HG10002569 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10002569
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionErvatamin-B-like
LocationChr11: 8407509 .. 8408523 (-)
RNA-Seq ExpressionHG10002569
SyntenyHG10002569
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGAAGAGAGACGAGAGACGTACAACCGTTTCAAGGTATTCAAAGACAATGCAAAGTATGTATCAAAAGTGAACCAAATGAACAAAACTTTCAAGTTGAAGCTTAACCAGTTCGCTGATATGTCCAATGATGAGTTCGTGAACTTATATACTAGCTCCAATATTACTTACTACAAAAACCTACATGCCAAGAAAATAGCAGAAGCTATTGATTGGAGGAAAAAAGGAGTTTTTAATGACATCAAAAACCAAGACGCATGTGGTATAATTTATAATTTCGAATTTTGGCTTTATTATTCACCCTCATGATAACTAATCAATGGTTTTGAAAACTAATGGCTTTATTATTCACCCTCATGATAACTAATCAATGGTTTTGAAAACTAATCAATGGTTTTGAAAATATATGTTGAAAACTAATGGTTTTGAAAATATATATTTGCAGGAAGTTGTTGGGCGTTTGTAGCGGTGACTGCCGTTGAAGGAATACACCAAATCAAAATCTCTATCAGAGCAGGAGCTTGGTCAATTGTGATTTTAGGGATGGTCAATTGTGATTTTAGGGATGGAGGTTGCAATGGAGGATTCTATGACTCTGCTTTTGAATTCATTATGCAAAATGGGATCACAACTGAGAAGAACTATCCCTACTATAATAATCATTCATCATTTTTGAAATTTTTTGAAGTTAATTAAGTAAATTATGTGTTTGATTTAATAGAGGAGTAATCTGAAAGTGACAATTGATGGGTATGAGAACGTACCTTCAAACAACGAGGATGCTCTGAAGAAGGCTGTGGCACACCAACCGGTAGCAGTAGCGATAGCGGCCGGTGGACGAGATTTCCAATTATACACCGGGGCAAGTAGTTTTGATCGCTTAATTGTAAATCGTAAGACAAAGATCTTTCGTATCCTGCTAAGAATTAGCCTTCATGCGCCCCAAACCCATCCATGTGTTTTTTTTTCTAAGCATTCTTTAGAAGTAGAAGGTAGTGATTCCTTCTCTTAG

mRNA sequence

ATGGAAGAAGAGAGACGAGAGACGTACAACCGTTTCAAGGTATTCAAAGACAATGCAAAGTATGTATCAAAAGTGAACCAAATGAACAAAACTTTCAAGTTGAAGCTTAACCAGTTCGCTGATATGTCCAATGATGAGTTCGTGAACTTATATACTAGCTCCAATATTACTTACTACAAAAACCTACATGCCAAGAAAATAGCAGAAGCTATTGATTGGAGGAAAAAAGGAGTTTTTAATGACATCAAAAACCAAGACGCATGTGGAAGTTGTTGGGCGTTTGTAGCGGTGACTGCCGTTGAAGGAATACACCAAATCAAAATCTCTATCAGAGCAGGAGCTTGGTCAATTGTGATTTTAGGGATGAGGAGTAATCTGAAAGTGACAATTGATGGGTATGAGAACGTACCTTCAAACAACGAGGATGCTCTGAAGAAGGCTGTGGCACACCAACCGGTAGCAGTAGCGATAGCGGCCGGTGGACGAGATTTCCAATTATACACCGGGGCAAGTAGTTTTGATCGCTTAATTGTAAATCGTAAGACAAAGATCTTTCGTATCCTGCTAAGAATTAGCCTTCATGCGCCCCAAACCCATCCATGTGTTTTTTTTTCTAAGCATTCTTTAGAAGTAGAAGGTAGTGATTCCTTCTCTTAG

Coding sequence (CDS)

ATGGAAGAAGAGAGACGAGAGACGTACAACCGTTTCAAGGTATTCAAAGACAATGCAAAGTATGTATCAAAAGTGAACCAAATGAACAAAACTTTCAAGTTGAAGCTTAACCAGTTCGCTGATATGTCCAATGATGAGTTCGTGAACTTATATACTAGCTCCAATATTACTTACTACAAAAACCTACATGCCAAGAAAATAGCAGAAGCTATTGATTGGAGGAAAAAAGGAGTTTTTAATGACATCAAAAACCAAGACGCATGTGGAAGTTGTTGGGCGTTTGTAGCGGTGACTGCCGTTGAAGGAATACACCAAATCAAAATCTCTATCAGAGCAGGAGCTTGGTCAATTGTGATTTTAGGGATGAGGAGTAATCTGAAAGTGACAATTGATGGGTATGAGAACGTACCTTCAAACAACGAGGATGCTCTGAAGAAGGCTGTGGCACACCAACCGGTAGCAGTAGCGATAGCGGCCGGTGGACGAGATTTCCAATTATACACCGGGGCAAGTAGTTTTGATCGCTTAATTGTAAATCGTAAGACAAAGATCTTTCGTATCCTGCTAAGAATTAGCCTTCATGCGCCCCAAACCCATCCATGTGTTTTTTTTTCTAAGCATTCTTTAGAAGTAGAAGGTAGTGATTCCTTCTCTTAG

Protein sequence

MEEERRETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKKIAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKISIRAGAWSIVILGMRSNLKVTIDGYENVPSNNEDALKKAVAHQPVAVAIAAGGRDFQLYTGASSFDRLIVNRKTKIFRILLRISLHAPQTHPCVFFSKHSLEVEGSDSFS
Homology
BLAST of HG10002569 vs. NCBI nr
Match: XP_038896226.1 (vignain-like [Benincasa hispida])

HSP 1 Score: 200.7 bits (509), Expect = 1.3e-47
Identity = 117/224 (52.23%), Postives = 133/224 (59.38%), Query Frame = 0

Query: 6   RETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAK 65
           RE +NRFKVFK+NAKYV KVNQMNK+ KLKLNQFADMS+DEF+N + SSNITYYKNLHAK
Sbjct: 96  REMHNRFKVFKENAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVSSNITYYKNLHAK 155

Query: 66  KI------------------AEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIK 125
           KI                    +IDWRKKG   DIKNQ  CGSCWAF AV  VEGI+QIK
Sbjct: 156 KIEALGGRVGGFMYEHVEDLPSSIDWRKKGAVTDIKNQGRCGSCWAFAAVAVVEGINQIK 215

Query: 126 ---------------------------------------ISIRAG----AWSIVILGMRS 169
                                                  I+I       A +   L  + 
Sbjct: 216 TNKLVSLSEQEVVDCDYKDGGCGGGFYDSAFEFMMENNGITIEENYPYYAENRYCLAQKG 275

BLAST of HG10002569 vs. NCBI nr
Match: XP_038885798.1 (vignain-like [Benincasa hispida])

HSP 1 Score: 197.2 bits (500), Expect = 1.5e-46
Identity = 115/224 (51.34%), Postives = 132/224 (58.93%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKK 66
           E + RFKVFKDNAKYV KVNQMNK+ KLKLNQFADMS+DEF+N + +SNITYYKNLHAKK
Sbjct: 121 EMHYRFKVFKDNAKYVFKVNQMNKSLKLKLNQFADMSDDEFINTHVNSNITYYKNLHAKK 180

Query: 67  -------------------IAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIK 126
                              +  +IDWRKKG   DIKNQ  CGSCWAF AV  VEGIHQIK
Sbjct: 181 KEAVGGGRVGGFMYEHAEDLPSSIDWRKKGAVTDIKNQGGCGSCWAFAAVAVVEGIHQIK 240

Query: 127 IS--------------IRAG-----------------------------AWSIVILGMRS 169
            +               R G                             A +   L  R+
Sbjct: 241 TNKLVSLSEQEVVDCDYRDGGCGGGFYDSAFEFMMKNNGITTEENYPYYAENRYCLSQRA 300

BLAST of HG10002569 vs. NCBI nr
Match: XP_011658479.1 (ervatamin-B [Cucumis sativus] >KGN48503.1 hypothetical protein Csa_003803 [Cucumis sativus])

HSP 1 Score: 183.7 bits (465), Expect = 1.7e-42
Identity = 113/225 (50.22%), Postives = 127/225 (56.44%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKK 66
           E +NRFKVFK+NAK+V KVN M K+ KLKLNQFADMS+DEF N+Y SSNITYYK+LHAKK
Sbjct: 56  EMHNRFKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMY-SSNITYYKDLHAKK 115

Query: 67  ------------------IAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKI 126
                             I  +IDWRKKG  N IKNQ  CGSCWAF AV AVE IHQIK 
Sbjct: 116 IEATGGRIGGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKT 175

Query: 127 S-------------------IRAGAWSIVILGMRS------------------------- 170
           +                    R G ++     M                           
Sbjct: 176 NELVSLSEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGR 235

BLAST of HG10002569 vs. NCBI nr
Match: KAA0047115.1 (ervatamin-B-like [Cucumis melo var. makuwa] >TYK05040.1 ervatamin-B-like [Cucumis melo var. makuwa])

HSP 1 Score: 182.2 bits (461), Expect = 4.9e-42
Identity = 109/217 (50.23%), Postives = 127/217 (58.53%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNL---- 66
           E + RFKVFKDNAK+V KVNQM ++ KL+LNQFADMS+DEF +++ SSNITYYK+L    
Sbjct: 56  EMHKRFKVFKDNAKHVFKVNQMGRSLKLRLNQFADMSDDEFSSIH-SSNITYYKHLHAKT 115

Query: 67  --------HAKKIAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKIS----- 126
                   HAK+I  +IDWRKKG  N IKNQ  CGSCWAF AV AVE IHQIK +     
Sbjct: 116 VGGSFMYEHAKEIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSL 175

Query: 127 --------------IRAGAWSIVILGMRS-------------------------NLKVTI 168
                          R G ++     M                           N +VTI
Sbjct: 176 SEQEVVDCDYRDGGCRGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTI 235

BLAST of HG10002569 vs. NCBI nr
Match: XP_008454482.1 (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 175.6 bits (444), Expect = 4.6e-40
Identity = 106/218 (48.62%), Postives = 121/218 (55.50%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKK 66
           E + RFKVFKDNAKYV K N M ++ KL+LNQFADMS+DEF +++  SNITYYKNLHAK 
Sbjct: 56  EMHKRFKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAKN 115

Query: 67  -------------IAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKIS--IR 126
                        I  +IDWRKKG  N IKNQ  CGSCWAF AV AVE IHQIK +  + 
Sbjct: 116 GRVGGFMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVS 175

Query: 127 AGAWSIVILGMRS------------------------------------------NLKVT 168
                +V    R                                           N +VT
Sbjct: 176 LSEQEVVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVT 235

BLAST of HG10002569 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.4e-31
Identity = 82/220 (37.27%), Postives = 111/220 (50.45%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKK 66
           E + RF VFK N  +V   N+M+K +KLKLN+FADM+N EF + Y  S + ++K     +
Sbjct: 55  EKHKRFNVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQ 114

Query: 67  -------------IAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKIS--IR 126
                        +  ++DWRKKG   D+K+Q  CGSCWAF  + AVEGI+QIK +  + 
Sbjct: 115 HGSGTFMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVS 174

Query: 127 AGAWSIVILGMRSN-------------------------------------------LKV 169
                +V      N                                           L V
Sbjct: 175 LSEQELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAV 234

BLAST of HG10002569 vs. ExPASy Swiss-Prot
Match: Q9STL4 (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 4.0e-31
Identity = 83/222 (37.39%), Postives = 114/222 (51.35%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNL---- 66
           E   RF VF+ N  +V   N+ N+++KLKLN+FAD++ +EF N YT SNI +++ L    
Sbjct: 53  EREKRFNVFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPK 112

Query: 67  -----------HAKKIAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKIS-- 126
                      +  K+  ++DWRKKG   +IKNQ  CGSCWAF  V AVEGI++IK +  
Sbjct: 113 RGSKQFMYDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKL 172

Query: 127 ------------------IRAGAWSIVILGMRSN-------------------------L 169
                                G   I    ++ N                         +
Sbjct: 173 VSLSEQELVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGV 232

BLAST of HG10002569 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 136.3 bits (342), Expect = 4.0e-31
Identity = 83/220 (37.73%), Postives = 110/220 (50.00%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAK- 66
           E + RF VFK N  +V   N+M+K +KLKLN+FADM+N EF + Y  S + + +      
Sbjct: 55  EKHKRFNVFKANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTP 114

Query: 67  ------------KIAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKIS--IR 126
                        +  ++DWRKKG   D+K+Q  CGSCWAF  V AVEGI+QIK +  + 
Sbjct: 115 HENGAFMYEKVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVA 174

Query: 127 AGAWSIVILGMRSN-------------------------------------------LKV 169
                +V      N                                           L V
Sbjct: 175 LSEQELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAV 234

BLAST of HG10002569 vs. ExPASy Swiss-Prot
Match: O65039 (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.6e-30
Identity = 80/220 (36.36%), Postives = 110/220 (50.00%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLH--- 66
           E   RF VFK NA +V   N+M+K +KLKLN+FADM+N EF N Y+ S + +++      
Sbjct: 53  EKQKRFNVFKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRGGP 112

Query: 67  ----------AKKIAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKISIRAG 126
                        +  ++DWRKKG    +K+Q  CGSCWAF  + AVEGI+QIK +    
Sbjct: 113 RGNGTFMYEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVS 172

Query: 127 A----------------------WSIVILGMRSNL-----------------------KV 169
                                  ++   +  R  +                        V
Sbjct: 173 LSEQELVDCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAV 232

BLAST of HG10002569 vs. ExPASy Swiss-Prot
Match: Q9FGR9 (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 5.8e-30
Identity = 79/220 (35.91%), Postives = 114/220 (51.82%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKK 66
           E   RF VFK N K++ + N+ +K++KLKLN+F DM+++EF   Y  SNI +++    +K
Sbjct: 53  EKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEK 112

Query: 67  -------------IAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIK------ 126
                        +  ++DWRK G    +KNQ  CGSCWAF  V AVEGI+QI+      
Sbjct: 113 KATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTS 172

Query: 127 ----------------------------ISIRAGAWSIVILGMRSNLK-----------V 169
                                       I  + G  S ++   +++ +           V
Sbjct: 173 LSEQELVDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVV 232

BLAST of HG10002569 vs. ExPASy TrEMBL
Match: A0A0A0KGB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 8.1e-43
Identity = 113/225 (50.22%), Postives = 127/225 (56.44%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKK 66
           E +NRFKVFK+NAK+V KVN M K+ KLKLNQFADMS+DEF N+Y SSNITYYK+LHAKK
Sbjct: 56  EMHNRFKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMY-SSNITYYKDLHAKK 115

Query: 67  ------------------IAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKI 126
                             I  +IDWRKKG  N IKNQ  CGSCWAF AV AVE IHQIK 
Sbjct: 116 IEATGGRIGGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKT 175

Query: 127 S-------------------IRAGAWSIVILGMRS------------------------- 170
           +                    R G ++     M                           
Sbjct: 176 NELVSLSEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGR 235

BLAST of HG10002569 vs. ExPASy TrEMBL
Match: A0A5A7U0D8 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00020 PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 2.4e-42
Identity = 109/217 (50.23%), Postives = 127/217 (58.53%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNL---- 66
           E + RFKVFKDNAK+V KVNQM ++ KL+LNQFADMS+DEF +++ SSNITYYK+L    
Sbjct: 56  EMHKRFKVFKDNAKHVFKVNQMGRSLKLRLNQFADMSDDEFSSIH-SSNITYYKHLHAKT 115

Query: 67  --------HAKKIAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKIS----- 126
                   HAK+I  +IDWRKKG  N IKNQ  CGSCWAF AV AVE IHQIK +     
Sbjct: 116 VGGSFMYEHAKEIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSL 175

Query: 127 --------------IRAGAWSIVILGMRS-------------------------NLKVTI 168
                          R G ++     M                           N +VTI
Sbjct: 176 SEQEVVDCDYRDGGCRGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTI 235

BLAST of HG10002569 vs. ExPASy TrEMBL
Match: A0A1S3BRX8 (vignain-like OS=Cucumis melo OX=3656 GN=LOC103492864 PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 2.2e-40
Identity = 108/219 (49.32%), Postives = 125/219 (57.08%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNL---- 66
           E + RFKVFKDNAK V K NQM K+ KLKLNQFADMS+DEF +++ SSNIT+YKNL    
Sbjct: 19  EMHKRFKVFKDNAKQVFKKNQMGKSLKLKLNQFADMSDDEFRSIH-SSNITHYKNLHAKT 78

Query: 67  ----------HAKKIAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKIS--- 126
                     HAK+I  +IDWRKKG  N IK+Q  CGSCWAF AV AVE IHQIK +   
Sbjct: 79  IGRVGGFMYEHAKEIPSSIDWRKKGAVNAIKDQGRCGSCWAFAAVAAVESIHQIKTNELV 138

Query: 127 ----------------IRAGAWSIVILGMRSN-------------------------LKV 168
                            R G ++     +  N                          +V
Sbjct: 139 SLSEQEVVDCDYKDGGCRGGHYNSAFEFIMENDGVTAEDNYPYFEGDGYCRRRGGYKERV 198

BLAST of HG10002569 vs. ExPASy TrEMBL
Match: A0A1S3BYU0 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 2.2e-40
Identity = 106/218 (48.62%), Postives = 121/218 (55.50%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKK 66
           E + RFKVFKDNAKYV K N M ++ KL+LNQFADMS+DEF +++  SNITYYKNLHAK 
Sbjct: 56  EMHKRFKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAKN 115

Query: 67  -------------IAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKIS--IR 126
                        I  +IDWRKKG  N IKNQ  CGSCWAF AV AVE IHQIK +  + 
Sbjct: 116 GRVGGFMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVS 175

Query: 127 AGAWSIVILGMRS------------------------------------------NLKVT 168
                +V    R                                           N +VT
Sbjct: 176 LSEQEVVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVT 235

BLAST of HG10002569 vs. ExPASy TrEMBL
Match: A0A5A7TM64 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003430 PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 1.1e-39
Identity = 105/218 (48.17%), Postives = 122/218 (55.96%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNL---- 66
           E + RFKVFKDNAK+V K N M ++ KL+LNQFADMS+DEF +++  SNITYYKNL    
Sbjct: 57  EMHKRFKVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIH-GSNITYYKNLHAKT 116

Query: 67  ---------HAKKIAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKIS---- 126
                    HAK+I  +IDWRKKG  N IKNQ  CGSCWAF AV AVE IHQIK +    
Sbjct: 117 GHVGGFMYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVS 176

Query: 127 ---------------IRAGAWSIVILGMRS-------------------------NLKVT 168
                           R G ++     M                           N +V 
Sbjct: 177 LSEQEVVDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVK 236

BLAST of HG10002569 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 136.3 bits (342), Expect = 2.9e-32
Identity = 83/222 (37.39%), Postives = 114/222 (51.35%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNL---- 66
           E   RF VF+ N  +V   N+ N+++KLKLN+FAD++ +EF N YT SNI +++ L    
Sbjct: 53  EREKRFNVFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPK 112

Query: 67  -----------HAKKIAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKIS-- 126
                      +  K+  ++DWRKKG   +IKNQ  CGSCWAF  V AVEGI++IK +  
Sbjct: 113 RGSKQFMYDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKL 172

Query: 127 ------------------IRAGAWSIVILGMRSN-------------------------L 169
                                G   I    ++ N                         +
Sbjct: 173 VSLSEQELVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGV 232

BLAST of HG10002569 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 132.5 bits (332), Expect = 4.1e-31
Identity = 79/220 (35.91%), Postives = 114/220 (51.82%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKK 66
           E   RF VFK N K++ + N+ +K++KLKLN+F DM+++EF   Y  SNI +++    +K
Sbjct: 53  EKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEK 112

Query: 67  -------------IAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIK------ 126
                        +  ++DWRK G    +KNQ  CGSCWAF  V AVEGI+QI+      
Sbjct: 113 KATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTS 172

Query: 127 ----------------------------ISIRAGAWSIVILGMRSNLK-----------V 169
                                       I  + G  S ++   +++ +           V
Sbjct: 173 LSEQELVDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVV 232

BLAST of HG10002569 vs. TAIR 10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 129.4 bits (324), Expect = 3.5e-30
Identity = 78/221 (35.29%), Postives = 114/221 (51.58%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNITYYKNLHAKK 66
           E   RF VF+ N  +V + N+ NK +KLK+N+FAD+++ EF + Y  SN+ +++ L   K
Sbjct: 53  EAIKRFNVFRHNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPK 112

Query: 67  -------------IAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQIKIS---- 126
                        +  ++DWR+KG   ++KNQ  CGSCWAF  V AVEGI++I+ +    
Sbjct: 113 RGSGGFMYENVTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVS 172

Query: 127 --------------------IRAGAWSIV------------------ILGMRSNL----K 169
                               +   A+  +                  +   R+N      
Sbjct: 173 LSEQELVDCDTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGET 232

BLAST of HG10002569 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.3e-29
Identity = 83/228 (36.40%), Postives = 118/228 (51.75%), Query Frame = 0

Query: 4   ERRETYN-------RFKVFKDNAKYVSKVNQM-NKTFKLKLNQFADMSNDEFVNLYTSSN 63
           E R+ YN       RF++FKDN K+V + + + N+T+++ L +FAD++NDEF  +Y  S 
Sbjct: 49  ENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRSK 108

Query: 64  I---------TYYKNLHAKKIAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQI 123
           +           Y       + +AIDWR KG  N +K+Q +CGSCWAF A+ AVEGI+QI
Sbjct: 109 MERTRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQI 168

Query: 124 KI------------------------SIRAGAWSIVI----------------------L 169
           K                          +   A+  +I                       
Sbjct: 169 KTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATDVNVCNS 228

BLAST of HG10002569 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 127.1 bits (318), Expect = 1.7e-29
Identity = 79/215 (36.74%), Postives = 115/215 (53.49%), Query Frame = 0

Query: 7   ETYNRFKVFKDNAKYVSKVNQMNKTFKLKLNQFADMSNDEFVNLYTSSNI---------T 66
           E   RF++FKDN ++V + N+ N +++L L +FAD++NDE+ + Y  + +          
Sbjct: 68  EKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKKGERRTSL 127

Query: 67  YYKNLHAKKIAEAIDWRKKGVFNDIKNQDACGSCWAFVAVTAVEGIHQI----------- 126
            Y+     ++ E+IDWRKKG   ++K+Q  CGSCWAF  + AVEGI+QI           
Sbjct: 128 RYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLITLSEQ 187

Query: 127 -----KISIRAG--------AWSIVILG--------------------MRSNLK-VTIDG 168
                  S   G        A+  +I                      +R N K VTID 
Sbjct: 188 ELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKVVTIDS 247

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038896226.11.3e-4752.23vignain-like [Benincasa hispida][more]
XP_038885798.11.5e-4651.34vignain-like [Benincasa hispida][more]
XP_011658479.11.7e-4250.22ervatamin-B [Cucumis sativus] >KGN48503.1 hypothetical protein Csa_003803 [Cucum... [more]
KAA0047115.14.9e-4250.23ervatamin-B-like [Cucumis melo var. makuwa] >TYK05040.1 ervatamin-B-like [Cucumi... [more]
XP_008454482.14.6e-4048.62PREDICTED: ervatamin-B-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
P124122.4e-3137.27Vignain OS=Vigna mungo OX=3915 PE=1 SV=1[more]
Q9STL44.0e-3137.39KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ... [more]
P258034.0e-3137.73Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2[more]
O650392.6e-3036.36Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1[more]
Q9FGR95.8e-3035.91KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KGB18.1e-4350.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1[more]
A0A5A7U0D82.4e-4250.23Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00... [more]
A0A1S3BRX82.2e-4049.32vignain-like OS=Cucumis melo OX=3656 GN=LOC103492864 PE=3 SV=1[more]
A0A1S3BYU02.2e-4048.62ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1[more]
A0A5A7TM641.1e-3948.17Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G0034... [more]
Match NameE-valueIdentityDescription
AT3G48340.12.9e-3237.39Cysteine proteinases superfamily protein [more]
AT5G50260.14.1e-3135.91Cysteine proteinases superfamily protein [more]
AT3G48350.13.5e-3035.29Cysteine proteinases superfamily protein [more]
AT3G19390.11.3e-2936.40Granulin repeat cysteine protease family protein [more]
AT1G47128.11.7e-2936.74Granulin repeat cysteine protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 1..47
e-value: 7.4E-4
score: 28.8
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 6..47
e-value: 8.8E-10
score: 38.9
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 67..197
e-value: 1.8E-7
score: 9.4
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 127..170
e-value: 4.3E-6
score: 27.0
coord: 68..108
e-value: 2.1E-11
score: 44.3
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 1..115
e-value: 4.6E-30
score: 107.3
coord: 116..185
e-value: 9.5E-10
score: 40.5
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 127..168
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 6..107
NoneNo IPR availablePANTHERPTHR12411:SF745CYSTEINE PROTEASE RDL2-RELATEDcoord: 6..107
coord: 127..168
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 6..169

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10002569.1HG10002569.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0008234 cysteine-type peptidase activity