HG10002354 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10002354
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionAcetyltransferase component of pyruvate dehydrogenase complex
LocationChr11: 5911635 .. 5917559 (-)
RNA-Seq ExpressionHG10002354
SyntenyHG10002354
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTACGCATCGCAAATTATCAACCATTCTAAAAAGGTGAGGTTCAATTCGAATATCACATCACTTCCGTAATCTGTGACTTACTGATTTATCTGTTTCCAATTTGATTTTGGAATTACTCTCTGAATCGTTCTGGGTGTAAGCTTCGTGATGTGTATCATTTGCATGTTGCACAAATAGGAGCGCATATATTTTGTTTTGTGGTCTTCCAAATTTGTTTAGTCTCTTTACAGTATTGCACACAAAATCTAGCTGCTATGTCGAATGGGATAATTAAGCTAATACTTATTTATGGACACTAATTTTCTAATGGGCATTTCATTTTAATTGCACTGTTGGTTTTTGTACGGTTCTTTATGGTTCTCTTATTTTCTCTCTTCATTTTCCCCTCATCGTGCTATTGAACCTTACTGATTCTCCTTGTTCCACAAATAAGCTTTGGGCGGTGTTTCTATATTTGGTTTTCGTTTCTTTTGGTGTGGGGTGTGGTGGGGGTTGGGGGCAGTTGGCTAATATTAGTTTCAACCCTTCTTGCTGGGTCTCAGTTTTAGCTGGAGGGAGGTTCCTTTTATCTGTTATTGTGGATTTGAACAGTTGTTCATGCTTATTGACTGCCGCCTAATTTTTGTTGCATTGCCTGATTTATAGTTGTCTTGTAGTTTTCAACTTGGATACACTGATTTATTATCACAGGTTCATAATTGAACTCTCACTTGCTAATTTATGTCAACAAATTATGGCATGTCCATCATAACAAAGCTATGGAAGAGGTTTGTTGTTTTGCATACTGACAGGGCGTTGGGTGCTCATAAGTCATAAACTAAACGTGGCATCATTTGTTTCTCTTCAATGCTTAAATTGTACCTGCCAGTTAGGGGGCGTTTGGACACTCGTTATGTAATGGGCTTGTAAAGTAATATAATCCAAAGTCCATGTTTTGATTGAGCGTTTTGGGCCCGATTTATAATACTAAACTTATTTCGTTCCAACAGTTTTCAATCTAACCCTCTTTTTCATCATTTTCATACTTCACGATCCCATTTCTGTTCCGCCCTCGATTCCACACGTATTCTAATACCTCTTTGATTCCCAGTAATCCTGATTACATTCCCGCTCCCCAAATAGCCCCTTAGGAATTATATTATACTTCTCACGTAGCTGCATTTATCCTTGATTCTTTATATATATTTATTCCCATGATTCACATTAATCTACTTGAACCTGTTTCTTCTAAAAAGGCATTCGCAATTTTCCGTGGTTTTAATCAGAACATTTATCCCATTCATGTTATTCTTGCAGTTAAGAAGTGCTCCAAAGTTACTTCGGCAAGCTAGCGTGGTTCGCTGGTTTTCAGAGGATGTGGAGTCCTCTATTGTTAAGGACAATGGTAAGCAGTTGTAATCAAGAACTTTGAACCAAGAAAACCTGCTTGGTCAAGTTACTTTTTGGTATTTATTTGCTGTCCTCTAATGTTCTTGATTGTACATCTGAAGTTTAAGAACTCTGCTTGGTAACTTTGGTATTTATTTGATGTAATTAATCAGAAATAATAAATTTTTGTTATGTTGGTCAACTTAACGAAATATATGATTGATTATTTTGCATATTATATAAACTGTTATAACGTGAGGGCTTCTGCCAGCTGACTCTTGAAGTTACCCTAACAAAGGTGGTTGTTTCACAGAAATCTGGAAGATCCAGGGTCGTGGTTCCCAGGCTTCGGAAAAAGGGGGAATCAAGAACCTTGCCAATTTTAACAAAGTGAGTTATAACCACGAGTTTTTCTGTTCATTAAATCATTAATTAATACTTTCTAGCAGATTGAAATGTTGCTTGTCATAATTTCTTCCATCTGCTGTAGCCTGTCACTCTCTACCATGTGCGTAACAACTTCTGATGTAACATGTTTTGTGTTGCATTGTACTCTTCCCTTTGTAGAGATCGCACTTTCTACAATGTCAAAGAGGTGTTTCAGTGATGACGACAAGTATTGGGAATCCTTTTTCTGGGTCAGTATCTAACCACACACACTCGCTTATGCACACACTCACTTATAAAACAAGACATTATCTATCAGCATGTGCAATTTATTTGTGAGTTAGGATGCTAGGGCTCTTATTCTGATCATGATCAAAACTCGAAAGTGTCTAATATCTGTCTTTTCCTCCAACAGTTCACGAATTAACCCAATGAGAGGTTTCTCCTCTGATTCAGGTACATTGTGGTTTTATTTTCCTTTTCCATTCTGCAGACTTACATGAATTTGTTTCATTTTTTCTAATTGATGTTCTGTATATACAGCAAGATTTGGATTTGGATTTTTTTCTTCTTCCATTGTGTGGTTTTGTTTACTTCTAAGTTCTAACTTGATTAAGCATGAAATGCTTGTATACTTCCATTTGTCATTACAACAACTGTAACTGCGATGACCTTACTTGAATAGGATCTAACTATGTACTTGTTAATGATATAACTGCACATCGTGCTGAATTTTGCTAAGATTCTTTTCTCAAATCTTTCTATTTTGATGGAAAATGAGTTGTATGTGCTTTTGAAGAAATATCCACTCTAGCTCCCACAATGATACAAGTTTAAAATCTATGACTAACAATGGGACATTTTCATATTACCTTCTGGAAAACTATCTTCAAATGTACATTTTACATAGAATCATTCAACATGCTTCAGGTCTTCCCCCACATCAAGAGGTTGGAATGCCTTCGCTGTCCCCTACAATGACAGAGGTAGCTTATGGTCACGAACCTGGCAGAATAATATATTGGCAAATAAATGCTAAGTTGTTCTCTATGTTATGCAGGGCAATATTGCAAGGTGGTTGAAAAAAGAGGGTGATAAAATTTCTCCTGGGGAAGTGCTTTGTGAAGTTGAAACGGTATATTATTCTAATATCTTTTTGCAGTTGGAAGTTGAAAGTAGTTTTTTACTGCCTCTTTATGGTAGGATTATAGAACTATAATTTCTGATTTTGCTTCTGCATGCTTGAAGGATAAAGCAACAGTAGAAATGGAATGCATGGAGGAAGGTTATCTTGCCAAGATAATTTGTGGGGATGGAGCGAAGGAAATTAAAGTTGGTGAGGTATTGATAATGCTTTCTATCATTTTTTCCATCCCTCTTATCTTTGTGAAAACTTGAAAGGGAAAACGAGTGATTGTCGTATTGCACCATTATCATAGATGAGGTGATGGCGATGACTGTCATTGAAAGCTCTTTCTCGGGATAGAGGCATATGATATTTTTCCCGCATTTGATGCGGTTGACATAAGTATTGTTTTTGTTTTTTATTTTGTTTTTTTTAATAGGTAATTGCCATTACTGTTGAAGATGAAGAGGATATAGCAAAGTTTAAAGATTATAAACCCACTTCATCAAATACTGGTGCTGCAGCTGCCACGGAGTCATCTGCCCCTACCCCACCAAAGAAAGAAGTAGTTGAGGAACCTGTTCGCTCACCTGAGCCAAAGACTGTTAAGCAGAGTCCTCCTTCTCCAGCTGGAGAGCGTATTTTTGCCAGTCCTCTGGCAAGAAAATTAGCAGAAGAAAACAATGTTCGTTGCATAATCTTTTTCAATTAATTTTTGGAACCTTACGTTTGTGATTTCGTTCTAGTTATATTTTGAATTTTATGAGCATAGGTGCCCATTTCAAGTATCAAAGGAACAGGTCCTGATGGGAGCATTGTGAAGGCGGATATAGAAGATTACTTGGGTAACTCATTTTCTTTATGCTTGGCAGTGGCACCGGCAACTTTATTTATTTATTTATTTTTGTGCTATTGTCTTCTATCTGTTAATATTTGTTTATATTCGACATCTCTGTTGTCTTCATACCCATTAATATGTTTCTTATTGTTAAAAACGTGGCCCATTAACATCTTTTACCTTATGTCTCTAGCTTCACGTGGGAAGGAATCAACAGCTCCCAAAGCCAAGGATGCAGCAGGTGCACCATTGGATTACAGTGATATCCCACATACTCAAATAAGAAAGGTACCACCCGCCCTCCCCATCTCTCTCACCATAAACTCCATGATAGTAAAATTTCTTGTATTTTCTGGCAAGTTTTTAGTGTAATGTGGCGATATTTCACTCCCTGCAGTGCCTTCGTCTATATTCTCAATCTCTTGGCTACCTTATTCCTTAACAATACATACAGGCTTGCATATGTTCTATTAACACAAAAGTACTGCAATTGTTTGTTTTCTTGTATCAGACTCTACACCTTTCAACTGTTAGGATGTTCTAAAAAATAGATTTCTTGTAGTTGTTATATGTTTCATTAGCGGCTTCTGCATTTTCAAATTTGGATCTTATTTATTGGATTACTAGACAAACCATCCTAAATATTCCAACTTTGGACATGTTTTCCGAGAGCTAATATTCCACATTCTTACAGGTTACAGCATCACGATTACTATTCTCAAAGCAAACTATTCCTCATTATTATTTAACTGTAGATACATGCGTTGACAAGCTAATGGAGTAAGTCATTCCTCCACACATTTTGCAGTTAAGTATTAACCAAAGTTATAAATTTAATCAGCAGTTGTTCTTTTTGTAGTTTGAGAAACCAGCTAAATGCACTACAAGAAGCATCTGGTGCTAAGAGGATATCTGTCAATGACCTTGTTATTAAGGTATTTTAAAGAAATTATATAATTGAGTATGTAGTTAAGTTGTTTTTGCTGCATAGGTATTGAAGCAATTATCATCAGTTTGGACTTATTAATTTTTCTTATAATTTGACTAGGACTTTTGCCCTTTTTTCTCCTCATATGCTTTTTTATTTTTTATTTTTTTATTTTTATTTTTATATTAAAACTTAAGCCTCGGGGAGAAACGTACTTCATCTTGCCAACAATTATCTACATCCATTCTATATCAAACCCATTCTATATGTGTGTGTGTGAAAAAAAATCCTTTTAATCATAGAATATTGCTGCAGGCCGCTGCCTTGGCTCTTAGGAAAGTCCCTCAATGCAACAGTTCATGGACGGACAATTATATTAGACAGTAAGTGATAAGACTTGTTAATTTTAATTTACTTATTTTCATTCTCACATTGTAATGTGAATTTTTTTTCTCTGTCCACATCCAGGTATCACAATGTGAATATTAATGTTGCAGTGCAGACCGATAACGGGCTCTTTGTACCAGTCATCAGGGTCAGTAGTTTCTGCACATGCTAGTAAACTAGGTGGCTTTTATTACCATGTTCGAGAGTGTGAATTTGTATAATCTACAGGATGCAGACAAAAAAGGTTTATCTACTATTTCAGACGAGGTCAAGAAACTAGCTCAGAAAGCAAGAGATAACAGCTTAAAACCAGAAGATTATGAGGTGGGCAGTGTTCAATATTGGTTTGATGCTTTAGGAGGAAGATTTACGCTTGGTTTTAGGATTTTGATTAGCAGTTAAATCCTTTTGTAGGGAGGCACTTTTACTGTGTCTAACTTGGGAGGTCCATTTGGAATCAAACAATTTTGTGCCATTATCAACCCTCCACAGTCGGGCATTCTTGCTGTTGGATCTGGTATGCAGACCCATTCATCATTCTCGATATCCAAGTATCTTTTGTTTCTTTTCTTTCATTGCTGTTCTTTAACATTATTAATTGCCATTCACATTTAATCTTGAGCAGCTGAGAAGAGGGTCATACCAGGATCGGGCACCGAAGAATTCAAGTTTGCTTCCTTCATGTCTGTTACACTGAGCTGTGATCACCGCGTTATTGATGGTAGGATTCTGAACCTGAAGTCCTTTTAGATTCTGTAAAACTTGAAAGAGAAATGTAAGGTGTCTAAGATCATGTTGTTCTTTGTTTGTTCAGGGGCAATTGGTGCGGATTGGCTCAAAGCATTCAAGGGCTACATCGAGAATCCTGAATCAATGTTGCTATAG

mRNA sequence

ATGGCTTACGCATCGCAAATTATCAACCATTCTAAAAAGTTAAGAAGTGCTCCAAAGTTACTTCGGCAAGCTAGCGTGGTTCGCTGGTTTTCAGAGGATGTGGAGTCCTCTATTGTTAAGGACAATGAAATCTGGAAGATCCAGGGTCGTGGTTCCCAGGCTTCGGAAAAAGGGGGAATCAAGAACCTTGCCAATTTTAACAAAGATGCTAGGGCTCTTATTCTGATCATGATCAAAACTCGAAAGTGTCTAATATCTGTCTTTTCCTCCAACAGTTCACGAATTAACCCAATGAGAGGTTTCTCCTCTGATTCAGGTCTTCCCCCACATCAAGAGGTTGGAATGCCTTCGCTGTCCCCTACAATGACAGAGGGCAATATTGCAAGGTGGTTGAAAAAAGAGGGTGATAAAATTTCTCCTGGGGAAGTGCTTTGTGAAGTTGAAACGGATAAAGCAACAGTAGAAATGGAATGCATGGAGGAAGGTTATCTTGCCAAGATAATTTGTGGGGATGGAGCGAAGGAAATTAAAGTTGGTGAGGTAATTGCCATTACTGTTGAAGATGAAGAGGATATAGCAAAGTTTAAAGATTATAAACCCACTTCATCAAATACTGGTGCTGCAGCTGCCACGGAGTCATCTGCCCCTACCCCACCAAAGAAAGAAGTAGTTGAGGAACCTGTTCGCTCACCTGAGCCAAAGACTGTTAAGCAGAGTCCTCCTTCTCCAGCTGGAGAGCGTATTTTTGCCAGTCCTCTGGCAAGAAAATTAGCAGAAGAAAACAATGTGCCCATTTCAAGTATCAAAGGAACAGGTCCTGATGGGAGCATTGTGAAGGCGGATATAGAAGATTACTTGGCTTCACGTGGGAAGGAATCAACAGCTCCCAAAGCCAAGGATGCAGCAGGTGCACCATTGGATTACAGTGATATCCCACATACTCAAATAAGAAAGGTTACAGCATCACGATTACTATTCTCAAAGCAAACTATTCCTCATTATTATTTAACTGTAGATACATGCGTTGACAAGCTAATGGATTTGAGAAACCAGCTAAATGCACTACAAGAAGCATCTGGTGCTAAGAGGATATCTGTCAATGACCTTGTTATTAAGGCCGCTGCCTTGGCTCTTAGGAAAGTCCCTCAATGCAACAGTTCATGGACGGACAATTATATTAGACAGTATCACAATGTGAATATTAATGTTGCAGTGCAGACCGATAACGGGCTCTTTGTACCAGTCATCAGGGATGCAGACAAAAAAGGTTTATCTACTATTTCAGACGAGGTCAAGAAACTAGCTCAGAAAGCAAGAGATAACAGCTTAAAACCAGAAGATTATGAGGGAGGCACTTTTACTGTGTCTAACTTGGGAGGTCCATTTGGAATCAAACAATTTTGTGCCATTATCAACCCTCCACAGTCGGGCATTCTTGCTGTTGGATCTGCTGAGAAGAGGGTCATACCAGGATCGGGCACCGAAGAATTCAAGTTTGCTTCCTTCATGTCTGTTACACTGAGCTGTGATCACCGCGTTATTGATGGGGCAATTGGTGCGGATTGGCTCAAAGCATTCAAGGGCTACATCGAGAATCCTGAATCAATGTTGCTATAG

Coding sequence (CDS)

ATGGCTTACGCATCGCAAATTATCAACCATTCTAAAAAGTTAAGAAGTGCTCCAAAGTTACTTCGGCAAGCTAGCGTGGTTCGCTGGTTTTCAGAGGATGTGGAGTCCTCTATTGTTAAGGACAATGAAATCTGGAAGATCCAGGGTCGTGGTTCCCAGGCTTCGGAAAAAGGGGGAATCAAGAACCTTGCCAATTTTAACAAAGATGCTAGGGCTCTTATTCTGATCATGATCAAAACTCGAAAGTGTCTAATATCTGTCTTTTCCTCCAACAGTTCACGAATTAACCCAATGAGAGGTTTCTCCTCTGATTCAGGTCTTCCCCCACATCAAGAGGTTGGAATGCCTTCGCTGTCCCCTACAATGACAGAGGGCAATATTGCAAGGTGGTTGAAAAAAGAGGGTGATAAAATTTCTCCTGGGGAAGTGCTTTGTGAAGTTGAAACGGATAAAGCAACAGTAGAAATGGAATGCATGGAGGAAGGTTATCTTGCCAAGATAATTTGTGGGGATGGAGCGAAGGAAATTAAAGTTGGTGAGGTAATTGCCATTACTGTTGAAGATGAAGAGGATATAGCAAAGTTTAAAGATTATAAACCCACTTCATCAAATACTGGTGCTGCAGCTGCCACGGAGTCATCTGCCCCTACCCCACCAAAGAAAGAAGTAGTTGAGGAACCTGTTCGCTCACCTGAGCCAAAGACTGTTAAGCAGAGTCCTCCTTCTCCAGCTGGAGAGCGTATTTTTGCCAGTCCTCTGGCAAGAAAATTAGCAGAAGAAAACAATGTGCCCATTTCAAGTATCAAAGGAACAGGTCCTGATGGGAGCATTGTGAAGGCGGATATAGAAGATTACTTGGCTTCACGTGGGAAGGAATCAACAGCTCCCAAAGCCAAGGATGCAGCAGGTGCACCATTGGATTACAGTGATATCCCACATACTCAAATAAGAAAGGTTACAGCATCACGATTACTATTCTCAAAGCAAACTATTCCTCATTATTATTTAACTGTAGATACATGCGTTGACAAGCTAATGGATTTGAGAAACCAGCTAAATGCACTACAAGAAGCATCTGGTGCTAAGAGGATATCTGTCAATGACCTTGTTATTAAGGCCGCTGCCTTGGCTCTTAGGAAAGTCCCTCAATGCAACAGTTCATGGACGGACAATTATATTAGACAGTATCACAATGTGAATATTAATGTTGCAGTGCAGACCGATAACGGGCTCTTTGTACCAGTCATCAGGGATGCAGACAAAAAAGGTTTATCTACTATTTCAGACGAGGTCAAGAAACTAGCTCAGAAAGCAAGAGATAACAGCTTAAAACCAGAAGATTATGAGGGAGGCACTTTTACTGTGTCTAACTTGGGAGGTCCATTTGGAATCAAACAATTTTGTGCCATTATCAACCCTCCACAGTCGGGCATTCTTGCTGTTGGATCTGCTGAGAAGAGGGTCATACCAGGATCGGGCACCGAAGAATTCAAGTTTGCTTCCTTCATGTCTGTTACACTGAGCTGTGATCACCGCGTTATTGATGGGGCAATTGGTGCGGATTGGCTCAAAGCATTCAAGGGCTACATCGAGAATCCTGAATCAATGTTGCTATAG

Protein sequence

MAYASQIINHSKKLRSAPKLLRQASVVRWFSEDVESSIVKDNEIWKIQGRGSQASEKGGIKNLANFNKDARALILIMIKTRKCLISVFSSNSSRINPMRGFSSDSGLPPHQEVGMPSLSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGEVIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVVEEPVRSPEPKTVKQSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKDAAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASGAKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILAVGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESMLL
Homology
BLAST of HG10002354 vs. NCBI nr
Match: XP_038904277.1 (dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial [Benincasa hispida] >XP_038904281.1 dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial [Benincasa hispida] >XP_038904287.1 dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial [Benincasa hispida])

HSP 1 Score: 968.4 bits (2502), Expect = 2.6e-278
Identity = 500/541 (92.42%), Postives = 508/541 (93.90%), Query Frame = 0

Query: 1   MAYASQIINHSKKLRSAPKLLRQASVVRWFSEDVESSIVKDNEIWKIQGRGSQASEKGGI 60
           MAYASQIINHSKKLRSAPKLLRQASVVRWFSEDV+SSIVKDNEIWKIQGRG QA EKGGI
Sbjct: 1   MAYASQIINHSKKLRSAPKLLRQASVVRWFSEDVQSSIVKDNEIWKIQGRGYQAWEKGGI 60

Query: 61  KNLANFNKDARALILIMIKTRKCLISVFSSN---SSRINPMRGFSSDSGLPPHQEVGMPS 120
           KN ANFN  +     +  +    +++    N    SRIN MRGFSSDSGLPPHQEVGMPS
Sbjct: 61  KNYANFNNRSH---FLQCQRGVSMMTTSIGNPFSGSRINAMRGFSSDSGLPPHQEVGMPS 120

Query: 121 LSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIK 180
           LSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIK
Sbjct: 121 LSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIK 180

Query: 181 VGEVIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVVEEPVRSPEPKTVK 240
           VGEVIAITVEDEEDIAKFKDYKPTSSNTGA AA E SAP+PPKKEVVEEPVRS EPKTVK
Sbjct: 181 VGEVIAITVEDEEDIAKFKDYKPTSSNTGAPAAAEKSAPSPPKKEVVEEPVRSSEPKTVK 240

Query: 241 QSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPK 300
           QSPP P GERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPK
Sbjct: 241 QSPPPPVGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPK 300

Query: 301 AKDAAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQE 360
           AKDAAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQE
Sbjct: 301 AKDAAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQE 360

Query: 361 ASGAKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIR 420
           ASG KRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIR
Sbjct: 361 ASGGKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIR 420

Query: 421 DADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSG 480
           DADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSG
Sbjct: 421 DADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSG 480

Query: 481 ILAVGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESML 539
           ILAVGSAEKRVIPGSG EEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESML
Sbjct: 481 ILAVGSAEKRVIPGSGAEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESML 538

BLAST of HG10002354 vs. NCBI nr
Match: XP_008447623.1 (PREDICTED: dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex, mitochondrial [Cucumis melo] >ADN33731.1 dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase [Cucumis melo subsp. melo])

HSP 1 Score: 953.0 bits (2462), Expect = 1.1e-273
Identity = 490/538 (91.08%), Postives = 505/538 (93.87%), Query Frame = 0

Query: 1   MAYASQIINHSKKLRSAPKLLRQASVVRWFSEDVESSIVKDNEIWKIQGRGSQASEKGGI 60
           MAYASQIINHSKKLRS PKL RQAS+VRWFSED +SSI  DNEIWKIQG G +AS KGGI
Sbjct: 1   MAYASQIINHSKKLRSTPKLFRQASMVRWFSEDAQSSI--DNEIWKIQGSGYKASGKGGI 60

Query: 61  KNLANFNKDARALILIMIKTRKCLISVFSSNSSRINPMRGFSSDSGLPPHQEVGMPSLSP 120
           KNLANFN  ++ LI     +          + S+INP RGFSSDSGLPPHQEVGMPSLSP
Sbjct: 61  KNLANFNNRSQFLICQRGVSMMATSIGNPFSGSQINPARGFSSDSGLPPHQEVGMPSLSP 120

Query: 121 TMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGE 180
           TMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGE
Sbjct: 121 TMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGE 180

Query: 181 VIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVVEEPVRSPEPKTVKQSP 240
           VIAITVEDEEDIAKFKDYKPTSSNTGAA+A ES AP+PPKKEVVEEPVRSP+P TVKQSP
Sbjct: 181 VIAITVEDEEDIAKFKDYKPTSSNTGAASAPESPAPSPPKKEVVEEPVRSPQPSTVKQSP 240

Query: 241 PSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKD 300
           PSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKD
Sbjct: 241 PSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKD 300

Query: 301 AAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASG 360
           AAGAPLDYSD+PHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASG
Sbjct: 301 AAGAPLDYSDLPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASG 360

Query: 361 AKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDAD 420
            KRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDAD
Sbjct: 361 GKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDAD 420

Query: 421 KKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILA 480
           KKGLSTIS+EVKKLAQKARDN+LKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILA
Sbjct: 421 KKGLSTISNEVKKLAQKARDNTLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILA 480

Query: 481 VGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESMLL 539
           VGSAEKRVIPGSG +EFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESMLL
Sbjct: 481 VGSAEKRVIPGSGAQEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESMLL 536

BLAST of HG10002354 vs. NCBI nr
Match: XP_004146813.1 (dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial isoform X2 [Cucumis sativus])

HSP 1 Score: 952.6 bits (2461), Expect = 1.5e-273
Identity = 488/541 (90.20%), Postives = 506/541 (93.53%), Query Frame = 0

Query: 1   MAYASQIINHSKKLRSAPKLLRQASVVRWFSEDVESSIVKDNEIWKIQGRGSQASEKGGI 60
           MAYASQIINHSKKLRS PKLLRQASVVRWFSED +SSI KDNEIWKIQG G +AS KGGI
Sbjct: 1   MAYASQIINHSKKLRSTPKLLRQASVVRWFSEDAQSSIAKDNEIWKIQGCGYKASGKGGI 60

Query: 61  KNLANFNKDARALILIMIKTRKCLISVFSSN---SSRINPMRGFSSDSGLPPHQEVGMPS 120
           KNLANFN  ++    +  +    +++    N    S+INP RGFSSDSGLPPHQEVGMPS
Sbjct: 61  KNLANFNNRSQ---FLQCQRGVSMMTTSIGNPFSGSQINPARGFSSDSGLPPHQEVGMPS 120

Query: 121 LSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIK 180
           LSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIK
Sbjct: 121 LSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIK 180

Query: 181 VGEVIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVVEEPVRSPEPKTVK 240
           VGEVIAITVEDEEDIAKFKDYKP SSNTGAA+A ES A +PPKKEVVEEPVRSPEPKTVK
Sbjct: 181 VGEVIAITVEDEEDIAKFKDYKPASSNTGAASAAESPASSPPKKEVVEEPVRSPEPKTVK 240

Query: 241 QSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPK 300
           QSPP PAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPK
Sbjct: 241 QSPPPPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPK 300

Query: 301 AKDAAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQE 360
           AKDAAGAPLDYSD+PHTQIRK+TASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQE
Sbjct: 301 AKDAAGAPLDYSDLPHTQIRKITASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQE 360

Query: 361 ASGAKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIR 420
           ASG KRISVNDLVIKAAALAL+KVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIR
Sbjct: 361 ASGGKRISVNDLVIKAAALALKKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIR 420

Query: 421 DADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSG 480
           DADKKGLS ISDEVKKLAQKARDN+LKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSG
Sbjct: 421 DADKKGLSAISDEVKKLAQKARDNTLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSG 480

Query: 481 ILAVGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESML 539
           ILAVGSAEKRVIPGSG +EFKFASFMSVTLSCDHRVIDGAIGADWLKAFKG+IENPESML
Sbjct: 481 ILAVGSAEKRVIPGSGAQEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGFIENPESML 538

BLAST of HG10002354 vs. NCBI nr
Match: KGN59926.2 (hypothetical protein Csa_002187 [Cucumis sativus])

HSP 1 Score: 935.6 bits (2417), Expect = 1.9e-268
Identity = 488/574 (85.02%), Postives = 506/574 (88.15%), Query Frame = 0

Query: 1   MAYASQIINHSKK---------------------------------LRSAPKLLRQASVV 60
           MAYASQIINHSKK                                 LRS PKLLRQASVV
Sbjct: 1   MAYASQIINHSKKLQPFMLDLSFSWREVPFTCFCGLELLLMLIGFRLRSTPKLLRQASVV 60

Query: 61  RWFSEDVESSIVKDNEIWKIQGRGSQASEKGGIKNLANFNKDARALILIMIKTRKCLISV 120
           RWFSED +SSI KDNEIWKIQG G +AS KGGIKNLANFN  ++    +  +    +++ 
Sbjct: 61  RWFSEDAQSSIAKDNEIWKIQGCGYKASGKGGIKNLANFNNRSQ---FLQCQRGVSMMTT 120

Query: 121 FSSN---SSRINPMRGFSSDSGLPPHQEVGMPSLSPTMTEGNIARWLKKEGDKISPGEVL 180
              N    S+INP RGFSSDSGLPPHQEVGMPSLSPTMTEGNIARWLKKEGDKISPGEVL
Sbjct: 121 SIGNPFSGSQINPARGFSSDSGLPPHQEVGMPSLSPTMTEGNIARWLKKEGDKISPGEVL 180

Query: 181 CEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGEVIAITVEDEEDIAKFKDYKPTSSN 240
           CEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGEVIAITVEDEEDIAKFKDYKP SSN
Sbjct: 181 CEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGEVIAITVEDEEDIAKFKDYKPASSN 240

Query: 241 TGAAAATESSAPTPPKKEVVEEPVRSPEPKTVKQSPPSPAGERIFASPLARKLAEENNVP 300
           TGAA+A ES A +PPKKEVVEEPVRSPEPKTVKQSPP PAGERIFASPLARKLAEENNVP
Sbjct: 241 TGAASAAESPASSPPKKEVVEEPVRSPEPKTVKQSPPPPAGERIFASPLARKLAEENNVP 300

Query: 301 ISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKDAAGAPLDYSDIPHTQIRKVTASRL 360
           ISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKDAAGAPLDYSD+PHTQIRK+TASRL
Sbjct: 301 ISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKDAAGAPLDYSDLPHTQIRKITASRL 360

Query: 361 LFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASGAKRISVNDLVIKAAALALRKVPQC 420
           LFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASG KRISVNDLVIKAAALAL+KVPQC
Sbjct: 361 LFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASGGKRISVNDLVIKAAALALKKVPQC 420

Query: 421 NSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDADKKGLSTISDEVKKLAQKARDNSLK 480
           NSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDADKKGLS ISDEVKKLAQKARDN+LK
Sbjct: 421 NSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDADKKGLSAISDEVKKLAQKARDNTLK 480

Query: 481 PEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILAVGSAEKRVIPGSGTEEFKFASFMS 539
           PEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILAVGSAEKRVIPGSG +EFKFASFMS
Sbjct: 481 PEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILAVGSAEKRVIPGSGAQEFKFASFMS 540

BLAST of HG10002354 vs. NCBI nr
Match: XP_031739329.1 (dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial isoform X1 [Cucumis sativus])

HSP 1 Score: 928.7 bits (2399), Expect = 2.3e-266
Identity = 475/529 (89.79%), Postives = 494/529 (93.38%), Query Frame = 0

Query: 13  KLRSAPKLLRQASVVRWFSEDVESSIVKDNEIWKIQGRGSQASEKGGIKNLANFNKDARA 72
           +LRS PKLLRQASVVRWFSED +SSI KDNEIWKIQG G +AS KGGIKNLANFN  ++ 
Sbjct: 29  RLRSTPKLLRQASVVRWFSEDAQSSIAKDNEIWKIQGCGYKASGKGGIKNLANFNNRSQ- 88

Query: 73  LILIMIKTRKCLISVFSSN---SSRINPMRGFSSDSGLPPHQEVGMPSLSPTMTEGNIAR 132
              +  +    +++    N    S+INP RGFSSDSGLPPHQEVGMPSLSPTMTEGNIAR
Sbjct: 89  --FLQCQRGVSMMTTSIGNPFSGSQINPARGFSSDSGLPPHQEVGMPSLSPTMTEGNIAR 148

Query: 133 WLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGEVIAITVEDE 192
           WLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGEVIAITVEDE
Sbjct: 149 WLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGEVIAITVEDE 208

Query: 193 EDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVVEEPVRSPEPKTVKQSPPSPAGERIF 252
           EDIAKFKDYKP SSNTGAA+A ES A +PPKKEVVEEPVRSPEPKTVKQSPP PAGERIF
Sbjct: 209 EDIAKFKDYKPASSNTGAASAAESPASSPPKKEVVEEPVRSPEPKTVKQSPPPPAGERIF 268

Query: 253 ASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKDAAGAPLDYS 312
           ASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKDAAGAPLDYS
Sbjct: 269 ASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKDAAGAPLDYS 328

Query: 313 DIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASGAKRISVNDL 372
           D+PHTQIRK+TASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASG KRISVNDL
Sbjct: 329 DLPHTQIRKITASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASGGKRISVNDL 388

Query: 373 VIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDADKKGLSTISD 432
           VIKAAALAL+KVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDADKKGLS ISD
Sbjct: 389 VIKAAALALKKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDADKKGLSAISD 448

Query: 433 EVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILAVGSAEKRVI 492
           EVKKLAQKARDN+LKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILAVGSAEKRVI
Sbjct: 449 EVKKLAQKARDNTLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILAVGSAEKRVI 508

Query: 493 PGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESMLL 539
           PGSG +EFKFASFMSVTLSCDHRVIDGAIGADWLKAFKG+IENPESMLL
Sbjct: 509 PGSGAQEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGFIENPESMLL 554

BLAST of HG10002354 vs. ExPASy Swiss-Prot
Match: Q8RWN9 (Dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g13930 PE=1 SV=2)

HSP 1 Score: 708.8 bits (1828), Expect = 4.8e-203
Identity = 378/544 (69.49%), Postives = 439/544 (80.70%), Query Frame = 0

Query: 4   ASQIINHSKKLRSAPKLLRQ--ASVVRWFSEDVESSIVKDNEIWKIQGRGSQASE----- 63
           AS+IINHSKKL+    LLR+  A  VR FS     S+V   +I+K +   S         
Sbjct: 2   ASRIINHSKKLKHVSALLRRDHAVAVRCFSNSTHPSLVGREDIFKARLNYSSVERISKCG 61

Query: 64  KGGIKNLANFNKDARALILIMIKTRKCLISVFSSNSSRINPMRGFSSDSGLPPHQEVGMP 123
            G +  L+  +  +  L   M   +  L   F   SS++  +RGFSS S LPPHQE+GMP
Sbjct: 62  TGNVTMLSGISTTSTKLSSPMAGPK--LFKEFI--SSQMRSVRGFSSSSDLPPHQEIGMP 121

Query: 124 SLSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEI 183
           SLSPTMTEGNIARWLKKEGDK++PGEVLCEVETDKATVEMECMEEG+LAKI+  +GAKEI
Sbjct: 122 SLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGFLAKIVKEEGAKEI 181

Query: 184 KVGEVIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVVEEPVRSPEPKTV 243
           +VGEVIAITVEDE+DI KFKDY P SS+TG AA     AP+ PK+E VE+P  +PE K  
Sbjct: 182 QVGEVIAITVEDEDDIQKFKDYTP-SSDTGPAAPEAKPAPSLPKEEKVEKPASAPEAKIS 241

Query: 244 KQSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAP 303
           K S  +P+ +RIFASPLARKLAE+NNVP+SSIKGTGP+G IVKAD+ED+LAS  KE+TA 
Sbjct: 242 KPS-SAPSEDRIFASPLARKLAEDNNVPLSSIKGTGPEGRIVKADVEDFLASGSKETTAK 301

Query: 304 KAK--DAAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNA 363
            +K  D+    LDY DIPHTQIRKVTASRL FSKQTIPHYYLTVDTCVDK+M LR+QLN+
Sbjct: 302 PSKQVDSKVPALDYVDIPHTQIRKVTASRLAFSKQTIPHYYLTVDTCVDKMMGLRSQLNS 361

Query: 364 LQEASGAKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVP 423
            QEASG KRISVNDLVIKAAALALRKVPQCNSSWTD YIRQ+ NVNINVAVQT+NGL+VP
Sbjct: 362 FQEASGGKRISVNDLVIKAAALALRKVPQCNSSWTDEYIRQFKNVNINVAVQTENGLYVP 421

Query: 424 VIRDADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPP 483
           V++DADKKGLSTI +EV+ LAQKA++NSLKPEDYEGGTFTVSNLGGPFGIKQFCA+INPP
Sbjct: 422 VVKDADKKGLSTIGEEVRFLAQKAKENSLKPEDYEGGTFTVSNLGGPFGIKQFCAVINPP 481

Query: 484 QSGILAVGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPE 539
           Q+ ILA+GSAEKRV+PG+G +++  AS+MSVTLSCDHRVIDGAIGA+WLKAFKGYIE PE
Sbjct: 482 QAAILAIGSAEKRVVPGTGPDQYNVASYMSVTLSCDHRVIDGAIGAEWLKAFKGYIETPE 539

BLAST of HG10002354 vs. ExPASy Swiss-Prot
Match: Q5M729 (Dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g54220 PE=1 SV=1)

HSP 1 Score: 706.1 bits (1821), Expect = 3.1e-202
Identity = 369/548 (67.34%), Postives = 438/548 (79.93%), Query Frame = 0

Query: 1   MAYASQIINHSKKLRSAPKLLRQ--ASVVRWFSEDVESSIVKDNEIWKIQGRGSQASEKG 60
           MAYAS+IINHSKKL+    LLR+  A+ +R++S    + + +++      G         
Sbjct: 1   MAYASRIINHSKKLKDVSTLLRRENAATIRYYSNTNRAPLNREDTFNSRLGYPP------ 60

Query: 61  GIKNLANFNKDARALILIMIKTRKCLIS-----VFSSN-SSRINPMRGFSSDSGLPPHQE 120
            ++ ++  +     + +I   TR  L S     +F    S  +   RGFSS S LPPHQE
Sbjct: 61  -LERISICSTSTLPVSIIFSTTRSNLSSAMGRPIFGKEFSCLMQSARGFSSGSDLPPHQE 120

Query: 121 VGMPSLSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDG 180
           +GMPSLSPTMTEGNIARWLKKEGDK++PGEVLCEVETDKATVEMECMEEGYLAKI+  +G
Sbjct: 121 IGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYLAKIVKAEG 180

Query: 181 AKEIKVGEVIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVVEEPVRSPE 240
           +KEI+VGEVIAITVEDEEDI KFKDY P SS   AA       P PPK+E V++P   PE
Sbjct: 181 SKEIQVGEVIAITVEDEEDIGKFKDYTP-SSTADAAPTKAEPTPAPPKEEKVKQPSSPPE 240

Query: 241 PKTVKQSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKE 300
           PK  K S P P G+R+FASPLARKLAE+NNVP+S I+GTGP+G IVKADI++YLAS GK 
Sbjct: 241 PKASKPSTP-PTGDRVFASPLARKLAEDNNVPLSDIEGTGPEGRIVKADIDEYLASSGKG 300

Query: 301 STA--PKAKDAAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRN 360
           +TA   K+ D+    LDY DIPH+QIRKVTASRL FSKQTIPHYYLTVDTCVDKLM LR+
Sbjct: 301 ATAKPSKSTDSKAPALDYVDIPHSQIRKVTASRLAFSKQTIPHYYLTVDTCVDKLMALRS 360

Query: 361 QLNALQEASGAKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNG 420
           QLN+ +EASG KRISVNDLV+KAAALALRKVPQCNSSWTD+YIRQ+ NVNINVAVQT+NG
Sbjct: 361 QLNSFKEASGGKRISVNDLVVKAAALALRKVPQCNSSWTDDYIRQFKNVNINVAVQTENG 420

Query: 421 LFVPVIRDADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAI 480
           L+VPV++DAD+KGLSTI +EV+ LAQKA++NSLKPEDYEGGTFTVSNLGGPFGIKQFCA+
Sbjct: 421 LYVPVVKDADRKGLSTIGEEVRLLAQKAKENSLKPEDYEGGTFTVSNLGGPFGIKQFCAV 480

Query: 481 INPPQSGILAVGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYI 539
           +NPPQ+ ILAVGSAEKRV+PG+G ++F FAS+M VTLSCDHRV+DGAIGA+WLKAFKGYI
Sbjct: 481 VNPPQAAILAVGSAEKRVVPGNGPDQFNFASYMPVTLSCDHRVVDGAIGAEWLKAFKGYI 539

BLAST of HG10002354 vs. ExPASy Swiss-Prot
Match: Q8BMF4 (Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial OS=Mus musculus OX=10090 GN=Dlat PE=1 SV=2)

HSP 1 Score: 404.1 bits (1037), Expect = 2.5e-111
Identity = 213/439 (48.52%), Postives = 291/439 (66.29%), Query Frame = 0

Query: 105 SGLPPHQEVGMPSLSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYL 164
           S  P H ++ +P+LSPTMT G + RW KK G+K+S G++L E+ETDKAT+  E  EEGYL
Sbjct: 212 SSYPTHMQIVLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYL 271

Query: 165 AKIICGDGAKEIKVGEVIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVV 224
           AKI+  +G +++ +G  + I VE +EDIA F DY+PT       +    +AP  P     
Sbjct: 272 AKILVPEGTRDVPLGAPLCIIVEKQEDIAAFADYRPTE----VTSLKPQAAPPAPPPVAA 331

Query: 225 EEPVRSPEPKTVKQSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIED 284
             P   P   T   +P  P G R+F SPLA+KLA E  + ++ +KGTGP+G I+K DI+ 
Sbjct: 332 VPPTPQPVAPTPSAAPAGPKG-RVFVSPLAKKLAAEKGIDLTQVKGTGPEGRIIKKDIDS 391

Query: 285 YLASRGKEST----APKAKDAAGAPLD-YSDIPHTQIRKVTASRLLFSKQTIPHYYLTVD 344
           ++ S+   +     AP     A AP   ++DIP + IR+V A RL+ SKQTIPHYYL+VD
Sbjct: 392 FVPSKAAPAAAAAMAPPGPRVAPAPAGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLSVD 451

Query: 345 TCVDKLMDLRNQLNALQEASGAKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNV 404
             + +++ +R +LN + E  G  +ISVND +IKA+ALA  KVP+ NSSW D  IRQ H V
Sbjct: 452 VNMGEVLLVRKELNKMLEGKG--KISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVV 511

Query: 405 NINVAVQTDNGLFVPVIRDADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLG 464
           +++VAV T  GL  P++ +A  KGL TI+ +V  LA KAR+  L+P +++GGTFT+SNL 
Sbjct: 512 DVSVAVSTPAGLITPIVFNAHIKGLETIASDVVSLASKAREGKLQPHEFQGGTFTISNL- 571

Query: 465 GPFGIKQFCAIINPPQSGILAVGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIG 524
           G FGIK F AIINPPQ+ ILA+G++E ++IP    + F  AS MSVTLSCDHRV+DGA+G
Sbjct: 572 GMFGIKNFSAIINPPQACILAIGASEDKLIPADNEKGFDVASVMSVTLSCDHRVVDGAVG 631

Query: 525 ADWLKAFKGYIENPESMLL 539
           A WL  FK Y+E P +MLL
Sbjct: 632 AQWLAEFKKYLEKPITMLL 642

BLAST of HG10002354 vs. ExPASy Swiss-Prot
Match: P08461 (Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial OS=Rattus norvegicus OX=10116 GN=Dlat PE=1 SV=3)

HSP 1 Score: 401.0 bits (1029), Expect = 2.2e-110
Identity = 213/438 (48.63%), Postives = 292/438 (66.67%), Query Frame = 0

Query: 105 SGLPPHQEVGMPSLSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYL 164
           S  P H ++ +P+LSPTMT G + RW KK G+K+S G++L E+ETDKAT+  E  EEGYL
Sbjct: 203 SSYPVHMQIVLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYL 262

Query: 165 AKIICGDGAKEIKVGEVIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVV 224
           AKI+  +G +++ +G  + I VE +EDIA F DY+PT      +   ++  P PP    V
Sbjct: 263 AKILVPEGTRDVPLGTPLCIIVEKQEDIAAFADYRPTEV---TSLKPQAPPPVPPPVAAV 322

Query: 225 EEPVRSPEPKTVKQSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIED 284
             P+  P   T   +P  P G R+F SPLA+KLA E  + ++ +KGTGP+G I+K DI+ 
Sbjct: 323 -PPIPQPLAPTPSAAPAGPKG-RVFVSPLAKKLAAEKGIDLTQVKGTGPEGRIIKKDIDS 382

Query: 285 YL---ASRGKESTAPKAKDAAGAPLD-YSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDT 344
           ++   A+    + AP     A  P   + DIP + IR+V A RL+ SKQTIPHYYL+VD 
Sbjct: 383 FVPTKAAPAAAAAAPPGPRVAPTPAGVFIDIPISNIRRVIAQRLMQSKQTIPHYYLSVDV 442

Query: 345 CVDKLMDLRNQLNALQEASGAKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVN 404
            + +++ +R +LN + E  G  +ISVND +IKA+ALA  KVP+ NSSW D  IRQ H V+
Sbjct: 443 NMGEVLLVRKELNKMLEGKG--KISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVD 502

Query: 405 INVAVQTDNGLFVPVIRDADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGG 464
           ++VAV T  GL  P++ +A  KGL TI+ +V  LA KAR+  L+P +++GGTFT+SNL G
Sbjct: 503 VSVAVSTPAGLITPIVFNAHIKGLETIASDVVSLASKAREGKLQPHEFQGGTFTISNL-G 562

Query: 465 PFGIKQFCAIINPPQSGILAVGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGA 524
            FGIK F AIINPPQ+ ILA+G++E ++IP    + F  AS MSVTLSCDHRV+DGA+GA
Sbjct: 563 MFGIKNFSAIINPPQACILAIGASEDKLIPADNEKGFDVASVMSVTLSCDHRVVDGAVGA 622

Query: 525 DWLKAFKGYIENPESMLL 539
            WL  FK Y+E P +MLL
Sbjct: 623 QWLAEFKKYLEKPVTMLL 632

BLAST of HG10002354 vs. ExPASy Swiss-Prot
Match: P10515 (Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial OS=Homo sapiens OX=9606 GN=DLAT PE=1 SV=3)

HSP 1 Score: 396.4 bits (1017), Expect = 5.3e-109
Identity = 211/441 (47.85%), Postives = 291/441 (65.99%), Query Frame = 0

Query: 105 SGLPPHQEVGMPSLSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYL 164
           S  PPH +V +P+LSPTMT G + RW KK G+K+S G++L E+ETDKAT+  E  EEGYL
Sbjct: 213 SSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYL 272

Query: 165 AKIICGDGAKEIKVGEVIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVV 224
           AKI+  +G +++ +G  + I VE E DI+ F DY+PT          +   PTPP    V
Sbjct: 273 AKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTD---LKPQVPPPTPPPVAAV 332

Query: 225 EEPVRSPEPKTVKQSPPSPAGE--RIFASPLARKLAEENNVPISSIKGTGPDGSIVKADI 284
               +   P      P +PAG   R+F SPLA+KLA E  + ++ +KGTGPDG I K DI
Sbjct: 333 PPTPQPLAPTPSAPCPATPAGPKGRVFVSPLAKKLAVEKGIDLTQVKGTGPDGRITKKDI 392

Query: 285 EDYLASRGKESTA----PKAKDAAGAPLD-YSDIPHTQIRKVTASRLLFSKQTIPHYYLT 344
           + ++ S+   + A    P     A  P   ++DIP + IR+V A RL+ SKQTIPHYYL+
Sbjct: 393 DSFVPSKVAPAPAAVVPPTGPGMAPVPTGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLS 452

Query: 345 VDTCVDKLMDLRNQLNALQEASGAKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYH 404
           +D  + +++ +R +LN + E  G  +ISVND +IKA+ALA  KVP+ NSSW D  IRQ H
Sbjct: 453 IDVNMGEVLLVRKELNKILE--GRSKISVNDFIIKASALACLKVPEANSSWMDTVIRQNH 512

Query: 405 NVNINVAVQTDNGLFVPVIRDADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSN 464
            V+++VAV T  GL  P++ +A  KG+ TI+++V  LA KAR+  L+P +++GGTFT+SN
Sbjct: 513 VVDVSVAVSTPAGLITPIVFNAHIKGVETIANDVVSLATKAREGKLQPHEFQGGTFTISN 572

Query: 465 LGGPFGIKQFCAIINPPQSGILAVGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGA 524
           L G FGIK F AIINPPQ+ ILA+G++E +++P    + F  AS MSVTLSCDHRV+DGA
Sbjct: 573 L-GMFGIKNFSAIINPPQACILAIGASEDKLVPADNEKGFDVASMMSVTLSCDHRVVDGA 632

Query: 525 IGADWLKAFKGYIENPESMLL 539
           +GA WL  F+ Y+E P +MLL
Sbjct: 633 VGAQWLAEFRKYLEKPITMLL 647

BLAST of HG10002354 vs. ExPASy TrEMBL
Match: E5GB89 (Acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 953.0 bits (2462), Expect = 5.4e-274
Identity = 490/538 (91.08%), Postives = 505/538 (93.87%), Query Frame = 0

Query: 1   MAYASQIINHSKKLRSAPKLLRQASVVRWFSEDVESSIVKDNEIWKIQGRGSQASEKGGI 60
           MAYASQIINHSKKLRS PKL RQAS+VRWFSED +SSI  DNEIWKIQG G +AS KGGI
Sbjct: 1   MAYASQIINHSKKLRSTPKLFRQASMVRWFSEDAQSSI--DNEIWKIQGSGYKASGKGGI 60

Query: 61  KNLANFNKDARALILIMIKTRKCLISVFSSNSSRINPMRGFSSDSGLPPHQEVGMPSLSP 120
           KNLANFN  ++ LI     +          + S+INP RGFSSDSGLPPHQEVGMPSLSP
Sbjct: 61  KNLANFNNRSQFLICQRGVSMMATSIGNPFSGSQINPARGFSSDSGLPPHQEVGMPSLSP 120

Query: 121 TMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGE 180
           TMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGE
Sbjct: 121 TMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGE 180

Query: 181 VIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVVEEPVRSPEPKTVKQSP 240
           VIAITVEDEEDIAKFKDYKPTSSNTGAA+A ES AP+PPKKEVVEEPVRSP+P TVKQSP
Sbjct: 181 VIAITVEDEEDIAKFKDYKPTSSNTGAASAPESPAPSPPKKEVVEEPVRSPQPSTVKQSP 240

Query: 241 PSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKD 300
           PSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKD
Sbjct: 241 PSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKD 300

Query: 301 AAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASG 360
           AAGAPLDYSD+PHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASG
Sbjct: 301 AAGAPLDYSDLPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASG 360

Query: 361 AKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDAD 420
            KRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDAD
Sbjct: 361 GKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDAD 420

Query: 421 KKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILA 480
           KKGLSTIS+EVKKLAQKARDN+LKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILA
Sbjct: 421 KKGLSTISNEVKKLAQKARDNTLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILA 480

Query: 481 VGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESMLL 539
           VGSAEKRVIPGSG +EFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESMLL
Sbjct: 481 VGSAEKRVIPGSGAQEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESMLL 536

BLAST of HG10002354 vs. ExPASy TrEMBL
Match: A0A1S3BHA9 (Acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis melo OX=3656 GN=LOC103490036 PE=3 SV=1)

HSP 1 Score: 953.0 bits (2462), Expect = 5.4e-274
Identity = 490/538 (91.08%), Postives = 505/538 (93.87%), Query Frame = 0

Query: 1   MAYASQIINHSKKLRSAPKLLRQASVVRWFSEDVESSIVKDNEIWKIQGRGSQASEKGGI 60
           MAYASQIINHSKKLRS PKL RQAS+VRWFSED +SSI  DNEIWKIQG G +AS KGGI
Sbjct: 1   MAYASQIINHSKKLRSTPKLFRQASMVRWFSEDAQSSI--DNEIWKIQGSGYKASGKGGI 60

Query: 61  KNLANFNKDARALILIMIKTRKCLISVFSSNSSRINPMRGFSSDSGLPPHQEVGMPSLSP 120
           KNLANFN  ++ LI     +          + S+INP RGFSSDSGLPPHQEVGMPSLSP
Sbjct: 61  KNLANFNNRSQFLICQRGVSMMATSIGNPFSGSQINPARGFSSDSGLPPHQEVGMPSLSP 120

Query: 121 TMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGE 180
           TMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGE
Sbjct: 121 TMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIKVGE 180

Query: 181 VIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVVEEPVRSPEPKTVKQSP 240
           VIAITVEDEEDIAKFKDYKPTSSNTGAA+A ES AP+PPKKEVVEEPVRSP+P TVKQSP
Sbjct: 181 VIAITVEDEEDIAKFKDYKPTSSNTGAASAPESPAPSPPKKEVVEEPVRSPQPSTVKQSP 240

Query: 241 PSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKD 300
           PSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKD
Sbjct: 241 PSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPKAKD 300

Query: 301 AAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASG 360
           AAGAPLDYSD+PHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASG
Sbjct: 301 AAGAPLDYSDLPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASG 360

Query: 361 AKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDAD 420
            KRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDAD
Sbjct: 361 GKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIRDAD 420

Query: 421 KKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILA 480
           KKGLSTIS+EVKKLAQKARDN+LKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILA
Sbjct: 421 KKGLSTISNEVKKLAQKARDNTLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILA 480

Query: 481 VGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESMLL 539
           VGSAEKRVIPGSG +EFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESMLL
Sbjct: 481 VGSAEKRVIPGSGAQEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESMLL 536

BLAST of HG10002354 vs. ExPASy TrEMBL
Match: A0A0A0LFR2 (Acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis sativus OX=3659 GN=Csa_3G848140 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 7.1e-274
Identity = 488/541 (90.20%), Postives = 506/541 (93.53%), Query Frame = 0

Query: 1   MAYASQIINHSKKLRSAPKLLRQASVVRWFSEDVESSIVKDNEIWKIQGRGSQASEKGGI 60
           MAYASQIINHSKKLRS PKLLRQASVVRWFSED +SSI KDNEIWKIQG G +AS KGGI
Sbjct: 1   MAYASQIINHSKKLRSTPKLLRQASVVRWFSEDAQSSIAKDNEIWKIQGCGYKASGKGGI 60

Query: 61  KNLANFNKDARALILIMIKTRKCLISVFSSN---SSRINPMRGFSSDSGLPPHQEVGMPS 120
           KNLANFN  ++    +  +    +++    N    S+INP RGFSSDSGLPPHQEVGMPS
Sbjct: 61  KNLANFNNRSQ---FLQCQRGVSMMTTSIGNPFSGSQINPARGFSSDSGLPPHQEVGMPS 120

Query: 121 LSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIK 180
           LSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIK
Sbjct: 121 LSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIK 180

Query: 181 VGEVIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVVEEPVRSPEPKTVK 240
           VGEVIAITVEDEEDIAKFKDYKP SSNTGAA+A ES A +PPKKEVVEEPVRSPEPKTVK
Sbjct: 181 VGEVIAITVEDEEDIAKFKDYKPASSNTGAASAAESPASSPPKKEVVEEPVRSPEPKTVK 240

Query: 241 QSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPK 300
           QSPP PAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPK
Sbjct: 241 QSPPPPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAPK 300

Query: 301 AKDAAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQE 360
           AKDAAGAPLDYSD+PHTQIRK+TASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQE
Sbjct: 301 AKDAAGAPLDYSDLPHTQIRKITASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNALQE 360

Query: 361 ASGAKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIR 420
           ASG KRISVNDLVIKAAALAL+KVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIR
Sbjct: 361 ASGGKRISVNDLVIKAAALALKKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVPVIR 420

Query: 421 DADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSG 480
           DADKKGLS ISDEVKKLAQKARDN+LKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSG
Sbjct: 421 DADKKGLSAISDEVKKLAQKARDNTLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSG 480

Query: 481 ILAVGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESML 539
           ILAVGSAEKRVIPGSG +EFKFASFMSVTLSCDHRVIDGAIGADWLKAFKG+IENPESML
Sbjct: 481 ILAVGSAEKRVIPGSGAQEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGFIENPESML 538

BLAST of HG10002354 vs. ExPASy TrEMBL
Match: A0A6J1IU00 (Acetyltransferase component of pyruvate dehydrogenase complex OS=Cucurbita maxima OX=3661 GN=LOC111479913 PE=3 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 4.8e-262
Identity = 474/544 (87.13%), Postives = 503/544 (92.46%), Query Frame = 0

Query: 1   MAYASQIINHSKKLRSAPKLLRQASVVRWFSEDVESSIVKDNEIWKIQGRGSQASEKGGI 60
           MAY+SQII HSKKLRS PKLLRQAS VRWFSEDV+SSIVK++EIWKI+G G Q+S++G +
Sbjct: 1   MAYSSQIIIHSKKLRSVPKLLRQASAVRWFSEDVQSSIVKEDEIWKIKGCGYQSSQEGDL 60

Query: 61  KNLANFNKDARALIL---IMIKTRKCLISVFSSNSSRINPMRGFSSDSGLPPHQEVGMPS 120
           +NL NFN     L     + + T   + + FSS SS  N  RGF+SDSGLPPHQE+GMPS
Sbjct: 61  QNLVNFNNRPHFLQCKRGLSMMTMTSIGNPFSS-SSPTNSKRGFASDSGLPPHQEIGMPS 120

Query: 121 LSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIK 180
           LSPTMTEGNIARWLKKEG+KISPGEVLCEVETDKATVEMECMEEGYLAKIICGDG KEIK
Sbjct: 121 LSPTMTEGNIARWLKKEGEKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGEKEIK 180

Query: 181 VGEVIAITVEDEEDIAKFKDYKPTSSN--TGAAAATESSAPTPPKKEVVEEPVRSPEPKT 240
           VGEVIAITVEDEEDIAKFKDYKPTSSN   GAAAA ESS P+PPKKE VEEPVRSPE KT
Sbjct: 181 VGEVIAITVEDEEDIAKFKDYKPTSSNADAGAAAAKESSGPSPPKKE-VEEPVRSPESKT 240

Query: 241 VKQSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKES-T 300
           VKQSPP+PAG+RIFASPLARKLAEENNVP+SSIKGTGP+GSIVKADIEDY+ASRGKES T
Sbjct: 241 VKQSPPAPAGDRIFASPLARKLAEENNVPLSSIKGTGPEGSIVKADIEDYMASRGKESTT 300

Query: 301 APKAKDAAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNA 360
           APKAKDAAGAPLDYSD+PHTQIRK+TASRLLFSKQTIPHYYLTVDTCVDKLMDLR QLNA
Sbjct: 301 APKAKDAAGAPLDYSDLPHTQIRKITASRLLFSKQTIPHYYLTVDTCVDKLMDLRKQLNA 360

Query: 361 LQEASGAKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVP 420
           LQEASG KR+SVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVP
Sbjct: 361 LQEASGGKRLSVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVP 420

Query: 421 VIRDADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPP 480
           VIRDADKKGLS+ISDEVKKLAQ+AR+NSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPP
Sbjct: 421 VIRDADKKGLSSISDEVKKLAQRARENSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPP 480

Query: 481 QSGILAVGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPE 539
           QSGILAVGSAEKRVIPGSG EEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPE
Sbjct: 481 QSGILAVGSAEKRVIPGSGAEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPE 540

BLAST of HG10002354 vs. ExPASy TrEMBL
Match: A0A6J1GV94 (Acetyltransferase component of pyruvate dehydrogenase complex OS=Cucurbita moschata OX=3662 GN=LOC111457738 PE=3 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 1.1e-261
Identity = 475/544 (87.32%), Postives = 503/544 (92.46%), Query Frame = 0

Query: 1   MAYASQIINHSKKLRSAPKLLRQASVVRWFSEDVESSIVKDNEIWKIQGRGSQASEKGGI 60
           MAY+SQII HSKKLRS PKLLRQAS VRWFSEDV+SSIVK++EIWKI+GRG Q+S++G I
Sbjct: 1   MAYSSQIIIHSKKLRSVPKLLRQASSVRWFSEDVQSSIVKEDEIWKIKGRGYQSSQEGAI 60

Query: 61  KNLANFNKDARALIL---IMIKTRKCLISVFSSNSSRINPMRGFSSDSGLPPHQEVGMPS 120
           KNL NFN     L     + + T   + + FSS SS  N  RGF+SDSGLPPHQE+GMPS
Sbjct: 61  KNLVNFNHRPHFLQCKRGLSMMTTTSIGNPFSS-SSLTNSKRGFASDSGLPPHQEIGMPS 120

Query: 121 LSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEIK 180
           LSPTMTEGNIARWLKKEG+KISPGEVLCEVETDKATVEMECMEEGYLAKII GDG KEIK
Sbjct: 121 LSPTMTEGNIARWLKKEGEKISPGEVLCEVETDKATVEMECMEEGYLAKIIRGDGEKEIK 180

Query: 181 VGEVIAITVEDEEDIAKFKDYKPTSSN--TGAAAATESSAPTPPKKEVVEEPVRSPEPKT 240
           VGEVIAITVEDEED+AKFKDYKPTSSN   GAAAA ESS P+PPKKE VEEPVRSPE KT
Sbjct: 181 VGEVIAITVEDEEDVAKFKDYKPTSSNADAGAAAAKESSGPSPPKKE-VEEPVRSPESKT 240

Query: 241 VKQSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKES-T 300
           VKQSPP+PAG+RIFASPLARKLAEENNVP+SSIKGTGP+GSIVKADIEDY+ASRGKES T
Sbjct: 241 VKQSPPAPAGDRIFASPLARKLAEENNVPLSSIKGTGPEGSIVKADIEDYMASRGKESTT 300

Query: 301 APKAKDAAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNA 360
           APKAKDAAGAPLDYSD+PHTQIRK+TASRLLFSKQTIPHYYLTVDTCVDKLMDLR QLNA
Sbjct: 301 APKAKDAAGAPLDYSDLPHTQIRKITASRLLFSKQTIPHYYLTVDTCVDKLMDLRKQLNA 360

Query: 361 LQEASGAKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVP 420
           LQEASG KRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVP
Sbjct: 361 LQEASGGKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVP 420

Query: 421 VIRDADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPP 480
           VIRDADKKGLS+IS+EVKKLAQ+AR+NSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPP
Sbjct: 421 VIRDADKKGLSSISEEVKKLAQRARENSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPP 480

Query: 481 QSGILAVGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPE 539
           QSGILAVGSAEKRVIPGSG EEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPE
Sbjct: 481 QSGILAVGSAEKRVIPGSGAEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPE 540

BLAST of HG10002354 vs. TAIR 10
Match: AT3G13930.1 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 708.8 bits (1828), Expect = 3.4e-204
Identity = 378/544 (69.49%), Postives = 439/544 (80.70%), Query Frame = 0

Query: 4   ASQIINHSKKLRSAPKLLRQ--ASVVRWFSEDVESSIVKDNEIWKIQGRGSQASE----- 63
           AS+IINHSKKL+    LLR+  A  VR FS     S+V   +I+K +   S         
Sbjct: 2   ASRIINHSKKLKHVSALLRRDHAVAVRCFSNSTHPSLVGREDIFKARLNYSSVERISKCG 61

Query: 64  KGGIKNLANFNKDARALILIMIKTRKCLISVFSSNSSRINPMRGFSSDSGLPPHQEVGMP 123
            G +  L+  +  +  L   M   +  L   F   SS++  +RGFSS S LPPHQE+GMP
Sbjct: 62  TGNVTMLSGISTTSTKLSSPMAGPK--LFKEFI--SSQMRSVRGFSSSSDLPPHQEIGMP 121

Query: 124 SLSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDGAKEI 183
           SLSPTMTEGNIARWLKKEGDK++PGEVLCEVETDKATVEMECMEEG+LAKI+  +GAKEI
Sbjct: 122 SLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGFLAKIVKEEGAKEI 181

Query: 184 KVGEVIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVVEEPVRSPEPKTV 243
           +VGEVIAITVEDE+DI KFKDY P SS+TG AA     AP+ PK+E VE+P  +PE K  
Sbjct: 182 QVGEVIAITVEDEDDIQKFKDYTP-SSDTGPAAPEAKPAPSLPKEEKVEKPASAPEAKIS 241

Query: 244 KQSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKESTAP 303
           K S  +P+ +RIFASPLARKLAE+NNVP+SSIKGTGP+G IVKAD+ED+LAS  KE+TA 
Sbjct: 242 KPS-SAPSEDRIFASPLARKLAEDNNVPLSSIKGTGPEGRIVKADVEDFLASGSKETTAK 301

Query: 304 KAK--DAAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRNQLNA 363
            +K  D+    LDY DIPHTQIRKVTASRL FSKQTIPHYYLTVDTCVDK+M LR+QLN+
Sbjct: 302 PSKQVDSKVPALDYVDIPHTQIRKVTASRLAFSKQTIPHYYLTVDTCVDKMMGLRSQLNS 361

Query: 364 LQEASGAKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNGLFVP 423
            QEASG KRISVNDLVIKAAALALRKVPQCNSSWTD YIRQ+ NVNINVAVQT+NGL+VP
Sbjct: 362 FQEASGGKRISVNDLVIKAAALALRKVPQCNSSWTDEYIRQFKNVNINVAVQTENGLYVP 421

Query: 424 VIRDADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAIINPP 483
           V++DADKKGLSTI +EV+ LAQKA++NSLKPEDYEGGTFTVSNLGGPFGIKQFCA+INPP
Sbjct: 422 VVKDADKKGLSTIGEEVRFLAQKAKENSLKPEDYEGGTFTVSNLGGPFGIKQFCAVINPP 481

Query: 484 QSGILAVGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYIENPE 539
           Q+ ILA+GSAEKRV+PG+G +++  AS+MSVTLSCDHRVIDGAIGA+WLKAFKGYIE PE
Sbjct: 482 QAAILAIGSAEKRVVPGTGPDQYNVASYMSVTLSCDHRVIDGAIGAEWLKAFKGYIETPE 539

BLAST of HG10002354 vs. TAIR 10
Match: AT1G54220.1 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 706.1 bits (1821), Expect = 2.2e-203
Identity = 369/548 (67.34%), Postives = 438/548 (79.93%), Query Frame = 0

Query: 1   MAYASQIINHSKKLRSAPKLLRQ--ASVVRWFSEDVESSIVKDNEIWKIQGRGSQASEKG 60
           MAYAS+IINHSKKL+    LLR+  A+ +R++S    + + +++      G         
Sbjct: 1   MAYASRIINHSKKLKDVSTLLRRENAATIRYYSNTNRAPLNREDTFNSRLGYPP------ 60

Query: 61  GIKNLANFNKDARALILIMIKTRKCLIS-----VFSSN-SSRINPMRGFSSDSGLPPHQE 120
            ++ ++  +     + +I   TR  L S     +F    S  +   RGFSS S LPPHQE
Sbjct: 61  -LERISICSTSTLPVSIIFSTTRSNLSSAMGRPIFGKEFSCLMQSARGFSSGSDLPPHQE 120

Query: 121 VGMPSLSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDG 180
           +GMPSLSPTMTEGNIARWLKKEGDK++PGEVLCEVETDKATVEMECMEEGYLAKI+  +G
Sbjct: 121 IGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYLAKIVKAEG 180

Query: 181 AKEIKVGEVIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVVEEPVRSPE 240
           +KEI+VGEVIAITVEDEEDI KFKDY P SS   AA       P PPK+E V++P   PE
Sbjct: 181 SKEIQVGEVIAITVEDEEDIGKFKDYTP-SSTADAAPTKAEPTPAPPKEEKVKQPSSPPE 240

Query: 241 PKTVKQSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKE 300
           PK  K S P P G+R+FASPLARKLAE+NNVP+S I+GTGP+G IVKADI++YLAS GK 
Sbjct: 241 PKASKPSTP-PTGDRVFASPLARKLAEDNNVPLSDIEGTGPEGRIVKADIDEYLASSGKG 300

Query: 301 STA--PKAKDAAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRN 360
           +TA   K+ D+    LDY DIPH+QIRKVTASRL FSKQTIPHYYLTVDTCVDKLM LR+
Sbjct: 301 ATAKPSKSTDSKAPALDYVDIPHSQIRKVTASRLAFSKQTIPHYYLTVDTCVDKLMALRS 360

Query: 361 QLNALQEASGAKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNG 420
           QLN+ +EASG KRISVNDLV+KAAALALRKVPQCNSSWTD+YIRQ+ NVNINVAVQT+NG
Sbjct: 361 QLNSFKEASGGKRISVNDLVVKAAALALRKVPQCNSSWTDDYIRQFKNVNINVAVQTENG 420

Query: 421 LFVPVIRDADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAI 480
           L+VPV++DAD+KGLSTI +EV+ LAQKA++NSLKPEDYEGGTFTVSNLGGPFGIKQFCA+
Sbjct: 421 LYVPVVKDADRKGLSTIGEEVRLLAQKAKENSLKPEDYEGGTFTVSNLGGPFGIKQFCAV 480

Query: 481 INPPQSGILAVGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYI 539
           +NPPQ+ ILAVGSAEKRV+PG+G ++F FAS+M VTLSCDHRV+DGAIGA+WLKAFKGYI
Sbjct: 481 VNPPQAAILAVGSAEKRVVPGNGPDQFNFASYMPVTLSCDHRVVDGAIGAEWLKAFKGYI 539

BLAST of HG10002354 vs. TAIR 10
Match: AT1G54220.2 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 706.1 bits (1821), Expect = 2.2e-203
Identity = 369/548 (67.34%), Postives = 438/548 (79.93%), Query Frame = 0

Query: 1   MAYASQIINHSKKLRSAPKLLRQ--ASVVRWFSEDVESSIVKDNEIWKIQGRGSQASEKG 60
           MAYAS+IINHSKKL+    LLR+  A+ +R++S    + + +++      G         
Sbjct: 1   MAYASRIINHSKKLKDVSTLLRRENAATIRYYSNTNRAPLNREDTFNSRLGYPP------ 60

Query: 61  GIKNLANFNKDARALILIMIKTRKCLIS-----VFSSN-SSRINPMRGFSSDSGLPPHQE 120
            ++ ++  +     + +I   TR  L S     +F    S  +   RGFSS S LPPHQE
Sbjct: 61  -LERISICSTSTLPVSIIFSTTRSNLSSAMGRPIFGKEFSCLMQSARGFSSGSDLPPHQE 120

Query: 121 VGMPSLSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYLAKIICGDG 180
           +GMPSLSPTMTEGNIARWLKKEGDK++PGEVLCEVETDKATVEMECMEEGYLAKI+  +G
Sbjct: 121 IGMPSLSPTMTEGNIARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYLAKIVKAEG 180

Query: 181 AKEIKVGEVIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVVEEPVRSPE 240
           +KEI+VGEVIAITVEDEEDI KFKDY P SS   AA       P PPK+E V++P   PE
Sbjct: 181 SKEIQVGEVIAITVEDEEDIGKFKDYTP-SSTADAAPTKAEPTPAPPKEEKVKQPSSPPE 240

Query: 241 PKTVKQSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIEDYLASRGKE 300
           PK  K S P P G+R+FASPLARKLAE+NNVP+S I+GTGP+G IVKADI++YLAS GK 
Sbjct: 241 PKASKPSTP-PTGDRVFASPLARKLAEDNNVPLSDIEGTGPEGRIVKADIDEYLASSGKG 300

Query: 301 STA--PKAKDAAGAPLDYSDIPHTQIRKVTASRLLFSKQTIPHYYLTVDTCVDKLMDLRN 360
           +TA   K+ D+    LDY DIPH+QIRKVTASRL FSKQTIPHYYLTVDTCVDKLM LR+
Sbjct: 301 ATAKPSKSTDSKAPALDYVDIPHSQIRKVTASRLAFSKQTIPHYYLTVDTCVDKLMALRS 360

Query: 361 QLNALQEASGAKRISVNDLVIKAAALALRKVPQCNSSWTDNYIRQYHNVNINVAVQTDNG 420
           QLN+ +EASG KRISVNDLV+KAAALALRKVPQCNSSWTD+YIRQ+ NVNINVAVQT+NG
Sbjct: 361 QLNSFKEASGGKRISVNDLVVKAAALALRKVPQCNSSWTDDYIRQFKNVNINVAVQTENG 420

Query: 421 LFVPVIRDADKKGLSTISDEVKKLAQKARDNSLKPEDYEGGTFTVSNLGGPFGIKQFCAI 480
           L+VPV++DAD+KGLSTI +EV+ LAQKA++NSLKPEDYEGGTFTVSNLGGPFGIKQFCA+
Sbjct: 421 LYVPVVKDADRKGLSTIGEEVRLLAQKAKENSLKPEDYEGGTFTVSNLGGPFGIKQFCAV 480

Query: 481 INPPQSGILAVGSAEKRVIPGSGTEEFKFASFMSVTLSCDHRVIDGAIGADWLKAFKGYI 539
           +NPPQ+ ILAVGSAEKRV+PG+G ++F FAS+M VTLSCDHRV+DGAIGA+WLKAFKGYI
Sbjct: 481 VNPPQAAILAVGSAEKRVVPGNGPDQFNFASYMPVTLSCDHRVVDGAIGAEWLKAFKGYI 539

BLAST of HG10002354 vs. TAIR 10
Match: AT3G52200.1 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 316.2 bits (809), Expect = 5.0e-86
Identity = 198/457 (43.33%), Postives = 272/457 (59.52%), Query Frame = 0

Query: 105 SGLPPHQEVGMPSLSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYL 164
           S LPPH  + MP+LSPTM +GNIA+W KKEGDKI  G+V+ E+ETDKAT+E E +EEGYL
Sbjct: 207 SDLPPHVVLEMPALSPTMNQGNIAKWWKKEGDKIEVGDVIGEIETDKATLEFESLEEGYL 266

Query: 165 AKIICGDGAKEIKVGEVIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVV 224
           AKI+  +G+K++ VG+ IA+ VED E I   K     SS+ G       S+     KEV 
Sbjct: 267 AKILIPEGSKDVAVGKPIALIVEDAESIEAIK-----SSSAG-------SSEVDTVKEVP 326

Query: 225 EEPVRSPEPKTVKQSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIED 284
           +  V  P  +    +  SPA         A+ L  E+ +  SSI+ +GP G+++K+D+  
Sbjct: 327 DSVVDKPTERKAGFTKISPA---------AKLLILEHGLEASSIEASGPYGTLLKSDVVA 386

Query: 285 YLAS--RGKESTAPKAK----------------DAAGAPLDYSDIPHTQIRKVTASRLLF 344
            +AS    K S + K K                    +  +Y D P++QIRK+ A RLL 
Sbjct: 387 AIASGKASKSSASTKKKQPSKETPSKSSSTSKPSVTQSDNNYEDFPNSQIRKIIAKRLLE 446

Query: 345 SKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASGAKRISVNDLVIKAAALALRKVPQCNS 404
           SKQ IPH YL  D  +D L+  R +   LQE  G K +SVND+VIKA A+ALR V Q N+
Sbjct: 447 SKQKIPHLYLQSDVVLDPLLAFRKE---LQENHGVK-VSVNDIVIKAVAVALRNVRQANA 506

Query: 405 SW--TDNYIRQYHNVNINVAVQTDNGLFVPVIRDADKKGLSTISDEVKKLAQKARDNSLK 464
            W      I    +V+I++AV T+ GL  P+I++AD+K +S IS EVK+LAQKAR   L 
Sbjct: 507 FWDAEKGDIVMCDSVDISIAVATEKGLMTPIIKNADQKSISAISLEVKELAQKARSGKLA 566

Query: 465 PEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILAVGSAEKRVIPG---SGTEEFKFAS 524
           P +++GGTF++SNL G + +  FCAIINPPQ+GILAVG   K V P     G E+    +
Sbjct: 567 PHEFQGGTFSISNL-GMYPVDNFCAIINPPQAGILAVGRGNKVVEPVIGLDGIEKPSVVT 626

Query: 525 FMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESMLL 539
            M+VTLS DHR+ DG +GA ++   +   E+   +LL
Sbjct: 627 KMNVTLSADHRIFDGQVGASFMSELRSNFEDVRRLLL 637

BLAST of HG10002354 vs. TAIR 10
Match: AT3G52200.2 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 316.2 bits (809), Expect = 5.0e-86
Identity = 198/457 (43.33%), Postives = 272/457 (59.52%), Query Frame = 0

Query: 105 SGLPPHQEVGMPSLSPTMTEGNIARWLKKEGDKISPGEVLCEVETDKATVEMECMEEGYL 164
           S LPPH  + MP+LSPTM +GNIA+W KKEGDKI  G+V+ E+ETDKAT+E E +EEGYL
Sbjct: 283 SDLPPHVVLEMPALSPTMNQGNIAKWWKKEGDKIEVGDVIGEIETDKATLEFESLEEGYL 342

Query: 165 AKIICGDGAKEIKVGEVIAITVEDEEDIAKFKDYKPTSSNTGAAAATESSAPTPPKKEVV 224
           AKI+  +G+K++ VG+ IA+ VED E I   K     SS+ G       S+     KEV 
Sbjct: 343 AKILIPEGSKDVAVGKPIALIVEDAESIEAIK-----SSSAG-------SSEVDTVKEVP 402

Query: 225 EEPVRSPEPKTVKQSPPSPAGERIFASPLARKLAEENNVPISSIKGTGPDGSIVKADIED 284
           +  V  P  +    +  SPA         A+ L  E+ +  SSI+ +GP G+++K+D+  
Sbjct: 403 DSVVDKPTERKAGFTKISPA---------AKLLILEHGLEASSIEASGPYGTLLKSDVVA 462

Query: 285 YLAS--RGKESTAPKAK----------------DAAGAPLDYSDIPHTQIRKVTASRLLF 344
            +AS    K S + K K                    +  +Y D P++QIRK+ A RLL 
Sbjct: 463 AIASGKASKSSASTKKKQPSKETPSKSSSTSKPSVTQSDNNYEDFPNSQIRKIIAKRLLE 522

Query: 345 SKQTIPHYYLTVDTCVDKLMDLRNQLNALQEASGAKRISVNDLVIKAAALALRKVPQCNS 404
           SKQ IPH YL  D  +D L+  R +   LQE  G K +SVND+VIKA A+ALR V Q N+
Sbjct: 523 SKQKIPHLYLQSDVVLDPLLAFRKE---LQENHGVK-VSVNDIVIKAVAVALRNVRQANA 582

Query: 405 SW--TDNYIRQYHNVNINVAVQTDNGLFVPVIRDADKKGLSTISDEVKKLAQKARDNSLK 464
            W      I    +V+I++AV T+ GL  P+I++AD+K +S IS EVK+LAQKAR   L 
Sbjct: 583 FWDAEKGDIVMCDSVDISIAVATEKGLMTPIIKNADQKSISAISLEVKELAQKARSGKLA 642

Query: 465 PEDYEGGTFTVSNLGGPFGIKQFCAIINPPQSGILAVGSAEKRVIPG---SGTEEFKFAS 524
           P +++GGTF++SNL G + +  FCAIINPPQ+GILAVG   K V P     G E+    +
Sbjct: 643 PHEFQGGTFSISNL-GMYPVDNFCAIINPPQAGILAVGRGNKVVEPVIGLDGIEKPSVVT 702

Query: 525 FMSVTLSCDHRVIDGAIGADWLKAFKGYIENPESMLL 539
            M+VTLS DHR+ DG +GA ++   +   E+   +LL
Sbjct: 703 KMNVTLSADHRIFDGQVGASFMSELRSNFEDVRRLLL 713

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038904277.12.6e-27892.42dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogen... [more]
XP_008447623.11.1e-27391.08PREDICTED: dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate... [more]
XP_004146813.11.5e-27390.20dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogen... [more]
KGN59926.21.9e-26885.02hypothetical protein Csa_002187 [Cucumis sativus][more]
XP_031739329.12.3e-26689.79dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogen... [more]
Match NameE-valueIdentityDescription
Q8RWN94.8e-20369.49Dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogen... [more]
Q5M7293.1e-20267.34Dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogen... [more]
Q8BMF42.5e-11148.52Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenas... [more]
P084612.2e-11048.63Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenas... [more]
P105155.3e-10947.85Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenas... [more]
Match NameE-valueIdentityDescription
E5GB895.4e-27491.08Acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis melo su... [more]
A0A1S3BHA95.4e-27491.08Acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis melo OX... [more]
A0A0A0LFR27.1e-27490.20Acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis sativus... [more]
A0A6J1IU004.8e-26287.13Acetyltransferase component of pyruvate dehydrogenase complex OS=Cucurbita maxim... [more]
A0A6J1GV941.1e-26187.32Acetyltransferase component of pyruvate dehydrogenase complex OS=Cucurbita mosch... [more]
Match NameE-valueIdentityDescription
AT3G13930.13.4e-20469.49Dihydrolipoamide acetyltransferase, long form protein [more]
AT1G54220.12.2e-20367.34Dihydrolipoamide acetyltransferase, long form protein [more]
AT1G54220.22.2e-20367.34Dihydrolipoamide acetyltransferase, long form protein [more]
AT3G52200.15.0e-8643.33Dihydrolipoamide acetyltransferase, long form protein [more]
AT3G52200.25.0e-8643.33Dihydrolipoamide acetyltransferase, long form protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 338..358
NoneNo IPR availableGENE3D2.40.50.100coord: 105..211
e-value: 1.1E-35
score: 123.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 198..212
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 197..245
NoneNo IPR availablePANTHERPTHR43178DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEXcoord: 81..538
NoneNo IPR availablePANTHERPTHR43178:SF1DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT 3 OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIALcoord: 81..538
NoneNo IPR availableCDDcd06849lipoyl_domaincoord: 111..184
e-value: 2.05414E-29
score: 108.646
NoneNo IPR availableSUPERFAMILY52777CoA-dependent acyltransferasescoord: 307..538
IPR000089Biotin/lipoyl attachmentPFAMPF00364Biotin_lipoylcoord: 112..184
e-value: 1.3E-18
score: 66.6
IPR000089Biotin/lipoyl attachmentPROSITEPS50968BIOTINYL_LIPOYLcoord: 110..186
score: 26.331142
IPR004167Peripheral subunit-binding domainPFAMPF02817E3_bindingcoord: 248..283
e-value: 4.6E-15
score: 55.7
IPR004167Peripheral subunit-binding domainPROSITEPS51826PSBDcoord: 249..286
score: 20.653009
IPR006257Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complexTIGRFAMTIGR01349TIGR01349coord: 113..538
e-value: 1.1E-166
score: 553.3
IPR036625E3-binding domain superfamilyGENE3D4.10.320.10coord: 243..292
e-value: 7.2E-18
score: 65.9
IPR036625E3-binding domain superfamilySUPERFAMILY47005Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complexcoord: 242..287
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 293..536
e-value: 2.3E-90
score: 304.4
IPR0010782-oxoacid dehydrogenase acyltransferase, catalytic domainPFAMPF001982-oxoacid_dhcoord: 308..538
e-value: 3.0E-79
score: 265.7
IPR0030162-oxo acid dehydrogenase, lipoyl-binding sitePROSITEPS00189LIPOYLcoord: 135..164
IPR011053Single hybrid motifSUPERFAMILY51230Single hybrid motifcoord: 110..203

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10002354.1HG10002354.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006887 exocytosis
biological_process GO:0006893 Golgi to plasma membrane transport
biological_process GO:0006090 pyruvate metabolic process
cellular_component GO:0000145 exocyst
cellular_component GO:0005759 mitochondrial matrix
cellular_component GO:0005886 plasma membrane
cellular_component GO:0045254 pyruvate dehydrogenase complex
molecular_function GO:0004742 dihydrolipoyllysine-residue acetyltransferase activity
molecular_function GO:0031405 lipoic acid binding
molecular_function GO:0005546 phosphatidylinositol-4,5-bisphosphate binding
molecular_function GO:0016746 acyltransferase activity