HG10002553 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10002553
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Pectinesterase
Location	Chr11: 8183969 .. 8187345 (-)
RNA-Seq Expression	HG10002553
Synteny	HG10002553

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTCCAAAATCTAAACCGAACGTGACATTTCAAGGACAAAGCTATACAACAACCGCCATTGTTTGGAATGACACTGCCAATTCTTCCCATGGCACCTTTTATAGTGGCTCTGTCCAAGTCTTCTCTTCCAATTTCATTGCCAAAAATATAAGCTTCATGGTAAATTCTAATTATCAATATTTATCTTTCTTTATCCACCTTTTATTATTATGGTTAAAAAATGTTTTTAGTCCCTAAACTTTCATGAAAGTAGAAATTTAGTCTCTGAACTTTGATTTGTAACAATTTAGTCTTTGTACTTTCCATGTTGTAACAATTTAATCCTTAAACTTTAGTAGGTAGCAATTTAGTCTTTGTACTTTCAAATTTGTGACAATTTAGTCCATAACGCAAAAAAAATTCATCAAAATTATATGTCAATTTTTATTATTTTATGATGTAGACTTTATATTTTATAAAAATATTGAGTCTCTAATTAAGTTATCGATTTATTTATGCAAGAAATTTTATTAAATCTTAACCCTAATTTCGACAATAGAGACTAAATCGTTAAAAATTTTAAAGTATAGGAATTAAATTGTTACATAATATAGTTCAGGGACTAAATTGTTACAAACATGAAAATACAGGGACTAAATCGTTACAAACTAAAGCTCATGAACTAAATTGTTACTTTCATAAAAGTTTAGGGACCAAAAGTAATTTTTAACCTATTATTATTGATTATATTACAAATTTTAGGTAATCATTTGAGTTCTATTTGACAATATCTTGTCTTTCGTTTTTTAGTTTTTGAAAATTAAGCCTGTAAACATTTATTTTACCTCTAAATTTATTGTTCTGTTACCTACATTTTACCAATATTTTTCATTTGGTAACTCTTTAGGCTGAGTAACAATTTGATTTTTGGTTTTTAAAATTAAGCCTATATACAATCATTTCACTTCTAAATTTATTGTGTTATTATCTTTTTTTTTTTTTTATCTATGTTTTCAAAAACTAAGTCAAAATTTGAAAACTGAAAAAAGTAGTTATTAAATATTTGTTTTTGTTTTTGAAATTTAGCTAAGAATTCAACTCTTATACTTAAGAAATGTGCATGTCATAGTAAAAAATTGAAAAGAAATAAACTTAATTTTCAAAAACAAAAAATTAAATTGTTACGGCATTAAATTTTTGTTTTTGGTTTCTAGGTTTTTTTTTTTCATTTTTATTGAGTTTTTCTTGACATCTGTTTTTTCCTTGGAATTTTAGATAAAATTTAATTTTGAGATTATTTATTAACCATTTTGATCTTTGTTTCTTATGATTTATTTAAAGATTAAAATACTAGTTTAGTTTCTATATTTTGAGATTTGTTCGATTTTAGTTATGTACTTTTAAATGTTCAATTTTAATCTATATACTTTTAATAAATCTTAAATTTAGTCCCTCAAAATTAATCTTTATCAAAATTTGTTAAATAATAATAATAATAATAATTTTTATTCAAAGAAATATAAAATGTTAAAAAATAACAAAAAAAATTTTAAAAATCATTAAAAAAATTAGGTTTAAATCTCATTTTCGTCCTTAATTTTTTAAAATTTTTAAACTTATAAAACGTTTATTTTAGTCCCAAAACTTATGTCTATTTTAGTTTCTGAGCCTTTTAAAAACGACTACTTTTGGTATATTATATTATTTTTTTTATCTACTCCTCATTTCTCTAATACTTAAATCTAATCTATGTGGCTTGAATTCACCCTCCTACTAACATCATAGTACACATATTTAAAGTTATATTAATTTGTTAAAAAATAAAATTTTAAAAAAATCAAAATAGTCGATTTAAATGTTCAATTATCAAAATTAAACCAAATCTAAAAGTTTGAAAGTTGGGAAAGTTAATAAATTTGAAATTTTAGTTACTAAATTAAAATTTTATTTAAAAAATCCTATTTAAATTTTCCACATTATTAAATGAACTAATAATTTTAAATCCAGAAAGAAATTCACCTACCCATAAAATTTTATTTTTTTTAAAAAAAAACCCAAAATTCAATAATATAGATTATCCTCACTTAATTTTGAGCAAATATTTTTGGGGCTTTGGCAGAATGTGGCGCCAATTCCTGGGCCGGGCGACGTCGGAGCGCAGGCGGTGGCGATTAGAATCGGCGGAGACCAGGCGGCGTTTTGGAGCTGTGGATTCTTCGGCGCTCAAGACACTCTCCACGACGATCGTGGCCGCCATTACTTCAAAGATTGCTACATTCAAGGCTCCATCGATTTCATCTTCGGAAACGGAAGATCCTTCTACGAGGTAAATCGGAGCCCTAACTTTTTCCATGACTTCGATTTGACGATGAATTTCAGAATCGCGATTTTGAAAATCTTGTGACGCCGTATTGTAGAGTTGCCAGTTGATTTCGATGGCGAATCCGGTTCCGCAAGGATCGAAGTTTGTGAACGGTGCGGTGACGGCGCACGGCAGAGCCTCCGCTGACGAGAACTCTGGATTTGCTTTTGTTAATTGCAGCATCGGCGGGACCGGTAGAGTGTGGCTCGGCCGCGCGTGGCGGCCGTTCTCTCGCGTCGTCTTTGCTAACACCGTCATGACGGATATTATTGCGCCGGAGGGGTGGAATGACTTTAATGACCCTGCAAGGGACCAGTACGTTTTCTTAATTTTAAACTTAAAAAAAGAAAGAAGGTTAAATTATTAAGTTTGTTTCATTTTTTGTTTTTCAAGGAAAGTTTGTTTCAATTATACACTTCAATTTCAATATTTAGCTCTCACTCCATAAACATTTGATTTAAAAAAAAAAAATTGAATTTAAAATAATTGTAATGAATAGCAATTTTAGAGATAATAACCGGGTGTATAGCAACATCTAAAAAAACAAAATATAGCAATTTTTTAAAATTTTGATATATATGCTAATACTTTGAATCTGATGGCTATATTTATAAGTTTCCCTTAAATTTATAAACACTATTATACTTCTTTAGAATTTAAGTGTGTATAGACTTCTACAAAATAAAATTGACGATTATAATTTGAATGGACAAGTTTCAAATAAATCTTAATTGAGAGTGTAAATTGATTCGCTTACGTAAGTTTAGAGATTTAATTGATAAAATTACAAGTCTAACCTAGGAGGTTTATCTAAATAAAACATAAGTATTACTATAGTTGAAAGGATTGATTTGGTGTGTTGTGAAAATGCAGGACAATCTTCTATGGAGAGTACAACTGCAGTGGAACAGGAGCCAATACGAGCTCAAGGGCTCCCTTTGTTCAGAGGCTTAATGATACTCAAGTTTCCCCTTTCCTCAACTTGTCTTTCATTGATGCTGACCAATGGCTCCAACCTTATTCATTGTTCTAA

mRNA sequence

ATGGTTCCAAAATCTAAACCGAACGTGACATTTCAAGGACAAAGCTATACAACAACCGCCATTGTTTGGAATGACACTGCCAATTCTTCCCATGGCACCTTTTATAGTGGCTCTGTCCAAGTCTTCTCTTCCAATTTCATTGCCAAAAATATAAGCTTCATGAATGTGGCGCCAATTCCTGGGCCGGGCGACGTCGGAGCGCAGGCGGTGGCGATTAGAATCGGCGGAGACCAGGCGGCGTTTTGGAGCTGTGGATTCTTCGGCGCTCAAGACACTCTCCACGACGATCGTGGCCGCCATTACTTCAAAGATTGCTACATTCAAGGCTCCATCGATTTCATCTTCGGAAACGGAAGATCCTTCTACGAGAGTTGCCAGTTGATTTCGATGGCGAATCCGGTTCCGCAAGGATCGAAGTTTGTGAACGGTGCGGTGACGGCGCACGGCAGAGCCTCCGCTGACGAGAACTCTGGATTTGCTTTTGTTAATTGCAGCATCGGCGGGACCGGTAGAGTGTGGCTCGGCCGCGCGTGGCGGCCGTTCTCTCGCGTCGTCTTTGCTAACACCGTCATGACGGATATTATTGCGCCGGAGGGGTGGAATGACTTTAATGACCCTGCAAGGGACCAGACAATCTTCTATGGAGAGTACAACTGCAGTGGAACAGGAGCCAATACGAGCTCAAGGGCTCCCTTTGTTCAGAGGCTTAATGATACTCAAGTTTCCCCTTTCCTCAACTTGTCTTTCATTGATGCTGACCAATGGCTCCAACCTTATTCATTGTTCTAA

Coding sequence (CDS)

ATGGTTCCAAAATCTAAACCGAACGTGACATTTCAAGGACAAAGCTATACAACAACCGCCATTGTTTGGAATGACACTGCCAATTCTTCCCATGGCACCTTTTATAGTGGCTCTGTCCAAGTCTTCTCTTCCAATTTCATTGCCAAAAATATAAGCTTCATGAATGTGGCGCCAATTCCTGGGCCGGGCGACGTCGGAGCGCAGGCGGTGGCGATTAGAATCGGCGGAGACCAGGCGGCGTTTTGGAGCTGTGGATTCTTCGGCGCTCAAGACACTCTCCACGACGATCGTGGCCGCCATTACTTCAAAGATTGCTACATTCAAGGCTCCATCGATTTCATCTTCGGAAACGGAAGATCCTTCTACGAGAGTTGCCAGTTGATTTCGATGGCGAATCCGGTTCCGCAAGGATCGAAGTTTGTGAACGGTGCGGTGACGGCGCACGGCAGAGCCTCCGCTGACGAGAACTCTGGATTTGCTTTTGTTAATTGCAGCATCGGCGGGACCGGTAGAGTGTGGCTCGGCCGCGCGTGGCGGCCGTTCTCTCGCGTCGTCTTTGCTAACACCGTCATGACGGATATTATTGCGCCGGAGGGGTGGAATGACTTTAATGACCCTGCAAGGGACCAGACAATCTTCTATGGAGAGTACAACTGCAGTGGAACAGGAGCCAATACGAGCTCAAGGGCTCCCTTTGTTCAGAGGCTTAATGATACTCAAGTTTCCCCTTTCCTCAACTTGTCTTTCATTGATGCTGACCAATGGCTCCAACCTTATTCATTGTTCTAA

Protein sequence

MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQVSPFLNLSFIDADQWLQPYSLF

Homology

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BLAST of HG10002553 vs. NCBI nr
Match: XP_008451885.1 (PREDICTED: probable pectinesterase 8 [Cucumis melo])

HSP 1 Score: 531.2 bits (1367), Expect = 5.1e-147
Identity = 251/262 (95.80%), Postives = 256/262 (97.71%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 60
           MVPKSKPNVTFQGQ YTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP
Sbjct: 133 MVPKSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 192

Query: 61  GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 120
           GPGDVGAQAVAIR+GGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS
Sbjct: 193 GPGDVGAQAVAIRVGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 252

Query: 121 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 180
           FYESCQL+SMANPVPQGSKFVNGAVTAHGRASA+ENSGF+FVNCSIGGTGRVWLGRAWRP
Sbjct: 253 FYESCQLVSMANPVPQGSKFVNGAVTAHGRASAEENSGFSFVNCSIGGTGRVWLGRAWRP 312

Query: 181 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQ 240
           FSRVVFANTVMTDIIAPEGWNDFNDP RDQTIFYGEYNCSG GAN SSRAP+VQRLNDTQ
Sbjct: 313 FSRVVFANTVMTDIIAPEGWNDFNDPTRDQTIFYGEYNCSGAGANMSSRAPYVQRLNDTQ 372

Query: 241 VSPFLNLSFIDADQWLQPYSLF 263
           VS FLNLSFIDADQWLQPYS F
Sbjct: 373 VSSFLNLSFIDADQWLQPYSFF 394

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BLAST of HG10002553 vs. NCBI nr
Match: XP_038903629.1 (probable pectinesterase 8 [Benincasa hispida])

HSP 1 Score: 530.4 bits (1365), Expect = 8.7e-147
Identity = 252/262 (96.18%), Postives = 255/262 (97.33%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 60
           +VPKSK NVTFQGQ YTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP
Sbjct: 140 IVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 199

Query: 61  GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 120
           GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS
Sbjct: 200 GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 259

Query: 121 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 180
           FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGF FVNCSIGGTGRVWLGRAWRP
Sbjct: 260 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFVFVNCSIGGTGRVWLGRAWRP 319

Query: 181 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQ 240
           FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNC+G GAN SSRAPFVQRLNDTQ
Sbjct: 320 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCNGAGANMSSRAPFVQRLNDTQ 379

Query: 241 VSPFLNLSFIDADQWLQPYSLF 263
            SPFLNLSFIDADQWLQPY+ F
Sbjct: 380 ASPFLNLSFIDADQWLQPYAFF 401

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BLAST of HG10002553 vs. NCBI nr
Match: XP_004147773.1 (probable pectinesterase 8 [Cucumis sativus] >KGN53483.1 hypothetical protein Csa_014669 [Cucumis sativus])

HSP 1 Score: 525.0 bits (1351), Expect = 3.7e-145
Identity = 249/262 (95.04%), Postives = 254/262 (96.95%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 60
           MVPK+K NVTFQGQ YTTTAIVWNDTANSSHGTFYS SVQVFSSNFIAKN+SFMNVAPIP
Sbjct: 133 MVPKTKANVTFQGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNFIAKNLSFMNVAPIP 192

Query: 61  GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 120
            PGDVGAQ VAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS
Sbjct: 193 APGDVGAQGVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 252

Query: 121 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 180
           FYESCQL+SMANPVPQGSKFVNGAVTAHGRASADENSGF+FVNCSIGGTGRVWLGRAWRP
Sbjct: 253 FYESCQLVSMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGRVWLGRAWRP 312

Query: 181 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQ 240
           FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSG GAN SSRAP+VQRLNDTQ
Sbjct: 313 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANMSSRAPYVQRLNDTQ 372

Query: 241 VSPFLNLSFIDADQWLQPYSLF 263
           VS FLNLSFIDADQWLQPYSLF
Sbjct: 373 VSSFLNLSFIDADQWLQPYSLF 394

[Close]

BLAST of HG10002553 vs. NCBI nr
Match: XP_022931352.1 (probable pectinesterase 8 [Cucurbita moschata])

HSP 1 Score: 523.1 bits (1346), Expect = 1.4e-144
Identity = 247/259 (95.37%), Postives = 254/259 (98.07%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 60
           MVP+SKPNVTFQGQ YTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP
Sbjct: 123 MVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 182

Query: 61  GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 120
           GPG +GAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS
Sbjct: 183 GPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 242

Query: 121 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 180
           +YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP
Sbjct: 243 YYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 302

Query: 181 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQ 240
           FSRVVFANTVMTDIIA EGWNDFNDPARDQTIFYGEYNCSG GAN S+RAP+VQRLNDTQ
Sbjct: 303 FSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQ 362

Query: 241 VSPFLNLSFIDADQWLQPY 260
           VSPFLNLSFIDADQWLQPY
Sbjct: 363 VSPFLNLSFIDADQWLQPY 381

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BLAST of HG10002553 vs. NCBI nr
Match: KAG7015100.1 (putative pectinesterase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 521.5 bits (1342), Expect = 4.1e-144
Identity = 246/259 (94.98%), Postives = 253/259 (97.68%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 60
           MVP+SKPNVTFQGQ YTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP
Sbjct: 123 MVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 182

Query: 61  GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 120
           GPG +GAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS
Sbjct: 183 GPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 242

Query: 121 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 180
           +YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP
Sbjct: 243 YYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 302

Query: 181 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQ 240
           FSRVVFANTVMTDIIA EGWNDFNDPARDQTIFYGEYNCSG GAN S+RAP+VQRLNDTQ
Sbjct: 303 FSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQ 362

Query: 241 VSPFLNLSFIDADQWLQPY 260
            SPFLNLSFIDADQWLQPY
Sbjct: 363 ASPFLNLSFIDADQWLQPY 381

[Close]

BLAST of HG10002553 vs. ExPASy Swiss-Prot
Match: O23038 (Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2)

HSP 1 Score: 421.8 bits (1083), Expect = 5.7e-117
Identity = 193/257 (75.10%), Postives = 224/257 (87.16%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 60
           ++PK+KPN+T QGQ +  TAI WNDTA S++GTFY  +VQVF S F+AKNISFMNVAPIP
Sbjct: 133 VIPKTKPNITLQGQGFDITAIAWNDTAYSANGTFYCATVQVFGSQFVAKNISFMNVAPIP 192

Query: 61  GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 120
            PGDVGAQAVAIRI GD++AF  CGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGN +S
Sbjct: 193 KPGDVGAQAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKS 252

Query: 121 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 180
            Y+ C++ISMAN +  GSK VNGAVTA+GR+S DENSGF+FVNC+IGGTG VWLGRAWRP
Sbjct: 253 LYQDCRIISMANQLSPGSKAVNGAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRP 312

Query: 181 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQ 240
           +SRVVF +T MTD+IAPEGWN+FNDP+RD TIFYGEYNCSG GA+ S RAP+VQ+LN+TQ
Sbjct: 313 YSRVVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQ 372

Query: 241 VSPFLNLSFIDADQWLQ 258
           V+  +N SFID DQWLQ
Sbjct: 373 VALLINTSFIDGDQWLQ 389

[Close]

BLAST of HG10002553 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 4.0e-78
Identity = 139/256 (54.30%), Postives = 185/256 (72.27%), Query Frame = 0


Query: 2   VPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIPG 61
           V ++K N+  QG+ Y  T+I WNDTA S+  T  S S  VF++NF A NISF N AP P 
Sbjct: 134 VNENKTNLVIQGRGYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPD 193

Query: 62  PGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSF 121
           PG+  AQAVA+RI GDQAAF+ CGF+GAQDTL DD+GRH+FK+C+IQGSIDFIFGNGRS 
Sbjct: 194 PGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSL 253

Query: 122 YESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPF 181
           Y+ C + S+A     G   V G++TA GR S DE SGF+FVNC I G+G + LGRAW  +
Sbjct: 254 YQDCTINSIAKGNTSG---VTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAY 313

Query: 182 SRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQV 241
           + VVF+NT M+ II PEGWN++ D  +++T+ +GE+ C G GA+   R  F ++L D++ 
Sbjct: 314 ATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEA 373

Query: 242 SPFLNLSFIDADQWLQ 258
           S F+++SFID D+WL+
Sbjct: 374 SSFIDVSFIDGDEWLR 386

[Close]

BLAST of HG10002553 vs. ExPASy Swiss-Prot
Match: Q9ZQA4 (Putative pectinesterase 14 OS=Arabidopsis thaliana OX=3702 GN=PME14 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 2.6e-77
Identity = 133/256 (51.95%), Postives = 180/256 (70.31%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 60
           +V ++K N+  QG  Y+ T+I WN+T  SS+GTF S SV VF   F A NISF N AP P
Sbjct: 84  IVHENKNNLVVQGMGYSRTSIEWNNTTASSNGTFSSFSVAVFGEKFTAYNISFKNTAPAP 143

Query: 61  GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 120
            PG V AQAVA+++ GD+AAF+ CGF+G QDTL D  GRH+FK C+I+GSIDFIFGNGRS
Sbjct: 144 NPGAVDAQAVALKVVGDKAAFYGCGFYGNQDTLLDQEGRHFFKGCFIEGSIDFIFGNGRS 203

Query: 121 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 180
            YE C L S+A       +   G +TA+G+ +  + +GF FVNC I G+ RVWLGRAWRP
Sbjct: 204 LYEDCTLHSIA------KENTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRP 263

Query: 181 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQ 240
           ++RV+F+ T M+ +++ +GWND  DP   +T++YGE+ C G GAN S R  + + L+D +
Sbjct: 264 YARVIFSKTYMSRVVSLDGWNDMGDPKTQRTVYYGEHRCYGPGANHSKRVTYAKLLSDVE 323

Query: 241 VSPFLNLSFIDADQWL 257
            +PF N+SFID ++WL
Sbjct: 324 AAPFTNISFIDGEEWL 333

[Close]

BLAST of HG10002553 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 2.8e-71
Identity = 128/262 (48.85%), Postives = 173/262 (66.03%), Query Frame = 0


Query: 2   VPKSKPNVTFQGQSYTTTAIVWNDTANSSH------GTFYSGSVQVFSSNFIAKNISFMN 61
           +P  K  +T +G+    T + W DTA +        GT+ S S  V S  F+AKNI+F N
Sbjct: 129 IPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRN 188

Query: 62  VAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIF 121
             P+P PG VG QAVA+R+  D AAF+ C   GAQDTL+D  GRHY+KDCYI+GS+DFIF
Sbjct: 189 TTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIF 248

Query: 122 GNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLG 181
           GN  S YE C + ++A+ +        GAVTA GR+S  E++GF+FV C + GTG ++LG
Sbjct: 249 GNALSLYEGCHVHAIADKL--------GAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLG 308

Query: 182 RAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQR 241
           RAW PFSRVVFA T M +II P GW ++ DP+R+ T+FYG+Y C+G GAN   R  + + 
Sbjct: 309 RAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARE 368

Query: 242 LNDTQVSPFLNLSFIDADQWLQ 258
           L D +  PFL+L+FID  +W++
Sbjct: 369 LTDEEAKPFLSLTFIDGSEWIK 382

[Close]

BLAST of HG10002553 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 3.1e-62
Identity = 123/265 (46.42%), Postives = 173/265 (65.28%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTAN--SSHG----TFYSGSVQVFSSNFIAKNISFM 60
           +VP +KP +TF+G     TAI W+D A+   ++G    T+ + SV V+++ F A+NISF 
Sbjct: 103 VVPATKPYITFKGAGRDVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFT 162

Query: 61  NVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFI 120
           N AP P PG  G QAVA RI GD+A F  CGF+GAQDTL DD GRHYFK+CYI+GSIDFI
Sbjct: 163 NTAPAPLPGMQGWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFI 222

Query: 121 FGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWL 180
           FGNGRS Y+ C+L S+A      S+F  G++ AHGR   +E +GFAFV C + GTG +++
Sbjct: 223 FGNGRSMYKDCELHSIA------SRF--GSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYV 282

Query: 181 GRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPA-RDQTIFYGEYNCSGTGANTSSRAPFV 240
           GRA   +SR+V+A T    ++A  GW+D++  + + +T F+G YNC G GA  +    + 
Sbjct: 283 GRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWA 342

Query: 241 QRLNDTQVSPFLNLSFIDADQWLQP 259
           + L+     PF+  SF++   W+ P
Sbjct: 343 RALDYESAHPFIAKSFVNGRHWIAP 359

[Close]

BLAST of HG10002553 vs. ExPASy TrEMBL
Match: A0A1S3BRY7 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493043 PE=4 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 2.5e-147
Identity = 251/262 (95.80%), Postives = 256/262 (97.71%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 60
           MVPKSKPNVTFQGQ YTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP
Sbjct: 133 MVPKSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 192

Query: 61  GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 120
           GPGDVGAQAVAIR+GGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS
Sbjct: 193 GPGDVGAQAVAIRVGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 252

Query: 121 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 180
           FYESCQL+SMANPVPQGSKFVNGAVTAHGRASA+ENSGF+FVNCSIGGTGRVWLGRAWRP
Sbjct: 253 FYESCQLVSMANPVPQGSKFVNGAVTAHGRASAEENSGFSFVNCSIGGTGRVWLGRAWRP 312

Query: 181 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQ 240
           FSRVVFANTVMTDIIAPEGWNDFNDP RDQTIFYGEYNCSG GAN SSRAP+VQRLNDTQ
Sbjct: 313 FSRVVFANTVMTDIIAPEGWNDFNDPTRDQTIFYGEYNCSGAGANMSSRAPYVQRLNDTQ 372

Query: 241 VSPFLNLSFIDADQWLQPYSLF 263
           VS FLNLSFIDADQWLQPYS F
Sbjct: 373 VSSFLNLSFIDADQWLQPYSFF 394

[Close]

BLAST of HG10002553 vs. ExPASy TrEMBL
Match: A0A0A0KVA9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G056710 PE=4 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 1.8e-145
Identity = 249/262 (95.04%), Postives = 254/262 (96.95%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 60
           MVPK+K NVTFQGQ YTTTAIVWNDTANSSHGTFYS SVQVFSSNFIAKN+SFMNVAPIP
Sbjct: 133 MVPKTKANVTFQGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNFIAKNLSFMNVAPIP 192

Query: 61  GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 120
            PGDVGAQ VAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS
Sbjct: 193 APGDVGAQGVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 252

Query: 121 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 180
           FYESCQL+SMANPVPQGSKFVNGAVTAHGRASADENSGF+FVNCSIGGTGRVWLGRAWRP
Sbjct: 253 FYESCQLVSMANPVPQGSKFVNGAVTAHGRASADENSGFSFVNCSIGGTGRVWLGRAWRP 312

Query: 181 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQ 240
           FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSG GAN SSRAP+VQRLNDTQ
Sbjct: 313 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANMSSRAPYVQRLNDTQ 372

Query: 241 VSPFLNLSFIDADQWLQPYSLF 263
           VS FLNLSFIDADQWLQPYSLF
Sbjct: 373 VSSFLNLSFIDADQWLQPYSLF 394

[Close]

BLAST of HG10002553 vs. ExPASy TrEMBL
Match: A0A6J1ETE2 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437565 PE=4 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 6.8e-145
Identity = 247/259 (95.37%), Postives = 254/259 (98.07%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 60
           MVP+SKPNVTFQGQ YTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP
Sbjct: 123 MVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 182

Query: 61  GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 120
           GPG +GAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS
Sbjct: 183 GPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 242

Query: 121 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 180
           +YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP
Sbjct: 243 YYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 302

Query: 181 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQ 240
           FSRVVFANTVMTDIIA EGWNDFNDPARDQTIFYGEYNCSG GAN S+RAP+VQRLNDTQ
Sbjct: 303 FSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGANMSARAPYVQRLNDTQ 362

Query: 241 VSPFLNLSFIDADQWLQPY 260
           VSPFLNLSFIDADQWLQPY
Sbjct: 363 VSPFLNLSFIDADQWLQPY 381

[Close]

BLAST of HG10002553 vs. ExPASy TrEMBL
Match: A0A6J1J3X1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483170 PE=4 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 2.6e-144
Identity = 246/259 (94.98%), Postives = 253/259 (97.68%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 60
           MVP+SKPNVTFQGQ Y TTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP
Sbjct: 124 MVPRSKPNVTFQGQGYKTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 183

Query: 61  GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 120
           GPG +GAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS
Sbjct: 184 GPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 243

Query: 121 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 180
           +YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP
Sbjct: 244 YYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 303

Query: 181 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQ 240
           FSRVVFANTVMTDIIA EGWNDFNDPARDQTIFYGEYNCSGTGAN S+RAP+VQRLNDTQ
Sbjct: 304 FSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGTGANMSARAPYVQRLNDTQ 363

Query: 241 VSPFLNLSFIDADQWLQPY 260
            SPFLNLSFIDADQWLQPY
Sbjct: 364 ASPFLNLSFIDADQWLQPY 382

[Close]

BLAST of HG10002553 vs. ExPASy TrEMBL
Match: A0A6J1C3U9 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008131 PE=4 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 1.3e-143
Identity = 245/259 (94.59%), Postives = 250/259 (96.53%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 60
           MVP+SK NVTF+GQ YTTTAIVWNDTANSSHGTFYS SVQVFSSNFIAKNISFMNVAPIP
Sbjct: 135 MVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASVQVFSSNFIAKNISFMNVAPIP 194

Query: 61  GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 120
           GPGDVGAQAVA+RIGGD AAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS
Sbjct: 195 GPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 254

Query: 121 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 180
           FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCS+ GTGRVWLGRAWRP
Sbjct: 255 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSVAGTGRVWLGRAWRP 314

Query: 181 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQ 240
           FSRVVF NTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSG GANT +RAPFVQRLNDTQ
Sbjct: 315 FSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGAGANTGARAPFVQRLNDTQ 374

Query: 241 VSPFLNLSFIDADQWLQPY 260
           VSPFLNLSFIDADQWL PY
Sbjct: 375 VSPFLNLSFIDADQWLHPY 393

[Close]

BLAST of HG10002553 vs. TAIR 10
Match: AT1G05310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 421.8 bits (1083), Expect = 4.1e-118
Identity = 193/257 (75.10%), Postives = 224/257 (87.16%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 60
           ++PK+KPN+T QGQ +  TAI WNDTA S++GTFY  +VQVF S F+AKNISFMNVAPIP
Sbjct: 133 VIPKTKPNITLQGQGFDITAIAWNDTAYSANGTFYCATVQVFGSQFVAKNISFMNVAPIP 192

Query: 61  GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 120
            PGDVGAQAVAIRI GD++AF  CGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGN +S
Sbjct: 193 KPGDVGAQAVAIRIAGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKS 252

Query: 121 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 180
            Y+ C++ISMAN +  GSK VNGAVTA+GR+S DENSGF+FVNC+IGGTG VWLGRAWRP
Sbjct: 253 LYQDCRIISMANQLSPGSKAVNGAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRP 312

Query: 181 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQ 240
           +SRVVF +T MTD+IAPEGWN+FNDP+RD TIFYGEYNCSG GA+ S RAP+VQ+LN+TQ
Sbjct: 313 YSRVVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQ 372

Query: 241 VSPFLNLSFIDADQWLQ 258
           V+  +N SFID DQWLQ
Sbjct: 373 VALLINTSFIDGDQWLQ 389

[Close]

BLAST of HG10002553 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 292.7 bits (748), Expect = 2.9e-79
Identity = 139/256 (54.30%), Postives = 185/256 (72.27%), Query Frame = 0


Query: 2   VPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIPG 61
           V ++K N+  QG+ Y  T+I WNDTA S+  T  S S  VF++NF A NISF N AP P 
Sbjct: 134 VNENKTNLVIQGRGYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPD 193

Query: 62  PGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSF 121
           PG+  AQAVA+RI GDQAAF+ CGF+GAQDTL DD+GRH+FK+C+IQGSIDFIFGNGRS 
Sbjct: 194 PGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSL 253

Query: 122 YESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRPF 181
           Y+ C + S+A     G   V G++TA GR S DE SGF+FVNC I G+G + LGRAW  +
Sbjct: 254 YQDCTINSIAKGNTSG---VTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAY 313

Query: 182 SRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQV 241
           + VVF+NT M+ II PEGWN++ D  +++T+ +GE+ C G GA+   R  F ++L D++ 
Sbjct: 314 ATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEA 373

Query: 242 SPFLNLSFIDADQWLQ 258
           S F+++SFID D+WL+
Sbjct: 374 SSFIDVSFIDGDEWLR 386

[Close]

BLAST of HG10002553 vs. TAIR 10
Match: AT2G36700.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 290.0 bits (741), Expect = 1.9e-78
Identity = 133/256 (51.95%), Postives = 180/256 (70.31%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIP 60
           +V ++K N+  QG  Y+ T+I WN+T  SS+GTF S SV VF   F A NISF N AP P
Sbjct: 84  IVHENKNNLVVQGMGYSRTSIEWNNTTASSNGTFSSFSVAVFGEKFTAYNISFKNTAPAP 143

Query: 61  GPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRS 120
            PG V AQAVA+++ GD+AAF+ CGF+G QDTL D  GRH+FK C+I+GSIDFIFGNGRS
Sbjct: 144 NPGAVDAQAVALKVVGDKAAFYGCGFYGNQDTLLDQEGRHFFKGCFIEGSIDFIFGNGRS 203

Query: 121 FYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLGRAWRP 180
            YE C L S+A       +   G +TA+G+ +  + +GF FVNC I G+ RVWLGRAWRP
Sbjct: 204 LYEDCTLHSIA------KENTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRP 263

Query: 181 FSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQRLNDTQ 240
           ++RV+F+ T M+ +++ +GWND  DP   +T++YGE+ C G GAN S R  + + L+D +
Sbjct: 264 YARVIFSKTYMSRVVSLDGWNDMGDPKTQRTVYYGEHRCYGPGANHSKRVTYAKLLSDVE 323

Query: 241 VSPFLNLSFIDADQWL 257
            +PF N+SFID ++WL
Sbjct: 324 AAPFTNISFIDGEEWL 333

[Close]

BLAST of HG10002553 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 270.0 bits (689), Expect = 2.0e-72
Identity = 128/262 (48.85%), Postives = 173/262 (66.03%), Query Frame = 0


Query: 2   VPKSKPNVTFQGQSYTTTAIVWNDTANSSH------GTFYSGSVQVFSSNFIAKNISFMN 61
           +P  K  +T +G+    T + W DTA +        GT+ S S  V S  F+AKNI+F N
Sbjct: 129 IPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRN 188

Query: 62  VAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIF 121
             P+P PG VG QAVA+R+  D AAF+ C   GAQDTL+D  GRHY+KDCYI+GS+DFIF
Sbjct: 189 TTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIF 248

Query: 122 GNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWLG 181
           GN  S YE C + ++A+ +        GAVTA GR+S  E++GF+FV C + GTG ++LG
Sbjct: 249 GNALSLYEGCHVHAIADKL--------GAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLG 308

Query: 182 RAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSGTGANTSSRAPFVQR 241
           RAW PFSRVVFA T M +II P GW ++ DP+R+ T+FYG+Y C+G GAN   R  + + 
Sbjct: 309 RAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSREMTVFYGQYKCTGAGANYGGRVAWARE 368

Query: 242 LNDTQVSPFLNLSFIDADQWLQ 258
           L D +  PFL+L+FID  +W++
Sbjct: 369 LTDEEAKPFLSLTFIDGSEWIK 382

[Close]

BLAST of HG10002553 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 240.0 bits (611), Expect = 2.2e-63
Identity = 123/265 (46.42%), Postives = 173/265 (65.28%), Query Frame = 0


Query: 1   MVPKSKPNVTFQGQSYTTTAIVWNDTAN--SSHG----TFYSGSVQVFSSNFIAKNISFM 60
           +VP +KP +TF+G     TAI W+D A+   ++G    T+ + SV V+++ F A+NISF 
Sbjct: 103 VVPATKPYITFKGAGRDVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFT 162

Query: 61  NVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFI 120
           N AP P PG  G QAVA RI GD+A F  CGF+GAQDTL DD GRHYFK+CYI+GSIDFI
Sbjct: 163 NTAPAPLPGMQGWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFI 222

Query: 121 FGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNCSIGGTGRVWL 180
           FGNGRS Y+ C+L S+A      S+F  G++ AHGR   +E +GFAFV C + GTG +++
Sbjct: 223 FGNGRSMYKDCELHSIA------SRF--GSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYV 282

Query: 181 GRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPA-RDQTIFYGEYNCSGTGANTSSRAPFV 240
           GRA   +SR+V+A T    ++A  GW+D++  + + +T F+G YNC G GA  +    + 
Sbjct: 283 GRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWA 342

Query: 241 QRLNDTQVSPFLNLSFIDADQWLQP 259
           + L+     PF+  SF++   W+ P
Sbjct: 343 RALDYESAHPFIAKSFVNGRHWIAP 359

The following BLAST results are available for this feature:

BLAST of HG10002553 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008451885.1	5.1e-147	95.80	PREDICTED: probable pectinesterase 8 [Cucumis melo]	[more]
XP_038903629.1	8.7e-147	96.18	probable pectinesterase 8 [Benincasa hispida]	[more]
XP_004147773.1	3.7e-145	95.04	probable pectinesterase 8 [Cucumis sativus] >KGN53483.1 hypothetical protein Csa...	[more]
XP_022931352.1	1.4e-144	95.37	probable pectinesterase 8 [Cucurbita moschata]	[more]
KAG7015100.1	4.1e-144	94.98	putative pectinesterase 8, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of HG10002553 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23038	5.7e-117	75.10	Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2	[more]
Q9ZQA3	4.0e-78	54.30	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]
Q9ZQA4	2.6e-77	51.95	Putative pectinesterase 14 OS=Arabidopsis thaliana OX=3702 GN=PME14 PE=2 SV=1	[more]
Q8VYZ3	2.8e-71	48.85	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q8LPF3	3.1e-62	46.42	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]

BLAST of HG10002553 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BRY7	2.5e-147	95.80	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493043 PE=4 SV=1	[more]
A0A0A0KVA9	1.8e-145	95.04	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G056710 PE=4 SV=1	[more]
A0A6J1ETE2	6.8e-145	95.37	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437565 PE=4 SV=1	[more]
A0A6J1J3X1	2.6e-144	94.98	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483170 PE=4 SV=1	[more]
A0A6J1C3U9	1.3e-143	94.59	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008131 PE=4 SV=1	[more]

BLAST of HG10002553 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05310.1	4.1e-118	75.10	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	2.9e-79	54.30	Pectin lyase-like superfamily protein	[more]
AT2G36700.1	1.9e-78	51.95	Pectin lyase-like superfamily protein	[more]
AT5G19730.1	2.0e-72	48.85	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	2.2e-63	46.42	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..261 e-value: 1.5E-84 score: 286.1
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 2..252 e-value: 3.4E-56 score: 190.2
None	No IPR available	PANTHER	PTHR31321:SF33	PECTINESTERASE 8-RELATED	coord: 1..260
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 1..260
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 107..116
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 2..259

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10002553.1	HG10002553.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity