HG10001669 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10001669
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionDNA-directed RNA polymerase III subunit rpc6
LocationChr09: 19326027 .. 19326746 (-)
RNA-Seq ExpressionHG10001669
SyntenyHG10001669
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCAAATCACAAGGGCCTTCATCACTCAAAAGAAAAAGACCAGACTCAGCTCATGGCTCTGGAGGAGCTCTCATTGAGCATGAAAAAGTGCTCCATGATCTGATTTGCAGCAAACAAAGCATGGGGATTTGGATTGGAGACATTAGAAGAGAAACAAAATATGCTGACAGTGTGGTAACAAAATCACTCAAGTCCCTTCAAGCTAGAGGCCTGATTAAACTAGTTGAACACTATCAAAACAAAGGGAAGAAATTCTACCTGTCAAAAGATTTCGAACCATCGAAAGAACTGACTGGCGGTAATTGGTATAGCGACGGGAAGCTCGACCAAGAATACATCAATACTCTTAAAGGGTTCTGTGCTAGAATGATACAGAAGTTGAAGTTGGCTACAATTGAAGGAATTGTAGATTCTTTCAAAAAGAGTGGAGCATCCAAAGTGGACTTGTCTAAGGAGCAAATTGAAGAGATTGTAAATGCTTTGGTGTTGGACAATGAAGTTATGGAGGTAAAGAGCACTGGTTCAGGGGAGTTTGATTTTATTCCTGTTGGGAAGATTTGTTATAAGTTTGCTAAAGGAGGTGTTAAAGGGGAGCCCAAAACAGGAGCCATGGCTTCTATTCCTTGTGGAGTTTGTCCAAGGATTAGTCATTGTACCCCAGATGGCATCATTTCCCCTAGAACTTGTGTGTATTACACCAAGTGGTTAGATTTTTAA

mRNA sequence

ATGAGCAAATCACAAGGGCCTTCATCACTCAAAAGAAAAAGACCAGACTCAGCTCATGGCTCTGGAGGAGCTCTCATTGAGCATGAAAAAGTGCTCCATGATCTGATTTGCAGCAAACAAAGCATGGGGATTTGGATTGGAGACATTAGAAGAGAAACAAAATATGCTGACAGTGTGGTAACAAAATCACTCAAGTCCCTTCAAGCTAGAGGCCTGATTAAACTAGTTGAACACTATCAAAACAAAGGGAAGAAATTCTACCTGTCAAAAGATTTCGAACCATCGAAAGAACTGACTGGCGGTAATTGGTATAGCGACGGGAAGCTCGACCAAGAATACATCAATACTCTTAAAGGGTTCTGTGCTAGAATGATACAGAAGTTGAAGTTGGCTACAATTGAAGGAATTGTAGATTCTTTCAAAAAGAGTGGAGCATCCAAAGTGGACTTGTCTAAGGAGCAAATTGAAGAGATTGTAAATGCTTTGGTGTTGGACAATGAAGTTATGGAGGTAAAGAGCACTGGTTCAGGGGAGTTTGATTTTATTCCTGTTGGGAAGATTTGTTATAAGTTTGCTAAAGGAGGTGTTAAAGGGGAGCCCAAAACAGGAGCCATGGCTTCTATTCCTTGTGGAGTTTGTCCAAGGATTAGTCATTGTACCCCAGATGGCATCATTTCCCCTAGAACTTGTGTGTATTACACCAAGTGGTTAGATTTTTAA

Coding sequence (CDS)

ATGAGCAAATCACAAGGGCCTTCATCACTCAAAAGAAAAAGACCAGACTCAGCTCATGGCTCTGGAGGAGCTCTCATTGAGCATGAAAAAGTGCTCCATGATCTGATTTGCAGCAAACAAAGCATGGGGATTTGGATTGGAGACATTAGAAGAGAAACAAAATATGCTGACAGTGTGGTAACAAAATCACTCAAGTCCCTTCAAGCTAGAGGCCTGATTAAACTAGTTGAACACTATCAAAACAAAGGGAAGAAATTCTACCTGTCAAAAGATTTCGAACCATCGAAAGAACTGACTGGCGGTAATTGGTATAGCGACGGGAAGCTCGACCAAGAATACATCAATACTCTTAAAGGGTTCTGTGCTAGAATGATACAGAAGTTGAAGTTGGCTACAATTGAAGGAATTGTAGATTCTTTCAAAAAGAGTGGAGCATCCAAAGTGGACTTGTCTAAGGAGCAAATTGAAGAGATTGTAAATGCTTTGGTGTTGGACAATGAAGTTATGGAGGTAAAGAGCACTGGTTCAGGGGAGTTTGATTTTATTCCTGTTGGGAAGATTTGTTATAAGTTTGCTAAAGGAGGTGTTAAAGGGGAGCCCAAAACAGGAGCCATGGCTTCTATTCCTTGTGGAGTTTGTCCAAGGATTAGTCATTGTACCCCAGATGGCATCATTTCCCCTAGAACTTGTGTGTATTACACCAAGTGGTTAGATTTTTAA

Protein sequence

MSKSQGPSSLKRKRPDSAHGSGGALIEHEKVLHDLICSKQSMGIWIGDIRRETKYADSVVTKSLKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLKGFCARMIQKLKLATIEGIVDSFKKSGASKVDLSKEQIEEIVNALVLDNEVMEVKSTGSGEFDFIPVGKICYKFAKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKWLDF
Homology
BLAST of HG10001669 vs. NCBI nr
Match: XP_022149571.1 (DNA-directed RNA polymerase III subunit rpc6 isoform X1 [Momordica charantia] >XP_022149572.1 DNA-directed RNA polymerase III subunit rpc6 isoform X1 [Momordica charantia])

HSP 1 Score: 450.7 bits (1158), Expect = 8.0e-123
Identity = 218/240 (90.83%), Postives = 236/240 (98.33%), Query Frame = 0

Query: 1   MSKSQGPSSLKRKRPDSAHGSGGALIEHEKVLHDLICSKQSMGIWIGDIRRETKYADSVV 60
           MS+SQGPSSLKRKRPDS+  SGGALIEHEKVL+DLICSKQSMGIWIGDI+RETKYADS+V
Sbjct: 1   MSRSQGPSSLKRKRPDSS--SGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIV 60

Query: 61  TKSLKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLKGF 120
           TKSLK+LQA+G+IKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYS+GKLD+EYINTLKGF
Sbjct: 61  TKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGF 120

Query: 121 CARMIQKLKLATIEGIVDSFKKSGASKVDLSKEQIEEIVNALVLDNEVMEVKSTGSGEFD 180
           C RMIQK+K+ATIEGIVDSFKKSGASKVDLS+EQI+EIVNALVLDNEVMEVKSTGSGEFD
Sbjct: 121 CGRMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFD 180

Query: 181 FIPVGKICYK-FAKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKWLDF 240
           FIP+GKICYK +AKGGVKGEPKTGAMASIPCGVCPRISHCTP+G+ISPRTCVYYTKWLDF
Sbjct: 181 FIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 238

BLAST of HG10001669 vs. NCBI nr
Match: XP_022970903.1 (DNA-directed RNA polymerase III subunit rpc6 [Cucurbita maxima])

HSP 1 Score: 445.7 bits (1145), Expect = 2.6e-121
Identity = 214/239 (89.54%), Postives = 231/239 (96.65%), Query Frame = 0

Query: 1   MSKSQGPSSLKRKRPDSAHGSGGALIEHEKVLHDLICSKQSMGIWIGDIRRETKYADSVV 60
           MS+SQGPSSLKRKRP+ AHG GGALIEHEKVL+DLICSKQSMGIWIGD++RETKY D+VV
Sbjct: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60

Query: 61  TKSLKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLKGF 120
           TKSLK+LQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLD+EYI+TLKGF
Sbjct: 61  TKSLKALQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120

Query: 121 CARMIQKLKLATIEGIVDSFKKSGASKVDLSKEQIEEIVNALVLDNEVMEVKSTGSGEFD 180
           CARMIQK+K+ATIEGIVD+FKKSGASKVDLS+EQIEEIVNALVLDNEVMEVKSTG+GEFD
Sbjct: 121 CARMIQKMKVATIEGIVDAFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180

Query: 181 FIPVGKICYKFAKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKWLDF 240
           FIPVGKICYK    GVKGEPKTGAMAS PCGVCPRI+HCTP+GIISPRTCVYYTKWL+F
Sbjct: 181 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 239

BLAST of HG10001669 vs. NCBI nr
Match: KAG6604735.1 (DNA-directed RNA polymerase III subunit rpc6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 445.3 bits (1144), Expect = 3.4e-121
Identity = 214/239 (89.54%), Postives = 230/239 (96.23%), Query Frame = 0

Query: 1   MSKSQGPSSLKRKRPDSAHGSGGALIEHEKVLHDLICSKQSMGIWIGDIRRETKYADSVV 60
           MS+SQGPSSLKRKRP+ AHG GGALIEHEKVL+DLICSKQSMGIWIGD++RETKY D+VV
Sbjct: 89  MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 148

Query: 61  TKSLKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLKGF 120
           TKSLK+LQ RGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLD+EYI+TLKGF
Sbjct: 149 TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 208

Query: 121 CARMIQKLKLATIEGIVDSFKKSGASKVDLSKEQIEEIVNALVLDNEVMEVKSTGSGEFD 180
           CARMIQK+K+ATIEGIVDSFKKSGASKVDLS+EQIEEIVNALVLDNEVMEVKSTG+GEFD
Sbjct: 209 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 268

Query: 181 FIPVGKICYKFAKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKWLDF 240
           FIPVGKICYK    GVKGEPKTGAMAS PCGVCPRI+HCTP+GIISPRTCVYYTKWL+F
Sbjct: 269 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 327

BLAST of HG10001669 vs. NCBI nr
Match: XP_022946967.1 (DNA-directed RNA polymerase III subunit rpc6 [Cucurbita moschata] >XP_023532566.1 DNA-directed RNA polymerase III subunit rpc6 [Cucurbita pepo subsp. pepo] >KAG7034865.1 DNA-directed RNA polymerase III subunit rpc6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 445.3 bits (1144), Expect = 3.4e-121
Identity = 214/239 (89.54%), Postives = 230/239 (96.23%), Query Frame = 0

Query: 1   MSKSQGPSSLKRKRPDSAHGSGGALIEHEKVLHDLICSKQSMGIWIGDIRRETKYADSVV 60
           MS+SQGPSSLKRKRP+ AHG GGALIEHEKVL+DLICSKQSMGIWIGD++RETKY D+VV
Sbjct: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60

Query: 61  TKSLKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLKGF 120
           TKSLK+LQ RGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLD+EYI+TLKGF
Sbjct: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120

Query: 121 CARMIQKLKLATIEGIVDSFKKSGASKVDLSKEQIEEIVNALVLDNEVMEVKSTGSGEFD 180
           CARMIQK+K+ATIEGIVDSFKKSGASKVDLS+EQIEEIVNALVLDNEVMEVKSTG+GEFD
Sbjct: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180

Query: 181 FIPVGKICYKFAKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKWLDF 240
           FIPVGKICYK    GVKGEPKTGAMAS PCGVCPRI+HCTP+GIISPRTCVYYTKWL+F
Sbjct: 181 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 239

BLAST of HG10001669 vs. NCBI nr
Match: XP_038900531.1 (LOW QUALITY PROTEIN: DNA-directed RNA polymerase III subunit RPC6-like [Benincasa hispida])

HSP 1 Score: 421.0 bits (1081), Expect = 6.8e-114
Identity = 211/238 (88.66%), Postives = 218/238 (91.60%), Query Frame = 0

Query: 1   MSKSQGPSSLKRKRPDSAHGSGGALIEHEKVLHDLICSKQSMGIWIGDIRRETKYADSVV 60
           MSK QGPSSLKRKR DSAHG GGALIEHEKVLHDLICSKQ+MGIWIGDIR+ETKYAD VV
Sbjct: 1   MSKPQGPSSLKRKRLDSAHGFGGALIEHEKVLHDLICSKQNMGIWIGDIRKETKYADGVV 60

Query: 61  TKSLKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLKGF 120
            K LKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLKGF
Sbjct: 61  RKLLKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLKGF 120

Query: 121 CARMIQKLKLATIEGIVDSFKKSGASKVDLSKEQIEEIVNALVLDNEVMEVKSTGSGEFD 180
           CARMIQKLK++TIEG V SFKKSG SKVDLS+EQIEEIVNALVLDNEVMEVK TGS EFD
Sbjct: 121 CARMIQKLKVSTIEGTVXSFKKSGTSKVDLSREQIEEIVNALVLDNEVMEVKRTGSREFD 180

Query: 181 FIPVGKICYKFAKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTK-WL 238
           FIPV KICYK     VKGEPKTGAMASIPCGVCPRISHCT DGIIS RTC+YYTK W+
Sbjct: 181 FIPVRKICYKCYAKDVKGEPKTGAMASIPCGVCPRISHCTLDGIISHRTCMYYTKCWI 238

BLAST of HG10001669 vs. ExPASy Swiss-Prot
Match: Q86JM3 (DNA-directed RNA polymerase III subunit rpc6 OS=Dictyostelium discoideum OX=44689 GN=polr3f PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.0e-24
Identity = 69/240 (28.75%), Postives = 120/240 (50.00%), Query Frame = 0

Query: 1   MSKSQGPSSLKRKRPDSAHGSGGALIEHEKVLHDLICSKQSMGIWIGDIRRETKYADSVV 60
           +  S   +S K   P+      G  +E + +++ LI +  + G W  +++  + Y    +
Sbjct: 52  IQNSDETTSYKEVNPEDQAKFRGLGVE-DFLIYQLIEASSNTGAWTRELKNSSGYQQVQI 111

Query: 61  TKSLKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLKGF 120
           TK LK+L++R LIK V+  Q+  KK Y+  + EPS+E+TGG  YSD   D +YI  +K  
Sbjct: 112 TKILKTLESRKLIKSVKSIQSGRKKVYMLYNMEPSREITGGQLYSDQSYDHQYIQIMKMH 171

Query: 121 CARMIQKLKLATIEGIVDSFKK--SGASKVDLSKEQIEEIVNALVLDNEVMEVKSTGSGE 180
               +       +  I+   +K    A+   L  E I+ +VN ++ D ++ E++ T  G 
Sbjct: 172 IKTFVDNKGAVDLADIITYLRKVAEEATSQSLGPEDIQALVNTVIYDGDIEEMRDTRMGA 231

Query: 181 FDFIPVGKICYKFAKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKWLD 239
                 G I YK  K  +          ++PCG CP    C+ +G++SP+ CVY+ ++L+
Sbjct: 232 MLGRRSG-ILYKPTKTPI----PVNNFCNMPCGNCPVFDICSDNGLVSPKRCVYFKQYLE 285

BLAST of HG10001669 vs. ExPASy Swiss-Prot
Match: Q2T9S3 (DNA-directed RNA polymerase III subunit RPC6 OS=Bos taurus OX=9913 GN=POLR3F PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.8e-24
Identity = 72/229 (31.44%), Postives = 111/229 (48.47%), Query Frame = 0

Query: 29  EKVLHDLICSKQSMGIWIGDIRRETKYADSVVTKSLKSLQARGLIKLVEHYQNKGKKFYL 88
           EK+++ +I    + GIW  DIR ++    + + K LK+L+++ LIK V+      KK Y+
Sbjct: 92  EKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYM 151

Query: 89  SKDFEPSKELTGGNWYSDGKLDQEYINTLKGFCARMIQKLKLATIEGIVD-------SFK 148
             + +P + +TGG WYSD   + E++  L   C + +Q       E   +       SF 
Sbjct: 152 LYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQNKAETARESKQNPMIQRNSSFA 211

Query: 149 KS----------GASKVDLSKEQIEEIVNALVLDNEV-MEVKSTGSGEFDFIPVGKICYK 208
            S          G SKV+LS E IE I+N L+ D +V M + +   G    +      Y+
Sbjct: 212 SSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYR 271

Query: 209 FAKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKWLDF 240
                +   P TG +   PCG+CP    C   G ISP  C+Y T+WL+F
Sbjct: 272 AVSPII---PPTG-LVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF 316

BLAST of HG10001669 vs. ExPASy Swiss-Prot
Match: Q9H1D9 (DNA-directed RNA polymerase III subunit RPC6 OS=Homo sapiens OX=9606 GN=POLR3F PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.8e-24
Identity = 72/229 (31.44%), Postives = 111/229 (48.47%), Query Frame = 0

Query: 29  EKVLHDLICSKQSMGIWIGDIRRETKYADSVVTKSLKSLQARGLIKLVEHYQNKGKKFYL 88
           EK+++ +I    + GIW  DIR ++    + + K LK+L+++ LIK V+      KK Y+
Sbjct: 92  EKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYM 151

Query: 89  SKDFEPSKELTGGNWYSDGKLDQEYINTLKGFCARMIQKLKLATIEGIVD-------SFK 148
             + +P + +TGG WYSD   + E++  L   C + +Q       E   +       SF 
Sbjct: 152 LYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPMIQRNSSFA 211

Query: 149 KS----------GASKVDLSKEQIEEIVNALVLDNEV-MEVKSTGSGEFDFIPVGKICYK 208
            S          G SKV+LS E IE I+N L+ D +V M + +   G    +      Y+
Sbjct: 212 SSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYR 271

Query: 209 FAKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKWLDF 240
                +   P TG +   PCG+CP    C   G ISP  C+Y T+WL+F
Sbjct: 272 AVNPII---PPTG-LVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLEF 316

BLAST of HG10001669 vs. ExPASy Swiss-Prot
Match: Q921X6 (DNA-directed RNA polymerase III subunit RPC6 OS=Mus musculus OX=10090 GN=Polr3f PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.3e-24
Identity = 72/229 (31.44%), Postives = 112/229 (48.91%), Query Frame = 0

Query: 29  EKVLHDLICSKQSMGIWIGDIRRETKYADSVVTKSLKSLQARGLIKLVEHYQNKGKKFYL 88
           EK+++ +I    + GIW  DIR ++    + + K LK+L+++ LIK V+      KK Y+
Sbjct: 92  EKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYM 151

Query: 89  SKDFEPSKELTGGNWYSDGKLDQEYINTLKGFCARMIQKLKLATIEGIVD-------SFK 148
             + +P + +TGG WYSD   + E++  L   C + +Q       E   +       SF 
Sbjct: 152 LYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPVIQRNSSFA 211

Query: 149 KS----------GASKVDLSKEQIEEIVNALVLDNEV-MEVKSTGSGEFDFIPVGKICYK 208
            S          G SKV+LS E IE I+N L+ D +V M + +   G    +      Y+
Sbjct: 212 SSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYR 271

Query: 209 FAKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKWLDF 240
                +   P TG + + PCG+CP    C   G ISP  C+Y T+WL+F
Sbjct: 272 AVNPIL---PPTGVVRA-PCGLCPVFEDCHEGGEISPSNCIYMTEWLEF 316

BLAST of HG10001669 vs. ExPASy Swiss-Prot
Match: Q9VD25 (Probable DNA-directed RNA polymerase III subunit RPC6 OS=Drosophila melanogaster OX=7227 GN=CG5380 PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 6.4e-22
Identity = 64/227 (28.19%), Postives = 104/227 (45.81%), Query Frame = 0

Query: 29  EKVLHDLICSKQSMGIWIGDIRRETKYADSVVTKSLKSLQARGLIKLVEHYQNKGKKFYL 88
           EKV++ ++    + GIWI DIR ++      + K LK+L+ + LIK V+      KK Y+
Sbjct: 80  EKVVYGIVEEGGNKGIWIRDIRMKSNLNMIQLNKILKNLETKKLIKAVKSVNASKKKVYM 139

Query: 89  SKDFEPSKELTGGNWYSDGKLDQEYINTLKGFCARMIQ-----------------KLKLA 148
             + EP   +TGG WY D   + E+++ L   C R +Q                 ++   
Sbjct: 140 LYNLEPDLSITGGAWYQDQDFEVEFVDVLNQQCLRFLQMKRDSAEKKREGPLAFKQMSCC 199

Query: 149 TIEGIVDSFKKSGASKVDLSKEQIEEIVNALVLDNEVMEVKSTGSGEFDFIPVGKICYKF 208
           T+  +       G SKV+L++  +E I+  +V D     V+            G   Y+ 
Sbjct: 200 TVNEVQKFISDLGISKVNLAEADLETILKTVVYDGNAERVRQQD---------GSFVYRA 259

Query: 209 AKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKWLD 239
               +   P TG +  +PCG+CP I +C+  G ++  TC Y   WLD
Sbjct: 260 VNAPL---PPTG-LVQMPCGICPVIKNCSNCGDVTAITCEYMRDWLD 293

BLAST of HG10001669 vs. ExPASy TrEMBL
Match: A0A6J1D8D1 (DNA-directed RNA polymerase III subunit rpc6 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017972 PE=3 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 3.9e-123
Identity = 218/240 (90.83%), Postives = 236/240 (98.33%), Query Frame = 0

Query: 1   MSKSQGPSSLKRKRPDSAHGSGGALIEHEKVLHDLICSKQSMGIWIGDIRRETKYADSVV 60
           MS+SQGPSSLKRKRPDS+  SGGALIEHEKVL+DLICSKQSMGIWIGDI+RETKYADS+V
Sbjct: 1   MSRSQGPSSLKRKRPDSS--SGGALIEHEKVLYDLICSKQSMGIWIGDIKRETKYADSIV 60

Query: 61  TKSLKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLKGF 120
           TKSLK+LQA+G+IKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYS+GKLD+EYINTLKGF
Sbjct: 61  TKSLKALQAKGMIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSEGKLDREYINTLKGF 120

Query: 121 CARMIQKLKLATIEGIVDSFKKSGASKVDLSKEQIEEIVNALVLDNEVMEVKSTGSGEFD 180
           C RMIQK+K+ATIEGIVDSFKKSGASKVDLS+EQI+EIVNALVLDNEVMEVKSTGSGEFD
Sbjct: 121 CGRMIQKMKVATIEGIVDSFKKSGASKVDLSREQIQEIVNALVLDNEVMEVKSTGSGEFD 180

Query: 181 FIPVGKICYK-FAKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKWLDF 240
           FIP+GKICYK +AKGGVKGEPKTGAMASIPCGVCPRISHCTP+G+ISPRTCVYYTKWLDF
Sbjct: 181 FIPIGKICYKCYAKGGVKGEPKTGAMASIPCGVCPRISHCTPNGVISPRTCVYYTKWLDF 238

BLAST of HG10001669 vs. ExPASy TrEMBL
Match: A0A6J1I599 (DNA-directed RNA polymerase III subunit rpc6 OS=Cucurbita maxima OX=3661 GN=LOC111469737 PE=3 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.3e-121
Identity = 214/239 (89.54%), Postives = 231/239 (96.65%), Query Frame = 0

Query: 1   MSKSQGPSSLKRKRPDSAHGSGGALIEHEKVLHDLICSKQSMGIWIGDIRRETKYADSVV 60
           MS+SQGPSSLKRKRP+ AHG GGALIEHEKVL+DLICSKQSMGIWIGD++RETKY D+VV
Sbjct: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60

Query: 61  TKSLKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLKGF 120
           TKSLK+LQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLD+EYI+TLKGF
Sbjct: 61  TKSLKALQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120

Query: 121 CARMIQKLKLATIEGIVDSFKKSGASKVDLSKEQIEEIVNALVLDNEVMEVKSTGSGEFD 180
           CARMIQK+K+ATIEGIVD+FKKSGASKVDLS+EQIEEIVNALVLDNEVMEVKSTG+GEFD
Sbjct: 121 CARMIQKMKVATIEGIVDAFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180

Query: 181 FIPVGKICYKFAKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKWLDF 240
           FIPVGKICYK    GVKGEPKTGAMAS PCGVCPRI+HCTP+GIISPRTCVYYTKWL+F
Sbjct: 181 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 239

BLAST of HG10001669 vs. ExPASy TrEMBL
Match: A0A6J1G553 (DNA-directed RNA polymerase III subunit rpc6 OS=Cucurbita moschata OX=3662 GN=LOC111450988 PE=3 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 1.6e-121
Identity = 214/239 (89.54%), Postives = 230/239 (96.23%), Query Frame = 0

Query: 1   MSKSQGPSSLKRKRPDSAHGSGGALIEHEKVLHDLICSKQSMGIWIGDIRRETKYADSVV 60
           MS+SQGPSSLKRKRP+ AHG GGALIEHEKVL+DLICSKQSMGIWIGD++RETKY D+VV
Sbjct: 1   MSRSQGPSSLKRKRPEPAHGHGGALIEHEKVLYDLICSKQSMGIWIGDMKRETKYPDNVV 60

Query: 61  TKSLKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLKGF 120
           TKSLK+LQ RGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLD+EYI+TLKGF
Sbjct: 61  TKSLKALQGRGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDREYISTLKGF 120

Query: 121 CARMIQKLKLATIEGIVDSFKKSGASKVDLSKEQIEEIVNALVLDNEVMEVKSTGSGEFD 180
           CARMIQK+K+ATIEGIVDSFKKSGASKVDLS+EQIEEIVNALVLDNEVMEVKSTG+GEFD
Sbjct: 121 CARMIQKMKVATIEGIVDSFKKSGASKVDLSREQIEEIVNALVLDNEVMEVKSTGAGEFD 180

Query: 181 FIPVGKICYKFAKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKWLDF 240
           FIPVGKICYK    GVKGEPKTGAMAS PCGVCPRI+HCTP+GIISPRTCVYYTKWL+F
Sbjct: 181 FIPVGKICYKSYAKGVKGEPKTGAMASTPCGVCPRINHCTPNGIISPRTCVYYTKWLEF 239

BLAST of HG10001669 vs. ExPASy TrEMBL
Match: A0A1S3BL06 (DNA-directed RNA polymerase III subunit rpc6-like OS=Cucumis melo OX=3656 GN=LOC103490992 PE=3 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.4e-104
Identity = 191/243 (78.60%), Postives = 219/243 (90.12%), Query Frame = 0

Query: 1   MSKSQGPSSLKRKRPD--SAHGSGGALIEHEKVLHDLICSKQSMGIWIGDIRRETKYADS 60
           MSKSQGPSSLKRKRPD  + + SGGALI  EKVL+DLICSKQSMGI   D++RETK+A+S
Sbjct: 1   MSKSQGPSSLKRKRPDLGTKNSSGGALIGQEKVLYDLICSKQSMGICTFDMKRETKFAES 60

Query: 61  VVTKSLKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLK 120
           VVTKSLKSLQ +GLIK+V+HYQNKGKKFYLS DFEP+KELTGGNWY DG+LD++YINTLK
Sbjct: 61  VVTKSLKSLQTKGLIKIVQHYQNKGKKFYLSNDFEPAKELTGGNWYRDGELDKDYINTLK 120

Query: 121 GFCARMIQKLKLATIEGIVDSFKKSGASKVDLSKEQIEEIVNALVLDNEVMEVKSTGSGE 180
             CARMIQ+ KL TIEGIV+SFK+SGA KV+LS+EQIEEIVNALVLDN+VMEVKS+G GE
Sbjct: 121 QVCARMIQRKKLMTIEGIVESFKQSGAFKVELSREQIEEIVNALVLDNQVMEVKSSGFGE 180

Query: 181 FDFIPVGKICYKFAK--GGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKW 240
           F+FIP+GKICYK+    GG+KGEPK GAMASIPCGVCPRI++CTP+GIISP TCVYYTKW
Sbjct: 181 FEFIPIGKICYKYCTQGGGLKGEPKIGAMASIPCGVCPRITYCTPNGIISPATCVYYTKW 240

BLAST of HG10001669 vs. ExPASy TrEMBL
Match: A0A5D3D7H7 (DNA-directed RNA polymerase III subunit rpc6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00330 PE=3 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.4e-104
Identity = 191/243 (78.60%), Postives = 219/243 (90.12%), Query Frame = 0

Query: 1   MSKSQGPSSLKRKRPD--SAHGSGGALIEHEKVLHDLICSKQSMGIWIGDIRRETKYADS 60
           MSKSQGPSSLKRKRPD  + + SGGALI  EKVL+DLICSKQSMGI   D++RETK+A+S
Sbjct: 5   MSKSQGPSSLKRKRPDLGTKNSSGGALIGQEKVLYDLICSKQSMGICTFDMKRETKFAES 64

Query: 61  VVTKSLKSLQARGLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLK 120
           VVTKSLKSLQ +GLIK+V+HYQNKGKKFYLS DFEP+KELTGGNWY DG+LD++YINTLK
Sbjct: 65  VVTKSLKSLQTKGLIKIVQHYQNKGKKFYLSNDFEPAKELTGGNWYRDGELDKDYINTLK 124

Query: 121 GFCARMIQKLKLATIEGIVDSFKKSGASKVDLSKEQIEEIVNALVLDNEVMEVKSTGSGE 180
             CARMIQ+ KL TIEGIV+SFK+SGA KV+LS+EQIEEIVNALVLDN+VMEVKS+G GE
Sbjct: 125 QVCARMIQRKKLMTIEGIVESFKQSGAFKVELSREQIEEIVNALVLDNQVMEVKSSGFGE 184

Query: 181 FDFIPVGKICYKFAK--GGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKW 240
           F+FIP+GKICYK+    GG+KGEPK GAMASIPCGVCPRI++CTP+GIISP TCVYYTKW
Sbjct: 185 FEFIPIGKICYKYCTQGGGLKGEPKIGAMASIPCGVCPRITYCTPNGIISPATCVYYTKW 244

BLAST of HG10001669 vs. TAIR 10
Match: AT5G23710.1 (DNA binding;DNA-directed RNA polymerases )

HSP 1 Score: 201.1 bits (510), Expect = 1.0e-51
Identity = 106/232 (45.69%), Postives = 143/232 (61.64%), Query Frame = 0

Query: 11  KRKRPDSAHGSGGALIEHEKVLHDLICSKQSMGIWIGDIRRETKYADSVVTKSLKSLQAR 70
           KRKRPD     G     HEK L DLI   Q  G  + +++RE     ++VT+ + SL+ +
Sbjct: 3   KRKRPDPKSSGGDLNEPHEKKLLDLIRINQGRGATMFELKREKTIPATIVTRLIASLRKK 62

Query: 71  GLIKLVEHYQNKGKKFYLSKDFEPSKELTGGNWYSDGKLDQEYINTLKGFCARMIQ--KL 130
            LIK V +  NKG K YL+ +FEP  ELTGG WY+DG LD   I  LK  C  +++  + 
Sbjct: 63  NLIKEVANMNNKGVKHYLAMEFEPCSELTGGEWYTDGALDLSKIEDLKAKCVMILERHRQ 122

Query: 131 KLATIEGIVDSFKKSGASKVDLSKEQIEEIVNALVLDNEVMEVKSTGSGEFDFIPVGKIC 190
           ++ T+E +   F K    +  LS +Q +EI+  L+LDN +MEVKS G  EF    +G++C
Sbjct: 123 RVVTLEVLCGYFVK----EEKLSVDQTKEILKNLILDNLIMEVKSNGMNEFASTRIGEVC 182

Query: 191 YKF-AKGGVKGEPKTGAMASIPCGVCPRISHCTPDGIISPRTCVYYTKWLDF 240
           Y+   K    GEP+ GA ASIPCGVCP I+ C+PDG+ISP TCVY+ KWLDF
Sbjct: 183 YRLTGKKFGNGEPRAGAFASIPCGVCPHIAICSPDGVISPTTCVYFQKWLDF 230

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149571.18.0e-12390.83DNA-directed RNA polymerase III subunit rpc6 isoform X1 [Momordica charantia] >X... [more]
XP_022970903.12.6e-12189.54DNA-directed RNA polymerase III subunit rpc6 [Cucurbita maxima][more]
KAG6604735.13.4e-12189.54DNA-directed RNA polymerase III subunit rpc6, partial [Cucurbita argyrosperma su... [more]
XP_022946967.13.4e-12189.54DNA-directed RNA polymerase III subunit rpc6 [Cucurbita moschata] >XP_023532566.... [more]
XP_038900531.16.8e-11488.66LOW QUALITY PROTEIN: DNA-directed RNA polymerase III subunit RPC6-like [Benincas... [more]
Match NameE-valueIdentityDescription
Q86JM31.0e-2428.75DNA-directed RNA polymerase III subunit rpc6 OS=Dictyostelium discoideum OX=4468... [more]
Q2T9S31.8e-2431.44DNA-directed RNA polymerase III subunit RPC6 OS=Bos taurus OX=9913 GN=POLR3F PE=... [more]
Q9H1D91.8e-2431.44DNA-directed RNA polymerase III subunit RPC6 OS=Homo sapiens OX=9606 GN=POLR3F P... [more]
Q921X62.3e-2431.44DNA-directed RNA polymerase III subunit RPC6 OS=Mus musculus OX=10090 GN=Polr3f ... [more]
Q9VD256.4e-2228.19Probable DNA-directed RNA polymerase III subunit RPC6 OS=Drosophila melanogaster... [more]
Match NameE-valueIdentityDescription
A0A6J1D8D13.9e-12390.83DNA-directed RNA polymerase III subunit rpc6 isoform X1 OS=Momordica charantia O... [more]
A0A6J1I5991.3e-12189.54DNA-directed RNA polymerase III subunit rpc6 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1G5531.6e-12189.54DNA-directed RNA polymerase III subunit rpc6 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A1S3BL061.4e-10478.60DNA-directed RNA polymerase III subunit rpc6-like OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A5D3D7H71.4e-10478.60DNA-directed RNA polymerase III subunit rpc6-like OS=Cucumis melo var. makuwa OX... [more]
Match NameE-valueIdentityDescription
AT5G23710.11.0e-5145.69DNA binding;DNA-directed RNA polymerases [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007832RNA polymerase Rpc34PFAMPF05158RNA_pol_Rpc34coord: 130..238
e-value: 1.3E-14
score: 54.4
coord: 27..127
e-value: 7.9E-25
score: 87.9
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 27..90
e-value: 1.6E-14
score: 55.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
IPR016049RNA polymerase Rpc34-likePANTHERPTHR12780RNA POLYMERASE III DNA DIRECTED , 39KD SUBUNIT-RELATEDcoord: 23..239
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 27..89

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10001669.1HG10001669.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006383 transcription by RNA polymerase III
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0008168 methyltransferase activity