HG10003277 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10003277 vs. NCBI nr
Match: XP_008449184.1 (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo])

HSP 1 Score: 1938.3 bits (5020), Expect = 0.0e+00
Identity = 941/1360 (69.19%), Postives = 1104/1360 (81.18%), Query Frame = 0

Query: 14   GVWPESKSFSDAGYGPVPSRWMGVCEGGSDFTCNKKLIGARYFYKGFEAANGAINATL-R 73
            GVWPESKSF+DAGYGPVPSRW G CEGG++F CN+KLIGARYF+ GF   +G IN +  +
Sbjct: 152  GVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRKLIGARYFHAGFVMGSGLINVSFNK 211

Query: 74   TARDQEGHGSHTLSTAGGNFVPGANLFGNANGTAKGGSPKARLAAYKVCWPGGL-DGGCF 133
            TARD++GHGSHTLSTAGGNFVPGA++FG  NGTAKGGSPKAR+AAY+VCWP  L  GGC+
Sbjct: 212  TARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAKGGSPKARVAAYRVCWPPALFGGGCY 271

Query: 134  DADVLAAFETAISDGVDVLSVSLGSPARDFASDPLAIGAFHAVQHGIVVVCSGGNDGPSP 193
            DAD+LA FE AISDGVDVLSVSLGS A++F+ D ++IGAFHAV+ GIVVVCS GN GP P
Sbjct: 272  DADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSMSIGAFHAVEQGIVVVCSAGNSGPGP 331

Query: 194  GTITNVSPWIFTVAASTIDRDFINYVTLGNKKHFKGASLSSGGLPGHKFYPLMNGVQVKA 253
             T++NVSPW+FTV ASTIDRDF +Y TLGNKK +KG+SLSS  L G KFYPL+N V  + 
Sbjct: 332  KTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYKGSSLSSSSLAGGKFYPLINAVDARN 391

Query: 254  ANATDNLALLCEDGSLDPTKAKGKIILCLRGDNARMDKGLEVRRAGGVGLILVNGKEDGT 313
             N+TD+ A  CE GSLDP K KGKI++CLRG  AR++KG  V +AGGVG+ILVN K DG+
Sbjct: 392  PNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTARVEKGYVVLQAGGVGMILVNDKTDGS 451

Query: 314  SITADPHLLPASHLNYADGLAIFQYVNSTKAPMALITHVTTELGLKPSPMMGDFSSRGPN 373
             + +D H+LPA+ L Y DGLA+ QY+NST  P+ALIT V T+LG+KPSP+M  FSSRGPN
Sbjct: 452  GLISDSHILPATSLTYTDGLAVAQYINSTTTPVALITPVETQLGVKPSPVMAGFSSRGPN 511

Query: 374  PITDSMIKPDITAPGVSILASVSESAPATQFPFDTRRVPFNFETGTSMACPHISGVVGLL 433
            PITD+M+KPDIT PGV+ILASV+    AT+FPFDTRRVPFN E+GTSM+CPHI+GV GLL
Sbjct: 512  PITDAMLKPDITGPGVNILASVTTDVTATKFPFDTRRVPFNVESGTSMSCPHIAGVSGLL 571

Query: 434  KTLYPKWSPAAIKSAIMTTAKTRDNSHKSILDYNKVKATPFSYGAGHVHPNNAMNPGLVY 493
            KTLYP WSPAAIKSAIMTTAKTRDNS  ++ D  K KATPF YGAGHV+PNNAM+PGLVY
Sbjct: 572  KTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDTVKAKATPFDYGAGHVNPNNAMDPGLVY 631

Query: 494  DTTMEDYMNFLCAQGYNYTALKKFYNKPFVCPKSFATTDLNYPSISVPKLTAGATVTINR 553
            DTT++DY+NFLCA+GYN   L  F++KPFVC   F  TDLNYPSIS+P+L +GA VT+NR
Sbjct: 632  DTTIDDYLNFLCARGYNAQTLMSFHSKPFVCATPFTLTDLNYPSISIPELKSGAPVTVNR 691

Query: 554  RVKNVGTPGTYVASVKGSSKVSVSVEPSTLQFNSVGEEKAFKVVFHYKGQGQGQGHVFGT 613
            RVKNVGTPGTYVA VK SSK+SV+VEPSTLQFNSVGEEKAFKV+F YKG GQG+ +VFGT
Sbjct: 692  RVKNVGTPGTYVARVKASSKISVTVEPSTLQFNSVGEEKAFKVLFEYKGTGQGKSYVFGT 751

Query: 614  LI------------------------------------------------------CKLA 673
            LI                                                       KLA
Sbjct: 752  LIWSDNGKHNVRSPIVVKLGSYIVYLGSHFFGSNPSIHDVQLATESQYELLESVVGSKLA 811

Query: 674  AKESIIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTTRSWNFLGVENDE 733
            AKESI YSYNRYINGFAA+LDE +A AL  +P+VVSIFEN++R+LHTTRSW+FLG+E+DE
Sbjct: 812  AKESIFYSYNRYINGFAAILDENQAIALARNPNVVSIFENQKRKLHTTRSWSFLGMESDE 871

Query: 734  GIPPNSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACVGGANFTCNRK 793
            GIPPNSIW+AAR GEDTII NLDTG WPESKSFNDAGYGP+PSRW G C GGANFTCN+K
Sbjct: 872  GIPPNSIWKAARFGEDTIIGNLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGANFTCNKK 931

Query: 794  LIGARYFYQGIASAKGILNNIFNTTRDHGGHGSHTLSTAGGNFVPGANIFGYGNGTAKGG 853
            LIGARYF +G  +  G ++    T RD  GHGSHTLSTAGGNFVPGAN+FG GNGTAKGG
Sbjct: 932  LIGARYFNKGFEAENGPMSANLTTARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGG 991

Query: 854  SPKARLVAYKVCWPTSSGGCFDADILAAFEAAISDGVDVLSVSLGGKAQEFSHDPISIGA 913
            SP+ARL AYKVCWP+ +GGC+DADILAA E+AI DGVDVLS+SLG  A++F+ D +SIGA
Sbjct: 992  SPRARLAAYKVCWPSFTGGCYDADILAAVESAIHDGVDVLSISLGSSARDFASDTLSIGA 1051

Query: 914  FHAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVVLGNKKQFKSPSL 973
            FHAVQQGIVVVCSGGN GP P TV+NVSPWM TVA S +DRDFVNYV LGNK+ FK  SL
Sbjct: 1052 FHAVQQGIVVVCSGGNDGPTPGTVTNVSPWMITVAASTVDRDFVNYVALGNKRHFKGVSL 1111

Query: 974  SSGELPEGKYYPLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIVCHLGDNSRIDKG 1033
            SSG LP GK+YPL++ V+ KA NA+D LA +CE GSLDP KAKGKI++C  GD++R+DK 
Sbjct: 1112 SSGGLPRGKFYPLVDGVQVKAGNATDKLALLCEDGSLDPAKAKGKIVLCLRGDSARMDKS 1171

Query: 1034 FEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMNSTKTPVALITHV 1093
            FEV R GG+G+I+VNDK++GN++  D H LPASHLNY DG++I  Y+NSTK+P+A ITHV
Sbjct: 1172 FEVRRAGGIGLILVNDKEDGNDITADPHFLPASHLNYADGIAIFQYINSTKSPMAFITHV 1231

Query: 1094 RTELGIKPSPVIAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVTATKSRFDTRRVP 1153
            +TE+GIKPSP++A FSS+GPNPI DSMIKPDI APG SI+A+F+E  TAT    DTRRV 
Sbjct: 1232 KTEMGIKPSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSEYATATDFPLDTRRVS 1291

Query: 1154 FNVQSGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTMHPILDSTNVKAT 1213
            FN +SGTSMACPHISGVVGLLKTLYP WSPAAIKSAIMTTA TRDN+M  ILD    KAT
Sbjct: 1292 FNFESGTSMACPHISGVVGLLKTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDYNKAKAT 1351

Query: 1214 PFDYGAGQVNPNNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFSNKPFVCAESFAITD 1273
            PF YGAG V+PNNAIDPGLVYDTTI+DY+NF+CA+GYN T LK+F NKP++C +SF +TD
Sbjct: 1352 PFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKRFYNKPYLCPKSFPLTD 1411

Query: 1274 LNYPSISVPKLTIDAPMTINRRVKNVGSPGTYVANVEVPEGVVVTVEPNTLQFNNVGEEK 1318
            LNYPSISVPKLTI  P+TINRR+KNVG+PGTYVA V+V   V VTV+P+TLQFN+VGEEK
Sbjct: 1412 LNYPSISVPKLTIGVPVTINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEK 1471

BLAST of HG10003277 vs. NCBI nr
Match: TYK19386.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])

HSP 1 Score: 1907.9 bits (4941), Expect = 0.0e+00
Identity = 944/1453 (64.97%), Postives = 1105/1453 (76.05%), Query Frame = 0

Query: 14   GVWPESKSFSDAGYGPVPSRWMGVCEGGSDFTCNKKLIGARYFYKGFEAANGAINATL-R 73
            GVWPESKSF+DAGYGPVPSRW G CEGG++F CN+KLIGARYF+ GF   +G IN +  +
Sbjct: 30   GVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRKLIGARYFHAGFVMGSGLINVSFNK 89

Query: 74   TARDQEGHGSHTLSTAGGNFVPGANLFGNANGTAKGGSPKARLAAYKVCWPGGL-DGGCF 133
            TARD++GHGSHTLSTAGGNFVPGA++FG  NGTAKGGSPKAR+AAY+VCWP  L  GGC+
Sbjct: 90   TARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAKGGSPKARVAAYRVCWPPALFGGGCY 149

Query: 134  DADVLAAFETAISDGVDVLSVSLGSPARDFASDPLAIGAFHAVQHGIVVVCSGGNDGPSP 193
            DAD+LA FE AISDGVDVLSVSLGS A++F+ D ++IGAFHAV+ GIVVVCS GN GP P
Sbjct: 150  DADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSMSIGAFHAVEQGIVVVCSAGNSGPGP 209

Query: 194  GTITNVSPWIFTVAASTIDRDFINYVTLGNKKHFKGASLSSGGLPGHKFYPLMNGVQVKA 253
             T++NVSPW+FTV ASTIDRDF +Y TLGNKK +KG+SLSS  L G KFYPL+N V  + 
Sbjct: 210  KTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYKGSSLSSSSLAGGKFYPLINAVDARN 269

Query: 254  ANATDNLALLCEDGSLDPTKAKGKIILCLRGDNARMDKGLEVRRAGGVGLILVNGKEDGT 313
             N+TD+ A  CE GSLDP K KGKI++CLRG  AR++KG  V +AGGVG+ILVN K DG+
Sbjct: 270  PNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTARVEKGYVVLQAGGVGMILVNDKTDGS 329

Query: 314  SITADPHLLPASHLNYADGLAIFQYVNSTKAPMALITHVTTELGLKPSPMMGDFSSRGPN 373
             + +D H+LPA+ L Y DGLA+ QY+NST  P+ALIT V T+LG+KPSP+M  FSSRGPN
Sbjct: 330  GLISDSHILPATSLTYTDGLAVAQYINSTTTPVALITPVETQLGVKPSPVMAGFSSRGPN 389

Query: 374  PITDSMIKPDITAPGVSILASVSESAPATQFPFDTRRVPFNFETGTSMACPHISGVVGLL 433
            PITD+M+KPDIT PGV+ILASV+    AT+FPFDTRRVPFN E+GTSM+CPHI+GV GLL
Sbjct: 390  PITDAMLKPDITGPGVNILASVTTDVTATKFPFDTRRVPFNVESGTSMSCPHIAGVAGLL 449

Query: 434  KTLYPKWSPAAIKSAIMTTAKTRDNSHKSILDYNKVKATPFSYGAGHVHPNNAMNPGLVY 493
            KTLYP WSPAAIKSAIMTTAKTRDNS  ++ D  K KATPF YGAGHV+PNNAM+PGLVY
Sbjct: 450  KTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDTVKAKATPFDYGAGHVNPNNAMDPGLVY 509

Query: 494  DTTMEDYMNFLCAQGYNYTALKKFYNKPFVCPKSFATTDLNYPSISVPKLTAGATVTINR 553
            DTT++DY+NFLCA+GYN   L  F++KPFVC   F  TDLNYPSIS+P+L +GA VT+NR
Sbjct: 510  DTTIDDYLNFLCARGYNAQTLMSFHSKPFVCATPFTLTDLNYPSISIPELKSGAPVTVNR 569

Query: 554  RVKNVGTPGTYVASVKGSSKVSVSVEPSTLQFNSVGEEKAFKVVFHYKGQGQGQGHVFGT 613
            RVKNVGTPGTYVA VK SSK+SV+VEPSTLQFNSVGEEKAFKVVF YKG GQG+G+VFGT
Sbjct: 570  RVKNVGTPGTYVARVKASSKISVTVEPSTLQFNSVGEEKAFKVVFEYKGTGQGKGYVFGT 629

Query: 614  LI---------------------------------------------------------- 673
            LI                                                          
Sbjct: 630  LIWSDNGKHNVRSPINKICNIFKPLKTLFKDKIKSCYKKNNLHLMINLTSHNYKHYLMMK 689

Query: 674  ------------------------------------------------------------ 733
                                                                        
Sbjct: 690  FMIHIIRNIYMIRSKNLSLNLENIAKVNKFVKKLIFEKYNXYIKTPLGIRKQNSYIVYLG 749

Query: 734  -----------------------------CKLAAKESIIYSYNRYINGFAAVLDEKEATA 793
                                          KLAAKESI YSYNRYINGFAA+LDE +A A
Sbjct: 750  SHFFGSNPSIHDVQLATESQYELLESVVGSKLAAKESIFYSYNRYINGFAAILDENQAIA 809

Query: 794  LENDPSVVSIFENEERELHTTRSWNFLGVENDEGIPPNSIWEAARLGEDTIIANLDTGVW 853
            L  +P+VVSIFEN++R+LHTTRSW+FLG+E+DEGIPPNSIW+AAR GEDTII NLDTG W
Sbjct: 810  LARNPNVVSIFENQKRKLHTTRSWSFLGMESDEGIPPNSIWKAARFGEDTIIGNLDTGAW 869

Query: 854  PESKSFNDAGYGPIPSRWRGACVGGANFTCNRKLIGARYFYQGIASAKGILNNIFNTTRD 913
            PESKSFNDAGYGP+PSRW G C GGANFTCN+KLIGARYF +G  +  G ++    T RD
Sbjct: 870  PESKSFNDAGYGPVPSRWMGVCEGGANFTCNKKLIGARYFNKGFEAENGPMSANLTTARD 929

Query: 914  HGGHGSHTLSTAGGNFVPGANIFGYGNGTAKGGSPKARLVAYKVCWPTSSGGCFDADILA 973
              GHGSHTLSTAGGNFVPGAN+FG GNGTAKGGSP+ARL AYKVCWP+ +GGC+DADILA
Sbjct: 930  QEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPRARLAAYKVCWPSFTGGCYDADILA 989

Query: 974  AFEAAISDGVDVLSVSLGGKAQEFSHDPISIGAFHAVQQGIVVVCSGGNHGPIPRTVSNV 1033
            A E+AI DGVDVLS+SLG  A++F+ D +SIGAFHAVQQGIVVVCSGGN GP P TV+NV
Sbjct: 990  AVESAIHDGVDVLSISLGSSARDFASDTLSIGAFHAVQQGIVVVCSGGNDGPTPGTVTNV 1049

Query: 1034 SPWMFTVAGSFLDRDFVNYVVLGNKKQFKSPSLSSGELPEGKYYPLMNAVEAKAANASDN 1093
            SPWM TVA S +DRDFVNYV LGNK+ FK  SLSSG LP GK+YPL++ V+ KA NA+D 
Sbjct: 1050 SPWMITVAASTVDRDFVNYVALGNKRHFKGVSLSSGGLPRGKFYPLVDGVQVKAGNATDK 1109

Query: 1094 LAQICEYGSLDPTKAKGKIIVCHLGDNSRIDKGFEVVRVGGVGMIIVNDKKNGNNVIVDL 1153
            LA +CE GSLDP KAKGKI++C  GD++R+DK FEV R GG+G+I+VNDK++GN++  D 
Sbjct: 1110 LALLCEDGSLDPAKAKGKIVLCLRGDSARMDKSFEVRRAGGIGLILVNDKEDGNDITADP 1169

Query: 1154 HILPASHLNYEDGLSIAHYMNSTKTPVALITHVRTELGIKPSPVIAGFSSKGPNPITDSM 1213
            H LPASHLNY DG++I  Y+NSTK+P+A ITHV+TELGIKPSP++A FSS+GPNPI DSM
Sbjct: 1170 HFLPASHLNYADGIAIFQYINSTKSPMAFITHVKTELGIKPSPMVADFSSRGPNPIIDSM 1229

Query: 1214 IKPDITAPGGSIIASFTEDVTATKSRFDTRRVPFNVQSGTSMACPHISGVVGLLKTLYPT 1273
            IKPDI APG SI+A+F+E  TAT    DTRRV FN +SGTSMACPHISGVVGLLKTLYP 
Sbjct: 1230 IKPDIAAPGVSILAAFSEYATATDFPLDTRRVSFNFESGTSMACPHISGVVGLLKTLYPK 1289

Query: 1274 WSPAAIKSAIMTTANTRDNTMHPILDSTNVKATPFDYGAGQVNPNNAIDPGLVYDTTIDD 1318
            WSPAAIKSAIMTTA TRDN+M  ILD    KATPF YGAG V+PNNAIDPGLVYDTTI+D
Sbjct: 1290 WSPAAIKSAIMTTAKTRDNSMKSILDYNKAKATPFQYGAGHVHPNNAIDPGLVYDTTIED 1349

BLAST of HG10003277 vs. NCBI nr
Match: XP_023005962.1 (uncharacterized protein LOC111498820 [Cucurbita maxima])

HSP 1 Score: 1887.5 bits (4888), Expect = 0.0e+00
Identity = 921/1391 (66.21%), Postives = 1095/1391 (78.72%), Query Frame = 0

Query: 14   GVWPESKSFSDAGYGPVPSRWMGVCEGGSDFTCNKKLIGARYFYKGFEAANGAINATLRT 73
            GVWPES SF D+GYGPVPSRWMG CEGGS+F+CN+KLIGARYFY+G+E  NG +N +   
Sbjct: 148  GVWPESHSFHDSGYGPVPSRWMGACEGGSNFSCNRKLIGARYFYRGYEMINGPLNISSLN 207

Query: 74   ARDQEGHGSHTLSTAGGNFVPGANLFGNANGTAKGGSPKARLAAYKVCWPGGLDGGCFDA 133
            ARD EGHG+HTLSTAGGNFV GAN+FGN NGTAKGG+PKAR+AAYKVCWP    G C DA
Sbjct: 208  ARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTAKGGAPKARVAAYKVCWP---QGKCSDA 267

Query: 134  DVLAAFETAISDGVDVLSVSLGSPARDFASDPLAIGAFHAVQHGIVVVCSGGNDGPSPGT 193
            D+LA  E AISDGVDVLS+SLG+ A+DFA DP+++GAFHA+Q GI+VVCS GNDGP PGT
Sbjct: 268  DLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVGAFHAIQQGIIVVCSAGNDGPLPGT 327

Query: 194  ITNVSPWIFTVAASTIDRDFINYVTLGNKKHFKGASLSSGGLPGHKFYPLMNGVQVKAAN 253
            +TNVSPW+FTV AS+IDR F +YV LGNKK  KG+SLSSGGLP  K YPLMN V  KA+N
Sbjct: 328  VTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSSLSSGGLPRGKLYPLMNSVNAKASN 387

Query: 254  ATDNLALLCEDGSLDPTKAKGKIILCLRGDNARMDKGLEVRRAGGVGLILVNGKEDGTSI 313
            A+D LA LCE+GSLDP KA+GKII+CLRGDN RMDK  EV R GGVG+ILVN K  G+ I
Sbjct: 388  ASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGVGMILVNDKISGSDI 447

Query: 314  TADPHLLPASHLNYADGLAIFQYVNSTKAPMALITHVTTELGLKPSPMMGDFSSRGPNPI 373
              D H+LP SH++Y DGL+I +Y+ STK P+A IT V TE+G+KPSP+M  FSSRGPN I
Sbjct: 448  ETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITPVRTEIGIKPSPVMAPFSSRGPNHI 507

Query: 374  TDSMIKPDITAPGVSILASVSESAPATQFPFDTRRVPFNFETGTSMACPHISGVVGLLKT 433
             ++MIKPDI+APGV+I+AS +++  AT  PFD RRVPFN ++GTSM+CPHI+GV GLLK 
Sbjct: 508  AEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRVPFNVQSGTSMSCPHIAGVAGLLKK 567

Query: 434  LYPKWSPAAIKSAIMTTAKTRDNSHKSILDYNKVKATPFSYGAGHVHPNNAMNPGLVYDT 493
            L+P WSPAAIKSAIMTTAKTRDN+  ++LD+NKVKATPF YGAGHVHPN+AM+PGLVYDT
Sbjct: 568  LHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAGHVHPNDAMDPGLVYDT 627

Query: 494  TMEDYMNFLCAQGYNYTALKKFYNKPFVCPKSFATTDLNYPSISVPKLTAGATVTINRRV 553
            T++DY+NFLC QGYN   LKKF NKPFVC K+FA+TDLNYPSISVPKL  G  VT+NRRV
Sbjct: 628  TIDDYLNFLCMQGYNSLTLKKFSNKPFVCAKNFASTDLNYPSISVPKLQIGVPVTVNRRV 687

Query: 554  KNVGTPGTYVASVKGSSKVSVSVEPSTLQFNSVGEEKAFKVVFHYKGQGQGQGHVFGTLI 613
            KNVG+ GTYVA V+    ++V VEPSTLQF+SVGEEKAFK+VFHY  + +  G+VFG L+
Sbjct: 688  KNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEEKAFKLVFHYAQKVRRPGYVFGALV 747

Query: 614  ------------------------------------------------------------ 673
                                                                        
Sbjct: 748  WSDGKHFVRSPIAVNLVNFKAKAMEEYNKISPLLLFFFMLHTAAVPTKNSYIVYLGSHSF 807

Query: 674  -------------------------CKLAAKESIIYSYNRYINGFAAVLDEKEATALEND 733
                                      K+AAK+SI+YSYNRYINGFAAVLDE+EATAL  +
Sbjct: 808  APNPSVYDVQLATESQYDILGSVKGSKVAAKDSILYSYNRYINGFAAVLDEQEATALAKN 867

Query: 734  PSVVSIFENEERELHTTRSWNFLGVENDEGIPPNSIWEAARLGEDTIIANLDTGVWPESK 793
            PSVVS+FEN+ER+LHTTRSW FLGV++D GIP NSIW+AAR G DTII NLDTGVWPES 
Sbjct: 868  PSVVSVFENKERKLHTTRSWGFLGVDSDRGIPQNSIWKAARFGADTIIGNLDTGVWPESP 927

Query: 794  SFNDAGYGPIPSRWRGACVGGANFTCNRKLIGARYFYQGIASAKGIL---NNIFNTTRDH 853
            SFNDAGYGP+PSRWRGAC GG+ F CNRKLIGARYFY+G  +A+G L   N  F++ RDH
Sbjct: 928  SFNDAGYGPVPSRWRGACEGGSKFRCNRKLIGARYFYRGFQAAEGPLTTHNISFDSARDH 987

Query: 854  GGHGSHTLSTAGGNFVPGANIFGYGNGTAKGGSPKARLVAYKVCWPTSSGGCFDADILAA 913
             GHGSHTLSTAGGNFV G N+FG GNGTAKGGSP+AR+VAYKVCWP+ +GGC+D+DILA 
Sbjct: 988  EGHGSHTLSTAGGNFVHGVNVFGNGNGTAKGGSPRARVVAYKVCWPSKNGGCYDSDILAG 1047

Query: 914  FEAAISDGVDVLSVSLGGKAQEFSHDPISIGAFHAVQQGIVVVCSGGNHGPIPRTVSNVS 973
             EAAISDGVDVLS S+G  AQEF++D ISIGAFHAVQ GIVVVCS GN GP P +VSNVS
Sbjct: 1048 IEAAISDGVDVLSASIGTLAQEFANDAISIGAFHAVQHGIVVVCSAGNDGPSPGSVSNVS 1107

Query: 974  PWMFTVAGSFLDRDFVNYVVLGNKKQFKSPSLSSGELPEGKYYPLMNAVEAKAANASDNL 1033
            PWM TV  S +DRDFV+YVVLGNKK+F+  SLSS  LP GK+YPL+ AV+ KAANA+D  
Sbjct: 1108 PWMVTVGASTIDRDFVSYVVLGNKKRFRGSSLSSSRLPAGKFYPLIKAVQVKAANATDGF 1167

Query: 1034 AQICEYGSLDPTKAKGKIIVCHLGDNSRIDKGFEVVRVGGVGMIIVNDKKNGNNVIVDLH 1093
            AQ+C  G+LDPTKAKGKIIVC  G+N+R+ KGFEV RVGGVGM++VN++ +G+ ++ D H
Sbjct: 1168 AQLCMDGTLDPTKAKGKIIVCLRGENARVSKGFEVFRVGGVGMVLVNNQMDGSALVADPH 1227

Query: 1094 ILPASHLNYEDGLSIAHYMNSTKTPVALITHVRTELGIKPSPVIAGFSSKGPNPITDSMI 1153
            ILPASHL+Y DG+SIA Y++STKTPVA ITH  TE+GIKPSP++A FSS+GP+ IT ++I
Sbjct: 1228 ILPASHLSYADGVSIAQYLSSTKTPVASITHASTEMGIKPSPLMASFSSRGPDFITQAVI 1287

Query: 1154 KPDITAPGGSIIASFTEDVTATKSRFDTRRVPFNVQSGTSMACPHISGVVGLLKTLYPTW 1213
            KPDITAPG +IIAS T+D++A+    D RRVPFN++SGTSM+CPHISGV GLLKTL+PTW
Sbjct: 1288 KPDITAPGVNIIASVTDDISASGLPLDKRRVPFNIESGTSMSCPHISGVAGLLKTLHPTW 1347

Query: 1214 SPAAIKSAIMTTANTRDNTMHPILDSTNVKATPFDYGAGQVNPNNAIDPGLVYDTTIDDY 1273
            SPAAIKSA+MTTA TRDNT + +LD T VKATPFDYGAG V+PN+A+DPGLVYDTT+DDY
Sbjct: 1348 SPAAIKSAVMTTAKTRDNTKNTLLDYTKVKATPFDYGAGHVHPNDAMDPGLVYDTTVDDY 1407

Query: 1274 LNFLCARGYNYTQLKKFSNKPFVCAESFAITDLNYPSISVPKLTIDAPMTINRRVKNVGS 1317
            LNFLC RGYN   LKKFSNKPFVCA +FA TD NYPSI VP+L I   +T+NRRVKNVGS
Sbjct: 1408 LNFLCTRGYNSRTLKKFSNKPFVCANNFATTDFNYPSILVPRLQIGGSVTVNRRVKNVGS 1467

BLAST of HG10003277 vs. NCBI nr
Match: XP_031738719.1 (uncharacterized protein LOC101212014 [Cucumis sativus])

HSP 1 Score: 1807.7 bits (4681), Expect = 0.0e+00
Identity = 880/1361 (64.66%), Postives = 1052/1361 (77.30%), Query Frame = 0

Query: 14   GVWPESKSFSDAGYGPVPSRWMGVCEGGSDFTCNKKLIGARYFYKGFEAANGAINATLRT 73
            GVWPESKSF+DAGYGPVPSRW G CEGG++F CN+KLIGARYF KGF  A+G +N +  T
Sbjct: 179  GVWPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLNISFNT 238

Query: 74   ARDQEGHGSHTLSTAGGNFVPGANLFGNANGTAKGGSPKARLAAYKVCWPGGLDGGCFDA 133
            ARD++GHGSHTLSTAGGNFVPGAN+FG  NGTAKGGSPKAR+AAYKVCWP    GGC+DA
Sbjct: 239  ARDKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSGGGCYDA 298

Query: 134  DVLAAFETAISDGVDVLSVSLGSPARDFASDPLAIGAFHAVQHGIVVVCSGGNDGPSPGT 193
            D+LA FE AISDGVDVLSVSLGS   +FA D ++IGAFHAVQ GIVVVCS GNDGP PGT
Sbjct: 299  DILAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGPGPGT 358

Query: 194  ITNVSPWIFTVAASTIDRDFINYVTLGNKKHFKGASLSSGGLPGHKFYPLMNGVQVKAAN 253
            ++N+SPW+FTVAAS+IDRDF +Y +LGNKKH+KG+S+SS  L G KFYPL+N V  KAAN
Sbjct: 359  VSNISPWMFTVAASSIDRDFTSYASLGNKKHYKGSSISSSALAGGKFYPLINAVDAKAAN 418

Query: 254  ATDNLALLCEDGSLDPTKAKGKIILCLRGDNARMDKGLEVRRAGGVGLILVNGKEDGTSI 313
            A++ LA LC  GSLDPTKAKGKII+CLRG+NAR++KG  V +AGGVG+ILVNGK  G+  
Sbjct: 419  ASEILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGKNGGSGT 478

Query: 314  TADPHLLPASHLNYADGLAIFQYVNSTKAPMALITHVTTELGLKPSPMMGDFSSRGPNPI 373
            TAD H+LPA+HL+Y DGLA+ QY+NSTK P+A IT V T+LG+KPSP+M DFSSRGPNPI
Sbjct: 479  TADAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSSRGPNPI 538

Query: 374  TDSMIKPDITAPGVSILASVSESAPATQFPFDTRRVPFNFETGTSMACPHISGVVGLLKT 433
            T++M+KPDIT PG+SILASV+    AT FPFDTRRVPFN E+GTSM+CPHISGVVGLLKT
Sbjct: 539  TEAMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGVVGLLKT 598

Query: 434  LYPKWSPAAIKSAIMTTAKTRDNSHKSILDYNKVKATPFSYGAGHVHPNNAMNPGLVYDT 493
            LYP WSPAAIKSAIMTTAKTRDN+ ++I D  K KATPF YGAGHVHPN+AM+PGLVYDT
Sbjct: 599  LYPTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDT 658

Query: 494  TMEDYMNFLCAQGYNYTALKKFYNKPFVCPKSFATTDLNYPSISVPKLTAGATVTINRRV 553
            T++DY+NFLCA+GYN    K FYNKPFVC KSF  TDLNYPSIS+PKL  GA VT+NRRV
Sbjct: 659  TIDDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRV 718

Query: 554  KNVGTPGTYVASVKGSSKVSVSVEPSTLQFNSVGEEKAFKVVFHYKGQGQGQGHVFGTLI 613
            KNVGTPGTYVA V  SSK+ V+VEPSTLQFNSVGEEKAFKVVF YKG  Q +G+VFGTLI
Sbjct: 719  KNVGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLI 778

Query: 614  --------------------------------------------------------CKLA 673
                                                                     K  
Sbjct: 779  WSDGKHNVRSPIVVNLGSYIVYLGSEHSSALDPSSLSEHSRQVTALHYDLLGSLFGSKTM 838

Query: 674  AKESIIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTTRSWNFLGVENDE 733
            A+E+I YSY R  NGFAA LD+KEA  L  +P V+S+FEN+ R+LHTTRSWNFLGVEND 
Sbjct: 839  AEEAIFYSYTRSFNGFAAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDI 898

Query: 734  GIPPNSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACVGGANFTCNRK 793
            GIP NSIW  A+ GED I+AN+DTGVWPESKSF+D GYGP+PS+WRG C   + F CNRK
Sbjct: 899  GIPSNSIWNTAKFGEDVIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRK 958

Query: 794  LIGARYFYQGIASAKGILNNIFNTTRDHGGHGSHTLSTAGGNFVPGANIFGYGNGTAKGG 853
            LIG RYF++G  +A G LN    T RDH GHG+HTLSTA GNFV GAN+FG+GNGTAKGG
Sbjct: 959  LIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGG 1018

Query: 854  SPKARLVAYKVCW-PTSSGGCFDADILAAFEAAISDGVDVLSVSLGGKAQEFSHDPISIG 913
            +PKAR VAYK CW P     CFDADILAAFEAAI+DGVDVLS SLGG A E+ +DP++I 
Sbjct: 1019 APKARAVAYKACWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIA 1078

Query: 914  AFHAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVVLGNKKQFKSPS 973
            AF AVQ+GI+VV SGGN GP P T++N+SPW+FTVA S +DR+F +YV LGNKK  K  S
Sbjct: 1079 AFLAVQRGILVVFSGGNSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLS 1138

Query: 974  LSSGELPEGKYYPLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIVCHLGDNSRIDK 1033
            LSS      K++PL+N+V+AK  N ++  AQ C  G+LDP K KGKI++C +G+   +DK
Sbjct: 1139 LSSVPSLPKKFFPLINSVDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDK 1198

Query: 1034 GFEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMNSTKTPVALITH 1093
            GF+  R G VG+II ND + G+ +  +LH +PAS +   D   + +Y+ ST+TP+A +T 
Sbjct: 1199 GFQASRAGAVGVIIANDLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTS 1258

Query: 1094 VRTELGIKPSPVIAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVTATKSRFDTRRV 1153
            V+T L +KP+P IA FS++GPNPI  +++KPD+TAPG +I+AS+   +  T S  D RR+
Sbjct: 1259 VKTLLSVKPAPTIATFSARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRI 1318

Query: 1154 PFNVQSGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTMHPILDSTNVKA 1213
            PFNV SGTSM+CPH++G+ GL+K+++P WSPAAIKSAIMTTA TR N    ILDST +KA
Sbjct: 1319 PFNVISGTSMSCPHVAGIAGLIKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKA 1378

Query: 1214 TPFDYGAGQVNPNNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFSNKPFVCAESFAIT 1273
            TP+ YGAGQVNPN+A DPGLVYD T++DYLNFLCARGYN  Q+KKF  KPF C  SF +T
Sbjct: 1379 TPYAYGAGQVNPNDAADPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVT 1438

Query: 1274 DLNYPSISVPKLTIDAPMTINRRVKNVGSPGTYVANVEVPEGVVVTVEPNTLQFNNVGEE 1318
            DLNYPSISV +L I AP+T+NRRVKNVGSPGTYVA V+   GV V++EP+TL F+ VGEE
Sbjct: 1439 DLNYPSISVGELKIGAPLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEE 1498

BLAST of HG10003277 vs. NCBI nr
Match: KAG6577540.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1674.1 bits (4334), Expect = 0.0e+00
Identity = 833/1349 (61.75%), Postives = 998/1349 (73.98%), Query Frame = 0

Query: 14   GVWPESKSFSDAGYGPVPSRWMGVCEGGSDFTCNKKLIGARYFYKGFEAANGAINATLRT 73
            GVWPESKSF+D GYG +PS+W G CEGGS+F CN+KLIGARYF KG+ A  G+++ +  T
Sbjct: 147  GVWPESKSFNDEGYGAIPSKWRGSCEGGSNFHCNRKLIGARYFNKGYTALAGSLDDSFDT 206

Query: 74   ARDQEGHGSHTLSTAGGNFVPGANLFGNANGTAKGGSPKARLAAYKVCWPGGLDGGCFDA 133
             RD +GHG+HTLSTAGGNFV GAN+FGN NGTAKGGSPKA +AAYKVCWP    G C DA
Sbjct: 207  TRDHDGHGTHTLSTAGGNFVSGANVFGNGNGTAKGGSPKAFVAAYKVCWPTFHGGQCSDA 266

Query: 134  DVLAAFETAISDGVDVLSVSLGSPARDFASDPLAIGAFHAVQHGIVVVCSGGNDGPSPGT 193
            D+LAA E AI+DGVDVLS+SLG  + +F  D  AIG+FHAVQ GIVVVCSGGN GP P +
Sbjct: 267  DILAAIEAAITDGVDVLSLSLGRGSTEFFDDVTAIGSFHAVQQGIVVVCSGGNSGPDPQS 326

Query: 194  ITNVSPWIFTVAASTIDRDFINYVTLGNKKHFKGASLSSGGLPGHKFYPLMNGVQVKAAN 253
            + NV+PW+FTVAASTI R   +YV LGNKKH  GAS+S   LP  +FYPL+  V  KA N
Sbjct: 327  VENVAPWLFTVAASTITRQLTSYVALGNKKHITGASISDKILPAQQFYPLITSVDAKAIN 386

Query: 254  ATDNLALLCEDGSLDPTKAKGKIILCLR-GDNARMDKGLEVRRAGGVGLILVNGKEDGTS 313
             +   A LC +GSLDP K KGKII+C+R GD+AR+DKG    +AG VG+IL N +EDG  
Sbjct: 387  ISVETAKLCVEGSLDPRKVKGKIIVCVRGGDSARVDKGYVAAQAGAVGMILANSEEDGNE 446

Query: 314  ITADPHLLPASHLNYADGLAIFQYVNSTKAPMALITHVTTELGLKPSPMMGDFSSRGPNP 373
            + AD HLLP SH++Y DG  +++Y+NSTK P+A +THV TE G+KP+P+M  FSSRGPN 
Sbjct: 447  LIADAHLLPVSHISYIDGETVYEYINSTKTPVAYMTHVRTETGIKPAPVMASFSSRGPNS 506

Query: 374  ITDSMIKPDITAPGVSILASVSESAPATQFPFDTRRVPFNFETGTSMACPHISGVVGLLK 433
            I +S++KPDITAPGV+I+A+ SE A  +  PFD RR+PFN  +GTSM+CPHISG+V LLK
Sbjct: 507  IEESILKPDITAPGVNIIAAYSEDASPSGSPFDNRRIPFNVVSGTSMSCPHISGIVALLK 566

Query: 434  TLYPKWSPAAIKSAIMTTAKTRDNSHKSILDYNKVKATPFSYGAGHVHPNNAMNPGLVYD 493
            T+Y KWSPAAIKSAIMTTA+TR N    ILD  K+ A P +YGAGHVHPN A NPGLVYD
Sbjct: 567  TIYSKWSPAAIKSAIMTTAETRANDLHPILDSTKLVANPLAYGAGHVHPNRAANPGLVYD 626

Query: 494  TTMEDYMNFLCAQGYNYTALKKFYNKPFVCPKSFATTDLNYPSISVPKLTAGATVTINRR 553
             T  DY+NFLCA+GYN T L KF N  FVC KSF  TD NYPSIS+PK+ +   VTI R 
Sbjct: 627  LTTNDYLNFLCARGYNKTQLSKFSNTSFVCSKSFKLTDFNYPSISIPKMKS-EPVTIKRT 686

Query: 554  VKNVGTPGTYVASVKGSSKVSVSVEPSTLQFNSVGEEKAFKVVFHYKGQGQGQGHVFGTL 613
            VKNVG+P TYVA VK    V VSVEPSTL+F    EEK FKVVF      + +G+VFG+L
Sbjct: 687  VKNVGSPSTYVARVKVPPGVLVSVEPSTLKFTRTDEEKTFKVVFRSVANNKHRGYVFGSL 746

Query: 614  ----------ICKLA---------------------------------AKESIIYSYNRY 673
                      I  L                                  AKE+I YSYNRY
Sbjct: 747  KWLDGKHHSYIVYLGSHSHGSNPSSADLRVATESHYSLLGSLLGSNEMAKEAIFYSYNRY 806

Query: 674  INGFAAVLDEKEATALENDPSVVSIFENEERELHTTRSWNFLGVENDEGIPPNSIWEAAR 733
            INGFAAVLD   A  L   P+VVS+ EN+ R+LHTT SW FL +EN  G PPNSIW  + 
Sbjct: 807  INGFAAVLDHTVAQDLARHPAVVSVQENKMRKLHTTNSWEFLELENGGGTPPNSIWNVSN 866

Query: 734  LGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACVGGANFTCNRKLIGARYFYQGIA 793
             GE TIIANLDTGVWPESKSF+D GYG IPSRWRG+C GG+NF CNRKLIGARYF +G+ 
Sbjct: 867  FGESTIIANLDTGVWPESKSFSDEGYGAIPSRWRGSCEGGSNFHCNRKLIGARYFNKGVV 926

Query: 794  SAKGILNNIFNTTRDHGGHGSHTLSTAGGNFVPGANIFGYGNGTAKGGSPKARLVAYKVC 853
            +  G L+  F+T RD  GHG+HTLSTAGG+FV GAN+FGYGNGTAKGGSPKA + AYKVC
Sbjct: 927  ALAGSLDVSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYGNGTAKGGSPKALVAAYKVC 986

Query: 854  WPTSSGG-CFDADILAAFEAAISDGVDVLSVSLGGKAQEFSHDPISIGAFHAVQQGIVVV 913
            W T  GG C DADILAA EAAI+DGVDVLS+SLG    EF +D  +IGAFHAVQQGIVVV
Sbjct: 987  WRTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGPMEFFNDVAAIGAFHAVQQGIVVV 1046

Query: 914  CSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVVLGNKKQFKSPSLSSGELPEGKYY 973
            CSGGN GP P+++ NV+PW+FTVA S ++R F +YV LGN+K     SLS   LP  ++Y
Sbjct: 1047 CSGGNSGPDPQSIENVAPWLFTVAASTINRQFTSYVALGNEKNITGESLSDKILPAQQFY 1106

Query: 974  PLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIVCHLGDNSRIDKGFEVVRVGGVGM 1033
            PL+ + +AKA N S  +A++C  GSLDP K KGKIIVC  GD++R+DKGF   + G VGM
Sbjct: 1107 PLITSADAKANNVSVEIAKLCVEGSLDPRKVKGKIIVCVRGDDARVDKGFVAAKAGAVGM 1166

Query: 1034 IIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMNSTKTPVALITHVRTELGIKPSPV 1093
            I+ N++K GN+V+ D HILPASH++Y DG ++  Y+NSTKTPVA +THVRTE GIKP+PV
Sbjct: 1167 ILANNEKYGNDVVADAHILPASHISYIDGETVYKYINSTKTPVAYMTHVRTETGIKPAPV 1226

Query: 1094 IAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVTATKSRFDTRRVPFNVQSGTSMAC 1153
            +A FSS+GP+ I +S++KPDITAPG +IIA+++ED + + S FD RR+PFNV SGTSM+C
Sbjct: 1227 MASFSSRGPSSIEESILKPDITAPGINIIAAYSEDASPSGSPFDNRRIPFNVVSGTSMSC 1286

Query: 1154 PHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTMHPILDSTNVKATPFDYGAGQVNP 1213
            PHI+G+V LLKT+YP WSPAAIKSAIMTTA TR N +HPILD T + A P  YGAG V P
Sbjct: 1287 PHIAGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDFTKLVANPLAYGAGHVQP 1346

Query: 1214 NNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFSNKPFVCAESFAITDLNYPSISVPKL 1273
            N A +PGLVYD T +DYLNFLCARGYN  QL KFSN  FVC++SF +TD NYPSIS+P +
Sbjct: 1347 NRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVCSKSFKLTDFNYPSISIPNM 1406

Query: 1274 TIDAPMTINRRVKNVGSPGTYVANVEVPEGVVVTVEPNTLQFNNVGEEKAFKVVFRYIGE 1318
                PMTI R VKNVGSP TYVA VEVP GV+V+VEPNTL+F    EEK FKVVFR +  
Sbjct: 1407 K-SGPMTIKRTVKNVGSPSTYVAQVEVPPGVLVSVEPNTLKFTRTDEEKTFKVVFRSVAN 1466

BLAST of HG10003277 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 6.9e-235
Identity = 401/703 (57.04%), Postives = 511/703 (72.69%), Query Frame = 0

Query: 618  AKESIIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTTRSWNFLGVENDE 677
            A ++I YSY ++INGFAA LD   A  +   P VVS+F N+  +LHTTRSW+FLG+E++ 
Sbjct: 71   ATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNS 130

Query: 678  GIPPNSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACVG--GANFTCN 737
             +P +SIW  AR GEDTIIANLDTGVWPESKSF D G GPIPSRW+G C     A F CN
Sbjct: 131  YVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCN 190

Query: 738  RKLIGARYFYQGIASAKGILNNIFNTTRDHGGHGSHTLSTAGGNFVPGANIFGYGNGTAK 797
            RKLIGARYF +G A+A G LN+ F++ RD  GHGSHTLSTA G+FVPG +IFG GNGTAK
Sbjct: 191  RKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAK 250

Query: 798  GGSPKARLVAYKVCWPTSSGG-CFDADILAAFEAAISDGVDVLSVSLGGKAQEFSHDPIS 857
            GGSP+AR+ AYKVCWP   G  C+DAD+LAAF+AAI DG DV+SVSLGG+   F +D ++
Sbjct: 251  GGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVA 310

Query: 858  IGAFHAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVVLGNKKQFKS 917
            IG+FHA ++ IVVVCS GN GP   TVSNV+PW  TV  S +DR+F + +VLGN K +K 
Sbjct: 311  IGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKG 370

Query: 918  PSLSSGELPEGKYYPLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIVCHLGDNSRI 977
             SLSS  LP  K+YP+M +V AKA NAS   AQ+C+ GSLDP K KGKI+VC  G N R+
Sbjct: 371  QSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRV 430

Query: 978  DKGFEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMNSTKTPVALI 1037
            +KG  V   GG+GM++ N    GN+++ D H+LPA+ L  +D  +++ Y++ TK P+A I
Sbjct: 431  EKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHI 490

Query: 1038 THVRTELGIKPSPVIAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVTATKSRFDTR 1097
            T  RT+LG+KP+PV+A FSSKGP+ +   ++KPDITAPG S+IA++T  V+ T  +FD R
Sbjct: 491  TPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPR 550

Query: 1098 RVPFNVQSGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTMHPILDSTNV 1157
            R+ FN  SGTSM+CPHISG+ GLLKT YP+WSPAAI+SAIMTTA   D+   PI ++TN+
Sbjct: 551  RLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNM 610

Query: 1158 KATPFDYGAGQVNPNNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFSNKPFVCAE-SF 1217
            KATPF +GAG V PN A++PGLVYD  I DYLNFLC+ GYN +Q+  FS   F C+    
Sbjct: 611  KATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKI 670

Query: 1218 AITDLNYPSISVPKLTIDAPMTINRRVKNVGSPGTYVANVEVPEGVVVTVEPNTLQFNNV 1277
            ++ +LNYPSI+VP LT  + +T++R VKNVG P  Y   V  P+GV V V+P +L F  V
Sbjct: 671  SLVNLNYPSITVPNLT-SSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKV 730

Query: 1278 GEEKAFKVVFRYIGEGQREGYMFGTLIWSDGKHFVRSPIAMKL 1317
            GE+K FKV+         +GY+FG L+WSD KH VRSPI +KL
Sbjct: 731  GEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of HG10003277 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 8.4e-225
Identity = 393/702 (55.98%), Postives = 491/702 (69.94%), Query Frame = 0

Query: 618  AKESIIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTTRSWNFLGVENDE 677
            AKE+I YSY R+INGFAA+LDE EA  +   P VVS+F N+ R+LHTT SWNF+ +  + 
Sbjct: 81   AKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNG 140

Query: 678  GIPPNSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACVGGANFTCNRK 737
             +  +S+W  A  GEDTIIANLDTGVWPESKSF+D GYG +P+RW+G C    +  CNRK
Sbjct: 141  VVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRC--HKDVPCNRK 200

Query: 738  LIGARYFYQGIASAKGILNNI-FNTTRDHGGHGSHTLSTAGGNFVPGANIFGYGNGTAKG 797
            LIGARYF +G  +  G+ +N  + T RDH GHGSHTLSTA GNFVPGAN+FG GNGTA G
Sbjct: 201  LIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASG 260

Query: 798  GSPKARLVAYKVCWPTSSGG-CFDADILAAFEAAISDGVDVLSVSLGGKAQEFSHDPISI 857
            GSPKAR+ AYKVCWP   G  CFDADILAA EAAI DGVDVLS S+GG A ++  D I+I
Sbjct: 261  GSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAI 320

Query: 858  GAFHAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVVLGNKKQFKSP 917
            G+FHAV+ G+ VVCS GN GP   TVSNV+PW+ TV  S +DR+F  +V L N + FK  
Sbjct: 321  GSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGT 380

Query: 918  SLSSGELPEGKYYPLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIVCHLGDNSRID 977
            SLS   LPE K Y L++A +A  AN +   A +C+ GSLDP K KGKI+VC  GDN+R+D
Sbjct: 381  SLSK-PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVD 440

Query: 978  KGFEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMNSTKTPVALIT 1037
            KG +    G  GM++ NDK +GN +I D H+LPAS ++Y+DG ++  Y++STK P   I 
Sbjct: 441  KGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIK 500

Query: 1038 HVRTELGIKPSPVIAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVTATKSRFDTRR 1097
                 L  KP+P +A FSS+GPN IT  ++KPDITAPG +IIA+FTE    T    D RR
Sbjct: 501  APTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRR 560

Query: 1098 VPFNVQSGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTMHPILDSTNVK 1157
             PFN +SGTSM+CPHISGVVGLLKTL+P WSPAAI+SAIMTT+ TR+N   P++D +  K
Sbjct: 561  TPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKK 620

Query: 1158 ATPFDYGAGQVNPNNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFSNKP-FVCAESFA 1217
            A PF YG+G V PN A  PGLVYD T  DYL+FLCA GYN T ++ F+  P + C +   
Sbjct: 621  ANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGAN 680

Query: 1218 ITDLNYPSISVPKLTIDAPMTINRRVKNVGSPGTYVANVEVPEGVVVTVEPNTLQFNNVG 1277
            + D NYPSI+VP LT    +T+ R++KNVG P TY A    P GV V+VEP  L FN  G
Sbjct: 681  LLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTG 740

Query: 1278 EEKAFKVVFRYIGEGQREGYMFGTLIWSDGKHFVRSPIAMKL 1317
            E K F++  R +      GY+FG L W+D  H+VRSPI ++L
Sbjct: 741  EVKIFQMTLRPL-PVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of HG10003277 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 665.6 bits (1716), Expect = 1.1e-189
Identity = 355/735 (48.30%), Postives = 473/735 (64.35%), Query Frame = 0

Query: 606  GHVFGTLICKLAAKESIIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTT 665
            G +FG+   +  AKE+IIYSYNR+INGFAA+L+E+EA  +  +P+VVS+F ++E +LHTT
Sbjct: 62   GSIFGS---REKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTT 121

Query: 666  RSWNFLGVENDEGIPPNSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGA 725
            RSW FLG+        NS W+  R GE+TII N+DTGVWPES+SF+D GYG +PS+WRG 
Sbjct: 122  RSWEFLGLHRR---GQNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGG 181

Query: 726  CV------GGANFTCNRKLIGARYFYQGIASAKGILNNIFNTTRDHGGHGSHTLSTAGGN 785
                    G    TCNRKLIGARY+ +   +  G L+ + +T RD  GHG+HTLSTAGGN
Sbjct: 182  LCQINKLPGSMKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGN 241

Query: 786  FVPGANIFGYGNGTAKGGSPKARLVAYKVCWP-TSSGGCFDADILAAFEAAISDGVDVLS 845
            FVPGA +F  GNGTAKGGSP+AR+ AYKVCW  T    C+ AD+LAA + AI DGVDV++
Sbjct: 242  FVPGARVFAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVIN 301

Query: 846  VSLGGK----AQEFSHDPISIGAFHAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGS 905
            VS G      A+    D ISIGAFHA+ + I++V S GN GP P TV+NV+PW+FT+A S
Sbjct: 302  VSFGVSYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAAS 361

Query: 906  FLDRDFVNYVVLGNKKQFKSPSLSSGELPEGKYYPLMNAVEAKAANASDNLAQICEYGSL 965
             LDRDF + + + N  Q    +     LP  + + L+ + +AK ANA+   AQ+C  G+L
Sbjct: 362  TLDRDFSSNLTINN--QLIEGASLFVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTL 421

Query: 966  DPTKAKGKIIVC-HLGDNSRIDKGFEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLN 1025
            D TK  GKI++C   G    + +G E +  G  GMI+ N  +NG  +  + H+    +  
Sbjct: 422  DRTKVNGKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTP 481

Query: 1026 YEDGLSIAHYMNST---------KTPVAL-ITHVRTELGIKPSPVIAGFSSKGPNPITDS 1085
                 S  H + +T         KT   + ++  RT  G KP+PV+A FSS+GPN I  S
Sbjct: 482  PRRAKSRPHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPS 541

Query: 1086 MIKPDITAPGGSIIASFTEDVTATKSRFDTRR-VPFNVQSGTSMACPHISGVVGLLKTLY 1145
            ++KPD+TAPG +I+A+++E  +A+    D RR   FNV  GTSM+CPH SG+ GLLKT +
Sbjct: 542  ILKPDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRH 601

Query: 1146 PTWSPAAIKSAIMTTANTRDNTMHPILDS-TNVKATPFDYGAGQVNPNNAIDPGLVYDTT 1205
            P+WSPAAIKSAIMTTA T DNT  PI D+     A  F YG+G V P+ AI+PGLVYD +
Sbjct: 602  PSWSPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLS 661

Query: 1206 IDDYLNFLCARGYNYTQLKKFS-NKPFVCAESFAITDLNYPSISVPKLTIDAPMTINRRV 1265
            + DYLNFLCA GY+   +   + N+ F+C+ S ++ DLNYPSI++P L +  P+TI R V
Sbjct: 662  LTDYLNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRL-KPVTIARTV 721

Query: 1266 KNVGSPGTYVANVEVPEGVVVTVEPNTLQFNNVGEEKAFKVVFRYIGEGQREGYMFGTLI 1316
             NVG P TY  +   P G  + V P +L F  +GE K FKV+ +      R  Y FG L 
Sbjct: 722  TNVGPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLR 781

BLAST of HG10003277 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.0e-170
Identity = 317/702 (45.16%), Postives = 442/702 (62.96%), Query Frame = 0

Query: 622  IIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTTRSWNFLGVENDEGIPP 681
            ++Y+Y   I+GF+  L ++EA +L   P V+S+      ELHTTR+  FLG++       
Sbjct: 65   LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA--- 124

Query: 682  NSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACVGGANFT---CNRKL 741
              ++  A    D ++  LDTGVWPESKS++D G+GPIPS W+G C  G NFT   CNRKL
Sbjct: 125  -DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 184

Query: 742  IGARYFYQGIASAKGILNNI--FNTTRDHGGHGSHTLSTAGGNFVPGANIFGYGNGTAKG 801
            IGAR+F +G  S  G ++      + RD  GHG+HT STA G+ V GA++ GY +GTA+G
Sbjct: 185  IGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARG 244

Query: 802  GSPKARLVAYKVCWPTSSGGCFDADILAAFEAAISDGVDVLSVSLGGKAQEFSHDPISIG 861
             +P+AR+  YKVCW    GGCF +DILAA + AI+D V+VLS+SLGG   ++  D ++IG
Sbjct: 245  MAPRARVAVYKVCW---LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIG 304

Query: 862  AFHAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVVLGNKKQFKSPS 921
            AF A+++GI+V CS GN GP   ++SNV+PW+ TV    LDRDF    +LGN K F   S
Sbjct: 305  AFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVS 364

Query: 922  LSSGELPEGKYYPLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIVCHLGDNSRIDK 981
            L  GE    K  P + A    A+NA++    +C  G+L P K KGKI++C  G N+R+ K
Sbjct: 365  LFKGEALPDKLLPFIYA--GNASNATN--GNLCMTGTLIPEKVKGKIVMCDRGINARVQK 424

Query: 982  GFEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMNSTKTPVALITH 1041
            G  V   GGVGMI+ N   NG  ++ D H+LPA+ +  + G  I HY+ +   P A I+ 
Sbjct: 425  GDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISI 484

Query: 1042 VRTELGIKPSPVIAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVTATKSRFDTRRV 1101
            + T +G+KPSPV+A FSS+GPN IT +++KPD+ APG +I+A++T     T    D+RRV
Sbjct: 485  LGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 544

Query: 1102 PFNVQSGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTMHPILD-STNVK 1161
             FN+ SGTSM+CPH+SG+  LLK+++P WSPAAI+SA+MTTA        P+LD +T   
Sbjct: 545  EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKP 604

Query: 1162 ATPFDYGAGQVNPNNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFSNKPFVC--AESF 1221
            +TPFD+GAG V+P  A +PGL+YD T +DYL FLCA  Y   Q++  S + + C  ++S+
Sbjct: 605  STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 664

Query: 1222 AITDLNYPSISVPKLTIDAPMTINRRVKNVGSPGTYVANV-EVPEGVVVTVEPNTLQFNN 1281
            ++ DLNYPS +V    + A     R V +VG  GTY   V     GV ++VEP  L F  
Sbjct: 665  SVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKE 724

Query: 1282 VGEEKAFKVVFRYIGEGQREGYMFGTLIWSDGKHFVRSPIAM 1315
              E+K++ V F            FG++ WSDGKH V SP+A+
Sbjct: 725  ANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of HG10003277 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 6.1e-159
Identity = 305/705 (43.26%), Postives = 428/705 (60.71%), Query Frame = 0

Query: 622  IIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTTRSWNFLGVENDEGIPP 681
            I+Y+Y    +G AA L ++EA  LE +  VV++      ELHTTRS  FLG+E  E    
Sbjct: 79   ILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQES--- 138

Query: 682  NSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACVGGANF---TCNRKL 741
              +W       D ++  LDTG+WPES+SFND G  P+P+ WRGAC  G  F    CNRK+
Sbjct: 139  ERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKI 198

Query: 742  IGARYFYQGIASAKGILNN--IFNTTRDHGGHGSHTLSTAGGNFVPGANIFGYGNGTAKG 801
            +GAR FY+G  +A G ++    + + RD  GHG+HT +T  G+ V GAN+FG+  GTA+G
Sbjct: 199  VGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARG 258

Query: 802  GSPKARLVAYKVCWPTSSGGCFDADILAAFEAAISDGVDVLSVSLGGKAQEFSHDPISIG 861
             + KAR+ AYKVCW    GGCF +DIL+A + A++DGV VLS+SLGG    +S D +SI 
Sbjct: 259  MAQKARVAAYKVCW---VGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIA 318

Query: 862  AFHAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVVLGNKKQFKSPS 921
             F A++ G+ V CS GN GP P +++NVSPW+ TV  S +DRDF   V +G  + FK  S
Sbjct: 319  TFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVS 378

Query: 922  LSSGE--LPEGKYYPLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIVCHLGDNSRI 981
            L  G   LP+ K YPL   V      +S +    C  G+LD     GKI++C  G   R+
Sbjct: 379  LYKGRTVLPKNKQYPL---VYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRV 438

Query: 982  DKGFEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMNSTKTPVALI 1041
             KG  V R GG+GM++ N   NG  ++ D H+LPA  +  ++G  I  Y  ++K   A +
Sbjct: 439  QKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASL 498

Query: 1042 THVRTELGIKPSPVIAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVTATKSRFDTR 1101
              + T +GIKPSPV+A FSS+GPN ++  ++KPD+ APG +I+A++T D+  +    D R
Sbjct: 499  EILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPR 558

Query: 1102 RVPFNVQSGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTMHPILDSTN- 1161
            RV FN+ SGTSM+CPH+SGV  L+K+ +P WSPAAIKSA+MTTA   DN   P+ D++  
Sbjct: 559  RVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGA 618

Query: 1162 VKATPFDYGAGQVNPNNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFS-NKPFVCAES 1221
              ++P+D+GAG ++P  A DPGLVYD    +Y  FLC +  + +QLK F+ +    C  +
Sbjct: 619  APSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHT 678

Query: 1222 FAIT--DLNYPSISV--PKLTIDAPMTINRRVKNVGSP-GTYVANVEVPEGVVVTVEPNT 1281
             A    +LNYP+IS   P+ T    MT+ R V NVG    +Y  +V   +G  VTV+P T
Sbjct: 679  LAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKT 738

Query: 1282 LQFNNVGEEKAFKVVFRYIGEGQREGYMFGTLIWSDGKHFVRSPI 1313
            L F +  ++ ++ V FR     +R    FG L+W    H VRSP+
Sbjct: 739  LNFTSKHQKLSYTVTFRTRFRMKRP--EFGGLVWKSTTHKVRSPV 772

BLAST of HG10003277 vs. ExPASy TrEMBL
Match: A0A1S3BLG8 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491134 PE=3 SV=1)

HSP 1 Score: 1938.3 bits (5020), Expect = 0.0e+00
Identity = 941/1360 (69.19%), Postives = 1104/1360 (81.18%), Query Frame = 0

Query: 14   GVWPESKSFSDAGYGPVPSRWMGVCEGGSDFTCNKKLIGARYFYKGFEAANGAINATL-R 73
            GVWPESKSF+DAGYGPVPSRW G CEGG++F CN+KLIGARYF+ GF   +G IN +  +
Sbjct: 152  GVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRKLIGARYFHAGFVMGSGLINVSFNK 211

Query: 74   TARDQEGHGSHTLSTAGGNFVPGANLFGNANGTAKGGSPKARLAAYKVCWPGGL-DGGCF 133
            TARD++GHGSHTLSTAGGNFVPGA++FG  NGTAKGGSPKAR+AAY+VCWP  L  GGC+
Sbjct: 212  TARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAKGGSPKARVAAYRVCWPPALFGGGCY 271

Query: 134  DADVLAAFETAISDGVDVLSVSLGSPARDFASDPLAIGAFHAVQHGIVVVCSGGNDGPSP 193
            DAD+LA FE AISDGVDVLSVSLGS A++F+ D ++IGAFHAV+ GIVVVCS GN GP P
Sbjct: 272  DADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSMSIGAFHAVEQGIVVVCSAGNSGPGP 331

Query: 194  GTITNVSPWIFTVAASTIDRDFINYVTLGNKKHFKGASLSSGGLPGHKFYPLMNGVQVKA 253
             T++NVSPW+FTV ASTIDRDF +Y TLGNKK +KG+SLSS  L G KFYPL+N V  + 
Sbjct: 332  KTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYKGSSLSSSSLAGGKFYPLINAVDARN 391

Query: 254  ANATDNLALLCEDGSLDPTKAKGKIILCLRGDNARMDKGLEVRRAGGVGLILVNGKEDGT 313
             N+TD+ A  CE GSLDP K KGKI++CLRG  AR++KG  V +AGGVG+ILVN K DG+
Sbjct: 392  PNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTARVEKGYVVLQAGGVGMILVNDKTDGS 451

Query: 314  SITADPHLLPASHLNYADGLAIFQYVNSTKAPMALITHVTTELGLKPSPMMGDFSSRGPN 373
             + +D H+LPA+ L Y DGLA+ QY+NST  P+ALIT V T+LG+KPSP+M  FSSRGPN
Sbjct: 452  GLISDSHILPATSLTYTDGLAVAQYINSTTTPVALITPVETQLGVKPSPVMAGFSSRGPN 511

Query: 374  PITDSMIKPDITAPGVSILASVSESAPATQFPFDTRRVPFNFETGTSMACPHISGVVGLL 433
            PITD+M+KPDIT PGV+ILASV+    AT+FPFDTRRVPFN E+GTSM+CPHI+GV GLL
Sbjct: 512  PITDAMLKPDITGPGVNILASVTTDVTATKFPFDTRRVPFNVESGTSMSCPHIAGVSGLL 571

Query: 434  KTLYPKWSPAAIKSAIMTTAKTRDNSHKSILDYNKVKATPFSYGAGHVHPNNAMNPGLVY 493
            KTLYP WSPAAIKSAIMTTAKTRDNS  ++ D  K KATPF YGAGHV+PNNAM+PGLVY
Sbjct: 572  KTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDTVKAKATPFDYGAGHVNPNNAMDPGLVY 631

Query: 494  DTTMEDYMNFLCAQGYNYTALKKFYNKPFVCPKSFATTDLNYPSISVPKLTAGATVTINR 553
            DTT++DY+NFLCA+GYN   L  F++KPFVC   F  TDLNYPSIS+P+L +GA VT+NR
Sbjct: 632  DTTIDDYLNFLCARGYNAQTLMSFHSKPFVCATPFTLTDLNYPSISIPELKSGAPVTVNR 691

Query: 554  RVKNVGTPGTYVASVKGSSKVSVSVEPSTLQFNSVGEEKAFKVVFHYKGQGQGQGHVFGT 613
            RVKNVGTPGTYVA VK SSK+SV+VEPSTLQFNSVGEEKAFKV+F YKG GQG+ +VFGT
Sbjct: 692  RVKNVGTPGTYVARVKASSKISVTVEPSTLQFNSVGEEKAFKVLFEYKGTGQGKSYVFGT 751

Query: 614  LI------------------------------------------------------CKLA 673
            LI                                                       KLA
Sbjct: 752  LIWSDNGKHNVRSPIVVKLGSYIVYLGSHFFGSNPSIHDVQLATESQYELLESVVGSKLA 811

Query: 674  AKESIIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTTRSWNFLGVENDE 733
            AKESI YSYNRYINGFAA+LDE +A AL  +P+VVSIFEN++R+LHTTRSW+FLG+E+DE
Sbjct: 812  AKESIFYSYNRYINGFAAILDENQAIALARNPNVVSIFENQKRKLHTTRSWSFLGMESDE 871

Query: 734  GIPPNSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACVGGANFTCNRK 793
            GIPPNSIW+AAR GEDTII NLDTG WPESKSFNDAGYGP+PSRW G C GGANFTCN+K
Sbjct: 872  GIPPNSIWKAARFGEDTIIGNLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGANFTCNKK 931

Query: 794  LIGARYFYQGIASAKGILNNIFNTTRDHGGHGSHTLSTAGGNFVPGANIFGYGNGTAKGG 853
            LIGARYF +G  +  G ++    T RD  GHGSHTLSTAGGNFVPGAN+FG GNGTAKGG
Sbjct: 932  LIGARYFNKGFEAENGPMSANLTTARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGG 991

Query: 854  SPKARLVAYKVCWPTSSGGCFDADILAAFEAAISDGVDVLSVSLGGKAQEFSHDPISIGA 913
            SP+ARL AYKVCWP+ +GGC+DADILAA E+AI DGVDVLS+SLG  A++F+ D +SIGA
Sbjct: 992  SPRARLAAYKVCWPSFTGGCYDADILAAVESAIHDGVDVLSISLGSSARDFASDTLSIGA 1051

Query: 914  FHAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVVLGNKKQFKSPSL 973
            FHAVQQGIVVVCSGGN GP P TV+NVSPWM TVA S +DRDFVNYV LGNK+ FK  SL
Sbjct: 1052 FHAVQQGIVVVCSGGNDGPTPGTVTNVSPWMITVAASTVDRDFVNYVALGNKRHFKGVSL 1111

Query: 974  SSGELPEGKYYPLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIVCHLGDNSRIDKG 1033
            SSG LP GK+YPL++ V+ KA NA+D LA +CE GSLDP KAKGKI++C  GD++R+DK 
Sbjct: 1112 SSGGLPRGKFYPLVDGVQVKAGNATDKLALLCEDGSLDPAKAKGKIVLCLRGDSARMDKS 1171

Query: 1034 FEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMNSTKTPVALITHV 1093
            FEV R GG+G+I+VNDK++GN++  D H LPASHLNY DG++I  Y+NSTK+P+A ITHV
Sbjct: 1172 FEVRRAGGIGLILVNDKEDGNDITADPHFLPASHLNYADGIAIFQYINSTKSPMAFITHV 1231

Query: 1094 RTELGIKPSPVIAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVTATKSRFDTRRVP 1153
            +TE+GIKPSP++A FSS+GPNPI DSMIKPDI APG SI+A+F+E  TAT    DTRRV 
Sbjct: 1232 KTEMGIKPSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSEYATATDFPLDTRRVS 1291

Query: 1154 FNVQSGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTMHPILDSTNVKAT 1213
            FN +SGTSMACPHISGVVGLLKTLYP WSPAAIKSAIMTTA TRDN+M  ILD    KAT
Sbjct: 1292 FNFESGTSMACPHISGVVGLLKTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDYNKAKAT 1351

Query: 1214 PFDYGAGQVNPNNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFSNKPFVCAESFAITD 1273
            PF YGAG V+PNNAIDPGLVYDTTI+DY+NF+CA+GYN T LK+F NKP++C +SF +TD
Sbjct: 1352 PFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKRFYNKPYLCPKSFPLTD 1411

Query: 1274 LNYPSISVPKLTIDAPMTINRRVKNVGSPGTYVANVEVPEGVVVTVEPNTLQFNNVGEEK 1318
            LNYPSISVPKLTI  P+TINRR+KNVG+PGTYVA V+V   V VTV+P+TLQFN+VGEEK
Sbjct: 1412 LNYPSISVPKLTIGVPVTINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEK 1471

BLAST of HG10003277 vs. ExPASy TrEMBL
Match: A0A5D3D732 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00170 PE=3 SV=1)

HSP 1 Score: 1907.9 bits (4941), Expect = 0.0e+00
Identity = 944/1453 (64.97%), Postives = 1105/1453 (76.05%), Query Frame = 0

Query: 14   GVWPESKSFSDAGYGPVPSRWMGVCEGGSDFTCNKKLIGARYFYKGFEAANGAINATL-R 73
            GVWPESKSF+DAGYGPVPSRW G CEGG++F CN+KLIGARYF+ GF   +G IN +  +
Sbjct: 30   GVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRKLIGARYFHAGFVMGSGLINVSFNK 89

Query: 74   TARDQEGHGSHTLSTAGGNFVPGANLFGNANGTAKGGSPKARLAAYKVCWPGGL-DGGCF 133
            TARD++GHGSHTLSTAGGNFVPGA++FG  NGTAKGGSPKAR+AAY+VCWP  L  GGC+
Sbjct: 90   TARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAKGGSPKARVAAYRVCWPPALFGGGCY 149

Query: 134  DADVLAAFETAISDGVDVLSVSLGSPARDFASDPLAIGAFHAVQHGIVVVCSGGNDGPSP 193
            DAD+LA FE AISDGVDVLSVSLGS A++F+ D ++IGAFHAV+ GIVVVCS GN GP P
Sbjct: 150  DADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSMSIGAFHAVEQGIVVVCSAGNSGPGP 209

Query: 194  GTITNVSPWIFTVAASTIDRDFINYVTLGNKKHFKGASLSSGGLPGHKFYPLMNGVQVKA 253
             T++NVSPW+FTV ASTIDRDF +Y TLGNKK +KG+SLSS  L G KFYPL+N V  + 
Sbjct: 210  KTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYKGSSLSSSSLAGGKFYPLINAVDARN 269

Query: 254  ANATDNLALLCEDGSLDPTKAKGKIILCLRGDNARMDKGLEVRRAGGVGLILVNGKEDGT 313
             N+TD+ A  CE GSLDP K KGKI++CLRG  AR++KG  V +AGGVG+ILVN K DG+
Sbjct: 270  PNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTARVEKGYVVLQAGGVGMILVNDKTDGS 329

Query: 314  SITADPHLLPASHLNYADGLAIFQYVNSTKAPMALITHVTTELGLKPSPMMGDFSSRGPN 373
             + +D H+LPA+ L Y DGLA+ QY+NST  P+ALIT V T+LG+KPSP+M  FSSRGPN
Sbjct: 330  GLISDSHILPATSLTYTDGLAVAQYINSTTTPVALITPVETQLGVKPSPVMAGFSSRGPN 389

Query: 374  PITDSMIKPDITAPGVSILASVSESAPATQFPFDTRRVPFNFETGTSMACPHISGVVGLL 433
            PITD+M+KPDIT PGV+ILASV+    AT+FPFDTRRVPFN E+GTSM+CPHI+GV GLL
Sbjct: 390  PITDAMLKPDITGPGVNILASVTTDVTATKFPFDTRRVPFNVESGTSMSCPHIAGVAGLL 449

Query: 434  KTLYPKWSPAAIKSAIMTTAKTRDNSHKSILDYNKVKATPFSYGAGHVHPNNAMNPGLVY 493
            KTLYP WSPAAIKSAIMTTAKTRDNS  ++ D  K KATPF YGAGHV+PNNAM+PGLVY
Sbjct: 450  KTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDTVKAKATPFDYGAGHVNPNNAMDPGLVY 509

Query: 494  DTTMEDYMNFLCAQGYNYTALKKFYNKPFVCPKSFATTDLNYPSISVPKLTAGATVTINR 553
            DTT++DY+NFLCA+GYN   L  F++KPFVC   F  TDLNYPSIS+P+L +GA VT+NR
Sbjct: 510  DTTIDDYLNFLCARGYNAQTLMSFHSKPFVCATPFTLTDLNYPSISIPELKSGAPVTVNR 569

Query: 554  RVKNVGTPGTYVASVKGSSKVSVSVEPSTLQFNSVGEEKAFKVVFHYKGQGQGQGHVFGT 613
            RVKNVGTPGTYVA VK SSK+SV+VEPSTLQFNSVGEEKAFKVVF YKG GQG+G+VFGT
Sbjct: 570  RVKNVGTPGTYVARVKASSKISVTVEPSTLQFNSVGEEKAFKVVFEYKGTGQGKGYVFGT 629

Query: 614  LI---------------------------------------------------------- 673
            LI                                                          
Sbjct: 630  LIWSDNGKHNVRSPINKICNIFKPLKTLFKDKIKSCYKKNNLHLMINLTSHNYKHYLMMK 689

Query: 674  ------------------------------------------------------------ 733
                                                                        
Sbjct: 690  FMIHIIRNIYMIRSKNLSLNLENIAKVNKFVKKLIFEKYNXYIKTPLGIRKQNSYIVYLG 749

Query: 734  -----------------------------CKLAAKESIIYSYNRYINGFAAVLDEKEATA 793
                                          KLAAKESI YSYNRYINGFAA+LDE +A A
Sbjct: 750  SHFFGSNPSIHDVQLATESQYELLESVVGSKLAAKESIFYSYNRYINGFAAILDENQAIA 809

Query: 794  LENDPSVVSIFENEERELHTTRSWNFLGVENDEGIPPNSIWEAARLGEDTIIANLDTGVW 853
            L  +P+VVSIFEN++R+LHTTRSW+FLG+E+DEGIPPNSIW+AAR GEDTII NLDTG W
Sbjct: 810  LARNPNVVSIFENQKRKLHTTRSWSFLGMESDEGIPPNSIWKAARFGEDTIIGNLDTGAW 869

Query: 854  PESKSFNDAGYGPIPSRWRGACVGGANFTCNRKLIGARYFYQGIASAKGILNNIFNTTRD 913
            PESKSFNDAGYGP+PSRW G C GGANFTCN+KLIGARYF +G  +  G ++    T RD
Sbjct: 870  PESKSFNDAGYGPVPSRWMGVCEGGANFTCNKKLIGARYFNKGFEAENGPMSANLTTARD 929

Query: 914  HGGHGSHTLSTAGGNFVPGANIFGYGNGTAKGGSPKARLVAYKVCWPTSSGGCFDADILA 973
              GHGSHTLSTAGGNFVPGAN+FG GNGTAKGGSP+ARL AYKVCWP+ +GGC+DADILA
Sbjct: 930  QEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPRARLAAYKVCWPSFTGGCYDADILA 989

Query: 974  AFEAAISDGVDVLSVSLGGKAQEFSHDPISIGAFHAVQQGIVVVCSGGNHGPIPRTVSNV 1033
            A E+AI DGVDVLS+SLG  A++F+ D +SIGAFHAVQQGIVVVCSGGN GP P TV+NV
Sbjct: 990  AVESAIHDGVDVLSISLGSSARDFASDTLSIGAFHAVQQGIVVVCSGGNDGPTPGTVTNV 1049

Query: 1034 SPWMFTVAGSFLDRDFVNYVVLGNKKQFKSPSLSSGELPEGKYYPLMNAVEAKAANASDN 1093
            SPWM TVA S +DRDFVNYV LGNK+ FK  SLSSG LP GK+YPL++ V+ KA NA+D 
Sbjct: 1050 SPWMITVAASTVDRDFVNYVALGNKRHFKGVSLSSGGLPRGKFYPLVDGVQVKAGNATDK 1109

Query: 1094 LAQICEYGSLDPTKAKGKIIVCHLGDNSRIDKGFEVVRVGGVGMIIVNDKKNGNNVIVDL 1153
            LA +CE GSLDP KAKGKI++C  GD++R+DK FEV R GG+G+I+VNDK++GN++  D 
Sbjct: 1110 LALLCEDGSLDPAKAKGKIVLCLRGDSARMDKSFEVRRAGGIGLILVNDKEDGNDITADP 1169

Query: 1154 HILPASHLNYEDGLSIAHYMNSTKTPVALITHVRTELGIKPSPVIAGFSSKGPNPITDSM 1213
            H LPASHLNY DG++I  Y+NSTK+P+A ITHV+TELGIKPSP++A FSS+GPNPI DSM
Sbjct: 1170 HFLPASHLNYADGIAIFQYINSTKSPMAFITHVKTELGIKPSPMVADFSSRGPNPIIDSM 1229

Query: 1214 IKPDITAPGGSIIASFTEDVTATKSRFDTRRVPFNVQSGTSMACPHISGVVGLLKTLYPT 1273
            IKPDI APG SI+A+F+E  TAT    DTRRV FN +SGTSMACPHISGVVGLLKTLYP 
Sbjct: 1230 IKPDIAAPGVSILAAFSEYATATDFPLDTRRVSFNFESGTSMACPHISGVVGLLKTLYPK 1289

Query: 1274 WSPAAIKSAIMTTANTRDNTMHPILDSTNVKATPFDYGAGQVNPNNAIDPGLVYDTTIDD 1318
            WSPAAIKSAIMTTA TRDN+M  ILD    KATPF YGAG V+PNNAIDPGLVYDTTI+D
Sbjct: 1290 WSPAAIKSAIMTTAKTRDNSMKSILDYNKAKATPFQYGAGHVHPNNAIDPGLVYDTTIED 1349

BLAST of HG10003277 vs. ExPASy TrEMBL
Match: A0A6J1KUL8 (uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820 PE=3 SV=1)

HSP 1 Score: 1887.5 bits (4888), Expect = 0.0e+00
Identity = 921/1391 (66.21%), Postives = 1095/1391 (78.72%), Query Frame = 0

Query: 14   GVWPESKSFSDAGYGPVPSRWMGVCEGGSDFTCNKKLIGARYFYKGFEAANGAINATLRT 73
            GVWPES SF D+GYGPVPSRWMG CEGGS+F+CN+KLIGARYFY+G+E  NG +N +   
Sbjct: 148  GVWPESHSFHDSGYGPVPSRWMGACEGGSNFSCNRKLIGARYFYRGYEMINGPLNISSLN 207

Query: 74   ARDQEGHGSHTLSTAGGNFVPGANLFGNANGTAKGGSPKARLAAYKVCWPGGLDGGCFDA 133
            ARD EGHG+HTLSTAGGNFV GAN+FGN NGTAKGG+PKAR+AAYKVCWP    G C DA
Sbjct: 208  ARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTAKGGAPKARVAAYKVCWP---QGKCSDA 267

Query: 134  DVLAAFETAISDGVDVLSVSLGSPARDFASDPLAIGAFHAVQHGIVVVCSGGNDGPSPGT 193
            D+LA  E AISDGVDVLS+SLG+ A+DFA DP+++GAFHA+Q GI+VVCS GNDGP PGT
Sbjct: 268  DLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVGAFHAIQQGIIVVCSAGNDGPLPGT 327

Query: 194  ITNVSPWIFTVAASTIDRDFINYVTLGNKKHFKGASLSSGGLPGHKFYPLMNGVQVKAAN 253
            +TNVSPW+FTV AS+IDR F +YV LGNKK  KG+SLSSGGLP  K YPLMN V  KA+N
Sbjct: 328  VTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSSLSSGGLPRGKLYPLMNSVNAKASN 387

Query: 254  ATDNLALLCEDGSLDPTKAKGKIILCLRGDNARMDKGLEVRRAGGVGLILVNGKEDGTSI 313
            A+D LA LCE+GSLDP KA+GKII+CLRGDN RMDK  EV R GGVG+ILVN K  G+ I
Sbjct: 388  ASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGVGMILVNDKISGSDI 447

Query: 314  TADPHLLPASHLNYADGLAIFQYVNSTKAPMALITHVTTELGLKPSPMMGDFSSRGPNPI 373
              D H+LP SH++Y DGL+I +Y+ STK P+A IT V TE+G+KPSP+M  FSSRGPN I
Sbjct: 448  ETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITPVRTEIGIKPSPVMAPFSSRGPNHI 507

Query: 374  TDSMIKPDITAPGVSILASVSESAPATQFPFDTRRVPFNFETGTSMACPHISGVVGLLKT 433
             ++MIKPDI+APGV+I+AS +++  AT  PFD RRVPFN ++GTSM+CPHI+GV GLLK 
Sbjct: 508  AEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRVPFNVQSGTSMSCPHIAGVAGLLKK 567

Query: 434  LYPKWSPAAIKSAIMTTAKTRDNSHKSILDYNKVKATPFSYGAGHVHPNNAMNPGLVYDT 493
            L+P WSPAAIKSAIMTTAKTRDN+  ++LD+NKVKATPF YGAGHVHPN+AM+PGLVYDT
Sbjct: 568  LHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAGHVHPNDAMDPGLVYDT 627

Query: 494  TMEDYMNFLCAQGYNYTALKKFYNKPFVCPKSFATTDLNYPSISVPKLTAGATVTINRRV 553
            T++DY+NFLC QGYN   LKKF NKPFVC K+FA+TDLNYPSISVPKL  G  VT+NRRV
Sbjct: 628  TIDDYLNFLCMQGYNSLTLKKFSNKPFVCAKNFASTDLNYPSISVPKLQIGVPVTVNRRV 687

Query: 554  KNVGTPGTYVASVKGSSKVSVSVEPSTLQFNSVGEEKAFKVVFHYKGQGQGQGHVFGTLI 613
            KNVG+ GTYVA V+    ++V VEPSTLQF+SVGEEKAFK+VFHY  + +  G+VFG L+
Sbjct: 688  KNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEEKAFKLVFHYAQKVRRPGYVFGALV 747

Query: 614  ------------------------------------------------------------ 673
                                                                        
Sbjct: 748  WSDGKHFVRSPIAVNLVNFKAKAMEEYNKISPLLLFFFMLHTAAVPTKNSYIVYLGSHSF 807

Query: 674  -------------------------CKLAAKESIIYSYNRYINGFAAVLDEKEATALEND 733
                                      K+AAK+SI+YSYNRYINGFAAVLDE+EATAL  +
Sbjct: 808  APNPSVYDVQLATESQYDILGSVKGSKVAAKDSILYSYNRYINGFAAVLDEQEATALAKN 867

Query: 734  PSVVSIFENEERELHTTRSWNFLGVENDEGIPPNSIWEAARLGEDTIIANLDTGVWPESK 793
            PSVVS+FEN+ER+LHTTRSW FLGV++D GIP NSIW+AAR G DTII NLDTGVWPES 
Sbjct: 868  PSVVSVFENKERKLHTTRSWGFLGVDSDRGIPQNSIWKAARFGADTIIGNLDTGVWPESP 927

Query: 794  SFNDAGYGPIPSRWRGACVGGANFTCNRKLIGARYFYQGIASAKGIL---NNIFNTTRDH 853
            SFNDAGYGP+PSRWRGAC GG+ F CNRKLIGARYFY+G  +A+G L   N  F++ RDH
Sbjct: 928  SFNDAGYGPVPSRWRGACEGGSKFRCNRKLIGARYFYRGFQAAEGPLTTHNISFDSARDH 987

Query: 854  GGHGSHTLSTAGGNFVPGANIFGYGNGTAKGGSPKARLVAYKVCWPTSSGGCFDADILAA 913
             GHGSHTLSTAGGNFV G N+FG GNGTAKGGSP+AR+VAYKVCWP+ +GGC+D+DILA 
Sbjct: 988  EGHGSHTLSTAGGNFVHGVNVFGNGNGTAKGGSPRARVVAYKVCWPSKNGGCYDSDILAG 1047

Query: 914  FEAAISDGVDVLSVSLGGKAQEFSHDPISIGAFHAVQQGIVVVCSGGNHGPIPRTVSNVS 973
             EAAISDGVDVLS S+G  AQEF++D ISIGAFHAVQ GIVVVCS GN GP P +VSNVS
Sbjct: 1048 IEAAISDGVDVLSASIGTLAQEFANDAISIGAFHAVQHGIVVVCSAGNDGPSPGSVSNVS 1107

Query: 974  PWMFTVAGSFLDRDFVNYVVLGNKKQFKSPSLSSGELPEGKYYPLMNAVEAKAANASDNL 1033
            PWM TV  S +DRDFV+YVVLGNKK+F+  SLSS  LP GK+YPL+ AV+ KAANA+D  
Sbjct: 1108 PWMVTVGASTIDRDFVSYVVLGNKKRFRGSSLSSSRLPAGKFYPLIKAVQVKAANATDGF 1167

Query: 1034 AQICEYGSLDPTKAKGKIIVCHLGDNSRIDKGFEVVRVGGVGMIIVNDKKNGNNVIVDLH 1093
            AQ+C  G+LDPTKAKGKIIVC  G+N+R+ KGFEV RVGGVGM++VN++ +G+ ++ D H
Sbjct: 1168 AQLCMDGTLDPTKAKGKIIVCLRGENARVSKGFEVFRVGGVGMVLVNNQMDGSALVADPH 1227

Query: 1094 ILPASHLNYEDGLSIAHYMNSTKTPVALITHVRTELGIKPSPVIAGFSSKGPNPITDSMI 1153
            ILPASHL+Y DG+SIA Y++STKTPVA ITH  TE+GIKPSP++A FSS+GP+ IT ++I
Sbjct: 1228 ILPASHLSYADGVSIAQYLSSTKTPVASITHASTEMGIKPSPLMASFSSRGPDFITQAVI 1287

Query: 1154 KPDITAPGGSIIASFTEDVTATKSRFDTRRVPFNVQSGTSMACPHISGVVGLLKTLYPTW 1213
            KPDITAPG +IIAS T+D++A+    D RRVPFN++SGTSM+CPHISGV GLLKTL+PTW
Sbjct: 1288 KPDITAPGVNIIASVTDDISASGLPLDKRRVPFNIESGTSMSCPHISGVAGLLKTLHPTW 1347

Query: 1214 SPAAIKSAIMTTANTRDNTMHPILDSTNVKATPFDYGAGQVNPNNAIDPGLVYDTTIDDY 1273
            SPAAIKSA+MTTA TRDNT + +LD T VKATPFDYGAG V+PN+A+DPGLVYDTT+DDY
Sbjct: 1348 SPAAIKSAVMTTAKTRDNTKNTLLDYTKVKATPFDYGAGHVHPNDAMDPGLVYDTTVDDY 1407

Query: 1274 LNFLCARGYNYTQLKKFSNKPFVCAESFAITDLNYPSISVPKLTIDAPMTINRRVKNVGS 1317
            LNFLC RGYN   LKKFSNKPFVCA +FA TD NYPSI VP+L I   +T+NRRVKNVGS
Sbjct: 1408 LNFLCTRGYNSRTLKKFSNKPFVCANNFATTDFNYPSILVPRLQIGGSVTVNRRVKNVGS 1467

BLAST of HG10003277 vs. ExPASy TrEMBL
Match: A0A1S4DX85 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491136 PE=3 SV=1)

HSP 1 Score: 1670.6 bits (4325), Expect = 0.0e+00
Identity = 834/1375 (60.65%), Postives = 1005/1375 (73.09%), Query Frame = 0

Query: 14   GVWPESKSFSDAGYGPVPSRWMGVCEGGSDFTCNKKLIGARYFYKGFEAANGAINATLRT 73
            GVWPESKSF+D GYGPVP+RW G CEGGS F CN+KLIGARYF KG+ A  G++NA+  T
Sbjct: 131  GVWPESKSFNDEGYGPVPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAYAGSLNASYET 190

Query: 74   ARDQEGHGSHTLSTAGGNFVPGANLFGNANGTAKGGSPKARLAAYKVCWPG-GLDGGCFD 133
            ARD EGHG+HTLSTAGGNF+ GAN+FGN NGTAKGGSPKA +AAYKVCWP     GGCFD
Sbjct: 191  ARDNEGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKALVAAYKVCWPQVDSGGGCFD 250

Query: 134  ADVLAAFETAISDGVDVLSVSLGSPARDFASDPLAIGAFHAVQHGIVVVCSGGNDGPSPG 193
            AD+LAA E AISDGVD+LS+SLG  A+DF+ D  AIGAFHAVQ GI+VVCS GN GP+PG
Sbjct: 251  ADILAAIEAAISDGVDILSLSLGGGAKDFSEDVTAIGAFHAVQQGIIVVCSAGNSGPAPG 310

Query: 194  TITNVSPWIFTVAASTIDRDFINYVTLGNKKHFKGASLSSGGLPGHKFYPLMNGVQVKAA 253
            TI N +PWI TV ASTI+RDF +YV LGNKKH KGASLS   LP  KFYPL+N    KA 
Sbjct: 311  TIENGAPWILTVGASTINRDFTSYVALGNKKHIKGASLSDKILPEQKFYPLINAADAKAN 370

Query: 254  NATDNLALLCEDGSLDPTKAKGKIILCLRGDNARMDKGLEVRRAGGVGLILVNGKEDGTS 313
            N + ++A LC+ GSLDP K KGKIILCLRG+NAR+DKG    +AG VG+IL N +++G  
Sbjct: 371  NVSSDVAQLCQAGSLDPKKVKGKIILCLRGENARVDKGYAAAQAGAVGMILANAEQNGDE 430

Query: 314  ITADPHLLPASHLNYADGLAIFQYVNSTKAPMALITHVTTELGLKPSPMMGDFSSRGPNP 373
            + AD HLLP SH++Y DG +I+QY+N TK PMA +THV TELG+KP+P+M  FSSRGPN 
Sbjct: 431  LIADAHLLPVSHVSYTDGQSIYQYINFTKTPMAYMTHVRTELGIKPAPVMASFSSRGPNT 490

Query: 374  ITDSMIKPDITAPGVSILASVSESAPATQFPFDTRRVPFNFETGTSMACPHISGVVGLLK 433
            I +S++KPDITAPGV+ILA+ SE A  +   FD RR+PFN  +GTSM+CPHISG+VGLLK
Sbjct: 491  IEESILKPDITAPGVNILAAYSEDASPSGSLFDNRRIPFNIVSGTSMSCPHISGIVGLLK 550

Query: 434  TLYPKWSPAAIKSAIMTTAKTRDNSHKSILDYNKVKATPFSYGAGHVHPNNAMNPGLVYD 493
            TLYP WSPAAIKSAIMTTA+TR N    IL+   +KA PF+YGAGHV PN AMNPGLVYD
Sbjct: 551  TLYPTWSPAAIKSAIMTTAETRANDLHPILNTVNLKANPFAYGAGHVQPNRAMNPGLVYD 610

Query: 494  TTMEDYMNFLCAQGYNYTALKKFYNKPFVCPKSFATTDLNYPSISVPKLTAGATVTINRR 553
             T  DYMNFLCAQGYN + + KF    FVC KSF  TD NYPSIS+P + +G  VTINRR
Sbjct: 611  LTTNDYMNFLCAQGYNKSQISKFSATSFVCSKSFKLTDFNYPSISIPDMKSG-VVTINRR 670

Query: 554  VKNVGTPGTYVASVKGSSKVSVSVEPSTLQFNSVGEEKAFKVVFHYKGQGQGQGHVFGTL 613
            VKNVG P TYVA VK    VSVSVEP TL+F  + EEK+FKVV       + +G+VFG+L
Sbjct: 671  VKNVGKPSTYVARVKVPQGVSVSVEPRTLKFTGIDEEKSFKVVIGSVANNKHRGYVFGSL 730

Query: 614  I----------------------------------------------------------- 673
            I                                                           
Sbjct: 731  IWEDGKHHVRSPIVVNLGSYIVYLGSHSHGLNPSSIDAQIATESHYNLLGSLLGRYLHSV 790

Query: 674  CKLA------AKESIIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTTRS 733
            C ++      AKE+I YSYNR+INGFAAV+D+K A  L   P VVS+ EN+ R+LHTT S
Sbjct: 791  CCVSKYSNEEAKEAIFYSYNRHINGFAAVVDQKVAEDLAKHPDVVSVLENKGRKLHTTNS 850

Query: 734  WNFLGVENDEGIPPNSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACV 793
            W+FLGVE++  IP NSIW  A  GE TII NLDTGVWPE+KSF+D  YGPIPSRW+G+C 
Sbjct: 851  WSFLGVESNGVIPSNSIWNLASFGESTIIGNLDTGVWPEAKSFDDKEYGPIPSRWKGSCE 910

Query: 794  GGANFTCNRKLIGARYFYQGIASAKGILNNIFNTTRDHGGHGSHTLSTAGGNFVPGANIF 853
            GG+NF CNRKLIGARY+ +G A   G LN+ + + RDH GHG+HTLSTAGGNFVP AN+F
Sbjct: 911  GGSNFQCNRKLIGARYYNKGYAEIAGPLNSSYESARDHEGHGTHTLSTAGGNFVPNANLF 970

Query: 854  GYGNGTAKGGSPKARLVAYKVCWPT--SSGGCFDADILAAFEAAISDGVDVLSVSLGGKA 913
            GYGNGTAKGGSPKA + AYKVCWP     G CFDADILA FEAAI+DGVDVLSVSLGG  
Sbjct: 971  GYGNGTAKGGSPKALVAAYKVCWPKLWLFGECFDADILAGFEAAIADGVDVLSVSLGGDP 1030

Query: 914  QEFSHDPISIGAFHAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVV 973
             +F  D I+IG+FHAVQ GI VVCS GN GP P TVSNV+PW+ TV  S  DR + NYV 
Sbjct: 1031 SDFGQDSIAIGSFHAVQNGITVVCSAGNSGPAPGTVSNVAPWIITVGASTADRLYTNYVA 1090

Query: 974  LGNKKQFKSPSLSSGELPEGKYYPLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIV 1033
            +G+K+ FK  SLS   LP  K+YPL+N+V+AK  N S+  AQ+CE  SLDP K KGKI+V
Sbjct: 1091 IGDKRHFKGASLSEKGLPVQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKGKIVV 1150

Query: 1034 CHLGDNSRIDKGFEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMN 1093
            C  GDN+R +KG+ V + GGVGMI+ N ++NG+++  D H+LPASH+ Y DG  +  Y+N
Sbjct: 1151 CLRGDNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVYQYIN 1210

Query: 1094 STKTPVALITHVRTELGIKPSPVIAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVT 1153
            STK P+A +TH RTE G+KP+P++A FSS+GPN I  S++KPDITAPG +I+A+++ED +
Sbjct: 1211 STKIPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYSEDGS 1270

Query: 1154 ATKSRFDTRRVPFNVQSGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTM 1213
             +   FD RRVPFNV+SGTSM+CPH+SG+VGLLKTLYP WSPAAI+SAIMTTA T+ N +
Sbjct: 1271 PSGLYFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDL 1330

Query: 1214 HPILDSTNVKATPFDYGAGQVNPNNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFSN- 1273
            +PIL +   KA  + YGAG V PN A DPGLVYD + +DYLN+LCA GY   Q+K+FSN 
Sbjct: 1331 NPILSTKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQFSND 1390

Query: 1274 KPFVCAESFAITDLNYPSISVPKLTIDAPMTINRRVKNVGSPGTYVANVEVPEGVVVTVE 1318
              FVC++SF ITDLNYPSIS+P L  D  + I RR+KNVGSPGTYV  V  P GV V+VE
Sbjct: 1391 TSFVCSKSFKITDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVSVSVE 1450

BLAST of HG10003277 vs. ExPASy TrEMBL
Match: A0A6J5V7Q5 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS35141 PE=3 SV=1)

HSP 1 Score: 1585.9 bits (4105), Expect = 0.0e+00
Identity = 802/1360 (58.97%), Postives = 992/1360 (72.94%), Query Frame = 0

Query: 14   GVWPESKSFSDAGYGPVPSRWMGVCE----GGSDFTCNKKLIGARYFYKGFEAANGAINA 73
            GVWPESKSFSD G GP+PS+W G+C+     GS   CN+KLIGARYF KG+ A    +N+
Sbjct: 153  GVWPESKSFSDEGIGPIPSKWRGICQLDTKNGSH--CNRKLIGARYFSKGYLAYASTVNS 212

Query: 74   TLR-----TARDQEGHGSHTLSTAGGNFVPGANLFGNANGTAKGGSPKARLAAYKVCWPG 133
            +        ARD  GHGSHTLSTA GNFVP A++FGN NGTAKGGSPKAR+AAYKVCWP 
Sbjct: 213  SAAKTIQPNARDFGGHGSHTLSTAAGNFVPRASVFGNGNGTAKGGSPKARVAAYKVCWPP 272

Query: 134  GLDGGCFDADVLAAFETAISDGVDVLSVSLGSPARDFASDPLAIGAFHAVQHGIVVVCSG 193
                 CFDAD++AAF+ AISDGVDVLSVSLG  A +F SD +AIG+FHAV+ GI VV S 
Sbjct: 273  INGNECFDADIMAAFDAAISDGVDVLSVSLGGQAAEFFSDGIAIGSFHAVKKGISVVSSA 332

Query: 194  GNDGPSPGTITNVSPWIFTVAASTIDRDFINYVTLGNKKHFKGASLSSGGLPGHKFYPLM 253
            GN GP+PGT++NVSPW+ TV ASTIDR+F +YV LGNKKH KGASLSSG LP   FYPL+
Sbjct: 333  GNSGPTPGTVSNVSPWLLTVGASTIDREFSSYVALGNKKHLKGASLSSGALPSKMFYPLI 392

Query: 254  NGVQVKAANATDNLALLCEDGSLDPTKAKGKIILCLRGDNARMDKGLEVRRAGGVGLILV 313
            + V  KAANA+ + A LC+ GSL+  K +GKI++C+RG+NAR DKG +   AG VG+ILV
Sbjct: 393  SAVDGKAANASSSDAQLCKAGSLEKKKVEGKILVCIRGENARADKGQQAVLAGAVGMILV 452

Query: 314  NGKEDGTSITADPHLLPASHLNYADGLAIFQYVNSTKAPMALITHVTTELGLKPSPMMGD 373
            N K  G  I ADPHLLP SH+NY+DG A+F Y+ STK P+A +T V TE+G KP+P M  
Sbjct: 453  NDKLSGNEIIADPHLLPTSHVNYSDGKAVFAYIKSTKTPVAYLTRVKTEVGAKPAPFMAS 512

Query: 374  FSSRGPNPITDSMIKPDITAPGVSILASVSESAPATQFPFDTRRVPFNFETGTSMACPHI 433
            FSSRGPN I  S++KPDITAPGVSI+A+ + +   T   FD RRV FN E+GTSM+CPH+
Sbjct: 513  FSSRGPNTIEQSILKPDITAPGVSIIAAYTGAEGPTDQKFDKRRVSFNTESGTSMSCPHV 572

Query: 434  SGVVGLLKTLYPKWSPAAIKSAIMTTAKTRDNSHKSILDYNKVKATPFSYGAGHVHPNNA 493
            SG+VGLLKTL+P WSPAAIKSAIMTTA+ RDN+ +++ D +K +ATPF+YGAGHV PN A
Sbjct: 573  SGIVGLLKTLHPSWSPAAIKSAIMTTARKRDNNKEAMQDSSKARATPFAYGAGHVQPNRA 632

Query: 494  MNPGLVYDTTMEDYMNFLCAQGYNYTALKKFYNKPFVCPKSFATTDLNYPSISVPKLTAG 553
            M+PGLVYD T +DY+NFLCA+GYN T LK F N+P  CPK+++  D NYPS++VP L   
Sbjct: 633  MDPGLVYDLTTDDYLNFLCARGYNATLLKVFSNEPHTCPKAYSLADFNYPSVTVPDL-HD 692

Query: 554  ATVTINRRVKNVGTPGTYVASVKGSSKVSVSVEPSTLQFNSVGEEKAFKVVFHYKGQGQG 613
              V + RRVKNVG+PGTYV  +K  + VSVSV+PS++QF ++GEEK FKVV   K QG  
Sbjct: 693  KPVAVTRRVKNVGSPGTYVVHIKEPAGVSVSVKPSSMQFKTIGEEKKFKVVLKPKVQGT- 752

Query: 614  QGHVFGT--------------------------------------------LICKLAAKE 673
            Q +VFG                                             L    +AK+
Sbjct: 753  QDYVFGELNWSDGKHNQSFIVYLGAHSHGPDPSSLDLDSVRKFHYDFLGSFLRSNKSAKD 812

Query: 674  SIIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTTRSWNFLGVENDEGIP 733
             I YSY R+INGFAA+L+E+EA  +   P+V+S+F N+  +L TTRSWNFLG+E +  IP
Sbjct: 813  VIFYSYTRHINGFAAILEEEEAADIAEHPNVISVFLNKGSKLETTRSWNFLGLERNGLIP 872

Query: 734  PNSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACVGGA-NFTCNRKLI 793
             +SIW  ARLGEDTIIAN+DTGVWPESKSF+D G GP+PS+WRG C        CNRKLI
Sbjct: 873  SHSIWMKARLGEDTIIANIDTGVWPESKSFSDEGLGPVPSKWRGICQHDTKRVRCNRKLI 932

Query: 794  GARYFYQGIASAKGILNNIFNTTRDHGGHGSHTLSTAGGNFVPGANIFGYGNGTAKGGSP 853
            G RYF  G+A   G LN+ F+T RD+ GHGSHTL+TA GNFVPG ++FG GNGTAKGGSP
Sbjct: 933  GTRYFNNGLAMYAGPLNSSFSTARDYDGHGSHTLATAAGNFVPGVSVFGNGNGTAKGGSP 992

Query: 854  KARLVAYKVCWPTSSG-GCFDADILAAFEAAISDGVDVLSVSLGGKAQEFSHDPISIGAF 913
            +A + AYKVCWP   G  CFDAD+LAAF+AAISDGVD++SVSLGG AQEF    ISIGAF
Sbjct: 993  RAHVAAYKVCWPPYEGVQCFDADVLAAFDAAISDGVDIISVSLGGGAQEFFKSSISIGAF 1052

Query: 914  HAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVVLGNKKQFKSPSLS 973
            HAV+ GIVVV + GN GP P TV N+SPW+ TV    +DR+F +YV LGNKK  K  SLS
Sbjct: 1053 HAVKHGIVVVSAAGNTGPNPGTVLNLSPWLLTVGAGTIDREFTSYVSLGNKKHLKGVSLS 1112

Query: 974  SGELPEGKYYPLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIVC--HLGDNSRIDK 1033
            +  LP  K+YPL++A EAK ANAS   A IC+ G+LDP K KGKI+VC     DN+R +K
Sbjct: 1113 AKGLPSEKFYPLVSAAEAKHANASTAEAIICQGGTLDPRKVKGKILVCLREYNDNARTEK 1172

Query: 1034 GFEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMNSTKTPVALITH 1093
             ++    G VGMI+VND+++GN+V+ D H+L  SH+NY DG  I  Y+ STKTP+A +T 
Sbjct: 1173 SWQADMAGAVGMILVNDEQSGNDVVADPHVLLVSHVNYTDGKYIFDYIKSTKTPMAYLTR 1232

Query: 1094 VRTELGIKPSPVIAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVTATKSRFDTRRV 1153
            V+TELG KP+P +A FSS+GPN     ++KPDI APG SIIA++TE    T    DTRRV
Sbjct: 1233 VKTELGSKPAPFVATFSSRGPNLQEQGILKPDIIAPGVSIIAAYTEAAGPTSQISDTRRV 1292

Query: 1154 PFNVQSGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTMHPIL-DSTNVK 1213
            PFNVQ+G+SMACPH SG+ GLL+TL+P WSPAAIKSAIMTTA T+D+ M PIL DS+ VK
Sbjct: 1293 PFNVQTGSSMACPHASGIAGLLRTLHPDWSPAAIKSAIMTTATTQDDHMEPILDDSSYVK 1352

Query: 1214 ATPFDYGAGQVNPNNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFSNKPFVCAESFAI 1273
            ATPF YG+G + PN A+DPGLVY+ T  DYLNFLCARGYN T +K FSN  + C++SF++
Sbjct: 1353 ATPFAYGSGHIQPNKAMDPGLVYNLTTLDYLNFLCARGYNETMIKSFSNSTYKCSKSFSL 1412

Query: 1274 TDLNYPSISVPKLTIDAPMTINRRVKNVGSPGTYVANVEVPEGVVVTVEPNTLQFNNVGE 1316
             D NYPSISVP L+ D+ +TINR+V NVGSPGTY  +V+ P  V V VEP  L+F  +GE
Sbjct: 1413 ADFNYPSISVPNLSEDS-VTINRKVTNVGSPGTYKVHVKEPSEVEVLVEPRRLKFKRIGE 1472

BLAST of HG10003277 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 815.8 bits (2106), Expect = 4.9e-236
Identity = 401/703 (57.04%), Postives = 511/703 (72.69%), Query Frame = 0

Query: 618  AKESIIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTTRSWNFLGVENDE 677
            A ++I YSY ++INGFAA LD   A  +   P VVS+F N+  +LHTTRSW+FLG+E++ 
Sbjct: 71   ATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNS 130

Query: 678  GIPPNSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACVG--GANFTCN 737
             +P +SIW  AR GEDTIIANLDTGVWPESKSF D G GPIPSRW+G C     A F CN
Sbjct: 131  YVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCN 190

Query: 738  RKLIGARYFYQGIASAKGILNNIFNTTRDHGGHGSHTLSTAGGNFVPGANIFGYGNGTAK 797
            RKLIGARYF +G A+A G LN+ F++ RD  GHGSHTLSTA G+FVPG +IFG GNGTAK
Sbjct: 191  RKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAK 250

Query: 798  GGSPKARLVAYKVCWPTSSGG-CFDADILAAFEAAISDGVDVLSVSLGGKAQEFSHDPIS 857
            GGSP+AR+ AYKVCWP   G  C+DAD+LAAF+AAI DG DV+SVSLGG+   F +D ++
Sbjct: 251  GGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVA 310

Query: 858  IGAFHAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVVLGNKKQFKS 917
            IG+FHA ++ IVVVCS GN GP   TVSNV+PW  TV  S +DR+F + +VLGN K +K 
Sbjct: 311  IGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKG 370

Query: 918  PSLSSGELPEGKYYPLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIVCHLGDNSRI 977
             SLSS  LP  K+YP+M +V AKA NAS   AQ+C+ GSLDP K KGKI+VC  G N R+
Sbjct: 371  QSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRV 430

Query: 978  DKGFEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMNSTKTPVALI 1037
            +KG  V   GG+GM++ N    GN+++ D H+LPA+ L  +D  +++ Y++ TK P+A I
Sbjct: 431  EKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHI 490

Query: 1038 THVRTELGIKPSPVIAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVTATKSRFDTR 1097
            T  RT+LG+KP+PV+A FSSKGP+ +   ++KPDITAPG S+IA++T  V+ T  +FD R
Sbjct: 491  TPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPR 550

Query: 1098 RVPFNVQSGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTMHPILDSTNV 1157
            R+ FN  SGTSM+CPHISG+ GLLKT YP+WSPAAI+SAIMTTA   D+   PI ++TN+
Sbjct: 551  RLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNM 610

Query: 1158 KATPFDYGAGQVNPNNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFSNKPFVCAE-SF 1217
            KATPF +GAG V PN A++PGLVYD  I DYLNFLC+ GYN +Q+  FS   F C+    
Sbjct: 611  KATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKI 670

Query: 1218 AITDLNYPSISVPKLTIDAPMTINRRVKNVGSPGTYVANVEVPEGVVVTVEPNTLQFNNV 1277
            ++ +LNYPSI+VP LT  + +T++R VKNVG P  Y   V  P+GV V V+P +L F  V
Sbjct: 671  SLVNLNYPSITVPNLT-SSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKV 730

Query: 1278 GEEKAFKVVFRYIGEGQREGYMFGTLIWSDGKHFVRSPIAMKL 1317
            GE+K FKV+         +GY+FG L+WSD KH VRSPI +KL
Sbjct: 731  GEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of HG10003277 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 782.3 bits (2019), Expect = 6.0e-226
Identity = 393/702 (55.98%), Postives = 491/702 (69.94%), Query Frame = 0

Query: 618  AKESIIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTTRSWNFLGVENDE 677
            AKE+I YSY R+INGFAA+LDE EA  +   P VVS+F N+ R+LHTT SWNF+ +  + 
Sbjct: 81   AKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNG 140

Query: 678  GIPPNSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACVGGANFTCNRK 737
             +  +S+W  A  GEDTIIANLDTGVWPESKSF+D GYG +P+RW+G C    +  CNRK
Sbjct: 141  VVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRC--HKDVPCNRK 200

Query: 738  LIGARYFYQGIASAKGILNNI-FNTTRDHGGHGSHTLSTAGGNFVPGANIFGYGNGTAKG 797
            LIGARYF +G  +  G+ +N  + T RDH GHGSHTLSTA GNFVPGAN+FG GNGTA G
Sbjct: 201  LIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASG 260

Query: 798  GSPKARLVAYKVCWPTSSGG-CFDADILAAFEAAISDGVDVLSVSLGGKAQEFSHDPISI 857
            GSPKAR+ AYKVCWP   G  CFDADILAA EAAI DGVDVLS S+GG A ++  D I+I
Sbjct: 261  GSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAI 320

Query: 858  GAFHAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVVLGNKKQFKSP 917
            G+FHAV+ G+ VVCS GN GP   TVSNV+PW+ TV  S +DR+F  +V L N + FK  
Sbjct: 321  GSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGT 380

Query: 918  SLSSGELPEGKYYPLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIVCHLGDNSRID 977
            SLS   LPE K Y L++A +A  AN +   A +C+ GSLDP K KGKI+VC  GDN+R+D
Sbjct: 381  SLSK-PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVD 440

Query: 978  KGFEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMNSTKTPVALIT 1037
            KG +    G  GM++ NDK +GN +I D H+LPAS ++Y+DG ++  Y++STK P   I 
Sbjct: 441  KGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIK 500

Query: 1038 HVRTELGIKPSPVIAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVTATKSRFDTRR 1097
                 L  KP+P +A FSS+GPN IT  ++KPDITAPG +IIA+FTE    T    D RR
Sbjct: 501  APTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRR 560

Query: 1098 VPFNVQSGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTMHPILDSTNVK 1157
             PFN +SGTSM+CPHISGVVGLLKTL+P WSPAAI+SAIMTT+ TR+N   P++D +  K
Sbjct: 561  TPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKK 620

Query: 1158 ATPFDYGAGQVNPNNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFSNKP-FVCAESFA 1217
            A PF YG+G V PN A  PGLVYD T  DYL+FLCA GYN T ++ F+  P + C +   
Sbjct: 621  ANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGAN 680

Query: 1218 ITDLNYPSISVPKLTIDAPMTINRRVKNVGSPGTYVANVEVPEGVVVTVEPNTLQFNNVG 1277
            + D NYPSI+VP LT    +T+ R++KNVG P TY A    P GV V+VEP  L FN  G
Sbjct: 681  LLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTG 740

Query: 1278 EEKAFKVVFRYIGEGQREGYMFGTLIWSDGKHFVRSPIAMKL 1317
            E K F++  R +      GY+FG L W+D  H+VRSPI ++L
Sbjct: 741  EVKIFQMTLRPL-PVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of HG10003277 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 601.7 bits (1550), Expect = 1.4e-171
Identity = 317/702 (45.16%), Postives = 442/702 (62.96%), Query Frame = 0

Query: 622  IIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTTRSWNFLGVENDEGIPP 681
            ++Y+Y   I+GF+  L ++EA +L   P V+S+      ELHTTR+  FLG++       
Sbjct: 65   LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA--- 124

Query: 682  NSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACVGGANFT---CNRKL 741
              ++  A    D ++  LDTGVWPESKS++D G+GPIPS W+G C  G NFT   CNRKL
Sbjct: 125  -DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 184

Query: 742  IGARYFYQGIASAKGILNNI--FNTTRDHGGHGSHTLSTAGGNFVPGANIFGYGNGTAKG 801
            IGAR+F +G  S  G ++      + RD  GHG+HT STA G+ V GA++ GY +GTA+G
Sbjct: 185  IGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARG 244

Query: 802  GSPKARLVAYKVCWPTSSGGCFDADILAAFEAAISDGVDVLSVSLGGKAQEFSHDPISIG 861
             +P+AR+  YKVCW    GGCF +DILAA + AI+D V+VLS+SLGG   ++  D ++IG
Sbjct: 245  MAPRARVAVYKVCW---LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIG 304

Query: 862  AFHAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVVLGNKKQFKSPS 921
            AF A+++GI+V CS GN GP   ++SNV+PW+ TV    LDRDF    +LGN K F   S
Sbjct: 305  AFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVS 364

Query: 922  LSSGELPEGKYYPLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIVCHLGDNSRIDK 981
            L  GE    K  P + A    A+NA++    +C  G+L P K KGKI++C  G N+R+ K
Sbjct: 365  LFKGEALPDKLLPFIYA--GNASNATN--GNLCMTGTLIPEKVKGKIVMCDRGINARVQK 424

Query: 982  GFEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMNSTKTPVALITH 1041
            G  V   GGVGMI+ N   NG  ++ D H+LPA+ +  + G  I HY+ +   P A I+ 
Sbjct: 425  GDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISI 484

Query: 1042 VRTELGIKPSPVIAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVTATKSRFDTRRV 1101
            + T +G+KPSPV+A FSS+GPN IT +++KPD+ APG +I+A++T     T    D+RRV
Sbjct: 485  LGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 544

Query: 1102 PFNVQSGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTMHPILD-STNVK 1161
             FN+ SGTSM+CPH+SG+  LLK+++P WSPAAI+SA+MTTA        P+LD +T   
Sbjct: 545  EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKP 604

Query: 1162 ATPFDYGAGQVNPNNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFSNKPFVC--AESF 1221
            +TPFD+GAG V+P  A +PGL+YD T +DYL FLCA  Y   Q++  S + + C  ++S+
Sbjct: 605  STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 664

Query: 1222 AITDLNYPSISVPKLTIDAPMTINRRVKNVGSPGTYVANV-EVPEGVVVTVEPNTLQFNN 1281
            ++ DLNYPS +V    + A     R V +VG  GTY   V     GV ++VEP  L F  
Sbjct: 665  SVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKE 724

Query: 1282 VGEEKAFKVVFRYIGEGQREGYMFGTLIWSDGKHFVRSPIAM 1315
              E+K++ V F            FG++ WSDGKH V SP+A+
Sbjct: 725  ANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of HG10003277 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 563.5 bits (1451), Expect = 4.4e-160
Identity = 309/721 (42.86%), Postives = 445/721 (61.72%), Query Frame = 0

Query: 615  KLAAKESIIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTTRSWNFLGVE 674
            + A +  I++ Y+   +GF+AV+   EA  L N P+V+++FE+  RELHTTRS  FLG++
Sbjct: 51   EFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQ 110

Query: 675  NDEGIPPNSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACVGGANFT- 734
            N +G     +W  +  G D II   DTG+WPE +SF+D   GPIP RWRG C  GA F+ 
Sbjct: 111  NQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSP 170

Query: 735  --CNRKLIGARYFYQGIASA--KGILNNI-FNTTRDHGGHGSHTLSTAGGNFVPGANIFG 794
              CNRK+IGAR+F +G  +A   GI   + F + RD  GHG+HT STA G     A++ G
Sbjct: 171  RNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSG 230

Query: 795  YGNGTAKGGSPKARLVAYKVCWPTSSGGCFDADILAAFEAAISDGVDVLSVSLGGK---A 854
            Y +G AKG +PKAR+ AYKVCW  S  GC D+DILAAF+AA+ DGVDV+S+S+GG     
Sbjct: 231  YASGVAKGVAPKARIAAYKVCWKDS--GCLDSDILAAFDAAVRDGVDVISISIGGGDGIT 290

Query: 855  QEFSHDPISIGAFHAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVV 914
              +  DPI+IG++ A  +GI V  S GN GP   +V+N++PW+ TV  S +DR+F    +
Sbjct: 291  SPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAI 350

Query: 915  LGNKKQFKSPSLSSGELPEGKYYPLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIV 974
            LG+  + +  SL +G    G+ +P++   ++  ++AS     +C   +LDP + +GKI++
Sbjct: 351  LGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLDPKQVRGKIVI 410

Query: 975  CHLGDNSRIDKGFEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMN 1034
            C  G + R+ KG  V + GGVGMI+ N   NG  ++ D H++PA  +   +G  I  Y +
Sbjct: 411  CDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYAS 470

Query: 1035 STKTPVALITHVRTELGIKPSPVIAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVT 1094
            S   P+A I    T +GIKP+PVIA FS +GPN ++  ++KPD+ APG +I+A++T+ V 
Sbjct: 471  SHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVG 530

Query: 1095 ATKSRFDTRRVPFNVQSGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTM 1154
             T    D R+  FN+ SGTSMACPH+SG   LLK+ +P WSPA I+SA+MTT N  DN+ 
Sbjct: 531  PTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSN 590

Query: 1155 HPILD-STNVKATPFDYGAGQVNPNNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFSN 1214
              ++D ST   ATP+DYG+G +N   A++PGLVYD T DDY+ FLC+ GY    ++  + 
Sbjct: 591  RSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITR 650

Query: 1215 KPFVCAESFAIT--DLNYPSISVPKLTIDAPM---TINRRVKNVG-SPGTYVANVEVPEG 1274
             P  C  +   +  +LNYPSI+    T    +   T+ R   NVG +   Y A +E P G
Sbjct: 651  TPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRG 710

Query: 1275 VVVTVEPNTLQFNNVGEEKAFKVVF----RYIGEGQREGYMFGTLIWSD-GKHFVRSPIA 1315
            V VTV+P  L F +  + +++ V      R +  G+  G +FG++ W D GKH VRSPI 
Sbjct: 711  VTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGE-TGAVFGSVTWFDGGKHVVRSPIV 758

BLAST of HG10003277 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 563.5 bits (1451), Expect = 4.4e-160
Identity = 305/705 (43.26%), Postives = 428/705 (60.71%), Query Frame = 0

Query: 622  IIYSYNRYINGFAAVLDEKEATALENDPSVVSIFENEERELHTTRSWNFLGVENDEGIPP 681
            I+Y+Y    +G AA L ++EA  LE +  VV++      ELHTTRS  FLG+E  E    
Sbjct: 79   ILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQES--- 138

Query: 682  NSIWEAARLGEDTIIANLDTGVWPESKSFNDAGYGPIPSRWRGACVGGANF---TCNRKL 741
              +W       D ++  LDTG+WPES+SFND G  P+P+ WRGAC  G  F    CNRK+
Sbjct: 139  ERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKI 198

Query: 742  IGARYFYQGIASAKGILNN--IFNTTRDHGGHGSHTLSTAGGNFVPGANIFGYGNGTAKG 801
            +GAR FY+G  +A G ++    + + RD  GHG+HT +T  G+ V GAN+FG+  GTA+G
Sbjct: 199  VGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARG 258

Query: 802  GSPKARLVAYKVCWPTSSGGCFDADILAAFEAAISDGVDVLSVSLGGKAQEFSHDPISIG 861
             + KAR+ AYKVCW    GGCF +DIL+A + A++DGV VLS+SLGG    +S D +SI 
Sbjct: 259  MAQKARVAAYKVCW---VGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIA 318

Query: 862  AFHAVQQGIVVVCSGGNHGPIPRTVSNVSPWMFTVAGSFLDRDFVNYVVLGNKKQFKSPS 921
             F A++ G+ V CS GN GP P +++NVSPW+ TV  S +DRDF   V +G  + FK  S
Sbjct: 319  TFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVS 378

Query: 922  LSSGE--LPEGKYYPLMNAVEAKAANASDNLAQICEYGSLDPTKAKGKIIVCHLGDNSRI 981
            L  G   LP+ K YPL   V      +S +    C  G+LD     GKI++C  G   R+
Sbjct: 379  LYKGRTVLPKNKQYPL---VYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRV 438

Query: 982  DKGFEVVRVGGVGMIIVNDKKNGNNVIVDLHILPASHLNYEDGLSIAHYMNSTKTPVALI 1041
             KG  V R GG+GM++ N   NG  ++ D H+LPA  +  ++G  I  Y  ++K   A +
Sbjct: 439  QKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASL 498

Query: 1042 THVRTELGIKPSPVIAGFSSKGPNPITDSMIKPDITAPGGSIIASFTEDVTATKSRFDTR 1101
              + T +GIKPSPV+A FSS+GPN ++  ++KPD+ APG +I+A++T D+  +    D R
Sbjct: 499  EILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPR 558

Query: 1102 RVPFNVQSGTSMACPHISGVVGLLKTLYPTWSPAAIKSAIMTTANTRDNTMHPILDSTN- 1161
            RV FN+ SGTSM+CPH+SGV  L+K+ +P WSPAAIKSA+MTTA   DN   P+ D++  
Sbjct: 559  RVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGA 618

Query: 1162 VKATPFDYGAGQVNPNNAIDPGLVYDTTIDDYLNFLCARGYNYTQLKKFS-NKPFVCAES 1221
              ++P+D+GAG ++P  A DPGLVYD    +Y  FLC +  + +QLK F+ +    C  +
Sbjct: 619  APSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHT 678

Query: 1222 FAIT--DLNYPSISV--PKLTIDAPMTINRRVKNVGSP-GTYVANVEVPEGVVVTVEPNT 1281
             A    +LNYP+IS   P+ T    MT+ R V NVG    +Y  +V   +G  VTV+P T
Sbjct: 679  LAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKT 738

Query: 1282 LQFNNVGEEKAFKVVFRYIGEGQREGYMFGTLIWSDGKHFVRSPI 1313
            L F +  ++ ++ V FR     +R    FG L+W    H VRSP+
Sbjct: 739  LNFTSKHQKLSYTVTFRTRFRMKRP--EFGGLVWKSTTHKVRSPV 772