: 73  LWFRLYTPHVSGD-----KIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVN 132

Query: 135 YRLAPEFRFPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGESAGGNLGHHVAV--- 194
           YRLAPE R+P QYDDGFDALK+I+E    ILPA ADLSRCF  G+SAGGN+ H+VA+   
Sbjct: 133 YRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRIC 192

Query: 195 RASEYEFKKVKVSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWKAFLPEGEDRD 254
           R     F  VK+ G I+ QPFFGGEERTE+E +L   P +S   TDW WKA    G +RD
Sbjct: 193 REPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRD 252

Query: 255 HAAANVFGPNGRDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKDVKMVEFPNAI 314
           H A NV GPN  D+S  + +P T+V+V   D L+D QR YYE LK  GK   ++E+PN  
Sbjct: 253 HEAVNVGGPNAVDISG-LDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMF 312

Query: 315 HGFFVFKDLPQYSSMIQEMKDFI 335
           H F++F +LP+   +I  +KDF+
Sbjct: 313 HAFYIFPELPEAGQLIMRIKDFV 326

BLAST of HG10003561 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 241.5 bits (615), Expect = 1.0e-63
Identity = 125/309 (40.45%), Postives = 187/309 (60.52%), Query Frame = 0

Query: 35  RSNFTVNRWLTAILDRKIPPSTKPIDGVSSFDLTIDTSRNLWVRIFNPTIHGEDSNLQSL 94
           R + T NR L   LDRK+P +  P++GV SFD+ ID   NL  R++ P   G   ++  L
Sbjct: 36  RPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGTSPSITDL 95

Query: 95  ---------PVIFYYHGGGFTFSYADSALSHMSAHQFAKQLPAVVISVNYRLAPEFRFPC 154
                    PVI ++HGG F  S A+SA+      +      AVV+SVNYR APE R+PC
Sbjct: 96  QNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPC 155

Query: 155 QYDDGFDALKFIDETEEGILPARADLSRCFVLGESAGGNLGHHVAVRASEYEFKKVKVSG 214
            YDDG+  LK+++ +   +   +    R F+ G+S+GGN+ H+VAVRA E    ++ V G
Sbjct: 156 AYDDGWAVLKWVN-SSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLG 215

Query: 215 FIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWKAFLPEGEDRDHAAANVFGPNGRDV 274
            I   P FGG ERTESE RL  +  ++++  DW+W+AFLPEGEDR+H A + FGP  + +
Sbjct: 216 NILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSL 275

Query: 275 SEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKDVKMVEFPNAIHGFFVFKDLPQYSS 334
            E + FP ++V+V  LDL+QD Q +Y EGLK+ G++VK++    A  GF++  +   + +
Sbjct: 276 -EGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHT 335

BLAST of HG10003561 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 229.2 bits (583), Expect = 5.2e-60
Identity = 124/327 (37.92%), Postives = 197/327 (60.24%), Query Frame = 0

Query: 35  RSNFTVNRWLTAILDRKIPPSTKPIDGVSSFDLTIDTSRNLWVRIFNP------TIHG-- 94
           R + + NR L   LDRK+P ++ P+DGV SFD  +D++ NL  RI+ P      T HG  
Sbjct: 36  RPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLHQTRHGTL 95

Query: 95  ----EDSNLQSLPVIFYYHGGGFTFSYADSALSHMSAHQFAKQLPAVVISVNYRLAPEFR 154
                 S  + +PV+ ++HGG FT S A+SA+      +       VV+SV+YR +PE R
Sbjct: 96  ELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDYRRSPEHR 155

Query: 155 FPCQYDDGFDALKFIDETEEGILPARADLSRCFVLGESAGGNLGHHVAVRASEYEFKKVK 214
           +PC YDDG++AL ++ ++   +   +      ++ G+S+GGN+ H+VAVRA+    + VK
Sbjct: 156 YPCAYDDGWNALNWV-KSRVWLQSGKDSNVYVYLAGDSSGGNIAHNVAVRATN---EGVK 215

Query: 215 VSGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWKAFLPEGEDRDHAAANVFGPNG 274
           V G I   P FGG+ERT+SE  L  +  ++++  DW+W+A+LPEGEDRDH A N FGP G
Sbjct: 216 VLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPACNPFGPRG 275

Query: 275 RDVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKDVKMVEFPNAIHGFFVFKDLPQ 334
           + + + V FP ++V+V  LDL+QD Q  Y +GLK+ G +V ++    A  GF+   +   
Sbjct: 276 QSL-KGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFYFLPNNDH 335

Query: 335 YSSMIQEMKDFI----ATQFHNSPLIV 346
           +  +++E+  F+     +Q  +SP+++
Sbjct: 336 FHCLMEELNKFVHSIEDSQSKSSPVLL 356

BLAST of HG10003561 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 228.8 bits (582), Expect = 6.8e-60
Identity = 121/311 (38.91%), Postives = 182/311 (58.52%), Query Frame = 0

Query: 35  RSNFTVNRWLTAILDRKIPPSTKPIDGVSSFDLTIDTSRNLWVRIFNPTIHGEDS----- 94
           R + T NR L   LDRK+  +  P+DGV SFD+ ID   NL  R++ P    ++      
Sbjct: 36  RPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQPPSIL 95

Query: 95  NLQS------LPVIFYYHGGGFTFSYADSALSHMSAHQFAKQLPAVVISVNYRLAPEFRF 154
           +L+       +PVI ++HGG F  S A+SA+      +       VV+SVNYR APE  +
Sbjct: 96  DLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPY 155

Query: 155 PCQYDDGFDALKFIDETEEGILPARADLSRCFVLGESAGGNLGHHVAVRASEYEFKKVKV 214
           PC YDDG+ AL +++ +   +   +      F+ G+S+GGN+ H+VA+RA E     + V
Sbjct: 156 PCAYDDGWIALNWVN-SRSWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE---SGIDV 215

Query: 215 SGFIASQPFFGGEERTESEIRLRNQPPLSLKMTDWFWKAFLPEGEDRDHAAANVFGPNGR 274
            G I   P FGG ERTESE  L  +  ++++  DW+WKAFLPEGEDR+H A N F P G+
Sbjct: 216 LGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGK 275

Query: 275 DVSEVVKFPATVVLVGELDLLQDGQRRYYEGLKRMGKDVKMVEFPNAIHGFFVFKDLPQY 334
            + E V FP ++V+V  LDL++D Q  Y EGLK+ G++VK++    A  GF++  +   +
Sbjct: 276 SL-EGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLLPNNNHF 335

BLAST of HG10003561 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 181.0 bits (458), Expect = 1.6e-45
Identity = 100/287 (34.84%), Postives = 160/287 (55.75%), Query Frame = 0

Query: 54  PSTKPIDGVSSFDLTIDTSRNLWVRIFNPTIHGEDSNL--QSLPVIFYYHGGGFTFSYAD 113
           P   P++   S DL ++  ++ W+R++ P+    + N+  Q LP++ YYHGGGF     D
Sbjct: 38  PDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQKLPIVVYYHGGGFILCSVD 97

Query: 114 SALSHMSAHQFAKQLPAVVISVNYRLAPEFRFPCQYDDGFDALKFIDETEEGILPARADL 173
             L H    + A+ L A+V+S +YRLAPE R P  YDDG +AL +I  +++  + + AD 
Sbjct: 98  MQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVEALDWIKTSDDEWIKSHADF 157

Query: 174 SRCFVLGESAGGNLGHHVAVRA--SEYEFKKVKVSGFIASQPFFGGEERTESEIRLRNQP 233
           S  F++G SAGGNL ++V +R+  S  +   +++ G I   PFFGGEER+ESEIRL N  
Sbjct: 158 SNVFLMGTSAGGNLAYNVGLRSVDSVSDLSPLQIRGLILHHPFFGGEERSESEIRLMNDQ 217

Query: 234 PLSLKMTDWFWKAFLPEGEDRDHAAAN-VFGPNGRDVSEVVKFPATVVLV-GELDLLQDG 293
                +TD  W   LP G DRDH  +N   G     + ++ +    V+++ GE D + D 
Sbjct: 218 VCPPIVTDVMWDLSLPVGVDRDHEYSNPTVGDGSEKLEKIGRLRWKVMMIGGEDDPMIDL 277

Query: 294 QRRYYEGLKRMGKDVKMVEFPNAIHGFFVFKDLPQYSSMIQEMKDFI 335
           Q+   + +K+ G +V        +HG  + +D  +  ++   +K+FI
Sbjct: 278 QKDVAKLMKKKGVEVVEHYTGGHVHGAEI-RDPSKRKTLFLSIKNFI 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890362.11.4e-17686.42probable carboxylesterase 18 [Benincasa hispida][more]
KAA0039342.16.1e-17285.59putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00525.1 putative car... [more]
XP_008459712.13.0e-17185.30PREDICTED: probable carboxylesterase 18 [Cucumis melo][more]
XP_004141616.12.6e-17083.91probable carboxylesterase 18 [Cucumis sativus] >KGN52614.1 hypothetical protein ... [more]
XP_022973390.17.1e-15279.39probable carboxylesterase 18 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LT108.0e-8247.06Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q940G61.4e-6240.45Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q6L5452.3e-6040.13Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Q9LYC17.3e-5937.92Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Q9MAA79.5e-5938.91Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BN573.0e-17285.59Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CAT61.5e-17185.30probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498754 PE=4 SV=1[more]
A0A0A0KUP31.2e-17083.91Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6473... [more]
A0A6J1IB873.4e-15279.39probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111471943 PE=4 SV... [more]
A0A6J1EWT73.2e-15078.48probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111438887 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT5G23530.15.7e-8347.06carboxyesterase 18 [more]
AT5G27320.11.0e-6340.45alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.15.2e-6037.92alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.16.8e-6038.91alpha/beta-Hydrolases superfamily protein [more]
AT5G62180.11.6e-4534.84carboxyesterase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 97..315
e-value: 7.3E-53
score: 179.6
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 4..342
e-value: 1.9E-88
score: 299.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 37..336
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 10..337
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 10..337

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10003561.1HG10003561.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity