HG10003554 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10003554
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionDNA primase
LocationChr08: 3173894 .. 3178607 (-)
RNA-Seq ExpressionHG10003554
SyntenyHG10003554
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTACTGCAGCGGTTGAGAATGGCGGAAGCGAAATGGTAATTGACGGTAACGAACAGAAGCCGAAGAACATTGTGTCTGATGGGTTCGATGCCAATTATCTCAAAATTTACTACGGTGAGCCATTTATTCCTTTTCGATTTTTTTTGAAACTTGTGTTGAATTGCTCCATCAAATTTAGTTTACTTATCTCTTCTATGTTTTTCTGCTACTGAAAGGATATAAAATGTTAATTCTTGCAGGGAAGCTTTTTCCGTATGCTGATTTTTTCAAATGGATGTCTTATGGAAATGGTAAGCCCTTGATTCTGGGAATCAGAAACAAAATATTATTCCCCAGAGGAAGCTAACATATATGTAACCTTCCTGCTTTATGTACTGGTAGATGGCAAGCATCCGGGCAGTGACCAATCCTATTTTGGCCGAAGAGAGTTTTCATTCACCTTGGATAATGATATTTATCTGCGATTCCAATCTTTCAACAGTCTATCAGAAATGGAAAATTCAATCAAGGAAAAATGTCCATTTAAAATTGATATTGGGCCTGTATACAGCGTGGATGTAAGAAGAATTTTTCCCGGTTGCTCTTATCATTTTAAGGCTATCTTTCTAGTTTTCTCATGATTCCTGATGATTTAATTATTAAACTTTTGCATAGTTTGGTTTTATGTAGTCAGATTTACTTAAGTCAAGGCAACAAATAGTTTGCATGATTGTTTTGAGTCTTATTTGTTTGAAAGAGTTCTTTTTTGTGAGATTCATTTGTCTCTCTCACACACTGCAACTTTTCTACTTCAGCCTGCAAAGAGACATGCATATGCTGGCGATAATGTATTTACTCCAGTGGAGAGAGAGCTGGTTTTTGATATCGTAAGCAGACTGTTTTTTATGATGCTCAATGTTCTAATTCAATTTGAAAGTTAATTATGAGGTCCTTTTCATTGAGAAATTAATCGGAAATTTCCTCCACATGCAGGATATGACCGATTACGATGATGTTAGATATTGTTGTTCTGGAGCTGATGTTTGCTTGGATTGCTGGCCTTTAATGACAGCTGCTATCAAAGTGATTGATACTACCCTCCGAGGTAAGAAGATTTGTCATGTGTATATAAAAAATCGTGCAAGAAATGAGTATGATTGCATCCATTCTCCCAACTCTTAATTTGGAAAGGAATGAGCACAGAGAAGGTTGCATATTATTCAATGCTAGCACGTTTGAGAGAAATCGCTTACCTTCTTGGTAGTTTCCCCCTCCCGATCCCATGCCTAAGCCTATTTCTTTTTTCACTTTTGTTGGGAATGAGATATTCCATATTCCATATTGGGCTCTTAAGTGTACATACTTCTTTTTAACTTTTTCCTCAAGGCTTACTTCAAACATATTATTTTATTTAAAGAGAAGTAGGAAATACGACATGCATAAAAGGTGTTATACATTCTGAAAAGTTAAGATTATTAATCACTGGCTTATTGTCAGTTCTGTTCTGCAGTACTACTTTATCTACTGGAGCCAGTTATATGTGTAATGCTTATCTTTATTTAAGTATGTCCATACATTGAGCCTCTCTCACAAATATTTTGAAGAGATATTCTAAATTAACTATAATGGCATTGTGACATTGTCCAGTGCATCTGAGAATTAAGTTTTCAATTGTAGTCCTTTGATTAGATAGCACACGGTGTTCTGTTCACTCCCATCAGATACACTTCACTTAAAATTTGTGTTGCAGATGACTTTGGATTTAACCACATTCTATGGGTATTTAGTGGTCGGCGTGGTGTTCATTGTTGGGTTTGTGATGGCAAAGCTAGAAGGTAAAGCTAGTAATCAAATTGTTGAATTTGCAGTAAAATATATTATTTGTAAAGGTCTCAAATTCCCACCATCACTGCCACAGGTTGTCTAACGAACAGCGAGCTGCCATTGCTGAATATTTCCATTTATACCAGGTTATACTGTGGTGTCATAAAATGTTGGGGAATTTTAGTGTCTTTAGCTGTGGAGTTGAATATGGTTGATGTGCAGGGTAATGAAAATAGTCGCAAGAAAATTTCTCTAACGGGTCCAGTTCTTCATCCATTTCTTGCGTAAGTGGTGCTTAATATTAAGATATGATGATTCAAGTCTCATTTTTTTTCTCATCCAACTTATCTATTTTGTTTCAGGAGATCGTACATAGAAGTTCTCAAGGATAGCTTTGAATCACAATTGCTCTATAGTCAAAAGATTTTCTCTACTGAGGAAAGATATGAGAAAATATTAGATATGATTCATGATGAATGTAAGTGATTTTATGTATTTGTCTCAGGTAAGTACTGTCAGTATGATAATCTTTAGATACTAATAGAGTTACTCTTGCAGCCATTACCTCAGAGCTTCGAGGAAGGTGGCAAGATAATAGGCGACCCTCTAAAAATGATGTCAACTCAGTAAGATGGGAACAACTGAAAAATATGTTGCAATCAGGGAAACATAAGGTAGAATCAACATCTTTTCTCATACTTGCAAGAAGTTACAACTTGGAATAGATCATTGATGAGGAGTTTGTGTTGTAATGTTGCAGTTTGGAACCATTTTTGTCAAGTGTGAATGGTACCGAAAATAGAGATAGAAATGTTGTACTGAGCTTTTTCCCTTTTGTTTATGCCTTCTTTTTTCCAACTCAACTTACCAAACAGGGGAGGGATAGCCATTATACATGGTATTCTCCTCAAGGAAGCAAACGTAAATTTTGAGCTTGAGGAAGATGAAGAAGATGAAGAAGCTACTATTTTTTAAAGGACTTTACTGAGCTAGAGCTACTTTATGTTCTAAGGACTTCACTTAACAGTCAAAAGCTCCCTCTTCCCCTCCTGAAGACCGTTCACCTTCTTACTTTTTGTCCTTCAATGATAACTTCTTACACCTTTTGTCTTCATATGCTAAGTTCTTAAAAGCTGTAACCGATACCCTGTACCCTCTCCCCAAAAATCCAGCCTAATTATCAAAGCAGCTTTCTGCCCTAGGTCTCACATTCACCTTTCTTCTGGTAAAGGCCTCCAATCAACACCTTTGTCCAATAAACAAAATCATGTACCCCATTCTTTACCAAAGATTAAAAAAGAGTTTGTTTCTTTCAACTCCAGAATTTCAATGAATTTTCTATGCAAATAGCGCGAACACTTCACCTCCATGGACACTCATCCTTGAACCATACTTTGATAGACCTCTCAAGCCCTTTCTAGAGAAGAGACGATATTCAAGTAGTCTTTAATATGACAGGAACCATTTAATTGGAATATAAATTGTACTTTAACTTGAAGAACAAGAAATAAAATAAAATTTCCCAGATAATTGAAGAACTTTGATACCGCCCTTTGGAACAAGAAAGGGCTGGTTTTTTGCAGTTCTATGGGGCATCTAGATTCAAATGAATAATAGAATCTTTAGAAGCATGATAAGATCTTGAGAGGAGGTTTGTCCCTAGCTTGGTTTAACATCTTGGTCTAGGTGTTTGAGTCTAAACATGTTTGTCATTTATTAGGTTTGAGAGGAGTTTTGTCCCTAGCTTGGTTTAACATCTTGGTCTAGGTGTTTGAGTCTATACATTTTTGTCATTATTTATTAGGTTTTCTTTATTTTTTCTGAATGGAGCTCTTCCTTGTCTAGTTAGGTTAATTCCTTCTTGGCTCCTTTTGGTTTGCCCCGTTTGTGGGTTTTTGTTAGCTCATGTCGCAAGCCTCTATTTGGCCCTTTTTGTATTCTATCATTCTTTTTCGAAGAAAGTTTGGTTTTTTTTTTATTGTATTTGCACACATACAAACATATATATTTCAACATTTATCATGTGCAATTCAAGTAGTTGTAATCTTTATTTTCAGTTGCCTTTTCTCAATCATCTAGCCTCCTTTTTCCCCTTCTTCATTTCTTTCCAGTCATGCTTTTTGTGGCTGCCCCTGAGATGTGTCTTGGTGCAATATGTGTTTTTTTTTTAGTCTTCTATGTGAGTATTTGGGTAGCGTAGAGTTTAATTGTGCTACATTTAGACAAAATTATGACGTTTTCCTGTGTTCAGGCACAAGGAATGCGTAGGCATGTTGAAGAGATTGTTTTCTCTTTTACCTATCCTAGGCTTGATTTGGAGGTATGTGTATTACCTGACTTTATCTTAAGATTCTTAACTAGATTTTTTGCTCCAATTATTGGAATTAGGTCAATCTGTTCTAGCATTTAGCTTGGCATGCATACGGATTGCAACAAAAATTTCAAATTTACTTGTTAAAGCTTATTTTAATTTACTCCCTTGACGAGGCAAGTTCTTTATTGTTCAGGTTACTAAACACATGAACCATCTGCTAAAAGCACCATTCTGTGTTCATCCCAAAACAGGTCGGTGTTGGAATTAAATGCTAATGTTAGATCAGTTGTGACTTGCCGAAACCTCATTGTGGTCTCAATGTAAAAGATTCCTTTAGAGTCTTATAAAAAAATAATAATAAAATAATCATTAAAATGCACTTGATTTGGAATCCTGCAAGTAATTTGAGTAGAACGAAGGATGCAATTATTTCACTTTTGTGAAGAGAACATGTGTTTACATATTACTGAATCTCACTGTACCTTTTTATTCCAGGTCGTATTTGTGTCCCTATCGACCCTGAAAACTGTGATAAGTTTGATCCTACTACTGTGCCAACTGTTGCCCAGGTAAAACAACAAGAAGGCATATAG

mRNA sequence

ATGGTTACTGCAGCGGTTGAGAATGGCGGAAGCGAAATGGTAATTGACGGTAACGAACAGAAGCCGAAGAACATTGTGTCTGATGGGTTCGATGCCAATTATCTCAAAATTTACTACGGGAAGCTTTTTCCGTATGCTGATTTTTTCAAATGGATGTCTTATGGAAATGATGGCAAGCATCCGGGCAGTGACCAATCCTATTTTGGCCGAAGAGAGTTTTCATTCACCTTGGATAATGATATTTATCTGCGATTCCAATCTTTCAACAGTCTATCAGAAATGGAAAATTCAATCAAGGAAAAATGTCCATTTAAAATTGATATTGGGCCTGTATACAGCGTGGATCCTGCAAAGAGACATGCATATGCTGGCGATAATGTATTTACTCCAGTGGAGAGAGAGCTGGTTTTTGATATCGATATGACCGATTACGATGATGTTAGATATTGTTGTTCTGGAGCTGATGTTTGCTTGGATTGCTGGCCTTTAATGACAGCTGCTATCAAAGTGATTGATACTACCCTCCGAGATGACTTTGGATTTAACCACATTCTATGGGTATTTAGTGGTCGGCGTGGTGTTCATTGTTGGGTTTGTGATGGCAAAGCTAGAAGGTTGTCTAACGAACAGCGAGCTGCCATTGCTGAATATTTCCATTTATACCAGGGTAATGAAAATAGTCGCAAGAAAATTTCTCTAACGGGTCCAGTTCTTCATCCATTTCTTGCGAGATCGTACATAGAAGTTCTCAAGGATAGCTTTGAATCACAATTGCTCTATAGTCAAAAGATTTTCTCTACTGAGGAAAGATATGAGAAAATATTAGATATGATTCATGATGAATCCATTACCTCAGAGCTTCGAGGAAGGTGGCAAGATAATAGGCGACCCTCTAAAAATGATGTCAACTCAGTAAGATGGGAACAACTGAAAAATATGTTGCAATCAGGGAAACATAAGGCACAAGGAATGCGTAGGCATGTTGAAGAGATTGTTTTCTCTTTTACCTATCCTAGGCTTGATTTGGAGGTTACTAAACACATGAACCATCTGCTAAAAGCACCATTCTGTGTTCATCCCAAAACAGGTCGTATTTGTGTCCCTATCGACCCTGAAAACTGTGATAAGTTTGATCCTACTACTGTGCCAACTGTTGCCCAGGTAAAACAACAAGAAGGCATATAG

Coding sequence (CDS)

ATGGTTACTGCAGCGGTTGAGAATGGCGGAAGCGAAATGGTAATTGACGGTAACGAACAGAAGCCGAAGAACATTGTGTCTGATGGGTTCGATGCCAATTATCTCAAAATTTACTACGGGAAGCTTTTTCCGTATGCTGATTTTTTCAAATGGATGTCTTATGGAAATGATGGCAAGCATCCGGGCAGTGACCAATCCTATTTTGGCCGAAGAGAGTTTTCATTCACCTTGGATAATGATATTTATCTGCGATTCCAATCTTTCAACAGTCTATCAGAAATGGAAAATTCAATCAAGGAAAAATGTCCATTTAAAATTGATATTGGGCCTGTATACAGCGTGGATCCTGCAAAGAGACATGCATATGCTGGCGATAATGTATTTACTCCAGTGGAGAGAGAGCTGGTTTTTGATATCGATATGACCGATTACGATGATGTTAGATATTGTTGTTCTGGAGCTGATGTTTGCTTGGATTGCTGGCCTTTAATGACAGCTGCTATCAAAGTGATTGATACTACCCTCCGAGATGACTTTGGATTTAACCACATTCTATGGGTATTTAGTGGTCGGCGTGGTGTTCATTGTTGGGTTTGTGATGGCAAAGCTAGAAGGTTGTCTAACGAACAGCGAGCTGCCATTGCTGAATATTTCCATTTATACCAGGGTAATGAAAATAGTCGCAAGAAAATTTCTCTAACGGGTCCAGTTCTTCATCCATTTCTTGCGAGATCGTACATAGAAGTTCTCAAGGATAGCTTTGAATCACAATTGCTCTATAGTCAAAAGATTTTCTCTACTGAGGAAAGATATGAGAAAATATTAGATATGATTCATGATGAATCCATTACCTCAGAGCTTCGAGGAAGGTGGCAAGATAATAGGCGACCCTCTAAAAATGATGTCAACTCAGTAAGATGGGAACAACTGAAAAATATGTTGCAATCAGGGAAACATAAGGCACAAGGAATGCGTAGGCATGTTGAAGAGATTGTTTTCTCTTTTACCTATCCTAGGCTTGATTTGGAGGTTACTAAACACATGAACCATCTGCTAAAAGCACCATTCTGTGTTCATCCCAAAACAGGTCGTATTTGTGTCCCTATCGACCCTGAAAACTGTGATAAGTTTGATCCTACTACTGTGCCAACTGTTGCCCAGGTAAAACAACAAGAAGGCATATAG

Protein sequence

MVTAAVENGGSEMVIDGNEQKPKNIVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKHPGSDQSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRHAYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHPFLARSYIEVLKDSFESQLLYSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKNDVNSVRWEQLKNMLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPIDPENCDKFDPTTVPTVAQVKQQEGI
Homology
BLAST of HG10003554 vs. NCBI nr
Match: XP_038889665.1 (DNA primase small subunit [Benincasa hispida])

HSP 1 Score: 784.6 bits (2025), Expect = 3.9e-223
Identity = 372/391 (95.14%), Postives = 385/391 (98.47%), Query Frame = 0

Query: 1   MVTAAVENGGSEMVIDGNEQKPKNIVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60
           MVTAA+ENG S+MVIDGNEQKPKN VSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH
Sbjct: 1   MVTAAIENGESDMVIDGNEQKPKNTVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60

Query: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PG+DQSYFGRREFSFTLDND+YLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRH
Sbjct: 61  PGNDQSYFGRREFSFTLDNDVYLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180
           AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGA VCLDCWPLMTAAIKVIDTTLRDDFG
Sbjct: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGAGVCLDCWPLMTAAIKVIDTTLRDDFG 180

Query: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLARSYIEVLKDSFESQLLYSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN 300
           FLARSYIEVLKDSFESQLL SQKIFSTEERYEKILDMIHDESITSELRG+WQDNRR SKN
Sbjct: 241 FLARSYIEVLKDSFESQLLGSQKIFSTEERYEKILDMIHDESITSELRGKWQDNRRTSKN 300

Query: 301 DVNSVRWEQLKNMLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVNSVRWE+LKNMLQSGK+KAQGMRRHVEEIVFS+TYPRLDLEVTKHMNHLLKAPFCVHP
Sbjct: 301 DVNSVRWEKLKNMLQSGKYKAQGMRRHVEEIVFSYTYPRLDLEVTKHMNHLLKAPFCVHP 360

Query: 361 KTGRICVPIDPENCDKFDPTTVPTVAQVKQQ 392
           KTGRICVPIDPE+CD+FDPTTVPTVAQ+ ++
Sbjct: 361 KTGRICVPIDPEHCDEFDPTTVPTVAQLLEE 391

BLAST of HG10003554 vs. NCBI nr
Match: KAA0039338.1 (DNA primase, small subunit [Cucumis melo var. makuwa] >TYK00521.1 DNA primase, small subunit [Cucumis melo var. makuwa])

HSP 1 Score: 778.9 bits (2010), Expect = 2.1e-221
Identity = 371/391 (94.88%), Postives = 382/391 (97.70%), Query Frame = 0

Query: 1   MVTAAVENGGSEMVIDGNEQKPKNIVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60
           MVTAAVENGGS+MVIDGNEQKPK  VSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH
Sbjct: 16  MVTAAVENGGSDMVIDGNEQKPKKSVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 75

Query: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PGSDQSYFGRREFSFTLDNDIYLRFQSFN+LSEME SIKE+CPFKIDIGPVYSVDPAKRH
Sbjct: 76  PGSDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMEISIKEQCPFKIDIGPVYSVDPAKRH 135

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180
           AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG
Sbjct: 136 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 195

Query: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP
Sbjct: 196 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 255

Query: 241 FLARSYIEVLKDSFESQLLYSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN 300
           FLARSY EVLKDSFESQLL  QKIFSTEERYEKILDMI DESITS+LRGRWQDNRR SKN
Sbjct: 256 FLARSYTEVLKDSFESQLLCRQKIFSTEERYEKILDMIPDESITSDLRGRWQDNRRSSKN 315

Query: 301 DVNSVRWEQLKNMLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVNS+RWEQLKN+LQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP
Sbjct: 316 DVNSLRWEQLKNLLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 375

Query: 361 KTGRICVPIDPENCDKFDPTTVPTVAQVKQQ 392
           KTGRICVPIDPE+CD+FDPTTVPTVAQ+ ++
Sbjct: 376 KTGRICVPIDPEHCDEFDPTTVPTVAQLLEE 406

BLAST of HG10003554 vs. NCBI nr
Match: KAG6592333.1 (DNA primase small subunit, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 774.6 bits (1999), Expect = 4.0e-220
Identity = 367/391 (93.86%), Postives = 380/391 (97.19%), Query Frame = 0

Query: 1   MVTAAVENGGSEMVIDGNEQKPKNIVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60
           MV  A ENGGS+MVIDGNEQKPKNIVS+GFDANYLKIYYGKLFP+ADFFKWMSYGNDGKH
Sbjct: 1   MVHEAAENGGSDMVIDGNEQKPKNIVSNGFDANYLKIYYGKLFPFADFFKWMSYGNDGKH 60

Query: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PGSD SY GRREFSFTLDNDIYLRFQSFNSLSEME+S KEKCPFKIDIGPVYSVDPAKRH
Sbjct: 61  PGSDHSYIGRREFSFTLDNDIYLRFQSFNSLSEMESSTKEKCPFKIDIGPVYSVDPAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180
           AYAG NVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVID+TLRDDFG
Sbjct: 121 AYAGANVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDSTLRDDFG 180

Query: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIA+YFHLYQGNENSRKKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIADYFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLARSYIEVLKDSFESQLLYSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN 300
           FLARSYIEVLKDSFES+LL SQKIFSTEERYEK+LDMIHDESITSELRGRWQDNRRPSKN
Sbjct: 241 FLARSYIEVLKDSFESKLLCSQKIFSTEERYEKVLDMIHDESITSELRGRWQDNRRPSKN 300

Query: 301 DVNSVRWEQLKNMLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVN VRWEQLKNMLQSGKHK+QGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP
Sbjct: 301 DVNLVRWEQLKNMLQSGKHKSQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360

Query: 361 KTGRICVPIDPENCDKFDPTTVPTVAQVKQQ 392
           KTGRICVPIDPE CD+FDPTTVPTVAQ+ ++
Sbjct: 361 KTGRICVPIDPERCDEFDPTTVPTVAQLLEE 391

BLAST of HG10003554 vs. NCBI nr
Match: XP_004141619.1 (DNA primase small subunit [Cucumis sativus] >XP_031742330.1 DNA primase small subunit [Cucumis sativus] >KGN52619.1 hypothetical protein Csa_007851 [Cucumis sativus])

HSP 1 Score: 773.9 bits (1997), Expect = 6.8e-220
Identity = 369/391 (94.37%), Postives = 380/391 (97.19%), Query Frame = 0

Query: 1   MVTAAVENGGSEMVIDGNEQKPKNIVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60
           MVTAAVENGGS+MVIDGNEQKPKN VSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH
Sbjct: 1   MVTAAVENGGSDMVIDGNEQKPKNTVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60

Query: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PGSDQSYFGRREFSFTLDNDIYLRFQSFN+LSEME SIKEKCPFKIDIGPVYSVDPAKRH
Sbjct: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMEISIKEKCPFKIDIGPVYSVDPAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180
           AYA DNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVI TTLRDDFG
Sbjct: 121 AYADDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIHTTLRDDFG 180

Query: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLARSYIEVLKDSFESQLLYSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN 300
           FL RSY EVLKDSFESQLL SQKIFSTEERYEKILDMI DESITS+LRGRWQDNRR SKN
Sbjct: 241 FLVRSYTEVLKDSFESQLLCSQKIFSTEERYEKILDMIPDESITSDLRGRWQDNRRSSKN 300

Query: 301 DVNSVRWEQLKNMLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVNS+RWEQLK++LQSGKHKAQGMRRHVEEIVFSFTYPRLD EVTKHMNHLLKAPFCVHP
Sbjct: 301 DVNSLRWEQLKSLLQSGKHKAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHP 360

Query: 361 KTGRICVPIDPENCDKFDPTTVPTVAQVKQQ 392
           KTGRICVPIDPE+CD+FDPTTVPTVAQ+ ++
Sbjct: 361 KTGRICVPIDPEHCDEFDPTTVPTVAQLLEE 391

BLAST of HG10003554 vs. NCBI nr
Match: XP_023535939.1 (DNA primase small subunit [Cucurbita pepo subsp. pepo])

HSP 1 Score: 773.1 bits (1995), Expect = 1.2e-219
Identity = 368/391 (94.12%), Postives = 379/391 (96.93%), Query Frame = 0

Query: 1   MVTAAVENGGSEMVIDGNEQKPKNIVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60
           MV  A ENGGS+MVIDGNEQKPKNIVS+ FDANYLKIYYGKLFP+ADFFKWMSYGNDGKH
Sbjct: 1   MVHEAAENGGSDMVIDGNEQKPKNIVSNVFDANYLKIYYGKLFPFADFFKWMSYGNDGKH 60

Query: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PGSD SY GRREFSFTLDNDIYLRFQSFNSLSEME+S KEKCPFKIDIGPVYSVDPAKRH
Sbjct: 61  PGSDHSYIGRREFSFTLDNDIYLRFQSFNSLSEMESSTKEKCPFKIDIGPVYSVDPAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180
           AYAG NVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG
Sbjct: 121 AYAGANVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180

Query: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIA+YFHLYQGNENSRKKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIADYFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLARSYIEVLKDSFESQLLYSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN 300
           FLARSYIEVLKDSFES+LL SQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN
Sbjct: 241 FLARSYIEVLKDSFESKLLCSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN 300

Query: 301 DVNSVRWEQLKNMLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVN VRWEQLKNMLQSGKHK+QGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP
Sbjct: 301 DVNLVRWEQLKNMLQSGKHKSQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360

Query: 361 KTGRICVPIDPENCDKFDPTTVPTVAQVKQQ 392
           KTGRICVPIDPE CD+FDPTTVPTVAQ+ ++
Sbjct: 361 KTGRICVPIDPERCDEFDPTTVPTVAQLLEE 391

BLAST of HG10003554 vs. ExPASy Swiss-Prot
Match: P20664 (DNA primase small subunit OS=Mus musculus OX=10090 GN=Prim1 PE=1 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 6.9e-90
Identity = 171/356 (48.03%), Postives = 236/356 (66.29%), Query Frame = 0

Query: 35  LKIYYGKLFPYADFFKWMSYGNDGKHPGSDQSYFGRREFSFTLDNDIYLRFQSFNSLSEM 94
           LK+YY +LFPYA +++W++YG      G  ++YF  REFSFTL +DIY+R+QSFN+ SE+
Sbjct: 13  LKLYYRRLFPYAQYYRWLNYG------GVTKNYFQHREFSFTLKDDIYIRYQSFNNQSEL 72

Query: 95  ENSIKEKCPFKIDIGPVYSVDPAKRHAYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGA 154
           E  +++  P+KIDIG VYS  P  +H       F   E+ELVFDIDMTDYDDVR CCS A
Sbjct: 73  EKEMQKMNPYKIDIGAVYSHRP-NQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSA 132

Query: 155 DVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI 214
           D+C  CW LMT A+++ID  L++DFGF H LWV+SGRRGVHCWVCD   R+LS+  R+ I
Sbjct: 133 DICSKCWTLMTMAMRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGI 192

Query: 215 AEYFHLYQGNENSRKKISLTGPVLHPFLARSYIEVLKDSFESQLLYSQKIFSTEERYEKI 274
            EY  L +G ++ +KK+ L   V HPF+ +S I ++K  FE   L  Q I   +E ++KI
Sbjct: 193 VEYLSLVKGGQDVKKKVHLNEKV-HPFVRKS-INIIKKYFEEYALVGQDILENKENWDKI 252

Query: 275 LDMIHDESITSELRGRWQDNRRPSKNDVNSVRWEQLKNMLQSGKH-KAQGMRRHVE-EIV 334
           L ++  E+I  EL+  +Q      K   +  RWE L+ +  S ++ K       +E E++
Sbjct: 253 LALV-PETIHDELQRGFQ------KFHSSPQRWEHLRKVANSSQNMKNDKCGPWLEWEVM 312

Query: 335 FSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPIDPENCDKFDPTTVPTVAQV 389
             + +PRLD+ V+K +NHLLK+PF VHPKTGRI VPID    D+FDP TVPT++ +
Sbjct: 313 LQYCFPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDFHKVDQFDPFTVPTISAI 352

BLAST of HG10003554 vs. ExPASy Swiss-Prot
Match: P49642 (DNA primase small subunit OS=Homo sapiens OX=9606 GN=PRIM1 PE=1 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 2.0e-89
Identity = 168/355 (47.32%), Postives = 237/355 (66.76%), Query Frame = 0

Query: 35  LKIYYGKLFPYADFFKWMSYGNDGKHPGSDQSYFGRREFSFTLDNDIYLRFQSFNSLSEM 94
           LK+YY +LFPY+ +++W++YG      G  ++YF  REFSFTL +DIY+R+QSFN+ S++
Sbjct: 13  LKLYYRRLFPYSQYYRWLNYG------GVIKNYFQHREFSFTLKDDIYIRYQSFNNQSDL 72

Query: 95  ENSIKEKCPFKIDIGPVYSVDPAKRHAYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGA 154
           E  +++  P+KIDIG VYS  P  +H       F   E+ELVFDIDMTDYDDVR CCS A
Sbjct: 73  EKEMQKMNPYKIDIGAVYSHRP-NQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSA 132

Query: 155 DVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI 214
           D+C  CW LMT AI++ID  L++DFGF H LWV+SGRRGVHCWVCD   R+LS+  R+ I
Sbjct: 133 DICPKCWTLMTMAIRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGI 192

Query: 215 AEYFHLYQGNENSRKKISLTGPVLHPFLARSYIEVLKDSFESQLLYSQKIFSTEERYEKI 274
            EY  L +G ++ +KK+ L+  + HPF+ +S I ++K  FE   L +Q I   +E ++KI
Sbjct: 193 VEYLSLVKGGQDVKKKVHLSEKI-HPFIRKS-INIIKKYFEEYALVNQDILENKESWDKI 252

Query: 275 LDMIHDESITSELRGRWQDNRRPSKNDVNSVRWEQLKNMLQSGKHKAQGMRRH---VEEI 334
           L ++  E+I  EL+  +Q      K+  +  RWE LK +    ++  +  +       EI
Sbjct: 253 LALV-PETIHDELQQSFQ------KSHNSLQRWEHLKKVASRYQNNIKNDKYGPWLEWEI 312

Query: 335 VFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPIDPENCDKFDPTTVPTVA 387
           +  + +PRLD+ V+K +NHLLK+PF VHPKTGRI VPID +  D+FDP TVPT++
Sbjct: 313 MLQYCFPRLDINVSKGINHLLKSPFSVHPKTGRISVPIDLQKVDQFDPFTVPTIS 351

BLAST of HG10003554 vs. ExPASy Swiss-Prot
Match: Q24317 (DNA primase small subunit OS=Drosophila melanogaster OX=7227 GN=Prim1 PE=1 SV=2)

HSP 1 Score: 312.0 bits (798), Expect = 9.6e-84
Identity = 158/361 (43.77%), Postives = 219/361 (60.66%), Query Frame = 0

Query: 35  LKIYYGKLFPYADFFKWMSYGNDGKHPGSDQSYFGRREFSFTLDNDIYLRFQSFNSLSEM 94
           L +YY +LFP+  F++W+SYG+      S+ + F  RE SFTL +DIY+R+  F++ +E+
Sbjct: 32  LPVYYRRLFPHLPFYRWLSYGS------SEDAIFSNREISFTLQDDIYIRYLCFDTQAEL 91

Query: 95  ENSIKEKCPFKIDIGPVYSVDPAKRHAYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGA 154
           E  I  + P KIDIGPV    P    +  G    TPV+RELVFDIDMTDYD+VR CCSGA
Sbjct: 92  EKEICSRNPIKIDIGPVMHSKPKNHRSIPGG--LTPVQRELVFDIDMTDYDEVRTCCSGA 151

Query: 155 DVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAI 214
            VCL CW  M  A +V+D  LR+DFGF HI+W+FSGRRG+HCWVCD +AR L    R A+
Sbjct: 152 GVCLKCWKFMVLAARVLDVALREDFGFEHIIWIFSGRRGIHCWVCDYQARHLDGRGRYAV 211

Query: 215 AEYFHL--YQGNENSRKKISLTGPVLHPFLARSYIEVLKDSFESQLLYSQKIFSTEERYE 274
           AEY ++  Y             G   H  L R+ +++++  FE  +L  Q +F T +   
Sbjct: 212 AEYLNIITYASFAGGNSPRCSMGDRPHHSLKRA-LKIVEPMFEEIVLEDQNLFGTPKGVT 271

Query: 275 KILDMIHDESITSELRGRWQDNRRPSKNDVNSVRWE---QLKNMLQSGKHKA--QGMRRH 334
           K+L+M+HD++   EL    Q N     +  + + WE   +  N +++    A  + ++  
Sbjct: 272 KLLNMVHDDAARGELESYMQKNLEDGAH--SRLVWESFIKYANSMRTSTTSAWSRKLKNI 331

Query: 335 VEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPIDPENCDKFDPTTVPTVAQ 389
           V EI     YPRLD+ VT+  NHLLKAPFC+HP TG++CVP       KFDPTTVPT+ Q
Sbjct: 332 VAEIQLGLLYPRLDINVTRGFNHLLKAPFCIHPATGKVCVPFSVSAVAKFDPTTVPTITQ 381

BLAST of HG10003554 vs. ExPASy Swiss-Prot
Match: O14215 (DNA primase small subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pri1 PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 5.0e-80
Identity = 155/371 (41.78%), Postives = 225/371 (60.65%), Query Frame = 0

Query: 31  DANYLKIYYGKLFPYADFFKWMSYG----NDGKHPGSDQSYFGRREFSFTLDNDIYLRFQ 90
           D+  +  YY  LFP+   F+W+++G    ND          F  REF+FTL ND Y+R+ 
Sbjct: 31  DSETMIQYYRHLFPWKYLFQWLNHGPVVTND----------FAHREFAFTLPNDAYIRYL 90

Query: 91  SFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRHAYAGDNVFTPVERELVFDIDMTDYDD 150
           SF++  E++      CP + ++GPVYS +P  R      + F P+++ELVFDIDMTDYDD
Sbjct: 91  SFSNWEELKKEALNLCPSRFEVGPVYSANPRDRKTIR-KSTFHPLKKELVFDIDMTDYDD 150

Query: 151 VRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSGRRGVHCWVCDGKARRL 210
           VR CCS  ++C  CWP +T A++V+D    +DFGF HILWV+SGRRG+H W+CD  A  L
Sbjct: 151 VRTCCSKTNICEKCWPFITIAVQVLDICFHEDFGFKHILWVYSGRRGIHAWICDEIACSL 210

Query: 211 SNEQRAAIAEYFHLYQGNENSRKKI--SLTGPVLHPFLARSYIEVLKDSFESQLLYSQKI 270
            +  R  IA Y  +  GN     ++  +L  P LHP L RS + +LK +F   +L  Q  
Sbjct: 211 DDRSRRMIASYLQVVVGNPQGGVRLINNLKRP-LHPHLTRS-LNILKSAFVKIVLEDQDP 270

Query: 271 FSTEERYEKILDMIHDESITSELRGRWQ-DNRRPSKNDVNSVRWEQLKNMLQSGKHKA-- 330
           ++++E  E +L ++ D+ + S LR +W+ D  R SKN     +W  +  +L SG   +  
Sbjct: 271 WASKEGAENLLKLLPDKDLASALRKKWEVDPERSSKN-----KWSDIDTVLASGSIASIS 330

Query: 331 -QGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPIDPENCDKFDPT 390
              +    ++IV ++ YPRLD+EV++H+NHLLK+PFCVHP T R+CVPID E  D F+P 
Sbjct: 331 PSVIAIAKQDIVLTYLYPRLDVEVSRHLNHLLKSPFCVHPGTSRVCVPIDIERMDSFNPL 383

Query: 391 TVPTVAQVKQQ 392
            VPTV  + Q+
Sbjct: 391 KVPTVNDLLQE 383

BLAST of HG10003554 vs. ExPASy Swiss-Prot
Match: Q7KQM1 (DNA primase small subunit OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=PF14_0366 PE=3 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 8.5e-72
Identity = 148/385 (38.44%), Postives = 225/385 (58.44%), Query Frame = 0

Query: 33  NYLKIYYGKLFPYADFFKWMSYGND--GKHPG-SDQSYFGRREFSFTL------DNDIYL 92
           N L  YY  L P  D + W++Y ND  GK+   +D  +F +REFSFT         +IY+
Sbjct: 17  NDLIFYYRSLCPINDLYNWLNYKNDIKGKYTKLNDPHFFSKREFSFTCKKSDQGKEEIYI 76

Query: 93  RFQSFNSLSEMENS-IKEKCPFKIDIGPVYSVDPAKRHAYAGDNVFTPVERELVFDIDMT 152
           R+ SF++  E +N  + +  P K DIG +Y+  P  +    GD +F PV++EL+FDIDM 
Sbjct: 77  RWLSFSNPEEFKNKLLSDLVPIKFDIGAIYNF-PVSQKDQKGD-IFLPVQKELIFDIDMN 136

Query: 153 DYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSGRRGVHCWVCDGK 212
           DYDD+R CC+   VC  CW  +T AI ++DT LR+DF F HILWV+SGRRG+HCWV D  
Sbjct: 137 DYDDIRTCCTDKKVCKLCWKFLTVAIVLLDTALREDFSFEHILWVYSGRRGIHCWVADES 196

Query: 213 ARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHPFLARSYIEVLKDSFESQLLYSQ 272
            R  + + RAA+A+Y ++  G++  +KK+S+ G   +P   R++    K  +   L+  Q
Sbjct: 197 CRYYTTDARAALADYLNILSGSDTKKKKVSIWGKDKYPMFERAFDICYK--YFDVLMEEQ 256

Query: 273 KIFST-EERYEKILDMIHDES--ITSELRGRWQDNRRP--SKNDVNS----VRWEQLKNM 332
             F       +K++D +   S  +T  L+    +  +   + N+ NS     ++  + N 
Sbjct: 257 DFFKKGSPHVQKLIDYLPYASGKVTDPLKAMKLNELKEYINNNNFNSREIFEKFSSIYNF 316

Query: 333 LQSGKHKAQ-------GMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRIC 392
           L    +  +        M   V+EIVF FTYPRLD+ V+K +NHLLK+PFC+H  TGR+C
Sbjct: 317 LTPSNYFKRKNVSGNINMPSFVKEIVFHFTYPRLDINVSKEINHLLKSPFCIHNSTGRVC 376

BLAST of HG10003554 vs. ExPASy TrEMBL
Match: A0A5A7T8Y3 (DNA primase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001310 PE=3 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 1.0e-221
Identity = 371/391 (94.88%), Postives = 382/391 (97.70%), Query Frame = 0

Query: 1   MVTAAVENGGSEMVIDGNEQKPKNIVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60
           MVTAAVENGGS+MVIDGNEQKPK  VSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH
Sbjct: 16  MVTAAVENGGSDMVIDGNEQKPKKSVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 75

Query: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PGSDQSYFGRREFSFTLDNDIYLRFQSFN+LSEME SIKE+CPFKIDIGPVYSVDPAKRH
Sbjct: 76  PGSDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMEISIKEQCPFKIDIGPVYSVDPAKRH 135

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180
           AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG
Sbjct: 136 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 195

Query: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP
Sbjct: 196 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 255

Query: 241 FLARSYIEVLKDSFESQLLYSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN 300
           FLARSY EVLKDSFESQLL  QKIFSTEERYEKILDMI DESITS+LRGRWQDNRR SKN
Sbjct: 256 FLARSYTEVLKDSFESQLLCRQKIFSTEERYEKILDMIPDESITSDLRGRWQDNRRSSKN 315

Query: 301 DVNSVRWEQLKNMLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVNS+RWEQLKN+LQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP
Sbjct: 316 DVNSLRWEQLKNLLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 375

Query: 361 KTGRICVPIDPENCDKFDPTTVPTVAQVKQQ 392
           KTGRICVPIDPE+CD+FDPTTVPTVAQ+ ++
Sbjct: 376 KTGRICVPIDPEHCDEFDPTTVPTVAQLLEE 406

BLAST of HG10003554 vs. ExPASy TrEMBL
Match: A0A0A0KUP7 (DNA primase OS=Cucumis sativus OX=3659 GN=Csa_5G647410 PE=3 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 3.3e-220
Identity = 369/391 (94.37%), Postives = 380/391 (97.19%), Query Frame = 0

Query: 1   MVTAAVENGGSEMVIDGNEQKPKNIVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60
           MVTAAVENGGS+MVIDGNEQKPKN VSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH
Sbjct: 1   MVTAAVENGGSDMVIDGNEQKPKNTVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60

Query: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PGSDQSYFGRREFSFTLDNDIYLRFQSFN+LSEME SIKEKCPFKIDIGPVYSVDPAKRH
Sbjct: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNNLSEMEISIKEKCPFKIDIGPVYSVDPAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180
           AYA DNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVI TTLRDDFG
Sbjct: 121 AYADDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIHTTLRDDFG 180

Query: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLARSYIEVLKDSFESQLLYSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN 300
           FL RSY EVLKDSFESQLL SQKIFSTEERYEKILDMI DESITS+LRGRWQDNRR SKN
Sbjct: 241 FLVRSYTEVLKDSFESQLLCSQKIFSTEERYEKILDMIPDESITSDLRGRWQDNRRSSKN 300

Query: 301 DVNSVRWEQLKNMLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVNS+RWEQLK++LQSGKHKAQGMRRHVEEIVFSFTYPRLD EVTKHMNHLLKAPFCVHP
Sbjct: 301 DVNSLRWEQLKSLLQSGKHKAQGMRRHVEEIVFSFTYPRLDSEVTKHMNHLLKAPFCVHP 360

Query: 361 KTGRICVPIDPENCDKFDPTTVPTVAQVKQQ 392
           KTGRICVPIDPE+CD+FDPTTVPTVAQ+ ++
Sbjct: 361 KTGRICVPIDPEHCDEFDPTTVPTVAQLLEE 391

BLAST of HG10003554 vs. ExPASy TrEMBL
Match: A0A6J1EEZ9 (DNA primase OS=Cucurbita moschata OX=3662 GN=LOC111432676 PE=3 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 7.4e-220
Identity = 367/391 (93.86%), Postives = 379/391 (96.93%), Query Frame = 0

Query: 1   MVTAAVENGGSEMVIDGNEQKPKNIVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60
           MV  A ENGGS+MVIDGNEQKPKNIVS+GFDANYL+IYYGKLFP+ADFFKWMSYGNDGKH
Sbjct: 1   MVHEAAENGGSDMVIDGNEQKPKNIVSNGFDANYLEIYYGKLFPFADFFKWMSYGNDGKH 60

Query: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PGSD SY GRREFSFTLDNDIYLRFQSFNSLSEME+S KEKCPFKIDIGPVYSVDPAKRH
Sbjct: 61  PGSDHSYIGRREFSFTLDNDIYLRFQSFNSLSEMESSTKEKCPFKIDIGPVYSVDPAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180
           AYAG NVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG
Sbjct: 121 AYAGANVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180

Query: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIA+YFHLYQGNENSRKKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIADYFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLARSYIEVLKDSFESQLLYSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN 300
           FLARSYIEVLKDSFES+LL SQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN
Sbjct: 241 FLARSYIEVLKDSFESKLLCSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN 300

Query: 301 DVNSVRWEQLKNMLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVN VRWEQLKNMLQSGKHK+ GMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP
Sbjct: 301 DVNLVRWEQLKNMLQSGKHKSPGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360

Query: 361 KTGRICVPIDPENCDKFDPTTVPTVAQVKQQ 392
           KTGRICVPIDPE CD+FDPTTVPTVAQ+ ++
Sbjct: 361 KTGRICVPIDPERCDEFDPTTVPTVAQLLEE 391

BLAST of HG10003554 vs. ExPASy TrEMBL
Match: A0A6J1I8T4 (DNA primase OS=Cucurbita maxima OX=3661 GN=LOC111472037 PE=3 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 7.6e-217
Identity = 361/381 (94.75%), Postives = 373/381 (97.90%), Query Frame = 0

Query: 11  SEMVIDGNEQKPKNIVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKHPGSDQSYFGR 70
           ++MVIDGNEQKPKNIVS+GFDANYLKIYYGKLFP+ADFFKWMSYGNDGKHPGSD SY GR
Sbjct: 6   ADMVIDGNEQKPKNIVSNGFDANYLKIYYGKLFPFADFFKWMSYGNDGKHPGSDHSYVGR 65

Query: 71  REFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRHAYAGDNVFTP 130
           REFSFTLDNDIYLRFQSFNSLSEME+S KEKCPFKIDIGPVYSVDPAKRHAYAG NVFTP
Sbjct: 66  REFSFTLDNDIYLRFQSFNSLSEMESSTKEKCPFKIDIGPVYSVDPAKRHAYAGANVFTP 125

Query: 131 VERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSG 190
           VERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSG
Sbjct: 126 VERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFGFNHILWVFSG 185

Query: 191 RRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHPFLARSYIEVL 250
           RRGVHCWVCDGKARRLSNEQRAAIA+YFHLYQGNENSRKKISLTGPVLHPFLARSYIEVL
Sbjct: 186 RRGVHCWVCDGKARRLSNEQRAAIADYFHLYQGNENSRKKISLTGPVLHPFLARSYIEVL 245

Query: 251 KDSFESQLLYSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKNDVNSVRWEQL 310
           KDSFES+LL SQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKNDVN VRWEQL
Sbjct: 246 KDSFESKLLCSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKNDVNLVRWEQL 305

Query: 311 KNMLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPID 370
           KNMLQSGKHK+QGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPID
Sbjct: 306 KNMLQSGKHKSQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHPKTGRICVPID 365

Query: 371 PENCDKFDPTTVPTVAQVKQQ 392
           PE CD+FDPTTVPTVAQ+ ++
Sbjct: 366 PERCDEFDPTTVPTVAQLLEE 386

BLAST of HG10003554 vs. ExPASy TrEMBL
Match: A0A6J1CKZ3 (DNA primase OS=Momordica charantia OX=3673 GN=LOC111012104 PE=3 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 5.1e-213
Identity = 354/391 (90.54%), Postives = 373/391 (95.40%), Query Frame = 0

Query: 1   MVTAAVENGGSEMVIDGNEQKPKNIVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60
           M+T AVENGG +M IDG EQKP+ +V DGFD NYLKIYYGKLFPY DFFKWMSYGNDGKH
Sbjct: 1   MITEAVENGGGDMEIDGFEQKPEKVVPDGFDVNYLKIYYGKLFPYTDFFKWMSYGNDGKH 60

Query: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PGSDQSYFGRREFSFTL+NDIYLR+QSFN+LSEMENSIKEKCPFKIDIGPVYSVD AKRH
Sbjct: 61  PGSDQSYFGRREFSFTLENDIYLRYQSFNNLSEMENSIKEKCPFKIDIGPVYSVDLAKRH 120

Query: 121 AYAGDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180
           AYAGDNVFTPVEREL+FDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG
Sbjct: 121 AYAGDNVFTPVERELIFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDDFG 180

Query: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVLHP 240
           FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAE+FHLYQGNENSRKKISLTGPVLHP
Sbjct: 181 FNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEHFHLYQGNENSRKKISLTGPVLHP 240

Query: 241 FLARSYIEVLKDSFESQLLYSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPSKN 300
           FLARSY EVLKDSFES LL SQKIFSTEERYEKILDMI DES+TSELRGRWQ+NR+PSK 
Sbjct: 241 FLARSYTEVLKDSFESTLLCSQKIFSTEERYEKILDMIPDESVTSELRGRWQENRQPSKK 300

Query: 301 DVNSVRWEQLKNMLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPFCVHP 360
           DVN VRWEQLK+MLQS KHKAQGMRRHVEEIVFSFTYPRLDLEVTKH NHLLKAPFCVHP
Sbjct: 301 DVNYVRWEQLKSMLQSVKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHTNHLLKAPFCVHP 360

Query: 361 KTGRICVPIDPENCDKFDPTTVPTVAQVKQQ 392
           KTGRICVPI+PENCD+FDPTTVPT+AQ+ ++
Sbjct: 361 KTGRICVPINPENCDEFDPTTVPTLAQLLEE 391

BLAST of HG10003554 vs. TAIR 10
Match: AT5G41880.1 (DNA primases;DNA primases )

HSP 1 Score: 555.4 bits (1430), Expect = 3.6e-158
Identity = 260/395 (65.82%), Postives = 320/395 (81.01%), Query Frame = 0

Query: 1   MVTAAVENGGSEMVIDGNEQKPKNIVSDGFDANYLKIYYGKLFPYADFFKWMSYGNDGKH 60
           M     E  G +M+ID  + KPK  + + F+ +YL+IYYG LFPY D  KW+SYG+DGKH
Sbjct: 1   MTREETETSGEDMMID--DPKPK--IENDFNVHYLRIYYGNLFPYTDIHKWLSYGHDGKH 60

Query: 61  PGSDQSYFGRREFSFTLDNDIYLRFQSFNSLSEMENSIKEKCPFKIDIGPVYSVDPAKRH 120
           PG D+ YFGRREFSFTL+ND+YLR++SF + S ME +IK   P+KIDIG VYSVDP KRH
Sbjct: 61  PGCDEYYFGRREFSFTLENDVYLRYKSFKNASLMEAAIKSNFPYKIDIGAVYSVDPDKRH 120

Query: 121 AYA--GDNVFTPVERELVFDIDMTDYDDVRYCCSGADVCLDCWPLMTAAIKVIDTTLRDD 180
           AYA  G N+FTPVERELVFDID+TDYDDVRYCCSGADVC  CWPLMT AIKVIDT+LR+D
Sbjct: 121 AYAQSGTNLFTPVERELVFDIDITDYDDVRYCCSGADVCSKCWPLMTVAIKVIDTSLRED 180

Query: 181 FGFNHILWVFSGRRGVHCWVCDGKARRLSNEQRAAIAEYFHLYQGNENSRKKISLTGPVL 240
           FGF HILWVFSGRRGVHCWVCD KARRL+NEQR+A+AEYF +Y+GNEN+ KK+ L G  L
Sbjct: 181 FGFKHILWVFSGRRGVHCWVCDAKARRLTNEQRSAVAEYFRVYKGNENNAKKVDLMGHSL 240

Query: 241 HPFLARSYIEVLKDSFESQLLYSQKIFSTEERYEKILDMIHDESITSELRGRWQDNRRPS 300
           HPFLARSY++ +K  FE +L  +Q IFS++E+YEKIL MI DE I ++LRG+W+++ R S
Sbjct: 241 HPFLARSYVDFIKSFFEGELQATQSIFSSKEKYEKILGMITDEDIQADLRGKWENSARSS 300

Query: 301 KND--VNSVRWEQLKNMLQSGKHKAQGMRRHVEEIVFSFTYPRLDLEVTKHMNHLLKAPF 360
            ++   + +RWEQLK  LQS ++KA  +R  VEEIVF+FTYPR+DLEV+K MNHLLKAPF
Sbjct: 301 LSEEATSLLRWEQLKKALQSKRNKALSLRTCVEEIVFTFTYPRIDLEVSKQMNHLLKAPF 360

Query: 361 CVHPKTGRICVPIDPENCDKFDPTTVPTVAQVKQQ 392
           CVHPKTGR+CVPIDP NCD+FDP  VPT++Q+ ++
Sbjct: 361 CVHPKTGRVCVPIDPNNCDEFDPLAVPTLSQLIEE 391

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038889665.13.9e-22395.14DNA primase small subunit [Benincasa hispida][more]
KAA0039338.12.1e-22194.88DNA primase, small subunit [Cucumis melo var. makuwa] >TYK00521.1 DNA primase, s... [more]
KAG6592333.14.0e-22093.86DNA primase small subunit, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_004141619.16.8e-22094.37DNA primase small subunit [Cucumis sativus] >XP_031742330.1 DNA primase small su... [more]
XP_023535939.11.2e-21994.12DNA primase small subunit [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P206646.9e-9048.03DNA primase small subunit OS=Mus musculus OX=10090 GN=Prim1 PE=1 SV=1[more]
P496422.0e-8947.32DNA primase small subunit OS=Homo sapiens OX=9606 GN=PRIM1 PE=1 SV=1[more]
Q243179.6e-8443.77DNA primase small subunit OS=Drosophila melanogaster OX=7227 GN=Prim1 PE=1 SV=2[more]
O142155.0e-8041.78DNA primase small subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843)... [more]
Q7KQM18.5e-7238.44DNA primase small subunit OS=Plasmodium falciparum (isolate 3D7) OX=36329 GN=PF1... [more]
Match NameE-valueIdentityDescription
A0A5A7T8Y31.0e-22194.88DNA primase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001310 P... [more]
A0A0A0KUP73.3e-22094.37DNA primase OS=Cucumis sativus OX=3659 GN=Csa_5G647410 PE=3 SV=1[more]
A0A6J1EEZ97.4e-22093.86DNA primase OS=Cucurbita moschata OX=3662 GN=LOC111432676 PE=3 SV=1[more]
A0A6J1I8T47.6e-21794.75DNA primase OS=Cucurbita maxima OX=3661 GN=LOC111472037 PE=3 SV=1[more]
A0A6J1CKZ35.1e-21390.54DNA primase OS=Momordica charantia OX=3673 GN=LOC111012104 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G41880.13.6e-15865.82DNA primases;DNA primases [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002755DNA primase, small subunitPFAMPF01896DNA_primase_Scoord: 137..370
e-value: 2.3E-61
score: 207.0
NoneNo IPR availableGENE3D3.90.920.10DNA primase, PRIM domaincoord: 23..392
e-value: 6.4E-146
score: 488.4
NoneNo IPR availablePANTHERPTHR10536:SF1DNA PRIMASEcoord: 41..389
NoneNo IPR availableSUPERFAMILY56747Prim-pol domaincoord: 32..389
IPR014052DNA primase, small subunit, eukaryotic/archaealTIGRFAMTIGR00335TIGR00335coord: 36..385
e-value: 1.5E-66
score: 223.0
IPR014052DNA primase, small subunit, eukaryotic/archaealPANTHERPTHR10536DNA PRIMASE SMALL SUBUNITcoord: 41..389
IPR014052DNA primase, small subunit, eukaryotic/archaealCDDcd04860AE_Prim_Scoord: 44..379
e-value: 3.36605E-97
score: 287.588

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10003554.1HG10003554.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006269 DNA replication, synthesis of RNA primer
cellular_component GO:0005658 alpha DNA polymerase:primase complex
molecular_function GO:0003896 DNA primase activity
molecular_function GO:0046872 metal ion binding