HG10003420 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10003420
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	E3 ubiquitin-protein ligase
Location	Chr08: 1065077 .. 1065364 (+)
RNA-Seq Expression	HG10003420
Synteny	HG10003420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCACAAGCAACGAGTGCTCCCATTTCTTCTGCAAAGATTGCATCTCTAATCACATAGCGGCCAAAGTGGAAGACAATATAGCCAACATCAAGTGTCCAGAGCCAGGTTGTGAGACAGTGCTAGACCCCAACATCTGCCATTCATTCCTCCCCAAAAACCTGTTGGATCGCTGGGGAGATGTGTTATGTGAGGTCGCCATTCTCGGCATCCAGAGACTCTACTGCCCCTTCGTGGACTGCTCGGTGATGCTTATCAATGATGGAGGCCGAGTGTCTGAGCTGTAA

mRNA sequence

ATGTTCACAAGCAACGAGTGCTCCCATTTCTTCTGCAAAGATTGCATCTCTAATCACATAGCGGCCAAAGTGGAAGACAATATAGCCAACATCAAGTGTCCAGAGCCAGGTTGTGAGACAGTGCTAGACCCCAACATCTGCCATTCATTCCTCCCCAAAAACCTGTTGGATCGCTGGGGAGATGTGTTATGTGAGGTCGCCATTCTCGGCATCCAGAGACTCTACTGCCCCTTCGTGGACTGCTCGGTGATGCTTATCAATGATGGAGGCCGAGTGTCTGAGCTGTAA

Coding sequence (CDS)

ATGTTCACAAGCAACGAGTGCTCCCATTTCTTCTGCAAAGATTGCATCTCTAATCACATAGCGGCCAAAGTGGAAGACAATATAGCCAACATCAAGTGTCCAGAGCCAGGTTGTGAGACAGTGCTAGACCCCAACATCTGCCATTCATTCCTCCCCAAAAACCTGTTGGATCGCTGGGGAGATGTGTTATGTGAGGTCGCCATTCTCGGCATCCAGAGACTCTACTGCCCCTTCGTGGACTGCTCGGTGATGCTTATCAATGATGGAGGCCGAGTGTCTGAGCTGTAA

Protein sequence

MFTSNECSHFFCKDCISNHIAAKVEDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRWGDVLCEVAILGIQRLYCPFVDCSVMLINDGGRVSEL

Homology

[Close]

BLAST of HG10003420 vs. NCBI nr
Match: KAE8649011.1 (hypothetical protein Csa_009107 [Cucumis sativus])

HSP 1 Score: 161.0 bits (406), Expect = 5.1e-36
Identity = 69/95 (72.63%), Postives = 82/95 (86.32%), Query Frame = 0


Query: 1   MFTSNECSHFFCKDCISNHIAAKVEDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRWG 60
           MFT++ECSH FCKDCISNHIAAK+EDNIAN+KCP+PGCE VL P++CHSF+PKN+LDRWG
Sbjct: 34  MFTNHECSHSFCKDCISNHIAAKLEDNIANVKCPQPGCEAVLHPDVCHSFVPKNVLDRWG 93

Query: 61  DVLCEVAILGIQRL-YCPFVDCSVMLINDGGRVSE 95
            VLCE  ILG  RL YCPF+DCSV LI+DG   ++
Sbjct: 94  YVLCEAFILGNHRLIYCPFMDCSVALIDDGDEATK 128

[Close]

BLAST of HG10003420 vs. NCBI nr
Match: XP_004141646.1 (probable E3 ubiquitin-protein ligase ARI3 [Cucumis sativus])

HSP 1 Score: 161.0 bits (406), Expect = 5.1e-36
Identity = 69/95 (72.63%), Postives = 82/95 (86.32%), Query Frame = 0


Query: 1   MFTSNECSHFFCKDCISNHIAAKVEDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRWG 60
           MFT++ECSH FCKDCISNHIAAK+EDNIAN+KCP+PGCE VL P++CHSF+PKN+LDRWG
Sbjct: 23  MFTNHECSHSFCKDCISNHIAAKLEDNIANVKCPQPGCEAVLHPDVCHSFVPKNVLDRWG 82

Query: 61  DVLCEVAILGIQRL-YCPFVDCSVMLINDGGRVSE 95
            VLCE  ILG  RL YCPF+DCSV LI+DG   ++
Sbjct: 83  YVLCEAFILGNHRLIYCPFMDCSVALIDDGDEATK 117

[Close]

BLAST of HG10003420 vs. NCBI nr
Match: KAA0039247.1 (putative E3 ubiquitin-protein ligase RNF217 isoform X2 [Cucumis melo var. makuwa] >TYK00433.1 putative E3 ubiquitin-protein ligase RNF217 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 153.7 bits (387), Expect = 8.1e-34
Identity = 66/90 (73.33%), Postives = 78/90 (86.67%), Query Frame = 0


Query: 1   MFTSNECSHFFCKDCISNHIAAKVEDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRWG 60
           MFT++ECSH FCKDCIS+HIAAK+EDNIAN+KCP+  C+ +L P+ICHSF+PKNL DRWG
Sbjct: 23  MFTNHECSHSFCKDCISSHIAAKLEDNIANVKCPQTDCQALLHPDICHSFIPKNLFDRWG 82

Query: 61  DVLCEVAILGIQRL-YCPFVDCSVMLINDG 90
           DVLCE  ILG  RL YCPF+DCSV LI+DG
Sbjct: 83  DVLCEAFILGNHRLIYCPFMDCSVALIDDG 112

[Close]

BLAST of HG10003420 vs. NCBI nr
Match: XP_022152679.1 (E3 ubiquitin-protein ligase RNF144A-like [Momordica charantia])

HSP 1 Score: 144.4 bits (363), Expect = 4.9e-31
Identity = 61/93 (65.59%), Postives = 75/93 (80.65%), Query Frame = 0


Query: 1   MFTSNECSHFFCKDCISNHIAAKVEDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRWG 60
           MFTS+ CSH FC DCIS HIAAK+EDNIA +KCPEP CE+ L+P +C SF+P+ +L+RWG
Sbjct: 99  MFTSSACSHSFCADCISKHIAAKLEDNIA-VKCPEPKCESTLEPWMCDSFVPREVLERWG 158

Query: 61  DVLCEVAILGIQRLYCPFVDCSVMLINDGGRVS 94
           D LCE  ILGI R+YCPFVDCS  +I+DGG  +
Sbjct: 159 DALCEAIILGIPRMYCPFVDCSAAMIDDGGEAA 190

[Close]

BLAST of HG10003420 vs. NCBI nr
Match: XP_030968567.1 (E3 ubiquitin-protein ligase RNF144A-like [Quercus lobata])

HSP 1 Score: 131.0 bits (328), Expect = 5.6e-27
Identity = 49/92 (53.26%), Postives = 68/92 (73.91%), Query Frame = 0


Query: 1   MFTSNECSHFFCKDCISNHIAAKVEDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRWG 60
           MFT+N C+H FC  CIS ++A K++DNI+++KCP+P C  +L+P  C   +P+ + DRWG
Sbjct: 106 MFTNNSCTHSFCAGCISQYVATKLQDNISSVKCPDPKCRGLLEPQCCRDIIPQEVFDRWG 165

Query: 61  DVLCEVAILGIQRLYCPFVDCSVMLINDGGRV 93
           D LCE  ILG Q+ YCP+ DCS ML++DGG V
Sbjct: 166 DALCESLILGSQKFYCPYKDCSAMLVDDGGEV 197

[Close]

BLAST of HG10003420 vs. ExPASy Swiss-Prot
Match: A2VEA3 (E3 ubiquitin-protein ligase ARIH1 OS=Bos taurus OX=9913 GN=ARIH1 PE=2 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 6.9e-04
Identity = 25/84 (29.76%), Postives = 37/84 (44.05%), Query Frame = 0


Query: 2   FTSNECSHFFCKDCISNHIAAKV--EDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRW 61
           FT  EC H FC  C S ++  K+  E     I CP  GC+ ++D N     +  + +   
Sbjct: 196 FTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLK 255

Query: 62  GDVLCEVAILGIQRL--YCPFVDC 82
              L   + +   RL  +CP  DC
Sbjct: 256 YQHLITNSFVECNRLLKWCPAPDC 279

[Close]

BLAST of HG10003420 vs. ExPASy Swiss-Prot
Match: Q9Y4X5 (E3 ubiquitin-protein ligase ARIH1 OS=Homo sapiens OX=9606 GN=ARIH1 PE=1 SV=2)

HSP 1 Score: 44.7 bits (104), Expect = 6.9e-04
Identity = 25/84 (29.76%), Postives = 37/84 (44.05%), Query Frame = 0


Query: 2   FTSNECSHFFCKDCISNHIAAKV--EDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRW 61
           FT  EC H FC  C S ++  K+  E     I CP  GC+ ++D N     +  + +   
Sbjct: 198 FTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLK 257

Query: 62  GDVLCEVAILGIQRL--YCPFVDC 82
              L   + +   RL  +CP  DC
Sbjct: 258 YQHLITNSFVECNRLLKWCPAPDC 281

[Close]

BLAST of HG10003420 vs. ExPASy Swiss-Prot
Match: Q9Z1K5 (E3 ubiquitin-protein ligase ARIH1 OS=Mus musculus OX=10090 GN=Arih1 PE=1 SV=3)

HSP 1 Score: 44.7 bits (104), Expect = 6.9e-04
Identity = 25/84 (29.76%), Postives = 37/84 (44.05%), Query Frame = 0


Query: 2   FTSNECSHFFCKDCISNHIAAKV--EDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRW 61
           FT  EC H FC  C S ++  K+  E     I CP  GC+ ++D N     +  + +   
Sbjct: 196 FTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLK 255

Query: 62  GDVLCEVAILGIQRL--YCPFVDC 82
              L   + +   RL  +CP  DC
Sbjct: 256 YQHLITNSFVECNRLLKWCPAPDC 279

[Close]

BLAST of HG10003420 vs. ExPASy Swiss-Prot
Match: B1H1E4 (E3 ubiquitin-protein ligase arih1 OS=Xenopus tropicalis OX=8364 GN=arih1 PE=2 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 6.9e-04
Identity = 25/84 (29.76%), Postives = 37/84 (44.05%), Query Frame = 0


Query: 2   FTSNECSHFFCKDCISNHIAAKV--EDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRW 61
           FT  EC H FC  C S ++  K+  E     I CP  GC+ ++D N     +  + +   
Sbjct: 170 FTGLECGHKFCMQCWSEYLTTKIIEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLK 229

Query: 62  GDVLCEVAILGIQRL--YCPFVDC 82
              L   + +   RL  +CP  DC
Sbjct: 230 YQHLITNSFVECNRLLKWCPAPDC 253

[Close]

BLAST of HG10003420 vs. ExPASy TrEMBL
Match: A0A0A0KSV5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G650600 PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.4e-36
Identity = 69/95 (72.63%), Postives = 82/95 (86.32%), Query Frame = 0


Query: 1   MFTSNECSHFFCKDCISNHIAAKVEDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRWG 60
           MFT++ECSH FCKDCISNHIAAK+EDNIAN+KCP+PGCE VL P++CHSF+PKN+LDRWG
Sbjct: 23  MFTNHECSHSFCKDCISNHIAAKLEDNIANVKCPQPGCEAVLHPDVCHSFVPKNVLDRWG 82

Query: 61  DVLCEVAILGIQRL-YCPFVDCSVMLINDGGRVSE 95
            VLCE  ILG  RL YCPF+DCSV LI+DG   ++
Sbjct: 83  YVLCEAFILGNHRLIYCPFMDCSVALIDDGDEATK 117

[Close]

BLAST of HG10003420 vs. ExPASy TrEMBL
Match: A0A5A7TCD1 (Putative E3 ubiquitin-protein ligase RNF217 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00360 PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 3.9e-34
Identity = 66/90 (73.33%), Postives = 78/90 (86.67%), Query Frame = 0


Query: 1   MFTSNECSHFFCKDCISNHIAAKVEDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRWG 60
           MFT++ECSH FCKDCIS+HIAAK+EDNIAN+KCP+  C+ +L P+ICHSF+PKNL DRWG
Sbjct: 23  MFTNHECSHSFCKDCISSHIAAKLEDNIANVKCPQTDCQALLHPDICHSFIPKNLFDRWG 82

Query: 61  DVLCEVAILGIQRL-YCPFVDCSVMLINDG 90
           DVLCE  ILG  RL YCPF+DCSV LI+DG
Sbjct: 83  DVLCEAFILGNHRLIYCPFMDCSVALIDDG 112

[Close]

BLAST of HG10003420 vs. ExPASy TrEMBL
Match: A0A6J1DGR7 (E3 ubiquitin-protein ligase RNF144A-like OS=Momordica charantia OX=3673 GN=LOC111020341 PE=3 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.4e-31
Identity = 61/93 (65.59%), Postives = 75/93 (80.65%), Query Frame = 0


Query: 1   MFTSNECSHFFCKDCISNHIAAKVEDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRWG 60
           MFTS+ CSH FC DCIS HIAAK+EDNIA +KCPEP CE+ L+P +C SF+P+ +L+RWG
Sbjct: 99  MFTSSACSHSFCADCISKHIAAKLEDNIA-VKCPEPKCESTLEPWMCDSFVPREVLERWG 158

Query: 61  DVLCEVAILGIQRLYCPFVDCSVMLINDGGRVS 94
           D LCE  ILGI R+YCPFVDCS  +I+DGG  +
Sbjct: 159 DALCEAIILGIPRMYCPFVDCSAAMIDDGGEAA 190

[Close]

BLAST of HG10003420 vs. ExPASy TrEMBL
Match: A0A7N2LM99 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.7e-27
Identity = 49/92 (53.26%), Postives = 68/92 (73.91%), Query Frame = 0


Query: 1   MFTSNECSHFFCKDCISNHIAAKVEDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRWG 60
           MFT+N C+H FC  CIS ++A K++DNI+++KCP+P C  +L+P  C   +P+ + DRWG
Sbjct: 106 MFTNNSCTHSFCAGCISQYVATKLQDNISSVKCPDPKCRGLLEPQCCRDIIPQEVFDRWG 165

Query: 61  DVLCEVAILGIQRLYCPFVDCSVMLINDGGRV 93
           D LCE  ILG Q+ YCP+ DCS ML++DGG V
Sbjct: 166 DALCESLILGSQKFYCPYKDCSAMLVDDGGEV 197

[Close]

BLAST of HG10003420 vs. ExPASy TrEMBL
Match: A0A7N2R445 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.7e-27
Identity = 49/92 (53.26%), Postives = 68/92 (73.91%), Query Frame = 0


Query: 1   MFTSNECSHFFCKDCISNHIAAKVEDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRWG 60
           MFT+N C+H FC  CIS ++A K++DNI+++KCP+P C  +L+P  C   +P+ + DRWG
Sbjct: 106 MFTNNSCTHSFCAGCISQYVATKLQDNISSVKCPDPKCRGLLEPQCCRDIIPQEVFDRWG 165

Query: 61  DVLCEVAILGIQRLYCPFVDCSVMLINDGGRV 93
           D LCE  ILG Q+ YCP+ DCS ML++DGG V
Sbjct: 166 DALCESLILGSQKFYCPYKDCSAMLVDDGGEV 197

[Close]

BLAST of HG10003420 vs. TAIR 10
Match: AT3G53690.1 (RING/U-box superfamily protein )

HSP 1 Score: 110.5 bits (275), Expect = 7.3e-25
Identity = 42/91 (46.15%), Postives = 60/91 (65.93%), Query Frame = 0


Query: 2   FTSNECSHFFCKDCISNHIAAKVEDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRWGD 61
           F    CSHF+C DC+S +IAAK++DNI +I+CP  GC   L+P+ C   LPK + DRWGD
Sbjct: 128 FRIGGCSHFYCNDCVSKYIAAKLQDNILSIECPVSGCSGRLEPDQCRQILPKEVFDRWGD 187

Query: 62  VLCEVAILGIQRLYCPFVDCSVMLINDGGRV 93
            LCE  ++  ++ YCP+ DCS ++  +   V
Sbjct: 188 ALCEAVVMRSKKFYCPYKDCSALVFLEESEV 218

[Close]

BLAST of HG10003420 vs. TAIR 10
Match: AT3G14250.1 (RING/U-box superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 1.9e-20
Identity = 33/85 (38.82%), Postives = 54/85 (63.53%), Query Frame = 0


Query: 4   SNECSHFFCKDCISNHIAAKVEDNIANIKCPEPGCETVLDPNICHSFLPKNLLDRWGDVL 63
           +  C+H +C DC   ++A K+++N + IKCP+  C  +++P  C   +PK++ DRW  +L
Sbjct: 111 TTNCTHAYCTDCTVRYVATKIKENASRIKCPDVECTRLIEPYTCRDLIPKDVFDRWEKIL 170

Query: 64  CEVAILGIQRLYCPFVDCSVMLIND 89
           CE  I    + YCPF DCS M++N+
Sbjct: 171 CESLISSWDKFYCPFKDCSAMMVNN 195

The following BLAST results are available for this feature:

BLAST of HG10003420 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8649011.1	5.1e-36	72.63	hypothetical protein Csa_009107 [Cucumis sativus]	[more]
XP_004141646.1	5.1e-36	72.63	probable E3 ubiquitin-protein ligase ARI3 [Cucumis sativus]	[more]
KAA0039247.1	8.1e-34	73.33	putative E3 ubiquitin-protein ligase RNF217 isoform X2 [Cucumis melo var. makuwa...	[more]
XP_022152679.1	4.9e-31	65.59	E3 ubiquitin-protein ligase RNF144A-like [Momordica charantia]	[more]
XP_030968567.1	5.6e-27	53.26	E3 ubiquitin-protein ligase RNF144A-like [Quercus lobata]	[more]

BLAST of HG10003420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A2VEA3	6.9e-04	29.76	E3 ubiquitin-protein ligase ARIH1 OS=Bos taurus OX=9913 GN=ARIH1 PE=2 SV=1	[more]
Q9Y4X5	6.9e-04	29.76	E3 ubiquitin-protein ligase ARIH1 OS=Homo sapiens OX=9606 GN=ARIH1 PE=1 SV=2	[more]
Q9Z1K5	6.9e-04	29.76	E3 ubiquitin-protein ligase ARIH1 OS=Mus musculus OX=10090 GN=Arih1 PE=1 SV=3	[more]
B1H1E4	6.9e-04	29.76	E3 ubiquitin-protein ligase arih1 OS=Xenopus tropicalis OX=8364 GN=arih1 PE=2 SV...	[more]

BLAST of HG10003420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KSV5	2.4e-36	72.63	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G650600 PE=3 SV=1	[more]
A0A5A7TCD1	3.9e-34	73.33	Putative E3 ubiquitin-protein ligase RNF217 isoform X2 OS=Cucumis melo var. maku...	[more]
A0A6J1DGR7	2.4e-31	65.59	E3 ubiquitin-protein ligase RNF144A-like OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A7N2LM99	2.7e-27	53.26	Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1	[more]
A0A7N2R445	2.7e-27	53.26	Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1	[more]

BLAST of HG10003420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53690.1	7.3e-25	46.15	RING/U-box superfamily protein	[more]
AT3G14250.1	1.9e-20	38.82	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..70 e-value: 2.3E-18 score: 67.9
None	No IPR available	PANTHER	PTHR11685:SF309	RBR-TYPE E3 UBIQUITIN TRANSFERASE	coord: 2..93
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 2..65
IPR031127	E3 ubiquitin ligase RBR family	PANTHER	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING	coord: 2..93
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 7..16

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10003420.1	HG10003420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity