HG10002550 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10002550
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationChr11: 8150384 .. 8154752 (-)
RNA-Seq ExpressionHG10002550
SyntenyHG10002550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCTACATTCATCCAAAAACCTAGGCGACGGAGAACCACCAATCAGAGTCCAAAAATTAGTCGAAACACAGCCGTCTCAGGTTCCGCCCCAGTACATTCAGCCGCCGCATCACCGTCCCTCCGACACTAACAATTCCACCGACCTCAGAATTCCAGTGATCGATCTTTTTGGATTCGATCCGGCCCACAGACAAGCTGTTCGAGCCGACATTGGCCGAGAATCGAGCGAATGGGGAGCTTTTCAAGTCATCAACCATGGAATTCCTATATCATTGCTGAACCAGATGAGGACTGCGGCTCTCTCTTTCTTCAGAGATTGTCCCATCTCCGATAAGCTCGCCTACGCTTGCGATAAATCCTTCGCTTCCGAGGGTTATGGCACACAAATGCTCCTCGATAAAGGAGTCGACTCGCAATCTTCGGTGCTTGACTGGAGAGATTACTTCGATCACCATACGCTGCCGCTTTCACGCCGCAATCCTTCTAATTGGCCTCATTTTCCTTCCAATTACAGGGAGCTTATGGCACAGTACAGTGATTGCATGAATTTGCTTGCCCAGCGTCTCCTAGCCCTTATCTCTGAAAGTATTGGGCTGCCTTCGTCTTGGATGGAGGACGCCATCACTGGTAGGGGTGCGGAAGAAGGTTTTTATCAGAATATTACTGTCAGTTATTACCCTCCATGTCCTCAGCCTGACTTGACGCTTGGTCTGCAAACCCATTCCGACATTGGCGCCATCACTCTGCTAATACAAGATGATGTCGCCGGCCTTCAGGTTCTCAGTGGGCAAGGTGATTGGGTCATGGTTGAGCCTCTGTCTCCTGATGCCATCGTCATCATTTTAGCTGACCAAACTGAGGTTAGTTTATGCCTCCATTTCTATTTTTTTCTCCTCTCTTTGACCAAACTACACGATTCATACTACTTTATACAGAGAGTAAAGGTCTAAGATACCGGTAGGACTCTTGGAGGCTGGGACTGATGCTTGTCGTTATTTAGATAGTCAGATTACAGGGTTATAAATAAGGAGGACTTGGAGATATGAGGAGGGGAGGCTAATATTTGGACAAAATATTCGATAGAAAAGGATTGGTTGATATTAGGTGACATTGGTCAATCTCAAAACCAATTGCTTGACAAGGAGAGTAATAGTTCATGTATCTTATAAAGATTGGGAGGTTGCTTAATATTTCCAATGTCGGATCCTCAACATGCCTCTCAGGATGGTGCTTCTTTGAGTTTACCATTCTTGGATTGGATCCCAATTTTTGTTTTTGGACTCAATACCCGTTTGGGCTTCATGAGTTATGAGATAACATGGAGTTCTATCTCAAAACTAATTAGCAATTGGAGGAGTAGCCCATGTATTTTATAAACATTGTGAAAGTCTCTTGATTTTTCAATGTGGATCCTTAACCGTTGAAAAGCTATCACTCACTTTATTAATCTGCTAGTAATTTTCTGCTTGATAAATTGGGTTCCTACACCATAACACCATCAATTTCAAGCTTCAAGCTTCAAGCTTTGAAACAAGATCAATGTTTGCGGGGGCAATAATAGACCATCCATACATGAGGAAGAAGAATAGCTTATAGCTCTACGCAAGATGACGGTGGGGTAATCGTCCTATAAACTCTGGGCACTTGAGTCTATGGTGGAGGTCAGTTCAGTTATTTGGTGTAGGAGTGTTTTTATCTTAAAACGTCATTTTTTCTCTTCACGTCCAAGCACAAGGAGGTGGGGGAGTTTGCAGAAATTTAGAAATATTCTGCTAGGGAAAAACTTGGATATCTCAAGTTCTCTGAGAAGTTGATTTCTTAGAGGTTGTAATGTGATCCTTGCATGCTAGAAGTCGTTGGGTACTTATTCTCTTTTCTGATATCATCTTCCTGTATTTGAAAAGCCATTAAGCGGCAGTCAAAATGATGCTGATTGTTTAGGAGCCCATGTTTAGTTCTATGGATGAGAAGCTGTGCACACCTTGGCAGTTAATACTTTGTCACAGGGCAATTAACTGCCAATGATTAGAGTCATCCCATAAAGGTAAAAATTTTCCTCTGGGAAATTAGTCACAGAGCAATTAATACTTGTGACAAAGTTCAACGTCACTTTCTGTACAAGACTCTTTCCCCCCATTGTTGCTCTTTATGTATGGCTGCTTAGGAAACACCGTGCCATTTGTTCATCAACTGCAATTTTGCTGTTAGATTTTGGAAGGAAATTACTCTCCTTTGGATGGCCGACTGCCCTTCCTAATGACATTATTAATCTCCTCACATATACCTTCACTGGCCACCCTTTCACCGGTGGTAAAAGAACTATGTGGATGCACCTTATTAGGGCCTCCTAATGGAGCTTATGGACTGAACATTACAACCGCCTCTTCAATGATAAGGAAAATACTTTTGATAGGTTTTATGATCATATTATTTTTCTTGCTATGACTTGGTGCAAATGTACAACTTTCTATAGCTCTTATTTTCTATCCTCCCTTTTGACCAATTGGAGAAATCTTTTGTAACTCCTTTGGTTTCAGCTTATTGCCCTCCTTTTGTAAATTTCATGCATCAATGAAATTTGTTTCTTAAAAAAAGAAAAAAAGCAACCACCTGCTTACATGTCTTGTGAAAATAGTGTTTGTGTTTCGCTATCTATCTATAGCTGAGCTTGTGGTACGGGGGTTGCACTCCTCCACTTGGTTAAGGATTTGGCTTGTACCACTTGTTTGCTGTAATGGCTTGAACATTTTTTTGCTAAGAAATAGTCAAATAGGCTAACGGGGACCATCCTGTTCCAATGGTAGCTAAGCATTTAACTACATATAAAACAATGATGTTGATGTATGATTGTTGCATATCTCATGATGGAGCAAGATAACCCAAGTTGCTGGGCTTGATAATCCATTTTATTTGTCGTTGACAAACAGTCTGCAGGGAGCCTATTCTGTATTACCAAAATTACAACCTGGAAAATTTTCCTATTTATGAGTTTGAGTTGGGGAGTGTAGTGGAAGTGTGGAACTTGAACAATTCAACCAATTACAGAGTTAGAAATTTCCTCAATTTCAATCCAAATGTCCTCTCTGAGCTTCGTTGATTTGAGATTGTGCACAAATGATGGACCCGGCACTATTTTGTGAGTTAGGCCCCTACTTTTTGTTTGTTCTTAACATTTCAAGTTGAGCGCCTACCTATTGTTTGTTTTTCACGAAGGTAGTTGGTGCCTACCTCAATCGGTCTGATAATCCATGCTGAGACCACATAAGATAGTTTCCCCCTTCTACTCTATAGTTGGCCTAGATGTTCTTAAATCCCCCATGTTCCCAGCCTTGCAATAAAAAGGACTTTTGTCAACATTAATTTAAGTTGAATTAGAACTTTGAAGTTTCAATCATATCTGACATTTGGGATTTTTTAAAGGACCCTAGATGGAGAAAGCATGTGGTTGATAAGCTTTTTCAAGGGCAGAAGCTAGGGTTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTTTTGGGTTTAATTTTTAGGACAACAAATGCAATAGTTTAAATGTGCCTTTTAGGTTCCCATACCATGAAAGTGTTTGAAATGATCTGTCACACATGCTAAGGATATGCTGTAAAATGGCCATTGTAAACAAGAAAATATATGAAAGCTAAAAACTTGATTTACAATACCCATTAGATCAGGAACATATTTTGATCATTCCTAAATCAACCATGCCAAATGTTGTCTGTCCTCAACGGCTGGCAGTAGGAATCTTCAAAATTTGACATTTATAAACTGGAATGATAACTCACAATTTCTAGTTCATTACAAACAAAGACCTGAAGTGAGAGAGAAAGTTGCCTGTTAAACCATTTAAAATAGCTTAATCATTCACGCATGTTATAACTTCTATTTATCAACACCAGCATTTTTTTTTATTGGTGTCACTAATGTGTTTGACATTTTCGCTTCATTGATTAATGTGTTTGGCTATCTTGTTTCATGTGTGAGAAGTTGCTTCTTTTCTCTTATAACTTATTAAGCTTTTGTTTGTATAATCAACTAATCAAGTCAATTAGGGACTTCAATAGCACACTATTGCATGCAGATTCTAACTAATGGGAAGTGCAAGAGTGCGGAACATAGGGTGATTACAAATTCAAGCAGGGCAAGACTCTCAATTTCTGCTTTTCACGATCCACCGAAGGCAGTAAAAATATCGCCTGCTGCAGAGCTTGTTAGTGAGTCTTCCCCCCTTAGATACCGCGAAGTTATTTATGGAGACTATACATCATCATGGTACTCGAACGGCCCTGAAGGAAGAAGAAATCTGGATGCAGTCAAGCTTCACAACTAG

mRNA sequence

ATGGCGCTACATTCATCCAAAAACCTAGGCGACGGAGAACCACCAATCAGAGTCCAAAAATTAGTCGAAACACAGCCGTCTCAGGTTCCGCCCCAGTACATTCAGCCGCCGCATCACCGTCCCTCCGACACTAACAATTCCACCGACCTCAGAATTCCAGTGATCGATCTTTTTGGATTCGATCCGGCCCACAGACAAGCTGTTCGAGCCGACATTGGCCGAGAATCGAGCGAATGGGGAGCTTTTCAAGTCATCAACCATGGAATTCCTATATCATTGCTGAACCAGATGAGGACTGCGGCTCTCTCTTTCTTCAGAGATTGTCCCATCTCCGATAAGCTCGCCTACGCTTGCGATAAATCCTTCGCTTCCGAGGGTTATGGCACACAAATGCTCCTCGATAAAGGAGTCGACTCGCAATCTTCGGTGCTTGACTGGAGAGATTACTTCGATCACCATACGCTGCCGCTTTCACGCCGCAATCCTTCTAATTGGCCTCATTTTCCTTCCAATTACAGGGAGCTTATGGCACAGTACAGTGATTGCATGAATTTGCTTGCCCAGCGTCTCCTAGCCCTTATCTCTGAAAGTATTGGGCTGCCTTCGTCTTGGATGGAGGACGCCATCACTGGTAGGGGTGCGGAAGAAGGTTTTTATCAGAATATTACTGTCAGTTATTACCCTCCATGTCCTCAGCCTGACTTGACGCTTGGTCTGCAAACCCATTCCGACATTGGCGCCATCACTCTGCTAATACAAGATGATGTCGCCGGCCTTCAGGTTCTCAGTGGGCAAGGTGATTGGGTCATGGTTGAGCCTCTGTCTCCTGATGCCATCGTCATCATTTTAGCTGACCAAACTGAGATTCTAACTAATGGGAAGTGCAAGAGTGCGGAACATAGGGTGATTACAAATTCAAGCAGGGCAAGACTCTCAATTTCTGCTTTTCACGATCCACCGAAGGCAGTAAAAATATCGCCTGCTGCAGAGCTTGTTAGTGAGTCTTCCCCCCTTAGATACCGCGAAGTTATTTATGGAGACTATACATCATCATGGTACTCGAACGGCCCTGAAGGAAGAAGAAATCTGGATGCAGTCAAGCTTCACAACTAG

Coding sequence (CDS)

ATGGCGCTACATTCATCCAAAAACCTAGGCGACGGAGAACCACCAATCAGAGTCCAAAAATTAGTCGAAACACAGCCGTCTCAGGTTCCGCCCCAGTACATTCAGCCGCCGCATCACCGTCCCTCCGACACTAACAATTCCACCGACCTCAGAATTCCAGTGATCGATCTTTTTGGATTCGATCCGGCCCACAGACAAGCTGTTCGAGCCGACATTGGCCGAGAATCGAGCGAATGGGGAGCTTTTCAAGTCATCAACCATGGAATTCCTATATCATTGCTGAACCAGATGAGGACTGCGGCTCTCTCTTTCTTCAGAGATTGTCCCATCTCCGATAAGCTCGCCTACGCTTGCGATAAATCCTTCGCTTCCGAGGGTTATGGCACACAAATGCTCCTCGATAAAGGAGTCGACTCGCAATCTTCGGTGCTTGACTGGAGAGATTACTTCGATCACCATACGCTGCCGCTTTCACGCCGCAATCCTTCTAATTGGCCTCATTTTCCTTCCAATTACAGGGAGCTTATGGCACAGTACAGTGATTGCATGAATTTGCTTGCCCAGCGTCTCCTAGCCCTTATCTCTGAAAGTATTGGGCTGCCTTCGTCTTGGATGGAGGACGCCATCACTGGTAGGGGTGCGGAAGAAGGTTTTTATCAGAATATTACTGTCAGTTATTACCCTCCATGTCCTCAGCCTGACTTGACGCTTGGTCTGCAAACCCATTCCGACATTGGCGCCATCACTCTGCTAATACAAGATGATGTCGCCGGCCTTCAGGTTCTCAGTGGGCAAGGTGATTGGGTCATGGTTGAGCCTCTGTCTCCTGATGCCATCGTCATCATTTTAGCTGACCAAACTGAGATTCTAACTAATGGGAAGTGCAAGAGTGCGGAACATAGGGTGATTACAAATTCAAGCAGGGCAAGACTCTCAATTTCTGCTTTTCACGATCCACCGAAGGCAGTAAAAATATCGCCTGCTGCAGAGCTTGTTAGTGAGTCTTCCCCCCTTAGATACCGCGAAGTTATTTATGGAGACTATACATCATCATGGTACTCGAACGGCCCTGAAGGAAGAAGAAATCTGGATGCAGTCAAGCTTCACAACTAG

Protein sequence

MALHSSKNLGDGEPPIRVQKLVETQPSQVPPQYIQPPHHRPSDTNNSTDLRIPVIDLFGFDPAHRQAVRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACDKSFASEGYGTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQYSDCMNLLAQRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGLQTHSDIGAITLLIQDDVAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAEHRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEGRRNLDAVKLHN
Homology
BLAST of HG10002550 vs. NCBI nr
Match: XP_038895425.1 (probable 2-oxoglutarate-dependent dioxygenase ANS [Benincasa hispida] >XP_038895433.1 probable 2-oxoglutarate-dependent dioxygenase ANS [Benincasa hispida])

HSP 1 Score: 699.5 bits (1804), Expect = 1.5e-197
Identity = 344/371 (92.72%), Postives = 354/371 (95.42%), Query Frame = 0

Query: 1   MALHSSKNLGDGEPPIRVQKLVETQPSQVPPQYIQPPHHRPSDTNNSTDLRIPVIDLFGF 60
           MAL+SS +LGDGEP IRVQKLVETQPS+VPPQYIQPPHHRPSDTN STDL+IPVIDLFGF
Sbjct: 1   MALNSSNSLGDGEPVIRVQKLVETQPSRVPPQYIQPPHHRPSDTNKSTDLKIPVIDLFGF 60

Query: 61  DPAHRQAVRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACD- 120
           D AHRQAVRA IGRESSEWGAFQVINHGIP+SLLNQMRTAALSFFRDCP+SDKLAYACD 
Sbjct: 61  DTAHRQAVRAAIGRESSEWGAFQVINHGIPLSLLNQMRTAALSFFRDCPVSDKLAYACDP 120

Query: 121 KSFASEGYGTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQY 180
           KSFASEGYGTQMLLDK VDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQY
Sbjct: 121 KSFASEGYGTQMLLDKDVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQY 180

Query: 181 SDCMNLLAQRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGL 240
           SD M LLAQRLLALISE+IGLPSSWMEDAITG GAEEGFYQNITVSYYPPCPQPDLTLGL
Sbjct: 181 SDWMKLLAQRLLALISETIGLPSSWMEDAITGMGAEEGFYQNITVSYYPPCPQPDLTLGL 240

Query: 241 QTHSDIGAITLLIQDDVAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAE 300
           Q+HSDIGAITLLIQDDV GLQVLS QGDWV V+P  PDAIV+ILADQTEILTNGKCKSAE
Sbjct: 241 QSHSDIGAITLLIQDDVGGLQVLSAQGDWVTVQP-DPDAIVVILADQTEILTNGKCKSAE 300

Query: 301 HRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEG 360
           HRVITNS RARLSISAFHDPPKAVKI PAAELVSESSPLRYREVIYGDYTSSWYSNGPEG
Sbjct: 301 HRVITNSRRARLSISAFHDPPKAVKILPAAELVSESSPLRYREVIYGDYTSSWYSNGPEG 360

Query: 361 RRNLDAVKLHN 371
           RRNLDAVKLHN
Sbjct: 361 RRNLDAVKLHN 370

BLAST of HG10002550 vs. NCBI nr
Match: XP_008451879.1 (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase 5 isoform X1 [Cucumis melo])

HSP 1 Score: 676.8 bits (1745), Expect = 1.1e-190
Identity = 335/371 (90.30%), Postives = 346/371 (93.26%), Query Frame = 0

Query: 1   MALHSSKNLGDGEPPIRVQKLVETQPSQVPPQYIQPPHHRPSDTNNSTDLRIPVIDLFGF 60
           MAL SS      EP IRVQKL+ET PSQVPPQYIQPPHHRPS TNNST L IPVIDLFGF
Sbjct: 1   MALQSS------EPQIRVQKLLETHPSQVPPQYIQPPHHRPSHTNNSTHLSIPVIDLFGF 60

Query: 61  DPAHRQAVRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACD- 120
           DP+HR AVRA IGRES EWGAFQVINHGIPISLLN+MR AALSFFRD P+SDKLAYACD 
Sbjct: 61  DPSHRPAVRAAIGRESGEWGAFQVINHGIPISLLNRMRAAALSFFRDYPVSDKLAYACDP 120

Query: 121 KSFASEGYGTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQY 180
            SFASEGYGTQMLLDK V SQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPS+YRELMAQY
Sbjct: 121 NSFASEGYGTQMLLDKHVHSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSDYRELMAQY 180

Query: 181 SDCMNLLAQRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGL 240
           SDCM LL+QRLLALISESIGLPSSWMEDAITG GAEEGFYQNITVSYYPPCPQPDLTLGL
Sbjct: 181 SDCMKLLSQRLLALISESIGLPSSWMEDAITGMGAEEGFYQNITVSYYPPCPQPDLTLGL 240

Query: 241 QTHSDIGAITLLIQDDVAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAE 300
           Q+HSDIGAITLLIQDDV+GLQVLSGQGDWV V PLSPDAIV+ILADQTEILTNGKCKSAE
Sbjct: 241 QSHSDIGAITLLIQDDVSGLQVLSGQGDWVTVHPLSPDAIVVILADQTEILTNGKCKSAE 300

Query: 301 HRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEG 360
           HRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSP+RYREVIYGDYTSSWYSNGPEG
Sbjct: 301 HRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSPVRYREVIYGDYTSSWYSNGPEG 360

Query: 361 RRNLDAVKLHN 371
           RRNLDAVKL+N
Sbjct: 361 RRNLDAVKLYN 365

BLAST of HG10002550 vs. NCBI nr
Match: KAG7015103.1 (putative 2-oxoglutarate-dependent dioxygenase ANS [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 668.3 bits (1723), Expect = 3.8e-188
Identity = 326/371 (87.87%), Postives = 341/371 (91.91%), Query Frame = 0

Query: 1   MALHSSKNLGDGEPPIRVQKLVETQPSQVPPQYIQPPHHRPSDTNNSTDLRIPVIDLFGF 60
           MALHSS++L  GE  IRVQ LVE QPSQVPPQYIQPPHHRPS T+ STDLRIPVIDLFGF
Sbjct: 1   MALHSSESLAGGESVIRVQNLVEAQPSQVPPQYIQPPHHRPSATSTSTDLRIPVIDLFGF 60

Query: 61  DPAHRQAVRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACD- 120
           D  HR AVRA +GR SSEWGAF VINHGIP SLL QMRTAALSFFRDCP+SDKLAYACD 
Sbjct: 61  DSDHRDAVRAAVGRASSEWGAFHVINHGIPSSLLRQMRTAALSFFRDCPVSDKLAYACDP 120

Query: 121 KSFASEGYGTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQY 180
           KSFASEGYGTQML+DK VDSQ S LDWRDYFDHHTLPLSRRN SNWP+FPS YRELMAQY
Sbjct: 121 KSFASEGYGTQMLVDKEVDSQDSALDWRDYFDHHTLPLSRRNLSNWPYFPSGYRELMAQY 180

Query: 181 SDCMNLLAQRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGL 240
           SDCM LLAQRLLA+ISESIGLPSSWMEDAITG G EE FYQNITVSYYPPCPQP LTLGL
Sbjct: 181 SDCMKLLAQRLLAVISESIGLPSSWMEDAITGMGGEENFYQNITVSYYPPCPQPGLTLGL 240

Query: 241 QTHSDIGAITLLIQDDVAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAE 300
           QTHSDIGAITLLIQDDV GLQVLSGQGDWV V+PLSPDAIV+ILADQTEILTNGKCKSAE
Sbjct: 241 QTHSDIGAITLLIQDDVEGLQVLSGQGDWVTVQPLSPDAIVVILADQTEILTNGKCKSAE 300

Query: 301 HRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEG 360
           HRV TNSSRARLSISAFHDPPK+VKISPAAELVSESSPLRYR+VI+GDYTSSWYSNGP+G
Sbjct: 301 HRVTTNSSRARLSISAFHDPPKSVKISPAAELVSESSPLRYRDVIFGDYTSSWYSNGPQG 360

Query: 361 RRNLDAVKLHN 371
           RRNLDA+KLHN
Sbjct: 361 RRNLDAIKLHN 371

BLAST of HG10002550 vs. NCBI nr
Match: XP_022931353.1 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X1 [Cucurbita moschata] >XP_022931354.1 probable 2-oxoglutarate-dependent dioxygenase ANS isoform X1 [Cucurbita moschata] >XP_022931355.1 probable 2-oxoglutarate-dependent dioxygenase ANS isoform X1 [Cucurbita moschata] >XP_022931356.1 probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 [Cucurbita moschata])

HSP 1 Score: 668.3 bits (1723), Expect = 3.8e-188
Identity = 326/371 (87.87%), Postives = 341/371 (91.91%), Query Frame = 0

Query: 1   MALHSSKNLGDGEPPIRVQKLVETQPSQVPPQYIQPPHHRPSDTNNSTDLRIPVIDLFGF 60
           MALHSS++L  GE  IRVQ LVE QPSQVPPQYIQPPHHRPS T+ STDLRIPVIDLFGF
Sbjct: 1   MALHSSESLAGGESVIRVQNLVEAQPSQVPPQYIQPPHHRPSATSTSTDLRIPVIDLFGF 60

Query: 61  DPAHRQAVRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACD- 120
           D  HR AVRA +GR SSEWGAF VINHGIP SLL QMRTAALSFFRDCP+SDKLAYACD 
Sbjct: 61  DSDHRDAVRAAVGRASSEWGAFHVINHGIPSSLLTQMRTAALSFFRDCPVSDKLAYACDP 120

Query: 121 KSFASEGYGTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQY 180
           KSFASEGYGTQML+DK VDSQ S LDWRDYFDHHTLPLSRRN SNWP+FPS YRELMAQY
Sbjct: 121 KSFASEGYGTQMLVDKEVDSQDSALDWRDYFDHHTLPLSRRNLSNWPYFPSGYRELMAQY 180

Query: 181 SDCMNLLAQRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGL 240
           SDCM LLAQRLLA+ISES+GLPSSWMEDAITG G EE FYQNITVSYYPPCPQP LTLGL
Sbjct: 181 SDCMKLLAQRLLAVISESMGLPSSWMEDAITGMGGEENFYQNITVSYYPPCPQPGLTLGL 240

Query: 241 QTHSDIGAITLLIQDDVAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAE 300
           QTHSDIGAITLLIQDDV GLQVLSGQGDWV V+PLSPDAIV+ILADQTEILTNGKCKSAE
Sbjct: 241 QTHSDIGAITLLIQDDVEGLQVLSGQGDWVTVQPLSPDAIVVILADQTEILTNGKCKSAE 300

Query: 301 HRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEG 360
           HRV TNSSRARLSISAFHDPPK+VKISPAAELVSESSPLRYREVI+GDYTSSWYSNGP+G
Sbjct: 301 HRVTTNSSRARLSISAFHDPPKSVKISPAAELVSESSPLRYREVIFGDYTSSWYSNGPQG 360

Query: 361 RRNLDAVKLHN 371
           RRNLDA+KLHN
Sbjct: 361 RRNLDAIKLHN 371

BLAST of HG10002550 vs. NCBI nr
Match: XP_022985093.1 (probable 2-oxoglutarate-dependent dioxygenase ANS [Cucurbita maxima] >XP_022985094.1 probable 2-oxoglutarate-dependent dioxygenase ANS [Cucurbita maxima] >XP_022985095.1 probable 2-oxoglutarate-dependent dioxygenase ANS [Cucurbita maxima])

HSP 1 Score: 667.2 bits (1720), Expect = 8.5e-188
Identity = 324/371 (87.33%), Postives = 342/371 (92.18%), Query Frame = 0

Query: 1   MALHSSKNLGDGEPPIRVQKLVETQPSQVPPQYIQPPHHRPSDTNNSTDLRIPVIDLFGF 60
           MALHSS++L  GE  IRVQ LVE QPSQVPPQYIQPPHHRPS T+ STDLRIPVIDLFGF
Sbjct: 1   MALHSSESLAGGESVIRVQNLVEAQPSQVPPQYIQPPHHRPSATSTSTDLRIPVIDLFGF 60

Query: 61  DPAHRQAVRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACD- 120
           DP HR AVR  +GR SSEWGAF VINHGIP SLL+QMRTAALSFFRDCP+SDKLAYACD 
Sbjct: 61  DPDHRDAVRGAVGRASSEWGAFHVINHGIPSSLLSQMRTAALSFFRDCPVSDKLAYACDP 120

Query: 121 KSFASEGYGTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQY 180
           KSFASEGYGTQML+DK V+SQ+S LDWRDYFDHHTLPLSRRN SNWPHFPS YRELMAQY
Sbjct: 121 KSFASEGYGTQMLVDKEVESQASALDWRDYFDHHTLPLSRRNLSNWPHFPSGYRELMAQY 180

Query: 181 SDCMNLLAQRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGL 240
           SDCM LLAQRLLA+ISESIGLPSSWMEDAITG G EE FYQNITVSYYPPCPQP LTLGL
Sbjct: 181 SDCMKLLAQRLLAVISESIGLPSSWMEDAITGMGGEENFYQNITVSYYPPCPQPGLTLGL 240

Query: 241 QTHSDIGAITLLIQDDVAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAE 300
           QTHSDIGAITLLIQDDV GLQVLSGQGDWV V+ LSP+AI++ILADQTEILTNGKCKSAE
Sbjct: 241 QTHSDIGAITLLIQDDVEGLQVLSGQGDWVTVQSLSPNAIIVILADQTEILTNGKCKSAE 300

Query: 301 HRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEG 360
           HRV TNSSRARLSISAFHDPPK+VKISPAAELVSESSPLRYREVI+GDYTSSWYSNGP+G
Sbjct: 301 HRVTTNSSRARLSISAFHDPPKSVKISPAAELVSESSPLRYREVIFGDYTSSWYSNGPQG 360

Query: 361 RRNLDAVKLHN 371
           RRNLDA+KLHN
Sbjct: 361 RRNLDAIKLHN 371

BLAST of HG10002550 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 8.2e-61
Identity = 138/361 (38.23%), Postives = 213/361 (59.00%), Query Frame = 0

Query: 13  EPPIRVQKLVETQPSQVPPQYIQPPHHRP--SDTNNSTDLRIPVIDLFGF--DPAHRQAV 72
           EP + VQ L +T    VP +Y++P H RP  + T +   + IPV+D+      P   + V
Sbjct: 7   EPIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPEGLRLV 66

Query: 73  RADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACDKSFASEGYG 132
           R+       EWG FQ++NHG+  SL+ ++R A   FF + P+ +K  YA +     EGYG
Sbjct: 67  RS----ACEEWGFFQMVNHGVTHSLMERVRGAWREFF-ELPLEEKRKYA-NSPDTYEGYG 126

Query: 133 TQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQYSDCMNLLAQ 192
           +++ + K      + LDW DYF  + LP S RNPS WP  P   REL+ +Y + +  L +
Sbjct: 127 SRLGVVK-----DAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCE 186

Query: 193 RLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGLQTHSDIGAI 252
           RL   +SES+GL  + +  A+ G G + G   ++  ++YP CPQP LTLGL +HSD G I
Sbjct: 187 RLTETLSESLGLKPNKLMQAL-GGGDKVG--ASLRTNFYPKCPQPQLTLGLSSHSDPGGI 246

Query: 253 TLLIQDD-VAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAEHRVITNSS 312
           T+L+ D+ VAGLQV  G G WV ++ + P+A+++ + DQ +IL+NG  KS EH+VI NS 
Sbjct: 247 TILLPDEKVAGLQVRRGDG-WVTIKSV-PNALIVNIGDQLQILSNGIYKSVEHQVIVNSG 306

Query: 313 RARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEGRRNLDAVK 369
             R+S++ F++P   + + P  ELV+ + P  Y+ + + +Y S     GP G+  +D++ 
Sbjct: 307 MERVSLAFFYNPRSDIPVGPIEELVTANRPALYKPIRFDEYRSLIRQKGPCGKNQVDSLL 351

BLAST of HG10002550 vs. ExPASy Swiss-Prot
Match: Q9SRM3 (Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 9.1e-60
Identity = 132/359 (36.77%), Postives = 203/359 (56.55%), Query Frame = 0

Query: 13  EPPIRVQKLVETQPSQVPPQYIQPPHHRPSDT-----NNSTDLRIPVIDLFGFDPAHRQA 72
           EP +RVQ L E+  + +P +YI+PP  RP  T         D+ IP+IDL      +   
Sbjct: 51  EPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNEDD 110

Query: 73  VRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACDKSFASEGY 132
            +  I     EWG FQVINHG+   L++  R    SFF + P+  K  Y+ +     EGY
Sbjct: 111 -KKRISEACREWGFFQVINHGVKPELMDAARETWKSFF-NLPVEAKEVYS-NSPRTYEGY 170

Query: 133 GTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQYSDCMNLLA 192
           G+++ ++KG     ++LDW DY+  H LPL+ ++ + WP  PSN RE+  +Y   +  L 
Sbjct: 171 GSRLGVEKG-----AILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLG 230

Query: 193 QRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGLQTHSDIGA 252
            RL+ ++S ++GL +  +++A  G    E     + V+YYP CPQP+L LGL  HSD G 
Sbjct: 231 GRLMTILSSNLGLRAEQLQEAFGG----EDVGACLRVNYYPKCPQPELALGLSPHSDPGG 290

Query: 253 ITLLIQDD-VAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAEHRVITNS 312
           +T+L+ DD V GLQV  G   W+ V PL   A ++ + DQ +IL+N K KS EHRVI NS
Sbjct: 291 MTILLPDDQVVGLQVRHGD-TWITVNPLR-HAFIVNIGDQIQILSNSKYKSVEHRVIVNS 350

Query: 313 SRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEGRRNLDA 366
            + R+S++ F++P   + I P  +LV+ + P  Y  + +  Y     + GP G+ ++++
Sbjct: 351 EKERVSLAFFYNPKSDIPIQPMQQLVTSTMPPLYPPMTFDQYRLFIRTQGPRGKSHVES 395

BLAST of HG10002550 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 4.7e-56
Identity = 127/358 (35.47%), Postives = 200/358 (55.87%), Query Frame = 0

Query: 13  EPPIRVQKLVETQPSQVPPQYIQPPHHRPSDTNNS-TDLRIPVID---LFGFDPAHRQAV 72
           EP +RVQ L E+  S +P +YI+P   RP+ T ++ T   IP+ID   LF  +      +
Sbjct: 22  EPIVRVQSLAESNLSSLPDRYIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEGLSDDVI 81

Query: 73  RADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACDKSFASEGYG 132
            A I      WG FQV+NHG+   L++  R     FF   P++ K  Y+ +     EGYG
Sbjct: 82  MARISEACRGWGFFQVVNHGVKPELMDAARENWREFFH-MPVNAKETYS-NSPRTYEGYG 141

Query: 133 TQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQYSDCMNLLAQ 192
           +++ ++KG       LDW DY+  H LP   ++ + WP FP   RE++ +Y + +  L+ 
Sbjct: 142 SRLGVEKGAS-----LDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSG 201

Query: 193 RLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGLQTHSDIGAI 252
           R++ ++S ++GL     ++A  G    E     + V+YYP CP+P+L LGL  HSD G +
Sbjct: 202 RIMRVLSTNLGLKEDKFQEAFGG----ENIGACLRVNYYPKCPRPELALGLSPHSDPGGM 261

Query: 253 TLLIQDD-VAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAEHRVITNSS 312
           T+L+ DD V GLQV      W+ V+P  P A ++ + DQ +IL+N   KS EHRVI NS 
Sbjct: 262 TILLPDDQVFGLQVRK-DDTWITVKP-HPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSD 321

Query: 313 RARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEGRRNLDA 366
           + R+S++ F++P   + I P  ELVS  +P  Y  + +  Y     + GP+G+ ++++
Sbjct: 322 KERVSLAFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYRLFIRTQGPQGKSHVES 366

BLAST of HG10002550 vs. ExPASy Swiss-Prot
Match: Q9LY48 (Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.4e-55
Identity = 127/363 (34.99%), Postives = 200/363 (55.10%), Query Frame = 0

Query: 13  EPPIRVQKLVETQPSQVPPQYIQPPHHRPSDT----NNSTDLRIPVID---LFGFDPAHR 72
           EP +RVQ L E+    +P +Y++P   RP+ T    +N     IP+ID   L+  D   +
Sbjct: 9   EPIVRVQSLSESNLGAIPNRYVKPLSQRPNITPHNKHNPQTTTIPIIDLGRLYTDDLTLQ 68

Query: 73  QAVRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACDKSFASE 132
                +I +   E G FQV+NHG+   L++Q +     FF + P+  K  +A +     E
Sbjct: 69  AKTLDEISKACRELGFFQVVNHGMSPQLMDQAKATWREFF-NLPMELKNMHA-NSPKTYE 128

Query: 133 GYGTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQYSDCMNL 192
           GYG+++ ++KG     ++LDW DY+  H  P S ++ + WP  P + RE++  Y   M  
Sbjct: 129 GYGSRLGVEKG-----AILDWSDYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMVK 188

Query: 193 LAQRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGLQTHSDI 252
           L + L+ ++S+++GL    +++A  G+    G    + V+YYP CPQP+LTLG+  HSD 
Sbjct: 189 LCENLMKILSKNLGLQEDRLQNAFGGKEESGGC---LRVNYYPKCPQPELTLGISPHSDP 248

Query: 253 GAITLLIQDD-VAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAEHRVIT 312
           G +T+L+ D+ VA LQV      W+ VEP +P A ++ + DQ ++L+N   KS EHRVI 
Sbjct: 249 GGLTILLPDEQVASLQVRGSDDAWITVEP-APHAFIVNMGDQIQMLSNSIYKSVEHRVIV 308

Query: 313 NSSRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEGRRNLD 368
           N    RLS++ F++P   V I P  ELV+  SP  Y    Y  Y     + GP  + ++D
Sbjct: 309 NPENERLSLAFFYNPKGNVPIEPLKELVTVDSPALYSSTTYDRYRQFIRTQGPRSKCHID 360

BLAST of HG10002550 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 5.9e-51
Identity = 125/337 (37.09%), Postives = 185/337 (54.90%), Query Frame = 0

Query: 29  VPPQYIQPPHHRPSDTNNSTDLRIPVIDLFGFDPAHRQAVRADIGRESSEWGAFQVINHG 88
           +P +Y++P   RP      +D RIPV+DL   D   R AV + +G      G FQV+NHG
Sbjct: 20  MPGKYVRPESQRPRLDLVVSDARIPVVDLASPD---RAAVVSAVGDACRTHGFFQVVNHG 79

Query: 89  IPISLLNQMRTAALSFFRDCPISDKLAYACDKSFASEGYGTQMLLDKGVDSQSSVLDWRD 148
           I  +L+  +      FFR  P  +K     D         T   + K      +V +WRD
Sbjct: 80  IDAALIASVMEVGREFFR-LPAEEKAKLYSDDPAKKIRLSTSFNVRK-----ETVHNWRD 139

Query: 149 YFDHHTLPLSRRNPSNWPHFPSNYRELMAQYSDCMNLLAQRLLALISESIGLPSSWMEDA 208
           Y   H  PL +  P +WP  P +++E++  Y   +  L  RL   ISES+GL   +M + 
Sbjct: 140 YLRLHCYPLHQFVP-DWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRET 199

Query: 209 ITGRGAEEGFYQNITVSYYPPCPQPDLTLGLQTHSDIGAITLLIQDD-VAGLQVLSGQGD 268
           +   G +E   Q++ V+YYP CP+P+LT GL  H+D  A+T+L+ DD VAGLQVL+  G 
Sbjct: 200 L---GEQE---QHMAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLN-DGK 259

Query: 269 WVMVEPLSPDAIVIILADQTEILTNGKCKSAEHRVITNSSRARLSISAFHDPPKAVKISP 328
           W+ V P  P A+VI + DQ + L+NGK +S  HR + NS R R+S+++F  P  +V++ P
Sbjct: 260 WIAVNP-QPGALVINIGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGP 319

Query: 329 AAELVSESSPLRYREVIYGDYTSSWYSNGPEGRRNLD 365
           A +L+++ SP  YR   Y +Y   ++S      RNLD
Sbjct: 320 AKKLITDDSPAVYRNYTYDEYYKKFWS------RNLD 332

BLAST of HG10002550 vs. ExPASy TrEMBL
Match: A0A1S3BTN2 (1-aminocyclopropane-1-carboxylate oxidase 5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493039 PE=3 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 5.2e-191
Identity = 335/371 (90.30%), Postives = 346/371 (93.26%), Query Frame = 0

Query: 1   MALHSSKNLGDGEPPIRVQKLVETQPSQVPPQYIQPPHHRPSDTNNSTDLRIPVIDLFGF 60
           MAL SS      EP IRVQKL+ET PSQVPPQYIQPPHHRPS TNNST L IPVIDLFGF
Sbjct: 1   MALQSS------EPQIRVQKLLETHPSQVPPQYIQPPHHRPSHTNNSTHLSIPVIDLFGF 60

Query: 61  DPAHRQAVRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACD- 120
           DP+HR AVRA IGRES EWGAFQVINHGIPISLLN+MR AALSFFRD P+SDKLAYACD 
Sbjct: 61  DPSHRPAVRAAIGRESGEWGAFQVINHGIPISLLNRMRAAALSFFRDYPVSDKLAYACDP 120

Query: 121 KSFASEGYGTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQY 180
            SFASEGYGTQMLLDK V SQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPS+YRELMAQY
Sbjct: 121 NSFASEGYGTQMLLDKHVHSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSDYRELMAQY 180

Query: 181 SDCMNLLAQRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGL 240
           SDCM LL+QRLLALISESIGLPSSWMEDAITG GAEEGFYQNITVSYYPPCPQPDLTLGL
Sbjct: 181 SDCMKLLSQRLLALISESIGLPSSWMEDAITGMGAEEGFYQNITVSYYPPCPQPDLTLGL 240

Query: 241 QTHSDIGAITLLIQDDVAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAE 300
           Q+HSDIGAITLLIQDDV+GLQVLSGQGDWV V PLSPDAIV+ILADQTEILTNGKCKSAE
Sbjct: 241 QSHSDIGAITLLIQDDVSGLQVLSGQGDWVTVHPLSPDAIVVILADQTEILTNGKCKSAE 300

Query: 301 HRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEG 360
           HRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSP+RYREVIYGDYTSSWYSNGPEG
Sbjct: 301 HRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSPVRYREVIYGDYTSSWYSNGPEG 360

Query: 361 RRNLDAVKLHN 371
           RRNLDAVKL+N
Sbjct: 361 RRNLDAVKLYN 365

BLAST of HG10002550 vs. ExPASy TrEMBL
Match: A0A6J1EYB1 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437566 PE=3 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 1.8e-188
Identity = 326/371 (87.87%), Postives = 341/371 (91.91%), Query Frame = 0

Query: 1   MALHSSKNLGDGEPPIRVQKLVETQPSQVPPQYIQPPHHRPSDTNNSTDLRIPVIDLFGF 60
           MALHSS++L  GE  IRVQ LVE QPSQVPPQYIQPPHHRPS T+ STDLRIPVIDLFGF
Sbjct: 1   MALHSSESLAGGESVIRVQNLVEAQPSQVPPQYIQPPHHRPSATSTSTDLRIPVIDLFGF 60

Query: 61  DPAHRQAVRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACD- 120
           D  HR AVRA +GR SSEWGAF VINHGIP SLL QMRTAALSFFRDCP+SDKLAYACD 
Sbjct: 61  DSDHRDAVRAAVGRASSEWGAFHVINHGIPSSLLTQMRTAALSFFRDCPVSDKLAYACDP 120

Query: 121 KSFASEGYGTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQY 180
           KSFASEGYGTQML+DK VDSQ S LDWRDYFDHHTLPLSRRN SNWP+FPS YRELMAQY
Sbjct: 121 KSFASEGYGTQMLVDKEVDSQDSALDWRDYFDHHTLPLSRRNLSNWPYFPSGYRELMAQY 180

Query: 181 SDCMNLLAQRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGL 240
           SDCM LLAQRLLA+ISES+GLPSSWMEDAITG G EE FYQNITVSYYPPCPQP LTLGL
Sbjct: 181 SDCMKLLAQRLLAVISESMGLPSSWMEDAITGMGGEENFYQNITVSYYPPCPQPGLTLGL 240

Query: 241 QTHSDIGAITLLIQDDVAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAE 300
           QTHSDIGAITLLIQDDV GLQVLSGQGDWV V+PLSPDAIV+ILADQTEILTNGKCKSAE
Sbjct: 241 QTHSDIGAITLLIQDDVEGLQVLSGQGDWVTVQPLSPDAIVVILADQTEILTNGKCKSAE 300

Query: 301 HRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEG 360
           HRV TNSSRARLSISAFHDPPK+VKISPAAELVSESSPLRYREVI+GDYTSSWYSNGP+G
Sbjct: 301 HRVTTNSSRARLSISAFHDPPKSVKISPAAELVSESSPLRYREVIFGDYTSSWYSNGPQG 360

Query: 361 RRNLDAVKLHN 371
           RRNLDA+KLHN
Sbjct: 361 RRNLDAIKLHN 371

BLAST of HG10002550 vs. ExPASy TrEMBL
Match: A0A6J1JAE5 (probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita maxima OX=3661 GN=LOC111483171 PE=3 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 4.1e-188
Identity = 324/371 (87.33%), Postives = 342/371 (92.18%), Query Frame = 0

Query: 1   MALHSSKNLGDGEPPIRVQKLVETQPSQVPPQYIQPPHHRPSDTNNSTDLRIPVIDLFGF 60
           MALHSS++L  GE  IRVQ LVE QPSQVPPQYIQPPHHRPS T+ STDLRIPVIDLFGF
Sbjct: 1   MALHSSESLAGGESVIRVQNLVEAQPSQVPPQYIQPPHHRPSATSTSTDLRIPVIDLFGF 60

Query: 61  DPAHRQAVRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACD- 120
           DP HR AVR  +GR SSEWGAF VINHGIP SLL+QMRTAALSFFRDCP+SDKLAYACD 
Sbjct: 61  DPDHRDAVRGAVGRASSEWGAFHVINHGIPSSLLSQMRTAALSFFRDCPVSDKLAYACDP 120

Query: 121 KSFASEGYGTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQY 180
           KSFASEGYGTQML+DK V+SQ+S LDWRDYFDHHTLPLSRRN SNWPHFPS YRELMAQY
Sbjct: 121 KSFASEGYGTQMLVDKEVESQASALDWRDYFDHHTLPLSRRNLSNWPHFPSGYRELMAQY 180

Query: 181 SDCMNLLAQRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGL 240
           SDCM LLAQRLLA+ISESIGLPSSWMEDAITG G EE FYQNITVSYYPPCPQP LTLGL
Sbjct: 181 SDCMKLLAQRLLAVISESIGLPSSWMEDAITGMGGEENFYQNITVSYYPPCPQPGLTLGL 240

Query: 241 QTHSDIGAITLLIQDDVAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAE 300
           QTHSDIGAITLLIQDDV GLQVLSGQGDWV V+ LSP+AI++ILADQTEILTNGKCKSAE
Sbjct: 241 QTHSDIGAITLLIQDDVEGLQVLSGQGDWVTVQSLSPNAIIVILADQTEILTNGKCKSAE 300

Query: 301 HRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEG 360
           HRV TNSSRARLSISAFHDPPK+VKISPAAELVSESSPLRYREVI+GDYTSSWYSNGP+G
Sbjct: 301 HRVTTNSSRARLSISAFHDPPKSVKISPAAELVSESSPLRYREVIFGDYTSSWYSNGPQG 360

Query: 361 RRNLDAVKLHN 371
           RRNLDA+KLHN
Sbjct: 361 RRNLDAIKLHN 371

BLAST of HG10002550 vs. ExPASy TrEMBL
Match: A0A0A0L036 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G056660 PE=3 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 7.0e-188
Identity = 329/371 (88.68%), Postives = 341/371 (91.91%), Query Frame = 0

Query: 1   MALHSSKNLGDGEPPIRVQKLVETQPSQVPPQYIQPPHHRPSDTNNSTDLRIPVIDLFGF 60
           MAL SS      +P  RVQKL ET PSQVPPQYIQPPHHRPS  NNST L IPVIDLFGF
Sbjct: 1   MALQSS------QPLTRVQKLAETHPSQVPPQYIQPPHHRPSHPNNSTHLSIPVIDLFGF 60

Query: 61  DPAHRQAVRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACD- 120
           DP+HR AV A IGRES+EWGAFQVINHGIP+SLLNQMR AALSFFRD P+SDKLAYACD 
Sbjct: 61  DPSHRPAVLAAIGRESTEWGAFQVINHGIPVSLLNQMRAAALSFFRDYPVSDKLAYACDP 120

Query: 121 KSFASEGYGTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQY 180
            SFASEGYGTQMLLD+ V SQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPS+YRELMAQY
Sbjct: 121 NSFASEGYGTQMLLDQHVRSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSHYRELMAQY 180

Query: 181 SDCMNLLAQRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGL 240
           SDCM LL+QRLLALISE+IGLPSSWMEDAITG GAEEGFYQNITVSYYPPCPQPDLTLGL
Sbjct: 181 SDCMKLLSQRLLALISETIGLPSSWMEDAITGMGAEEGFYQNITVSYYPPCPQPDLTLGL 240

Query: 241 QTHSDIGAITLLIQDDVAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAE 300
           Q+HSDIGAITLLIQDDV+GLQVLSGQGDWV   PLSPDAIV+ILADQTEILTNGKCKSAE
Sbjct: 241 QSHSDIGAITLLIQDDVSGLQVLSGQGDWVTAHPLSPDAIVVILADQTEILTNGKCKSAE 300

Query: 301 HRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEG 360
           HRVITNSSRARLSISAFHDPPK VKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEG
Sbjct: 301 HRVITNSSRARLSISAFHDPPKTVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEG 360

Query: 361 RRNLDAVKLHN 371
           RRNLDAVKLHN
Sbjct: 361 RRNLDAVKLHN 365

BLAST of HG10002550 vs. ExPASy TrEMBL
Match: A0A6J1C408 (probable 2-oxoglutarate-dependent dioxygenase ANS OS=Momordica charantia OX=3673 GN=LOC111008184 PE=3 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 1.6e-176
Identity = 306/371 (82.48%), Postives = 326/371 (87.87%), Query Frame = 0

Query: 1   MALHSSKNLGDGEPPIRVQKLVETQPSQVPPQYIQPPHHRPSDTNNSTDLRIPVIDLFGF 60
           MAL SS + G G    RVQKL ET+PSQVPPQYIQPPHHRPS  NNS+D+RIPVIDLF F
Sbjct: 1   MALDSSTSPGGGGAATRVQKLAETEPSQVPPQYIQPPHHRPSANNNSSDVRIPVIDLFNF 60

Query: 61  DPAHRQAVRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACD- 120
           DP HR +VR  IGR SSEWGAFQVINHGIP SLLNQ RTAALSFFRDCP+SDKL YACD 
Sbjct: 61  DPEHRDSVRDAIGRASSEWGAFQVINHGIPSSLLNQTRTAALSFFRDCPVSDKLVYACDP 120

Query: 121 KSFASEGYGTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQY 180
            +FASEGYGTQML+ K  + QSSVLDWRDYFDHHTLPLSRRN SNWPHFP NYRE+MAQY
Sbjct: 121 NAFASEGYGTQMLVAKEGEPQSSVLDWRDYFDHHTLPLSRRNSSNWPHFPPNYREVMAQY 180

Query: 181 SDCMNLLAQRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGL 240
           SDC+ LLAQR+LA+ISESIGLPSSWMEDAITG G EE  YQNITVSYYPPCPQPDLTLGL
Sbjct: 181 SDCVKLLAQRILAVISESIGLPSSWMEDAITGMGEEEDIYQNITVSYYPPCPQPDLTLGL 240

Query: 241 QTHSDIGAITLLIQDDVAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAE 300
           Q+HSDIG ITLLIQDDV GLQVL  Q  WV V+PLSPDAIV+IL DQTEILTNGKCKSAE
Sbjct: 241 QSHSDIGVITLLIQDDVGGLQVLDAQEGWVSVQPLSPDAIVVILGDQTEILTNGKCKSAE 300

Query: 301 HRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEG 360
           HRV+TNSSRARLSISAFHDP KAVKI+PAAELV ESSP+RYREVIYGDYTSSWYS GP G
Sbjct: 301 HRVVTNSSRARLSISAFHDPAKAVKIAPAAELVRESSPVRYREVIYGDYTSSWYSKGPVG 360

Query: 361 RRNLDAVKLHN 371
           RRNLDA+KLHN
Sbjct: 361 RRNLDALKLHN 371

BLAST of HG10002550 vs. TAIR 10
Match: AT4G16330.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 370.5 bits (950), Expect = 1.5e-102
Identity = 192/329 (58.36%), Postives = 237/329 (72.04%), Query Frame = 0

Query: 39  HRPSDTNNSTDLRIPVIDLFGFDPAHRQAVRADIGRESSEWGAFQVINHGIPISLLNQMR 98
           H P D  +     IP +DL   D A     R  IG    +WGAF VINHG+PI LL++MR
Sbjct: 59  HYPGDAAS-----IPTVDLSSSDSA-----REAIGDACRDWGAFHVINHGVPIHLLDRMR 118

Query: 99  TAALSFFRDCPISDKLAYACDK-SFASEGYGTQMLLDKGVDSQSSVLDWRDYFDHHTLPL 158
           +  LSFF+D P+ +KL YACD  S ASEGYG++MLL    D    VLDWRDYFDHHT P 
Sbjct: 119 SLGLSFFQDSPMEEKLRYACDSTSAASEGYGSRMLLGAKDD---VVLDWRDYFDHHTFPP 178

Query: 159 SRRNPSNWPHFPSNYRELMAQYSDCMNLLAQRLLALISESIGLPSSWMEDAITGRGAEEG 218
           SRRNPS+WP  PS+YR+++ +Y D M  LAQ LL LISES+GLP S +E+A+        
Sbjct: 179 SRRNPSHWPIHPSDYRQVVGEYGDEMKKLAQMLLGLISESLGLPCSSIEEAV------GE 238

Query: 219 FYQNITVSYYPPCPQPDLTLGLQTHSDIGAITLLIQDDVAGLQVLSGQGDWVMVEPLSPD 278
            YQNITV+YYPPCPQP+LTLGLQ+HSD GAITLLIQDDV GLQ L     W+ V P+S D
Sbjct: 239 IYQNITVTYYPPCPQPELTLGLQSHSDFGAITLLIQDDVEGLQ-LYKDAQWLTVPPIS-D 298

Query: 279 AIVIILADQTEILTNGKCKSAEHRVITNSSRARLSISAFHDPPKAVKISPAAELVSESSP 338
           AI+I++ADQTEI+TNG+ KSA+HR +TN++RARLS++ FHDP K  +I+P    VS+ SP
Sbjct: 299 AILILIADQTEIITNGRYKSAQHRAVTNANRARLSVATFHDPSKTARIAP----VSQLSP 358

Query: 339 LRYREVIYGDYTSSWYSNGPEGRRNLDAV 367
             Y+EV+YG Y SSWYS GPEG+RNLDA+
Sbjct: 359 PSYKEVVYGQYVSSWYSKGPEGKRNLDAL 362

BLAST of HG10002550 vs. TAIR 10
Match: AT4G16330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 328.9 bits (842), Expect = 5.1e-90
Identity = 167/271 (61.62%), Postives = 206/271 (76.01%), Query Frame = 0

Query: 97  MRTAALSFFRDCPISDKLAYACDK-SFASEGYGTQMLLDKGVDSQSSVLDWRDYFDHHTL 156
           MR+  LSFF+D P+ +KL YACD  S ASEGYG++MLL    D    VLDWRDYFDHHT 
Sbjct: 1   MRSLGLSFFQDSPMEEKLRYACDSTSAASEGYGSRMLLGAKDD---VVLDWRDYFDHHTF 60

Query: 157 PLSRRNPSNWPHFPSNYRELMAQYSDCMNLLAQRLLALISESIGLPSSWMEDAITGRGAE 216
           P SRRNPS+WP  PS+YR+++ +Y D M  LAQ LL LISES+GLP S +E+A+      
Sbjct: 61  PPSRRNPSHWPIHPSDYRQVVGEYGDEMKKLAQMLLGLISESLGLPCSSIEEAV------ 120

Query: 217 EGFYQNITVSYYPPCPQPDLTLGLQTHSDIGAITLLIQDDVAGLQVLSGQGDWVMVEPLS 276
              YQNITV+YYPPCPQP+LTLGLQ+HSD GAITLLIQDDV GLQ L     W+ V P+S
Sbjct: 121 GEIYQNITVTYYPPCPQPELTLGLQSHSDFGAITLLIQDDVEGLQ-LYKDAQWLTVPPIS 180

Query: 277 PDAIVIILADQTEILTNGKCKSAEHRVITNSSRARLSISAFHDPPKAVKISPAAELVSES 336
            DAI+I++ADQTEI+TNG+ KSA+HR +TN++RARLS++ FHDP K  +I+P    VS+ 
Sbjct: 181 -DAILILIADQTEIITNGRYKSAQHRAVTNANRARLSVATFHDPSKTARIAP----VSQL 240

Query: 337 SPLRYREVIYGDYTSSWYSNGPEGRRNLDAV 367
           SP  Y+EV+YG Y SSWYS GPEG+RNLDA+
Sbjct: 241 SPPSYKEVVYGQYVSSWYSKGPEGKRNLDAL 256

BLAST of HG10002550 vs. TAIR 10
Match: AT2G38240.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 235.7 bits (600), Expect = 5.9e-62
Identity = 138/361 (38.23%), Postives = 213/361 (59.00%), Query Frame = 0

Query: 13  EPPIRVQKLVETQPSQVPPQYIQPPHHRP--SDTNNSTDLRIPVIDLFGF--DPAHRQAV 72
           EP + VQ L +T    VP +Y++P H RP  + T +   + IPV+D+      P   + V
Sbjct: 7   EPIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPEGLRLV 66

Query: 73  RADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACDKSFASEGYG 132
           R+       EWG FQ++NHG+  SL+ ++R A   FF + P+ +K  YA +     EGYG
Sbjct: 67  RS----ACEEWGFFQMVNHGVTHSLMERVRGAWREFF-ELPLEEKRKYA-NSPDTYEGYG 126

Query: 133 TQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQYSDCMNLLAQ 192
           +++ + K      + LDW DYF  + LP S RNPS WP  P   REL+ +Y + +  L +
Sbjct: 127 SRLGVVK-----DAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCE 186

Query: 193 RLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGLQTHSDIGAI 252
           RL   +SES+GL  + +  A+ G G + G   ++  ++YP CPQP LTLGL +HSD G I
Sbjct: 187 RLTETLSESLGLKPNKLMQAL-GGGDKVG--ASLRTNFYPKCPQPQLTLGLSSHSDPGGI 246

Query: 253 TLLIQDD-VAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAEHRVITNSS 312
           T+L+ D+ VAGLQV  G G WV ++ + P+A+++ + DQ +IL+NG  KS EH+VI NS 
Sbjct: 247 TILLPDEKVAGLQVRRGDG-WVTIKSV-PNALIVNIGDQLQILSNGIYKSVEHQVIVNSG 306

Query: 313 RARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEGRRNLDAVK 369
             R+S++ F++P   + + P  ELV+ + P  Y+ + + +Y S     GP G+  +D++ 
Sbjct: 307 MERVSLAFFYNPRSDIPVGPIEELVTANRPALYKPIRFDEYRSLIRQKGPCGKNQVDSLL 351

BLAST of HG10002550 vs. TAIR 10
Match: AT3G11180.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 6.5e-61
Identity = 132/359 (36.77%), Postives = 203/359 (56.55%), Query Frame = 0

Query: 13  EPPIRVQKLVETQPSQVPPQYIQPPHHRPSDT-----NNSTDLRIPVIDLFGFDPAHRQA 72
           EP +RVQ L E+  + +P +YI+PP  RP  T         D+ IP+IDL      +   
Sbjct: 51  EPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNEDD 110

Query: 73  VRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACDKSFASEGY 132
            +  I     EWG FQVINHG+   L++  R    SFF + P+  K  Y+ +     EGY
Sbjct: 111 -KKRISEACREWGFFQVINHGVKPELMDAARETWKSFF-NLPVEAKEVYS-NSPRTYEGY 170

Query: 133 GTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQYSDCMNLLA 192
           G+++ ++KG     ++LDW DY+  H LPL+ ++ + WP  PSN RE+  +Y   +  L 
Sbjct: 171 GSRLGVEKG-----AILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLG 230

Query: 193 QRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGLQTHSDIGA 252
            RL+ ++S ++GL +  +++A  G    E     + V+YYP CPQP+L LGL  HSD G 
Sbjct: 231 GRLMTILSSNLGLRAEQLQEAFGG----EDVGACLRVNYYPKCPQPELALGLSPHSDPGG 290

Query: 253 ITLLIQDD-VAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAEHRVITNS 312
           +T+L+ DD V GLQV  G   W+ V PL   A ++ + DQ +IL+N K KS EHRVI NS
Sbjct: 291 MTILLPDDQVVGLQVRHGD-TWITVNPLR-HAFIVNIGDQIQILSNSKYKSVEHRVIVNS 350

Query: 313 SRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDYTSSWYSNGPEGRRNLDA 366
            + R+S++ F++P   + I P  +LV+ + P  Y  + +  Y     + GP G+ ++++
Sbjct: 351 EKERVSLAFFYNPKSDIPIQPMQQLVTSTMPPLYPPMTFDQYRLFIRTQGPRGKSHVES 395

BLAST of HG10002550 vs. TAIR 10
Match: AT3G11180.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 225.7 bits (574), Expect = 6.1e-59
Identity = 129/342 (37.72%), Postives = 194/342 (56.73%), Query Frame = 0

Query: 13  EPPIRVQKLVETQPSQVPPQYIQPPHHRPSDT-----NNSTDLRIPVIDLFGFDPAHRQA 72
           EP +RVQ L E+  + +P +YI+PP  RP  T         D+ IP+IDL      +   
Sbjct: 51  EPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNEDD 110

Query: 73  VRADIGRESSEWGAFQVINHGIPISLLNQMRTAALSFFRDCPISDKLAYACDKSFASEGY 132
            +  I     EWG FQVINHG+   L++  R    SFF + P+  K  Y+ +     EGY
Sbjct: 111 -KKRISEACREWGFFQVINHGVKPELMDAARETWKSFF-NLPVEAKEVYS-NSPRTYEGY 170

Query: 133 GTQMLLDKGVDSQSSVLDWRDYFDHHTLPLSRRNPSNWPHFPSNYRELMAQYSDCMNLLA 192
           G+++ ++KG     ++LDW DY+  H LPL+ ++ + WP  PSN RE+  +Y   +  L 
Sbjct: 171 GSRLGVEKG-----AILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLG 230

Query: 193 QRLLALISESIGLPSSWMEDAITGRGAEEGFYQNITVSYYPPCPQPDLTLGLQTHSDIGA 252
            RL+ ++S ++GL +  +++A  G    E     + V+YYP CPQP+L LGL  HSD G 
Sbjct: 231 GRLMTILSSNLGLRAEQLQEAFGG----EDVGACLRVNYYPKCPQPELALGLSPHSDPGG 290

Query: 253 ITLLIQDD-VAGLQVLSGQGDWVMVEPLSPDAIVIILADQTEILTNGKCKSAEHRVITNS 312
           +T+L+ DD V GLQV  G   W+ V PL   A ++ + DQ +IL+N K KS EHRVI NS
Sbjct: 291 MTILLPDDQVVGLQVRHGD-TWITVNPLR-HAFIVNIGDQIQILSNSKYKSVEHRVIVNS 350

Query: 313 SRARLSISAFHDPPKAVKISPAAELVSESSPLRYREVIYGDY 349
            + R+S++ F++P   + I P  +LV+ + P  Y  + +  Y
Sbjct: 351 EKERVSLAFFYNPKSDIPIQPMQQLVTSTMPPLYPPMTFDQY 378

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895425.11.5e-19792.72probable 2-oxoglutarate-dependent dioxygenase ANS [Benincasa hispida] >XP_038895... [more]
XP_008451879.11.1e-19090.30PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase 5 isoform X1 [Cucumis melo][more]
KAG7015103.13.8e-18887.87putative 2-oxoglutarate-dependent dioxygenase ANS [Cucurbita argyrosperma subsp.... [more]
XP_022931353.13.8e-18887.87probable 2-oxoglutarate-dependent dioxygenase ANS isoform X1 [Cucurbita moschata... [more]
XP_022985093.18.5e-18887.33probable 2-oxoglutarate-dependent dioxygenase ANS [Cucurbita maxima] >XP_0229850... [more]
Match NameE-valueIdentityDescription
O804498.2e-6138.23Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1[more]
Q9SRM39.1e-6036.77Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1[more]
Q9FFF64.7e-5635.47Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1[more]
Q9LY481.4e-5534.99Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1[more]
Q8W2X55.9e-5137.09Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1... [more]
Match NameE-valueIdentityDescription
A0A1S3BTN25.2e-19190.301-aminocyclopropane-1-carboxylate oxidase 5 isoform X1 OS=Cucumis melo OX=3656 G... [more]
A0A6J1EYB11.8e-18887.87probable 2-oxoglutarate-dependent dioxygenase ANS isoform X1 OS=Cucurbita moscha... [more]
A0A6J1JAE54.1e-18887.33probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita maxima OX=3661 GN... [more]
A0A0A0L0367.0e-18888.68Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G... [more]
A0A6J1C4081.6e-17682.48probable 2-oxoglutarate-dependent dioxygenase ANS OS=Momordica charantia OX=3673... [more]
Match NameE-valueIdentityDescription
AT4G16330.21.5e-10258.362-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G16330.15.1e-9061.622-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G38240.15.9e-6238.232-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G11180.16.5e-6136.772-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G11180.26.1e-5937.722-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 52..167
e-value: 1.1E-18
score: 68.1
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 224..318
e-value: 2.5E-20
score: 72.8
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 16..355
e-value: 1.8E-91
score: 308.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..47
NoneNo IPR availablePANTHERPTHR47990:SF1452-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEINcoord: 14..368
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 14..368
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 16..356
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 217..319
score: 11.277682

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10002550.1HG10002550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding