HG10001396 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10001396
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	(R)-specific enoyl-CoA hydratase
Location	Chr09: 16699198 .. 16699928 (+)
RNA-Seq Expression	HG10001396
Synteny	HG10001396

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTAAGTAGAGGTTTATTCTCAATTTCTTTGAAATGCTTCTCTTCATCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGAGCCATTCCAGAATATTCACCAGTGAAGATGTTTTGGAATACTCCAAAGTGAGTCATGACTCAAATCCTCTGCATTTTGATTCTGAATTAGCTCGACGTGCTGGATTCGACGGTCGTCTTGTTCACGGGATGCTTGTTGCTTCAATGTTCCCTCACATCATTTCCTCTCATTTTGTAAGTCTAGCCAGTTTTCATTTCTTACACTTGGGGGAATGAGAAATGGCACCTGAAACTTTCTTACAACTGATTTAATGCTTGTTTAAGATCTTTTAATGTATGGCCTAATCTTTGAAGATGCTTTGATCTATAATGGTAATGTGTTGAGGATGATTGTAGCCCGGAGCCATCTACGTGTCGCAAAGCTTGAATTTTAAGTTGCCAGTCTATGTTGGAGAGAAGATCGTTGGTCAGGTAGAAGCAATCGATTTGAGAGAAAATAAGAAGAGATACCTGTAAGCTTGTTCTTGGGCAAATCTTTACCTTCTCAAATTCAAATTTCAAGTTGTTATTTCATTGAGTTGGGTAGTTTCCAAATGTGTTTCAGGGCAAAATTCAAAATGAAATGCTTGAGGAATGGCGACGAACTTGTTCTCGAAGGTGAGGCAAGGGCCATCATACCATCCGTTTTTCATTCCACAAACGGGTAG

mRNA sequence

ATGCTAAGTAGAGGTTTATTCTCAATTTCTTTGAAATGCTTCTCTTCATCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGAGCCATTCCAGAATATTCACCAGTGAAGATGTTTTGGAATACTCCAAAGTGAGTCATGACTCAAATCCTCTGCATTTTGATTCTGAATTAGCTCGACGTGCTGGATTCGACGGTCGTCTTGTTCACGGGATGCTTGTTGCTTCAATGTTCCCTCACATCATTTCCTCTCATTTTCCCGGAGCCATCTACGTGTCGCAAAGCTTGAATTTTAAGTTGCCAGTCTATGTTGGAGAGAAGATCGTTGGTCAGGTAGAAGCAATCGATTTGAGAGAAAATAAGAAGAGATACCTGGCAAAATTCAAAATGAAATGCTTGAGGAATGGCGACGAACTTGTTCTCGAAGGTGAGGCAAGGGCCATCATACCATCCGTTTTTCATTCCACAAACGGGTAG

Coding sequence (CDS)

ATGCTAAGTAGAGGTTTATTCTCAATTTCTTTGAAATGCTTCTCTTCATCAACTTCTAATGTGCTCAAAGTTGGAGATATCTTGAGCCATTCCAGAATATTCACCAGTGAAGATGTTTTGGAATACTCCAAAGTGAGTCATGACTCAAATCCTCTGCATTTTGATTCTGAATTAGCTCGACGTGCTGGATTCGACGGTCGTCTTGTTCACGGGATGCTTGTTGCTTCAATGTTCCCTCACATCATTTCCTCTCATTTTCCCGGAGCCATCTACGTGTCGCAAAGCTTGAATTTTAAGTTGCCAGTCTATGTTGGAGAGAAGATCGTTGGTCAGGTAGAAGCAATCGATTTGAGAGAAAATAAGAAGAGATACCTGGCAAAATTCAAAATGAAATGCTTGAGGAATGGCGACGAACTTGTTCTCGAAGGTGAGGCAAGGGCCATCATACCATCCGTTTTTCATTCCACAAACGGGTAG

Protein sequence

MLSRGLFSISLKCFSSSTSNVLKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFDSELARRAGFDGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIVGQVEAIDLRENKKRYLAKFKMKCLRNGDELVLEGEARAIIPSVFHSTNG

Homology

[Close]

BLAST of HG10001396 vs. NCBI nr
Match: XP_038901209.1 ((R)-specific enoyl-CoA hydratase [Benincasa hispida])

HSP 1 Score: 285.0 bits (728), Expect = 3.9e-73
Identity = 146/162 (90.12%), Postives = 153/162 (94.44%), Query Frame = 0


Query: 1   MLSRGLFSI----SLKCFSSSTSNVLKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFDS 60
           MLSRGLFS+    SLKCFSSSTSNVLKVGDILS++RIFTS+DVLEYSKVSHDSNPLHFDS
Sbjct: 1   MLSRGLFSMHISTSLKCFSSSTSNVLKVGDILSYTRIFTSKDVLEYSKVSHDSNPLHFDS 60

Query: 61  ELARRAGFDGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIVGQVEAID 120
           ELARRAGF+GRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGE IVGQVEAID
Sbjct: 61  ELARRAGFNGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGENIVGQVEAID 120

Query: 121 LRENKKRYLAKFKMKCLRNGDELVLEGEARAIIPSVFHSTNG 159
           LRENKKRYLAKF  KC+RNG E+VLEGEARAIIPS FHSTNG
Sbjct: 121 LRENKKRYLAKFITKCMRNGHEVVLEGEARAIIPSDFHSTNG 162

[Close]

BLAST of HG10001396 vs. NCBI nr
Match: XP_008463911.1 (PREDICTED: (R)-specific enoyl-CoA hydratase [Cucumis melo])

HSP 1 Score: 283.1 bits (723), Expect = 1.5e-72
Identity = 144/161 (89.44%), Postives = 152/161 (94.41%), Query Frame = 0


Query: 1   MLSRGLFSI----SLKCFSSSTSNVLKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFDS 60
           MLSRGLFS     SLKCFSSSTSNVLKVGDILS++RIFTSEDVLEYSKVSHDSNPLHFD 
Sbjct: 45  MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 104

Query: 61  ELARRAGFDGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIVGQVEAID 120
           ELARRAGF+G LVHG+LVASMFPHIISSH+PGAIYVSQSLNFKLPVYVGEKIVGQVEAI+
Sbjct: 105 ELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 164

Query: 121 LRENKKRYLAKFKMKCLRNGDELVLEGEARAIIPSVFHSTN 158
           LRENKKRYLAKFK KCLRNGDELVLEGEARAI+P+ FHSTN
Sbjct: 165 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 205

[Close]

BLAST of HG10001396 vs. NCBI nr
Match: KAA0035311.1 ((R)-specific enoyl-CoA hydratase [Cucumis melo var. makuwa] >TYK14325.1 (R)-specific enoyl-CoA hydratase [Cucumis melo var. makuwa])

HSP 1 Score: 283.1 bits (723), Expect = 1.5e-72
Identity = 144/161 (89.44%), Postives = 152/161 (94.41%), Query Frame = 0


Query: 1   MLSRGLFSI----SLKCFSSSTSNVLKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFDS 60
           MLSRGLFS     SLKCFSSSTSNVLKVGDILS++RIFTSEDVLEYSKVSHDSNPLHFD 
Sbjct: 1   MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 60

Query: 61  ELARRAGFDGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIVGQVEAID 120
           ELARRAGF+G LVHG+LVASMFPHIISSH+PGAIYVSQSLNFKLPVYVGEKIVGQVEAI+
Sbjct: 61  ELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 120

Query: 121 LRENKKRYLAKFKMKCLRNGDELVLEGEARAIIPSVFHSTN 158
           LRENKKRYLAKFK KCLRNGDELVLEGEARAI+P+ FHSTN
Sbjct: 121 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 161

[Close]

BLAST of HG10001396 vs. NCBI nr
Match: XP_004149553.1 (uncharacterized protein LOC101214584 [Cucumis sativus] >KGN47303.1 hypothetical protein Csa_022963 [Cucumis sativus])

HSP 1 Score: 276.9 bits (707), Expect = 1.1e-70
Identity = 141/161 (87.58%), Postives = 152/161 (94.41%), Query Frame = 0


Query: 1   MLSRGLFSI----SLKCFSSSTSNVLKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFDS 60
           MLS+GLFS     SLKCFSSSTS+VLKVGDILS++RIFTSEDVLEYSKVSHDSNPLHFDS
Sbjct: 1   MLSKGLFSTYISSSLKCFSSSTSSVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDS 60

Query: 61  ELARRAGFDGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIVGQVEAID 120
           +LA+RAGF+G LVHG+LVASMFPHIISSH+PGAIYVSQSLNFKLPVYVGEKIVGQVEAI+
Sbjct: 61  KLAQRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 120

Query: 121 LRENKKRYLAKFKMKCLRNGDELVLEGEARAIIPSVFHSTN 158
           LRENKKRYLAKFK KCLRNGDELVLEGEARAI+P  FHSTN
Sbjct: 121 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPVDFHSTN 161

[Close]

BLAST of HG10001396 vs. NCBI nr
Match: XP_023512018.1 (uncharacterized protein LOC111776855 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 267.7 bits (683), Expect = 6.4e-68
Identity = 139/163 (85.28%), Postives = 147/163 (90.18%), Query Frame = 0


Query: 1   MLSRG-----LFSISLKCFSSSTSNVLKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLSRG       S SLKCFSSSTSNVLKVGDILSH+RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLVSSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SELARRAGFDGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 120
           S LARRAGFD RLVHGMLVASMFPH+ISSHFPGAIYVSQSLNFKLPVYVGE I+ +VEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIMVRVEAI 120

Query: 121 DLRENKKRYLAKFKMKCLRNGDELVLEGEARAIIPSVFHSTNG 159
           DLRE KKRYLAKF+ KCLRNGDELVL+GEARAI+PS F ST+G
Sbjct: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSGFCSTDG 163

[Close]

BLAST of HG10001396 vs. ExPASy Swiss-Prot
Match: O32472 ((R)-specific enoyl-CoA hydratase OS=Aeromonas caviae OX=648 GN=phaJ PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 3.7e-18
Identity = 50/131 (38.17%), Postives = 76/131 (58.02%), Query Frame = 0


Query: 22  LKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFDSELARRAGFDGRLVHGMLVASMFPHI 81
           L+VG     S+ F + +V  ++ +S D NPLH D   A    F+  +VHGML+AS+F  +
Sbjct: 6   LEVGQKARLSKRFGAAEVAAFAALSEDFNPLHLDPAFAATTAFERPIVHGMLLASLFSGL 65

Query: 82  ISSHFP--GAIYVSQSLNFKLPVYVGEKIVGQVEAIDLRENKKRYLAKFKMKCLRNGDEL 141
           +    P  G+IY+ QSL+FKLPV+VG+++  +VE   LRE+K   +A    +    G  L
Sbjct: 66  LGQQLPGKGSIYLGQSLSFKLPVFVGDEVTAEVEVTALREDKP--IATLTTRIFTQGGAL 125

Query: 142 VLEGEARAIIP 151
            + GEA   +P
Sbjct: 126 AVTGEAVVKLP 134

[Close]

BLAST of HG10001396 vs. ExPASy Swiss-Prot
Match: C5AZ74 (3-hydroxybutyryl-CoA dehydratase OS=Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1) OX=272630 GN=croR PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 5.0e-15
Identity = 44/131 (33.59%), Postives = 75/131 (57.25%), Query Frame = 0


Query: 22  LKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFDSELARRAGFDGRLVHGMLVASMFPHI 81
           L+VG      +  +S DV+ +++++ D NP+H     A R  F  R+ HG+  A +   +
Sbjct: 13  LEVGLTEILKKEISSSDVVGFAEITGDRNPIHLSEHFAARTPFGTRIAHGLYTAGLISAV 72

Query: 82  ISSHF--PGAIYVSQSLNFKLPVYVGEKIVGQVEAIDLRENKKRYLAKFKMKCLRNGDEL 141
           + +    PGA+Y+SQ+LNF+ PV +G+ +  +VE  +L   ++R  A+    C   GDE+
Sbjct: 73  LGTRLPGPGAVYISQTLNFRAPVRIGDIVEVKVEVAELIPERRR--ARLACTC-SVGDEV 132

Query: 142 VLEGEARAIIP 151
           VL+GEA   +P
Sbjct: 133 VLDGEALVKVP 140

[Close]

BLAST of HG10001396 vs. ExPASy Swiss-Prot
Match: P86397 (Hydroxyacyl-thioester dehydratase type 2, mitochondrial OS=Homo sapiens OX=9606 GN=HTD2 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 8.5e-15
Identity = 41/137 (29.93%), Postives = 74/137 (54.01%), Query Frame = 0


Query: 22  LKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFDSELARRAGFDGRLVHGMLVASMFPHI 81
           +KVGD     R FT  DV  +S+++ D NPLH + + A+   F   +VHG+L+  +   +
Sbjct: 36  IKVGDRAELRRAFTQTDVATFSELTGDVNPLHLNEDFAKHTKFGNTIVHGVLINGLISAL 95

Query: 82  ISSHF--PGAIYVSQSLNFKLPVYVGEKIVGQVEAIDLRENKKRYLAKFKMKC-LRNGDE 141
           + +    PG +++SQ ++F  P+Y+GE ++   E   L    KR++A   + C +    +
Sbjct: 96  LGTKMPGPGCVFLSQEISFPAPLYIGEVVLASAEVKKL----KRFIAIIAVSCSVIESKK 155

Query: 142 LVLEGEARAIIPSVFHS 156
            V+EG  + ++P    S
Sbjct: 156 TVMEGWVKVMVPEASKS 168

[Close]

BLAST of HG10001396 vs. ExPASy Swiss-Prot
Match: Q2RQ36 ((R)-specific enoyl-CoA hydratase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=phaJ PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 4.2e-14
Identity = 44/132 (33.33%), Postives = 71/132 (53.79%), Query Frame = 0


Query: 22  LKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFDSELARRAGFDGRLVHGMLVASMFPHI 81
           +K G   S ++  +  D+  ++ +S D+NP H + + A+   F  R+ HGML A     +
Sbjct: 14  IKEGQSASLAKTISESDIYLFAGLSMDTNPAHVNEDYAQTTVFKTRIAHGMLSAGFISAV 73

Query: 82  ISSHF--PGAIYVSQSLNFKLPVYVGEKIVGQVEAIDLRENKKRYLAKFKMKCLRNGDEL 141
           + +    PGAIYV+QSL FK PV +G+ +   V    L   KK     F+  C   G ++
Sbjct: 74  LGTRLPGPGAIYVNQSLKFKAPVRIGDTVTATVTVTGLVPEKK--FVTFRTTCTVAG-KV 133

Query: 142 VLEGEARAIIPS 152
           V+EGEA  ++P+
Sbjct: 134 VIEGEATVMVPA 142

[Close]

BLAST of HG10001396 vs. ExPASy Swiss-Prot
Match: Q9VXJ0 (Peroxisomal multifunctional enzyme type 2 OS=Drosophila melanogaster OX=7227 GN=Mfe2 PE=1 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.8e-04
Identity = 31/109 (28.44%), Postives = 50/109 (45.87%), Query Frame = 0


Query: 35  TSEDVLEYSKVSHDSNPLHFDSELARRAGFDGRLVHGMLVASMFPHIISSHFPG---AIY 94
           TSED     ++S D NPLH D ++A  AGF   ++HG+         + + F     A++
Sbjct: 483 TSEDQAALYRLSGDKNPLHIDPQMALLAGFKTPILHGLCTLGFSVRAVLAQFADNNPALF 542

Query: 95  VSQSLNFKLPVYVGEKIVGQVEAIDLRENKKRYLAKFKMKCLRNGDELV 141
            +  + F  PV     I GQ   +DL +   R    F+   +  G E++
Sbjct: 543 KAVKVRFSGPV-----IPGQTLRVDLWKQGTR--INFRTVVVETGKEVI 584

[Close]

BLAST of HG10001396 vs. ExPASy TrEMBL
Match: A0A5A7T147 ((R)-specific enoyl-CoA hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00540 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 7.1e-73
Identity = 144/161 (89.44%), Postives = 152/161 (94.41%), Query Frame = 0


Query: 1   MLSRGLFSI----SLKCFSSSTSNVLKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFDS 60
           MLSRGLFS     SLKCFSSSTSNVLKVGDILS++RIFTSEDVLEYSKVSHDSNPLHFD 
Sbjct: 1   MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 60

Query: 61  ELARRAGFDGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIVGQVEAID 120
           ELARRAGF+G LVHG+LVASMFPHIISSH+PGAIYVSQSLNFKLPVYVGEKIVGQVEAI+
Sbjct: 61  ELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 120

Query: 121 LRENKKRYLAKFKMKCLRNGDELVLEGEARAIIPSVFHSTN 158
           LRENKKRYLAKFK KCLRNGDELVLEGEARAI+P+ FHSTN
Sbjct: 121 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 161

[Close]

BLAST of HG10001396 vs. ExPASy TrEMBL
Match: A0A1S3CLU3 ((R)-specific enoyl-CoA hydratase OS=Cucumis melo OX=3656 GN=LOC103501925 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 7.1e-73
Identity = 144/161 (89.44%), Postives = 152/161 (94.41%), Query Frame = 0


Query: 1   MLSRGLFSI----SLKCFSSSTSNVLKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFDS 60
           MLSRGLFS     SLKCFSSSTSNVLKVGDILS++RIFTSEDVLEYSKVSHDSNPLHFD 
Sbjct: 45  MLSRGLFSTHISSSLKCFSSSTSNVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDP 104

Query: 61  ELARRAGFDGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIVGQVEAID 120
           ELARRAGF+G LVHG+LVASMFPHIISSH+PGAIYVSQSLNFKLPVYVGEKIVGQVEAI+
Sbjct: 105 ELARRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 164

Query: 121 LRENKKRYLAKFKMKCLRNGDELVLEGEARAIIPSVFHSTN 158
           LRENKKRYLAKFK KCLRNGDELVLEGEARAI+P+ FHSTN
Sbjct: 165 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPAYFHSTN 205

[Close]

BLAST of HG10001396 vs. ExPASy TrEMBL
Match: A0A0A0KCN3 (MaoC-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G290840 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 5.1e-71
Identity = 141/161 (87.58%), Postives = 152/161 (94.41%), Query Frame = 0


Query: 1   MLSRGLFSI----SLKCFSSSTSNVLKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFDS 60
           MLS+GLFS     SLKCFSSSTS+VLKVGDILS++RIFTSEDVLEYSKVSHDSNPLHFDS
Sbjct: 1   MLSKGLFSTYISSSLKCFSSSTSSVLKVGDILSYNRIFTSEDVLEYSKVSHDSNPLHFDS 60

Query: 61  ELARRAGFDGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIVGQVEAID 120
           +LA+RAGF+G LVHG+LVASMFPHIISSH+PGAIYVSQSLNFKLPVYVGEKIVGQVEAI+
Sbjct: 61  KLAQRAGFNGCLVHGLLVASMFPHIISSHYPGAIYVSQSLNFKLPVYVGEKIVGQVEAIE 120

Query: 121 LRENKKRYLAKFKMKCLRNGDELVLEGEARAIIPSVFHSTN 158
           LRENKKRYLAKFK KCLRNGDELVLEGEARAI+P  FHSTN
Sbjct: 121 LRENKKRYLAKFKTKCLRNGDELVLEGEARAILPVDFHSTN 161

[Close]

BLAST of HG10001396 vs. ExPASy TrEMBL
Match: A0A6J1JDU3 (uncharacterized protein LOC111483634 OS=Cucurbita maxima OX=3661 GN=LOC111483634 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.5e-67
Identity = 138/163 (84.66%), Postives = 146/163 (89.57%), Query Frame = 0


Query: 1   MLSRG-----LFSISLKCFSSSTSNVLKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLSRG       S SLKCFSSSTSNVLKVGDILSH+RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLISSYISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SELARRAGFDGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 120
           S LARRAGFD RLVHGMLVASMFP +ISSHFPGAIYVSQSLNFKLPVYVGE I+ +VEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPRVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 121 DLRENKKRYLAKFKMKCLRNGDELVLEGEARAIIPSVFHSTNG 159
           DLRE KKRYLAKF+ KCLRNGDELVL+GEARAI+PS F ST+G
Sbjct: 121 DLREIKKRYLAKFQTKCLRNGDELVLDGEARAILPSDFCSTDG 163

[Close]

BLAST of HG10001396 vs. ExPASy TrEMBL
Match: A0A6J1FRZ2 (uncharacterized protein LOC111448279 OS=Cucurbita moschata OX=3662 GN=LOC111448279 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.4e-66
Identity = 134/156 (85.90%), Postives = 141/156 (90.38%), Query Frame = 0


Query: 1   MLSRG-----LFSISLKCFSSSTSNVLKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFD 60
           MLSRG       S SLKCFSSSTSNVLKVGDILSH+RIFTSEDVLEYSKVSHDSNPLHFD
Sbjct: 1   MLSRGGLISSCISSSLKCFSSSTSNVLKVGDILSHTRIFTSEDVLEYSKVSHDSNPLHFD 60

Query: 61  SELARRAGFDGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIVGQVEAI 120
           S LARRAGFD RLVHGMLVASMFPH+ISSHFPGAIYVSQSLNFKLPVYVGE I+ +VEAI
Sbjct: 61  SALARRAGFDDRLVHGMLVASMFPHVISSHFPGAIYVSQSLNFKLPVYVGETIIVRVEAI 120

Query: 121 DLRENKKRYLAKFKMKCLRNGDELVLEGEARAIIPS 152
           DLRE KKRYL KF+ KCLRNGDELVL+GEARAI+PS
Sbjct: 121 DLREIKKRYLVKFQTKCLRNGDELVLDGEARAILPS 156

[Close]

BLAST of HG10001396 vs. TAIR 10
Match: AT5G60335.1 (Thioesterase superfamily protein )

HSP 1 Score: 183.7 bits (465), Expect = 1.1e-46
Identity = 90/151 (59.60%), Postives = 123/151 (81.46%), Query Frame = 0


Query: 4   RGLFSISLKCFSS-STSNVLKVGDILSHSRIFTSEDVLEYSKVSHDSNPLHFDSELARRA 63
           RGL  + L+ FSS ++  +LKVGD+L  +R+F+SED+  Y++VSHD NPLHFD E AR+A
Sbjct: 9   RGL--LVLRSFSSVASKTLLKVGDVLRETRVFSSEDIKAYAEVSHDWNPLHFDPESARKA 68

Query: 64  GFDGRLVHGMLVASMFPHIISSHFPGAIYVSQSLNFKLPVYVGEKIVGQVEAIDLRENKK 123
           GF+ RLVHGMLV+SMFP IIS+HFPGA+YVSQSL+F+ PVY+G++I+G V+AI LRE K 
Sbjct: 69  GFENRLVHGMLVSSMFPRIISAHFPGAVYVSQSLHFRSPVYIGDEILGLVQAIALRETKN 128

Query: 124 RYLAKFKMKCLRNGDEL-VLEGEARAIIPSV 153
           +Y+ KF  KC +N +EL V++GEA AI+P++
Sbjct: 129 KYIVKFSTKCFKNHNELVVIDGEATAILPNL 157

The following BLAST results are available for this feature:

BLAST of HG10001396 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901209.1	3.9e-73	90.12	(R)-specific enoyl-CoA hydratase [Benincasa hispida]	[more]
XP_008463911.1	1.5e-72	89.44	PREDICTED: (R)-specific enoyl-CoA hydratase [Cucumis melo]	[more]
KAA0035311.1	1.5e-72	89.44	(R)-specific enoyl-CoA hydratase [Cucumis melo var. makuwa] >TYK14325.1 (R)-spec...	[more]
XP_004149553.1	1.1e-70	87.58	uncharacterized protein LOC101214584 [Cucumis sativus] >KGN47303.1 hypothetical ...	[more]
XP_023512018.1	6.4e-68	85.28	uncharacterized protein LOC111776855 [Cucurbita pepo subsp. pepo]	[more]

BLAST of HG10001396 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O32472	3.7e-18	38.17	(R)-specific enoyl-CoA hydratase OS=Aeromonas caviae OX=648 GN=phaJ PE=1 SV=1	[more]
C5AZ74	5.0e-15	33.59	3-hydroxybutyryl-CoA dehydratase OS=Methylorubrum extorquens (strain ATCC 14718 ...	[more]
P86397	8.5e-15	29.93	Hydroxyacyl-thioester dehydratase type 2, mitochondrial OS=Homo sapiens OX=9606 ...	[more]
Q2RQ36	4.2e-14	33.33	(R)-specific enoyl-CoA hydratase OS=Rhodospirillum rubrum (strain ATCC 11170 / A...	[more]
Q9VXJ0	8.8e-04	28.44	Peroxisomal multifunctional enzyme type 2 OS=Drosophila melanogaster OX=7227 GN=...	[more]

BLAST of HG10001396 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T147	7.1e-73	89.44	(R)-specific enoyl-CoA hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3CLU3	7.1e-73	89.44	(R)-specific enoyl-CoA hydratase OS=Cucumis melo OX=3656 GN=LOC103501925 PE=4 SV...	[more]
A0A0A0KCN3	5.1e-71	87.58	MaoC-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G290840 P...	[more]
A0A6J1JDU3	1.5e-67	84.66	uncharacterized protein LOC111483634 OS=Cucurbita maxima OX=3661 GN=LOC111483634...	[more]
A0A6J1FRZ2	6.4e-66	85.90	uncharacterized protein LOC111448279 OS=Cucurbita moschata OX=3662 GN=LOC1114482...	[more]

BLAST of HG10001396 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60335.1	1.1e-46	59.60	Thioesterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.129.10	Hotdog Thioesterase	coord: 10..149 e-value: 5.9E-37 score: 128.3
None	No IPR available	PANTHER	PTHR43437	HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATED	coord: 10..151
None	No IPR available	PANTHER	PTHR43437:SF3	HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL-RELATED	coord: 10..151
None	No IPR available	CDD	cd03449	R_hydratase	coord: 22..149 e-value: 1.52406E-46 score: 146.151
IPR002539	MaoC-like dehydratase domain	PFAM	PF01575	MaoC_dehydratas	coord: 32..121 e-value: 1.1E-17 score: 63.8
IPR029069	HotDog domain superfamily	SUPERFAMILY	54637	Thioesterase/thiol ester dehydrase-isomerase	coord: 22..150

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10001396.1	HG10001396.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006259	DNA metabolic process
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0045145	single-stranded DNA 5'-3' exodeoxyribonuclease activity