HG10003281 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10003281
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	subtilisin-like protease SBT5.3
Location	Chr11: 19668878 .. 19674332 (+)
RNA-Seq Expression	HG10003281
Synteny	HG10003281

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGCCATTTTGTTGAAGGTGCTAATATATTTGGCTTTGGTAATGGCACTGCAAAAGGAGGTTCCCCTAAAGCCCTTGTTGCTGCCTATAAAGTTTGTTGGCCTCAATTGTTCTTGGTTGGTGAATGTTTTGAGGCAGACATTCTAGCTGGCTTTGAAGCTGCCATTAGTGATGGAGTTGATGTTCTGTCTGTTTCACTCGGTGGAGATTCTCAAGATTTTGCACAAGATTCAATAGCTATAGGAGCTTTTCATGCAGTTCAGAATGGTATTACTGTTGTTTGTTCTGCTGGAAACTCTGGACCAACTCCTGGCTCTGTTTCTAATGTTGCACCATGGATTATAACTGTGGGAGCTAGCACAATTGATAGGCTTTTTACGAGTTATGTCGCGCTCGGAGACAAGAGGCACATCAAGGTAATCTTCTAAGAAACACATACACTTTTGTTTAATTTGGTTCGCTAACGTTGATGTTATGAAATCAACAAATAATGATGAGTAGAAAATCAAAGAACTTTACGTGTAAACAGTAGAGAATCGACATAGAGATTTACATGTGGTTCACTAACAATATGTTAGCTACATGCACACACAAAGAGAGAGAAGTTTATTATTAGAAAGAATAATTCAGATTACAAATCAGTGGTGCCTTTAGAGTTAGAGAGTTTATATAGTGCACTTCTCTAAATTCTATGTAAAAAATTTCTACTACTACCGAAATTAGGAAACACAAGAATTTATGTGAAAAACTACAAACCTAGAGAAAAACCACGACCCATTAGAAGAAATCCAATATGTGAAAAATTATTACAAACACATAGAATTCTCTCCTCGATGACAATTATTACAAGAACACTCTCTCAAATCTTATATATTATTCACACCCTTTTTTTCAATCTCAAACGAGAGCATACAAAGCGAAAAATTTAATTAGAGTTAATACACCTAATTTAAAATGTTACTATCTGGGACAATTTGAAACCAAACTCATATACTCTTTTTTATAGTGTTTGGACCTCATTGTACAAATTTTATATTCGGACGACACTTATTTAGCATACTAAATAGACATAATAATGTAGGACAAAAACAACAAATTTCGAAAGCAAACATATATCGAACTCATTTTTCTAAGCGCATAAATACATAAACTCATTTGACTTTGAATTTTGAATTTCCGTATAAAGTTATATATATATATATATATATATACACTTTTTTTTTAATAAACACATTCTTGTCATCTTCATATTGTGTTGTATTTGTATTTTATATATATCCGTATCCGTGCTTCTTTGGTAATCATCTTAATTTAATTTGAGTATATTGCTCTATCCATGTTTTTGCTGTCTTTAATGATTTTCATTATTATCAATATCAGGGTGCAAGTCTTTCTGATAAAATATTGCCAGAACAGAAATTCTATCCATTGATCAATGCTGTAGATGCAAAAGCCAACAATGTCTCTGAAGACTACGCGTGAGTATATTTTTGTTTAAATTATATAAAATATACTCCCGAATTTTCGAGTAGGTTTCAATTATATTATTAAACATTTAATAATTTTATTTGATTAACTTTTAAACTTTGAAATTGTTCCAAAAACTATCTTTAATCGAGGTTTTTTTTTTCATTAAAACCACGAATGAAAATTATTTAGTGTTAGTTGATATATAAAAATTGGAATGAATACACATGACCATATGATCATTTACGATATCATTCATGTAAATAGACAATGGTATAAGTTCGTGTAAAAATAAAAAATTAAAAATTAAAAGAATTGATAATGTTGCCATCGAATGATGTTTTAAATTATTATCAATGATGTCTTTCGAGTTGAAATGAATGGTAAAAACCAAAATTAGTGCATGTCTAGAAATGATTTTGAAATGGATAAAATCACTTTTGTTATATTGAAACACTTTTAATCACTCAAAATAATTCAATGTTTAATTTTACACTTTCAAACACACTTTTCATACTATTCAAATTGATTTTGAATGATTAAAAACATTTTTCAAGTGACTTCAGAATATCATTCCCAAACATGTAAGTAATTTTCTTTATAGTTGAAAATGAGTTGTAAATGATACTATGTACGTCTGTCAATTTTTACAATTAGTAGCTCCGGTTAATTTCTATCCTCCATTTTAATGAAAAAACAAAGCCTTCTGCTTTTTATTTATACGTTTTTTCACATCTGCTCCCGCAGAATCGTCTTGAAGATATTTTTTGTGGTTTGAGAGAACCGAGTCAAGATCCTCTTCGGCCACAGATTCCTTTTCTCCTTTATTTCCATTTTTTTCCTTCGATATAAAAAAAGATCTTTTTTTCTCTCCCATGAGTTTTTTTACTTTTTCCCATTCTCAAAATGAAACTTTTCAAAAGTCTAAAGTTATAATTAAAGTTGAAGATAATTTTGTACAATTTAACCCAATGAAATTTTGATGGTAATAAGAATAAAATGACATTTAATTAAAAAAAATTAAAATAGAACAACAAAATTTTTAGATAGTTGTGATGGACGCTCTATTAGTGATAGAAATGATAGAAATCTATCACTGATAGATGCTGATATAAGTCTATCAATGTATATCAGTATTTTTTTTTATTATTTTCTGTAAATAGTTTGATATTTTTTCTATCCGTAAAAATTTGTCTTTAATGAAGCAAATTTGAATGTGTGTGTTATTGTAGCCTACTATGTGAAGAAGGGTCTCTTGATCCTAAGAAGGCAAAAGGGAAGATTGTAATTTGCCTTAGAGGGGACAATGCAAGAGTGGATAAAGGTTTTGTGGCTGCTAAAGCAGGTGCTGTTGGAATGATTCTTGCTAATGACAAGGAAAATGGAGATGAACTTTTAGCTGATGCTCATTTCCTTCCTGCTTCCCATATAACCTATTCTGATGGTCAATTAGTCTACCAATACATCAACTCTACCAAGTAATTTTACTCCTTTTTCTTCCCTCAAACTCCCATTTTTATATTCATTAAACAAACGTGTTGCTTTTAAAATTATTACACGTCTAATTCTGTGTCTGTTTAGTCACTCAACTTTAGAAAGCGTCTAATAGATTATCTAATTATAAACTTTCATTTTTGGGTCTAGTAGCTTCTTAGACTTTGAACCTTTGTGTCTAATCATAAGTTTTGGAACTTTAAAATGTTGTCTCATTAGACACAAGAGCTAACAGTTTAGGATCTTATTAGCCGCCGTCAAATAAATCAATTGGTTTAATTTTGTTTTAAAAGTATATCAAGAACATTATTGAATACAAAATCAAAAGTTTTAAGGACTTACTATGCACTTTAAAAGTTGATGATCTATTAAATGCAAACTTAGAGGTTTAGGCCTTGTTTGATAACAATTTGGTTTTTGAAAATTAAGCTTATAAACATTTACTTCCATTTATTGATTTCTTCGTTTTTTTTTTTTTATTTACTTTAATTTTAGGATGTAGCCAAAAGTCAATACTAATTTTGAAAACTAACAAAAAAAATGGTATTTTTAGGCCTTTGGTTGATAATCATTTGATTTTTAGTTTGTAGCTATTGAAAATTATGTTTGCTTTCTCACAATTTTATAAGGGGACTTTTAAAAATAGAAAAATAAAAGAAACTATTTACACAAAATAGCAAAATTTTTAGATAGTTGGATAGATGATGAAAGAAGTCTATCAGAGCCTATCGGTGATAGAAATGATAGAAGTCTATCACAGATAGATGCTGATAGAAGTCTATCAATGTTTATCAGTGATTTTTTGTTATTTTCTGTAAATAGTTTGATATTTTTTCTATTTGTGAAAATTTTCTATTTTATCATCATGATTTTTATCTTCTTTTAGTTAAGTTAATAATTGAATTTTTAACTCAATTATAAAAGAAAAAAAAAAGTTTTTAAAAACTATTATAAAGAAAAGACTTAGCTTAGATTTTGAAAACACTCCTAACATGTAAACAGCAAACCAAAGAAATCAATAGGCGGTGTAGGTAGTGTTGATAGGCTTCATTTTCAAAAACTAAAAAGTTATCAAATGGGGGCTTAAAAAACTTATCTTTGTTTTTAAACTTTGGCTAAGAATCCAAATATTGTTTAATTAGGAAAGATGAAAACCATGGTAAGAAAGTCGTAAGAAAATAAACACAAATTTTAAAAACTAAATAGGACATTAGGGGACCATTAGACAACAAATGTTCAGAGATTCATTAGACACGAACTTAAAAGTTCAAGTACGAAAAAACTCGTAGAACTCATACAACAAATTTTGTGCTTGAACTAGAATTCCAATGGCTTACATGACACACGTGAGGACAGAGTTGGGAGTCAAACCAGCACCAGTTATGGCTTCATTCTCGTCAAGAGGTCCCAACACAATTGATCCTTCAATACTCAAGGTTAGTTCTTCAATGGTTGAAATGTCTTGAATCTGTTATATAGCGCTTTGAATGATCCAGCACCGTTATGTCATGTACTAACACACCGTTAATAAACCACATAAATCTGTTTATCAATTTTGTTGTTTGATTCTTTGGTTGTCGATTGTTTCTATCAGAACAAACTTACGGTACGAAATTCGGCTTGCATTATTGTTTAAAAATTTTTGCAGCCTGATATAACAGCACCAGGTGTGAATATATTAGCAGCTTACTCAGAAGATACATCACCAACAGGTTCAAGTTTAGATAAACGTCGAGTGGCATTTAATGTAGATTCTGGAACTTCCATGTCATGCCCTCATATTTCTGGAATTGTTGGCCTTCTCAAAACCCTTTATCCTAAATGGAGTCCTGCAGCTATTAGATCGGCAATCATGACCACAGGTACATTATTTTTCTCCCCTTTCATTATTATTTACTTTTTTTGAAATCATTAACAAATATTTTACTTAAATTAATCTTGGCAGCTGGAACCAAAGCCAATGACTTGAATCCAATACTAAGCACAAACCAAGTGAAAGCAAATGCATTAGAATATGGAGCAGGCCATGTTCGTCCAAACAAAGCAGCAAATCCAGGCCTTGTTTATGACCTTTCCACCAAAGACTACTTGAATTACCTATGTGCTCGAGGCTACAACAAAACACAAATCAAACAATTCTCCAATGACACTTCATTTGTTTGTTCAAAATCATTCAAAATAACAGATTTGAACTACCCGTCGATCTCGATTCCGGAGCTGAAATCGGAGGCCGTCGTGAAGATCAAAAGGCGAGTGAAGAATGTGGGAAGTCCAGGCACGTATGTCGTTCAAGTCAACGCGCCGCCGGGGGTTTCGGTTTCGGTTGAGCCAACTAGTTTGAAGTTCACTGGAATTGATGAAGAGAAAAGTTTTAGAGTTGTGGTGAAGAGTAGTGTGCCTAATGATTTTCCTCGAAAGTATGTGTTTGGGAGACTTGAATGGTCTGACGGGATCCATCGTGTTAGAAGTCCGATTGTAGTGAGATTAGGCGGATGA

mRNA sequence

GGCCATTTTGTTGAAGGTGCTAATATATTTGGCTTTGGTAATGGCACTGCAAAAGGAGGTTCCCCTAAAGCCCTTGTTGCTGCCTATAAAGTTTGTTGGCCTCAATTGTTCTTGGTTGGTGAATGTTTTGAGGCAGACATTCTAGCTGGCTTTGAAGCTGCCATTAGTGATGGAGTTGATGTTCTGTCTGTTTCACTCGGTGGAGATTCTCAAGATTTTGCACAAGATTCAATAGCTATAGGAGCTTTTCATGCAGTTCAGAATGGTATTACTGTTGTTTGTTCTGCTGGAAACTCTGGACCAACTCCTGGCTCTGTTTCTAATGTTGCACCATGGATTATAACTGTGGGAGCTAGCACAATTGATAGGCTTTTTACGAGTTATGTCGCGCTCGGAGACAAGAGGCACATCAAGGGTGCAAGTCTTTCTGATAAAATATTGCCAGAACAGAAATTCTATCCATTGATCAATGCTGTAGATGCAAAAGCCAACAATGTCTCTGAAGACTACGCCCTACTATGTGAAGAAGGGTCTCTTGATCCTAAGAAGGCAAAAGGGAAGATTGTAATTTGCCTTAGAGGGGACAATGCAAGAGTGGATAAAGGTTTTGTGGCTGCTAAAGCAGGTGCTGTTGGAATGATTCTTGCTAATGACAAGGAAAATGGAGATGAACTTTTAGCTGATGCTCATTTCCTTCCTGCTTCCCATATAACCTATTCTGATGGTCAATTAGTCTACCAATACATCAACTCTACCAAAATTCCAATGGCTTACATGACACACGTGAGGACAGAGTTGGGAGTCAAACCAGCACCAGTTATGGCTTCATTCTCGTCAAGAGGTCCCAACACAATTGATCCTTCAATACTCAAGCCTGATATAACAGCACCAGGTGTGAATATATTAGCAGCTTACTCAGAAGATACATCACCAACAGGTTCAAGTTTAGATAAACGTCGAGTGGCATTTAATGTAGATTCTGGAACTTCCATGTCATGCCCTCATATTTCTGGAATTGTTGGCCTTCTCAAAACCCTTTATCCTAAATGGAGTCCTGCAGCTATTAGATCGGCAATCATGACCACAGCTGGAACCAAAGCCAATGACTTGAATCCAATACTAAGCACAAACCAAGTGAAAGCAAATGCATTAGAATATGGAGCAGGCCATGTTCGTCCAAACAAAGCAGCAAATCCAGGCCTTGTTTATGACCTTTCCACCAAAGACTACTTGAATTACCTATGTGCTCGAGGCTACAACAAAACACAAATCAAACAATTCTCCAATGACACTTCATTTGTTTGTTCAAAATCATTCAAAATAACAGATTTGAACTACCCGTCGATCTCGATTCCGGAGCTGAAATCGGAGGCCGTCGTGAAGATCAAAAGGCGAGTGAAGAATGTGGGAAGTCCAGGCACGTATGTCGTTCAAGTCAACGCGCCGCCGGGGGTTTCGGTTTCGGTTGAGCCAACTAGTTTGAAGTTCACTGGAATTGATGAAGAGAAAAGTTTTAGAGTTGTGGTGAAGAGTAGTGTGCCTAATGATTTTCCTCGAAAGTATGTGTTTGGGAGACTTGAATGGTCTGACGGGATCCATCGTGTTAGAAGTCCGATTGTAGTGAGATTAGGCGGATGA

Coding sequence (CDS)

GGCCATTTTGTTGAAGGTGCTAATATATTTGGCTTTGGTAATGGCACTGCAAAAGGAGGTTCCCCTAAAGCCCTTGTTGCTGCCTATAAAGTTTGTTGGCCTCAATTGTTCTTGGTTGGTGAATGTTTTGAGGCAGACATTCTAGCTGGCTTTGAAGCTGCCATTAGTGATGGAGTTGATGTTCTGTCTGTTTCACTCGGTGGAGATTCTCAAGATTTTGCACAAGATTCAATAGCTATAGGAGCTTTTCATGCAGTTCAGAATGGTATTACTGTTGTTTGTTCTGCTGGAAACTCTGGACCAACTCCTGGCTCTGTTTCTAATGTTGCACCATGGATTATAACTGTGGGAGCTAGCACAATTGATAGGCTTTTTACGAGTTATGTCGCGCTCGGAGACAAGAGGCACATCAAGGGTGCAAGTCTTTCTGATAAAATATTGCCAGAACAGAAATTCTATCCATTGATCAATGCTGTAGATGCAAAAGCCAACAATGTCTCTGAAGACTACGCCCTACTATGTGAAGAAGGGTCTCTTGATCCTAAGAAGGCAAAAGGGAAGATTGTAATTTGCCTTAGAGGGGACAATGCAAGAGTGGATAAAGGTTTTGTGGCTGCTAAAGCAGGTGCTGTTGGAATGATTCTTGCTAATGACAAGGAAAATGGAGATGAACTTTTAGCTGATGCTCATTTCCTTCCTGCTTCCCATATAACCTATTCTGATGGTCAATTAGTCTACCAATACATCAACTCTACCAAAATTCCAATGGCTTACATGACACACGTGAGGACAGAGTTGGGAGTCAAACCAGCACCAGTTATGGCTTCATTCTCGTCAAGAGGTCCCAACACAATTGATCCTTCAATACTCAAGCCTGATATAACAGCACCAGGTGTGAATATATTAGCAGCTTACTCAGAAGATACATCACCAACAGGTTCAAGTTTAGATAAACGTCGAGTGGCATTTAATGTAGATTCTGGAACTTCCATGTCATGCCCTCATATTTCTGGAATTGTTGGCCTTCTCAAAACCCTTTATCCTAAATGGAGTCCTGCAGCTATTAGATCGGCAATCATGACCACAGCTGGAACCAAAGCCAATGACTTGAATCCAATACTAAGCACAAACCAAGTGAAAGCAAATGCATTAGAATATGGAGCAGGCCATGTTCGTCCAAACAAAGCAGCAAATCCAGGCCTTGTTTATGACCTTTCCACCAAAGACTACTTGAATTACCTATGTGCTCGAGGCTACAACAAAACACAAATCAAACAATTCTCCAATGACACTTCATTTGTTTGTTCAAAATCATTCAAAATAACAGATTTGAACTACCCGTCGATCTCGATTCCGGAGCTGAAATCGGAGGCCGTCGTGAAGATCAAAAGGCGAGTGAAGAATGTGGGAAGTCCAGGCACGTATGTCGTTCAAGTCAACGCGCCGCCGGGGGTTTCGGTTTCGGTTGAGCCAACTAGTTTGAAGTTCACTGGAATTGATGAAGAGAAAAGTTTTAGAGTTGTGGTGAAGAGTAGTGTGCCTAATGATTTTCCTCGAAAGTATGTGTTTGGGAGACTTGAATGGTCTGACGGGATCCATCGTGTTAGAAGTCCGATTGTAGTGAGATTAGGCGGATGA

Protein sequence

GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVDVLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGASTIDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLDPKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYNKTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDGIHRVRSPIVVRLGG

Homology

[Close]

BLAST of HG10003281 vs. NCBI nr
Match: XP_038904154.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 1008.4 bits (2606), Expect = 2.3e-290
Identity = 503/545 (92.29%), Postives = 524/545 (96.15%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           GHFVEGANIFG+GNGTAKGGSPKALVAAYKVCWPQ+FL GECFEADILAGFEAAISDGVD
Sbjct: 229 GHFVEGANIFGYGNGTAKGGSPKALVAAYKVCWPQVFLFGECFEADILAGFEAAISDGVD 288

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           VLSVSLGGD  DFAQDSI+IGAFHAVQNGITVVCSAGNSGP PGSVSNVAPWIITVGAST
Sbjct: 289 VLSVSLGGDPSDFAQDSISIGAFHAVQNGITVVCSAGNSGPIPGSVSNVAPWIITVGAST 348

Query: 121 IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
           IDRLFTSYVALGDK+HIKGASLSDKILPEQKFYPLI+++DAKANNVS  +AL+CEEGSLD
Sbjct: 349 IDRLFTSYVALGDKKHIKGASLSDKILPEQKFYPLISSLDAKANNVSNYHALICEEGSLD 408

Query: 181 PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
           PKK KGKIV+CLRG NARVDKGFVAA+AGAVGMILANDKENGDELLADAH LPASHITYS
Sbjct: 409 PKKVKGKIVVCLRGINARVDKGFVAAEAGAVGMILANDKENGDELLADAHLLPASHITYS 468

Query: 241 DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
           DGQLVYQYINSTKIPMAY+THVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN
Sbjct: 469 DGQLVYQYINSTKIPMAYLTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 528

Query: 301 ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
           ILAAYSED SP+GSS DKRRVAFNV+SGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM
Sbjct: 529 ILAAYSEDASPSGSSFDKRRVAFNVESGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 588

Query: 361 TTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYN 420
           TTAGTKANDLNPILST Q KANA  YGAGHVRPNKAANPGLVYDLSTKDYLNYLCA GYN
Sbjct: 589 TTAGTKANDLNPILSTKQEKANAFAYGAGHVRPNKAANPGLVYDLSTKDYLNYLCALGYN 648

Query: 421 KTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVN 480
           KTQIKQFSNDTSFVCSKSFK TDLNYPSISIP+LKSEAVVKIKRR+KNVGSPGTYVVQVN
Sbjct: 649 KTQIKQFSNDTSFVCSKSFKTTDLNYPSISIPDLKSEAVVKIKRRLKNVGSPGTYVVQVN 708

Query: 481 APPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDGIHRVRSPIV 540
           APPGVSV VEPTSLKFTGIDEEKSFRVV+KSSVPNDF + YVFGR+EWSDG HRV+SPIV
Sbjct: 709 APPGVSVFVEPTSLKFTGIDEEKSFRVVLKSSVPNDFRQMYVFGRIEWSDGNHRVKSPIV 768

Query: 541 VRLGG 546
           VR+GG
Sbjct: 769 VRVGG 773

[Close]

BLAST of HG10003281 vs. NCBI nr
Match: KAE8650095.1 (hypothetical protein Csa_011146 [Cucumis sativus])

HSP 1 Score: 929.5 bits (2401), Expect = 1.3e-266
Identity = 460/545 (84.40%), Postives = 496/545 (91.01%), Query Frame = 0


Query: 1    GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
            GHFV  AN+FG+GNGTAKGGSPKALVAAYKVCWPQ+   GECF+ADILAGFEAAI DGVD
Sbjct: 1659 GHFVPNANLFGYGNGTAKGGSPKALVAAYKVCWPQVLFFGECFDADILAGFEAAIGDGVD 1718

Query: 61   VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
            VLSVSLGG   DFA+DSI+IG+FHAVQNGI VVCSAGNSGPTPGSVSNVAPWIITVGAST
Sbjct: 1719 VLSVSLGGSPSDFAKDSISIGSFHAVQNGIVVVCSAGNSGPTPGSVSNVAPWIITVGAST 1778

Query: 121  IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
             DRL+TSYVA+GDKRH KGAS+SDK LP QKFYPLI+++DAKA NV+++ ALLCEEGSLD
Sbjct: 1779 TDRLYTSYVAIGDKRHFKGASVSDKKLPVQKFYPLISSLDAKAKNVTDNDALLCEEGSLD 1838

Query: 181  PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
            PKK  GKI+ICLRGDNARV KG+VAAKAGAVGMILAN +ENGDE+LADAH LPASHITYS
Sbjct: 1839 PKKVNGKIIICLRGDNARVAKGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYS 1898

Query: 241  DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
            DGQLVYQYINSTKIPMAYMTHVRTE G+KPAPVMASFSSRGPNT+DPSILKPDITAPG N
Sbjct: 1899 DGQLVYQYINSTKIPMAYMTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGEN 1958

Query: 301  ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
            ILAAYS D SPTG+  DKRRV FNV+SGTSMSCPH+SGIVGLLKTLYPKWSPAAIRSAIM
Sbjct: 1959 ILAAYSRDASPTGTDFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIM 2018

Query: 361  TTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYN 420
            TTAGTKANDL PILSTNQ KANA  YGAGHV PN+AA+PGLVYDLSTKDYLNYLCA+GYN
Sbjct: 2019 TTAGTKANDLTPILSTNQEKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYN 2078

Query: 421  KTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVN 480
              QIKQFSNDTSFVCSKSFK+TDLNYPSISIP L+ +  VKIKR++KNVGSPGTYVVQV 
Sbjct: 2079 TAQIKQFSNDTSFVCSKSFKLTDLNYPSISIPILEYDVAVKIKRKLKNVGSPGTYVVQVK 2138

Query: 481  APPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDGIHRVRSPIV 540
             P GVSVSVEPTSLKFTGIDEEKSFRVV+KS  PN    KY+FG+LEWSDG HRVRSPIV
Sbjct: 2139 EPLGVSVSVEPTSLKFTGIDEEKSFRVVLKSYEPNGSGPKYLFGKLEWSDGKHRVRSPIV 2198

Query: 541  VRLGG 546
            VRLGG
Sbjct: 2199 VRLGG 2203

[Close]

BLAST of HG10003281 vs. NCBI nr
Match: XP_011650462.2 (subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 929.5 bits (2401), Expect = 1.3e-266
Identity = 460/545 (84.40%), Postives = 496/545 (91.01%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           GHFV  AN+FG+GNGTAKGGSPKALVAAYKVCWPQ+   GECF+ADILAGFEAAI DGVD
Sbjct: 221 GHFVPNANLFGYGNGTAKGGSPKALVAAYKVCWPQVLFFGECFDADILAGFEAAIGDGVD 280

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           VLSVSLGG   DFA+DSI+IG+FHAVQNGI VVCSAGNSGPTPGSVSNVAPWIITVGAST
Sbjct: 281 VLSVSLGGSPSDFAKDSISIGSFHAVQNGIVVVCSAGNSGPTPGSVSNVAPWIITVGAST 340

Query: 121 IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
            DRL+TSYVA+GDKRH KGAS+SDK LP QKFYPLI+++DAKA NV+++ ALLCEEGSLD
Sbjct: 341 TDRLYTSYVAIGDKRHFKGASVSDKKLPVQKFYPLISSLDAKAKNVTDNDALLCEEGSLD 400

Query: 181 PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
           PKK  GKI+ICLRGDNARV KG+VAAKAGAVGMILAN +ENGDE+LADAH LPASHITYS
Sbjct: 401 PKKVNGKIIICLRGDNARVAKGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYS 460

Query: 241 DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
           DGQLVYQYINSTKIPMAYMTHVRTE G+KPAPVMASFSSRGPNT+DPSILKPDITAPG N
Sbjct: 461 DGQLVYQYINSTKIPMAYMTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGEN 520

Query: 301 ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
           ILAAYS D SPTG+  DKRRV FNV+SGTSMSCPH+SGIVGLLKTLYPKWSPAAIRSAIM
Sbjct: 521 ILAAYSRDASPTGTDFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIM 580

Query: 361 TTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYN 420
           TTAGTKANDL PILSTNQ KANA  YGAGHV PN+AA+PGLVYDLSTKDYLNYLCA+GYN
Sbjct: 581 TTAGTKANDLTPILSTNQEKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYN 640

Query: 421 KTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVN 480
             QIKQFSNDTSFVCSKSFK+TDLNYPSISIP L+ +  VKIKR++KNVGSPGTYVVQV 
Sbjct: 641 TAQIKQFSNDTSFVCSKSFKLTDLNYPSISIPILEYDVAVKIKRKLKNVGSPGTYVVQVK 700

Query: 481 APPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDGIHRVRSPIV 540
            P GVSVSVEPTSLKFTGIDEEKSFRVV+KS  PN    KY+FG+LEWSDG HRVRSPIV
Sbjct: 701 EPLGVSVSVEPTSLKFTGIDEEKSFRVVLKSYEPNGSGPKYLFGKLEWSDGKHRVRSPIV 760

Query: 541 VRLGG 546
           VRLGG
Sbjct: 761 VRLGG 765

[Close]

BLAST of HG10003281 vs. NCBI nr
Match: XP_016900594.1 (PREDICTED: subtilisin-like protease SBT5.3, partial [Cucumis melo])

HSP 1 Score: 912.1 bits (2356), Expect = 2.2e-261
Identity = 455/545 (83.49%), Postives = 493/545 (90.46%), Query Frame = 0


Query: 1    GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
            G+FV  AN+FG+GNGTAKGGSPKALVAAYKVCWP+L+L GECF+ADILAGFEAAI+DGVD
Sbjct: 960  GNFVPNANLFGYGNGTAKGGSPKALVAAYKVCWPKLWLFGECFDADILAGFEAAIADGVD 1019

Query: 61   VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
            VLSVSLGGD  DF QDSIAIG+FHAVQNGITVVCSAGNSGP PG+VSNVAPWIITVGAST
Sbjct: 1020 VLSVSLGGDPSDFGQDSIAIGSFHAVQNGITVVCSAGNSGPAPGTVSNVAPWIITVGAST 1079

Query: 121  IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
             DRL+T+YVA+GDKRH KGASLS+K LP QKFYPLIN+VDAK  N S  YA LCE  SLD
Sbjct: 1080 ADRLYTNYVAIGDKRHFKGASLSEKGLPVQKFYPLINSVDAKVQNFSNKYAQLCEIESLD 1139

Query: 181  PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
            P+K KGKIV+CLRGDNAR +KG+V AKAG VGMILAN +ENGD++ ADAH LPASHITYS
Sbjct: 1140 PEKVKGKIVVCLRGDNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYS 1199

Query: 241  DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
            DGQLVYQYINSTKIPMAY+TH RTE GVKPAP+MASFSSRGPN+IDPSILKPDITAPG N
Sbjct: 1200 DGQLVYQYINSTKIPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGEN 1259

Query: 301  ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
            ILAAYSED SP+G   DKRRV FNV+SGTSMSCPH+SGIVGLLKTLYPKWSPAAIRSAIM
Sbjct: 1260 ILAAYSEDGSPSGLYFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIM 1319

Query: 361  TTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYN 420
            TTAGTKANDLNPILST Q KAN   YGAGHVRPNKAA+PGLVYDLST+DYLNYLCA GY 
Sbjct: 1320 TTAGTKANDLNPILSTKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYK 1379

Query: 421  KTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVN 480
              QIKQFSNDTSFVCSKSFKITDLNYPSISIP L+S+  +KIKRR+KNVGSPGTYVVQVN
Sbjct: 1380 IKQIKQFSNDTSFVCSKSFKITDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVN 1439

Query: 481  APPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDG-IHRVRSPI 540
            AP GVSVSVEPTSLKFTGIDEEKSFRVV+KS+VPN F  +YVFG+LEWSDG  HRVRSPI
Sbjct: 1440 APLGVSVSVEPTSLKFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPI 1499

Query: 541  VVRLG 545
            VV+LG
Sbjct: 1500 VVKLG 1504

[Close]

BLAST of HG10003281 vs. NCBI nr
Match: TYK19382.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])

HSP 1 Score: 907.5 bits (2344), Expect = 5.5e-260
Identity = 453/545 (83.12%), Postives = 492/545 (90.28%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           G+FV  AN+FG+GNGTAKGGSPKALVAAYKVCWP+L+L GECF+ADILAGFEAAI+DGVD
Sbjct: 266 GNFVPNANLFGYGNGTAKGGSPKALVAAYKVCWPKLWLFGECFDADILAGFEAAIADGVD 325

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           VLSVSLGGD  DF QDSIAIG+FHAVQNGITVVCSAGNSGP PG+VSNVAPWIITVGAST
Sbjct: 326 VLSVSLGGDPSDFGQDSIAIGSFHAVQNGITVVCSAGNSGPAPGTVSNVAPWIITVGAST 385

Query: 121 IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
            DRL+T+YVA+GDKRH KGASLS+K LP QKFYPLIN+VDAK  N S  YA LCE  SLD
Sbjct: 386 ADRLYTNYVAIGDKRHFKGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLD 445

Query: 181 PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
           P+K KGKIV+CLRGDNAR +KG+V AKAG VGMILAN +ENGD++ ADAH LPASHITYS
Sbjct: 446 PEKVKGKIVVCLRGDNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYS 505

Query: 241 DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
           DGQLVYQYINSTKIPMAY+TH RTE GVKPAP+MASFSSRGPN+IDPSILKPDITAPG N
Sbjct: 506 DGQLVYQYINSTKIPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGEN 565

Query: 301 ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
           ILAAYSED SP+G   DKRRV F+V+SGTSMSCPH+SGIVGLLKTLYPKWSPAAIRSAIM
Sbjct: 566 ILAAYSEDGSPSGLYFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIM 625

Query: 361 TTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYN 420
           TTAGTKANDLNPILST Q KAN   YGAGHVRPNKAA+PGLVYDLST+DYLNYLCA GY 
Sbjct: 626 TTAGTKANDLNPILSTKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYK 685

Query: 421 KTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVN 480
             QIKQFSNDTSFVCSKSFKI DLNYPSISIP L+S+  +KIKRR+KNVGSPGTYVVQVN
Sbjct: 686 IKQIKQFSNDTSFVCSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVN 745

Query: 481 APPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDG-IHRVRSPI 540
           AP GVSVSVEPTSLKFTGIDEEKSFRVV+KS+VPN F  +YVFG+LEWSDG  HRVRSPI
Sbjct: 746 APLGVSVSVEPTSLKFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPI 805

Query: 541 VVRLG 545
           VV+LG
Sbjct: 806 VVKLG 810

[Close]

BLAST of HG10003281 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 5.6e-183
Identity = 325/543 (59.85%), Postives = 398/543 (73.30%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           G+FV GAN+FG GNGTA GGSPKA VAAYKVCWP +    ECF+ADILA  EAAI DGVD
Sbjct: 240 GNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVD-GAECFDADILAAIEAAIEDGVD 299

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           VLS S+GGD+ D+  D IAIG+FHAV+NG+TVVCSAGNSGP  G+VSNVAPW+ITVGAS+
Sbjct: 300 VLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASS 359

Query: 121 IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
           +DR F ++V L + +  KG SLS K LPE+K Y LI+A DA   N +   ALLC++GSLD
Sbjct: 360 MDREFQAFVELKNGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLD 419

Query: 181 PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
           PKK KGKI++CLRGDNARVDKG  AA AGA GM+L NDK +G+E+++DAH LPAS I Y 
Sbjct: 420 PKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYK 479

Query: 241 DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
           DG+ ++ Y++STK P  Y+      L  KPAP MASFSSRGPNTI P ILKPDITAPGVN
Sbjct: 480 DGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVN 539

Query: 301 ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
           I+AA++E T PT    D RR  FN +SGTSMSCPHISG+VGLLKTL+P WSPAAIRSAIM
Sbjct: 540 IIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIM 599

Query: 361 TTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYN 420
           TT+ T+ N   P++  +  KAN   YG+GHV+PNKAA+PGLVYDL+T DYL++LCA GYN
Sbjct: 600 TTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYN 659

Query: 421 KTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVN 480
            T ++ F+ D  + C +   + D NYPSI++P L     + + R++KNVG P TY  +  
Sbjct: 660 NTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGS--ITVTRKLKNVGPPATYNARFR 719

Query: 481 APPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDGIHRVRSPIV 540
            P GV VSVEP  L F    E K F++ ++       P  YVFG L W+D  H VRSPIV
Sbjct: 720 EPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVT--PSGYVFGELTWTDSHHYVRSPIV 776

Query: 541 VRL 544
           V+L
Sbjct: 780 VQL 776

[Close]

BLAST of HG10003281 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 1.5e-175
Identity = 317/543 (58.38%), Postives = 388/543 (71.45%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           G FV G +IFG GNGTAKGGSP+A VAAYKVCWP +    EC++AD+LA F+AAI DG D
Sbjct: 233 GDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPV-KGNECYDADVLAAFDAAIHDGAD 292

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           V+SVSLGG+   F  DS+AIG+FHA +  I VVCSAGNSGP   +VSNVAPW ITVGAST
Sbjct: 293 VISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGAST 352

Query: 121 IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
           +DR F S + LG+ +H KG SLS   LP  KFYP++ +V+AKA N S   A LC+ GSLD
Sbjct: 353 MDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLD 412

Query: 181 PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
           P K KGKI++CLRG N RV+KG   A  G +GM+L N    G++LLAD H LPA+ +T  
Sbjct: 413 PIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSK 472

Query: 241 DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
           D   V +YI+ TK P+A++T  RT+LG+KPAPVMASFSS+GP+ + P ILKPDITAPGV+
Sbjct: 473 DSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVS 532

Query: 301 ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
           ++AAY+   SPT    D RR+ FN  SGTSMSCPHISGI GLLKT YP WSPAAIRSAIM
Sbjct: 533 VIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIM 592

Query: 361 TTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYN 420
           TTA    +   PI +   +KA    +GAGHV+PN A NPGLVYDL  KDYLN+LC+ GYN
Sbjct: 593 TTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYN 652

Query: 421 KTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVN 480
            +QI  FS +     S    + +LNYPSI++P L S  V  + R VKNVG P  Y V+VN
Sbjct: 653 ASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVT-VSRTVKNVGRPSMYTVKVN 712

Query: 481 APPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDGIHRVRSPIV 540
            P GV V+V+PTSL FT + E+K+F+V++  S  N   + YVFG L WSD  HRVRSPIV
Sbjct: 713 NPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGN-VAKGYVFGELVWSDKKHRVRSPIV 772

Query: 541 VRL 544
           V+L
Sbjct: 773 VKL 772

[Close]

BLAST of HG10003281 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 7.2e-138
Identity = 274/553 (49.55%), Postives = 362/553 (65.46%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           G  VEGA++ G+ +GTA+G +P+A VA YKVCW     +G CF +DILA  + AI+D V+
Sbjct: 222 GSVVEGASLLGYASGTARGMAPRARVAVYKVCW-----LGGCFSSDILAAIDKAIADNVN 281

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           VLS+SLGG   D+ +D +AIGAF A++ GI V CSAGN+GP+  S+SNVAPWI TVGA T
Sbjct: 282 VLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGT 341

Query: 121 IDRLFTSYVALGDKRH------IKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLC 180
           +DR F +   LG+ ++       KG +L DK+LP   F    NA +A   N       LC
Sbjct: 342 LDRDFPALAILGNGKNFTGVSLFKGEALPDKLLP---FIYAGNASNATNGN-------LC 401

Query: 181 EEGSLDPKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPA 240
             G+L P+K KGKIV+C RG NARV KG V   AG VGMILAN   NG+EL+ADAH LPA
Sbjct: 402 MTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPA 461

Query: 241 SHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDI 300
           + +    G ++  Y+ +   P A ++ + T +GVKP+PV+A+FSSRGPN+I P+ILKPD+
Sbjct: 462 TTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDL 521

Query: 301 TAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAA 360
            APGVNILAA++    PTG + D RRV FN+ SGTSMSCPH+SG+  LLK+++P+WSPAA
Sbjct: 522 IAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAA 581

Query: 361 IRSAIMTTAGTKANDLNPILSTNQVK-ANALEYGAGHVRPNKAANPGLVYDLSTKDYLNY 420
           IRSA+MTTA     D  P+L     K +   ++GAGHV P  A NPGL+YDL+T+DYL +
Sbjct: 582 IRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGF 641

Query: 421 LCARGYNKTQIKQFSNDTSFVC--SKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGS 480
           LCA  Y   QI+  S   ++ C  SKS+ + DLNYPS ++  +      K  R V +VG 
Sbjct: 642 LCALNYTSPQIRSVSR-RNYTCDPSKSYSVADLNYPSFAV-NVDGVGAYKYTRTVTSVGG 701

Query: 481 PGTYVVQVNA-PPGVSVSVEPTSLKFTGIDEEKSFRV--VVKSSVPNDFPRKYVFGRLEW 540
            GTY V+V +   GV +SVEP  L F   +E+KS+ V   V SS P+       FG +EW
Sbjct: 702 AGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSG---SNSFGSIEW 754

Query: 541 SDGIHRVRSPIVV 542
           SDG H V SP+ +
Sbjct: 762 SDGKHVVGSPVAI 754

[Close]

BLAST of HG10003281 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 492.3 bits (1266), Expect = 7.2e-138
Identity = 285/559 (50.98%), Postives = 354/559 (63.33%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           G+FV GA +F  GNGTAKGGSP+A VAAYKVCW  L     C+ AD+LA  + AI DGVD
Sbjct: 234 GNFVPGARVFAVGNGTAKGGSPRARVAAYKVCW-SLTDPASCYGADVLAAIDQAIDDGVD 293

Query: 61  VLSVSLGGD----SQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITV 120
           V++VS G      ++    D I+IGAFHA+   I +V SAGN GPTPG+V+NVAPW+ T+
Sbjct: 294 VINVSFGVSYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTI 353

Query: 121 GASTIDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEE 180
            AST+DR F+S + + ++  I+GASL   + P Q F  LI + DAK  N +   A LC  
Sbjct: 354 AASTLDRDFSSNLTINNQL-IEGASLFVNLPPNQAF-SLILSTDAKLANATFRDAQLCRR 413

Query: 181 GSLDPKKAKGKIVICLR-GDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPAS 240
           G+LD  K  GKIV+C R G    V +G  A  AGA GMIL N  +NG  L A+ H     
Sbjct: 414 GTLDRTKVNGKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTV 473

Query: 241 HITYSDGQLVYQYINSTKI----------PMAYMTHVRTELGVKPAPVMASFSSRGPNTI 300
           +      +     + +T I              M+  RT  G KPAPVMASFSSRGPN I
Sbjct: 474 NTPPRRAKSRPHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKI 533

Query: 301 DPSILKPDITAPGVNILAAYSEDTSPTGSSLDKRR-VAFNVDSGTSMSCPHISGIVGLLK 360
            PSILKPD+TAPGVNILAAYSE  S +   +D RR   FNV  GTSMSCPH SGI GLLK
Sbjct: 534 QPSILKPDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLK 593

Query: 361 TLYPKWSPAAIRSAIMTTAGTKANDLNPILST-NQVKANALEYGAGHVRPNKAANPGLVY 420
           T +P WSPAAI+SAIMTTA T  N   PI    ++  A+A  YG+GHVRP+ A  PGLVY
Sbjct: 594 TRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVY 653

Query: 421 DLSTKDYLNYLCARGYNKTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIK 480
           DLS  DYLN+LCA GY++  I   + + +F+CS S  + DLNYPSI++P L+ + V  I 
Sbjct: 654 DLSLTDYLNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVT-IA 713

Query: 481 RRVKNVGSPGTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVF 540
           R V NVG P TY V   +P G S++V P SL FT I E K+F+V+V++S      RKY F
Sbjct: 714 RTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAAT-RRKYEF 773

Query: 541 GRLEWSDGIHRVRSPIVVR 543
           G L W+DG H VRSPI V+
Sbjct: 774 GDLRWTDGKHIVRSPITVK 787

[Close]

BLAST of HG10003281 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.6e-127
Identity = 256/542 (47.23%), Postives = 337/542 (62.18%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           G  V  A+  G+  GTA+G + +A VA YKVCW        CF +DILA  + AI DGVD
Sbjct: 218 GSAVRNASFLGYAAGTARGMATRARVATYKVCWST-----GCFGSDILAAMDRAILDGVD 277

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           VLS+SLGG S  + +D+IAIGAF A++ G+ V CSAGNSGPT  SV+NVAPW++TVGA T
Sbjct: 278 VLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGT 337

Query: 121 IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
           +DR F ++  LG+ + + G SL   +    K  PL   V  K N+ S +   LC  GSLD
Sbjct: 338 LDRDFPAFANLGNGKRLTGVSLYSGVGMGTK--PL-ELVYNKGNSSSSN---LCLPGSLD 397

Query: 181 PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
               +GKIV+C RG NARV+KG V   AG +GMI+AN   +G+EL+AD+H LPA  +   
Sbjct: 398 SSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKK 457

Query: 241 DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
            G L+ +Y+ S   P A +    T L VKP+PV+A+FSSRGPNT+ P ILKPD+  PGVN
Sbjct: 458 TGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVN 517

Query: 301 ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
           ILA +S+   PTG   D RR  FN+ SGTSMSCPHISG+ GLLK  +P+WSP+AI+SA+M
Sbjct: 518 ILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALM 577

Query: 361 TTAGTKANDLNPIL-STNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGY 420
           TTA    N   P+  + +   +N   +G+GHV P KA +PGLVYD+ST++Y+ +LC+  Y
Sbjct: 578 TTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDY 637

Query: 421 NKTQIKQFSNDTSFVCSKSFKIT-DLNYPSISIPELKSEAVVKIKRRVKNVGSPGT-YVV 480
               I       S  CSK F     LNYPS S+     + VV+  R V NVG+  + Y V
Sbjct: 638 TVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV-LFGGKRVVRYTREVTNVGAASSVYKV 697

Query: 481 QVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDGIHRVRS 540
            VN  P V +SV+P+ L F  + E+K + V   S        K  FG + WS+  H VRS
Sbjct: 698 TVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRS 747

[Close]

BLAST of HG10003281 vs. ExPASy TrEMBL
Match: A0A0A0L2R9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045120 PE=3 SV=1)

HSP 1 Score: 929.5 bits (2401), Expect = 6.5e-267
Identity = 460/545 (84.40%), Postives = 496/545 (91.01%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           GHFV  AN+FG+GNGTAKGGSPKALVAAYKVCWPQ+   GECF+ADILAGFEAAI DGVD
Sbjct: 216 GHFVPNANLFGYGNGTAKGGSPKALVAAYKVCWPQVLFFGECFDADILAGFEAAIGDGVD 275

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           VLSVSLGG   DFA+DSI+IG+FHAVQNGI VVCSAGNSGPTPGSVSNVAPWIITVGAST
Sbjct: 276 VLSVSLGGSPSDFAKDSISIGSFHAVQNGIVVVCSAGNSGPTPGSVSNVAPWIITVGAST 335

Query: 121 IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
            DRL+TSYVA+GDKRH KGAS+SDK LP QKFYPLI+++DAKA NV+++ ALLCEEGSLD
Sbjct: 336 TDRLYTSYVAIGDKRHFKGASVSDKKLPVQKFYPLISSLDAKAKNVTDNDALLCEEGSLD 395

Query: 181 PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
           PKK  GKI+ICLRGDNARV KG+VAAKAGAVGMILAN +ENGDE+LADAH LPASHITYS
Sbjct: 396 PKKVNGKIIICLRGDNARVAKGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYS 455

Query: 241 DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
           DGQLVYQYINSTKIPMAYMTHVRTE G+KPAPVMASFSSRGPNT+DPSILKPDITAPG N
Sbjct: 456 DGQLVYQYINSTKIPMAYMTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGEN 515

Query: 301 ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
           ILAAYS D SPTG+  DKRRV FNV+SGTSMSCPH+SGIVGLLKTLYPKWSPAAIRSAIM
Sbjct: 516 ILAAYSRDASPTGTDFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIM 575

Query: 361 TTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYN 420
           TTAGTKANDL PILSTNQ KANA  YGAGHV PN+AA+PGLVYDLSTKDYLNYLCA+GYN
Sbjct: 576 TTAGTKANDLTPILSTNQEKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYN 635

Query: 421 KTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVN 480
             QIKQFSNDTSFVCSKSFK+TDLNYPSISIP L+ +  VKIKR++KNVGSPGTYVVQV 
Sbjct: 636 TAQIKQFSNDTSFVCSKSFKLTDLNYPSISIPILEYDVAVKIKRKLKNVGSPGTYVVQVK 695

Query: 481 APPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDGIHRVRSPIV 540
            P GVSVSVEPTSLKFTGIDEEKSFRVV+KS  PN    KY+FG+LEWSDG HRVRSPIV
Sbjct: 696 EPLGVSVSVEPTSLKFTGIDEEKSFRVVLKSYEPNGSGPKYLFGKLEWSDGKHRVRSPIV 755

Query: 541 VRLGG 546
           VRLGG
Sbjct: 756 VRLGG 760

[Close]

BLAST of HG10003281 vs. ExPASy TrEMBL
Match: A0A1S4DX85 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491136 PE=3 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 1.1e-261
Identity = 455/545 (83.49%), Postives = 493/545 (90.46%), Query Frame = 0


Query: 1    GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
            G+FV  AN+FG+GNGTAKGGSPKALVAAYKVCWP+L+L GECF+ADILAGFEAAI+DGVD
Sbjct: 960  GNFVPNANLFGYGNGTAKGGSPKALVAAYKVCWPKLWLFGECFDADILAGFEAAIADGVD 1019

Query: 61   VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
            VLSVSLGGD  DF QDSIAIG+FHAVQNGITVVCSAGNSGP PG+VSNVAPWIITVGAST
Sbjct: 1020 VLSVSLGGDPSDFGQDSIAIGSFHAVQNGITVVCSAGNSGPAPGTVSNVAPWIITVGAST 1079

Query: 121  IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
             DRL+T+YVA+GDKRH KGASLS+K LP QKFYPLIN+VDAK  N S  YA LCE  SLD
Sbjct: 1080 ADRLYTNYVAIGDKRHFKGASLSEKGLPVQKFYPLINSVDAKVQNFSNKYAQLCEIESLD 1139

Query: 181  PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
            P+K KGKIV+CLRGDNAR +KG+V AKAG VGMILAN +ENGD++ ADAH LPASHITYS
Sbjct: 1140 PEKVKGKIVVCLRGDNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYS 1199

Query: 241  DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
            DGQLVYQYINSTKIPMAY+TH RTE GVKPAP+MASFSSRGPN+IDPSILKPDITAPG N
Sbjct: 1200 DGQLVYQYINSTKIPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGEN 1259

Query: 301  ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
            ILAAYSED SP+G   DKRRV FNV+SGTSMSCPH+SGIVGLLKTLYPKWSPAAIRSAIM
Sbjct: 1260 ILAAYSEDGSPSGLYFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIM 1319

Query: 361  TTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYN 420
            TTAGTKANDLNPILST Q KAN   YGAGHVRPNKAA+PGLVYDLST+DYLNYLCA GY 
Sbjct: 1320 TTAGTKANDLNPILSTKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYK 1379

Query: 421  KTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVN 480
              QIKQFSNDTSFVCSKSFKITDLNYPSISIP L+S+  +KIKRR+KNVGSPGTYVVQVN
Sbjct: 1380 IKQIKQFSNDTSFVCSKSFKITDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVN 1439

Query: 481  APPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDG-IHRVRSPI 540
            AP GVSVSVEPTSLKFTGIDEEKSFRVV+KS+VPN F  +YVFG+LEWSDG  HRVRSPI
Sbjct: 1440 APLGVSVSVEPTSLKFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPI 1499

Query: 541  VVRLG 545
            VV+LG
Sbjct: 1500 VVKLG 1504

[Close]

BLAST of HG10003281 vs. ExPASy TrEMBL
Match: A0A5D3D773 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00130 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.7e-260
Identity = 453/545 (83.12%), Postives = 492/545 (90.28%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           G+FV  AN+FG+GNGTAKGGSPKALVAAYKVCWP+L+L GECF+ADILAGFEAAI+DGVD
Sbjct: 266 GNFVPNANLFGYGNGTAKGGSPKALVAAYKVCWPKLWLFGECFDADILAGFEAAIADGVD 325

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           VLSVSLGGD  DF QDSIAIG+FHAVQNGITVVCSAGNSGP PG+VSNVAPWIITVGAST
Sbjct: 326 VLSVSLGGDPSDFGQDSIAIGSFHAVQNGITVVCSAGNSGPAPGTVSNVAPWIITVGAST 385

Query: 121 IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
            DRL+T+YVA+GDKRH KGASLS+K LP QKFYPLIN+VDAK  N S  YA LCE  SLD
Sbjct: 386 ADRLYTNYVAIGDKRHFKGASLSEKGLPIQKFYPLINSVDAKVQNFSNKYAQLCEIESLD 445

Query: 181 PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
           P+K KGKIV+CLRGDNAR +KG+V AKAG VGMILAN +ENGD++ ADAH LPASHITYS
Sbjct: 446 PEKVKGKIVVCLRGDNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYS 505

Query: 241 DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
           DGQLVYQYINSTKIPMAY+TH RTE GVKPAP+MASFSSRGPN+IDPSILKPDITAPG N
Sbjct: 506 DGQLVYQYINSTKIPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGEN 565

Query: 301 ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
           ILAAYSED SP+G   DKRRV F+V+SGTSMSCPH+SGIVGLLKTLYPKWSPAAIRSAIM
Sbjct: 566 ILAAYSEDGSPSGLYFDKRRVPFSVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIM 625

Query: 361 TTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYN 420
           TTAGTKANDLNPILST Q KAN   YGAGHVRPNKAA+PGLVYDLST+DYLNYLCA GY 
Sbjct: 626 TTAGTKANDLNPILSTKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYK 685

Query: 421 KTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVN 480
             QIKQFSNDTSFVCSKSFKI DLNYPSISIP L+S+  +KIKRR+KNVGSPGTYVVQVN
Sbjct: 686 IKQIKQFSNDTSFVCSKSFKIIDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVN 745

Query: 481 APPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDG-IHRVRSPI 540
           AP GVSVSVEPTSLKFTGIDEEKSFRVV+KS+VPN F  +YVFG+LEWSDG  HRVRSPI
Sbjct: 746 APLGVSVSVEPTSLKFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPI 805

Query: 541 VVRLG 545
           VV+LG
Sbjct: 806 VVKLG 810

[Close]

BLAST of HG10003281 vs. ExPASy TrEMBL
Match: A0A6J1CQB4 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111013335 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 4.4e-239
Identity = 420/544 (77.21%), Postives = 474/544 (87.13%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           G+FV GA++FG GNGTAKGGSP+A VAAY+VCWP + L G CF ADILAGFEAAI DGVD
Sbjct: 229 GNFVSGASVFGNGNGTAKGGSPRARVAAYRVCWPAV-LTGGCFMADILAGFEAAIHDGVD 288

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           VLSVSLGG  ++F+ D +AIGAFHAVQ+GITVVCSAGNSGP  G+VSNVAPW+ITVGAST
Sbjct: 289 VLSVSLGGSPEEFSDDGLAIGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGAST 348

Query: 121 IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
            DRLF SYV LG+++HIKGASLSDKILP QKFYPLI+  DAKA+NVS +YA LCEEGSLD
Sbjct: 349 ADRLFASYVGLGNRKHIKGASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLD 408

Query: 181 PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
           PKK +GKIV+CLRGDNARVDKG+VAA+AGAVGMILAN ++NGDELLADAH LPASH++Y+
Sbjct: 409 PKKVEGKIVVCLRGDNARVDKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYA 468

Query: 241 DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
           DG+L++QYI STKIPMAYMTHV+TELGVKPAP MASFSSRGPNTI+ SILKPDI APGV+
Sbjct: 469 DGELIFQYIKSTKIPMAYMTHVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVS 528

Query: 301 ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
           I+AAYSE+ SP+GSS DKRR  FN +SGTSMSCPH+SGIVGLLKT YPKWSPAAI+SA+M
Sbjct: 529 IIAAYSEEASPSGSSFDKRRSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALM 588

Query: 361 TTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYN 420
           TTA TKANDL+PIL+T Q+KAN L YGAGHVRPNKA NPGLVYDL+ KDYLN+LCARGYN
Sbjct: 589 TTAATKANDLHPILNTTQLKANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYN 648

Query: 421 KTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVN 480
           +TQIK+FS+   F CS SFK+TD NYPSISIP LK    VK KRRVKNVGSPGTYV QV 
Sbjct: 649 QTQIKKFSS-APFACSHSFKLTDFNYPSISIPNLKF-GPVKAKRRVKNVGSPGTYVAQVK 708

Query: 481 APPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDGIHRVRSPIV 540
           APPGV+VSVEP  LKFTGI EE+SFRVVV+  V N+  R YVFG L WSDG HRVRSPI 
Sbjct: 709 APPGVAVSVEPNMLKFTGIGEEQSFRVVVR-RVENEERRGYVFGWLAWSDGNHRVRSPIA 768

Query: 541 VRLG 545
           V LG
Sbjct: 769 VNLG 768

[Close]

BLAST of HG10003281 vs. ExPASy TrEMBL
Match: A0A6J1L3R2 (subtilisin-like protease SBT5.4 OS=Cucurbita maxima OX=3661 GN=LOC111500241 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 1.1e-237
Identity = 420/544 (77.21%), Postives = 469/544 (86.21%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           GHFV  ANIFG GNGTAKGGSPKALVAAYKVCWP++  +G CF++DILAGFEAAISDGVD
Sbjct: 229 GHFVSRANIFGNGNGTAKGGSPKALVAAYKVCWPRV-NIGGCFDSDILAGFEAAISDGVD 288

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           VLS+SLGG   +F  D IAI +FHAV+NGITVVCSAGNSGP+PG+V+NVAPW+ITVGAST
Sbjct: 289 VLSISLGGSPLEFHDDPIAIASFHAVKNGITVVCSAGNSGPSPGTVANVAPWLITVGAST 348

Query: 121 IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
           IDRLFT+Y+ALGDKRHIKG S+S+KILP QKFYPLI A+DAK NNVS   A+LC EGSLD
Sbjct: 349 IDRLFTTYLALGDKRHIKGESISNKILPVQKFYPLIRALDAKNNNVSNIEAILCLEGSLD 408

Query: 181 PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
           PKK +GKIV+CL GD A VDKG+VAA+AGAVGMIL NDKE+GD L  +AH LP SHI Y 
Sbjct: 409 PKKVEGKIVVCLSGDIANVDKGYVAAQAGAVGMILTNDKEDGDRLEVEAHLLPTSHINYI 468

Query: 241 DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
           DG+LVYQ+INSTK P+AYMTHVRTELGVKPAP+MA FSSRGPN I+PSILKPDITAPGVN
Sbjct: 469 DGELVYQFINSTKTPIAYMTHVRTELGVKPAPIMARFSSRGPNIIEPSILKPDITAPGVN 528

Query: 301 ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
           ILAA++E+TSPTGSS DKRRV FN+ SGTSMSCPHISGIVGLLKTLYPKWSPA ++S IM
Sbjct: 529 ILAAFTEETSPTGSSFDKRRVPFNILSGTSMSCPHISGIVGLLKTLYPKWSPAVMKSTIM 588

Query: 361 TTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYN 420
           TTA  KANDLN ILS+ + KAN+  YGAGHV PNKAANPGLVYDLST+DYLN+LCARGYN
Sbjct: 589 TTARIKANDLNSILSSGKEKANSFAYGAGHVNPNKAANPGLVYDLSTEDYLNFLCARGYN 648

Query: 421 KTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVN 480
           KTQ+K FSNDTSFVCSK FK+ DLNYPSI+I  L SE VVKIKRRVKNVGSP TYV  V 
Sbjct: 649 KTQMKLFSNDTSFVCSKKFKVADLNYPSITIDNLASE-VVKIKRRVKNVGSPATYVANVK 708

Query: 481 APPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDGIHRVRSPIV 540
           APP VSVSVEP +LKFT   EEKSF VV+K  VPND+ R  VFGRL WS+G H VRSPI+
Sbjct: 709 APPRVSVSVEPNTLKFTRTGEEKSFTVVLK-RVPNDYHRGSVFGRLAWSNGKHHVRSPIL 768

Query: 541 VRLG 545
           V LG
Sbjct: 769 VVLG 769

[Close]

BLAST of HG10003281 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 642.1 bits (1655), Expect = 4.0e-184
Identity = 325/543 (59.85%), Postives = 398/543 (73.30%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           G+FV GAN+FG GNGTA GGSPKA VAAYKVCWP +    ECF+ADILA  EAAI DGVD
Sbjct: 240 GNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVD-GAECFDADILAAIEAAIEDGVD 299

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           VLS S+GGD+ D+  D IAIG+FHAV+NG+TVVCSAGNSGP  G+VSNVAPW+ITVGAS+
Sbjct: 300 VLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASS 359

Query: 121 IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
           +DR F ++V L + +  KG SLS K LPE+K Y LI+A DA   N +   ALLC++GSLD
Sbjct: 360 MDREFQAFVELKNGQSFKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLD 419

Query: 181 PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
           PKK KGKI++CLRGDNARVDKG  AA AGA GM+L NDK +G+E+++DAH LPAS I Y 
Sbjct: 420 PKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYK 479

Query: 241 DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
           DG+ ++ Y++STK P  Y+      L  KPAP MASFSSRGPNTI P ILKPDITAPGVN
Sbjct: 480 DGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVN 539

Query: 301 ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
           I+AA++E T PT    D RR  FN +SGTSMSCPHISG+VGLLKTL+P WSPAAIRSAIM
Sbjct: 540 IIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIM 599

Query: 361 TTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYN 420
           TT+ T+ N   P++  +  KAN   YG+GHV+PNKAA+PGLVYDL+T DYL++LCA GYN
Sbjct: 600 TTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYN 659

Query: 421 KTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVN 480
            T ++ F+ D  + C +   + D NYPSI++P L     + + R++KNVG P TY  +  
Sbjct: 660 NTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGS--ITVTRKLKNVGPPATYNARFR 719

Query: 481 APPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDGIHRVRSPIV 540
            P GV VSVEP  L F    E K F++ ++       P  YVFG L W+D  H VRSPIV
Sbjct: 720 EPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVT--PSGYVFGELTWTDSHHYVRSPIV 776

Query: 541 VRL 544
           V+L
Sbjct: 780 VQL 776

[Close]

BLAST of HG10003281 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 617.5 bits (1591), Expect = 1.1e-176
Identity = 317/543 (58.38%), Postives = 388/543 (71.45%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           G FV G +IFG GNGTAKGGSP+A VAAYKVCWP +    EC++AD+LA F+AAI DG D
Sbjct: 233 GDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPV-KGNECYDADVLAAFDAAIHDGAD 292

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           V+SVSLGG+   F  DS+AIG+FHA +  I VVCSAGNSGP   +VSNVAPW ITVGAST
Sbjct: 293 VISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGAST 352

Query: 121 IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
           +DR F S + LG+ +H KG SLS   LP  KFYP++ +V+AKA N S   A LC+ GSLD
Sbjct: 353 MDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLD 412

Query: 181 PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
           P K KGKI++CLRG N RV+KG   A  G +GM+L N    G++LLAD H LPA+ +T  
Sbjct: 413 PIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSK 472

Query: 241 DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
           D   V +YI+ TK P+A++T  RT+LG+KPAPVMASFSS+GP+ + P ILKPDITAPGV+
Sbjct: 473 DSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVS 532

Query: 301 ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
           ++AAY+   SPT    D RR+ FN  SGTSMSCPHISGI GLLKT YP WSPAAIRSAIM
Sbjct: 533 VIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIM 592

Query: 361 TTAGTKANDLNPILSTNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGYN 420
           TTA    +   PI +   +KA    +GAGHV+PN A NPGLVYDL  KDYLN+LC+ GYN
Sbjct: 593 TTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYN 652

Query: 421 KTQIKQFSNDTSFVCSKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGSPGTYVVQVN 480
            +QI  FS +     S    + +LNYPSI++P L S  V  + R VKNVG P  Y V+VN
Sbjct: 653 ASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVT-VSRTVKNVGRPSMYTVKVN 712

Query: 481 APPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDGIHRVRSPIV 540
            P GV V+V+PTSL FT + E+K+F+V++  S  N   + YVFG L WSD  HRVRSPIV
Sbjct: 713 NPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGN-VAKGYVFGELVWSDKKHRVRSPIV 772

Query: 541 VRL 544
           V+L
Sbjct: 773 VKL 772

[Close]

BLAST of HG10003281 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 492.3 bits (1266), Expect = 5.1e-139
Identity = 274/553 (49.55%), Postives = 362/553 (65.46%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           G  VEGA++ G+ +GTA+G +P+A VA YKVCW     +G CF +DILA  + AI+D V+
Sbjct: 222 GSVVEGASLLGYASGTARGMAPRARVAVYKVCW-----LGGCFSSDILAAIDKAIADNVN 281

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           VLS+SLGG   D+ +D +AIGAF A++ GI V CSAGN+GP+  S+SNVAPWI TVGA T
Sbjct: 282 VLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGT 341

Query: 121 IDRLFTSYVALGDKRH------IKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLC 180
           +DR F +   LG+ ++       KG +L DK+LP   F    NA +A   N       LC
Sbjct: 342 LDRDFPALAILGNGKNFTGVSLFKGEALPDKLLP---FIYAGNASNATNGN-------LC 401

Query: 181 EEGSLDPKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPA 240
             G+L P+K KGKIV+C RG NARV KG V   AG VGMILAN   NG+EL+ADAH LPA
Sbjct: 402 MTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPA 461

Query: 241 SHITYSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDI 300
           + +    G ++  Y+ +   P A ++ + T +GVKP+PV+A+FSSRGPN+I P+ILKPD+
Sbjct: 462 TTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDL 521

Query: 301 TAPGVNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAA 360
            APGVNILAA++    PTG + D RRV FN+ SGTSMSCPH+SG+  LLK+++P+WSPAA
Sbjct: 522 IAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAA 581

Query: 361 IRSAIMTTAGTKANDLNPILSTNQVK-ANALEYGAGHVRPNKAANPGLVYDLSTKDYLNY 420
           IRSA+MTTA     D  P+L     K +   ++GAGHV P  A NPGL+YDL+T+DYL +
Sbjct: 582 IRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGF 641

Query: 421 LCARGYNKTQIKQFSNDTSFVC--SKSFKITDLNYPSISIPELKSEAVVKIKRRVKNVGS 480
           LCA  Y   QI+  S   ++ C  SKS+ + DLNYPS ++  +      K  R V +VG 
Sbjct: 642 LCALNYTSPQIRSVSR-RNYTCDPSKSYSVADLNYPSFAV-NVDGVGAYKYTRTVTSVGG 701

Query: 481 PGTYVVQVNA-PPGVSVSVEPTSLKFTGIDEEKSFRV--VVKSSVPNDFPRKYVFGRLEW 540
            GTY V+V +   GV +SVEP  L F   +E+KS+ V   V SS P+       FG +EW
Sbjct: 702 AGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSG---SNSFGSIEW 754

Query: 541 SDGIHRVRSPIVV 542
           SDG H V SP+ +
Sbjct: 762 SDGKHVVGSPVAI 754

[Close]

BLAST of HG10003281 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 457.2 bits (1175), Expect = 1.8e-128
Identity = 256/542 (47.23%), Postives = 337/542 (62.18%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           G  V  A+  G+  GTA+G + +A VA YKVCW        CF +DILA  + AI DGVD
Sbjct: 218 GSAVRNASFLGYAAGTARGMATRARVATYKVCWST-----GCFGSDILAAMDRAILDGVD 277

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           VLS+SLGG S  + +D+IAIGAF A++ G+ V CSAGNSGPT  SV+NVAPW++TVGA T
Sbjct: 278 VLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGT 337

Query: 121 IDRLFTSYVALGDKRHIKGASLSDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGSLD 180
           +DR F ++  LG+ + + G SL   +    K  PL   V  K N+ S +   LC  GSLD
Sbjct: 338 LDRDFPAFANLGNGKRLTGVSLYSGVGMGTK--PL-ELVYNKGNSSSSN---LCLPGSLD 397

Query: 181 PKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHITYS 240
               +GKIV+C RG NARV+KG V   AG +GMI+AN   +G+EL+AD+H LPA  +   
Sbjct: 398 SSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKK 457

Query: 241 DGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVN 300
            G L+ +Y+ S   P A +    T L VKP+PV+A+FSSRGPNT+ P ILKPD+  PGVN
Sbjct: 458 TGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVN 517

Query: 301 ILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIM 360
           ILA +S+   PTG   D RR  FN+ SGTSMSCPHISG+ GLLK  +P+WSP+AI+SA+M
Sbjct: 518 ILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALM 577

Query: 361 TTAGTKANDLNPIL-STNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCARGY 420
           TTA    N   P+  + +   +N   +G+GHV P KA +PGLVYD+ST++Y+ +LC+  Y
Sbjct: 578 TTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDY 637

Query: 421 NKTQIKQFSNDTSFVCSKSFKIT-DLNYPSISIPELKSEAVVKIKRRVKNVGSPGT-YVV 480
               I       S  CSK F     LNYPS S+     + VV+  R V NVG+  + Y V
Sbjct: 638 TVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV-LFGGKRVVRYTREVTNVGAASSVYKV 697

Query: 481 QVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDGIHRVRS 540
            VN  P V +SV+P+ L F  + E+K + V   S        K  FG + WS+  H VRS
Sbjct: 698 TVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRS 747

[Close]

BLAST of HG10003281 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 451.4 bits (1160), Expect = 1.0e-126
Identity = 246/549 (44.81%), Postives = 349/549 (63.57%), Query Frame = 0


Query: 1   GHFVEGANIFGFGNGTAKGGSPKALVAAYKVCWPQLFLVGECFEADILAGFEAAISDGVD 60
           G  V+GAN+FGF  GTA+G + KA VAAYKVCW     VG CF +DIL+  + A++DGV 
Sbjct: 237 GSPVKGANLFGFAYGTARGMAQKARVAAYKVCW-----VGGCFSSDILSAVDQAVADGVQ 296

Query: 61  VLSVSLGGDSQDFAQDSIAIGAFHAVQNGITVVCSAGNSGPTPGSVSNVAPWIITVGAST 120
           VLS+SLGG    +++DS++I  F A++ G+ V CSAGN GP P S++NV+PWI TVGAST
Sbjct: 297 VLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGAST 356

Query: 121 IDRLFTSYVALGDKRHIKGASL--SDKILPEQKFYPLINAVDAKANNVSEDYALLCEEGS 180
           +DR F + V +G  R  KG SL     +LP+ K YPL   V    N  S D    C +G+
Sbjct: 357 MDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPL---VYLGRNASSPDPTSFCLDGA 416

Query: 181 LDPKKAKGKIVICLRGDNARVDKGFVAAKAGAVGMILANDKENGDELLADAHFLPASHIT 240
           LD +   GKIVIC RG   RV KG V  +AG +GM+L N   NG+EL+AD+H LPA  + 
Sbjct: 417 LDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVG 476

Query: 241 YSDGQLVYQYINSTKIPMAYMTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPG 300
             +G+L+ QY  ++K   A +  + T +G+KP+PV+A+FSSRGPN +   ILKPD+ APG
Sbjct: 477 EKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPG 536

Query: 301 VNILAAYSEDTSPTGSSLDKRRVAFNVDSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSA 360
           VNILAA++ D +P+  S D RRV FN+ SGTSMSCPH+SG+  L+K+ +P WSPAAI+SA
Sbjct: 537 VNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSA 596

Query: 361 IMTTAGTKANDLNPIL-STNQVKANALEYGAGHVRPNKAANPGLVYDLSTKDYLNYLCAR 420
           +MTTA    N   P+  ++    ++  ++GAGH+ P +A +PGLVYD+  ++Y  +LC +
Sbjct: 597 LMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQ 656

Query: 421 GYNKTQIKQFSNDTSFVCSKSFKIT--DLNYPSIS--IPELKSEAVVKIKRRVKNVGSP- 480
             + +Q+K F+  ++  C  +      +LNYP+IS   PE      + ++R V NVG   
Sbjct: 657 DLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHI 716

Query: 481 GTYVVQVNAPPGVSVSVEPTSLKFTGIDEEKSFRVVVKSSVPNDFPRKYVFGRLEWSDGI 540
            +Y V V+   G SV+V+P +L FT   ++ S+ V  ++      P    FG L W    
Sbjct: 717 SSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKRPE---FGGLVWKSTT 774

Query: 541 HRVRSPIVV 542
           H+VRSP+++
Sbjct: 777 HKVRSPVII 774

The following BLAST results are available for this feature:

BLAST of HG10003281 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904154.1	2.3e-290	92.29	subtilisin-like protease SBT5.4 [Benincasa hispida]	[more]
KAE8650095.1	1.3e-266	84.40	hypothetical protein Csa_011146 [Cucumis sativus]	[more]
XP_011650462.2	1.3e-266	84.40	subtilisin-like protease SBT5.4 [Cucumis sativus]	[more]
XP_016900594.1	2.2e-261	83.49	PREDICTED: subtilisin-like protease SBT5.3, partial [Cucumis melo]	[more]
TYK19382.1	5.5e-260	83.12	subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa]	[more]

BLAST of HG10003281 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JXC5	5.6e-183	59.85	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]
Q9ZSP5	1.5e-175	58.38	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
O65351	7.2e-138	49.55	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S...	[more]
I1N462	7.2e-138	50.98	Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P...	[more]
Q9ZUF6	2.6e-127	47.23	Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S...	[more]

BLAST of HG10003281 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2R9	6.5e-267	84.40	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045120 PE=3 SV=1	[more]
A0A1S4DX85	1.1e-261	83.49	subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491136 PE=3 SV=...	[more]
A0A5D3D773	2.7e-260	83.12	Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A6J1CQB4	4.4e-239	77.21	subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111013335 P...	[more]
A0A6J1L3R2	1.1e-237	77.21	subtilisin-like protease SBT5.4 OS=Cucurbita maxima OX=3661 GN=LOC111500241 PE=3...	[more]

BLAST of HG10003281 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59810.1	4.0e-184	59.85	Subtilase family protein	[more]
AT2G04160.1	1.1e-176	58.38	Subtilisin-like serine endopeptidase family protein	[more]
AT5G67360.1	5.1e-139	49.55	Subtilase family protein	[more]
AT2G05920.1	1.8e-128	47.23	Subtilase family protein	[more]
AT5G51750.1	1.0e-126	44.81	subtilase 1.3	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003137	PA domain	PFAM	PF02225	PA	coord: 174..243 e-value: 1.6E-11 score: 44.1
None	No IPR available	GENE3D	3.50.30.30		coord: 122..266 e-value: 1.0E-146 score: 491.4
None	No IPR available	GENE3D	2.60.40.2310		coord: 414..543 e-value: 9.3E-38 score: 130.9
None	No IPR available	PANTHER	PTHR10795:SF662	SUBTILISIN-LIKE PROTEASE SBT5.4	coord: 1..544
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 1..397 score: 22.842737
None	No IPR available	CDD	cd02120	PA_subtilisin_like	coord: 128..257 e-value: 1.84101E-38 score: 135.234
None	No IPR available	SUPERFAMILY	52025	PA domain	coord: 167..257
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 443..541 e-value: 1.6E-28 score: 98.8
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 1..410 e-value: 1.0E-146 score: 491.4
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 12..401
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 17..387 e-value: 2.3E-34 score: 119.6
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 1..544
IPR023828	Peptidase S8, subtilisin, Ser-active site	PROSITE	PS00138	SUBTILASE_SER	coord: 328..338

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10003281.1	HG10003281.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity