HG10001730 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10001730 vs. NCBI nr
Match: XP_038901730.1 (putative E3 ubiquitin-protein ligase RING1a [Benincasa hispida])

HSP 1 Score: 646.7 bits (1667), Expect = 1.3e-181
Identity = 331/365 (90.68%), Postives = 345/365 (94.52%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE 
Sbjct: 130 MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEE 189

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQN 120
                        IQESIAQIFQRQSEALSKKR+LGKDTAGVILTRSRRNHRN+HLRRQN
Sbjct: 190 LTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAGVILTRSRRNHRNLHLRRQN 249

Query: 121 GRGDEVSGYEDNEDDDDNNEGKDSSSADERFTEIRQRRKKRHPTIRSSQPSSSIANVDSG 180
           GRGD+VSGYEDNED+DDNNEGKDSSSADERFTE+RQRRKKRHPT+RSSQPSSSIA+VDSG
Sbjct: 250 GRGDDVSGYEDNEDEDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIAHVDSG 309

Query: 181 CTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHGSAGGSSNKSTRNSRLM 240
           CTDGCAESDLDMSRDNRTVSPGLVLN+EMLGWGRGGVRSN+RHGSAGGSSNKS+R+SRLM
Sbjct: 310 CTDGCAESDLDMSRDNRTVSPGLVLNSEMLGWGRGGVRSNSRHGSAGGSSNKSSRSSRLM 369

Query: 241 KLVNYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKTPLQ 300
           KLVNYLRGL+ENNNELDVHLLLISVDKESTPSL+QPHLHCRPSLTVKHLREYVSRKTPLQ
Sbjct: 370 KLVNYLRGLDENNNELDVHLLLISVDKESTPSLKQPHLHCRPSLTVKHLREYVSRKTPLQ 429

Query: 301 ADDVEILSVKGRHRTSNDQSTPSASVSIDGMSLVFDPLKYELQILEGEETLAGLQPDFIY 352
           ADDVEILSVKGRH TS DQSTP  S+SIDGMSLVFDPLKYELQILEGEETLAGLQPD IY
Sbjct: 430 ADDVEILSVKGRHSTSKDQSTPRTSISIDGMSLVFDPLKYELQILEGEETLAGLQPDCIY 489

BLAST of HG10001730 vs. NCBI nr
Match: KAG7010382.1 (putative E3 ubiquitin-protein ligase RING1a [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 625.2 bits (1611), Expect = 4.0e-175
Identity = 322/365 (88.22%), Postives = 336/365 (92.05%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE 
Sbjct: 148 MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEE 207

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQN 120
                        IQESIAQIFQRQSEALSKKR+LGKDTA V LTRSRRNHRN HLRRQN
Sbjct: 208 LTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAAVFLTRSRRNHRNAHLRRQN 267

Query: 121 GRGDEVSGYEDNEDDDDNNEGKDSSSADERFTEIRQRRKKRHPTIRSSQPSSSIANVDSG 180
           GRGDEV GYEDNED+DDNNEGKDSSSADERFTE+RQRRKKRHPT+RSSQPSSSIANVDSG
Sbjct: 268 GRGDEVLGYEDNEDEDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANVDSG 327

Query: 181 CTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHGSAGGSSNKSTRNSRLM 240
           CTDGCAES+LDMSR+NRTVSPGLVLN+EMLGWGRGGVRSN RH SAGGSSNKS+R+SRL 
Sbjct: 328 CTDGCAESELDMSRENRTVSPGLVLNSEMLGWGRGGVRSNARHSSAGGSSNKSSRSSRLT 387

Query: 241 KLVNYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKTPLQ 300
           KLVNYLRGLEENNNELDVHLLLISVDKES PSLQQPHLHCRPSLTV+HLREYVSRKTPLQ
Sbjct: 388 KLVNYLRGLEENNNELDVHLLLISVDKESAPSLQQPHLHCRPSLTVEHLREYVSRKTPLQ 447

Query: 301 ADDVEILSVKGRHRTSNDQSTPSASVSIDGMSLVFDPLKYELQILEGEETLAGLQPDFIY 352
           ADDVEILS+K RH TS+ Q+TPSAS+SIDG SLVFDPLKYELQ LEGEETLAGLQ D IY
Sbjct: 448 ADDVEILSMKARHSTSDVQTTPSASISIDGTSLVFDPLKYELQTLEGEETLAGLQADCIY 507

BLAST of HG10001730 vs. NCBI nr
Match: KAG6570524.1 (putative E3 ubiquitin-protein ligase RING1a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 625.2 bits (1611), Expect = 4.0e-175
Identity = 322/365 (88.22%), Postives = 336/365 (92.05%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE 
Sbjct: 130 MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEE 189

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQN 120
                        IQESIAQIFQRQSEALSKKR+LGKDTA V LTRSRRNHRN HLRRQN
Sbjct: 190 LTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAAVFLTRSRRNHRNAHLRRQN 249

Query: 121 GRGDEVSGYEDNEDDDDNNEGKDSSSADERFTEIRQRRKKRHPTIRSSQPSSSIANVDSG 180
           GRGDEV GYEDNED+DDNNEGKDSSSADERFTE+RQRRKKRHPT+RSSQPSSSIANVDSG
Sbjct: 250 GRGDEVLGYEDNEDEDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANVDSG 309

Query: 181 CTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHGSAGGSSNKSTRNSRLM 240
           CTDGCAES+LDMSR+NRTVSPGLVLN+EMLGWGRGGVRSN RH SAGGSSNKS+R+SRL 
Sbjct: 310 CTDGCAESELDMSRENRTVSPGLVLNSEMLGWGRGGVRSNARHSSAGGSSNKSSRSSRLT 369

Query: 241 KLVNYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKTPLQ 300
           KLVNYLRGLEENNNELDVHLLLISVDKES PSLQQPHLHCRPSLTV+HLREYVSRKTPLQ
Sbjct: 370 KLVNYLRGLEENNNELDVHLLLISVDKESAPSLQQPHLHCRPSLTVEHLREYVSRKTPLQ 429

Query: 301 ADDVEILSVKGRHRTSNDQSTPSASVSIDGMSLVFDPLKYELQILEGEETLAGLQPDFIY 352
           ADDVEILS+K RH TS+ Q+TPSAS+SIDG SLVFDPLKYELQ LEGEETLAGLQ D IY
Sbjct: 430 ADDVEILSMKARHSTSDVQTTPSASISIDGTSLVFDPLKYELQTLEGEETLAGLQADCIY 489

BLAST of HG10001730 vs. NCBI nr
Match: XP_022985957.1 (putative E3 ubiquitin-protein ligase RING1a isoform X2 [Cucurbita maxima])

HSP 1 Score: 624.0 bits (1608), Expect = 9.0e-175
Identity = 321/365 (87.95%), Postives = 336/365 (92.05%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALI+ALYPDIDKYEEE 
Sbjct: 130 MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALISALYPDIDKYEEEE 189

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQN 120
                        IQESIAQIFQRQSEALSKKR+LGKDTA V LTRSRRNHRN HLRRQN
Sbjct: 190 LTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAAVFLTRSRRNHRNAHLRRQN 249

Query: 121 GRGDEVSGYEDNEDDDDNNEGKDSSSADERFTEIRQRRKKRHPTIRSSQPSSSIANVDSG 180
           GRGDEV GYEDNED+DDNNEGKDSSSADERFTE+RQRRKKRHPT+RSSQPSSSIANVDSG
Sbjct: 250 GRGDEVLGYEDNEDEDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANVDSG 309

Query: 181 CTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHGSAGGSSNKSTRNSRLM 240
           CTDGCAES+LDMSR+NRTVSPGLVLN+EMLGWGRGGVRSN RH SAGGSSNKS+R+SRL 
Sbjct: 310 CTDGCAESELDMSRENRTVSPGLVLNSEMLGWGRGGVRSNARHSSAGGSSNKSSRSSRLT 369

Query: 241 KLVNYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKTPLQ 300
           KLVNYLRGLEENNNELDVHLLLISVDKES PSLQQPHLHCRPSLTV+HLREYVSRKTPLQ
Sbjct: 370 KLVNYLRGLEENNNELDVHLLLISVDKESAPSLQQPHLHCRPSLTVEHLREYVSRKTPLQ 429

Query: 301 ADDVEILSVKGRHRTSNDQSTPSASVSIDGMSLVFDPLKYELQILEGEETLAGLQPDFIY 352
           ADDVEILS+K RH TS+ Q+TPSAS+SIDG SLVFDPLKYELQ LEGEETLAGLQ D IY
Sbjct: 430 ADDVEILSMKARHSTSDVQTTPSASISIDGTSLVFDPLKYELQTLEGEETLAGLQADCIY 489

BLAST of HG10001730 vs. NCBI nr
Match: XP_022985956.1 (putative E3 ubiquitin-protein ligase RING1a isoform X1 [Cucurbita maxima])

HSP 1 Score: 624.0 bits (1608), Expect = 9.0e-175
Identity = 321/365 (87.95%), Postives = 336/365 (92.05%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALI+ALYPDIDKYEEE 
Sbjct: 137 MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALISALYPDIDKYEEEE 196

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQN 120
                        IQESIAQIFQRQSEALSKKR+LGKDTA V LTRSRRNHRN HLRRQN
Sbjct: 197 LTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAAVFLTRSRRNHRNAHLRRQN 256

Query: 121 GRGDEVSGYEDNEDDDDNNEGKDSSSADERFTEIRQRRKKRHPTIRSSQPSSSIANVDSG 180
           GRGDEV GYEDNED+DDNNEGKDSSSADERFTE+RQRRKKRHPT+RSSQPSSSIANVDSG
Sbjct: 257 GRGDEVLGYEDNEDEDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANVDSG 316

Query: 181 CTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHGSAGGSSNKSTRNSRLM 240
           CTDGCAES+LDMSR+NRTVSPGLVLN+EMLGWGRGGVRSN RH SAGGSSNKS+R+SRL 
Sbjct: 317 CTDGCAESELDMSRENRTVSPGLVLNSEMLGWGRGGVRSNARHSSAGGSSNKSSRSSRLT 376

Query: 241 KLVNYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKTPLQ 300
           KLVNYLRGLEENNNELDVHLLLISVDKES PSLQQPHLHCRPSLTV+HLREYVSRKTPLQ
Sbjct: 377 KLVNYLRGLEENNNELDVHLLLISVDKESAPSLQQPHLHCRPSLTVEHLREYVSRKTPLQ 436

Query: 301 ADDVEILSVKGRHRTSNDQSTPSASVSIDGMSLVFDPLKYELQILEGEETLAGLQPDFIY 352
           ADDVEILS+K RH TS+ Q+TPSAS+SIDG SLVFDPLKYELQ LEGEETLAGLQ D IY
Sbjct: 437 ADDVEILSMKARHSTSDVQTTPSASISIDGTSLVFDPLKYELQTLEGEETLAGLQADCIY 496

BLAST of HG10001730 vs. ExPASy Swiss-Prot
Match: Q9FKW0 (Putative E3 ubiquitin-protein ligase RING1a OS=Arabidopsis thaliana OX=3702 GN=RING1A PE=1 SV=2)

HSP 1 Score: 302.8 bits (774), Expect = 6.0e-81
Identity = 187/375 (49.87%), Postives = 252/375 (67.20%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECPACR HCASRRSLRDDP +DALIAAL+ +ID YEEE 
Sbjct: 150 MECLHRFCRECIDKSMRLGNNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEE 209

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGV-----ILTRSRRNHRNVH 120
                        IQ SIAQI QRQSEAL K+R LGK+ A +     I + SRR   + +
Sbjct: 210 LAFHEDEMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRN 269

Query: 121 LRRQNGRGDEVSGYEDNEDDDDNNEGKD-SSSADERFTEIRQRRKKRHPTIRSSQ-PSSS 180
           + +QN        +ED+++DD+NN G+D  SS+DER TE+RQ+++++  T RS+Q PSSS
Sbjct: 270 MEQQNAS----EAHEDDDNDDNNNRGRDKDSSSDERGTEVRQKKRRKRSTSRSTQHPSSS 329

Query: 181 IANVDSGCTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHG-SAGGSSNK 240
            AN ++G    CA++D ++ RD + +SPGLV N E+L WGRGG RSNTRHG +  G S+K
Sbjct: 330 GANKNNG---NCADNDTEVYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSK 389

Query: 241 STRNSRLMKLVNYLR-GLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLRE 300
           S RN+R+ KLV YLR  ++ ++ ELD+H+ L+S+D +  P L QP+L CRP+L VK LRE
Sbjct: 390 SVRNARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLRE 449

Query: 301 YVSRKTPLQADDVEILSVKGRHRTSND---QSTPSASVSIDGMSLVFDPLKYELQILEGE 350
           +V+ +  L+ ++VE+L    R R   D   ++ P+ + +    S      K E+  LE  
Sbjct: 450 FVALQIHLKTEEVELLVT--RRRVGEDAAIENLPAVTPASAAAS------KDEMLSLEDN 509

BLAST of HG10001730 vs. ExPASy Swiss-Prot
Match: Q0WX00 (Putative E3 ubiquitin-protein ligase RING1b OS=Arabidopsis thaliana OX=3702 GN=RING1B PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 6.7e-72
Identity = 182/366 (49.73%), Postives = 230/366 (62.84%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECP CR HCASRRSLRDDPN+DALIAAL+ +IDK+EEE 
Sbjct: 117 MECLHRFCRECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEE 176

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQN 120
                        IQ SIAQ+ QRQS+AL K++ +GK TA  IL+RSRR+      RR+N
Sbjct: 177 LNFRQDDEARNKQIQASIAQVSQRQSKALVKRKSVGKGTA--ILSRSRRSGGGSR-RRRN 236

Query: 121 GRGDEVSGYEDNEDDDDNNEGKDSSSADERFTEIRQRRKKRHPTIRSSQPSSSIANVDSG 180
            R  E    E N+DDD N  GKDSSS DE      ++RKKR  T    QPSSS AN +  
Sbjct: 237 CRNIEQDTSEANDDDDQNKRGKDSSS-DE---PCERQRKKRSAT----QPSSSNANNNDN 296

Query: 181 CTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHGSAGGSSNKSTRNSRLM 240
           C     E      RD+R +SP LV N+E++ WGRGG RSNTR G+       S RN+RL 
Sbjct: 297 CAGNGTEQ--THQRDSRVISPVLVWNSELIAWGRGGTRSNTRQGN-NNQGAISKRNARLK 356

Query: 241 KLVNYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKTPLQ 300
           +LV YL  LE N+ ELD+HL L+S+D E   +L +P+L  RP+L VK LREYV+R   L+
Sbjct: 357 RLVEYLGSLEGNSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREYVARHLKLK 416

Query: 301 ADDVEILSVKGRHRTSNDQSTPSASVSIDGMSLVFDPLKYE-LQILEGEETLAGLQPDFI 352
           A++VE+L                  VS DG +++ +    E +Q L+ +ET+A L+ D I
Sbjct: 417 AEEVELL------------------VSKDGDTVIGNKTSTEKMQSLQDDETVAKLKVDCI 450

BLAST of HG10001730 vs. ExPASy Swiss-Prot
Match: Q803I4 (E3 ubiquitin-protein ligase RING2 OS=Danio rerio OX=7955 GN=rnf2 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 2.2e-14
Identity = 49/119 (41.18%), Postives = 68/119 (57.14%), Query Frame = 0

Query: 2   ECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEIQ 61
           ECLHRFC +CI  ++R GN ECP CR    S+RSLR DPN+DALI+ +YP  D+YE   +
Sbjct: 66  ECLHRFCADCIITALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQE 125

Query: 62  ESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQNGRGDEVSGYEDNED 121
             +A+I    S+  +++ L      G+ +    R  R    + +NG     SG EDN D
Sbjct: 126 RVLARI----SKHNNQQALSHSIEEGLKIQAMNRLQRGKKHQIENG-----SGAEDNGD 175

BLAST of HG10001730 vs. ExPASy Swiss-Prot
Match: Q99496 (E3 ubiquitin-protein ligase RING2 OS=Homo sapiens OX=9606 GN=RNF2 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 9.2e-13
Identity = 46/135 (34.07%), Postives = 75/135 (55.56%), Query Frame = 0

Query: 2   ECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEIQ 61
           ECLHRFC +CI  ++R GN ECP CR    S+RSLR DPN+DALI+ +YP  D+YE   +
Sbjct: 66  ECLHRFCADCIITALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQE 125

Query: 62  ESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQNGRGDEVSGYEDNEDD 121
             +A+I +  ++    + L      G+ +    R  R    + +NG G E +G   +  +
Sbjct: 126 RVLARINKHNNQ----QALSHSIEEGLKIQAMNRLQRGKKQQIENGSGAEDNGDSSHCSN 185

Query: 122 DDNNEGKDSSSADER 137
              +  +++  +++R
Sbjct: 186 ASTHSNQEAGPSNKR 196

BLAST of HG10001730 vs. ExPASy Swiss-Prot
Match: Q9CQJ4 (E3 ubiquitin-protein ligase RING2 OS=Mus musculus OX=10090 GN=Rnf2 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 9.2e-13
Identity = 46/135 (34.07%), Postives = 75/135 (55.56%), Query Frame = 0

Query: 2   ECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEIQ 61
           ECLHRFC +CI  ++R GN ECP CR    S+RSLR DPN+DALI+ +YP  D+YE   +
Sbjct: 66  ECLHRFCADCIITALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSRDEYEAHQE 125

Query: 62  ESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQNGRGDEVSGYEDNEDD 121
             +A+I +  ++    + L      G+ +    R  R    + +NG G E +G   +  +
Sbjct: 126 RVLARINKHNNQ----QALSHSIEEGLKIQAMNRLQRGKKQQIENGSGAEDNGDSSHCSN 185

Query: 122 DDNNEGKDSSSADER 137
              +  +++  +++R
Sbjct: 186 ASTHSNQEAGPSNKR 196

BLAST of HG10001730 vs. ExPASy TrEMBL
Match: A0A6J1J6A5 (putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483842 PE=4 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 4.4e-175
Identity = 321/365 (87.95%), Postives = 336/365 (92.05%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALI+ALYPDIDKYEEE 
Sbjct: 130 MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALISALYPDIDKYEEEE 189

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQN 120
                        IQESIAQIFQRQSEALSKKR+LGKDTA V LTRSRRNHRN HLRRQN
Sbjct: 190 LTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAAVFLTRSRRNHRNAHLRRQN 249

Query: 121 GRGDEVSGYEDNEDDDDNNEGKDSSSADERFTEIRQRRKKRHPTIRSSQPSSSIANVDSG 180
           GRGDEV GYEDNED+DDNNEGKDSSSADERFTE+RQRRKKRHPT+RSSQPSSSIANVDSG
Sbjct: 250 GRGDEVLGYEDNEDEDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANVDSG 309

Query: 181 CTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHGSAGGSSNKSTRNSRLM 240
           CTDGCAES+LDMSR+NRTVSPGLVLN+EMLGWGRGGVRSN RH SAGGSSNKS+R+SRL 
Sbjct: 310 CTDGCAESELDMSRENRTVSPGLVLNSEMLGWGRGGVRSNARHSSAGGSSNKSSRSSRLT 369

Query: 241 KLVNYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKTPLQ 300
           KLVNYLRGLEENNNELDVHLLLISVDKES PSLQQPHLHCRPSLTV+HLREYVSRKTPLQ
Sbjct: 370 KLVNYLRGLEENNNELDVHLLLISVDKESAPSLQQPHLHCRPSLTVEHLREYVSRKTPLQ 429

Query: 301 ADDVEILSVKGRHRTSNDQSTPSASVSIDGMSLVFDPLKYELQILEGEETLAGLQPDFIY 352
           ADDVEILS+K RH TS+ Q+TPSAS+SIDG SLVFDPLKYELQ LEGEETLAGLQ D IY
Sbjct: 430 ADDVEILSMKARHSTSDVQTTPSASISIDGTSLVFDPLKYELQTLEGEETLAGLQADCIY 489

BLAST of HG10001730 vs. ExPASy TrEMBL
Match: A0A6J1J9P4 (putative E3 ubiquitin-protein ligase RING1a isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483842 PE=4 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 4.4e-175
Identity = 321/365 (87.95%), Postives = 336/365 (92.05%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALI+ALYPDIDKYEEE 
Sbjct: 137 MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALISALYPDIDKYEEEE 196

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQN 120
                        IQESIAQIFQRQSEALSKKR+LGKDTA V LTRSRRNHRN HLRRQN
Sbjct: 197 LTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAAVFLTRSRRNHRNAHLRRQN 256

Query: 121 GRGDEVSGYEDNEDDDDNNEGKDSSSADERFTEIRQRRKKRHPTIRSSQPSSSIANVDSG 180
           GRGDEV GYEDNED+DDNNEGKDSSSADERFTE+RQRRKKRHPT+RSSQPSSSIANVDSG
Sbjct: 257 GRGDEVLGYEDNEDEDDNNEGKDSSSADERFTEVRQRRKKRHPTVRSSQPSSSIANVDSG 316

Query: 181 CTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHGSAGGSSNKSTRNSRLM 240
           CTDGCAES+LDMSR+NRTVSPGLVLN+EMLGWGRGGVRSN RH SAGGSSNKS+R+SRL 
Sbjct: 317 CTDGCAESELDMSRENRTVSPGLVLNSEMLGWGRGGVRSNARHSSAGGSSNKSSRSSRLT 376

Query: 241 KLVNYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKTPLQ 300
           KLVNYLRGLEENNNELDVHLLLISVDKES PSLQQPHLHCRPSLTV+HLREYVSRKTPLQ
Sbjct: 377 KLVNYLRGLEENNNELDVHLLLISVDKESAPSLQQPHLHCRPSLTVEHLREYVSRKTPLQ 436

Query: 301 ADDVEILSVKGRHRTSNDQSTPSASVSIDGMSLVFDPLKYELQILEGEETLAGLQPDFIY 352
           ADDVEILS+K RH TS+ Q+TPSAS+SIDG SLVFDPLKYELQ LEGEETLAGLQ D IY
Sbjct: 437 ADDVEILSMKARHSTSDVQTTPSASISIDGTSLVFDPLKYELQTLEGEETLAGLQADCIY 496

BLAST of HG10001730 vs. ExPASy TrEMBL
Match: A0A1S3BJA1 (putative E3 ubiquitin-protein ligase RING1b isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490502 PE=4 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 5.7e-175
Identity = 325/365 (89.04%), Postives = 338/365 (92.60%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE 
Sbjct: 129 MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEE 188

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQN 120
                        IQESIAQIFQRQSEALSKKR+LGKDTAGVILTRSRRNHRNVHLRRQN
Sbjct: 189 LTFHEEDRNRNKQIQESIAQIFQRQSEALSKKRMLGKDTAGVILTRSRRNHRNVHLRRQN 248

Query: 121 GRGDEVSGYEDNEDDDDNNEGKDSSSADERFTEIRQRRKKRHPTIRSSQPSSSIANVDSG 180
           GRG+EVSGYEDNEDDDDNNEGKDSSSADERFTE+RQRRKKRHPT+RSSQPSSSIAN+DSG
Sbjct: 249 GRGEEVSGYEDNEDDDDNNEGKDSSSADERFTEVRQRRKKRHPTLRSSQPSSSIANIDSG 308

Query: 181 CTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHGSAGGSSNKSTRNSRLM 240
             DGCAESDLDMSR+NRTVSPGLVLNTEMLGWGRGGVRSN+RHGSAGGS NKS+R+SRLM
Sbjct: 309 --DGCAESDLDMSRENRTVSPGLVLNTEMLGWGRGGVRSNSRHGSAGGSGNKSSRSSRLM 368

Query: 241 KLVNYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKTPLQ 300
           KLV YLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKT LQ
Sbjct: 369 KLVKYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKTSLQ 428

Query: 301 ADDVEILSVKGRHRTSNDQSTPSASVSIDGMSLVFDPLKYELQILEGEETLAGLQPDFIY 352
           ADDVEILS+KGR RTSN+QST SAS+SIDGMSLVFDP KYELQ LEGE+ LAGLQ D  Y
Sbjct: 429 ADDVEILSLKGRPRTSNEQSTRSASISIDGMSLVFDPFKYELQSLEGEKNLAGLQSDCTY 488

BLAST of HG10001730 vs. ExPASy TrEMBL
Match: A0A6J1FTH1 (putative E3 ubiquitin-protein ligase RING1b isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448324 PE=4 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.3e-174
Identity = 321/365 (87.95%), Postives = 335/365 (91.78%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE 
Sbjct: 137 MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEE 196

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQN 120
                        IQESIAQIFQRQSEALSKKR+LGKDTA V LTRSRRNHRN HLRRQN
Sbjct: 197 LTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAAVFLTRSRRNHRNAHLRRQN 256

Query: 121 GRGDEVSGYEDNEDDDDNNEGKDSSSADERFTEIRQRRKKRHPTIRSSQPSSSIANVDSG 180
           GRGDEV GYEDNED+DDNNEGKDSSSADERFTE+RQRRKKRHPT+ SSQPSSSIANVDSG
Sbjct: 257 GRGDEVLGYEDNEDEDDNNEGKDSSSADERFTEVRQRRKKRHPTVPSSQPSSSIANVDSG 316

Query: 181 CTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHGSAGGSSNKSTRNSRLM 240
           CTDGCAES+LDMSR+NRTVSPGLVLN+EMLGWGRGGVRSN RH SAGGSSNKS+R+SRL 
Sbjct: 317 CTDGCAESELDMSRENRTVSPGLVLNSEMLGWGRGGVRSNARHSSAGGSSNKSSRSSRLT 376

Query: 241 KLVNYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKTPLQ 300
           KLVNYLRGLEENNNELDVHLLLISVDKES PSLQQPHLHCRPSLTV+HLREYVSRKTPLQ
Sbjct: 377 KLVNYLRGLEENNNELDVHLLLISVDKESAPSLQQPHLHCRPSLTVEHLREYVSRKTPLQ 436

Query: 301 ADDVEILSVKGRHRTSNDQSTPSASVSIDGMSLVFDPLKYELQILEGEETLAGLQPDFIY 352
           ADDVEILS+K RH TS+ Q+TPSAS+SIDG SLVFDPLKYELQ LEGEETLAGLQ D IY
Sbjct: 437 ADDVEILSMKARHSTSDVQTTPSASISIDGTSLVFDPLKYELQTLEGEETLAGLQADCIY 496

BLAST of HG10001730 vs. ExPASy TrEMBL
Match: A0A6J1FY08 (putative E3 ubiquitin-protein ligase RING1a isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448324 PE=4 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.3e-174
Identity = 321/365 (87.95%), Postives = 335/365 (91.78%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE 
Sbjct: 130 MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEEE 189

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQN 120
                        IQESIAQIFQRQSEALSKKR+LGKDTA V LTRSRRNHRN HLRRQN
Sbjct: 190 LTFHEEERNRNKQIQESIAQIFQRQSEALSKKRILGKDTAAVFLTRSRRNHRNAHLRRQN 249

Query: 121 GRGDEVSGYEDNEDDDDNNEGKDSSSADERFTEIRQRRKKRHPTIRSSQPSSSIANVDSG 180
           GRGDEV GYEDNED+DDNNEGKDSSSADERFTE+RQRRKKRHPT+ SSQPSSSIANVDSG
Sbjct: 250 GRGDEVLGYEDNEDEDDNNEGKDSSSADERFTEVRQRRKKRHPTVPSSQPSSSIANVDSG 309

Query: 181 CTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHGSAGGSSNKSTRNSRLM 240
           CTDGCAES+LDMSR+NRTVSPGLVLN+EMLGWGRGGVRSN RH SAGGSSNKS+R+SRL 
Sbjct: 310 CTDGCAESELDMSRENRTVSPGLVLNSEMLGWGRGGVRSNARHSSAGGSSNKSSRSSRLT 369

Query: 241 KLVNYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKTPLQ 300
           KLVNYLRGLEENNNELDVHLLLISVDKES PSLQQPHLHCRPSLTV+HLREYVSRKTPLQ
Sbjct: 370 KLVNYLRGLEENNNELDVHLLLISVDKESAPSLQQPHLHCRPSLTVEHLREYVSRKTPLQ 429

Query: 301 ADDVEILSVKGRHRTSNDQSTPSASVSIDGMSLVFDPLKYELQILEGEETLAGLQPDFIY 352
           ADDVEILS+K RH TS+ Q+TPSAS+SIDG SLVFDPLKYELQ LEGEETLAGLQ D IY
Sbjct: 430 ADDVEILSMKARHSTSDVQTTPSASISIDGTSLVFDPLKYELQTLEGEETLAGLQADCIY 489

BLAST of HG10001730 vs. TAIR 10
Match: AT5G44280.1 (RING 1A )

HSP 1 Score: 302.8 bits (774), Expect = 4.3e-82
Identity = 187/375 (49.87%), Postives = 252/375 (67.20%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECPACR HCASRRSLRDDP +DALIAAL+ +ID YEEE 
Sbjct: 150 MECLHRFCRECIDKSMRLGNNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEE 209

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGV-----ILTRSRRNHRNVH 120
                        IQ SIAQI QRQSEAL K+R LGK+ A +     I + SRR   + +
Sbjct: 210 LAFHEDEMARNKQIQASIAQISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRN 269

Query: 121 LRRQNGRGDEVSGYEDNEDDDDNNEGKD-SSSADERFTEIRQRRKKRHPTIRSSQ-PSSS 180
           + +QN        +ED+++DD+NN G+D  SS+DER TE+RQ+++++  T RS+Q PSSS
Sbjct: 270 MEQQNAS----EAHEDDDNDDNNNRGRDKDSSSDERGTEVRQKKRRKRSTSRSTQHPSSS 329

Query: 181 IANVDSGCTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHG-SAGGSSNK 240
            AN ++G    CA++D ++ RD + +SPGLV N E+L WGRGG RSNTRHG +  G S+K
Sbjct: 330 GANKNNG---NCADNDTEVYRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSK 389

Query: 241 STRNSRLMKLVNYLR-GLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLRE 300
           S RN+R+ KLV YLR  ++ ++ ELD+H+ L+S+D +  P L QP+L CRP+L VK LRE
Sbjct: 390 SVRNARVNKLVEYLRSSVDGSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLRE 449

Query: 301 YVSRKTPLQADDVEILSVKGRHRTSND---QSTPSASVSIDGMSLVFDPLKYELQILEGE 350
           +V+ +  L+ ++VE+L    R R   D   ++ P+ + +    S      K E+  LE  
Sbjct: 450 FVALQIHLKTEEVELLVT--RRRVGEDAAIENLPAVTPASAAAS------KDEMLSLEDN 509

BLAST of HG10001730 vs. TAIR 10
Match: AT1G03770.1 (RING 1B )

HSP 1 Score: 272.7 bits (696), Expect = 4.7e-73
Identity = 182/366 (49.73%), Postives = 230/366 (62.84%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECP CR HCASRRSLRDDPN+DALIAAL+ +IDK+EEE 
Sbjct: 117 MECLHRFCRECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEE 176

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQN 120
                        IQ SIAQ+ QRQS+AL K++ +GK TA  IL+RSRR+      RR+N
Sbjct: 177 LNFRQDDEARNKQIQASIAQVSQRQSKALVKRKSVGKGTA--ILSRSRRSGGGSR-RRRN 236

Query: 121 GRGDEVSGYEDNEDDDDNNEGKDSSSADERFTEIRQRRKKRHPTIRSSQPSSSIANVDSG 180
            R  E    E N+DDD N  GKDSSS DE      ++RKKR  T    QPSSS AN +  
Sbjct: 237 CRNIEQDTSEANDDDDQNKRGKDSSS-DE---PCERQRKKRSAT----QPSSSNANNNDN 296

Query: 181 CTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHGSAGGSSNKSTRNSRLM 240
           C     E      RD+R +SP LV N+E++ WGRGG RSNTR G+       S RN+RL 
Sbjct: 297 CAGNGTEQ--THQRDSRVISPVLVWNSELIAWGRGGTRSNTRQGN-NNQGAISKRNARLK 356

Query: 241 KLVNYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKTPLQ 300
           +LV YL  LE N+ ELD+HL L+S+D E   +L +P+L  RP+L VK LREYV+R   L+
Sbjct: 357 RLVEYLGSLEGNSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREYVARHLKLK 416

Query: 301 ADDVEILSVKGRHRTSNDQSTPSASVSIDGMSLVFDPLKYE-LQILEGEETLAGLQPDFI 352
           A++VE+L                  VS DG +++ +    E +Q L+ +ET+A L+ D I
Sbjct: 417 AEEVELL------------------VSKDGDTVIGNKTSTEKMQSLQDDETVAKLKVDCI 450

BLAST of HG10001730 vs. TAIR 10
Match: AT1G03770.3 (RING 1B )

HSP 1 Score: 266.2 bits (679), Expect = 4.4e-71
Identity = 182/372 (48.92%), Postives = 230/372 (61.83%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECP CR HCASRRSLRDDPN+DALIAAL+ +IDK+EEE 
Sbjct: 117 MECLHRFCRECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEE 176

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQN 120
                        IQ SIAQ+ QRQS+AL K++ +GK TA  IL+RSRR+      RR+N
Sbjct: 177 LNFRQDDEARNKQIQASIAQVSQRQSKALVKRKSVGKGTA--ILSRSRRSGGGSR-RRRN 236

Query: 121 GRGDEVSGYEDNEDDDDNNEGKDSSSADERFTEIRQRRKKRHPTIRSSQPSSSIANVDSG 180
            R  E    E N+DDD N  GKDSSS DE      ++RKKR  T    QPSSS AN +  
Sbjct: 237 CRNIEQDTSEANDDDDQNKRGKDSSS-DE---PCERQRKKRSAT----QPSSSNANNNDN 296

Query: 181 CTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHGSAGGSSNKSTRNSRLM 240
           C     E      RD+R +SP LV N+E++ WGRGG RSNTR G+       S RN+RL 
Sbjct: 297 CAGNGTEQ--THQRDSRVISPVLVWNSELIAWGRGGTRSNTRQGN-NNQGAISKRNARLK 356

Query: 241 KLVNYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLRE------YVS 300
           +LV YL  LE N+ ELD+HL L+S+D E   +L +P+L  RP+L VK LRE      YV+
Sbjct: 357 RLVEYLGSLEGNSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREVSSLPLYVA 416

Query: 301 RKTPLQADDVEILSVKGRHRTSNDQSTPSASVSIDGMSLVFDPLKYE-LQILEGEETLAG 352
           R   L+A++VE+L                  VS DG +++ +    E +Q L+ +ET+A 
Sbjct: 417 RHLKLKAEEVELL------------------VSKDGDTVIGNKTSTEKMQSLQDDETVAK 456

BLAST of HG10001730 vs. TAIR 10
Match: AT1G03770.2 (RING 1B )

HSP 1 Score: 266.2 bits (679), Expect = 4.4e-71
Identity = 182/372 (48.92%), Postives = 230/372 (61.83%), Query Frame = 0

Query: 1   MECLHRFCRECIDKSMRLGNNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE- 60
           MECLHRFCRECIDKSMRLGNNECP CR HCASRRSLRDDPN+DALIAAL+ +IDK+EEE 
Sbjct: 119 MECLHRFCRECIDKSMRLGNNECPTCRKHCASRRSLRDDPNFDALIAALFKNIDKFEEEE 178

Query: 61  -------------IQESIAQIFQRQSEALSKKRLLGKDTAGVILTRSRRNHRNVHLRRQN 120
                        IQ SIAQ+ QRQS+AL K++ +GK TA  IL+RSRR+      RR+N
Sbjct: 179 LNFRQDDEARNKQIQASIAQVSQRQSKALVKRKSVGKGTA--ILSRSRRSGGGSR-RRRN 238

Query: 121 GRGDEVSGYEDNEDDDDNNEGKDSSSADERFTEIRQRRKKRHPTIRSSQPSSSIANVDSG 180
            R  E    E N+DDD N  GKDSSS DE      ++RKKR  T    QPSSS AN +  
Sbjct: 239 CRNIEQDTSEANDDDDQNKRGKDSSS-DE---PCERQRKKRSAT----QPSSSNANNNDN 298

Query: 181 CTDGCAESDLDMSRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHGSAGGSSNKSTRNSRLM 240
           C     E      RD+R +SP LV N+E++ WGRGG RSNTR G+       S RN+RL 
Sbjct: 299 CAGNGTEQ--THQRDSRVISPVLVWNSELIAWGRGGTRSNTRQGN-NNQGAISKRNARLK 358

Query: 241 KLVNYLRGLEENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLRE------YVS 300
           +LV YL  LE N+ ELD+HL L+S+D E   +L +P+L  RP+L VK LRE      YV+
Sbjct: 359 RLVEYLGSLEGNSVELDIHLKLVSLDTEGLLNLHEPYLCFRPTLLVKQLREVSSLPLYVA 418

Query: 301 RKTPLQADDVEILSVKGRHRTSNDQSTPSASVSIDGMSLVFDPLKYE-LQILEGEETLAG 352
           R   L+A++VE+L                  VS DG +++ +    E +Q L+ +ET+A 
Sbjct: 419 RHLKLKAEEVELL------------------VSKDGDTVIGNKTSTEKMQSLQDDETVAK 458

BLAST of HG10001730 vs. TAIR 10
Match: AT5G44280.2 (RING 1A )

HSP 1 Score: 261.2 bits (666), Expect = 1.4e-69
Identity = 168/356 (47.19%), Postives = 233/356 (65.45%), Query Frame = 0

Query: 20  NNECPACRTHCASRRSLRDDPNYDALIAALYPDIDKYEEE--------------IQESIA 79
           NNECPACR HCASRRSLRDDP +DALIAAL+ +ID YEEE              IQ SIA
Sbjct: 172 NNECPACRKHCASRRSLRDDPKFDALIAALFTNIDSYEEEELAFHEDEMARNKQIQASIA 231

Query: 80  QIFQRQSEALSKKRLLGKDTAGV-----ILTRSRRNHRNVHLRRQNGRGDEVSGYEDNED 139
           QI QRQSEAL K+R LGK+ A +     I + SRR   + ++ +QN        +ED+++
Sbjct: 232 QISQRQSEALVKRRSLGKEAAVLMRSPRIASGSRRRRNSRNMEQQNAS----EAHEDDDN 291

Query: 140 DDDNNEGKD-SSSADERFTEIRQRRKKRHPTIRSSQ-PSSSIANVDSGCTDGCAESDLDM 199
           DD+NN G+D  SS+DER TE+RQ+++++  T RS+Q PSSS AN ++G    CA++D ++
Sbjct: 292 DDNNNRGRDKDSSSDERGTEVRQKKRRKRSTSRSTQHPSSSGANKNNG---NCADNDTEV 351

Query: 200 SRDNRTVSPGLVLNTEMLGWGRGGVRSNTRHG-SAGGSSNKSTRNSRLMKLVNYLR-GLE 259
            RD + +SPGLV N E+L WGRGG RSNTRHG +  G S+KS RN+R+ KLV YLR  ++
Sbjct: 352 YRDTKGISPGLVWNPEILAWGRGGTRSNTRHGNNTSGGSSKSVRNARVNKLVEYLRSSVD 411

Query: 260 ENNNELDVHLLLISVDKESTPSLQQPHLHCRPSLTVKHLREYVSRKTPLQADDVEILSVK 319
            ++ ELD+H+ L+S+D +  P L QP+L CRP+L VK LRE+V+ +  L+ ++VE+L   
Sbjct: 412 GSSVELDIHVKLVSLDTKCIPDLPQPYLCCRPTLLVKQLREFVALQIHLKTEEVELLVT- 471

Query: 320 GRHRTSND---QSTPSASVSIDGMSLVFDPLKYELQILEGEETLAGLQPDFIYSRD 350
            R R   D   ++ P+ + +    S      K E+  LE  ETL+ L+ DFI S +
Sbjct: 472 -RRRVGEDAAIENLPAVTPASAAAS------KDEMLSLEDNETLSRLKIDFISSHE 512