HG10001777 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10001777 vs. NCBI nr
Match: XP_008453992.2 (PREDICTED: LOW QUALITY PROTEIN: isoleucine--tRNA ligase, cytoplasmic-like [Cucumis melo])

HSP 1 Score: 1872.1 bits (4848), Expect = 0.0e+00
Identity = 970/1209 (80.23%), Postives = 984/1209 (81.39%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNAND INKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDGINKSNPKKKGSSNSKTE 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            N+VEDSFEILEEVLGASLVGKKYVPLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEA------- 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCFT+KITDFSGRYVKDADKDIIEA       
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  ----------------------------------------------------VKEKRFHN 480
                                                                VKEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK------------- 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVK             
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  -----------------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
                             VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA----- 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA     
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  -----------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
                                   VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL------------------------ 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL                        
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  ---------------------------SLLGKRLWKTMGVVAKEVKAMSQEDILAFEKVG 960
                                       S+LGKRL K MGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            E+TIATHCL+LSEIKVVRDF+RPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EITIATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

BLAST of HG10001777 vs. NCBI nr
Match: KAA0044582.1 (isoleucine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1871.7 bits (4847), Expect = 0.0e+00
Identity = 969/1205 (80.41%), Postives = 984/1205 (81.66%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNAND INKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDGINKSNPKKKGSSNSKTE 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            N+VEDSFEILEEVLGASLVGKKYVPLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEA------- 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCFT+KITDFSGRYVKDADKDIIEA       
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  ------------------------------------------------VKEKRFHNWLEN 480
                                                            VKEKRFHNWLEN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWQKLKENLLENNEKTYWVPDFVKEKRFHNWLEN 480

Query: 481  ARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK----------------- 540
            ARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVK                 
Sbjct: 481  ARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHITIPSR 540

Query: 541  -------------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFY 600
                         VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFY
Sbjct: 541  RGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFY 600

Query: 601  TLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA--------- 660
            TLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA         
Sbjct: 601  TLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLIN 660

Query: 661  -------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKS 720
                               VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKS
Sbjct: 661  SPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKS 720

Query: 721  FNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGE 780
            FNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGE
Sbjct: 721  FNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGE 780

Query: 781  EDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEGGKRG 840
            EDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEGGKRG
Sbjct: 781  EDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEGGKRG 840

Query: 841  ERIEESVTRMMTIIDLARNIRERHNKPLKAPL---------------------------- 900
            ERIEESVTRMMTIIDLARNIRERHNKPLKAPL                            
Sbjct: 841  ERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYVLEEL 900

Query: 901  -----------------------SLLGKRLWKTMGVVAKEVKAMSQEDILAFEKVGEVTI 960
                                   S+LGKRL K MGVVAKEVKAMSQEDILAFEKVGE+TI
Sbjct: 901  NIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVGEITI 960

Query: 961  ATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNRI 1020
            ATHCL+LSEIKVVRDF+RPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNRI
Sbjct: 961  ATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNRI 1020

BLAST of HG10001777 vs. NCBI nr
Match: XP_004152106.1 (isoleucine--tRNA ligase, cytoplasmic [Cucumis sativus] >XP_011653047.1 isoleucine--tRNA ligase, cytoplasmic [Cucumis sativus] >XP_011653048.1 isoleucine--tRNA ligase, cytoplasmic [Cucumis sativus] >XP_031739930.1 isoleucine--tRNA ligase, cytoplasmic [Cucumis sativus] >KGN53060.1 hypothetical protein Csa_014568 [Cucumis sativus])

HSP 1 Score: 1866.3 bits (4833), Expect = 0.0e+00
Identity = 968/1209 (80.07%), Postives = 982/1209 (81.22%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLY+KGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNA NAND INKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            N+VEDSFEILEEVLGASLVGKKYVPLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEA------- 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCF +KITDFSGRYVKDADKDIIEA       
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  ----------------------------------------------------VKEKRFHN 480
                                                                VKEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK------------- 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVK             
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  -----------------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
                             VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA----- 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA     
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  -----------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
                                   VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL------------------------ 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL                        
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  ---------------------------SLLGKRLWKTMGVVAKEVKAMSQEDILAFEKVG 960
                                       S+LGKRL K MGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            EVTIATHCLKLSEIKVVRDF+RPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

BLAST of HG10001777 vs. NCBI nr
Match: XP_038898074.1 (isoleucine--tRNA ligase, cytoplasmic isoform X2 [Benincasa hispida])

HSP 1 Score: 1859.7 bits (4816), Expect = 0.0e+00
Identity = 969/1209 (80.15%), Postives = 979/1209 (80.98%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMV FPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNAND++ KSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDSV-KSNPKKKGSSNSKTE 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            N+VEDSFEILEEVLGASLVGKKYVPLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEA------- 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEA       
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  ----------------------------------------------------VKEKRFHN 480
                                                                VKEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKEKLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK------------- 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVK             
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  -----------------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
                             VFDCWFESGSMPYAYIHYPFENVE FEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSKRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVEHFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA----- 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA     
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  -----------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
                                   VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL------------------------ 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL                        
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHSDKDFLDDIAGKLREYV 900

Query: 901  ---------------------------SLLGKRLWKTMGVVAKEVKAMSQEDILAFEKVG 960
                                       S+LGKRL K MGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLITCDDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            EVTIATHCL+LSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

BLAST of HG10001777 vs. NCBI nr
Match: XP_023537189.1 (isoleucine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1829.3 bits (4737), Expect = 0.0e+00
Identity = 952/1210 (78.68%), Postives = 969/1210 (80.08%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGI+KYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIEKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMV FPVL DP NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLEDPHNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNA-NDAINKSNPKKKGSSNSKT 300
            NANF+YVKVRNKVSGKV+VVAKSRLSALPVEKQKVNAPN   D   KSNPK KGSSNSKT
Sbjct: 241  NANFIYVKVRNKVSGKVFVVAKSRLSALPVEKQKVNAPNGPTDVSKKSNPKTKGSSNSKT 300

Query: 301  ENAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            EN+VEDSFEILEEVLGASLVGKKY PLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSFEILEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEA------ 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEA      
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEAVKAQGR 420

Query: 421  -----------------------------------------------------VKEKRFH 480
                                                                 VKEKRFH
Sbjct: 421  LVKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKEKLLENNEKTYWVPDFVKEKRFH 480

Query: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK------------ 540
            NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSG K            
Sbjct: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGAKVFDLHRHKIDHI 540

Query: 541  ------------------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600
                              VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT
Sbjct: 541  TIPSQRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600

Query: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA---- 660
            RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEI+NDYGA    
Sbjct: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIVNDYGADALR 660

Query: 661  ------------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA 720
                                    VRDVFLPWYNAYRFLVQNAKRLEIEG A FSP+DQA
Sbjct: 661  LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGLAQFSPLDQA 720

Query: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780
            TLQKSFNVLDQWINSATQSLVYFVRKEMD YRLYTVVPYLLKFLDNLTNIYVRFNRKRLK
Sbjct: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDAYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780

Query: 781  GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE 840
            GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE
Sbjct: 781  GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE 840

Query: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL----------------------- 900
            GGKRGERIEESVTRMMTIIDLARNIRERHNKPLK PL                       
Sbjct: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDDIAGKLREY 900

Query: 901  ----------------------------SLLGKRLWKTMGVVAKEVKAMSQEDILAFEKV 960
                                        SLLGKRL K MGVVAKEVKAMSQEDILAFEKV
Sbjct: 901  VLEELNIRSLIPCNDTLKYASLRAEPDFSLLGKRLGKAMGVVAKEVKAMSQEDILAFEKV 960

Query: 961  GEVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVARE 1020
            G VTIATHCL+LSEIKVVRDFRRPDGMTDKEIDA+GDGDVLVILDLRPDESLFEAGVARE
Sbjct: 961  GNVTIATHCLRLSEIKVVRDFRRPDGMTDKEIDASGDGDVLVILDLRPDESLFEAGVARE 1020

BLAST of HG10001777 vs. ExPASy Swiss-Prot
Match: F4JLM5 (Isoleucine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g10320 PE=2 SV=1)

HSP 1 Score: 1464.5 bits (3790), Expect = 0.0e+00
Identity = 767/1221 (62.82%), Postives = 866/1221 (70.93%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            M+EVCEGK+FSFP+ EE VL +W++I AF+TQL+RT +LPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MEEVCEGKEFSFPRQEEDVLSFWTEIDAFKTQLKRTENLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQ MTGHHVTRRFGWDCHGLPVENEID+KL IKRRD+V+KMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQTMTGHHVTRRFGWDCHGLPVENEIDRKLNIKRRDEVIKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYV EWEK+ITR GRWIDFKNDYKTMDL FMESVWWVFSQL+EK LVY+GFKVM
Sbjct: 121  CRSIVTRYVAEWEKVITRCGRWIDFKNDYKTMDLPFMESVWWVFSQLWEKNLVYRGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQ+YK+VPDPEIMV FPV+GD  NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQNYKEVPDPEIMVTFPVIGDQDNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPN--ANDAINKSNPKKKGSSNSK 300
            NA FVYVKVRNK +GKVY+VA+SRLSALP +K K    N  A D   K+NPK KG   +K
Sbjct: 241  NAKFVYVKVRNKNTGKVYIVAESRLSALPTDKPKAKLSNGPAGDT-KKANPKAKG---AK 300

Query: 301  TENAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCA 360
             E+A  DS+E+LE+  GASLVGKKY PLFDYF++FS  AFRVVAD YVTDDSGTGIVHCA
Sbjct: 301  PESAA-DSYEVLEKFNGASLVGKKYEPLFDYFSDFSSEAFRVVADDYVTDDSGTGIVHCA 360

Query: 361  PAFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEA----- 420
            PAFGEDDYRVC+ N++I KGENL+VAVDDDG FT +IT FSGRYVKDADKDIIEA     
Sbjct: 361  PAFGEDDYRVCLLNKIIKKGENLVVAVDDDGLFTERITHFSGRYVKDADKDIIEAVKAKG 420

Query: 421  ------------------------------------------------------VKEKRF 480
                                                                  VK+KRF
Sbjct: 421  RLVKTGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEQLKEKLLKSNEQTEWVPGYVKDKRF 480

Query: 481  HNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK----------- 540
            HNWLENARDWA+SRSRFWGTPLP+W+S+DGEE+++MDS+EKLE+LSGVK           
Sbjct: 481  HNWLENARDWAISRSRFWGTPLPIWISDDGEEVVIMDSVEKLEKLSGVKVFDLHRHHIDH 540

Query: 541  -------------------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQ 600
                               VFDCWFESGSMPYAYIHYPFEN ELFEKNFPG FVAEGLDQ
Sbjct: 541  ITIPSSRGDEFGVLRRVEDVFDCWFESGSMPYAYIHYPFENKELFEKNFPGDFVAEGLDQ 600

Query: 601  TRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA--- 660
            TRGWFYTLMVLSTALF KPAF+NLICNGLVLAEDGKKM+K+L+NYP P+E+I++YGA   
Sbjct: 601  TRGWFYTLMVLSTALFEKPAFKNLICNGLVLAEDGKKMAKKLRNYPPPLEVIDEYGADAV 660

Query: 661  -------------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQ 720
                                     V+DVFLPWYNAYRFLVQNAKRLE EG  PF P D 
Sbjct: 661  RLYLINSPVVRAEPLRFKKEGVLGVVKDVFLPWYNAYRFLVQNAKRLETEGGVPFVPTDL 720

Query: 721  ATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRL 780
            AT+Q S N+LDQWI+SATQSLV FVR+EMD YRLYTVVP LLKFLDNLTNIYVRFNRKRL
Sbjct: 721  ATIQ-SANILDQWIHSATQSLVRFVREEMDAYRLYTVVPRLLKFLDNLTNIYVRFNRKRL 780

Query: 781  KGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQ 840
            KGRTGE+DC  ALSTL++VLLTSCKVMAPFTPFFTE LYQNLRK   GSEES+HYCS P 
Sbjct: 781  KGRTGEDDCHTALSTLFNVLLTSCKVMAPFTPFFTETLYQNLRKACKGSEESVHYCSIPP 840

Query: 841  EGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL---------------------- 900
              G  GERIE SVTRMM IIDLARNIRER+  PLK PL                      
Sbjct: 841  REGMEGERIELSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADFLNDITGVLRE 900

Query: 901  -----------------------------SLLGKRLWKTMGVVAKEVKAMSQEDILAFEK 960
                                         S+LGKRL K+MG+VAKEVK MSQ+DILAFE+
Sbjct: 901  YVLEELNVRSLVPCNDTLKYASLKAEPDFSVLGKRLGKSMGLVAKEVKEMSQKDILAFEE 960

Query: 961  VGEVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAR 1020
             GEVTIA H LK ++IK+VR F+RPD + + EID+AGDGDVLVILDLR D+SL EAG AR
Sbjct: 961  AGEVTIANHLLKETDIKIVRVFKRPDDLKENEIDSAGDGDVLVILDLRADDSLVEAGFAR 1020

BLAST of HG10001777 vs. ExPASy Swiss-Prot
Match: P41252 (Isoleucine--tRNA ligase, cytoplasmic OS=Homo sapiens OX=9606 GN=IARS1 PE=1 SV=2)

HSP 1 Score: 899.4 bits (2323), Expect = 3.7e-260
Identity = 473/1049 (45.09%), Postives = 623/1049 (59.39%), Query Frame = 0

Query: 8    KDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKD 67
            ++ +FP  EE +LE+W++   F+  L++++  P++ FYDGPPFATGLPHYGHILAGTIKD
Sbjct: 7    ENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKD 66

Query: 68   IVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTR 127
            IVTRY   +G HV RRFGWDCHGLPVE EID+ LGI+  +DV KMGI +YN +CR+IV R
Sbjct: 67   IVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMR 126

Query: 128  YVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCK 187
            Y  EW+  ++R GRWIDF NDYKT+  +FMESVWWVF QLY+KGLVY+G KVMP+ST C 
Sbjct: 127  YSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACN 186

Query: 188  TPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCVNANFVYV 247
            TPLSNFE+ Q+YKDV DP + V FP L + +  + VAWTTTPWTLPSNLA+CVN    YV
Sbjct: 187  TPLSNFESHQNYKDVQDPSVFVTFP-LEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYV 246

Query: 248  KVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTENAVEDSF 307
            K+++   G++ ++ ++RLSAL                                  +E  +
Sbjct: 247  KIKDVARGRLLILMEARLSAL--------------------------------YKLESDY 306

Query: 308  EILEEVLGASLVGKKYVPLFDYFNEFSD-VAFRVVADSYVTDDSGTGIVHCAPAFGEDDY 367
            EILE   GA L GKKY PLFDYF +  +  AF V+ D+YV ++ GTG+VH AP FG +DY
Sbjct: 307  EILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDY 366

Query: 368  RVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDII--------------- 427
            RVC++  +I K    +  VD  GCFTT++TDF+G+YVKDADK II               
Sbjct: 367  RVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTF 426

Query: 428  --------------------------------------------EAVKEKRFHNWLENAR 487
                                                        E V+EKRF NWL++AR
Sbjct: 427  THSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLKDAR 486

Query: 488  DWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK------------------- 547
            DW +SR+R+WGTP+P+WVS+D EE++ + S+ +LE+LSG K                   
Sbjct: 487  DWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTIPSRCG 546

Query: 548  ---------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFYTLMV 607
                     VFDCWFESGSMPYA +HYPFEN   FE  FP  F+AEG+DQTRGWFYTL+V
Sbjct: 547  KGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLV 606

Query: 608  LSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA------------- 667
            L+TALFG+P F+N+I NGLVLA DG+KMSKR KNYP P+ II  YGA             
Sbjct: 607  LATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVV 666

Query: 668  ---------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKSFNVL 727
                           ++DV LPWYNAYRFL+QN  RL+ E    F   ++ T+++S N+ 
Sbjct: 667  RAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFL-YNENTVRESPNIT 726

Query: 728  DQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCR 787
            D+WI S  QSL+ F   EM  YRLYTVVP L+KF+D LTN YVR NR+RLKG  G EDC 
Sbjct: 727  DRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCV 786

Query: 788  IALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLR------KVSDGSEESIHYCSYPQEGGK 847
            +AL TL+ VLL+ C++MAP+TPF TE++YQNL+       V D    SIHY   P+   +
Sbjct: 787  MALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREE 846

Query: 848  R-GERIEESVTRMMTIIDLARNIRERHNKPLKAPLS------------------------ 883
               ++ E +V++M ++I+L R IR+R   P+K PL                         
Sbjct: 847  LIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIE 906

BLAST of HG10001777 vs. ExPASy Swiss-Prot
Match: Q8BU30 (Isoleucine--tRNA ligase, cytoplasmic OS=Mus musculus OX=10090 GN=Iars1 PE=1 SV=2)

HSP 1 Score: 896.7 bits (2316), Expect = 2.4e-259
Identity = 478/1047 (45.65%), Postives = 622/1047 (59.41%), Query Frame = 0

Query: 8    KDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKD 67
            ++ SFP  EE +LE+WS    F+  L++++  P++ FYDGPPFATGLPHYGHILAGTIKD
Sbjct: 7    ENISFPAEEEKILEFWSKHNCFQECLKQSKLRPKFTFYDGPPFATGLPHYGHILAGTIKD 66

Query: 68   IVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTR 127
            IVTRY   +G HV RRFGWDCHGLPVE EID+ LGIK  +DV KMGI +YN++CR+IV R
Sbjct: 67   IVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNKQCRAIVMR 126

Query: 128  YVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCK 187
            Y  EW+  +TR GRWIDF NDYKT+  +FMESVWWVF QLY+KGLVY+G KVMP+ST C 
Sbjct: 127  YSAEWKSTVTRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACG 186

Query: 188  TPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCVNANFVYV 247
            TPLSNFE+ Q+YKDV DP + V FP L + +N + VAWTTTPWTLPSNLALCVN    YV
Sbjct: 187  TPLSNFESNQNYKDVQDPSVFVTFP-LEEDENTSLVAWTTTPWTLPSNLALCVNPEIQYV 246

Query: 248  KVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTENAVEDSF 307
            K+++   GK++++ ++RLSAL   KQ                              E  +
Sbjct: 247  KIKDVARGKLFILTEARLSAL--YKQ------------------------------ESDY 306

Query: 308  EILEEVLGASLVGKKYVPLFDYFNEFSD-VAFRVVADSYVTDDSGTGIVHCAPAFGEDDY 367
            EILE   GASL GKKY PLFDYF +  +  AF V+ D YV D+ GTG+VH AP FG DD+
Sbjct: 307  EILERFPGASLKGKKYKPLFDYFIKCKENGAFTVLVDHYVKDEEGTGVVHQAPYFGADDH 366

Query: 368  RVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDII--------------- 427
            RVC++  +I K    +  VD  GCFT ++T F G+YVKDADK+II               
Sbjct: 367  RVCMDFNIIQKDSVPVCPVDASGCFTEEVTHFVGQYVKDADKNIIRMLKEQGRLLAAGTF 426

Query: 428  --------------------------------------------EAVKEKRFHNWLENAR 487
                                                        E V+EKRF NWL+ AR
Sbjct: 427  THSYPFCWRSDTPLIYKSVPSWFVRVEPMVDQLLKNNDLCYWVPEFVREKRFGNWLKEAR 486

Query: 488  DWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK------------------- 547
            DWA+SR+R+WGTP+P+WVSED EE++ + S+ +LE+LSG K                   
Sbjct: 487  DWAISRNRYWGTPIPLWVSEDLEEVVCIGSVAELEELSGTKISDLHRESIDHLTIPSRCG 546

Query: 548  ---------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFYTLMV 607
                     VFDCWFESGSMPYA +HYPFE+   FE  FP  F+AEG+DQTRGWFYTL+V
Sbjct: 547  KAPLRRVSEVFDCWFESGSMPYAQVHYPFESKREFEDAFPADFIAEGIDQTRGWFYTLLV 606

Query: 608  LSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA------------- 667
            L+TALFG+P F+N+I NGL+LA DG+KMSKR KNYP P+ II+ YGA             
Sbjct: 607  LATALFGQPPFKNVIVNGLILASDGQKMSKRKKNYPDPVSIIDKYGADALRLYLINSPVV 666

Query: 668  ---------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKSFNVL 727
                           ++DV LPWYNAYRF +QN  RL  E    F   ++ T+++S N+ 
Sbjct: 667  RAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVFRLHKEEEVKFL-YNEHTVRESPNIT 726

Query: 728  DQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCR 787
            D+W+ S  QSL+ F   EM  YRLYTVVP L+KF+D LTN YVR NR+RLKG +G EDC 
Sbjct: 727  DRWVLSFMQSLLGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGESGVEDCV 786

Query: 788  IALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLR------KVSDGSEESIHYCSYPQEGGK 847
            +AL TL+ VLL+ C++MAP+TPF TE++YQNL+       + D    SIHY   P+   +
Sbjct: 787  MALETLFSVLLSLCRLMAPYTPFLTELMYQNLKLLIDPASLRDKDTLSIHYLMLPRVREE 846

Query: 848  R-GERIEESVTRMMTIIDLARNIRERHNKPLKAPLS------------------------ 881
               ++ E +V+RM ++I+L R IR+R   P+K PL                         
Sbjct: 847  LIDKKTENAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALEDIRSLEKYIIE 906

BLAST of HG10001777 vs. ExPASy Swiss-Prot
Match: Q54YD4 (Probable isoleucine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=ileS PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 8.4e-257
Identity = 472/1059 (44.57%), Postives = 623/1059 (58.83%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            M+EV +  DF+    E  +L+YW DIKAFET ++ +   PEY FYDGPPFATGLPHYGHI
Sbjct: 1    MEEVPQKIDFA--GEESKILKYWDDIKAFETSVKMSEGKPEYSFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKD +TRY   TGHHV RRFGWDCHGLP+E EID+  G++ ++DVLKMGI  YN+ 
Sbjct: 61   LAGTIKDTITRYAHQTGHHVERRFGWDCHGLPIEFEIDKLHGVRTKEDVLKMGIPTYNQH 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIV +Y  EWE ++ R GRWID KN+YKTMD  FMESVWWVF +L++K LVY+GFKVM
Sbjct: 121  CRSIVMKYSHEWEIVVNRMGRWIDMKNNYKTMDTPFMESVWWVFQELFKKDLVYQGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYS GC TPLSNFEA  +YKDV DP  +V+F  L D +  + +AWTTTPWTLPSNLAL V
Sbjct: 181  PYSIGCTTPLSNFEASSNYKDVSDPACVVSFQTL-DDEKVSILAWTTTPWTLPSNLALTV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            N    Y+K+ +    +++++ K+R S L                 KS             
Sbjct: 241  NPKMEYIKINDIKRNQIFILGKNRTSIL----------------YKS------------- 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDV-AFRVVADSYVTDDSGTGIVHCAP 360
               +  + +LE + G  L+GKKYVP+F YF   +++  F V+   +VTDDSGTGIVH AP
Sbjct: 301  ---DKEYTVLETMKGTDLIGKKYVPMFPYFASDANMGGFVVIGGDFVTDDSGTGIVHTAP 360

Query: 361  AFGEDDYRVCIENQVINKGE---NLIVAVDDDGCFTTKITDFSGRYVKDADKD------- 420
            A+GEDD+ VCI N VI++ +    ++ +VD +GCFT+ +TDF+G  VKDA+         
Sbjct: 361  AYGEDDFNVCIANGVISRDQFKRPILNSVDANGCFTSDVTDFAGMMVKDAETTKQISIYL 420

Query: 421  ------------------------------------------------------IIEAVK 480
                                                                  + + VK
Sbjct: 421  KNKGRMVNSANLVHSYPYCWRSDTPLIYKAVGSWFVRVESIRDKLLANNDKTYWVPDFVK 480

Query: 481  EKRFHNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK------- 540
            EKRF NWL+NA DWAVSR+R+WGTP+P+W+SEDGEE++V+ SI++LE+LSGV+       
Sbjct: 481  EKRFANWLKNATDWAVSRNRYWGTPIPLWISEDGEEVVVIGSIDELERLSGVRVTDLHRE 540

Query: 541  ---------------------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGL 600
                                 VFDCWFESGSMPYA  HYPFEN + FEK FP HF+AEGL
Sbjct: 541  SIDHITIPSQKGKGTLRRIEDVFDCWFESGSMPYAQQHYPFENKDKFEKIFPAHFIAEGL 600

Query: 601  DQTRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA- 660
            DQTRGWFYTL+VLSTALF KP F+NLI NGLVLA DGKKMSKRLKNYP PME+++  GA 
Sbjct: 601  DQTRGWFYTLLVLSTALFDKPPFQNLIVNGLVLAADGKKMSKRLKNYPDPMEVVSKVGAD 660

Query: 661  ---------------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPV 720
                                       ++DVFLPW+NAYRF VQN  R E      F P 
Sbjct: 661  SLRLYLINSPVVRAETLKFQEKGVQDMIKDVFLPWFNAYRFFVQNCLRFEKATNTTFQP- 720

Query: 721  DQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRK 780
            D      S NV+D+WI ++ QSL+ FVR EM  YRLYTVVP L++F+++LTN +VR NRK
Sbjct: 721  DIKVALASENVMDKWILASCQSLIAFVRAEMAAYRLYTVVPKLVRFIEDLTNWFVRLNRK 780

Query: 781  RLKGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKV--SDGSEESIHYC 840
            RLKG  G+ DC  AL+ LY VL+T C  M PFTPFFTE +YQNL+K    +   +S+HY 
Sbjct: 781  RLKGSNGDADCLAALNILYEVLMTICIAMGPFTPFFTEYMYQNLKKALPKEKQMDSVHYV 840

Query: 841  SYPQEGGKR-GERIEESVTRMMTIIDLARNIRERHNKPLKAPL----------------- 886
             +P+   +    RIEE+++RM  +I+L R  R+R  KP+K PL                 
Sbjct: 841  MFPEPIQEAFNTRIEEAISRMQVVIELGRAARDRRTKPIKFPLKEFMVITENQQYLADLE 900

BLAST of HG10001777 vs. ExPASy Swiss-Prot
Match: P09436 (Isoleucine--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ILS1 PE=1 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 3.9e-238
Identity = 460/1052 (43.73%), Postives = 591/1052 (56.18%), Query Frame = 0

Query: 10   FSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDIV 69
            FSFPK EE VL  W +I AF T LE T+D PE+ F+DGPPFATG PHYGHILA TIKDIV
Sbjct: 8    FSFPKEEEKVLSLWDEIDAFHTSLELTKDKPEFSFFDGPPFATGTPHYGHILASTIKDIV 67

Query: 70   TRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTRYV 129
             RY  MTGHHV RRFGWD HG+P+E+ ID+KLGI  +DDV K G++ YN ECRSIV  Y 
Sbjct: 68   PRYATMTGHHVERRFGWDTHGVPIEHIIDKKLGITGKDDVFKYGLENYNNECRSIVMTYA 127

Query: 130  GEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCKTP 189
             +W K I R GRWIDF NDYKTM   FMES WW F QL+EKG VY+GFKVMPYSTG  TP
Sbjct: 128  SDWRKTIGRLGRWIDFDNDYKTMYPSFMESTWWAFKQLHEKGQVYRGFKVMPYSTGLTTP 187

Query: 190  LSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCVNANFVYVKV 249
            LSNFEA Q+YKDV DP + + F V+G  +    VAWTTTPWTLPSNL+LCVNA+F YVK+
Sbjct: 188  LSNFEAQQNYKDVNDPAVTIGFNVIGQ-EKTQLVAWTTTPWTLPSNLSLCVNADFEYVKI 247

Query: 250  RNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTENAVEDSFEI 309
             ++   + +++ +S +  L                    PK              + ++I
Sbjct: 248  YDETRDRYFILLESLIKTL-----------------YKKPK-------------NEKYKI 307

Query: 310  LEEVLGASLVGKKYVPLFDYFNE-FSDVAFRVVADSYVTDDSGTGIVHCAPAFGEDDYRV 369
            +E++ G+ LVG KY PLF YF E F + AFRV++D YVT DSGTGIVH APAFGE+D   
Sbjct: 308  VEKIKGSDLVGLKYEPLFPYFAEQFHETAFRVISDDYVTSDSGTGIVHNAPAFGEEDNAA 367

Query: 370  CIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIE---------------- 429
            C++N VI++   L  A+DD G FT  + DF G YVKDADK II+                
Sbjct: 368  CLKNGVISEDSVLPNAIDDLGRFTKDVPDFEGVYVKDADKLIIKYLTNTGNLLLASQIRH 427

Query: 430  -------------------------------------------AVKEKRFHNWLENARDW 489
                                                        +KEKRF NW+ NARDW
Sbjct: 428  SYPFCWRSDTPLLYRSVPAWFVRVKNIVPQMLDSVMKSHWVPNTIKEKRFANWIANARDW 487

Query: 490  AVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK--------------------- 549
             VSR+R+WGTP+P+WVS+D EE++ + SI++LE+L+GV+                     
Sbjct: 488  NVSRNRYWGTPIPLWVSDDFEEVVCVGSIKELEELTGVRNITDLHRDVIDKLTIPSKQGK 547

Query: 550  --------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFYTLMVL 609
                    VFDCWFESGSMPYA  HYPFEN E F++  P +F++EGLDQTRGWFYTL VL
Sbjct: 548  GDLKRIEEVFDCWFESGSMPYASQHYPFENTEKFDERVPANFISEGLDQTRGWFYTLAVL 607

Query: 610  STALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA-------------- 669
             T LFG   ++N+I +G+VLA DG+KMSK LKNYP P  ++N YGA              
Sbjct: 608  GTHLFGSVPYKNVIVSGIVLAADGRKMSKSLKNYPDPSIVLNKYGADALRLYLINSPVLK 667

Query: 670  --------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKSFNVLD 729
                          V  V LPW+N+++FL      L+      F   D     KS NV+D
Sbjct: 668  AESLKFKEEGVKEVVSKVLLPWWNSFKFLDGQIALLKKMSNIDFQYDDSV---KSDNVMD 727

Query: 730  QWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGEEDCRI 789
            +WI ++ QSLV F+ +EM  Y+LYTVVP LL F+D LTN Y+RFNR+RLKG  G EDC  
Sbjct: 728  RWILASMQSLVQFIHEEMGQYKLYTVVPKLLNFIDELTNWYIRFNRRRLKGENGVEDCLK 787

Query: 790  ALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKV----------SDGSEESIHYCSYPQE 849
            AL++L+  L T  + MAPFTPF +E +Y  L++            DG   S+H+ SYP  
Sbjct: 788  ALNSLFDALFTFVRAMAPFTPFLSESIYLRLKEYIPEAVLAKYGKDG--RSVHFLSYPVV 847

Query: 850  GGKR-GERIEESVTRMMTIIDLARNIRERHNKPLKAPLS--------------------- 883
              +   E IE +V+RM ++IDL RNIRE+    LK PL                      
Sbjct: 848  KKEYFDEAIETAVSRMQSVIDLGRNIREKKTISLKTPLKTLVILHSDESYLKDVEALKNY 907

BLAST of HG10001777 vs. ExPASy TrEMBL
Match: A0A1S3BX29 (Isoleucyl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103494549 PE=3 SV=1)

HSP 1 Score: 1872.1 bits (4848), Expect = 0.0e+00
Identity = 970/1209 (80.23%), Postives = 984/1209 (81.39%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNAND INKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDGINKSNPKKKGSSNSKTE 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            N+VEDSFEILEEVLGASLVGKKYVPLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEA------- 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCFT+KITDFSGRYVKDADKDIIEA       
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  ----------------------------------------------------VKEKRFHN 480
                                                                VKEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK------------- 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVK             
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  -----------------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
                             VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA----- 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA     
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  -----------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
                                   VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL------------------------ 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL                        
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  ---------------------------SLLGKRLWKTMGVVAKEVKAMSQEDILAFEKVG 960
                                       S+LGKRL K MGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            E+TIATHCL+LSEIKVVRDF+RPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EITIATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

BLAST of HG10001777 vs. ExPASy TrEMBL
Match: A0A5A7TMQ4 (Isoleucyl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003320 PE=3 SV=1)

HSP 1 Score: 1871.7 bits (4847), Expect = 0.0e+00
Identity = 969/1205 (80.41%), Postives = 984/1205 (81.66%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNAND INKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDGINKSNPKKKGSSNSKTE 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            N+VEDSFEILEEVLGASLVGKKYVPLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEA------- 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCFT+KITDFSGRYVKDADKDIIEA       
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTSKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  ------------------------------------------------VKEKRFHNWLEN 480
                                                            VKEKRFHNWLEN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWQKLKENLLENNEKTYWVPDFVKEKRFHNWLEN 480

Query: 481  ARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK----------------- 540
            ARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVK                 
Sbjct: 481  ARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHITIPSR 540

Query: 541  -------------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFY 600
                         VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFY
Sbjct: 541  RGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFY 600

Query: 601  TLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA--------- 660
            TLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA         
Sbjct: 601  TLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRLYLIN 660

Query: 661  -------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKS 720
                               VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKS
Sbjct: 661  SPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQATLQKS 720

Query: 721  FNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGE 780
            FNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGE
Sbjct: 721  FNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKGRTGE 780

Query: 781  EDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEGGKRG 840
            EDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEGGKRG
Sbjct: 781  EDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEGGKRG 840

Query: 841  ERIEESVTRMMTIIDLARNIRERHNKPLKAPL---------------------------- 900
            ERIEESVTRMMTIIDLARNIRERHNKPLKAPL                            
Sbjct: 841  ERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYVLEEL 900

Query: 901  -----------------------SLLGKRLWKTMGVVAKEVKAMSQEDILAFEKVGEVTI 960
                                   S+LGKRL K MGVVAKEVKAMSQEDILAFEKVGE+TI
Sbjct: 901  NIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVGEITI 960

Query: 961  ATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNRI 1020
            ATHCL+LSEIKVVRDF+RPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNRI
Sbjct: 961  ATHCLRLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREIVNRI 1020

BLAST of HG10001777 vs. ExPASy TrEMBL
Match: A0A0A0KTX5 (Isoleucyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G013540 PE=3 SV=1)

HSP 1 Score: 1866.3 bits (4833), Expect = 0.0e+00
Identity = 968/1209 (80.07%), Postives = 982/1209 (81.22%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLY+KGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYQKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTE 300
            NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNA NAND INKSNPKKKGSSNSKTE
Sbjct: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAANANDGINKSNPKKKGSSNSKTE 300

Query: 301  NAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360
            N+VEDSFEILEEVLGASLVGKKYVPLFDYF EFSDVAFRVVADSYVTDDSGTGIVHCAPA
Sbjct: 301  NSVEDSFEILEEVLGASLVGKKYVPLFDYFKEFSDVAFRVVADSYVTDDSGTGIVHCAPA 360

Query: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEA------- 420
            FGEDDYRVCIENQVINKGENLIVAVDDDGCF +KITDFSGRYVKDADKDIIEA       
Sbjct: 361  FGEDDYRVCIENQVINKGENLIVAVDDDGCFISKITDFSGRYVKDADKDIIEAVKAQGRL 420

Query: 421  ----------------------------------------------------VKEKRFHN 480
                                                                VKEKRFHN
Sbjct: 421  VKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKENLLENNEKTYWVPDFVKEKRFHN 480

Query: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK------------- 540
            WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLE+LSGVK             
Sbjct: 481  WLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEKLSGVKVFDLHRHKIDHIT 540

Query: 541  -----------------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600
                             VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR
Sbjct: 541  IPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTR 600

Query: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA----- 660
            GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA     
Sbjct: 601  GWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALRL 660

Query: 661  -----------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720
                                   VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT
Sbjct: 661  YLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQAT 720

Query: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780
            LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG
Sbjct: 721  LQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLKG 780

Query: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840
            RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG
Sbjct: 781  RTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEG 840

Query: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL------------------------ 900
            GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL                        
Sbjct: 841  GKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPLREMIVVHPDKDFLDDIAGKLREYV 900

Query: 901  ---------------------------SLLGKRLWKTMGVVAKEVKAMSQEDILAFEKVG 960
                                       S+LGKRL K MGVVAKEVKAMSQEDILAFEKVG
Sbjct: 901  LEELNIRSLIPCNDTLKYASLRAEPDFSMLGKRLGKAMGVVAKEVKAMSQEDILAFEKVG 960

Query: 961  EVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020
            EVTIATHCLKLSEIKVVRDF+RPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI
Sbjct: 961  EVTIATHCLKLSEIKVVRDFKRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAREI 1020

BLAST of HG10001777 vs. ExPASy TrEMBL
Match: A0A6J1GTY1 (Isoleucyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457524 PE=3 SV=1)

HSP 1 Score: 1825.1 bits (4726), Expect = 0.0e+00
Identity = 947/1210 (78.26%), Postives = 973/1210 (80.41%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEE VLEYWSDI+AFETQLERT+DLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEVVLEYWSDIRAFETQLERTKDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQL+EKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLFEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMV FPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPN-ANDAINKSNPKKKGSSNSKT 300
            NANFVYVKVRNK SGKVYVVAKSRLSALPVEKQKVNAPN + + +NKSNPKKKGSSNSKT
Sbjct: 241  NANFVYVKVRNKASGKVYVVAKSRLSALPVEKQKVNAPNGSTEVVNKSNPKKKGSSNSKT 300

Query: 301  ENAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            EN+VEDS+E+LEEVLGASLVGKKY PLFDYF EFSDVAFRVVAD+YVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSYEVLEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADNYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEA------ 420
            AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKIT FSGRYVKDADKDIIEA      
Sbjct: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITQFSGRYVKDADKDIIEAVKAQGR 420

Query: 421  -----------------------------------------------------VKEKRFH 480
                                                                 VKEKRFH
Sbjct: 421  LVKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKEKLLENNEKTYWVPDFVKEKRFH 480

Query: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK------------ 540
            NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK            
Sbjct: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHI 540

Query: 541  ------------------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600
                              VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT
Sbjct: 541  TIPSRRGPEYGVLRRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600

Query: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA---- 660
            RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA    
Sbjct: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR 660

Query: 661  ------------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA 720
                                    VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA
Sbjct: 661  LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA 720

Query: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780
            TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK
Sbjct: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780

Query: 781  GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE 840
            GRTGEEDCRIALSTLYHVLLTSC VMAPFTPFFTE LYQNLRKVSDGSEESIHYCSYPQE
Sbjct: 781  GRTGEEDCRIALSTLYHVLLTSCMVMAPFTPFFTEALYQNLRKVSDGSEESIHYCSYPQE 840

Query: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL----------------------- 900
            GGKRGERIEESVTRMMTIIDLARNIRERHNKPLK PL                       
Sbjct: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDDIAGKLREY 900

Query: 901  ----------------------------SLLGKRLWKTMGVVAKEVKAMSQEDILAFEKV 960
                                        S+LGKRL K MGVVAKEVKAMSQEDILAFEKV
Sbjct: 901  VLEELNIRSLIPCNDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKV 960

Query: 961  GEVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVARE 1020
            GEVTIATHCL+LS+IKVVRDF+RPDGMTDKE+DAAGDGDVLVILDLRPDESLFEAGVARE
Sbjct: 961  GEVTIATHCLRLSDIKVVRDFKRPDGMTDKEMDAAGDGDVLVILDLRPDESLFEAGVARE 1020

BLAST of HG10001777 vs. ExPASy TrEMBL
Match: A0A6J1IPC3 (Isoleucyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479322 PE=3 SV=1)

HSP 1 Score: 1824.7 bits (4725), Expect = 0.0e+00
Identity = 947/1210 (78.26%), Postives = 973/1210 (80.41%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            MDEVCEGKDFSFPKHEE VLEYWSDI+AFETQLERT+DLPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MDEVCEGKDFSFPKHEEVVLEYWSDIRAFETQLERTKDLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQL+EKGLVYKGFKVM
Sbjct: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLFEKGLVYKGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQSYKDVPDPEIMV FPVLGDPQNAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVTFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPN-ANDAINKSNPKKKGSSNSKT 300
            NANFVYVKVRNK SGKVYVVAKSRLSALPVEKQKVNAPN + D +NKSNPKKKGSSNSKT
Sbjct: 241  NANFVYVKVRNKASGKVYVVAKSRLSALPVEKQKVNAPNGSTDVVNKSNPKKKGSSNSKT 300

Query: 301  ENAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAP 360
            EN+VEDS+E+LEEVLGASLVGKKY PLFDYF EFSDVAFRVVAD+YVTDDSGTGIVHCAP
Sbjct: 301  ENSVEDSYEVLEEVLGASLVGKKYEPLFDYFKEFSDVAFRVVADNYVTDDSGTGIVHCAP 360

Query: 361  AFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEA------ 420
            AFGEDDYRVCIENQVI+KGENLIVAVDDDGCFTTKIT FSGRYVKDADKDIIEA      
Sbjct: 361  AFGEDDYRVCIENQVIHKGENLIVAVDDDGCFTTKITQFSGRYVKDADKDIIEAVKAQGR 420

Query: 421  -----------------------------------------------------VKEKRFH 480
                                                                 VKEKRFH
Sbjct: 421  LVKSGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEKLKEKLLENNEKTYWVPDFVKEKRFH 480

Query: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK------------ 540
            NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK            
Sbjct: 481  NWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVKVFDLHRHKIDHI 540

Query: 541  ------------------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600
                              VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT
Sbjct: 541  TIPSRRGPEYGVLQRVDDVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQT 600

Query: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA---- 660
            RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA    
Sbjct: 601  RGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGADALR 660

Query: 661  ------------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA 720
                                    VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA
Sbjct: 661  LYLINSPVVRAETLRFKKEGVFGVVRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQA 720

Query: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780
            TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK
Sbjct: 721  TLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRLK 780

Query: 781  GRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQE 840
            GRTGEEDCRIALSTLYHVLLTSC VMAPFTPFFTE LYQNLRKVSDGSEESIHYCSYPQE
Sbjct: 781  GRTGEEDCRIALSTLYHVLLTSCMVMAPFTPFFTEALYQNLRKVSDGSEESIHYCSYPQE 840

Query: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL----------------------- 900
            GGKRGERIEESVTRMMTIIDLARNIRERHNKPLK PL                       
Sbjct: 841  GGKRGERIEESVTRMMTIIDLARNIRERHNKPLKTPLREMIVVHPDKDFLDDIARKLREY 900

Query: 901  ----------------------------SLLGKRLWKTMGVVAKEVKAMSQEDILAFEKV 960
                                        S+LGKRL K MGVVAKEVKAMSQEDILAFEKV
Sbjct: 901  VLEELNIRSLIPCNDTLKYASLRAEPDFSVLGKRLGKAMGVVAKEVKAMSQEDILAFEKV 960

Query: 961  GEVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVARE 1020
            GEVTIATHCL+LS+IKVVRDF+RPDGMTDKE+DAAGDGDVLVILDLRPDESLFEAGVARE
Sbjct: 961  GEVTIATHCLRLSDIKVVRDFKRPDGMTDKEMDAAGDGDVLVILDLRPDESLFEAGVARE 1020

BLAST of HG10001777 vs. TAIR 10
Match: AT4G10320.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 1464.5 bits (3790), Expect = 0.0e+00
Identity = 767/1221 (62.82%), Postives = 866/1221 (70.93%), Query Frame = 0

Query: 1    MDEVCEGKDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHI 60
            M+EVCEGK+FSFP+ EE VL +W++I AF+TQL+RT +LPEYIFYDGPPFATGLPHYGHI
Sbjct: 1    MEEVCEGKEFSFPRQEEDVLSFWTEIDAFKTQLKRTENLPEYIFYDGPPFATGLPHYGHI 60

Query: 61   LAGTIKDIVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEE 120
            LAGTIKDIVTRYQ MTGHHVTRRFGWDCHGLPVENEID+KL IKRRD+V+KMGIDKYNEE
Sbjct: 61   LAGTIKDIVTRYQTMTGHHVTRRFGWDCHGLPVENEIDRKLNIKRRDEVIKMGIDKYNEE 120

Query: 121  CRSIVTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVM 180
            CRSIVTRYV EWEK+ITR GRWIDFKNDYKTMDL FMESVWWVFSQL+EK LVY+GFKVM
Sbjct: 121  CRSIVTRYVAEWEKVITRCGRWIDFKNDYKTMDLPFMESVWWVFSQLWEKNLVYRGFKVM 180

Query: 181  PYSTGCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCV 240
            PYSTGCKTPLSNFEAGQ+YK+VPDPEIMV FPV+GD  NAAFVAWTTTPWTLPSNLALCV
Sbjct: 181  PYSTGCKTPLSNFEAGQNYKEVPDPEIMVTFPVIGDQDNAAFVAWTTTPWTLPSNLALCV 240

Query: 241  NANFVYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPN--ANDAINKSNPKKKGSSNSK 300
            NA FVYVKVRNK +GKVY+VA+SRLSALP +K K    N  A D   K+NPK KG   +K
Sbjct: 241  NAKFVYVKVRNKNTGKVYIVAESRLSALPTDKPKAKLSNGPAGDT-KKANPKAKG---AK 300

Query: 301  TENAVEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCA 360
             E+A  DS+E+LE+  GASLVGKKY PLFDYF++FS  AFRVVAD YVTDDSGTGIVHCA
Sbjct: 301  PESAA-DSYEVLEKFNGASLVGKKYEPLFDYFSDFSSEAFRVVADDYVTDDSGTGIVHCA 360

Query: 361  PAFGEDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYVKDADKDIIEA----- 420
            PAFGEDDYRVC+ N++I KGENL+VAVDDDG FT +IT FSGRYVKDADKDIIEA     
Sbjct: 361  PAFGEDDYRVCLLNKIIKKGENLVVAVDDDGLFTERITHFSGRYVKDADKDIIEAVKAKG 420

Query: 421  ------------------------------------------------------VKEKRF 480
                                                                  VK+KRF
Sbjct: 421  RLVKTGSFTHSYPFCWRSDTPLIYRAVPSWFVRVEQLKEKLLKSNEQTEWVPGYVKDKRF 480

Query: 481  HNWLENARDWAVSRSRFWGTPLPVWVSEDGEEILVMDSIEKLEQLSGVK----------- 540
            HNWLENARDWA+SRSRFWGTPLP+W+S+DGEE+++MDS+EKLE+LSGVK           
Sbjct: 481  HNWLENARDWAISRSRFWGTPLPIWISDDGEEVVIMDSVEKLEKLSGVKVFDLHRHHIDH 540

Query: 541  -------------------VFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQ 600
                               VFDCWFESGSMPYAYIHYPFEN ELFEKNFPG FVAEGLDQ
Sbjct: 541  ITIPSSRGDEFGVLRRVEDVFDCWFESGSMPYAYIHYPFENKELFEKNFPGDFVAEGLDQ 600

Query: 601  TRGWFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYGA--- 660
            TRGWFYTLMVLSTALF KPAF+NLICNGLVLAEDGKKM+K+L+NYP P+E+I++YGA   
Sbjct: 601  TRGWFYTLMVLSTALFEKPAFKNLICNGLVLAEDGKKMAKKLRNYPPPLEVIDEYGADAV 660

Query: 661  -------------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSPVDQ 720
                                     V+DVFLPWYNAYRFLVQNAKRLE EG  PF P D 
Sbjct: 661  RLYLINSPVVRAEPLRFKKEGVLGVVKDVFLPWYNAYRFLVQNAKRLETEGGVPFVPTDL 720

Query: 721  ATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKFLDNLTNIYVRFNRKRL 780
            AT+Q S N+LDQWI+SATQSLV FVR+EMD YRLYTVVP LLKFLDNLTNIYVRFNRKRL
Sbjct: 721  ATIQ-SANILDQWIHSATQSLVRFVREEMDAYRLYTVVPRLLKFLDNLTNIYVRFNRKRL 780

Query: 781  KGRTGEEDCRIALSTLYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQ 840
            KGRTGE+DC  ALSTL++VLLTSCKVMAPFTPFFTE LYQNLRK   GSEES+HYCS P 
Sbjct: 781  KGRTGEDDCHTALSTLFNVLLTSCKVMAPFTPFFTETLYQNLRKACKGSEESVHYCSIPP 840

Query: 841  EGGKRGERIEESVTRMMTIIDLARNIRERHNKPLKAPL---------------------- 900
              G  GERIE SVTRMM IIDLARNIRER+  PLK PL                      
Sbjct: 841  REGMEGERIELSVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADFLNDITGVLRE 900

Query: 901  -----------------------------SLLGKRLWKTMGVVAKEVKAMSQEDILAFEK 960
                                         S+LGKRL K+MG+VAKEVK MSQ+DILAFE+
Sbjct: 901  YVLEELNVRSLVPCNDTLKYASLKAEPDFSVLGKRLGKSMGLVAKEVKEMSQKDILAFEE 960

Query: 961  VGEVTIATHCLKLSEIKVVRDFRRPDGMTDKEIDAAGDGDVLVILDLRPDESLFEAGVAR 1020
             GEVTIA H LK ++IK+VR F+RPD + + EID+AGDGDVLVILDLR D+SL EAG AR
Sbjct: 961  AGEVTIANHLLKETDIKIVRVFKRPDDLKENEIDSAGDGDVLVILDLRADDSLVEAGFAR 1020

BLAST of HG10001777 vs. TAIR 10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 226.9 bits (577), Expect = 7.5e-59
Identity = 216/884 (24.43%), Postives = 346/884 (39.14%), Query Frame = 0

Query: 16  EEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQVM 75
           E  + + W + + F+ ++    +   +I +DGPP+A G  H GH L   +KDI+ RY+++
Sbjct: 123 EPELQKLWEENQVFK-RVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLL 182

Query: 76  TGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTRYVGEWEKI 135
             + V    GWDCHGLP+E ++ Q L  + R ++  +   K   +        V    + 
Sbjct: 183 QNYKVQYVPGWDCHGLPIELKVLQSLDQEVRKELTPL---KLRAKAAKFAKATVKTQMES 242

Query: 136 ITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCKTPLSNFEA 195
             R G W D+ N Y T+D ++  +   VF Q+  KG +Y+G K + +S   +T L+  E 
Sbjct: 243 FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 302

Query: 196 GQSYKDVPDPEIMVAFPVLGDPQ---------NAAFVAWTTTPWTLPSNLALCVNANFVY 255
                 +    I   F ++G  +         N     WTTTPWT+P+N A+ VNA   Y
Sbjct: 303 EYPEGHI-SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 362

Query: 256 VKVRNKVSGKVYVVAKSRLSALP----VEKQKVNAPNANDAINKSNPKKKGSSNSKTENA 315
             V  +   +      S    +P      +QK+    A D +    P  +     K    
Sbjct: 363 SVVEVQSFSEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLV----PALEAKWGVK---- 422

Query: 316 VEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPAFG 375
                 I +  LG+ L   +Y    D  N    V   V+   Y+T +SGTG+VH AP  G
Sbjct: 423 ----LSISKTFLGSDLENCRYTHPID--NRDCPV---VIGGDYITTESGTGLVHTAPGHG 482

Query: 376 EDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYV------------------- 435
           ++DY   ++      G  L+  VDD+G FT +   F G  V                   
Sbjct: 483 QEDYATGLK-----YGLPLVSPVDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLV 542

Query: 436 ----------------------------------KDADKDIIEAVK------EKRFHNWL 495
                                             + A  D I  VK        R     
Sbjct: 543 MEESYAHKYPYDWRTKKPTIFRATEQWFASVEGFRTATMDAINNVKWVPHQAVNRISAMT 602

Query: 496 ENARDWAVSRSRFWGTPLPVW---------------------VSEDGEEILVMDSIEKL- 555
            +  DW +SR R WG P+P +                     +S+ G +     S+E L 
Sbjct: 603 SSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLL 662

Query: 556 ---------EQLSGVKVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRG 615
                    +   G    D WF+SGS  +A +    E +     +FP     EG DQ RG
Sbjct: 663 PEKYRDKAADYEKGTDTMDVWFDSGS-SWAGVLGKREGL-----SFPADVYLEGTDQHRG 722

Query: 616 WFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIND--------- 675
           WF + ++ S A  GK  +  +I +G VL E G KMSK L N   P  +I           
Sbjct: 723 WFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPA 782

Query: 676 YGA-------------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSP 735
           YGA                         + D++       R+L+ N     ++   P+  
Sbjct: 783 YGADVMRLWVSSVDYTGDVLIGPQILRQMSDIYRKLRGTLRYLLGNLHDWRVDNAVPY-- 842

Query: 736 VDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKF-LDNLTNIYVRFN 747
                  +   ++DQ      +++V  +++  + Y+ + +   + +F + +L+N Y    
Sbjct: 843 -------QDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFDIA 902

BLAST of HG10001777 vs. TAIR 10
Match: AT5G49030.3 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 226.9 bits (577), Expect = 7.5e-59
Identity = 216/884 (24.43%), Postives = 346/884 (39.14%), Query Frame = 0

Query: 16   EEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQVM 75
            E  + + W + + F+ ++    +   +I +DGPP+A G  H GH L   +KDI+ RY+++
Sbjct: 309  EPELQKLWEENQVFK-RVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLL 368

Query: 76   TGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTRYVGEWEKI 135
              + V    GWDCHGLP+E ++ Q L  + R ++  +   K   +        V    + 
Sbjct: 369  QNYKVQYVPGWDCHGLPIELKVLQSLDQEVRKELTPL---KLRAKAAKFAKATVKTQMES 428

Query: 136  ITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCKTPLSNFEA 195
              R G W D+ N Y T+D ++  +   VF Q+  KG +Y+G K + +S   +T L+  E 
Sbjct: 429  FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 488

Query: 196  GQSYKDVPDPEIMVAFPVLGDPQ---------NAAFVAWTTTPWTLPSNLALCVNANFVY 255
                  +    I   F ++G  +         N     WTTTPWT+P+N A+ VNA   Y
Sbjct: 489  EYPEGHI-SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 548

Query: 256  VKVRNKVSGKVYVVAKSRLSALP----VEKQKVNAPNANDAINKSNPKKKGSSNSKTENA 315
              V  +   +      S    +P      +QK+    A D +    P  +     K    
Sbjct: 549  SVVEVQSFSEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLV----PALEAKWGVK---- 608

Query: 316  VEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPAFG 375
                  I +  LG+ L   +Y    D  N    V   V+   Y+T +SGTG+VH AP  G
Sbjct: 609  ----LSISKTFLGSDLENCRYTHPID--NRDCPV---VIGGDYITTESGTGLVHTAPGHG 668

Query: 376  EDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYV------------------- 435
            ++DY   ++      G  L+  VDD+G FT +   F G  V                   
Sbjct: 669  QEDYATGLK-----YGLPLVSPVDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLV 728

Query: 436  ----------------------------------KDADKDIIEAVK------EKRFHNWL 495
                                              + A  D I  VK        R     
Sbjct: 729  MEESYAHKYPYDWRTKKPTIFRATEQWFASVEGFRTATMDAINNVKWVPHQAVNRISAMT 788

Query: 496  ENARDWAVSRSRFWGTPLPVW---------------------VSEDGEEILVMDSIEKL- 555
             +  DW +SR R WG P+P +                     +S+ G +     S+E L 
Sbjct: 789  SSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLL 848

Query: 556  ---------EQLSGVKVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRG 615
                     +   G    D WF+SGS  +A +    E +     +FP     EG DQ RG
Sbjct: 849  PEKYRDKAADYEKGTDTMDVWFDSGS-SWAGVLGKREGL-----SFPADVYLEGTDQHRG 908

Query: 616  WFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIND--------- 675
            WF + ++ S A  GK  +  +I +G VL E G KMSK L N   P  +I           
Sbjct: 909  WFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPA 968

Query: 676  YGA-------------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSP 735
            YGA                         + D++       R+L+ N     ++   P+  
Sbjct: 969  YGADVMRLWVSSVDYTGDVLIGPQILRQMSDIYRKLRGTLRYLLGNLHDWRVDNAVPY-- 1028

Query: 736  VDQATLQKSFNVLDQWINSATQSLVYFVRKEMDGYRLYTVVPYLLKF-LDNLTNIYVRFN 747
                   +   ++DQ      +++V  +++  + Y+ + +   + +F + +L+N Y    
Sbjct: 1029 -------QDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFDIA 1088

BLAST of HG10001777 vs. TAIR 10
Match: AT5G49030.2 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 219.5 bits (558), Expect = 1.2e-56
Identity = 203/827 (24.55%), Postives = 324/827 (39.18%), Query Frame = 0

Query: 16  EEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKDIVTRYQVM 75
           E  + + W + + F+ ++    +   +I +DGPP+A G  H GH L   +KDI+ RY+++
Sbjct: 123 EPELQKLWEENQVFK-RVSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLL 182

Query: 76  TGHHVTRRFGWDCHGLPVENEIDQKLGIKRRDDVLKMGIDKYNEECRSIVTRYVGEWEKI 135
             + V    GWDCHGLP+E ++ Q L  + R ++  +   K   +        V    + 
Sbjct: 183 QNYKVQYVPGWDCHGLPIELKVLQSLDQEVRKELTPL---KLRAKAAKFAKATVKTQMES 242

Query: 136 ITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYSTGCKTPLSNFEA 195
             R G W D+ N Y T+D ++  +   VF Q+  KG +Y+G K + +S   +T L+  E 
Sbjct: 243 FKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAEL 302

Query: 196 GQSYKDVPDPEIMVAFPVLGDPQ---------NAAFVAWTTTPWTLPSNLALCVNANFVY 255
                 +    I   F ++G  +         N     WTTTPWT+P+N A+ VNA   Y
Sbjct: 303 EYPEGHI-SKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQY 362

Query: 256 VKVRNKVSGKVYVVAKSRLSALP----VEKQKVNAPNANDAINKSNPKKKGSSNSKTENA 315
             V  +   +      S    +P      +QK+    A D +    P  +     K    
Sbjct: 363 SVVEVQSFSEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLV----PALEAKWGVK---- 422

Query: 316 VEDSFEILEEVLGASLVGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPAFG 375
                 I +  LG+ L   +Y    D  N    V   V+   Y+T +SGTG+VH AP  G
Sbjct: 423 ----LSISKTFLGSDLENCRYTHPID--NRDCPV---VIGGDYITTESGTGLVHTAPGHG 482

Query: 376 EDDYRVCIENQVINKGENLIVAVDDDGCFTTKITDFSGRYV------------------- 435
           ++DY   ++      G  L+  VDD+G FT +   F G  V                   
Sbjct: 483 QEDYATGLK-----YGLPLVSPVDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLV 542

Query: 436 ----------------------------------KDADKDIIEAVK------EKRFHNWL 495
                                             + A  D I  VK        R     
Sbjct: 543 MEESYAHKYPYDWRTKKPTIFRATEQWFASVEGFRTATMDAINNVKWVPHQAVNRISAMT 602

Query: 496 ENARDWAVSRSRFWGTPLPVW---------------------VSEDGEEILVMDSIEKL- 555
            +  DW +SR R WG P+P +                     +S+ G +     S+E L 
Sbjct: 603 SSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLL 662

Query: 556 ---------EQLSGVKVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRG 615
                    +   G    D WF+SGS  +A +    E +     +FP     EG DQ RG
Sbjct: 663 PEKYRDKAADYEKGTDTMDVWFDSGS-SWAGVLGKREGL-----SFPADVYLEGTDQHRG 722

Query: 616 WFYTLMVLSTALFGKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIIND--------- 675
           WF + ++ S A  GK  +  +I +G VL E G KMSK L N   P  +I           
Sbjct: 723 WFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPA 782

Query: 676 YGA-------------------------VRDVFLPWYNAYRFLVQNAKRLEIEGFAPFSP 704
           YGA                         + D++       R+L+ N     ++   P+  
Sbjct: 783 YGADVMRLWVSSVDYTGDVLIGPQILRQMSDIYRKLRGTLRYLLGNLHDWRVDNAVPY-- 842

BLAST of HG10001777 vs. TAIR 10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 155.6 bits (392), Expect = 2.1e-37
Identity = 190/832 (22.84%), Postives = 337/832 (40.50%), Query Frame = 0

Query: 8   KDFSFPKHEEGVLEYWSDIKAFETQLERTRDLPEYIFYDGPPFATGLPHYGHILAGTIKD 67
           K F F   EE + ++W     F+   ++      ++    PP  TG  H GH +  T++D
Sbjct: 71  KTFDF-SSEEKIYKWWESQGYFKPNFDQGGS--PFVIPMPPPNVTGSLHMGHAMFVTLED 130

Query: 68  IVTRYQVMTGHHVTRRFGWDCHGLPVENEIDQKL---GIKRRDDVLKMGIDKYNEECRSI 127
           I+ RY  M G       G D  G+  +  +++ L   GIKR D    +G D++ +     
Sbjct: 131 IMVRYNRMNGRPTLWLPGTDHAGIATQLVVEKMLASEGIKRVD----LGRDEFTKRVWEW 190

Query: 128 VTRYVGEWEKIITRTGRWIDFKNDYKTMDLKFMESVWWVFSQLYEKGLVYKGFKVMPYST 187
             +Y G     I R G   D+  +  T+D +   +V   F +L++KGL+Y+G  ++ +S 
Sbjct: 191 KEKYGGTITNQIKRLGASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGSYMVNWSP 250

Query: 188 GCKTPLSNFEAGQSYKDVPDPEIMVAFPVLGDPQNAAFVAWTTTPWTLPSNLALCVNANF 247
             +T +S+ E    Y + P     + + V G P        TT P TL  ++AL V+   
Sbjct: 251 NLQTAVSDLEV--EYSEEPGFLYHIKYRVAGSPDFLTIA--TTRPETLFGDVALAVHPE- 310

Query: 248 VYVKVRNKVSGKVYVVAKSRLSALPVEKQKVNAPNANDAINKSNPKKKGSSNSKTENAVE 307
                 +K  G+  +V  +    +P+              +K   K  G+   K     +
Sbjct: 311 --DDRYSKYVGQTAIVPMTYGRHVPI------------IADKYVDKDFGTGVLKISPGHD 370

Query: 308 DSFEILEEVLGASL--VGKKYVPLFDYFNEFSDVAFRVVADSYVTDDSGTGIVHCAPAFG 367
            +  +L   LG  +  V  K   L D    F  +    V +    D    G+     A  
Sbjct: 371 HNDYLLARKLGLPILNVMNKDATLNDVAGLFCGLDRFEVREKLWADLEEIGL-----AVK 430

Query: 368 EDDYRVCIENQVINKGENLIVAVDDDGCFT--TKITDFSGRYVKDADKDIIEAVKEKRFH 427
           ++ + + +      +G  +I  +     F     + + +   V++ +  II    EK ++
Sbjct: 431 KEPHTLRVPRS--QRGGEVIEPLVSKQWFVHMDPLAEKALLAVENKELTIIPERFEKIYN 490

Query: 428 NWLENARDWAVSRSRFWGTPLPVW--VSEDGEEILVM-----DSIEK--------LEQLS 487
           +WL N +DW +SR  +WG  +PVW  V +D EE  ++     +++EK        +E   
Sbjct: 491 HWLTNIKDWCISRQLWWGHRIPVWYVVGKDCEEDYIVAKSAEEALEKALEKYGKDVEIYQ 550

Query: 488 GVKVFDCWFESGSMPYAYIHYPFENVELFEKNFPGHFVAEGLDQTRGWFYTLMVLSTALF 547
              V D WF S   P++ + +P    + F   +P + +  G D    W   ++++     
Sbjct: 551 DPDVLDTWFSSSLWPFSTLGWPDVAAKDFNNFYPTNMLETGHDILFFWVARMVMMGIEFT 610

Query: 548 GKPAFRNLICNGLVLAEDGKKMSKRLKNYPSPMEIINDYG--AVRDVF------------ 607
           G   F ++  +GL+    G+KMSK L N   P++ I D+G  A+R               
Sbjct: 611 GTVPFSHVYLHGLIRDSQGRKMSKSLGNVIDPLDTIKDFGTDALRFTIALGTAGQDLNLS 670

Query: 608 ---LPWYNAYRFLVQNAKRLEIEGFAPFS-----------PVDQATLQKSFNVLDQWINS 667
              L    A+   + NA +  +      S            +D+     S  + + W  S
Sbjct: 671 TERLTANKAFTNKLWNAGKFVLHSLPSLSDTSAWENLLDLKLDKEETLLSLPLPECWAVS 730

Query: 668 ATQSLVYFVRKEMDGYRLYTVVPYLLKFL-DNLTNIYVRFNRKRLKGRTGEEDCRIALST 727
               L+  V    +      V      F   +  + Y+  ++ RL G  G      + + 
Sbjct: 731 KLHILIDSVTASYEKLFFGDVGRETYDFFWSDFADWYIEASKSRLYGSGGNSVSLASQAV 790

Query: 728 LYHVLLTSCKVMAPFTPFFTEVLYQNLRKVSDGSEESIHYCSYPQEGGKRGERIEESVTR 787
           L +V     K++ PF PF TE L+Q L       +E++    +PQ    R     ES+ R
Sbjct: 791 LLYVFENILKLLHPFMPFVTEDLWQAL----PYRKEALIVSPWPQNSLPRN---VESIKR 850