HG10003182 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10003182
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionE3 ubiquitin-protein ligase RNF181-like protein
LocationChr11: 18440653 .. 18441363 (-)
RNA-Seq ExpressionHG10003182
SyntenyHG10003182
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTTCGCAACTGCTTCCACCTTCCGCTACAGTATCCGAAGCAATGACGGCGCCGACCAATCTCTCCAAATCAGATTAGGATTTTTGTGGCGTGTACTCTCCCGATCACCGTCTGCCGGTCTTCCGTACGAAACCCTCTACGAAATTCCTTTAAGTTCAATGCGGGAGACTACATTCTCTCTCTATCTGCGAGAGCTCGAAGATCCGCTGCTCTGCGAAGCTTACATCGCTCAATTGCTGTCCTCTTTGGATCTCGGTGCCGTAACCTGTGAAATAATTTCTCGGAAGATCACATCCGTCGTCATTGAAGTATTGGAATCCGGAAACTACGATACGAAGTTCCAGATCGCAGTGGAAATCGATCTCGTCCAGATGTTCTGGGCAGAGGAGGAGCAGAGTTCGGTTCCGGTGGAGAATGGTGAAAGGTGCGGTGTTGTGGTGGAGGAGAGGGTGGTTCTGGAGGAAGCTCCGGCGAGGAGAAGGCGGGCGGAGAGGGAGAGTAAGAGGTTGAAGATGGAGAAATTTGATGGGTTTGAGAGAGGAGAGTTTGGAGATTGTTGTGTTTGTCACGAAGAATTGAATGATGTGAGGAAGGAGGTGAGTACAATCCAGTGTGGTCATGTCTATCATAAATCATGTATTCTCACTTGGGTTCAAAGGAGCAACTCTTGTCCCTTGTGTAGAAGGGAAATTAAATTTGAAATTTAA

mRNA sequence

ATGTCTTTCGCAACTGCTTCCACCTTCCGCTACAGTATCCGAAGCAATGACGGCGCCGACCAATCTCTCCAAATCAGATTAGGATTTTTGTGGCGTGTACTCTCCCGATCACCGTCTGCCGGTCTTCCGTACGAAACCCTCTACGAAATTCCTTTAAGTTCAATGCGGGAGACTACATTCTCTCTCTATCTGCGAGAGCTCGAAGATCCGCTGCTCTGCGAAGCTTACATCGCTCAATTGCTGTCCTCTTTGGATCTCGGTGCCGTAACCTGTGAAATAATTTCTCGGAAGATCACATCCGTCGTCATTGAAGTATTGGAATCCGGAAACTACGATACGAAGTTCCAGATCGCAGTGGAAATCGATCTCGTCCAGATGTTCTGGGCAGAGGAGGAGCAGAGTTCGGTTCCGGTGGAGAATGGTGAAAGGTGCGGTGTTGTGGTGGAGGAGAGGGTGGTTCTGGAGGAAGCTCCGGCGAGGAGAAGGCGGGCGGAGAGGGAGAGTAAGAGGTTGAAGATGGAGAAATTTGATGGGTTTGAGAGAGGAGAGTTTGGAGATTGTTGTGTTTGTCACGAAGAATTGAATGATGTGAGGAAGGAGGTGAGTACAATCCAGTGTGGTCATGTCTATCATAAATCATGTATTCTCACTTGGGTTCAAAGGAGCAACTCTTGTCCCTTGTGTAGAAGGGAAATTAAATTTGAAATTTAA

Coding sequence (CDS)

ATGTCTTTCGCAACTGCTTCCACCTTCCGCTACAGTATCCGAAGCAATGACGGCGCCGACCAATCTCTCCAAATCAGATTAGGATTTTTGTGGCGTGTACTCTCCCGATCACCGTCTGCCGGTCTTCCGTACGAAACCCTCTACGAAATTCCTTTAAGTTCAATGCGGGAGACTACATTCTCTCTCTATCTGCGAGAGCTCGAAGATCCGCTGCTCTGCGAAGCTTACATCGCTCAATTGCTGTCCTCTTTGGATCTCGGTGCCGTAACCTGTGAAATAATTTCTCGGAAGATCACATCCGTCGTCATTGAAGTATTGGAATCCGGAAACTACGATACGAAGTTCCAGATCGCAGTGGAAATCGATCTCGTCCAGATGTTCTGGGCAGAGGAGGAGCAGAGTTCGGTTCCGGTGGAGAATGGTGAAAGGTGCGGTGTTGTGGTGGAGGAGAGGGTGGTTCTGGAGGAAGCTCCGGCGAGGAGAAGGCGGGCGGAGAGGGAGAGTAAGAGGTTGAAGATGGAGAAATTTGATGGGTTTGAGAGAGGAGAGTTTGGAGATTGTTGTGTTTGTCACGAAGAATTGAATGATGTGAGGAAGGAGGTGAGTACAATCCAGTGTGGTCATGTCTATCATAAATCATGTATTCTCACTTGGGTTCAAAGGAGCAACTCTTGTCCCTTGTGTAGAAGGGAAATTAAATTTGAAATTTAA

Protein sequence

MSFATASTFRYSIRSNDGADQSLQIRLGFLWRVLSRSPSAGLPYETLYEIPLSSMRETTFSLYLRELEDPLLCEAYIAQLLSSLDLGAVTCEIISRKITSVVIEVLESGNYDTKFQIAVEIDLVQMFWAEEEQSSVPVENGERCGVVVEERVVLEEAPARRRRAERESKRLKMEKFDGFERGEFGDCCVCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREIKFEI
Homology
BLAST of HG10003182 vs. NCBI nr
Match: XP_038876865.1 (putative RING-H2 finger protein ATL53 [Benincasa hispida])

HSP 1 Score: 393.7 bits (1010), Expect = 1.2e-105
Identity = 195/236 (82.63%), Postives = 211/236 (89.41%), Query Frame = 0

Query: 1   MSFATASTFRYSIRSNDGADQSLQIRLGFLWRVLSRSPSAGLPYETLYEIPLSSMRETTF 60
           MS  TASTFRYS+R NDG DQSL IRLGFLWR+LSRSPS+GLPYE LYEIP SS RETTF
Sbjct: 1   MSLETASTFRYSLRRNDGVDQSLHIRLGFLWRLLSRSPSSGLPYEILYEIPSSSPRETTF 60

Query: 61  SLYLRELEDPLLCEAYIAQLLSSLDLGAVTCEIISRKITSVVIEVLESGNYDTKFQIAVE 120
            L LR+LEDPL CEAYIAQLL SLDLGAVTC+I++RKITSVV+EVLESGNYD KFQIA E
Sbjct: 61  PLSLRDLEDPLFCEAYIAQLLHSLDLGAVTCDIVARKITSVVVEVLESGNYDVKFQIAAE 120

Query: 121 IDLVQMFWAEEEQSSVPVENGERCGVVVEERVVLEEAPARRRRAERESKRLKMEKFDGFE 180
           ID VQMFWAEEE+SSVPVENGER G+VVEER VLEEAPARRR AERESKRLKMEKFD  +
Sbjct: 121 IDHVQMFWAEEERSSVPVENGERRGIVVEERAVLEEAPARRRAAERESKRLKMEKFDELK 180

Query: 181 RGEFGDCCVCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREIKFEI 237
           R E GDCCVC+EELNDVRKEVS I+CGH+YHKSCILTW+Q  NSCPLCRREI+FEI
Sbjct: 181 RAEMGDCCVCYEELNDVRKEVSRIRCGHIYHKSCILTWLQSRNSCPLCRREIEFEI 236

BLAST of HG10003182 vs. NCBI nr
Match: KAE8650076.1 (hypothetical protein Csa_011336 [Cucumis sativus])

HSP 1 Score: 272.3 bits (695), Expect = 3.9e-69
Identity = 153/241 (63.49%), Postives = 175/241 (72.61%), Query Frame = 0

Query: 1   MSFATASTFRYSIRSNDGADQSLQIRLGFLWRVLSRSPSAGLPYETLYEIPLSSMRETTF 60
           M   +ASTFRYSIR   G +QSLQIRLG + R+LS SPSAGLPYE L E     + E TF
Sbjct: 1   MPLESASTFRYSIRRRCGVEQSLQIRLGRIVRLLSGSPSAGLPYEILQESASIPVHEATF 60

Query: 61  SLYLRELEDPLLCEAYIAQLLSSLDLGAVTCEIISRKITSVVIEVLESGNYDTKFQIAVE 120
            L LR++ED  LCE YI QLLSS +LG VT  IIS+KITS+V+EVL +G+YD KF++  E
Sbjct: 61  PLPLRDVEDSCLCEIYILQLLSSFNLGTVTSGIISKKITSMVVEVLGAGDYDAKFEMVAE 120

Query: 121 IDLVQM-FWAEEEQSS--VPVENGERCGVVVEERVVLEEAPARRRRAERESKR-LKMEKF 180
           ID ++M FW EEE+ S      N ER GVVVEERVVLE  P RRR  ERE +R  + EK 
Sbjct: 121 IDHIRMYFWTEEEERSWVGRGNNSERRGVVVEERVVLEGVPGRRRAGEREREREREREKK 180

Query: 181 DGF-ERGEFGDCCVCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREIKFE 237
            GF ER E GDC VC EEL DVRKEVS I+CGHVYHKSCILTWVQ  NSCPLCRRE+ FE
Sbjct: 181 GGFDERRELGDCSVCQEELKDVRKEVSRIRCGHVYHKSCILTWVQMRNSCPLCRREVVFE 240

BLAST of HG10003182 vs. NCBI nr
Match: XP_038905732.1 (E3 ubiquitin-protein ligase arkadia-A-like [Benincasa hispida])

HSP 1 Score: 261.2 bits (666), Expect = 8.9e-66
Identity = 138/233 (59.23%), Postives = 167/233 (71.67%), Query Frame = 0

Query: 2   SFATASTFRYSIRSNDGA-DQSLQIRLGFLWRVLSRSPSAGLPYETLYEIPLSSMRETTF 61
           S  TASTFR+S+R +D + DQSL IRLGF+ R LSRSPSAGLPYE L+E     MR   F
Sbjct: 3   SLQTASTFRFSLRRHDDSDDQSLLIRLGFMHRQLSRSPSAGLPYEILHESNSIPMRINMF 62

Query: 62  SLYLRELEDPLLCEAYIAQLLSSLDLGAVTCEIISRKITSVVIEVLESGNYDTKFQIAVE 121
            L LR+LEDPLLC  Y+ +LLS LDL AV C+II+  ITS + EVL +GN++ KF+I  +
Sbjct: 63  PLPLRDLEDPLLCRVYVLRLLSYLDLNAVACDIIAHDITSFICEVLAAGNFEVKFRIVAD 122

Query: 122 IDLVQMFWAEEEQSSVPVENGERCGVVVEERVVLEEAPARRRRAERESKRLKMEKFDGFE 181
           ID ++MFW E+   S       R GVV EE V+LEEAP RR  A+   +RLK EKFDGF 
Sbjct: 123 IDHIRMFWREQIGES-------RRGVVAEETVILEEAPTRRTAAKTAIERLKREKFDGFR 182

Query: 182 RGEFGDCCVCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREIK 234
             E GDCCVC EEL + +KEVS I CGHVYHKSCILTW Q ++SCPLCRR+I+
Sbjct: 183 GEELGDCCVCCEELKEGKKEVSRIGCGHVYHKSCILTWAQNNDSCPLCRRKIE 228

BLAST of HG10003182 vs. NCBI nr
Match: XP_023539480.1 (E3 ubiquitin-protein ligase RNF165-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 208.0 bits (528), Expect = 9.0e-50
Identity = 107/233 (45.92%), Postives = 150/233 (64.38%), Query Frame = 0

Query: 1   MSFATASTFRYSIRSNDGADQSLQIRLGFLWRVLSRSPSAGLPYETLYEIPLSSMRETTF 60
           MS A  +TFR ++   DG DQ LQIR+G   R+L+ SP +G P+  ++E   ++M +  F
Sbjct: 1   MSLADLATFRCNLHRYDGPDQLLQIRIGHSSRLLTGSPFSGYPFNIVHETLPTTMADAQF 60

Query: 61  SLYLRELEDPLLCEAYIAQLLSSLDLGAVTCEIISRKITSVVIEVLESGNYDTKFQIAVE 120
            L LRELEDPL C+AYI + LSS +LGA     I+R+I S V++ +  GNY+  F I  E
Sbjct: 61  PLPLRELEDPLFCQAYIKEFLSSYNLGATIKNAIARRIASFVVQ-MACGNYEMTFYIVAE 120

Query: 121 IDLVQMFWAEEEQSSVPVENGERCGVVVEERVVLEEAPARRRRAERESKRLKMEKFDGFE 180
           ID +QMFW ++  ++   E  E      EE  + ++   RR   E   +RLK EKFDGF 
Sbjct: 121 IDYIQMFWMDDSATAAEEEEDEE-----EETEIFDDFSGRRGAPETAIERLKKEKFDGFG 180

Query: 181 RGEFGDCCVCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREIK 234
             + G+C VC++E+ D  +EVS I CGHVYHKSC+LTW+QR+NSCPLCR +++
Sbjct: 181 GEDLGNCSVCYDEMKDGGEEVSRIPCGHVYHKSCVLTWLQRNNSCPLCRNKLE 227

BLAST of HG10003182 vs. NCBI nr
Match: KAG6605180.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 206.5 bits (524), Expect = 2.6e-49
Identity = 112/232 (48.28%), Postives = 151/232 (65.09%), Query Frame = 0

Query: 1   MSFATASTFRYSIRSNDGADQSLQIRLGFLWRVLSRSPSAGLPYETLYEIPLSSMRETTF 60
           M FA +ST RYS+R  DG DQ LQ+RLG+  R++SRSP +GLPY+ L EIP + + +T F
Sbjct: 1   MLFANSSTLRYSLRRKDGTDQLLQVRLGYSSRLISRSPYSGLPYDILEEIPSTPIADTLF 60

Query: 61  SLYLRELEDPLLCEAYIAQLLSSLDLGAVTCEIISRKITSVVIEVLESGNYDTKFQIAVE 120
            L LRELEDPL C AY+ +L+SSL+L +++C  I++KITS+V +   + +YD KFQ+  +
Sbjct: 61  PLPLRELEDPLFCHAYLVELVSSLNLDSISCNPIAQKITSLVTQ-WAAVDYDAKFQLVAD 120

Query: 121 IDLVQMFWAEEEQSSVPVENGERCGVVVEERVVLEEAPARRRRAERESKRLKMEKFDGFE 180
           ID +QMFW EE +  V      R GV+VEE+V+LEEA      A  +          G  
Sbjct: 121 IDYIQMFWREESRGPV------RRGVMVEEKVILEEAGVAATSAAGK---------QGMG 180

Query: 181 RGEFGDCCVCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREI 233
             E   C  C++E     KEV+ I CGH++HKSCILTW Q SNSCPLCR ++
Sbjct: 181 GEECSVCYECYDEEKGGDKEVAKIPCGHMFHKSCILTWFQCSNSCPLCRAKL 216

BLAST of HG10003182 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.0e-07
Identity = 21/50 (42.00%), Postives = 31/50 (62.00%), Query Frame = 0

Query: 183 EFGDCCVCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREI 233
           EF  C VC ++  +   E   + C H+YHK C+L W++  NSCP+CR E+
Sbjct: 220 EFNQCAVCMDDFEE-GTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHEL 268

BLAST of HG10003182 vs. ExPASy Swiss-Prot
Match: Q2HIJ8 (Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RHB1A PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 3.3e-07
Identity = 22/51 (43.14%), Postives = 34/51 (66.67%), Query Frame = 0

Query: 186 DCC-VCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREIKFE 236
           DCC +C E+  DV     T +C H +H SC+L W++RS+ CP+C +E+ F+
Sbjct: 137 DCCPICFEDY-DVENPRLTTKCEHEFHLSCLLEWIERSDRCPICDKEVVFD 186

BLAST of HG10003182 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 3.3e-07
Identity = 24/67 (35.82%), Postives = 39/67 (58.21%), Query Frame = 0

Query: 165 ERESKRLKMEKFDGFERG--EFGDCCVCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRS 224
           E  S RLK  K+    +   +   CCVC EE  +  +++ T++CGH +H  CI  W+++ 
Sbjct: 613 ETISNRLKQRKYKSNTKSPQDAEPCCVCQEEYTE-GEDMGTLECGHEFHSQCIKEWLKQK 672

Query: 225 NSCPLCR 230
           N CP+C+
Sbjct: 673 NLCPICK 678

BLAST of HG10003182 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 5.7e-07
Identity = 21/52 (40.38%), Postives = 32/52 (61.54%), Query Frame = 0

Query: 181 RGEFGDCCVCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREI 233
           + E   C VC +E  D   +V  + C HV+H+ C+L W++  NSCP+CR E+
Sbjct: 210 KSEMNQCAVCMDEFED-GSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

BLAST of HG10003182 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.3e-06
Identity = 19/46 (41.30%), Postives = 31/46 (67.39%), Query Frame = 0

Query: 187 CCVCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREI 233
           C VC +E  +++ E   + C H+YH  CI+ W+ + NSCP+CR+E+
Sbjct: 186 CPVCKDEF-ELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKEL 230

BLAST of HG10003182 vs. ExPASy TrEMBL
Match: A0A6J1G7L1 (NEP1-interacting protein-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111451601 PE=4 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 2.2e-49
Identity = 118/235 (50.21%), Postives = 150/235 (63.83%), Query Frame = 0

Query: 1   MSFATASTFRYSIRSNDGADQSLQIRLGFLWRVLSRSPSAGLPYETLYEIPLSSMRETTF 60
           M FA AST RYS+R N GADQ LQ+RLG+  R++SRSPSAGLPY+ L E P + M +  F
Sbjct: 1   MPFANASTLRYSLRRNHGADQLLQLRLGYSSRLISRSPSAGLPYDILEETPSTPMADALF 60

Query: 61  SLYLRELEDPLLCEAYIAQLLSSLDLGAVTCEIISRKITSVVIEVLESGNYDTKFQIAVE 120
            L LRELEDPL C AY+ +L+SS +LG+VT   I +KITS V +   + +YD KFQ+  +
Sbjct: 61  PLPLRELEDPLFCHAYLVELVSSFNLGSVTWNPIVQKITSFVTQ-WAAVDYDAKFQLVAD 120

Query: 121 IDLVQMFWAEEEQSSVPVENGERCGVVVEERVVLEEAPARRRRAERESKRLKMEKFDGFE 180
           ID +QMFW E+ + SV        GV+VEERVVLEE       A  E + +  E      
Sbjct: 121 IDYIQMFWMEKHRGSV------GRGVMVEERVVLEETGV----AAAEKQGIGGE------ 180

Query: 181 RGEFGDCCVCHEELNDVR---KEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREI 233
                +C VC+E     +   KEV+ I CGH +HKSCILTW QRSNSCPLCR ++
Sbjct: 181 -----ECSVCYESYEAEKGGEKEVAKIPCGHAFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of HG10003182 vs. ExPASy TrEMBL
Match: A0A6J1L191 (E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita maxima OX=3661 GN=LOC111499501 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 1.8e-48
Identity = 111/232 (47.84%), Postives = 152/232 (65.52%), Query Frame = 0

Query: 1   MSFATASTFRYSIRSNDGADQSLQIRLGFLWRVLSRSPSAGLPYETLYEIPLSSMRETTF 60
           M FA +ST RYS+R + G DQ LQ+RLG+  R++S+SPSAGLPY+ L E PL+ + +  F
Sbjct: 1   MLFANSSTLRYSLRRHHGTDQLLQVRLGYSSRLISQSPSAGLPYDILEETPLTPIADALF 60

Query: 61  SLYLRELEDPLLCEAYIAQLLSSLDLGAVTCEIISRKITSVVIEVLESGNYDTKFQIAVE 120
            L LRELEDPL C AY+ +L+SSL+L +++C  I +KITS+V +   + +YD KFQ+ V+
Sbjct: 61  PLPLRELEDPLFCHAYLVELVSSLNLDSMSCNPIIQKITSLVTQ-WAAVDYDAKFQLVVD 120

Query: 121 IDLVQMFWAEEEQSSVPVENGERCGVVVEERVVLEEAPARRRRAERESKRLKMEKFDGFE 180
           ID +QMFW EE +  V      R GV+VEER+VL++A      A   + +       G  
Sbjct: 121 IDYIQMFWREESRGPV------RRGVMVEERIVLDKAGVAATVAAAAAGK------QGMG 180

Query: 181 RGEFGDCCVCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREI 233
             E   C  C++E     KEV+ I CGH++HKSCILTW Q SNSCPLCR ++
Sbjct: 181 GEECSVCYECYDEEKGGDKEVAKIPCGHMFHKSCILTWFQCSNSCPLCRAKL 219

BLAST of HG10003182 vs. ExPASy TrEMBL
Match: A0A6J1G7R8 (E3 ubiquitin-protein ligase MBR2-like OS=Cucurbita moschata OX=3662 GN=LOC111451612 PE=4 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 4.1e-48
Identity = 111/232 (47.84%), Postives = 149/232 (64.22%), Query Frame = 0

Query: 1   MSFATASTFRYSIRSNDGADQSLQIRLGFLWRVLSRSPSAGLPYETLYEIPLSSMRETTF 60
           M FA +ST RYS+R  DG DQ LQ+RLG+  R++SRSP +GLPY+ L E P + + +T F
Sbjct: 1   MLFANSSTLRYSLRRKDGTDQLLQVRLGYSSRLISRSPYSGLPYDILEETPSTLIADTLF 60

Query: 61  SLYLRELEDPLLCEAYIAQLLSSLDLGAVTCEIISRKITSVVIEVLESGNYDTKFQIAVE 120
            L LRELEDPL C AY+ +L+SSL+L +++C  I++KITS+V +   + +YD KFQ+  +
Sbjct: 61  PLPLRELEDPLFCHAYLVELVSSLNLDSISCNPIAQKITSLVTQ-WAAVDYDLKFQLVAD 120

Query: 121 IDLVQMFWAEEEQSSVPVENGERCGVVVEERVVLEEAPARRRRAERESKRLKMEKFDGFE 180
           ID +QMFW EE +  V      R GV+VEE+VVLEEA      A  +          G  
Sbjct: 121 IDYIQMFWREESRGPV------RRGVMVEEKVVLEEAGVAATSAAGK---------QGMG 180

Query: 181 RGEFGDCCVCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREI 233
             E   C  C++E     KEV+ I CGH++HKSCILTW   SNSCPLCR ++
Sbjct: 181 GEECSVCYECYDEEKGGDKEVAKIPCGHMFHKSCILTWFLCSNSCPLCRAKL 216

BLAST of HG10003182 vs. ExPASy TrEMBL
Match: A0A6J1L186 (NEP1-interacting protein-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111499495 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 7.7e-47
Identity = 113/235 (48.09%), Postives = 150/235 (63.83%), Query Frame = 0

Query: 1   MSFATASTFRYSIRSNDGADQSLQIRLGFLWRVLSRSPSAGLPYETLYEIPLSSMRETTF 60
           M  A AST RYS+R N GADQ L+ RL +  R++SRSPSAGLPY+ L E P + M +  F
Sbjct: 1   MPLANASTLRYSLRRNHGADQFLRFRLCYSSRLISRSPSAGLPYDILEETPSTPMADALF 60

Query: 61  SLYLRELEDPLLCEAYIAQLLSSLDLGAVTCEIISRKITSVVIEVLESGNYDTKFQIAVE 120
            L LRELEDPL C AY+ +L+SSL+L +++C  I +KITS+V +   + +YD KFQ+  +
Sbjct: 61  PLPLRELEDPLFCHAYLIELVSSLNLDSMSCNPIVQKITSLVTQ-WAAVDYDAKFQLVAD 120

Query: 121 IDLVQMFWAEEEQSSVPVENGERCGVVVEERVVLEEAPARRRRAERESKRLKMEKFDGFE 180
           ID +QMFW E+   SV        GV+VEE+VVL+EA      A  E + +  E      
Sbjct: 121 IDYIQMFWMEKRPGSV------GRGVMVEEKVVLKEAEV----AAAEKQGIGGE------ 180

Query: 181 RGEFGDCCVCHEELNDVR---KEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREI 233
                +C VC+E     +   +EV+ I CGHV+HKSCILTW QRSNSCPLCR ++
Sbjct: 181 -----ECSVCYESYEAEKGGEREVAKIPCGHVFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of HG10003182 vs. ExPASy TrEMBL
Match: A0A5D3D7E9 (E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001290 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 3.2e-45
Identity = 124/203 (61.08%), Postives = 141/203 (69.46%), Query Frame = 0

Query: 1   MSFATASTFRYSIRSNDGA-DQSLQIRLGFLWRVLSRSPSAGLPYETLYEIPLSSMRETT 60
           M+   ASTFRYSIR  +G+  QSLQIRLG +  +LSR PSAGLPYE L E    S++E T
Sbjct: 1   MTLDGASTFRYSIRRKEGSIQQSLQIRLGRVVSLLSRPPSAGLPYEILKETSSISIQEAT 60

Query: 61  FSLYLRELEDPLLCEAYIAQLLSSLDLGAVTCEIISRKITSVVIEVLESGNYDTKFQIAV 120
           F L LR+LED  LCE YI QLLSS +LG VT  IISRKITS+V+EVL +G+YDTKFQI  
Sbjct: 61  FPLPLRDLEDSCLCEIYIVQLLSSFNLGTVTSGIISRKITSMVVEVLAAGDYDTKFQIVA 120

Query: 121 EIDLVQM-FWAEEEQSS----VPVENGERCGVVVEERVVLEEAPARRR-RAERESKRLKM 180
           EID +QM FW EEE+ S        +G R GVVVEERVVLE  PARRR  AERE K    
Sbjct: 121 EIDHIQMYFWGEEEERSWVGRGNNNSGIRRGVVVEERVVLEGVPARRRAAAEREEK---- 180

Query: 181 EKFDGF-ERG-EFGDCCVCHEEL 195
               GF ERG + GDCCVC EEL
Sbjct: 181 ---GGFDERGDQLGDCCVCREEL 196

BLAST of HG10003182 vs. TAIR 10
Match: AT1G45180.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.6e-09
Identity = 26/67 (38.81%), Postives = 39/67 (58.21%), Query Frame = 0

Query: 165 ERESKRLKMEKFDGFERG--EFGDCCVCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRS 224
           E  S RLK +K+    R   E   CCVC EE  +  +E+  ++CGH +H  CI  W+++ 
Sbjct: 568 ETISNRLKQQKYKSSTRSSQEVEPCCVCQEEYKE-EEEIGRLECGHDFHSQCIKEWLKQK 627

Query: 225 NSCPLCR 230
           N CP+C+
Sbjct: 628 NLCPICK 633

BLAST of HG10003182 vs. TAIR 10
Match: AT4G05350.1 (RING/U-box superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 8.2e-09
Identity = 26/65 (40.00%), Postives = 39/65 (60.00%), Query Frame = 0

Query: 170 RLKMEKFDGFERGEFGDCCVCHEEL--NDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPL 229
           R+K E+F   +  E   C +C E L      ++V+ + C HV+H  C+L W++R N+CPL
Sbjct: 141 RMKAEEFKSLKM-ETEPCSICLESLVSGPKPRDVTRMTCSHVFHNGCLLEWLKRKNTCPL 200

Query: 230 CRREI 233
           CR EI
Sbjct: 201 CRTEI 204

BLAST of HG10003182 vs. TAIR 10
Match: AT5G67120.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.8e-08
Identity = 36/103 (34.95%), Postives = 51/103 (49.51%), Query Frame = 0

Query: 131 EEQSSVPVENG-ERCGVVVEERVVLEEAPARRRRAERESKRLKMEKFDGFERGEFGDCCV 190
           EE  S+  E G ER G+  E   V++E   RR+  +R     K              C +
Sbjct: 186 EELLSITDETGDERTGLSEE---VIDENLIRRKYEKRSDDETKR-------------CVI 245

Query: 191 CHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREI 233
           C ++L D  +EVS + CGH +H  CI  W+  +N CPLC RE+
Sbjct: 246 CQQKLKD-NEEVSKLGCGHDFHFGCIKNWLMVTNKCPLCNREV 271

BLAST of HG10003182 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.0 bits (136), Expect = 2.4e-08
Identity = 21/50 (42.00%), Postives = 33/50 (66.00%), Query Frame = 0

Query: 183 EFGDCCVCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREI 233
           +   C VC EE   V  + + + C H+YHK CI+ W++ +NSCP+CRR++
Sbjct: 220 DMSQCTVCMEEF-IVGGDATELPCKHIYHKDCIVPWLRLNNSCPICRRDL 268

BLAST of HG10003182 vs. TAIR 10
Match: AT4G00335.1 (RING-H2 finger B1A )

HSP 1 Score: 57.0 bits (136), Expect = 2.4e-08
Identity = 22/51 (43.14%), Postives = 34/51 (66.67%), Query Frame = 0

Query: 186 DCC-VCHEELNDVRKEVSTIQCGHVYHKSCILTWVQRSNSCPLCRREIKFE 236
           DCC +C E+  DV     T +C H +H SC+L W++RS+ CP+C +E+ F+
Sbjct: 137 DCCPICFEDY-DVENPRLTTKCEHEFHLSCLLEWIERSDRCPICDKEVVFD 186

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038876865.11.2e-10582.63putative RING-H2 finger protein ATL53 [Benincasa hispida][more]
KAE8650076.13.9e-6963.49hypothetical protein Csa_011336 [Cucumis sativus][more]
XP_038905732.18.9e-6659.23E3 ubiquitin-protein ligase arkadia-A-like [Benincasa hispida][more]
XP_023539480.19.0e-5045.92E3 ubiquitin-protein ligase RNF165-like [Cucurbita pepo subsp. pepo][more]
KAG6605180.12.6e-4948.28E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
P0CH302.0e-0742.00E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q2HIJ83.3e-0743.14Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q9FMM43.3e-0735.82Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q8LPN75.7e-0740.38E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q94AK41.3e-0641.30E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1G7L12.2e-4950.21NEP1-interacting protein-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111451601 PE... [more]
A0A6J1L1911.8e-4847.84E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1G7R84.1e-4847.84E3 ubiquitin-protein ligase MBR2-like OS=Cucurbita moschata OX=3662 GN=LOC111451... [more]
A0A6J1L1867.7e-4748.09NEP1-interacting protein-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111499495 PE=4... [more]
A0A5D3D7E93.2e-4561.08E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1... [more]
Match NameE-valueIdentityDescription
AT1G45180.11.6e-0938.81RING/U-box superfamily protein [more]
AT4G05350.18.2e-0940.00RING/U-box superfamily protein [more]
AT5G67120.11.8e-0834.95RING/U-box superfamily protein [more]
AT1G60360.12.4e-0842.00RING/U-box superfamily protein [more]
AT4G00335.12.4e-0843.14RING-H2 finger B1A [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 154..174
NoneNo IPR availablePANTHERPTHR15710:SF137ZINC FINGER, C3HC4 TYPE (RING FINGER) PROTEINcoord: 146..231
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 146..231
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 186..234
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 187..228
e-value: 1.1E-6
score: 38.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 186..229
e-value: 4.4E-11
score: 42.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 187..229
score: 13.064645
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 139..234
e-value: 3.6E-16
score: 60.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10003182.1HG10003182.1mRNA