HG10002222 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10002222
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	26.5 kDa heat shock protein
Location	Chr11: 4650890 .. 4651516 (-)
RNA-Seq Expression	HG10002222
Synteny	HG10002222

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCTGACCCGTTTGGCTCTGAAGAATTTGCAGCAAAGGGCCCTTGTTTCCTCTCCTGCTGATGCTCTGCTCCCCAGGCAAACTGAATCCCTTATCAGAAGGTTGTGTGAACAGAGCTCAGAAGGCAATCCCAAAGAGGTCGCCGTTAATAATGAAGACAGAAACACCAAAATAGTCCCGAAAAGGGAAGGCAAGAAGTGGGTACCATGGAGGAAGAATGGGGGAGAGTTTCATCTGGGAAATTCACTGATGCAAGCGGCAGAGAACCTAAACAGGGTGTTGAAGAGCTTGAACTTGAGTCGTCCATGGTGGGTCTCCGGTGGCCATGTGAAAGAGCAAGAAGATTGGTACAAGCTGCGAGTGGAGATGCCTGGGATTGCCAAGGAAGAGGTGAAGGTGACAGTGGAGGGTCGGATTCTGAGCATCAGAGGAGAGCACAGGGAAGAAGAGGAAGAAGAAGAAGAATTTGCTGAGAGTTATGGTTACTATGAAAGCACGGTGATGCTGCCGGAAGATGCGGTGGCGGATGAGATCAAGGCGGAGTTGAAAGATGGAGTTTTGACGATCACCATTCCGAGGACTGAGAAGCCCCCAAAGGATGTTAAAGAGATTACTGTTGCGTGA

mRNA sequence

ATGGCTCTGACCCGTTTGGCTCTGAAGAATTTGCAGCAAAGGGCCCTTGTTTCCTCTCCTGCTGATGCTCTGCTCCCCAGGCAAACTGAATCCCTTATCAGAAGGTTGTGTGAACAGAGCTCAGAAGGCAATCCCAAAGAGGTCGCCGTTAATAATGAAGACAGAAACACCAAAATAGTCCCGAAAAGGGAAGGCAAGAAGTGGGTACCATGGAGGAAGAATGGGGGAGAGTTTCATCTGGGAAATTCACTGATGCAAGCGGCAGAGAACCTAAACAGGGTGTTGAAGAGCTTGAACTTGAGTCGTCCATGGTGGGTCTCCGGTGGCCATGTGAAAGAGCAAGAAGATTGGTACAAGCTGCGAGTGGAGATGCCTGGGATTGCCAAGGAAGAGGTGAAGGTGACAGTGGAGGGTCGGATTCTGAGCATCAGAGGAGAGCACAGGGAAGAAGAGGAAGAAGAAGAAGAATTTGCTGAGAGTTATGGTTACTATGAAAGCACGGTGATGCTGCCGGAAGATGCGGTGGCGGATGAGATCAAGGCGGAGTTGAAAGATGGAGTTTTGACGATCACCATTCCGAGGACTGAGAAGCCCCCAAAGGATGTTAAAGAGATTACTGTTGCGTGA

Coding sequence (CDS)

ATGGCTCTGACCCGTTTGGCTCTGAAGAATTTGCAGCAAAGGGCCCTTGTTTCCTCTCCTGCTGATGCTCTGCTCCCCAGGCAAACTGAATCCCTTATCAGAAGGTTGTGTGAACAGAGCTCAGAAGGCAATCCCAAAGAGGTCGCCGTTAATAATGAAGACAGAAACACCAAAATAGTCCCGAAAAGGGAAGGCAAGAAGTGGGTACCATGGAGGAAGAATGGGGGAGAGTTTCATCTGGGAAATTCACTGATGCAAGCGGCAGAGAACCTAAACAGGGTGTTGAAGAGCTTGAACTTGAGTCGTCCATGGTGGGTCTCCGGTGGCCATGTGAAAGAGCAAGAAGATTGGTACAAGCTGCGAGTGGAGATGCCTGGGATTGCCAAGGAAGAGGTGAAGGTGACAGTGGAGGGTCGGATTCTGAGCATCAGAGGAGAGCACAGGGAAGAAGAGGAAGAAGAAGAAGAATTTGCTGAGAGTTATGGTTACTATGAAAGCACGGTGATGCTGCCGGAAGATGCGGTGGCGGATGAGATCAAGGCGGAGTTGAAAGATGGAGTTTTGACGATCACCATTCCGAGGACTGAGAAGCCCCCAAAGGATGTTAAAGAGATTACTGTTGCGTGA

Protein sequence

MALTRLALKNLQQRALVSSPADALLPRQTESLIRRLCEQSSEGNPKEVAVNNEDRNTKIVPKREGKKWVPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGGHVKEQEDWYKLRVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEEFAESYGYYESTVMLPEDAVADEIKAELKDGVLTITIPRTEKPPKDVKEITVA

Homology

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BLAST of HG10002222 vs. NCBI nr
Match: XP_038899919.1 (26.5 kDa heat shock protein, mitochondrial [Benincasa hispida])

HSP 1 Score: 372.1 bits (954), Expect = 3.2e-99
Identity = 192/208 (92.31%), Postives = 198/208 (95.19%), Query Frame = 0


Query: 1   MALTRLALKNLQQR-ALVSSPADALLPRQTESLIRRLCEQSSEGNPKEVAVNNEDRNTKI 60
           MALTRLALKNLQQR ALVSSPADALLPR+TE+LIRRLC+QSSE NPKE+ V +EDRN KI
Sbjct: 1   MALTRLALKNLQQRAALVSSPADALLPRRTEALIRRLCDQSSEVNPKEITVKDEDRNPKI 60

Query: 61  VPKREGKKWVPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGGHVKEQEDWYK 120
           VPKRE KKW PWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGG VKEQEDWYK
Sbjct: 61  VPKRESKKWAPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGGRVKEQEDWYK 120

Query: 121 LRVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEEFAESYGYYESTVMLPEDAVADEI 180
           LRVEMPGIAKEEVKVTVEGRIL+IRGEHREEEEEEEEFAESYGYYESTVMLPEDAVADEI
Sbjct: 121 LRVEMPGIAKEEVKVTVEGRILNIRGEHREEEEEEEEFAESYGYYESTVMLPEDAVADEI 180

Query: 181 KAELKDGVLTITIPRTEKPPKDVKEITV 208
           KAELKDGVLTITIPR EKP KDVKEITV
Sbjct: 181 KAELKDGVLTITIPRAEKPSKDVKEITV 208

[Close]

BLAST of HG10002222 vs. NCBI nr
Match: XP_008454491.1 (PREDICTED: 26.5 kDa heat shock protein, mitochondrial [Cucumis melo] >KAA0044442.1 26.5 kDa heat shock protein [Cucumis melo var. makuwa] >TYK29569.1 26.5 kDa heat shock protein [Cucumis melo var. makuwa])

HSP 1 Score: 358.6 bits (919), Expect = 3.6e-95
Identity = 182/210 (86.67%), Postives = 197/210 (93.81%), Query Frame = 0


Query: 1   MALTRLALKNLQQR-ALVSSPADALLPRQTESLIRRLCEQSSEGNPKEVAVNNEDRNTKI 60
           MALTRLAL+NLQQR A VSSP D LLP+QT +LIRR C+QSSEGN KE+AV NE++NTKI
Sbjct: 1   MALTRLALRNLQQRAAFVSSPVDTLLPKQTAALIRRFCDQSSEGNSKEIAVKNEEKNTKI 60

Query: 61  VPKREGKKWVPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGGHVKEQEDWYK 120
            PKREGKKW+PWRKN GEFHLGNSLMQAAE++NRVLKSLNLSRPWW SGGHVKEQEDWYK
Sbjct: 61  APKREGKKWIPWRKNEGEFHLGNSLMQAAEHINRVLKSLNLSRPWWFSGGHVKEQEDWYK 120

Query: 121 LRVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEE--FAESYGYYESTVMLPEDAVAD 180
           LR+EMPGIAK+EVKVTVEGR+L+IRGEHREEEEEEEE  FAESYGYYESTVMLPEDAVA+
Sbjct: 121 LRMEMPGIAKDEVKVTVEGRMLNIRGEHREEEEEEEEEGFAESYGYYESTVMLPEDAVAE 180

Query: 181 EIKAELKDGVLTITIPRTEKPPKDVKEITV 208
           EIKAELKDGVLTITIPRTEKPPKDVKEITV
Sbjct: 181 EIKAELKDGVLTITIPRTEKPPKDVKEITV 210

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BLAST of HG10002222 vs. NCBI nr
Match: XP_004152349.1 (26.5 kDa heat shock protein, mitochondrial [Cucumis sativus] >KGN52891.1 hypothetical protein Csa_014496 [Cucumis sativus])

HSP 1 Score: 347.1 bits (889), Expect = 1.1e-91
Identity = 181/210 (86.19%), Postives = 194/210 (92.38%), Query Frame = 0


Query: 1   MALTRLALKNLQQR-ALVSSPADALLPRQTESLIRRLCEQSSEGNPKEVAVNNEDRNTKI 60
           MALTRLAL+NLQQR A  SSP D L P+QT +LIRRL +QSSEGN KE+AV NE+ NTKI
Sbjct: 1   MALTRLALRNLQQRAAFASSPVDTLFPKQTAALIRRLSDQSSEGNSKEIAVKNEE-NTKI 60

Query: 61  VPKREGKKWVPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGGHVKEQEDWYK 120
            PKREGKKWVPWRKN GEFHLGNSLMQAAE++NRVLKSLNLSRPWWVSGG VKEQEDWYK
Sbjct: 61  APKREGKKWVPWRKNEGEFHLGNSLMQAAEHINRVLKSLNLSRPWWVSGGRVKEQEDWYK 120

Query: 121 LRVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEE--FAESYGYYESTVMLPEDAVAD 180
           LRVEMPGIAK+EVKVTVEGR+L+IRGEHREEEEEEE+  FAESYGYYESTVMLPEDAVA+
Sbjct: 121 LRVEMPGIAKDEVKVTVEGRMLNIRGEHREEEEEEEDEGFAESYGYYESTVMLPEDAVAE 180

Query: 181 EIKAELKDGVLTITIPRTEKPPKDVKEITV 208
           EIKAELKDGVLTITIPRTEKPPKDVKEITV
Sbjct: 181 EIKAELKDGVLTITIPRTEKPPKDVKEITV 209

[Close]

BLAST of HG10002222 vs. NCBI nr
Match: KAG6581923.1 (26.5 kDa heat shock protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 344.4 bits (882), Expect = 7.1e-91
Identity = 178/209 (85.17%), Postives = 190/209 (90.91%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADALLPRQTESLIRRLCEQSSEGNPKEVAVNNEDRNTKIV 60
           MALTRLALKNLQQRALVSS ADALL RQTE+LIRR C+QSSEGNPKEVA+NNED+NTK+V
Sbjct: 80  MALTRLALKNLQQRALVSSSADALLHRQTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 139

Query: 61  PKREGKKWVPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGGHVKEQEDWYKL 120
           PK EGKKWV W+KNG EF +GNSLMQAAEN+NRVLKSL L RPWWVS GHVKEQE+WYKL
Sbjct: 140 PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSNGHVKEQEEWYKL 199

Query: 121 RVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEE--FAESYGYYESTVMLPEDAVADE 180
           +VEMPGI KE+VKV VEGRILSIRGEHREE+EEEE     E YGYYESTVMLPEDAVADE
Sbjct: 200 KVEMPGIRKEDVKVRVEGRILSIRGEHREEDEEEESGWRGERYGYYESTVMLPEDAVADE 259

Query: 181 IKAELKDGVLTITIPRTEKPPKDVKEITV 208
           IKAELKDGVLTITIPRTE PPKDVKEITV
Sbjct: 260 IKAELKDGVLTITIPRTEMPPKDVKEITV 288

[Close]

BLAST of HG10002222 vs. NCBI nr
Match: XP_022979416.1 (26.5 kDa heat shock protein, mitochondrial [Cucurbita maxima])

HSP 1 Score: 344.4 bits (882), Expect = 7.1e-91
Identity = 177/209 (84.69%), Postives = 190/209 (90.91%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADALLPRQTESLIRRLCEQSSEGNPKEVAVNNEDRNTKIV 60
           MALTRLALKNLQQRALVSSPAD+LL R+TE+LIRR C+QSSEGNPKEVA+NNED+NTK+V
Sbjct: 1   MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 60

Query: 61  PKREGKKWVPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGGHVKEQEDWYKL 120
           PK EGKKWV W+KNG EF +GNSLMQAAEN+NRVLKSL L RPWWVS GHVKEQE+WYKL
Sbjct: 61  PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKL 120

Query: 121 RVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEE--FAESYGYYESTVMLPEDAVADE 180
           RVEMPGI KE+VKV VEGRILSIRGEH EE+EEEE     E YGYYESTVMLPEDAVADE
Sbjct: 121 RVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADE 180

Query: 181 IKAELKDGVLTITIPRTEKPPKDVKEITV 208
           IKAELKDGVLTITIPRTE PPKDVKEITV
Sbjct: 181 IKAELKDGVLTITIPRTEMPPKDVKEITV 209

[Close]

BLAST of HG10002222 vs. ExPASy Swiss-Prot
Match: Q9SSQ8 (26.5 kDa heat shock protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HSP26.5 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.1e-33
Identity = 96/234 (41.03%), Postives = 146/234 (62.39%), Query Frame = 0


Query: 1   MALTRLALKNLQQR---ALVSSPADALL------PRQTESLIRRLCEQSSEGNPKEVAVN 60
           MAL RLAL+NLQQ+   +L+    +  L      P +    +     +  +    EV+V+
Sbjct: 1   MALARLALRNLQQKLSPSLMGQSCERGLVGNRHNPMKLNRFMATSAGEQEDKMNTEVSVS 60

Query: 61  NEDRNTKIVPKREGKKWVPWRKNG---------GEFH---LGNSLMQAAENLNRVLKSLN 120
            +    +  P+R G+K + WR             EF    +GN+L+QA EN+NR+  + N
Sbjct: 61  EKKSPRQNFPRRRGRKSL-WRNTDDHGYFTPTLNEFFPPTIGNTLIQATENMNRIFDNFN 120

Query: 121 LSRPWWVSGGHVKEQEDWYKLRVEMPGIAKEEVKVTVEGRILSIRGEHREEEE----EEE 180
           ++ P+ +  G VKEQ+D YKLR E+PG+ KE+VK+TV   IL+I+G+H+ EEE    EE+
Sbjct: 121 VN-PFQLM-GQVKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEED 180

Query: 181 EF--AESYGYYESTVMLPEDAVADEIKAELKDGVLTITIPRTEKPPKDVKEITV 208
           E+  ++SYGYY +++ LP+DA  ++IKAELK+GVL + IPRTEKP K+V+EI+V
Sbjct: 181 EYWSSKSYGYYNTSLSLPDDAKVEDIKAELKNGVLNLVIPRTEKPKKNVQEISV 231

[Close]

BLAST of HG10002222 vs. ExPASy Swiss-Prot
Match: B7EZJ7 (23.6 kDa heat shock protein, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=HSP23.6 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.8e-17
Identity = 63/171 (36.84%), Postives = 101/171 (59.06%), Query Frame = 0


Query: 42  EGNPKEVAVNNEDRNTKIVPKREGKKWVPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLS 101
           +G+ +EVAV   DR+ +  P R+ + +VP R   G   +G++L Q AE L R L      
Sbjct: 65  DGHSREVAV--VDRSRRRWPWRDLRDFVPLRLVDG---IGSALSQVAETLTRPLT----- 124

Query: 102 RPWWVSGGHVKEQEDWYKLRVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEE-----EE 161
                  G V+E E+ Y+LR E+PG+ K++V+V V+  +L+I GE R+  EE+     + 
Sbjct: 125 -------GKVREDEERYRLRFEVPGLGKDDVRVYVDDGVLAIHGEKRDVVEEDRGRDGDG 184

Query: 162 FAESYGYYESTVMLPEDAVADEIKAELKDGVLTITIPRTEKPPKDVKEITV 208
              +   Y + ++LPEDAVA+ I AE++DGVL +T+PR+ +  + V E+ V
Sbjct: 185 ECWAAATYHAGLLLPEDAVAEGITAEVRDGVLHVTVPRSPERKRSVTEVKV 218

[Close]

BLAST of HG10002222 vs. ExPASy Swiss-Prot
Match: Q95661 (Small heat shock protein, chloroplastic OS=Solanum lycopersicum OX=4081 GN=HSP21 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 3.8e-15
Identity = 45/111 (40.54%), Postives = 74/111 (66.67%), Query Frame = 0


Query: 92  NRVLKSLNLSRPWWVSGGHVKEQEDWYKLRVEMPGIAKEEVKVTVEGRILSIRGEHREEE 151
           NR   +  +  PW      + + E+  K+R +MPG++KE+VKV+VE  +L I+GEH++EE
Sbjct: 123 NRASGTGEIRTPW-----DIHDDENEIKMRFDMPGLSKEDVKVSVENDMLVIKGEHKKEE 182

Query: 152 E--EEEEFAESYGYYESTVMLPEDAVADEIKAELKDGVLTITIPRTEKPPK 201
           +  ++  +  +Y  Y++ + LP++ V D+IKAELK+GVL I+IP+TE   K
Sbjct: 183 DGRDKHSWGRNYSSYDTRLSLPDNVVKDKIKAELKNGVLFISIPKTEVEKK 228

[Close]

BLAST of HG10002222 vs. ExPASy Swiss-Prot
Match: P30222 (Small heat shock protein, chloroplastic OS=Petunia hybrida OX=4102 GN=HSP22 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 8.6e-15
Identity = 45/104 (43.27%), Postives = 73/104 (70.19%), Query Frame = 0


Query: 103 PWWVSGGHVKEQEDWYKLRVEMPGIAKEEVKVTVEGRILSIRGEHREEEE-EEEEFAESY 162
           PW      +K+ E+  K+R +MPG++KEEVKV+VE  +L I+GEH++EE  +++ +  +Y
Sbjct: 141 PW-----DIKDDENEIKMRFDMPGLSKEEVKVSVEDDVLVIKGEHKKEESGKDDSWGRNY 200

Query: 163 GYYESTVMLPEDAVADEIKAELKDGVLTITIPRT--EKPPKDVK 204
             Y++ + LP++   D++KAELK+GVL I+IP+T  EK   DV+
Sbjct: 201 SSYDTRLSLPDNVDKDKVKAELKNGVLLISIPKTKVEKKVTDVE 239

[Close]

BLAST of HG10002222 vs. ExPASy Swiss-Prot
Match: P09886 (Small heat shock protein, chloroplastic OS=Pisum sativum OX=3888 GN=HSP21 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 5.6e-14
Identity = 62/202 (30.69%), Postives = 105/202 (51.98%), Query Frame = 0


Query: 16  LVSSPADALLPRQTESLIRRLC--EQSSEGNPKEV-AVNNEDRNTKI--VPKREG----- 75
           + S P    LPR     +R     +  ++ N  EV  VN +D+ T +   P+R       
Sbjct: 30  MASFPLRRQLPRLGLRNVRAQAGGDGDNKDNSVEVHRVNKDDQGTAVERKPRRSSIDISP 89

Query: 76  ----KKWVPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGG------HVKEQE 135
                 W P R          S+ Q  + ++R+ +         + GG       +K++E
Sbjct: 90  FGLLDPWSPMR----------SMRQMLDTMDRIFEDAITIPGRNIGGGEIRVPWEIKDEE 149

Query: 136 DWYKLRVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEEFA-ESYGYYESTVMLPEDA 195
              ++R +MPG++KE+VKV+VE  +L I+ +HREE   E+ ++ +SY  Y++ + LP++ 
Sbjct: 150 HEIRMRFDMPGVSKEDVKVSVEDDVLVIKSDHREENGGEDCWSRKSYSCYDTRLKLPDNC 209

Query: 196 VADEIKAELKDGVLTITIPRTE 197
             +++KAELKDGVL ITIP+T+
Sbjct: 210 EKEKVKAELKDGVLYITIPKTK 221

[Close]

BLAST of HG10002222 vs. ExPASy TrEMBL
Match: A0A5A7TMA9 (26.5 kDa heat shock protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001750 PE=3 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 1.8e-95
Identity = 182/210 (86.67%), Postives = 197/210 (93.81%), Query Frame = 0


Query: 1   MALTRLALKNLQQR-ALVSSPADALLPRQTESLIRRLCEQSSEGNPKEVAVNNEDRNTKI 60
           MALTRLAL+NLQQR A VSSP D LLP+QT +LIRR C+QSSEGN KE+AV NE++NTKI
Sbjct: 1   MALTRLALRNLQQRAAFVSSPVDTLLPKQTAALIRRFCDQSSEGNSKEIAVKNEEKNTKI 60

Query: 61  VPKREGKKWVPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGGHVKEQEDWYK 120
            PKREGKKW+PWRKN GEFHLGNSLMQAAE++NRVLKSLNLSRPWW SGGHVKEQEDWYK
Sbjct: 61  APKREGKKWIPWRKNEGEFHLGNSLMQAAEHINRVLKSLNLSRPWWFSGGHVKEQEDWYK 120

Query: 121 LRVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEE--FAESYGYYESTVMLPEDAVAD 180
           LR+EMPGIAK+EVKVTVEGR+L+IRGEHREEEEEEEE  FAESYGYYESTVMLPEDAVA+
Sbjct: 121 LRMEMPGIAKDEVKVTVEGRMLNIRGEHREEEEEEEEEGFAESYGYYESTVMLPEDAVAE 180

Query: 181 EIKAELKDGVLTITIPRTEKPPKDVKEITV 208
           EIKAELKDGVLTITIPRTEKPPKDVKEITV
Sbjct: 181 EIKAELKDGVLTITIPRTEKPPKDVKEITV 210

[Close]

BLAST of HG10002222 vs. ExPASy TrEMBL
Match: A0A1S3BYA0 (26.5 kDa heat shock protein, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103494889 PE=3 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 1.8e-95
Identity = 182/210 (86.67%), Postives = 197/210 (93.81%), Query Frame = 0


Query: 1   MALTRLALKNLQQR-ALVSSPADALLPRQTESLIRRLCEQSSEGNPKEVAVNNEDRNTKI 60
           MALTRLAL+NLQQR A VSSP D LLP+QT +LIRR C+QSSEGN KE+AV NE++NTKI
Sbjct: 1   MALTRLALRNLQQRAAFVSSPVDTLLPKQTAALIRRFCDQSSEGNSKEIAVKNEEKNTKI 60

Query: 61  VPKREGKKWVPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGGHVKEQEDWYK 120
            PKREGKKW+PWRKN GEFHLGNSLMQAAE++NRVLKSLNLSRPWW SGGHVKEQEDWYK
Sbjct: 61  APKREGKKWIPWRKNEGEFHLGNSLMQAAEHINRVLKSLNLSRPWWFSGGHVKEQEDWYK 120

Query: 121 LRVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEE--FAESYGYYESTVMLPEDAVAD 180
           LR+EMPGIAK+EVKVTVEGR+L+IRGEHREEEEEEEE  FAESYGYYESTVMLPEDAVA+
Sbjct: 121 LRMEMPGIAKDEVKVTVEGRMLNIRGEHREEEEEEEEEGFAESYGYYESTVMLPEDAVAE 180

Query: 181 EIKAELKDGVLTITIPRTEKPPKDVKEITV 208
           EIKAELKDGVLTITIPRTEKPPKDVKEITV
Sbjct: 181 EIKAELKDGVLTITIPRTEKPPKDVKEITV 210

[Close]

BLAST of HG10002222 vs. ExPASy TrEMBL
Match: A0A0A0KYF8 (SHSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G005000 PE=3 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 5.3e-92
Identity = 181/210 (86.19%), Postives = 194/210 (92.38%), Query Frame = 0


Query: 1   MALTRLALKNLQQR-ALVSSPADALLPRQTESLIRRLCEQSSEGNPKEVAVNNEDRNTKI 60
           MALTRLAL+NLQQR A  SSP D L P+QT +LIRRL +QSSEGN KE+AV NE+ NTKI
Sbjct: 1   MALTRLALRNLQQRAAFASSPVDTLFPKQTAALIRRLSDQSSEGNSKEIAVKNEE-NTKI 60

Query: 61  VPKREGKKWVPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGGHVKEQEDWYK 120
            PKREGKKWVPWRKN GEFHLGNSLMQAAE++NRVLKSLNLSRPWWVSGG VKEQEDWYK
Sbjct: 61  APKREGKKWVPWRKNEGEFHLGNSLMQAAEHINRVLKSLNLSRPWWVSGGRVKEQEDWYK 120

Query: 121 LRVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEE--FAESYGYYESTVMLPEDAVAD 180
           LRVEMPGIAK+EVKVTVEGR+L+IRGEHREEEEEEE+  FAESYGYYESTVMLPEDAVA+
Sbjct: 121 LRVEMPGIAKDEVKVTVEGRMLNIRGEHREEEEEEEDEGFAESYGYYESTVMLPEDAVAE 180

Query: 181 EIKAELKDGVLTITIPRTEKPPKDVKEITV 208
           EIKAELKDGVLTITIPRTEKPPKDVKEITV
Sbjct: 181 EIKAELKDGVLTITIPRTEKPPKDVKEITV 209

[Close]

BLAST of HG10002222 vs. ExPASy TrEMBL
Match: A0A6J1IQQ6 (26.5 kDa heat shock protein, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111479146 PE=3 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.4e-91
Identity = 177/209 (84.69%), Postives = 190/209 (90.91%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADALLPRQTESLIRRLCEQSSEGNPKEVAVNNEDRNTKIV 60
           MALTRLALKNLQQRALVSSPAD+LL R+TE+LIRR C+QSSEGNPKEVA+NNED+NTK+V
Sbjct: 1   MALTRLALKNLQQRALVSSPADSLLHRRTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 60

Query: 61  PKREGKKWVPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGGHVKEQEDWYKL 120
           PK EGKKWV W+KNG EF +GNSLMQAAEN+NRVLKSL L RPWWVS GHVKEQE+WYKL
Sbjct: 61  PKGEGKKWVLWKKNGREFDVGNSLMQAAENINRVLKSLKLRRPWWVSSGHVKEQEEWYKL 120

Query: 121 RVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEE--FAESYGYYESTVMLPEDAVADE 180
           RVEMPGI KE+VKV VEGRILSIRGEH EE+EEEE     E YGYYESTVMLPEDAVADE
Sbjct: 121 RVEMPGIGKEDVKVRVEGRILSIRGEHSEEDEEEESGWSGERYGYYESTVMLPEDAVADE 180

Query: 181 IKAELKDGVLTITIPRTEKPPKDVKEITV 208
           IKAELKDGVLTITIPRTE PPKDVKEITV
Sbjct: 181 IKAELKDGVLTITIPRTEMPPKDVKEITV 209

[Close]

BLAST of HG10002222 vs. ExPASy TrEMBL
Match: A0A6J1GVM4 (26.5 kDa heat shock protein, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457576 PE=3 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 4.5e-91
Identity = 178/209 (85.17%), Postives = 190/209 (90.91%), Query Frame = 0


Query: 1   MALTRLALKNLQQRALVSSPADALLPRQTESLIRRLCEQSSEGNPKEVAVNNEDRNTKIV 60
           MALTRLALKNLQQRALVSS ADALL RQTE+LIRR C+QSSEGNPKEVA+NNED+NTK+V
Sbjct: 80  MALTRLALKNLQQRALVSSSADALLHRQTEALIRRFCDQSSEGNPKEVAINNEDKNTKMV 139

Query: 61  PKREGKKWVPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGGHVKEQEDWYKL 120
           PK EGK+WV W+KNG EF +GNSLMQAAEN+NRVLKSLNL RPWWVSGGHVKEQE+WYKL
Sbjct: 140 PKGEGKRWVLWKKNGREFDVGNSLMQAAENINRVLKSLNLRRPWWVSGGHVKEQEEWYKL 199

Query: 121 RVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEE--FAESYGYYESTVMLPEDAVADE 180
           RVEMPGI KE+VKV VEGRILSIRGEH EE+EEEE       YGYYESTVMLPEDAVADE
Sbjct: 200 RVEMPGIRKEDVKVRVEGRILSIRGEHSEEDEEEESGWRGGRYGYYESTVMLPEDAVADE 259

Query: 181 IKAELKDGVLTITIPRTEKPPKDVKEITV 208
           IKAELKDGVLTITIPRTE PPKDVKEITV
Sbjct: 260 IKAELKDGVLTITIPRTEMPPKDVKEITV 288

[Close]

BLAST of HG10002222 vs. TAIR 10
Match: AT1G52560.2 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 146.4 bits (368), Expect = 2.6e-35
Identity = 94/227 (41.41%), Postives = 144/227 (63.44%), Query Frame = 0


Query: 1   MALTRLALKNLQQR---ALVSSPADALL------PRQTESLIRRLCEQSSEGNPKEVAVN 60
           MAL RLAL+NLQQ+   +L+    +  L      P +    +     +  +    EV+V+
Sbjct: 1   MALARLALRNLQQKLSPSLMGQSCERGLVGNRHNPMKLNRFMATSAGEQEDKMNTEVSVS 60

Query: 61  NEDRNTKIVPKREGKKWVPWRKNGGEFHL-----GNSLMQAAENLNRVLKSLNLSRPWWV 120
            +    +  P+R G+K + WR      +      GN+L+QA EN+NR+  + N++ P+ +
Sbjct: 61  EKKSPRQNFPRRRGRKSL-WRNTDDHGYFTPTLNGNTLIQATENMNRIFDNFNVN-PFQL 120

Query: 121 SGGHVKEQEDWYKLRVEMPGIAKEEVKVTVEGRILSIRGEHREEEE----EEEEF--AES 180
             G VKEQ+D YKLR E+PG+ KE+VK+TV   IL+I+G+H+ EEE    EE+E+  ++S
Sbjct: 121 M-GQVKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKS 180

Query: 181 YGYYESTVMLPEDAVADEIKAELKDGVLTITIPRTEKPPKDVKEITV 208
           YGYY +++ LP+DA  ++IKAELK+GVL + IPRTEKP K+V+EI+V
Sbjct: 181 YGYYNTSLSLPDDAKVEDIKAELKNGVLNLVIPRTEKPKKNVQEISV 224

[Close]

BLAST of HG10002222 vs. TAIR 10
Match: AT1G52560.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 144.8 bits (364), Expect = 7.6e-35
Identity = 96/234 (41.03%), Postives = 146/234 (62.39%), Query Frame = 0


Query: 1   MALTRLALKNLQQR---ALVSSPADALL------PRQTESLIRRLCEQSSEGNPKEVAVN 60
           MAL RLAL+NLQQ+   +L+    +  L      P +    +     +  +    EV+V+
Sbjct: 1   MALARLALRNLQQKLSPSLMGQSCERGLVGNRHNPMKLNRFMATSAGEQEDKMNTEVSVS 60

Query: 61  NEDRNTKIVPKREGKKWVPWRKNG---------GEFH---LGNSLMQAAENLNRVLKSLN 120
            +    +  P+R G+K + WR             EF    +GN+L+QA EN+NR+  + N
Sbjct: 61  EKKSPRQNFPRRRGRKSL-WRNTDDHGYFTPTLNEFFPPTIGNTLIQATENMNRIFDNFN 120

Query: 121 LSRPWWVSGGHVKEQEDWYKLRVEMPGIAKEEVKVTVEGRILSIRGEHREEEE----EEE 180
           ++ P+ +  G VKEQ+D YKLR E+PG+ KE+VK+TV   IL+I+G+H+ EEE    EE+
Sbjct: 121 VN-PFQLM-GQVKEQDDCYKLRYEVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEED 180

Query: 181 EF--AESYGYYESTVMLPEDAVADEIKAELKDGVLTITIPRTEKPPKDVKEITV 208
           E+  ++SYGYY +++ LP+DA  ++IKAELK+GVL + IPRTEKP K+V+EI+V
Sbjct: 181 EYWSSKSYGYYNTSLSLPDDAKVEDIKAELKNGVLNLVIPRTEKPKKNVQEISV 231

[Close]

BLAST of HG10002222 vs. TAIR 10
Match: AT4G27670.1 (heat shock protein 21 )

HSP 1 Score: 78.6 bits (192), Expect = 6.7e-15
Identity = 40/94 (42.55%), Postives = 64/94 (68.09%), Query Frame = 0


Query: 103 PWWVSGGHVKEQEDWYKLRVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEEFAESYG 162
           PW      +KE+E   K+R +MPG++KE+VK++VE  +L I+GE ++E+ ++     S  
Sbjct: 128 PW-----DIKEEEHEIKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVS 187

Query: 163 YYESTVMLPEDAVADEIKAELKDGVLTITIPRTE 197
            Y + + LP++   D+IKAELK+GVL ITIP+T+
Sbjct: 188 SYGTRLQLPDNCEKDKIKAELKNGVLFITIPKTK 216

[Close]

BLAST of HG10002222 vs. TAIR 10
Match: AT4G25200.1 (mitochondrion-localized small heat shock protein 23.6 )

HSP 1 Score: 65.9 bits (159), Expect = 4.5e-11
Identity = 63/212 (29.72%), Postives = 103/212 (48.58%), Query Frame = 0


Query: 6   LALKNLQQRALVSSPADALLPRQTESLIRRLCEQSSEGNPKEVAVNNEDRNTKIVPKREG 65
           LALK L   ++       L P  +  L      +S + + +    +  D   + VP+R G
Sbjct: 5   LALKRLLSSSIAPRSRSVLRPAVSSRLFNTNAVRSYDDDGEN--GDGVDLYRRSVPRRRG 64

Query: 66  ----KKWVPWRKNGGEFHLGNSLMQAAENLNRVLKSLNLSRPWWVSGGH----VKEQEDW 125
                 + P+        + N + Q  EN       L+ +R    SG      +KE++D 
Sbjct: 65  DFFSDVFDPFSPTRSVSQVLNLMDQFMEN-----PLLSATRGMGASGARRGWDIKEKDDA 124

Query: 126 YKLRVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEEFAESYGYYESTVMLPEDAV-A 185
             LR++MPG+++E+VK+ +E   L IRGE + EE+  EE       + S + LP+     
Sbjct: 125 LYLRIDMPGLSREDVKLALEQDTLVIRGEGKNEEDGGEEGESGNRRFTSRIGLPDKIYKI 184

Query: 186 DEIKAELKDGVLTITIPR-TEKPPKDVKEITV 208
           DEIKAE+K+GVL + IP+  E+   DV++I +
Sbjct: 185 DEIKAEMKNGVLKVVIPKMKEQERNDVRQIEI 209

[Close]

BLAST of HG10002222 vs. TAIR 10
Match: AT5G51440.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.0e-10
Identity = 41/102 (40.20%), Postives = 66/102 (64.71%), Query Frame = 0


Query: 108 GGHVKEQEDWYKLRVEMPGIAKEEVKVTVEGRILSIRGEHREEEEEEEEFAESYGYYEST 167
           G +VKE++D   LR++MPG+++E+VK+ +E   L IRGE   E EE E+ +     + S 
Sbjct: 110 GWNVKEKDDALHLRIDMPGLSREDVKLALEQNTLVIRGE--GETEEGEDVSGDGRRFTSR 169

Query: 168 VMLPEDAV-ADEIKAELKDGVLTITIPRTEKPPK-DVKEITV 208
           + LPE     DEIKAE+K+GVL + IP+ ++  + +++ I V
Sbjct: 170 IELPEKVYKTDEIKAEMKNGVLKVVIPKIKEDERNNIRHINV 209

The following BLAST results are available for this feature:

BLAST of HG10002222 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899919.1	3.2e-99	92.31	26.5 kDa heat shock protein, mitochondrial [Benincasa hispida]	[more]
XP_008454491.1	3.6e-95	86.67	PREDICTED: 26.5 kDa heat shock protein, mitochondrial [Cucumis melo] >KAA0044442...	[more]
XP_004152349.1	1.1e-91	86.19	26.5 kDa heat shock protein, mitochondrial [Cucumis sativus] >KGN52891.1 hypothe...	[more]
KAG6581923.1	7.1e-91	85.17	26.5 kDa heat shock protein, mitochondrial, partial [Cucurbita argyrosperma subs...	[more]
XP_022979416.1	7.1e-91	84.69	26.5 kDa heat shock protein, mitochondrial [Cucurbita maxima]	[more]

BLAST of HG10002222 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SSQ8	1.1e-33	41.03	26.5 kDa heat shock protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HS...	[more]
B7EZJ7	1.8e-17	36.84	23.6 kDa heat shock protein, mitochondrial OS=Oryza sativa subsp. japonica OX=39...	[more]
Q95661	3.8e-15	40.54	Small heat shock protein, chloroplastic OS=Solanum lycopersicum OX=4081 GN=HSP21...	[more]
P30222	8.6e-15	43.27	Small heat shock protein, chloroplastic OS=Petunia hybrida OX=4102 GN=HSP22 PE=2...	[more]
P09886	5.6e-14	30.69	Small heat shock protein, chloroplastic OS=Pisum sativum OX=3888 GN=HSP21 PE=2 S...	[more]

BLAST of HG10002222 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TMA9	1.8e-95	86.67	26.5 kDa heat shock protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BYA0	1.8e-95	86.67	26.5 kDa heat shock protein, mitochondrial OS=Cucumis melo OX=3656 GN=LOC1034948...	[more]
A0A0A0KYF8	5.3e-92	86.19	SHSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G005000 PE=3 S...	[more]
A0A6J1IQQ6	3.4e-91	84.69	26.5 kDa heat shock protein, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1GVM4	4.5e-91	85.17	26.5 kDa heat shock protein, mitochondrial isoform X1 OS=Cucurbita moschata OX=3...	[more]

BLAST of HG10002222 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52560.2	2.6e-35	41.41	HSP20-like chaperones superfamily protein	[more]
AT1G52560.1	7.6e-35	41.03	HSP20-like chaperones superfamily protein	[more]
AT4G27670.1	6.7e-15	42.55	heat shock protein 21	[more]
AT4G25200.1	4.5e-11	29.72	mitochondrion-localized small heat shock protein 23.6	[more]
AT5G51440.1	1.0e-10	40.20	HSP20-like chaperones superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 82..208 e-value: 2.2E-29 score: 103.8
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 98..206
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 111..207 e-value: 8.2E-25 score: 86.9
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 95..208 score: 25.101711
IPR031107	Small heat shock protein HSP20	PANTHER	PTHR11527	HEAT-SHOCK PROTEIN 20 FAMILY MEMBER	coord: 43..207
None	No IPR available	PANTHER	PTHR11527:SF296	26.5 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL	coord: 43..207
None	No IPR available	CDD	cd06464	ACD_sHsps-like	coord: 111..194 e-value: 1.69665E-26 score: 95.3136

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10002222.1	HG10002222.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051259	protein complex oligomerization
biological_process	GO:0006457	protein folding
biological_process	GO:0009408	response to heat
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0009651	response to salt stress
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0051082	unfolded protein binding