HG10001415 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10001415
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionProtein kinase domain-containing protein
LocationChr09: 16930831 .. 16935701 (+)
RNA-Seq ExpressionHG10001415
SyntenyHG10001415
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTGCTTTAGCCATATCAAAAAACAGAGGAAAAGATTATTAAGGAGCAATGTACAAAGCATACAACCAGCTTCTCCAGGTAATTTTTATCTAATTTTCAAATTATTTAAGGTATTTTCTATTCAAGGGGTCCATTTGATTTGAAGATTGATGCGTTGTCGTTTTGATATTGTTCCCCACATGGGTCCAGAAAATCGTTCAATAGGACAAATTTATGATTGATTTTGGTTATTTTAATTTTATTTCTTGATTACTTTTTGTGTGATTTTAGTTTTGGATGAACAATTTCTATTAAAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAATCTTACATAGATTTTTACTGATTTTCTGTTTGGTTTATGTTTGCTTGGAAAAGAAAATATTCCCTCTGTCTCGTCTTTCAAATTCTGGAAATGTTGGGATTTTTGCTTTGCTTGTGAATCAATCAAGGAAGGTTTTGGTTCATAATTCCTCTTTTAGAGATTTTTCTTTGGCCAAAATGCAACTAATCTTTGATGTAAAGATTGTAATTTATAAAAACCAAGATGATAACAGAAGAATTGGAGTTCTAAACTTCCATGCATATGGTTTGAATTTGTGCATAGTCTATAACATTAAAAAAAATTCATAATTTGAGAAATTTAGTAAACATTTAAGACATGTTTCACTCTTAAAAGTTTGTGATTGTGATACCACAACTAAAATTTTTTAATTTCACCATTTATTAATTTCATAGGAGTCTCATATCTAAATAAATAACTCTTTAGACTTAGCAACTCTTCAAACTAAACTTCACTCATTACCTCAAATGTCTACTTAAGCAATTTCAATTTAGGGAAATTTTTAAATATTTACACAAAATAGTAAAGATTTTAGATAGTTGTGATAGACGCTGATAGAAGTCTATCCGTGATAAAAATGATAGAAGTTTATCACTGATAGATGTGGATAGAAATCTATCAATGTCTATCAGTGTTTTCTTTTGCTATTTTTTGTAAATAGTTTGACATTTTTTTTATTTGTGAAATTTTTCTTTATTAATTTATGCTTTAATGAAATTCCTTTTAAAGGAAAAATGAAAAAGTAAAATTTAACTACACAACTTCAAATTTATTTTAAATAATTCCTCTTAAAGTTAAATAACACACCATTCATTTGCGTGTTATTTTTATTAATCGTGTTTTTTTAGATAATAAATATTTTTTAACGGCTGCAATATACTATTCCTCAACTTTCATAAACATTTTAAAAGAATTCTTCGAATATAAATCTTTTAAAATTTCAAACCGTAATTAAGACTTAAAACTGTGTGACACTAACTTAAAATGATGTGGTGGTCTCAAAGTCTAAAGAATTTTTAGTCTAAATGTATTTTTGAAACGTGTTTTCATATAGTTTAACCTTTTTTTTTTTACCCCGGGTTTGACCAATACAGTAAAAGCGGATCTGAAAGTAAAAAGAGGGGAAGAAATAAAAGAGGTAGTTTATGAAAATGAGCAGCTGGCGTCAAATATAAACGAAGAGGTTTCCAGTGAAGTAAAAACAAAGGAAGAGATAGAAGCATTTACATTCCAGGAACTAGCAGCAGCAACAAAAAATTTCAAGTCGGATTGCTTTCTGGGTGAAGGAGGATTTGGTAAAGTTTACAAGGGCCATTTGAGAAGAATCAATAAGGTAAATTTTTTATCAGTTTTCAAATTAATCCACTCATCATATTATTATAGTATTATTTAGTATGTACTGACATGTCACTTATAATATTGAAATAATTTTCATCATCAACTTTTAATATTAAATTTATTTAAGGGATAATTGTAAATATAACAATCAGATTCAAAGTATTAGCATATATAGCAACATTTTAAAAAGAGTTACAAATATAGCAAAGTTTATCTATGATAGACTTCTATCGCTGATAGACTTTTATGGCCTAAGTGGTTTATCACTGATAGACCATAAGAGTTTATCAGTGATAGAAGACTTCGCTGTATTTGCAATTTTTTTTAAAATTTTGTTTTACACTTTGTTATTATTCCTAAAATTGCTACTCTTTATTTAATCTATGTTGATTTCTATTTAAATCTTTAAACTTTTAAAATTTAACAATGCTCATTAAAATATCTTTTTTGTCCATGTACTTTGAGGCTTACTCAATTTTAGTTTTTCTACTTTCAAATCTTCAATTTTAGTCCTTATACATTCAAGAAGTCTTAATTCAATCCCCCCAAGTTGTTTTTATCTAAATTAGTTATGCTAGAAAATATAATATATATGAATATATATTTTCAAAATTTACCGTAAAAATGGTAATTATAGATTTTTTTAAATTTTTAAAACCAACAATAGTCTAGCAATATATACTAAATTTTAGATTTATTGAAAGTAAGGATTAAAATTGGATATTTAAAAGTACATAAACTAAAAATTGAATAGGTCTCAAAGTACAGTGACCAAAACGATATTTTAACCTTAAATTTTTAATTACGTGACTACTAAATTTTTAGCATTGGCAATATGGACTTATTAGATACAAAATTTAAAACTTTAATATTTGTTAGAATCTTTTGAATTAATCATTTGGTCTTTACTTTTTTTTAAATTACTTGTTTAATTCTATCACTATTTCTCTACTTAGCTTCAACGTTATTGTCAAAGCAAGTATATCTCAACTAATATACGAATGTGTTAGCTACCAATAGGTCTATGATTCGAATTTCTTTATCTTTTATCTTTTATGATTTGAATTTTTTAATTTTTTTGATATGACATTTTAAAAACTAAGAGAAATTGAAATTGATATTGTTTGTTGGACTCTAATTTTGATATAGCTTGTAGCTATAAAGCAACTAGATCGGAATGGAGTTCAAGGGGTAAGAGAATTCGTGGTGGAAGTGGTGACATTAAGTTTGGCGAATCACCCAAATCTTGTAAAATTGATTGGGTATTGTGCTGATGGTGATCAAAGACTCTTGGTTTATGAGTTCATGCCATTAGGCTCATTGGACAAACATTTGCACGGTACGTTGTTTATTCCTTTCCCTTCCAATTTTTCCTCTCAAAAAAATTAAATTACTAATTTAGTTTTTTAACCTTTAAATTTATATTTATTTGATCAATAAAGTTTTAAACGTATTAATAGATTCATAGATTTTAAATTTAGTGTATACTAAGTCTTTAATATGTTTAATTTTTTTTCAGTAGATATATAAGATTGAATTAATTTATGTTTGATGGAACTTTAAATTTTCAATTTTGATTTCATGTATGTATGTGGATTTTTAAAATGTTGAATAGGACAAAGACTTATTAGACACATTAAACACAAGATTGAGAGCTTGAGGGCCTATTTTTGACATTTTTTTATTGGACATAAAATTGAAACCTCAAAGAGACTTATTAAATTAAATACGTTTAGAAGTTCAAATGCCATAAATCTTTTCCTAAAAAAAAAATAAAAAAAATCTAGAAGTTCAATGACAAACACCTTATAATTTTGAATATCATCTTAGTTCTTATACTTTGAGACATCTTCAATTTTGTTACATATACTTTTAAATGTCTAATTTTAGTCTTAAACTTTTAATGAATCTTAATTTTTAATCACTCAAAGTTATTTTTTTTCTTCGAAATTAGTTAAATAATAATAATAATTTTTTGGATTGTTTTCAAATATAGAAAAATGAGTCAAACTATTTACAAATATAAAAAAAATTTATAGTCTATAAGTGATAGACTGCGATAGAATTCTATCGCTTTCTATTGCTCAAGCAAATACACTTCAAAATGACTTATTTTTAAAATAGACTGCGATAGAATTCTTTAATATGTTTAATTTTTTCGGTAGATATATAAAATACACTTCAAAAGCTATTTTGAATAGTTGCCAAACACTCTATTTTTTTTCTTTCAAAATGACTTATTTTTTTTTAAATTAAACATTTAAAAATGTATTCCAAACACACACTTAAAGCGTGTTTGGATTGGCTTAAGAAAAAAGTGTTTTTCAGAAAGCTCATTTTATTTAAACTCTTTTAATAAAAAACTATTTAAAATACACTTCAAAATCTATTTTGAGTGGTTACCAAACACTCCAATTTTTTTTTTTCCAAAATGACTTATTTTTTAAATTAAACACTTGAAAATGTATTCCAAACACAAACTTAGACCCGTTTGGATTCACTTGAGAAAAAAATGTTTTTTAAAAAATTCATTTTTATTTAAACTCTTTTGATAAAAACTGTTTAAAATACACTACAAAAGCTATTTTGAGTAGTTGCCAAACACTTAAATTCTTTTCAAAATGGCTTATTTTTTAAAATTAAACACTTAAAATGTATTCCAAATACACACTTAGTTGTGCTTAAATTAACAATTTAACTTTTTTTAAGAGATTATTTCCATCTAATGTTAAGATTGATGTTTTGTTGGGGCTTTGTATAGATGTTCCACCAGGCAAAAAAGTACTTGATTGGAATACAAGAATGAAGATAGCAGTGGGAGCAGCAAAAGGATTAGAATATTTACACAACAAAATGAGGCCAGCAGTTATATATAGAGATTTAAAATGCTCTAATATTTTGCTGGGTGAAGGTTATTGGCCAAAGCTTTCAGATTTTGGTCTAGCCAAAGTGGGTCCTAGTGGAGATAATACTCATGTTTCTACTAGAGTTATGGGAACTTATGGATATTGTGCACCAGATTATGCAATGACTGGCCAATTGACATTCAAATGTGATATCTATAGCTTTGGAGTTGTTCTATTGGAACTCATCACTGGAAGAAAAGCCATTGACCATACAAGGCCACCAAAGGAGCTAAGCTTGGTAGCATGGGTGAGTTATTTAACCAAACTTAATATTCCATGA

mRNA sequence

ATGGGTTGCTTTAGCCATATCAAAAAACAGAGGAAAAGATTATTAAGGAGCAATGTACAAAGCATACAACCAGCTTCTCCAGTAAAAGCGGATCTGAAAGTAAAAAGAGGGGAAGAAATAAAAGAGGTAGTTTATGAAAATGAGCAGCTGGCGTCAAATATAAACGAAGAGGTTTCCAGTGAAGTAAAAACAAAGGAAGAGATAGAAGCATTTACATTCCAGGAACTAGCAGCAGCAACAAAAAATTTCAAGTCGGATTGCTTTCTGGGTGAAGGAGGATTTGGTAAAGTTTACAAGGGCCATTTGAGAAGAATCAATAAGCTTGTAGCTATAAAGCAACTAGATCGGAATGGAGTTCAAGGGGTAAGAGAATTCGTGGTGGAAGTGGTGACATTAAGTTTGGCGAATCACCCAAATCTTGTAAAATTGATTGGGTATTGTGCTGATGGTGATCAAAGACTCTTGGTTTATGAGTTCATGCCATTAGGCTCATTGGACAAACATTTGCACGATGTTCCACCAGGCAAAAAAGTACTTGATTGGAATACAAGAATGAAGATAGCAGTGGGAGCAGCAAAAGGATTAGAATATTTACACAACAAAATGAGGCCAGCAGTTATATATAGAGATTTAAAATGCTCTAATATTTTGCTGGGTGAAGGTTATTGGCCAAAGCTTTCAGATTTTGGTCTAGCCAAAGTGGGTCCTAGTGGAGATAATACTCATGTTTCTACTAGAGTTATGGGAACTTATGGATATTGTGCACCAGATTATGCAATGACTGGCCAATTGACATTCAAATGTGATATCTATAGCTTTGGAGTTGTTCTATTGGAACTCATCACTGGAAGAAAAGCCATTGACCATACAAGGCCACCAAAGGAGCTAAGCTTGGTAGCATGGGTGAGTTATTTAACCAAACTTAATATTCCATGA

Coding sequence (CDS)

ATGGGTTGCTTTAGCCATATCAAAAAACAGAGGAAAAGATTATTAAGGAGCAATGTACAAAGCATACAACCAGCTTCTCCAGTAAAAGCGGATCTGAAAGTAAAAAGAGGGGAAGAAATAAAAGAGGTAGTTTATGAAAATGAGCAGCTGGCGTCAAATATAAACGAAGAGGTTTCCAGTGAAGTAAAAACAAAGGAAGAGATAGAAGCATTTACATTCCAGGAACTAGCAGCAGCAACAAAAAATTTCAAGTCGGATTGCTTTCTGGGTGAAGGAGGATTTGGTAAAGTTTACAAGGGCCATTTGAGAAGAATCAATAAGCTTGTAGCTATAAAGCAACTAGATCGGAATGGAGTTCAAGGGGTAAGAGAATTCGTGGTGGAAGTGGTGACATTAAGTTTGGCGAATCACCCAAATCTTGTAAAATTGATTGGGTATTGTGCTGATGGTGATCAAAGACTCTTGGTTTATGAGTTCATGCCATTAGGCTCATTGGACAAACATTTGCACGATGTTCCACCAGGCAAAAAAGTACTTGATTGGAATACAAGAATGAAGATAGCAGTGGGAGCAGCAAAAGGATTAGAATATTTACACAACAAAATGAGGCCAGCAGTTATATATAGAGATTTAAAATGCTCTAATATTTTGCTGGGTGAAGGTTATTGGCCAAAGCTTTCAGATTTTGGTCTAGCCAAAGTGGGTCCTAGTGGAGATAATACTCATGTTTCTACTAGAGTTATGGGAACTTATGGATATTGTGCACCAGATTATGCAATGACTGGCCAATTGACATTCAAATGTGATATCTATAGCTTTGGAGTTGTTCTATTGGAACTCATCACTGGAAGAAAAGCCATTGACCATACAAGGCCACCAAAGGAGCTAAGCTTGGTAGCATGGGTGAGTTATTTAACCAAACTTAATATTCCATGA

Protein sequence

MGCFSHIKKQRKRLLRSNVQSIQPASPVKADLKVKRGEEIKEVVYENEQLASNINEEVSSEVKTKEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQLDRNGVQGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDVPPGKKVLDWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPPKELSLVAWVSYLTKLNIP
Homology
BLAST of HG10001415 vs. NCBI nr
Match: XP_038900307.1 (probable serine/threonine-protein kinase PBL5 [Benincasa hispida] >XP_038900308.1 probable serine/threonine-protein kinase PBL5 [Benincasa hispida])

HSP 1 Score: 545.4 bits (1404), Expect = 3.1e-151
Identity = 270/303 (89.11%), Postives = 283/303 (93.40%), Query Frame = 0

Query: 1   MGCFSHIKKQRKRLLRSNVQSIQPAS--PVKADLKVKRGEEIKEVVYENEQLASNINEEV 60
           MGCFSHIKKQRKRLL SN+Q+IQPAS   VKADLKVK  E++KE V+ENEQLASNIN+EV
Sbjct: 23  MGCFSHIKKQRKRLLGSNLQNIQPASSVKVKADLKVKTREQVKEEVHENEQLASNINKEV 82

Query: 61  SSEVKTKEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQLDRNG 120
           SSE K   EIE FTFQEL AAT+NFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQLDR+G
Sbjct: 83  SSEAKIDREIETFTFQELVAATRNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQLDRSG 142

Query: 121 VQGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDVPPGKKV 180
            QGVREF+VEVVTLSLANHPNLVKLIGYCA+G QRLLVYEFMPLGSLDKHLHDVP GK+V
Sbjct: 143 DQGVREFLVEVVTLSLANHPNLVKLIGYCAEGHQRLLVYEFMPLGSLDKHLHDVPAGKRV 202

Query: 181 LDWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSG 240
           LDWNTRMKIAVGAA+GLEYLHNKMRPAVIYRDLK SNILLGEGYWPKLSDFGLAKVGPSG
Sbjct: 203 LDWNTRMKIAVGAARGLEYLHNKMRPAVIYRDLKSSNILLGEGYWPKLSDFGLAKVGPSG 262

Query: 241 DNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPPKELSL 300
           DNTHVSTRVMGTYGYCAPDYAMTGQLTFK DIYSFGVVLLELI+GRK IDHTRP KELSL
Sbjct: 263 DNTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELISGRKVIDHTRPSKELSL 322

Query: 301 VAW 302
           VAW
Sbjct: 323 VAW 325

BLAST of HG10001415 vs. NCBI nr
Match: XP_022986891.1 (probable serine/threonine-protein kinase PBL5 [Cucurbita maxima])

HSP 1 Score: 516.5 bits (1329), Expect = 1.6e-142
Identity = 252/306 (82.35%), Postives = 275/306 (89.87%), Query Frame = 0

Query: 1   MGCFSHIKKQRKRL-LRSNVQSIQPASPVKADLKVKRGEEIKEVVYENEQLASNINEEVS 60
           MGCF H KKQRK+  + S + S++    V+ADLKV+  EE+KE   EN+QLASNIN EVS
Sbjct: 1   MGCFRHSKKQRKKFEINSPISSVK----VEADLKVETREEVKEQTRENDQLASNINREVS 60

Query: 61  SEVKTKEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQLDRNGV 120
            +VKT EEIE FTF EL  AT+NFKSDCFLGEGGFGKVYKGHL RI+++VAIKQLDRNG+
Sbjct: 61  GKVKTNEEIEKFTFHELVEATRNFKSDCFLGEGGFGKVYKGHLIRIDQVVAIKQLDRNGL 120

Query: 121 QGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDVPPGKKVL 180
           QGVREFVVEV+TLSLA+HPNLVKL+GYCA+ D+RLLVYEFMPLGSLDKHLHD+PPGK VL
Sbjct: 121 QGVREFVVEVLTLSLADHPNLVKLLGYCAERDERLLVYEFMPLGSLDKHLHDLPPGKNVL 180

Query: 181 DWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSGD 240
           DWNTRMKIAVGAA+GLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSGD
Sbjct: 181 DWNTRMKIAVGAARGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSGD 240

Query: 241 NTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPPKELSLV 300
           NTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRK ID+TRPPKELSLV
Sbjct: 241 NTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKVIDYTRPPKELSLV 300

Query: 301 AWVSYL 306
            W   L
Sbjct: 301 TWAKPL 302

BLAST of HG10001415 vs. NCBI nr
Match: XP_004148248.1 (probable serine/threonine-protein kinase PBL5 [Cucumis sativus] >KGN47330.1 hypothetical protein Csa_022976 [Cucumis sativus])

HSP 1 Score: 515.4 bits (1326), Expect = 3.5e-142
Identity = 259/308 (84.09%), Postives = 274/308 (88.96%), Query Frame = 0

Query: 1   MGCFSHIKKQRKRLLRSNVQSIQPAS-----PVKADLKVKRGEEIKEVVYENE-QLASNI 60
           MGCFSHI+KQ+KRL  S ++SIQPAS      VK DLKVK  EE KEVVYENE +L SNI
Sbjct: 1   MGCFSHIRKQKKRLSGSKLESIQPASSGNAVKVKRDLKVKTREEAKEVVYENEHRLGSNI 60

Query: 61  NEEVSSEVKT-KEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQ 120
            +EV  E KT  EE++ FTF ELA AT+NFKSDCFLGEGGFGKVYKG+L RINKLVAIKQ
Sbjct: 61  KKEVCGEAKTSNEEVKTFTFHELAEATRNFKSDCFLGEGGFGKVYKGYLARINKLVAIKQ 120

Query: 121 LDRNGVQGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDVP 180
           LD +  QG+REF+VEVVTLSLAN PNLVKLIGYC +GDQRLLVYEFMPLGSLDKHLHDVP
Sbjct: 121 LDWSRGQGIREFMVEVVTLSLANDPNLVKLIGYCVEGDQRLLVYEFMPLGSLDKHLHDVP 180

Query: 181 PGKKVLDWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAK 240
            G KVLDWNTRMKIAVGAAKGLEYLHNKM+PAVIYRDLKCSNILLGE YWPKLSDFGLAK
Sbjct: 181 AGNKVLDWNTRMKIAVGAAKGLEYLHNKMKPAVIYRDLKCSNILLGEDYWPKLSDFGLAK 240

Query: 241 VGPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPP 300
           VGPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRP 
Sbjct: 241 VGPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPS 300

Query: 301 KELSLVAW 302
           KELSLVAW
Sbjct: 301 KELSLVAW 308

BLAST of HG10001415 vs. NCBI nr
Match: XP_023511984.1 (probable serine/threonine-protein kinase PBL5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 513.8 bits (1322), Expect = 1.0e-141
Identity = 249/301 (82.72%), Postives = 271/301 (90.03%), Query Frame = 0

Query: 1   MGCFSHIKKQRKRLLRSNVQSIQPASPVKADLKVKRGEEIKEVVYENEQLASNINEEVSS 60
           MGCF H KKQRK+    +  S   +  ++ADL+VK GEE+KE   EN+QLASNI  EVSS
Sbjct: 1   MGCFRHSKKQRKKF---DSNSPISSGNLEADLRVKTGEEVKEQTRENDQLASNITREVSS 60

Query: 61  EVKTKEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQLDRNGVQ 120
           + KT EEIE FTF EL  AT+NFKSDCFLGEGGFGKVYKGHL RI+++VAIKQLDRNG+Q
Sbjct: 61  KAKTNEEIEKFTFHELVEATRNFKSDCFLGEGGFGKVYKGHLIRIDQVVAIKQLDRNGLQ 120

Query: 121 GVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDVPPGKKVLD 180
           GVREFVVEV+TLSLA+HPNLVKL+GYCA+ D+RLLVYEFMPLGSLDKHLHD+PPGK VLD
Sbjct: 121 GVREFVVEVLTLSLADHPNLVKLLGYCAERDERLLVYEFMPLGSLDKHLHDLPPGKNVLD 180

Query: 181 WNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSGDN 240
           WNTRMKIAVGAA+GLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSGDN
Sbjct: 181 WNTRMKIAVGAARGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSGDN 240

Query: 241 THVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPPKELSLVA 300
           THVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRK ID+TRPPKELSLV 
Sbjct: 241 THVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKVIDYTRPPKELSLVT 298

Query: 301 W 302
           W
Sbjct: 301 W 298

BLAST of HG10001415 vs. NCBI nr
Match: XP_016903103.1 (PREDICTED: probable serine/threonine-protein kinase RLCKVII [Cucumis melo])

HSP 1 Score: 510.4 bits (1313), Expect = 1.1e-140
Identity = 257/307 (83.71%), Postives = 271/307 (88.27%), Query Frame = 0

Query: 1   MGCFSHIKKQRKRLLRSNVQSIQPAS-----PVKADLKVKRGEEIKEVVYENE-QLASNI 60
           MGCFSHIKKQRKRLL S +QSIQPAS      V+ +LKVK  EE KEVV EN+ +L SNI
Sbjct: 1   MGCFSHIKKQRKRLLGSKLQSIQPASSANEVKVRRELKVKTREEAKEVVCENDHRLGSNI 60

Query: 61  NEEVSSEVKTKEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQL 120
           N+ V SE K KEE++ FTF ELA AT NFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQL
Sbjct: 61  NKGVCSEAKAKEEVKTFTFHELAEATGNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQL 120

Query: 121 DRNGVQGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDVPP 180
           D +  QG+REF+VEVVTLSLAN PNLVKLIGYC +GDQRLLVYEFMPLGSLD+HLHDVP 
Sbjct: 121 DWSRGQGIREFMVEVVTLSLANDPNLVKLIGYCVEGDQRLLVYEFMPLGSLDRHLHDVPA 180

Query: 181 GKKVLDWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKV 240
           G+KVLDWNTRM IAVGAAKGLEYLH KM PAVIYRDLKCSNILLGEGYW KLSDFGLAKV
Sbjct: 181 GEKVLDWNTRMNIAVGAAKGLEYLHTKMSPAVIYRDLKCSNILLGEGYWAKLSDFGLAKV 240

Query: 241 GPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPPK 300
           GPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFK DIYSFGVVLLELITGRKAIDHTRP  
Sbjct: 241 GPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELITGRKAIDHTRPSN 300

Query: 301 ELSLVAW 302
           ELSLVAW
Sbjct: 301 ELSLVAW 307

BLAST of HG10001415 vs. ExPASy Swiss-Prot
Match: Q9LQQ8 (Probable serine/threonine-protein kinase PBL5 OS=Arabidopsis thaliana OX=3702 GN=PBL5 PE=2 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 6.6e-112
Identity = 194/255 (76.08%), Postives = 223/255 (87.45%), Query Frame = 0

Query: 53  NINEEVSSEVKTKEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIK 112
           N+N++V     T ++ + FTFQELA AT NF+SDCFLGEGGFGKV+KG + +++++VAIK
Sbjct: 78  NLNDQV-----TGKKAQTFTFQELAEATGNFRSDCFLGEGGFGKVFKGTIEKLDQVVAIK 137

Query: 113 QLDRNGVQGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDV 172
           QLDRNGVQG+REFVVEV+TLSLA+HPNLVKLIG+CA+GDQRLLVYE+MP GSL+ HLH +
Sbjct: 138 QLDRNGVQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMPQGSLEDHLHVL 197

Query: 173 PPGKKVLDWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLA 232
           P GKK LDWNTRMKIA GAA+GLEYLH++M P VIYRDLKCSNILLGE Y PKLSDFGLA
Sbjct: 198 PSGKKPLDWNTRMKIAAGAARGLEYLHDRMTPPVIYRDLKCSNILLGEDYQPKLSDFGLA 257

Query: 233 KVGPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRP 292
           KVGPSGD THVSTRVMGTYGYCAPDYAMTGQLTFK DIYSFGVVLLELITGRKAID+T+ 
Sbjct: 258 KVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELITGRKAIDNTKT 317

Query: 293 PKELSLVAWVSYLTK 308
            K+ +LV W   L K
Sbjct: 318 RKDQNLVGWARPLFK 327

BLAST of HG10001415 vs. ExPASy Swiss-Prot
Match: Q9SIB6 (Probable serine/threonine-protein kinase PBL6 OS=Arabidopsis thaliana OX=3702 GN=PBL6 PE=2 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 9.5e-111
Identity = 208/323 (64.40%), Postives = 239/323 (73.99%), Query Frame = 0

Query: 1   MGCFSHIKKQRKR--------------LLRSNVQSIQPASPVKADLKVK-RGEEIKEVVY 60
           MGCF    K  KR               L  N    +   P    LKV   G+  KE+V 
Sbjct: 1   MGCFGRTPKSNKRSDTKTTKNNDFTPKKLTVNANRDKLTQPSSDCLKVSICGDVSKEIVT 60

Query: 61  ENEQLASNINE-EVSSEVKTKEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRR 120
           + +QLA +  +  V  EV  K + + FTF+EL+ +T NFKSDCFLGEGGFGKVYKG + +
Sbjct: 61  KKDQLALDAKDTNVEDEVIVK-KAQTFTFEELSVSTGNFKSDCFLGEGGFGKVYKGFIEK 120

Query: 121 INKLVAIKQLDRNGVQGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGS 180
           IN++VAIKQLDRNG QG+REFVVEV+TLSLA+HPNLVKLIG+CA+G QRLLVYE+MPLGS
Sbjct: 121 INQVVAIKQLDRNGAQGIREFVVEVLTLSLADHPNLVKLIGFCAEGVQRLLVYEYMPLGS 180

Query: 181 LDKHLHDVPPGKKVLDWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWP 240
           LD HLHD+P GK  L WNTRMKIA GAA+GLEYLH+ M+P VIYRDLKCSNIL+ EGY  
Sbjct: 181 LDNHLHDLPSGKNPLAWNTRMKIAAGAARGLEYLHDTMKPPVIYRDLKCSNILIDEGYHA 240

Query: 241 KLSDFGLAKVGPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGR 300
           KLSDFGLAKVGP G  THVSTRVMGTYGYCAPDYA+TGQLTFK D+YSFGVVLLELITGR
Sbjct: 241 KLSDFGLAKVGPRGSETHVSTRVMGTYGYCAPDYALTGQLTFKSDVYSFGVVLLELITGR 300

Query: 301 KAIDHTRPPKELSLVAWVSYLTK 308
           KA D+TR     SLV W + L K
Sbjct: 301 KAYDNTRTRNHQSLVEWANPLFK 322

BLAST of HG10001415 vs. ExPASy Swiss-Prot
Match: Q6I5Q6 (Receptor-like cytoplasmic kinase 185 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK185 PE=1 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 1.6e-105
Identity = 183/237 (77.22%), Postives = 203/237 (85.65%), Query Frame = 0

Query: 71  FTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQLDRNGVQGVREFVVEVV 130
           FTF+ELAAATKNF+ DC LGEGGFG+VYKGHL    + VA+KQLDRNG+QG REF+VEV+
Sbjct: 68  FTFRELAAATKNFRQDCLLGEGGFGRVYKGHLEN-GQAVAVKQLDRNGLQGNREFLVEVL 127

Query: 131 TLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDVPPGKKVLDWNTRMKIAVG 190
            LSL +H NLV LIGYCADGDQRLLVYEFMPLGSL+ HLHD+PP K+ LDWNTRMKIA G
Sbjct: 128 MLSLLHHDNLVNLIGYCADGDQRLLVYEFMPLGSLEDHLHDIPPDKEPLDWNTRMKIAAG 187

Query: 191 AAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSGDNTHVSTRVMGT 250
           AAKGLE+LH+K  P VIYRD K SNILLGEGY PKLSDFGLAK+GP GD THVSTRVMGT
Sbjct: 188 AAKGLEFLHDKANPPVIYRDFKSSNILLGEGYHPKLSDFGLAKLGPVGDKTHVSTRVMGT 247

Query: 251 YGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPPKELSLVAWVSYLTK 308
           YGYCAP+YAMTGQLT K D+YSFGVV LELITGRKAID+T+P  E +LVAW   L K
Sbjct: 248 YGYCAPEYAMTGQLTVKSDVYSFGVVFLELITGRKAIDNTKPLGEQNLVAWARPLFK 303

BLAST of HG10001415 vs. ExPASy Swiss-Prot
Match: Q1PDV6 (Serine/threonine-protein kinase PBL27 OS=Arabidopsis thaliana OX=3702 GN=PBL27 PE=1 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.3e-104
Identity = 190/298 (63.76%), Postives = 222/298 (74.50%), Query Frame = 0

Query: 17  SNVQSIQPASPVKADLKVKRGEEIKEVVYENEQLASNINEEVSSEVKTKEE-------IE 76
           S+ +       VK +L  K G   +      ++  S    E   E+   +E        +
Sbjct: 10  SSAKDAASKDSVKKELSAKDGSVTQSHHISLDKSKSRRGPEQKKELTAPKEGPTAHIAAQ 69

Query: 77  AFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQLDRNGVQGVREFVVEV 136
            FTF+ELAAATKNF+ +C LGEGGFG+VYKG L    ++VA+KQLDRNG+QG REF+VEV
Sbjct: 70  TFTFRELAAATKNFRPECLLGEGGFGRVYKGRLETTGQIVAVKQLDRNGLQGNREFLVEV 129

Query: 137 VTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDVPPGKKVLDWNTRMKIAV 196
           + LSL +HPNLV LIGYCADGDQRLLVYE+MPLGSL+ HLHD+PP K+ LDW+TRM IA 
Sbjct: 130 LMLSLLHHPNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLHDLPPDKEPLDWSTRMTIAA 189

Query: 197 GAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSGDNTHVSTRVMG 256
           GAAKGLEYLH+K  P VIYRDLK SNILLG+GY PKLSDFGLAK+GP GD THVSTRVMG
Sbjct: 190 GAAKGLEYLHDKANPPVIYRDLKSSNILLGDGYHPKLSDFGLAKLGPVGDKTHVSTRVMG 249

Query: 257 TYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPPKELSLVAWVSYLTK 308
           TYGYCAP+YAMTGQLT K D+YSFGVV LELITGRKAID+ R P E +LVAW   L K
Sbjct: 250 TYGYCAPEYAMTGQLTLKSDVYSFGVVFLELITGRKAIDNARAPGEHNLVAWARPLFK 307

BLAST of HG10001415 vs. ExPASy Swiss-Prot
Match: Q0WRY5 (Probable serine/threonine-protein kinase PBL7 OS=Arabidopsis thaliana OX=3702 GN=PBL7 PE=1 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 1.2e-102
Identity = 181/264 (68.56%), Postives = 214/264 (81.06%), Query Frame = 0

Query: 46  ENEQLASNINEEVSSEVKTKEEI--EAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLR 105
           E  +  S+++E   S+ K  + I  + FTF ELA AT+NF+ +C +GEGGFG+VYKG+L 
Sbjct: 37  EKSRAKSSLSE---SKSKGSDHIVAQTFTFSELATATRNFRKECLIGEGGFGRVYKGYLA 96

Query: 106 RINKLVAIKQLDRNGVQGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLG 165
             ++  AIKQLD NG+QG REF+VEV+ LSL +HPNLV LIGYCADGDQRLLVYE+MPLG
Sbjct: 97  STSQTAAIKQLDHNGLQGNREFLVEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEYMPLG 156

Query: 166 SLDKHLHDVPPGKKVLDWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYW 225
           SL+ HLHD+ PGK+ LDWNTRMKIA GAAKGLEYLH+K  P VIYRDLKCSNILL + Y+
Sbjct: 157 SLEDHLHDISPGKQPLDWNTRMKIAAGAAKGLEYLHDKTMPPVIYRDLKCSNILLDDDYF 216

Query: 226 PKLSDFGLAKVGPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITG 285
           PKLSDFGLAK+GP GD +HVSTRVMGTYGYCAP+YAMTGQLT K D+YSFGVVLLE+ITG
Sbjct: 217 PKLSDFGLAKLGPVGDKSHVSTRVMGTYGYCAPEYAMTGQLTLKSDVYSFGVVLLEIITG 276

Query: 286 RKAIDHTRPPKELSLVAWVSYLTK 308
           RKAID +R   E +LVAW   L K
Sbjct: 277 RKAIDSSRSTGEQNLVAWARPLFK 297

BLAST of HG10001415 vs. ExPASy TrEMBL
Match: A0A6J1JCJ0 (probable serine/threonine-protein kinase PBL5 OS=Cucurbita maxima OX=3661 GN=LOC111484493 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 7.5e-143
Identity = 252/306 (82.35%), Postives = 275/306 (89.87%), Query Frame = 0

Query: 1   MGCFSHIKKQRKRL-LRSNVQSIQPASPVKADLKVKRGEEIKEVVYENEQLASNINEEVS 60
           MGCF H KKQRK+  + S + S++    V+ADLKV+  EE+KE   EN+QLASNIN EVS
Sbjct: 1   MGCFRHSKKQRKKFEINSPISSVK----VEADLKVETREEVKEQTRENDQLASNINREVS 60

Query: 61  SEVKTKEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQLDRNGV 120
            +VKT EEIE FTF EL  AT+NFKSDCFLGEGGFGKVYKGHL RI+++VAIKQLDRNG+
Sbjct: 61  GKVKTNEEIEKFTFHELVEATRNFKSDCFLGEGGFGKVYKGHLIRIDQVVAIKQLDRNGL 120

Query: 121 QGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDVPPGKKVL 180
           QGVREFVVEV+TLSLA+HPNLVKL+GYCA+ D+RLLVYEFMPLGSLDKHLHD+PPGK VL
Sbjct: 121 QGVREFVVEVLTLSLADHPNLVKLLGYCAERDERLLVYEFMPLGSLDKHLHDLPPGKNVL 180

Query: 181 DWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSGD 240
           DWNTRMKIAVGAA+GLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSGD
Sbjct: 181 DWNTRMKIAVGAARGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSGD 240

Query: 241 NTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPPKELSLV 300
           NTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRK ID+TRPPKELSLV
Sbjct: 241 NTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKVIDYTRPPKELSLV 300

Query: 301 AWVSYL 306
            W   L
Sbjct: 301 TWAKPL 302

BLAST of HG10001415 vs. ExPASy TrEMBL
Match: A0A0A0KC79 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G301000 PE=3 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.7e-142
Identity = 259/308 (84.09%), Postives = 274/308 (88.96%), Query Frame = 0

Query: 1   MGCFSHIKKQRKRLLRSNVQSIQPAS-----PVKADLKVKRGEEIKEVVYENE-QLASNI 60
           MGCFSHI+KQ+KRL  S ++SIQPAS      VK DLKVK  EE KEVVYENE +L SNI
Sbjct: 1   MGCFSHIRKQKKRLSGSKLESIQPASSGNAVKVKRDLKVKTREEAKEVVYENEHRLGSNI 60

Query: 61  NEEVSSEVKT-KEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQ 120
            +EV  E KT  EE++ FTF ELA AT+NFKSDCFLGEGGFGKVYKG+L RINKLVAIKQ
Sbjct: 61  KKEVCGEAKTSNEEVKTFTFHELAEATRNFKSDCFLGEGGFGKVYKGYLARINKLVAIKQ 120

Query: 121 LDRNGVQGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDVP 180
           LD +  QG+REF+VEVVTLSLAN PNLVKLIGYC +GDQRLLVYEFMPLGSLDKHLHDVP
Sbjct: 121 LDWSRGQGIREFMVEVVTLSLANDPNLVKLIGYCVEGDQRLLVYEFMPLGSLDKHLHDVP 180

Query: 181 PGKKVLDWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAK 240
            G KVLDWNTRMKIAVGAAKGLEYLHNKM+PAVIYRDLKCSNILLGE YWPKLSDFGLAK
Sbjct: 181 AGNKVLDWNTRMKIAVGAAKGLEYLHNKMKPAVIYRDLKCSNILLGEDYWPKLSDFGLAK 240

Query: 241 VGPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPP 300
           VGPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRP 
Sbjct: 241 VGPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPS 300

Query: 301 KELSLVAW 302
           KELSLVAW
Sbjct: 301 KELSLVAW 308

BLAST of HG10001415 vs. ExPASy TrEMBL
Match: A0A1S4E4E4 (probable serine/threonine-protein kinase RLCKVII OS=Cucumis melo OX=3656 GN=LOC103501897 PE=3 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 5.4e-141
Identity = 257/307 (83.71%), Postives = 271/307 (88.27%), Query Frame = 0

Query: 1   MGCFSHIKKQRKRLLRSNVQSIQPAS-----PVKADLKVKRGEEIKEVVYENE-QLASNI 60
           MGCFSHIKKQRKRLL S +QSIQPAS      V+ +LKVK  EE KEVV EN+ +L SNI
Sbjct: 1   MGCFSHIKKQRKRLLGSKLQSIQPASSANEVKVRRELKVKTREEAKEVVCENDHRLGSNI 60

Query: 61  NEEVSSEVKTKEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQL 120
           N+ V SE K KEE++ FTF ELA AT NFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQL
Sbjct: 61  NKGVCSEAKAKEEVKTFTFHELAEATGNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQL 120

Query: 121 DRNGVQGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDVPP 180
           D +  QG+REF+VEVVTLSLAN PNLVKLIGYC +GDQRLLVYEFMPLGSLD+HLHDVP 
Sbjct: 121 DWSRGQGIREFMVEVVTLSLANDPNLVKLIGYCVEGDQRLLVYEFMPLGSLDRHLHDVPA 180

Query: 181 GKKVLDWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKV 240
           G+KVLDWNTRM IAVGAAKGLEYLH KM PAVIYRDLKCSNILLGEGYW KLSDFGLAKV
Sbjct: 181 GEKVLDWNTRMNIAVGAAKGLEYLHTKMSPAVIYRDLKCSNILLGEGYWAKLSDFGLAKV 240

Query: 241 GPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPPK 300
           GPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFK DIYSFGVVLLELITGRKAIDHTRP  
Sbjct: 241 GPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELITGRKAIDHTRPSN 300

Query: 301 ELSLVAW 302
           ELSLVAW
Sbjct: 301 ELSLVAW 307

BLAST of HG10001415 vs. ExPASy TrEMBL
Match: A0A6J1FSM6 (probable serine/threonine-protein kinase PBL5 OS=Cucurbita moschata OX=3662 GN=LOC111448108 PE=3 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 1.0e-139
Identity = 249/303 (82.18%), Postives = 268/303 (88.45%), Query Frame = 0

Query: 1   MGCFSHIKKQRKRLLRSNVQSIQPASPVK--ADLKVKRGEEIKEVVYENEQLASNINEEV 60
           MGCF H KKQRK+       S  P S VK  ADLKV+  EE+KE   EN+QLASNI  EV
Sbjct: 1   MGCFRHSKKQRKKF-----DSNSPISSVKVEADLKVETREEVKEQTPENDQLASNITREV 60

Query: 61  SSEVKTKEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQLDRNG 120
           SS+ K  EEIE FT+ EL  AT+NFKSDCFLGEGGFGKVYKGHL RI+++VAIKQLDRNG
Sbjct: 61  SSKAKRNEEIEKFTYHELVEATRNFKSDCFLGEGGFGKVYKGHLIRIDQVVAIKQLDRNG 120

Query: 121 VQGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDVPPGKKV 180
           +QGVREFVVEV+TLSLA+HPNLVKL+GYCA+ D+RLLVYEFMPLGSLDKHLHD+PP K V
Sbjct: 121 LQGVREFVVEVLTLSLADHPNLVKLLGYCAERDERLLVYEFMPLGSLDKHLHDLPPDKNV 180

Query: 181 LDWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSG 240
           LDWNTRMKIAVGAA+GLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSG
Sbjct: 181 LDWNTRMKIAVGAARGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSG 240

Query: 241 DNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPPKELSL 300
           DNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRK ID+TRPPKELSL
Sbjct: 241 DNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKVIDYTRPPKELSL 298

Query: 301 VAW 302
           V W
Sbjct: 301 VTW 298

BLAST of HG10001415 vs. ExPASy TrEMBL
Match: A0A6J1CXM8 (probable serine/threonine-protein kinase PBL5 OS=Momordica charantia OX=3673 GN=LOC111015384 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.4e-135
Identity = 248/315 (78.73%), Postives = 266/315 (84.44%), Query Frame = 0

Query: 1   MGCFSHIKKQRKRLLRSNVQSIQP-----ASPVKADLKVKRGEEIKEVVYENEQLASNI- 60
           MGCF H KKQRKR    N+  I P        +KA+L VK GEE  EVV EN+QLAS+  
Sbjct: 1   MGCFGHSKKQRKR-SEINLPCIIPPPSSDTGDLKANLNVKTGEEENEVVCENDQLASDTN 60

Query: 61  -------NEEVSSEVKTKEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRI-N 120
                  N EVS + KT E IE FTF EL  ATKNFKSDCFLGEGGFGKVY+GHL RI N
Sbjct: 61  IEDLDSHNNEVSKKAKTNEGIETFTFNELVVATKNFKSDCFLGEGGFGKVYRGHLGRINN 120

Query: 121 KLVAIKQLDRNGVQGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLD 180
           + VAIKQLDRNG+QGVREF+VEV+TLSLANH NLVKLIGYCA+GDQRLLVYEFMPLGSLD
Sbjct: 121 QAVAIKQLDRNGLQGVREFMVEVLTLSLANHSNLVKLIGYCAEGDQRLLVYEFMPLGSLD 180

Query: 181 KHLHDVPPGKKVLDWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKL 240
           KHLHD PPG+KVLDWNTRMKIA GAA+GLEYLH+KM PAVIYRDLKCSNILLG+GYWPKL
Sbjct: 181 KHLHDAPPGRKVLDWNTRMKIAAGAARGLEYLHDKMTPAVIYRDLKCSNILLGDGYWPKL 240

Query: 241 SDFGLAKVGPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKA 300
           SDFGLAKVGPSGD THVSTRVMGTYGYCAPDYAMTGQLTFK DIYSFGVVLLELITGRKA
Sbjct: 241 SDFGLAKVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELITGRKA 300

Query: 301 IDHTRPPKELSLVAW 302
           IDHTRPP+E+SLVAW
Sbjct: 301 IDHTRPPREMSLVAW 314

BLAST of HG10001415 vs. TAIR 10
Match: AT1G07870.1 (Protein kinase superfamily protein )

HSP 1 Score: 405.2 bits (1040), Expect = 4.7e-113
Identity = 194/255 (76.08%), Postives = 223/255 (87.45%), Query Frame = 0

Query: 53  NINEEVSSEVKTKEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIK 112
           N+N++V     T ++ + FTFQELA AT NF+SDCFLGEGGFGKV+KG + +++++VAIK
Sbjct: 78  NLNDQV-----TGKKAQTFTFQELAEATGNFRSDCFLGEGGFGKVFKGTIEKLDQVVAIK 137

Query: 113 QLDRNGVQGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDV 172
           QLDRNGVQG+REFVVEV+TLSLA+HPNLVKLIG+CA+GDQRLLVYE+MP GSL+ HLH +
Sbjct: 138 QLDRNGVQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMPQGSLEDHLHVL 197

Query: 173 PPGKKVLDWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLA 232
           P GKK LDWNTRMKIA GAA+GLEYLH++M P VIYRDLKCSNILLGE Y PKLSDFGLA
Sbjct: 198 PSGKKPLDWNTRMKIAAGAARGLEYLHDRMTPPVIYRDLKCSNILLGEDYQPKLSDFGLA 257

Query: 233 KVGPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRP 292
           KVGPSGD THVSTRVMGTYGYCAPDYAMTGQLTFK DIYSFGVVLLELITGRKAID+T+ 
Sbjct: 258 KVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELITGRKAIDNTKT 317

Query: 293 PKELSLVAWVSYLTK 308
            K+ +LV W   L K
Sbjct: 318 RKDQNLVGWARPLFK 327

BLAST of HG10001415 vs. TAIR 10
Match: AT1G07870.2 (Protein kinase superfamily protein )

HSP 1 Score: 405.2 bits (1040), Expect = 4.7e-113
Identity = 194/255 (76.08%), Postives = 223/255 (87.45%), Query Frame = 0

Query: 53  NINEEVSSEVKTKEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIK 112
           N+N++V     T ++ + FTFQELA AT NF+SDCFLGEGGFGKV+KG + +++++VAIK
Sbjct: 78  NLNDQV-----TGKKAQTFTFQELAEATGNFRSDCFLGEGGFGKVFKGTIEKLDQVVAIK 137

Query: 113 QLDRNGVQGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDV 172
           QLDRNGVQG+REFVVEV+TLSLA+HPNLVKLIG+CA+GDQRLLVYE+MP GSL+ HLH +
Sbjct: 138 QLDRNGVQGIREFVVEVLTLSLADHPNLVKLIGFCAEGDQRLLVYEYMPQGSLEDHLHVL 197

Query: 173 PPGKKVLDWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLA 232
           P GKK LDWNTRMKIA GAA+GLEYLH++M P VIYRDLKCSNILLGE Y PKLSDFGLA
Sbjct: 198 PSGKKPLDWNTRMKIAAGAARGLEYLHDRMTPPVIYRDLKCSNILLGEDYQPKLSDFGLA 257

Query: 233 KVGPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRP 292
           KVGPSGD THVSTRVMGTYGYCAPDYAMTGQLTFK DIYSFGVVLLELITGRKAID+T+ 
Sbjct: 258 KVGPSGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELITGRKAIDNTKT 317

Query: 293 PKELSLVAWVSYLTK 308
            K+ +LV W   L K
Sbjct: 318 RKDQNLVGWARPLFK 327

BLAST of HG10001415 vs. TAIR 10
Match: AT2G28590.1 (Protein kinase superfamily protein )

HSP 1 Score: 401.4 bits (1030), Expect = 6.8e-112
Identity = 208/323 (64.40%), Postives = 239/323 (73.99%), Query Frame = 0

Query: 1   MGCFSHIKKQRKR--------------LLRSNVQSIQPASPVKADLKVK-RGEEIKEVVY 60
           MGCF    K  KR               L  N    +   P    LKV   G+  KE+V 
Sbjct: 1   MGCFGRTPKSNKRSDTKTTKNNDFTPKKLTVNANRDKLTQPSSDCLKVSICGDVSKEIVT 60

Query: 61  ENEQLASNINE-EVSSEVKTKEEIEAFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRR 120
           + +QLA +  +  V  EV  K + + FTF+EL+ +T NFKSDCFLGEGGFGKVYKG + +
Sbjct: 61  KKDQLALDAKDTNVEDEVIVK-KAQTFTFEELSVSTGNFKSDCFLGEGGFGKVYKGFIEK 120

Query: 121 INKLVAIKQLDRNGVQGVREFVVEVVTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGS 180
           IN++VAIKQLDRNG QG+REFVVEV+TLSLA+HPNLVKLIG+CA+G QRLLVYE+MPLGS
Sbjct: 121 INQVVAIKQLDRNGAQGIREFVVEVLTLSLADHPNLVKLIGFCAEGVQRLLVYEYMPLGS 180

Query: 181 LDKHLHDVPPGKKVLDWNTRMKIAVGAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWP 240
           LD HLHD+P GK  L WNTRMKIA GAA+GLEYLH+ M+P VIYRDLKCSNIL+ EGY  
Sbjct: 181 LDNHLHDLPSGKNPLAWNTRMKIAAGAARGLEYLHDTMKPPVIYRDLKCSNILIDEGYHA 240

Query: 241 KLSDFGLAKVGPSGDNTHVSTRVMGTYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGR 300
           KLSDFGLAKVGP G  THVSTRVMGTYGYCAPDYA+TGQLTFK D+YSFGVVLLELITGR
Sbjct: 241 KLSDFGLAKVGPRGSETHVSTRVMGTYGYCAPDYALTGQLTFKSDVYSFGVVLLELITGR 300

Query: 301 KAIDHTRPPKELSLVAWVSYLTK 308
           KA D+TR     SLV W + L K
Sbjct: 301 KAYDNTRTRNHQSLVEWANPLFK 322

BLAST of HG10001415 vs. TAIR 10
Match: AT5G18610.1 (Protein kinase superfamily protein )

HSP 1 Score: 380.9 bits (977), Expect = 9.5e-106
Identity = 190/298 (63.76%), Postives = 222/298 (74.50%), Query Frame = 0

Query: 17  SNVQSIQPASPVKADLKVKRGEEIKEVVYENEQLASNINEEVSSEVKTKEE-------IE 76
           S+ +       VK +L  K G   +      ++  S    E   E+   +E        +
Sbjct: 10  SSAKDAASKDSVKKELSAKDGSVTQSHHISLDKSKSRRGPEQKKELTAPKEGPTAHIAAQ 69

Query: 77  AFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQLDRNGVQGVREFVVEV 136
            FTF+ELAAATKNF+ +C LGEGGFG+VYKG L    ++VA+KQLDRNG+QG REF+VEV
Sbjct: 70  TFTFRELAAATKNFRPECLLGEGGFGRVYKGRLETTGQIVAVKQLDRNGLQGNREFLVEV 129

Query: 137 VTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDVPPGKKVLDWNTRMKIAV 196
           + LSL +HPNLV LIGYCADGDQRLLVYE+MPLGSL+ HLHD+PP K+ LDW+TRM IA 
Sbjct: 130 LMLSLLHHPNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLHDLPPDKEPLDWSTRMTIAA 189

Query: 197 GAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSGDNTHVSTRVMG 256
           GAAKGLEYLH+K  P VIYRDLK SNILLG+GY PKLSDFGLAK+GP GD THVSTRVMG
Sbjct: 190 GAAKGLEYLHDKANPPVIYRDLKSSNILLGDGYHPKLSDFGLAKLGPVGDKTHVSTRVMG 249

Query: 257 TYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPPKELSLVAWVSYLTK 308
           TYGYCAP+YAMTGQLT K D+YSFGVV LELITGRKAID+ R P E +LVAW   L K
Sbjct: 250 TYGYCAPEYAMTGQLTLKSDVYSFGVVFLELITGRKAIDNARAPGEHNLVAWARPLFK 307

BLAST of HG10001415 vs. TAIR 10
Match: AT5G18610.2 (Protein kinase superfamily protein )

HSP 1 Score: 380.9 bits (977), Expect = 9.5e-106
Identity = 190/298 (63.76%), Postives = 222/298 (74.50%), Query Frame = 0

Query: 17  SNVQSIQPASPVKADLKVKRGEEIKEVVYENEQLASNINEEVSSEVKTKEE-------IE 76
           S+ +       VK +L  K G   +      ++  S    E   E+   +E        +
Sbjct: 10  SSAKDAASKDSVKKELSAKDGSVTQSHHISLDKSKSRRGPEQKKELTAPKEGPTAHIAAQ 69

Query: 77  AFTFQELAAATKNFKSDCFLGEGGFGKVYKGHLRRINKLVAIKQLDRNGVQGVREFVVEV 136
            FTF+ELAAATKNF+ +C LGEGGFG+VYKG L    ++VA+KQLDRNG+QG REF+VEV
Sbjct: 70  TFTFRELAAATKNFRPECLLGEGGFGRVYKGRLETTGQIVAVKQLDRNGLQGNREFLVEV 129

Query: 137 VTLSLANHPNLVKLIGYCADGDQRLLVYEFMPLGSLDKHLHDVPPGKKVLDWNTRMKIAV 196
           + LSL +HPNLV LIGYCADGDQRLLVYE+MPLGSL+ HLHD+PP K+ LDW+TRM IA 
Sbjct: 130 LMLSLLHHPNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLHDLPPDKEPLDWSTRMTIAA 189

Query: 197 GAAKGLEYLHNKMRPAVIYRDLKCSNILLGEGYWPKLSDFGLAKVGPSGDNTHVSTRVMG 256
           GAAKGLEYLH+K  P VIYRDLK SNILLG+GY PKLSDFGLAK+GP GD THVSTRVMG
Sbjct: 190 GAAKGLEYLHDKANPPVIYRDLKSSNILLGDGYHPKLSDFGLAKLGPVGDKTHVSTRVMG 249

Query: 257 TYGYCAPDYAMTGQLTFKCDIYSFGVVLLELITGRKAIDHTRPPKELSLVAWVSYLTK 308
           TYGYCAP+YAMTGQLT K D+YSFGVV LELITGRKAID+ R P E +LVAW   L K
Sbjct: 250 TYGYCAPEYAMTGQLTLKSDVYSFGVVFLELITGRKAIDNARAPGEHNLVAWARPLFK 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900307.13.1e-15189.11probable serine/threonine-protein kinase PBL5 [Benincasa hispida] >XP_038900308.... [more]
XP_022986891.11.6e-14282.35probable serine/threonine-protein kinase PBL5 [Cucurbita maxima][more]
XP_004148248.13.5e-14284.09probable serine/threonine-protein kinase PBL5 [Cucumis sativus] >KGN47330.1 hypo... [more]
XP_023511984.11.0e-14182.72probable serine/threonine-protein kinase PBL5 [Cucurbita pepo subsp. pepo][more]
XP_016903103.11.1e-14083.71PREDICTED: probable serine/threonine-protein kinase RLCKVII [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9LQQ86.6e-11276.08Probable serine/threonine-protein kinase PBL5 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SIB69.5e-11164.40Probable serine/threonine-protein kinase PBL6 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q6I5Q61.6e-10577.22Receptor-like cytoplasmic kinase 185 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q1PDV61.3e-10463.76Serine/threonine-protein kinase PBL27 OS=Arabidopsis thaliana OX=3702 GN=PBL27 P... [more]
Q0WRY51.2e-10268.56Probable serine/threonine-protein kinase PBL7 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1JCJ07.5e-14382.35probable serine/threonine-protein kinase PBL5 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A0A0KC791.7e-14284.09Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G301... [more]
A0A1S4E4E45.4e-14183.71probable serine/threonine-protein kinase RLCKVII OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1FSM61.0e-13982.18probable serine/threonine-protein kinase PBL5 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1CXM83.4e-13578.73probable serine/threonine-protein kinase PBL5 OS=Momordica charantia OX=3673 GN=... [more]
Match NameE-valueIdentityDescription
AT1G07870.14.7e-11376.08Protein kinase superfamily protein [more]
AT1G07870.24.7e-11376.08Protein kinase superfamily protein [more]
AT2G28590.16.8e-11264.40Protein kinase superfamily protein [more]
AT5G18610.19.5e-10663.76Protein kinase superfamily protein [more]
AT5G18610.29.5e-10663.76Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 83..310
e-value: 2.4E-27
score: 106.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 88..289
e-value: 2.3E-42
score: 145.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 83..311
score: 34.887672
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 52..159
e-value: 2.5E-32
score: 113.1
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 160..309
e-value: 1.1E-46
score: 160.7
NoneNo IPR availablePANTHERPTHR47985:SF41SERINE/THREONINE-PROTEIN KINASE PBL5-RELATEDcoord: 5..306
NoneNo IPR availablePANTHERPTHR47985OS07G0668900 PROTEINcoord: 5..306
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 89..112
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 206..218
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 68..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10001415.1HG10001415.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity