HG10001591 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10001591
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptiontrans-cinnamate 4-monooxygenase
LocationChr09: 18446973 .. 18450525 (-)
RNA-Seq ExpressionHG10001591
SyntenyHG10001591
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCTCCTCCTCCTGGAGAAGACTCTCATCGGCCTCTTCCTCGCCGTCGTTCTCGCCATCGTCATTTCCAAGCTTCGCGGCAAGCGTTTCAAGCTTCCGCCAGGTCCCTTGCCGGTGCCGATCTTCGGAAACTGGCTCCAGGTCGGCGATGATTTGAACCACCGCAATCTCACAGATCTCGCCAAGAAATTCGGCGATATTTTCTTGCTTCGGATGGGACAGAGGAACCTCGTGGTGATTTCCTCGCCGGAATTAGCCAAGGAAGTGCTCCACACTCAGGGAGTCGAGTTCGGCTCTCGAACCAGAAACGTCGTCTTCGATATCTTCACCGGTAAAGGTCAGGATATGGTTTTCACCGTCTATGGCGAGCACTGGAGGAAAATGAGACGGATCATGACGGTTCCGTTTTTCACGAACAAAGTAGTCCAGCAATACCGTCAGGGATGGGAGGACGAAGCTCAAAGTGTGGTGGATGATGTGAAGAAGAATCCAGAATCGGCGACTGCTGGAGTCGTTCTCCGACGGCGGTTACAGCTTATGATGTACAATAACATGTACAGGATTATGTTCGATAGAAGATTCGAGAGTGAAGACGATCCTCTGTTTCACAAACTTAGGGCTCTGAACGGAGAGAGAAGCCGATTAGCGCAAAGCTTCGATTACAACTATGGCGATTTCATCCCGATCTTGAGGCCATTTTTGAGAGGATATCTCAAAATCTGCAAGGAAGTTAAGGAAACGAGGCTGAAACTCTTCAAGGATTACTTCGTCGAGGAGAGGAAGTGAGTTGAAAATCAACTGATCTGAATCGAATTCAATTTCCATCTGTTCTGTGTGTTAGTTGTTGAATAATTTACTGAAAATCTATGAAAAATTTTTAACAGGAAACTGGCGAATACAAAGAGCACGACGAACGAGGGATTGAAATGTGCGATTGATCATATATTGGATGCGCAACAGAAGGGAGAAATCAACGAAGACAATGTGCTCTACATTGTTGAGAATATCAATGTGGCAGGTAAATTCTTGAGTCTAATTTACTCCATTTTTCTTTGGGTTTGGTAGTTGCTCAACAAACTTTGGCTAGCGTGATAGATAAAGAATAATTAAACAAGTGGTTTAAATCTTTTAATAAATTTTTATAAATTATTCTATATTGGACTTTTCAAATTAAACATTTAGAAGAAATCATACACACTTGACTCAAACTCACAAAATTGGCCTTCTCATGGCTAAGGGTATTATCGTATTTTCATGCTTTTTAGAAAATATGAACAATGAATATGACTGCTTTCATTAATATTGACGGTTCATTTTGAACTTGGTTCAAAATGTTCATTTTAGTTATTGTATTTCTATATATTCAAAAAATATATTTGATTTTTGTAGTTTGAAAGAGTGATTATTTTGGTAATTTCTTAGACTTAAACGAATATTTTGAAAATAGACATTAATAGACTTCTATCAGTGATAGATTTTTTTCATTTCTATTACTGGTAAAATCTGATAGACTTCTATTAGCATTTATCACAACTATCTAAAATTTTGTTATTTTGTATAAATAGTTTTTCTTATTTTTTTATTTTTAAAAATTTTCCTAAAACCACCTGAAGTAAAATTAAGATTAATGCTTAAAGTAATATTTAGCAAAAGAAAAAAATAATAATAATAAAATAAAAGACTTAGTAGGTCACGGGACAACGATATCCCACACCCTTACTTGTGATTATCTTCAAATAAAATAAGATCGATTGAGCATATGGGCTTTTTTTTATATTAAAAAATTATTTATTTATTTATTTATGATCAGCTTTTTAAGAGATAGACAACAACCCATTTCCATTTTTGTAAGTATTAGAACCATTTCCATTTTTTAAATTTTTTAATGAACACAGTAAAGAAAACCAAGTGTGACCAATAATTACTACTGGCTTTTTATGGAAAGAAAAATAGACGAAAGAGTCAAGTATAGACACTTGTCCTGCCATGGAAAGCAAACAACTCCTTTCCTATTTTACAAAAAAAAATTCTGCATAATAGTTAAAGGGAAAATTTTTAAAAATAAAAAAATAAGAAAATTATTTACACAAAATAGTAAAATTTTTAGATAGTTGTAATAGACGCTGATAGAAGTCTATCAAAGTCTATCAGTGATAGAAATGATAAAAGTCTATTAATGCTGCTAAAAGTCTTATCAATGTCTATTAATCTTTTTTTTGAGAAATTTTCACAAATAAAAAAAATGTCAAACTATTTACATAAAATAGCAAAAAAAAAAATGCTGATAGACATTGATAGACTTCTATCAGCATCTATCGGTGATAGACTTCTATCATTTCTATTACTGATAGATCCTGATAGACTTCTATCCGCGTCTATCATAACTATTTAAAAATTTTGCTATTTTGTGTAAATAGTTTGATTTATTTTTCTATTTTTAAAACTTCTCTTTTTTTTTGTTATTTTCTGTAAATAGTTTGATATTTTTTCTATCTATGAAAATTTCTCTAGTTAAAGTTCTCGATTTTTTATTTTTAATTTTTAATTTTTGGAAATTAAGTCAAATCCTACTTTTGGTTTTTGAAAATTAAGTCAAACACTCATTCTTACGTTTTGGTTTGTTATTTACTTTTTATTAATATTTGTAAAAATTAAACCACATTTTAAAAACTAAAAAAAAAAAAAAGATTAGTTCTTAGAAACTTGTCTTTGTTGGTTGAAATTTGGCTATGTATTAACTCTTTTACCTAAGAAATATGAAACTATGGTAGGAAATTGAGAGGAAACAGACCTAACAAAGTCCAAAATAGTAACTATGATAGCTACCCACAACCACAACATTATTTGAAATTTGGTTGCATTTTAAATCTAAAATATATGAATGGTGGTGACAAAAACAAAGAACAATGATAGTTATTGATTATGTGTTTATGGTTTATATTGTTGAACAGCAATTGAAACAACATTATGGTCAGTTGAATGGGGAATTGCTGAGCTAGTGAACCACCCAGAAATCCAAAGAAAGTTAAGGAATGAACTCGACACTGTCCTTGGACCTGGTGTTCCAATCACCGAGCCAGACACTCACAAGCTCCCTTACCTTCAAGCCGTGGTCAAGGAGACTCTCCGCCTTCGCATGGCCATCCCTTTACTCGTCCCCCACATGAACCTCCACGACGCCAAGCTCGGTGGCTATGACATTCCGGCCGAGAGCAAGATCCTAGTCAATGCCTGGTGGCTTGCCAACAACCCAGCCAACTGGAAGAACCCCGAAGAATTCCGCCCCGAGAGGTTCTTGGAGGAGGAATCCAAGGTTGAAGCCAATGGCAATGATTTCAGATACTTGCCTTTCGGCGTCGGGCGAAGAAGCTGCCCTGGAATCATCTTGGCTTTGCCAATTCTTGGCATTACAATTGGGCGTTTGGTTCAGAACTTTGAGCTGCTTCCTCCTCCTGGACAGTCTAAGTTGGATACTTCAGAGAAAGGTGGACAATTTAGCTTGCATATTTTGAAGCACTCTACCATTGTTGCTAAGCCAAGAGCGTTCTAA

mRNA sequence

ATGGATCTCCTCCTCCTGGAGAAGACTCTCATCGGCCTCTTCCTCGCCGTCGTTCTCGCCATCGTCATTTCCAAGCTTCGCGGCAAGCGTTTCAAGCTTCCGCCAGGTCCCTTGCCGGTGCCGATCTTCGGAAACTGGCTCCAGGTCGGCGATGATTTGAACCACCGCAATCTCACAGATCTCGCCAAGAAATTCGGCGATATTTTCTTGCTTCGGATGGGACAGAGGAACCTCGTGGTGATTTCCTCGCCGGAATTAGCCAAGGAAGTGCTCCACACTCAGGGAGTCGAGTTCGGCTCTCGAACCAGAAACGTCGTCTTCGATATCTTCACCGGTAAAGGTCAGGATATGGTTTTCACCGTCTATGGCGAGCACTGGAGGAAAATGAGACGGATCATGACGGTTCCGTTTTTCACGAACAAAGTAGTCCAGCAATACCGTCAGGGATGGGAGGACGAAGCTCAAAGTGTGGTGGATGATGTGAAGAAGAATCCAGAATCGGCGACTGCTGGAGTCGTTCTCCGACGGCGGTTACAGCTTATGATGTACAATAACATGTACAGGATTATGTTCGATAGAAGATTCGAGAGTGAAGACGATCCTCTGTTTCACAAACTTAGGGCTCTGAACGGAGAGAGAAGCCGATTAGCGCAAAGCTTCGATTACAACTATGGCGATTTCATCCCGATCTTGAGGCCATTTTTGAGAGGATATCTCAAAATCTGCAAGGAAGTTAAGGAAACGAGGCTGAAACTCTTCAAGGATTACTTCGTCGAGGAGAGGAAGAAACTGGCGAATACAAAGAGCACGACGAACGAGGGATTGAAATGTGCGATTGATCATATATTGGATGCGCAACAGAAGGGAGAAATCAACGAAGACAATGTGCTCTACATTGTTGAGAATATCAATGTGGCAGCAATTGAAACAACATTATGGTCAGTTGAATGGGGAATTGCTGAGCTAGTGAACCACCCAGAAATCCAAAGAAAGTTAAGGAATGAACTCGACACTGTCCTTGGACCTGGTGTTCCAATCACCGAGCCAGACACTCACAAGCTCCCTTACCTTCAAGCCGTGGTCAAGGAGACTCTCCGCCTTCGCATGGCCATCCCTTTACTCGTCCCCCACATGAACCTCCACGACGCCAAGCTCGGTGGCTATGACATTCCGGCCGAGAGCAAGATCCTAGTCAATGCCTGGTGGCTTGCCAACAACCCAGCCAACTGGAAGAACCCCGAAGAATTCCGCCCCGAGAGGTTCTTGGAGGAGGAATCCAAGGTTGAAGCCAATGGCAATGATTTCAGATACTTGCCTTTCGGCGTCGGGCGAAGAAGCTGCCCTGGAATCATCTTGGCTTTGCCAATTCTTGGCATTACAATTGGGCGTTTGGTTCAGAACTTTGAGCTGCTTCCTCCTCCTGGACAGTCTAAGTTGGATACTTCAGAGAAAGGTGGACAATTTAGCTTGCATATTTTGAAGCACTCTACCATTGTTGCTAAGCCAAGAGCGTTCTAA

Coding sequence (CDS)

ATGGATCTCCTCCTCCTGGAGAAGACTCTCATCGGCCTCTTCCTCGCCGTCGTTCTCGCCATCGTCATTTCCAAGCTTCGCGGCAAGCGTTTCAAGCTTCCGCCAGGTCCCTTGCCGGTGCCGATCTTCGGAAACTGGCTCCAGGTCGGCGATGATTTGAACCACCGCAATCTCACAGATCTCGCCAAGAAATTCGGCGATATTTTCTTGCTTCGGATGGGACAGAGGAACCTCGTGGTGATTTCCTCGCCGGAATTAGCCAAGGAAGTGCTCCACACTCAGGGAGTCGAGTTCGGCTCTCGAACCAGAAACGTCGTCTTCGATATCTTCACCGGTAAAGGTCAGGATATGGTTTTCACCGTCTATGGCGAGCACTGGAGGAAAATGAGACGGATCATGACGGTTCCGTTTTTCACGAACAAAGTAGTCCAGCAATACCGTCAGGGATGGGAGGACGAAGCTCAAAGTGTGGTGGATGATGTGAAGAAGAATCCAGAATCGGCGACTGCTGGAGTCGTTCTCCGACGGCGGTTACAGCTTATGATGTACAATAACATGTACAGGATTATGTTCGATAGAAGATTCGAGAGTGAAGACGATCCTCTGTTTCACAAACTTAGGGCTCTGAACGGAGAGAGAAGCCGATTAGCGCAAAGCTTCGATTACAACTATGGCGATTTCATCCCGATCTTGAGGCCATTTTTGAGAGGATATCTCAAAATCTGCAAGGAAGTTAAGGAAACGAGGCTGAAACTCTTCAAGGATTACTTCGTCGAGGAGAGGAAGAAACTGGCGAATACAAAGAGCACGACGAACGAGGGATTGAAATGTGCGATTGATCATATATTGGATGCGCAACAGAAGGGAGAAATCAACGAAGACAATGTGCTCTACATTGTTGAGAATATCAATGTGGCAGCAATTGAAACAACATTATGGTCAGTTGAATGGGGAATTGCTGAGCTAGTGAACCACCCAGAAATCCAAAGAAAGTTAAGGAATGAACTCGACACTGTCCTTGGACCTGGTGTTCCAATCACCGAGCCAGACACTCACAAGCTCCCTTACCTTCAAGCCGTGGTCAAGGAGACTCTCCGCCTTCGCATGGCCATCCCTTTACTCGTCCCCCACATGAACCTCCACGACGCCAAGCTCGGTGGCTATGACATTCCGGCCGAGAGCAAGATCCTAGTCAATGCCTGGTGGCTTGCCAACAACCCAGCCAACTGGAAGAACCCCGAAGAATTCCGCCCCGAGAGGTTCTTGGAGGAGGAATCCAAGGTTGAAGCCAATGGCAATGATTTCAGATACTTGCCTTTCGGCGTCGGGCGAAGAAGCTGCCCTGGAATCATCTTGGCTTTGCCAATTCTTGGCATTACAATTGGGCGTTTGGTTCAGAACTTTGAGCTGCTTCCTCCTCCTGGACAGTCTAAGTTGGATACTTCAGAGAAAGGTGGACAATTTAGCTTGCATATTTTGAAGCACTCTACCATTGTTGCTAAGCCAAGAGCGTTCTAA

Protein sequence

MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTDLAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQLMMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLKICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIVENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAVVKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPERFLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLDTSEKGGQFSLHILKHSTIVAKPRAF
Homology
BLAST of HG10001591 vs. NCBI nr
Match: XP_008456253.1 (PREDICTED: trans-cinnamate 4-monooxygenase [Cucumis melo] >KAA0033521.1 trans-cinnamate 4-monooxygenase [Cucumis melo var. makuwa] >TYJ95667.1 trans-cinnamate 4-monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 1010.0 bits (2610), Expect = 7.3e-291
Identity = 495/505 (98.02%), Postives = 501/505 (99.21%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTL+GLFLAVVLAI ISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1   MDLLLLEKTLLGLFLAVVLAIAISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
           LAKKFGDIFLLRMGQRNLVV+SSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWE EAQSVVDDVKKNPESAT G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEFEAQSVVDDVKKNPESATNGIVLRRRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSF+YNYGDFIPILRPFLRGYLK
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV
Sbjct: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           +KETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD
Sbjct: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           TSEKGGQFSLHILKHSTIVAKPR F
Sbjct: 481 TSEKGGQFSLHILKHSTIVAKPRVF 505

BLAST of HG10001591 vs. NCBI nr
Match: XP_038901578.1 (trans-cinnamate 4-monooxygenase [Benincasa hispida])

HSP 1 Score: 1007.7 bits (2604), Expect = 3.6e-290
Identity = 496/505 (98.22%), Postives = 500/505 (99.01%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTLIGLFLAVVLAI ISK RGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1   MDLLLLEKTLIGLFLAVVLAIAISKFRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
           LAKKFGDIFLLRMGQRNLVV+SSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWE EAQSVVDDVKKNPESAT GVVLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEYEAQSVVDDVKKNPESATTGVVLRRRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSF+YNYGDFIPILRPFLRGYLK
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV
Sbjct: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNH EIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHQEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           VKETLRLRMAIPLLVPHMNLH+AKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHEAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD
Sbjct: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           TSEKGGQFSLHILKHSTIVAKPRAF
Sbjct: 481 TSEKGGQFSLHILKHSTIVAKPRAF 505

BLAST of HG10001591 vs. NCBI nr
Match: KGN46771.2 (hypothetical protein Csa_020744 [Cucumis sativus])

HSP 1 Score: 998.8 bits (2581), Expect = 1.7e-287
Identity = 489/505 (96.83%), Postives = 496/505 (98.22%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTL+GLFL+VVLAI ISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1   MDLLLLEKTLLGLFLSVVLAIAISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
           LAKKFGDIFLLRMGQRNLVV+SSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWE EAQSVVDDVKKNPESAT G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEFEAQSVVDDVKKNPESATTGIVLRRRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSF+YNYGDFIPILRPFLRGYLK
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV
Sbjct: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDT KLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTQKLPYLQAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           VKETLRLRM IPLLVPHMNLHDAKL GYDIPAESKILVNAWWLANNPANWKNPEEFRPER
Sbjct: 361 VKETLRLRMTIPLLVPHMNLHDAKLAGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           FLEEESKVEANGNDF+YLPFG GRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD
Sbjct: 421 FLEEESKVEANGNDFKYLPFGAGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           TSEKGGQFSLHILKHSTIV KPR F
Sbjct: 481 TSEKGGQFSLHILKHSTIVVKPRVF 505

BLAST of HG10001591 vs. NCBI nr
Match: NP_001292707.1 (trans-cinnamate 4-monooxygenase [Cucumis sativus] >CAK95273.1 cinnamate-4-hydroxylase [Cucumis sativus])

HSP 1 Score: 997.7 bits (2578), Expect = 3.7e-287
Identity = 489/505 (96.83%), Postives = 496/505 (98.22%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTL+GLFL+VVLAI ISKLRGKRFK PPGPLPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1   MDLLLLEKTLLGLFLSVVLAIAISKLRGKRFKPPPGPLPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
           LAKKFGDIFLLRMGQRNLVV+SSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWE EAQSVVDDVKKNPESAT G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEFEAQSVVDDVKKNPESATTGIVLRRRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSF+YNYGDFIPILRPFLRGYLK
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV
Sbjct: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDT KLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTQKLPYLQAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           VKETLRLRMAIPLLVPHMNLHDAKL GYDIPAESKILVNAWWLANNPANWKNPEEFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHDAKLAGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           FLEEESKVEANGNDF+YLPFG GRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD
Sbjct: 421 FLEEESKVEANGNDFKYLPFGAGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           TSEKGGQFSLHILKHSTIV KPR F
Sbjct: 481 TSEKGGQFSLHILKHSTIVVKPRVF 505

BLAST of HG10001591 vs. NCBI nr
Match: XP_023007159.1 (trans-cinnamate 4-monooxygenase [Cucurbita maxima])

HSP 1 Score: 988.0 bits (2553), Expect = 3.0e-284
Identity = 484/505 (95.84%), Postives = 494/505 (97.82%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTL+GLF+AVVLAI ISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1   MDLLLLEKTLVGLFVAVVLAIAISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
           LAKKFGDIFLLRMGQRNLVV+SSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYR GWE EA SVV+DVKKNPESAT G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEYEAASVVEDVKKNPESATTGIVLRRRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNMYRIMFDRRFESEDDPLF KLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFQKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           ICKEVKETRLKLFKDYFVEERKKLANTKST+NEGLKCAIDHILDAQQKGEINEDNVLYIV
Sbjct: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTSNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHPEIQRKLRNELDTVLG  VPITEPDTHKLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQRKLRNELDTVLGADVPITEPDTHKLPYLQAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           +KETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWK+PE FRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKDPEVFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRL+QNFELLPPPG SKLD
Sbjct: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLIQNFELLPPPGHSKLD 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           TSEKGGQFSLHILKHSTIVAKPR F
Sbjct: 481 TSEKGGQFSLHILKHSTIVAKPRVF 505

BLAST of HG10001591 vs. ExPASy Swiss-Prot
Match: Q43054 (Trans-cinnamate 4-monooxygenase OS=Populus kitakamiensis OX=34292 GN=CYP73A16 PE=2 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 3.6e-277
Identity = 460/505 (91.09%), Postives = 494/505 (97.82%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTL+G F+AV++AI++SKLRGKRFKLPPGPLPVP+FGNWLQVGDDLNHRNLTD
Sbjct: 1   MDLLLLEKTLLGSFVAVLVAILVSKLRGKRFKLPPGPLPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
           LAKKFGDIFLLRMGQRNLVV+SSP+L+KEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPDLSKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYR GWE+EA  VV+DVKKNPE+AT G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEEEAAQVVEDVKKNPEAATNGIVLRRRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNMYRIMFDRRFESEDDPLF+KL+ALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFNKLKALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           IC+EVKE RL+LFKDYFV+ERKKLA+TK+ +NEGLKCAIDHILDAQ+KGEINEDNVLYIV
Sbjct: 241 ICQEVKERRLQLFKDYFVDERKKLASTKNMSNEGLKCAIDHILDAQKKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHPEIQ+KLR+ELDT+LGPG  ITEPDT+KLPYL AV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKLRHELDTLLGPGHQITEPDTYKLPYLNAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           VKETLRLRMAIPLLVPHMNLHDAKLGG+DIPAESKILVNAWWLANNPA+WKNPEEFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPAHWKNPEEFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           FLEE +KVEANGNDFRYLPFGVGRRSCPGIILALPILGIT+GRLVQNFELLPPPGQSK+D
Sbjct: 421 FLEEGAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKID 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           TSEKGGQFSLHILKHSTIVAKPR+F
Sbjct: 481 TSEKGGQFSLHILKHSTIVAKPRSF 505

BLAST of HG10001591 vs. ExPASy Swiss-Prot
Match: O24312 (Trans-cinnamate 4-monooxygenase OS=Populus tremuloides OX=3693 GN=CYP73A13 PE=2 SV=1)

HSP 1 Score: 951.0 bits (2457), Expect = 5.2e-276
Identity = 458/505 (90.69%), Postives = 492/505 (97.43%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTL+G F+A+++AI++SKLRGKRFKLPPGPLPVP+FGNWLQVGDDLNHRNLTD
Sbjct: 1   MDLLLLEKTLLGSFVAILVAILVSKLRGKRFKLPPGPLPVPVFGNWLQVGDDLNHRNLTD 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
           LAKKFGDI LLRMGQRNLVV+SSPEL+KEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  LAKKFGDILLLRMGQRNLVVVSSPELSKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYR GWE+EA  VV+DVKKNP +AT G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEEEAAQVVEDVKKNPGAATHGIVLRRRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNMYRIMFDRRFESE+DPLF+KL+ALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK
Sbjct: 181 MMYNNMYRIMFDRRFESEEDPLFNKLKALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           IC+EVKE RL+LFKDYFV+ERKKLA+TK+  NEGLKCAIDHILDAQ+KGEINEDNVLYIV
Sbjct: 241 ICQEVKERRLQLFKDYFVDERKKLASTKNMCNEGLKCAIDHILDAQKKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHPEIQ+KLR+ELDT+LGPG  ITEPDT+KLPYL AV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKLRHELDTLLGPGHQITEPDTYKLPYLNAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           VKETLRLRMAIPLLVPHMNLHDAKLGG+DIPAESKILVNAWWLANNPA+WKNPEEFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGFDIPAESKILVNAWWLANNPAHWKNPEEFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           FLEEE+KVEANGNDFRYLPFGVGRRSCPGIILALPILGIT+GRLVQNFELLPPPGQSK+D
Sbjct: 421 FLEEEAKVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKID 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           TSEKGGQFSLHILKHSTIVAKPR+F
Sbjct: 481 TSEKGGQFSLHILKHSTIVAKPRSF 505

BLAST of HG10001591 vs. ExPASy Swiss-Prot
Match: Q96423 (Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza echinata OX=46348 GN=CYP73A14 PE=2 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 1.3e-274
Identity = 456/505 (90.30%), Postives = 486/505 (96.24%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTL+GLF+A + AI ISKLRG+RFKLPPGP+PVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1   MDLLLLEKTLLGLFIAAITAIAISKLRGRRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
           LAK+FGDIFLLRMGQRNLVV+SSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  LAKRFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYR GWE EA SVVDDV++NP++A  G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRFGWESEAASVVDDVRRNPDAAAGGIVLRRRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNMYRIMFDRRFESE+DPLF KL+ALNGERSRLAQSF+YNYGDFIPILRPFL+GYLK
Sbjct: 181 MMYNNMYRIMFDRRFESEEDPLFVKLKALNGERSRLAQSFEYNYGDFIPILRPFLKGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           ICKEVKE RLKLFKDYFV+ER KL +TKST+NEGLKCAIDHILDAQ+KGEINEDNVLYIV
Sbjct: 241 ICKEVKERRLKLFKDYFVDERMKLESTKSTSNEGLKCAIDHILDAQKKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHPEIQ+K+R+E+D VLGPG  +TEPD  KLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQKKVRDEIDRVLGPGHQVTEPDMQKLPYLQAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           +KETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWK PEEFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKRPEEFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           FLEEES VEANGNDFRYLPFGVGRRSCPGIILALPILGIT+GRLVQNFELLPPPGQSKLD
Sbjct: 421 FLEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKLD 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           T+EKGGQFSLHILKHSTIVAKPR+F
Sbjct: 481 TAEKGGQFSLHILKHSTIVAKPRSF 505

BLAST of HG10001591 vs. ExPASy Swiss-Prot
Match: P37115 (Trans-cinnamate 4-monooxygenase OS=Vigna radiata var. radiata OX=3916 GN=CYP73A2 PE=1 SV=1)

HSP 1 Score: 937.9 bits (2423), Expect = 4.6e-272
Identity = 450/505 (89.11%), Postives = 486/505 (96.24%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTL+GLFLA V+AIV+SKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLT 
Sbjct: 1   MDLLLLEKTLLGLFLAAVVAIVVSKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTQ 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
           LAK+FGDIFLLRMGQRNLVV+SSP+LAKEVLHTQGVEFGSRTRNVVFDIFTG+GQDMVFT
Sbjct: 61  LAKRFGDIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGEGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYR GWE EA +VVDDV+KNP++A +G+V+RRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRHGWEAEAAAVVDDVRKNPDAAVSGLVIRRRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNMYRIMFDRRFESE+DPLF +L+ALNGERSRLAQSF+YNYGDFIPILRPFL+GYLK
Sbjct: 181 MMYNNMYRIMFDRRFESEEDPLFQRLKALNGERSRLAQSFEYNYGDFIPILRPFLKGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           ICKEVKETRLKLFKDYFV+ERK + +TKST NEGLKCAIDHILDA++KGEINEDNVLYIV
Sbjct: 241 ICKEVKETRLKLFKDYFVDERKNIGSTKSTNNEGLKCAIDHILDAEKKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHPEIQ+K+R+E+D VLG G  +TEPD  KLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQQKVRDEIDRVLGVGHQVTEPDIQKLPYLQAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPA+WK PEEFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPAHWKKPEEFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           F EEES VEANGNDFRYLPFGVGRRSCPGIILALPILGIT+GRLVQNFELLPPPGQS++D
Sbjct: 421 FFEEESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSQID 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           TSEKGGQFSLHILKHST+VAKPR+F
Sbjct: 481 TSEKGGQFSLHILKHSTVVAKPRSF 505

BLAST of HG10001591 vs. ExPASy Swiss-Prot
Match: F1B283 (Trans-cinnamate 4-monooxygenase C4H2 OS=Petunia hybrida OX=4102 GN=C4H2 PE=2 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 1.0e-271
Identity = 449/505 (88.91%), Postives = 483/505 (95.64%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTLIGLF+A+++A ++SKLR KRFKLPPGP+PVP+FGNWLQVGDDLNHRNLT+
Sbjct: 1   MDLLLLEKTLIGLFIAIIIATIVSKLRSKRFKLPPGPIPVPVFGNWLQVGDDLNHRNLTE 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
            AKKFGD+FLLRMGQRNLVV+SSP+LAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  YAKKFGDLFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYR GWE E +SVV+DVKK  ES T G+VLR+RLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRGGWEYEVESVVEDVKKMKESNTNGIVLRKRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNM+RIMFDRRFESEDDPLF KL+ALNGERSRLAQSF+YNYGDFIPILRPFLRGYLK
Sbjct: 181 MMYNNMFRIMFDRRFESEDDPLFVKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           ICKEVKE RLKLFKDYFV+ERKKLANTKS  +  LKCAIDHIL+AQQKGEINEDNVLYIV
Sbjct: 241 ICKEVKEKRLKLFKDYFVDERKKLANTKSMDSNALKCAIDHILEAQQKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHP IQ+KLR+E+DTVLGPGV +TEPDTHKLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPHIQKKLRDEIDTVLGPGVQVTEPDTHKLPYLQAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           +KETLRLRMAIPLLVPHMNLH+AKLGGYDIPAESKILVNAWWLANNPA+WKNPEEFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHEAKLGGYDIPAESKILVNAWWLANNPAHWKNPEEFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           F EEE  VEANGNDFRYLPFGVGRRSCPGIILALPILGIT+GRLVQNFELLPPPGQSK+D
Sbjct: 421 FFEEEKHVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKID 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           T+EKGGQFSLHILKHSTIV KPR+F
Sbjct: 481 TTEKGGQFSLHILKHSTIVLKPRSF 505

BLAST of HG10001591 vs. ExPASy TrEMBL
Match: A0A5D3B966 (Trans-cinnamate 4-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold104G00990 PE=3 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 3.5e-291
Identity = 495/505 (98.02%), Postives = 501/505 (99.21%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTL+GLFLAVVLAI ISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1   MDLLLLEKTLLGLFLAVVLAIAISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
           LAKKFGDIFLLRMGQRNLVV+SSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWE EAQSVVDDVKKNPESAT G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEFEAQSVVDDVKKNPESATNGIVLRRRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSF+YNYGDFIPILRPFLRGYLK
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV
Sbjct: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           +KETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD
Sbjct: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           TSEKGGQFSLHILKHSTIVAKPR F
Sbjct: 481 TSEKGGQFSLHILKHSTIVAKPRVF 505

BLAST of HG10001591 vs. ExPASy TrEMBL
Match: A0A1S3C2S5 (trans-cinnamate 4-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103496249 PE=3 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 3.5e-291
Identity = 495/505 (98.02%), Postives = 501/505 (99.21%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTL+GLFLAVVLAI ISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1   MDLLLLEKTLLGLFLAVVLAIAISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
           LAKKFGDIFLLRMGQRNLVV+SSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWE EAQSVVDDVKKNPESAT G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEFEAQSVVDDVKKNPESATNGIVLRRRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSF+YNYGDFIPILRPFLRGYLK
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV
Sbjct: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           +KETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD
Sbjct: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           TSEKGGQFSLHILKHSTIVAKPR F
Sbjct: 481 TSEKGGQFSLHILKHSTIVAKPRVF 505

BLAST of HG10001591 vs. ExPASy TrEMBL
Match: Q17UC0 (Cinnamate-4-hydroxylase OS=Cucumis sativus OX=3659 GN=c4H PE=2 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 1.8e-287
Identity = 489/505 (96.83%), Postives = 496/505 (98.22%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTL+GLFL+VVLAI ISKLRGKRFK PPGPLPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1   MDLLLLEKTLLGLFLSVVLAIAISKLRGKRFKPPPGPLPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
           LAKKFGDIFLLRMGQRNLVV+SSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWE EAQSVVDDVKKNPESAT G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEFEAQSVVDDVKKNPESATTGIVLRRRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSF+YNYGDFIPILRPFLRGYLK
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV
Sbjct: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDT KLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTQKLPYLQAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           VKETLRLRMAIPLLVPHMNLHDAKL GYDIPAESKILVNAWWLANNPANWKNPEEFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHDAKLAGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           FLEEESKVEANGNDF+YLPFG GRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD
Sbjct: 421 FLEEESKVEANGNDFKYLPFGAGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           TSEKGGQFSLHILKHSTIV KPR F
Sbjct: 481 TSEKGGQFSLHILKHSTIVVKPRVF 505

BLAST of HG10001591 vs. ExPASy TrEMBL
Match: A0A6J1L470 (trans-cinnamate 4-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499735 PE=3 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 1.4e-284
Identity = 484/505 (95.84%), Postives = 494/505 (97.82%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTL+GLF+AVVLAI ISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1   MDLLLLEKTLVGLFVAVVLAIAISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
           LAKKFGDIFLLRMGQRNLVV+SSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYR GWE EA SVV+DVKKNPESAT G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEYEAASVVEDVKKNPESATTGIVLRRRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNMYRIMFDRRFESEDDPLF KLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFQKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           ICKEVKETRLKLFKDYFVEERKKLANTKST+NEGLKCAIDHILDAQQKGEINEDNVLYIV
Sbjct: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTSNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHPEIQRKLRNELDTVLG  VPITEPDTHKLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQRKLRNELDTVLGADVPITEPDTHKLPYLQAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           +KETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWK+PE FRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKDPEVFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRL+QNFELLPPPG SKLD
Sbjct: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLIQNFELLPPPGHSKLD 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           TSEKGGQFSLHILKHSTIVAKPR F
Sbjct: 481 TSEKGGQFSLHILKHSTIVAKPRVF 505

BLAST of HG10001591 vs. ExPASy TrEMBL
Match: A0A6J1G748 (trans-cinnamate 4-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111451474 PE=3 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 5.4e-284
Identity = 483/505 (95.64%), Postives = 495/505 (98.02%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEKTL+GLF+AVVLAI ISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD
Sbjct: 1   MDLLLLEKTLVGLFVAVVLAIAISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
           LAKKFGDIFLLRMGQRNLVV+SSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  LAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQYR GWE EA SVV+DVKKNPESAT+G+VLRRRLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEYEAASVVEDVKKNPESATSGIVLRRRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNMYRIMFDRRFESEDDPLF KLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK
Sbjct: 181 MMYNNMYRIMFDRRFESEDDPLFQKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           ICKEVKETRLKLFKDYFVEERKKLANTKST+NEGLKCAIDHILDAQQKGEINEDNVLYIV
Sbjct: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTSNEGLKCAIDHILDAQQKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHPEIQRKLRNELDTVLG  VPITEPDT+KLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQRKLRNELDTVLGADVPITEPDTYKLPYLQAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           +KETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWK+PE FRPER
Sbjct: 361 IKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKDPEVFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRL+QNFELLPPPG SKLD
Sbjct: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLIQNFELLPPPGHSKLD 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPRAF 506
           TSEKGGQFSLHILKHSTIVAKPR F
Sbjct: 481 TSEKGGQFSLHILKHSTIVAKPRVF 505

BLAST of HG10001591 vs. TAIR 10
Match: AT2G30490.1 (cinnamate-4-hydroxylase )

HSP 1 Score: 909.1 bits (2348), Expect = 1.6e-264
Identity = 438/503 (87.08%), Postives = 475/503 (94.43%), Query Frame = 0

Query: 1   MDLLLLEKTLIGLFLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTD 60
           MDLLLLEK+LI +F+AV+LA VISKLRGK+ KLPPGP+P+PIFGNWLQVGDDLNHRNL D
Sbjct: 1   MDLLLLEKSLIAVFVAVILATVISKLRGKKLKLPPGPIPIPIFGNWLQVGDDLNHRNLVD 60

Query: 61  LAKKFGDIFLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120
            AKKFGD+FLLRMGQRNLVV+SSP+L KEVL TQGVEFGSRTRNVVFDIFTGKGQDMVFT
Sbjct: 61  YAKKFGDLFLLRMGQRNLVVVSSPDLTKEVLLTQGVEFGSRTRNVVFDIFTGKGQDMVFT 120

Query: 121 VYGEHWRKMRRIMTVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQL 180
           VYGEHWRKMRRIMTVPFFTNKVVQQ R+GWE EA SVV+DVKKNP+SAT G+VLR+RLQL
Sbjct: 121 VYGEHWRKMRRIMTVPFFTNKVVQQNREGWEFEAASVVEDVKKNPDSATKGIVLRKRLQL 180

Query: 181 MMYNNMYRIMFDRRFESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLK 240
           MMYNNM+RIMFDRRFESEDDPLF +L+ALNGERSRLAQSF+YNYGDFIPILRPFLRGYLK
Sbjct: 181 MMYNNMFRIMFDRRFESEDDPLFLRLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK 240

Query: 241 ICKEVKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIV 300
           IC++VK+ R+ LFK YFV+ERK++A++K T +EGLKCAIDHIL+A+QKGEINEDNVLYIV
Sbjct: 241 ICQDVKDRRIALFKKYFVDERKQIASSKPTGSEGLKCAIDHILEAEQKGEINEDNVLYIV 300

Query: 301 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAV 360
           ENINVAAIETTLWS+EWGIAELVNHPEIQ KLRNELDTVLGPGV +TEPD HKLPYLQAV
Sbjct: 301 ENINVAAIETTLWSIEWGIAELVNHPEIQSKLRNELDTVLGPGVQVTEPDLHKLPYLQAV 360

Query: 361 VKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPER 420
           VKETLRLRMAIPLLVPHMNLHDAKL GYDIPAESKILVNAWWLANNP +WK PEEFRPER
Sbjct: 361 VKETLRLRMAIPLLVPHMNLHDAKLAGYDIPAESKILVNAWWLANNPNSWKKPEEFRPER 420

Query: 421 FLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLD 480
           F EEES VEANGNDFRY+PFGVGRRSCPGIILALPILGITIGR+VQNFELLPPPGQSK+D
Sbjct: 421 FFEEESHVEANGNDFRYVPFGVGRRSCPGIILALPILGITIGRMVQNFELLPPPGQSKVD 480

Query: 481 TSEKGGQFSLHILKHSTIVAKPR 504
           TSEKGGQFSLHIL HS IV KPR
Sbjct: 481 TSEKGGQFSLHILNHSIIVMKPR 503

BLAST of HG10001591 vs. TAIR 10
Match: AT2G40890.1 (cytochrome P450, family 98, subfamily A, polypeptide 3 )

HSP 1 Score: 285.8 bits (730), Expect = 6.7e-77
Identity = 174/481 (36.17%), Postives = 271/481 (56.34%), Query Frame = 0

Query: 15  LAVVLAIVISKL-RGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTDLAKKFGDIFLLRM 74
           +A + A+V  KL +  R+K PPGP P PI GN   +   +  R   + A+ +G I  + +
Sbjct: 8   VATIAAVVSYKLIQRLRYKFPPGPSPKPIVGNLYDI-KPVRFRCYYEWAQSYGPIISVWI 67

Query: 75  GQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIM 134
           G    VV+SS ELAKEVL     +   R RN   + F+  GQD+++  YG H+ K+R++ 
Sbjct: 68  GSILNVVVSSAELAKEVLKEHDQKLADRHRNRSTEAFSRNGQDLIWADYGPHYVKVRKVC 127

Query: 135 TVPFFTNKVVQQYRQGWEDEAQSVVDDVKKN---PESATAGVVLRRRLQLMMYNNMYRIM 194
           T+  FT K ++  R   EDE  ++V+ V ++   PE+   G+ LR+ L  + +NN+ R+ 
Sbjct: 128 TLELFTPKRLESLRPIREDEVTAMVESVFRDCNLPENRAKGLQLRKYLGAVAFNNITRLA 187

Query: 195 FDRRF---ESEDDPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPFLRGYLKICKEVKE 254
           F +RF   E   D    + +A+     +L  S   +  + IP LR       K   E   
Sbjct: 188 FGKRFMNAEGVVDEQGLEFKAIVSNGLKLGASL--SIAEHIPWLRWMFPADEKAFAEHGA 247

Query: 255 TRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIVENINVAA 314
            R +L +   + E   LA  KS+  +  +  +D +L  + + +++ED ++ ++ ++  A 
Sbjct: 248 RRDRLTR--AIMEEHTLARQKSSGAK--QHFVDALLTLKDQYDLSEDTIIGLLWDMITAG 307

Query: 315 IETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAVVKETLRL 374
           ++TT  + EW +AE++ +P +Q+K++ E D V+G    +TE D  +LPYLQ VVKE+ RL
Sbjct: 308 MDTTAITAEWAMAEMIKNPRVQQKVQEEFDRVVGLDRILTEADFSRLPYLQCVVKESFRL 367

Query: 375 RMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPERFLEEESK 434
               PL++PH +  D K+GGYDIP  S + VN W +A +PA WKNP EFRPERFLEE+  
Sbjct: 368 HPPTPLMLPHRSNADVKIGGYDIPKGSNVHVNVWAVARDPAVWKNPFEFRPERFLEED-- 427

Query: 435 VEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPG--QSKLDTSEKG 487
           V+  G+DFR LPFG GRR CPG  L + ++   +  L+ +F   PP G    ++D SE  
Sbjct: 428 VDMKGHDFRLLPFGAGRRVCPGAQLGINLVTSMMSHLLHHFVWTPPQGTKPEEIDMSENP 479

BLAST of HG10001591 vs. TAIR 10
Match: AT4G22690.1 (cytochrome P450, family 706, subfamily A, polypeptide 1 )

HSP 1 Score: 270.4 bits (690), Expect = 2.9e-72
Identity = 163/474 (34.39%), Postives = 261/474 (55.06%), Query Frame = 0

Query: 33  LPPGPLPVPIFGNWLQVGDDLNHRNLTDLAKKFGDIFLLRMGQRNLVVISSPELAKEVLH 92
           LPPGP P+PI GN   +  D+ H     L  K G +  + +G +  +V+SSP++A+EVL 
Sbjct: 84  LPPGPWPLPIVGNLPFLNSDVLHTQFQALTLKHGPLMKIHLGSKLAIVVSSPDMAREVLK 143

Query: 93  TQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQ-QYRQGWE 152
           T  + F +     V  I T  G+D++++ YG HWR++R++  +  FT   ++  Y    E
Sbjct: 144 THDITFANHDLPEVGKINTYGGEDILWSPYGTHWRRLRKLCVMKMFTTPTLEASYSTRRE 203

Query: 153 DEAQSVVDDVKKNPESATAGVVLRRRLQLMMYNNMYRIMFDRRFESED-DPLFHKLRALN 212
           +  Q++V   +   E +   V L  ++ L ++N + R+M+    E ++   L ++L+ L 
Sbjct: 204 ETRQTIVHMSEMAREGSP--VNLGEQIFLSIFNVVTRMMWGATVEGDERTSLGNELKTLI 263

Query: 213 GERSRLAQSFDYNYGDFIPILRPF-LRGYLKICK-EVKETRLKLFKDYFVEERKKLANTK 272
            + S +      NY DF P+   F  +G +K  K  VK  +L L  D  +E   K+   K
Sbjct: 264 SDISDIEGI--QNYSDFFPLFSRFDFQGLVKQMKGHVK--KLDLLFDRVMESHVKMVGKK 323

Query: 273 STTNEGLKCAIDHILDAQQKGEINEDNVLYIVENINVAAIETTLWSVEWGIAELVNHPEI 332
           S   E     +  + D  +K  ++  +V  ++ ++ +  ++T++ + E+ +AE+V+ PE+
Sbjct: 324 SEEEEDFLQYLLRVKDDDEKAPLSMTHVKSLLMDMVLGGVDTSVNASEFAMAEIVSRPEV 383

Query: 333 QRKLRNELDTVLGPGVPITEPDTHKLPYLQAVVKETLRLRMAIPLLVPHMNLHDAKLGGY 392
             K+R ELD V+G    + E    KLPYLQAV+KETLRL   +PLLVPH N   + + GY
Sbjct: 384 LNKIRLELDQVVGKDNIVEESHLPKLPYLQAVMKETLRLHPTLPLLVPHRNSETSVVAGY 443

Query: 393 DIPAESKILVNAWWLANNPANWKNPEEFRPERFLEEESKVEANGNDFRYLPFGVGRRSCP 452
            +P +SKI +N W +  +P NW  P EF+PERFL  E+ ++ NG DF+YLPFG GRR C 
Sbjct: 444 TVPKDSKIFINVWAIHRDPKNWDEPNEFKPERFL--ENSLDFNGGDFKYLPFGSGRRICA 503

Query: 453 GIILALPILGITIGRLVQNFELLPPPGQSKLDTSEKGGQFSLHILKHSTIVAKP 503
            I +A  ++   I  L+ +F+   P GQ K +  EK   F L +   S +VA P
Sbjct: 504 AINMAERLVLFNIASLLHSFDWKAPQGQ-KFEVEEK---FGLVLKLKSPLVAIP 545

BLAST of HG10001591 vs. TAIR 10
Match: AT4G22710.1 (cytochrome P450, family 706, subfamily A, polypeptide 2 )

HSP 1 Score: 270.0 bits (689), Expect = 3.8e-72
Identity = 170/498 (34.14%), Postives = 273/498 (54.82%), Query Frame = 0

Query: 11  IGLFLAVVLAIVI-SKLRGKRF-KLPPGPLPVPIFGNWLQVGDDLNHRNLTDLAKKFGDI 70
           +   LAVV  + I  K + K+   LPPGP P+PI GN   +  D+ H     L  K+G +
Sbjct: 29  VAALLAVVCYLWIQGKSKSKKEPPLPPGPWPLPIVGNLPFLNSDVLHTQFQALTLKYGPL 88

Query: 71  FLLRMGQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRK 130
             + +G +  +V+SSP++A+EVL T  + F +     V  I T  G+D++++ YG HWR+
Sbjct: 89  MKIHLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGGEDILWSPYGTHWRR 148

Query: 131 MRRIMTVPFFTNKVVQ-QYRQGWEDEAQSVVDDVKKNPESATAGVVLRRRLQLMMYNNMY 190
           +R++  +  FT   ++  Y    E+  Q++V   +   E +   V L  ++ L ++N + 
Sbjct: 149 LRKLCVMKMFTTPSLEASYSTRREETRQTIVHMSEMAREGSP--VNLGEQIFLSIFNVVT 208

Query: 191 RIMFDRRFESED-DPLFHKLRALNGERSRLAQSFDYNYGDFIPILRPF-LRGYLKICK-E 250
           R+M+    E ++   L ++L+ L  + S +      NY DF P+   F  +G +K  K  
Sbjct: 209 RMMWGATVEGDERTSLGNELKTLISDISDIEGI--QNYSDFFPLFSRFDFQGLVKQMKGH 268

Query: 251 VKETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGEINEDNVLYIVENIN 310
           VK  +L L  D  +E   K+   KS   E     +  + D  +K  ++  +V  ++ ++ 
Sbjct: 269 VK--KLDLLFDRVMESHVKMVGKKSEEEEDFLQYLLRVKDDDEKAPLSMTHVKSLLMDMV 328

Query: 311 VAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLGPGVPITEPDTHKLPYLQAVVKET 370
           +  ++T++ + E+ +AE+V+ PE+  K+R ELD V+G    + E    KLPYLQAV+KET
Sbjct: 329 LGGVDTSVNASEFAMAEIVSRPEVLNKIRLELDQVVGKDNIVEESHLPKLPYLQAVMKET 388

Query: 371 LRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPERFLEE 430
           LRL   +PLLVPH N   + + GY +P +SKI +N W +  +P NW  P EF+PERFL  
Sbjct: 389 LRLHPTLPLLVPHRNSETSVVAGYTVPKDSKIFINVWAIHRDPKNWDEPNEFKPERFL-- 448

Query: 431 ESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSKLDTSEK 490
           E+ ++ NG DF+YLPFG GRR C  I +A  ++   I  L+ +F+   P GQ K +  EK
Sbjct: 449 ENSLDFNGGDFKYLPFGSGRRICAAINMAERLVLFNIASLLHSFDWKAPQGQ-KFEVEEK 508

Query: 491 GGQFSLHILKHSTIVAKP 503
              F L +   S +VA P
Sbjct: 509 ---FGLVLKLKSPLVAIP 514

BLAST of HG10001591 vs. TAIR 10
Match: AT3G26230.1 (cytochrome P450, family 71, subfamily B, polypeptide 24 )

HSP 1 Score: 249.2 bits (635), Expect = 7.0e-66
Identity = 160/487 (32.85%), Postives = 259/487 (53.18%), Query Frame = 0

Query: 14  FLAVVLAIVISKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTDLAKKFGDIFLLRM 73
           F+A++  I+I K++     LPP PL +P+ GN  Q+   L H+ L DL+KK G + LLR+
Sbjct: 7   FIALLSLIIIKKIKDSNRNLPPSPLKLPVIGNLYQL-RGLFHKCLHDLSKKHGPVLLLRL 66

Query: 74  GQRNLVVISSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIM 133
           G  ++VVISS E A+E L    +E  +R    V       GQD+    YGE  R++R++ 
Sbjct: 67  GFLDMVVISSTEAAEEALKVHDLECCTRPITNVTSKLWRDGQDIGLAPYGESLRELRKLS 126

Query: 134 TVPFFTNKVVQQYRQGWEDEAQSVVDDVKKNPESA--TAGVVLRRRLQLMMYNNMYRIMF 193
            + FF+   V+ +R   E+E   +   VKK  E+A   + V L + L  ++ + ++R  F
Sbjct: 127 FLKFFSTTKVRSFRYIREEENDLM---VKKLKEAALKKSSVDLSQTLFGLVGSIIFRSAF 186

Query: 194 DRRFESEDDPLFHKLRALNGERSRL-----AQSFDYNYGDFIPILRPFLRGYLKICKEVK 253
            +RF+  +     K+  L  E  +L     +  F    G F+  +    +   K+  EV 
Sbjct: 187 GQRFDEGNHVNAEKIEDLMFEVQKLGALSNSDLFPGGLGWFVDFVSGHNKKLHKVFVEVD 246

Query: 254 ETRLKLFKDYFVEERKKLANTKSTTNEGLKCAIDHILDAQQKGE------INEDNVLYIV 313
                +  D+     +++ + +          ID +LD  +K E      +  DN+  I+
Sbjct: 247 TLLNHIIDDHLKNSIEEITHDRPD-------IIDSLLDMIRKQEQGDSFKLTIDNLKGII 306

Query: 314 ENINVAAIETTLWSVEWGIAELVNHPEIQRKLRNELDTVLG--PGVPITEPDTHKLPYLQ 373
           ++I +A ++T+  ++ W +AELV +P + +K+++E+ T +G      I E D  KL YL+
Sbjct: 307 QDIYLAGVDTSAITMIWAMAELVKNPRVMKKVQDEIRTCIGIKQNEKIEEDDVDKLQYLK 366

Query: 374 AVVKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRP 433
            VVKETLRL  A PLL+P   +   K+ GY+IP+++ +LVN W +  +P +WKNPEEF P
Sbjct: 367 LVVKETLRLHPAAPLLLPRETMSQIKIQGYNIPSKTILLVNVWSIGRDPKHWKNPEEFNP 426

Query: 434 ERFLEEESKVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNFELLPPPGQSK 486
           ERF+  +  ++  GN F  LPFG GRR CPGI  A+  + + +  L+ +F+   P     
Sbjct: 427 ERFI--DCPIDYKGNSFEMLPFGSGRRICPGIAFAIATVELGLLNLLYHFDWRLPEEDKD 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008456253.17.3e-29198.02PREDICTED: trans-cinnamate 4-monooxygenase [Cucumis melo] >KAA0033521.1 trans-ci... [more]
XP_038901578.13.6e-29098.22trans-cinnamate 4-monooxygenase [Benincasa hispida][more]
KGN46771.21.7e-28796.83hypothetical protein Csa_020744 [Cucumis sativus][more]
NP_001292707.13.7e-28796.83trans-cinnamate 4-monooxygenase [Cucumis sativus] >CAK95273.1 cinnamate-4-hydrox... [more]
XP_023007159.13.0e-28495.84trans-cinnamate 4-monooxygenase [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q430543.6e-27791.09Trans-cinnamate 4-monooxygenase OS=Populus kitakamiensis OX=34292 GN=CYP73A16 PE... [more]
O243125.2e-27690.69Trans-cinnamate 4-monooxygenase OS=Populus tremuloides OX=3693 GN=CYP73A13 PE=2 ... [more]
Q964231.3e-27490.30Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza echinata OX=46348 GN=CYP73A14 PE=... [more]
P371154.6e-27289.11Trans-cinnamate 4-monooxygenase OS=Vigna radiata var. radiata OX=3916 GN=CYP73A2... [more]
F1B2831.0e-27188.91Trans-cinnamate 4-monooxygenase C4H2 OS=Petunia hybrida OX=4102 GN=C4H2 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A5D3B9663.5e-29198.02Trans-cinnamate 4-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3C2S53.5e-29198.02trans-cinnamate 4-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103496249 PE=3 SV=... [more]
Q17UC01.8e-28796.83Cinnamate-4-hydroxylase OS=Cucumis sativus OX=3659 GN=c4H PE=2 SV=1[more]
A0A6J1L4701.4e-28495.84trans-cinnamate 4-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499735 PE=3... [more]
A0A6J1G7485.4e-28495.64trans-cinnamate 4-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111451474 PE... [more]
Match NameE-valueIdentityDescription
AT2G30490.11.6e-26487.08cinnamate-4-hydroxylase [more]
AT2G40890.16.7e-7736.17cytochrome P450, family 98, subfamily A, polypeptide 3 [more]
AT4G22690.12.9e-7234.39cytochrome P450, family 706, subfamily A, polypeptide 1 [more]
AT4G22710.13.8e-7234.14cytochrome P450, family 706, subfamily A, polypeptide 2 [more]
AT3G26230.17.0e-6632.85cytochrome P450, family 71, subfamily B, polypeptide 24 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 62..81
score: 37.46
coord: 295..312
score: 29.84
coord: 181..199
score: 29.27
coord: 315..341
score: 38.75
coord: 86..107
score: 33.06
coord: 399..423
score: 40.41
coord: 358..376
score: 40.87
coord: 437..447
score: 56.69
coord: 447..470
score: 35.64
IPR001128Cytochrome P450PRINTSPR00385P450coord: 359..370
score: 43.85
coord: 438..447
score: 60.86
coord: 306..323
score: 29.77
IPR001128Cytochrome P450PFAMPF00067p450coord: 34..495
e-value: 4.8E-117
score: 391.5
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 27..504
e-value: 4.5E-115
score: 386.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 34..503
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 8..458
e-value: 2.4E-19
score: 67.4
NoneNo IPR availablePIRSRPIRSR000209-1PIRSR000209-1coord: 35..472
e-value: 2.2E-38
score: 129.4
NoneNo IPR availablePANTHERPTHR47948TRANS-CINNAMATE 4-MONOOXYGENASEcoord: 1..505
NoneNo IPR availablePANTHERPTHR47948:SF2TRANS-CINNAMATE 4-MONOOXYGENASEcoord: 1..505
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 440..449

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10001591.1HG10001591.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009808 lignin metabolic process
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0140359 ABC-type transporter activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016710 trans-cinnamate 4-monooxygenase activity
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen