HG10001394 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10001394
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionexonuclease V, chloroplastic
LocationChr09: 16690329 .. 16694439 (+)
RNA-Seq ExpressionHG10001394
SyntenyHG10001394
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGATTCAAGTTCTGGGTTACCGCCATTTAACAACGATGACAATACCCACGAAGGCTTCCCAAAGATCCCGGTTGAGATTGTGAGCGACGAGGAAATGGCCCTAATCGAAGCTGCTTTAGCCGCTGCTGTCAGCACTGCTCGATCTTCAACTTCCGCCATTCATTCCCCTTCTTGTTCCTCTCAGAATCTCTTTAACACCAGGTCTATTCACTCTATTACCCTCCTATCCAAGAGGGGCATCTATTCTTCCCAGCCAGATATTGAGGATCTCGGCAAAATCGCGATTACACAGAAGAAGATCAAAGTCAATGAATCGCTTCTGCAACGTTTTATGAGGAACAAGGCTTTGGCCGTCACTGATATTACTCGCACGGTATGGACTTCTTGTTTCGCTGCTCATTGGTTTTGTCTGTCCGTTGACATGTTAATTTGATTTGATCGGAATAAACTTTGAACTCAATTGGTTTGTTTCTATAAGTATAAGTTCTTTCAAAAACACTTCTATCAGTTTATGTTACACTCGGGACCATTTTTGTGCAGGAAGTGCTTGAACACTTCCAAACAGAGCCTGAAGCTGAAGCCTATTGGTTTTTTGAATTGGGAAAGTTATCTCCATATGTTTGTTTTGTTCTCGTCATTTAAAGAACTCTTGAAGTATCACAAGATGGTATAATATGATTTTTCCCATCTTTTTATGGGGTGGAAAATTTATGTACTTAGCTCCTGACATGGCATCTGCAGGAATGGTGTGAAAAACAGACGGAGCTTTCTCTTCTTTCTGGAGTTCGGAAAAAAACCAAAGCTATGGAAGCAGGTATTGCGCGCCATGCAATGCTTGAAGCGGAGGTGAATTTCTAGTCTTCATGTTGCACTGATTTCATTTGTGCGTGCTCTTTAGTATAGCTGTTCCTTACTTCTAGCATAAAAAATGTCTTCCTAAAAAAGAAAAACAAACAAACAAACAAACAAACTTTTAGTAAGTAGTATCTAAAATGGCTTTGTGACTACATGTTGTGAATTGTGATGCTTGAACCATTTATCACGTATATGTAATATGACCTTGTTAGGACTGAATTTTTCTGTGTTAGTTCCCCCACTTGCATGCCTTTTGATTAAATCTTTAGGATAGTTGTCTAAAATCCTATAGCATTTAGGGGGTGTTTGGAGAGCTGGATTGGAATTAGGATTACTGGAAATTAGAGGAGTGTTGGAAAGTGTGAGTAATCAATGACAGAACGAAAATGAGATTGTGAGGCATGAAAACGATGAGAAAGATGATTGGATTGAAAATTGTCGGAACGAAATAAGTTTTGTATTACAAATTGGACCCAAAACGCTCAATCCAAACATGAATTTTGAATTGGATTACACTACATTCTCATCCCATTATGGGCCATCAAACAGCCCCTTAGTGTCAAGTATCTTGTTATCAAGACCTGAGAATATTAGTTTTGAGTAATCTTAGAGAATGTAAATGTTAGGTTGTATCATCATGAACAGAACTAATTAGTAATCGTTATATTCTCCTCTTTACCACCTTTAATTTGTACTCTCTTTGAATCCTTGTAGGTTGTTAAAAAGGTCAAAGTTCAGGTGAAATCGAATGAAGATAGATGGGCTTTGAAGCTTCTTAATTTCATATTTGGTGTAAAGCAACTAGTGCTTGAAGGACTGACTCGTGAACTGCCTGTGTAAGTCCCAATTTTTGTGTCCGTTTCTCCCAATTAATATTGTTTGTCCATCAGCATACTTTCTAGTTACACTCATAAATAGTTATGCCTTGTGGGGGACATTAAAGCTACTGCCTTGTTTAATTCCCCTGAATGAATAGGAGAATGATTTGTTTGATGAAACTAGTTTAGGGGAATGATTTGTTTGATGAAACAAGTTCTTTTTCTGCTCTTCTTATGCAAGTTTCTTGAAATATCAATTACTGACGTTATTCTTAGTATTGCACTATACATTATTCTTATGAACGAAGCGGGAAATGCTTCAATTTTAGCATCATTAACTTAGGACGTTAATGTGATCTTTCAAATTAGTTACATCGGTTAGAAAATGAGTTGAGTGTTATGTGAAAGTAATCTGGTGCTTGCACTCTTACAGCCTATCGTATGGCAGTTGTTTCCCTGTTTTGAGATGGGTATTTTACGTTTTTCCATGATCTGATTAAAAAAATTATTCTCTTTCGCTTCCATTGGTTTTCAGTTTTCAGTTGAAAACTGCTATTGTTCTCTTTTCTCGTACACACGACTTATAGCTATTATTAGGATTATTTCTCTTTCTTGGAATTTTCCACTTCTCTCAATCTCACAGAAGCTATGTTTGCACTGGGGTCAGAATGGGCTTGGTCGACGGTGTATGGATTGTTGGTGTGATTGATGAGATTCAGATGTTGGAGATTAACACCAGTAGAATACCAATGTTGATAGACACAAAAACCCGTGTGCGGGATACAATTCCTGCTGAACCACAAAGAAGAAATGGAAAGTATGTTATTATAGTTGTTTTCTTTAAGCCTTAAGATTATTGTTCAATATCCATCTCTTGATTGCAAAGGATACTGACTTCTACACTTACTATTTTTTCAGGCTTCAATTAATGTGTTATAAGTTCTTATTGGACAGTCTAATTTCTGATGGCTTCTCTATCAGACAATTTTTTGATTTCTTTTCTTTGAATCCTCATTCCACCTTGTCTGAGGAAATTGCGGACAGTACTACTTCATTTGGTTTCACTGCAAAGGTAGAGCTTTTAACTTCTGCTTGTTTTTTCACAATAATAATAACGATGATGCAAAAATGATATACTAACTATACATTTTCAAAATAGGAAAAAAGAAAAAGGAAAAAAGAAAGATGATCTCACCAGTTCCTTCAAATATTTTACATAAAGTAACTACTATAGGTTGACCTTGTGGTCAATAAAGATAAAGATCATGTAAATAATAAAGAGTCTAGAAGGAATGAGTTCATGTCATGGTGAGCCATCTACCTAAAATTTAAGAGGTTGTTTGGGAGACTGGAATGTAATCAAAATTACTGAAAATCAAAAGAGCGTCGGAATGCGTGAGGTATCGAGGGCGGAATAGAAATGGAACAGAAATGAGATTGTGAAACGTGAACATGGTGGGAAAGAGGGTTTGGTTAAAAACTATTGGAACAAAATGAGTTTAATATTACAAATCGGGCCCAAAACGTTCAATTCAAACATAGGTTTTGGATTACATTATAATCTAAACTCATTCCATTACAGGCCCCTCAAACTCTCCCTAATATTCTACGTGTTTCCTTGTCAATTAAGTGTAGTAAGGGTTGGTGGTTCTTCGTGTGAGAATAGTTAAGGTGTTCGCAAAGTGGCTTGAACATACAATTAATAATTATGTGTATATTTTACATAAACGGGAGCTTACTTTTTCAAGCTCTCGACAGAGTAGATTTTCTTTGGGAAGTTTGAGACAGTAGTGTCAACATAATATTAGGTAGTTCTGGAAGACTAACCCTATGCAAATTATTTGGTTTTGTCTTTTTTTATCTTGTTTATACAGACTCTTGATGATGTAATAAGATACTACATGAATTGTTGTAGCATGCTTCCTCCTACTCACAATCAACTCTTACTAAGGTACATTACATTTATACAATAAAGGTTCTGATTTTTTCAATGTTTGTGCTTGATTTGCAATAGTTTTATATGTTATATTGGATTTTGAACTGAACCCTGTTTCTTATATGTTGAACTTTTATTAACCCTGTTTTGTATACTTCACCTTTTCTTCTAGCTCGAACCGAAGCTCGTGCTTTGTCATTTGAAAACTGAAACTCTGGTATTTGTTTCTGAGAAATATTGCAGTTCTGATAAGATATGGTTAATTGTTATGGTGTAGATATGAATCACAAAAGGATCAGTCTATTATTGTTGAAGATGAATTTGCATATGATCATGGTTGGCTCAATAGTCAACTTGAGACCTCATTACAGGTCTGGTGTGGACAGCGAGAACCCGAATGTACTCCTCAGGAAGAACGATGGAAATGTCGACATTGTCAATTTGCTTCTGACTGCCCTGCGAACGCTAACCTCAACGCTACTCGAAAGCCCGAGTAG

mRNA sequence

ATGGCGGATTCAAGTTCTGGGTTACCGCCATTTAACAACGATGACAATACCCACGAAGGCTTCCCAAAGATCCCGGTTGAGATTGTGAGCGACGAGGAAATGGCCCTAATCGAAGCTGCTTTAGCCGCTGCTGTCAGCACTGCTCGATCTTCAACTTCCGCCATTCATTCCCCTTCTTGTTCCTCTCAGAATCTCTTTAACACCAGGTCTATTCACTCTATTACCCTCCTATCCAAGAGGGGCATCTATTCTTCCCAGCCAGATATTGAGGATCTCGGCAAAATCGCGATTACACAGAAGAAGATCAAAGTCAATGAATCGCTTCTGCAACGTTTTATGAGGAACAAGGCTTTGGCCGTCACTGATATTACTCGCACGGAATGGTGTGAAAAACAGACGGAGCTTTCTCTTCTTTCTGGAGTTCGGAAAAAAACCAAAGCTATGGAAGCAGGTATTGCGCGCCATGCAATGCTTGAAGCGGAGGTTGTTAAAAAGGTCAAAGTTCAGGTGAAATCGAATGAAGATAGATGGGCTTTGAAGCTTCTTAATTTCATATTTGGTGTAAAGCAACTAGTGCTTGAAGGACTGACTCGTGAACTGCCTGTAAGCTATGTTTGCACTGGGGTCAGAATGGGCTTGGTCGACGGTGTATGGATTGTTGGTGTGATTGATGAGATTCAGATGTTGGAGATTAACACCAGTAGAATACCAATGTTGATAGACACAAAAACCCGTGTGCGGGATACAATTCCTGCTGAACCACAAAGAAGAAATGGAAAGCTTCAATTAATGTGTTATAAGTTCTTATTGGACAGTCTAATTTCTGATGGCTTCTCTATCAGACAATTTTTTGATTTCTTTTCTTTGAATCCTCATTCCACCTTGTCTGAGGAAATTGCGGACAGTACTACTTCATTTGGTTTCACTGCAAAGACTCTTGATGATGTAATAAGATACTACATGAATTGTTGTAGCATGCTTCCTCCTACTCACAATCAACTCTTACTAAGATATGAATCACAAAAGGATCAGTCTATTATTGTTGAAGATGAATTTGCATATGATCATGGTTGGCTCAATAGTCAACTTGAGACCTCATTACAGGTCTGGTGTGGACAGCGAGAACCCGAATGTACTCCTCAGGAAGAACGATGGAAATGTCGACATTGTCAATTTGCTTCTGACTGCCCTGCGAACGCTAACCTCAACGCTACTCGAAAGCCCGAGTAG

Coding sequence (CDS)

ATGGCGGATTCAAGTTCTGGGTTACCGCCATTTAACAACGATGACAATACCCACGAAGGCTTCCCAAAGATCCCGGTTGAGATTGTGAGCGACGAGGAAATGGCCCTAATCGAAGCTGCTTTAGCCGCTGCTGTCAGCACTGCTCGATCTTCAACTTCCGCCATTCATTCCCCTTCTTGTTCCTCTCAGAATCTCTTTAACACCAGGTCTATTCACTCTATTACCCTCCTATCCAAGAGGGGCATCTATTCTTCCCAGCCAGATATTGAGGATCTCGGCAAAATCGCGATTACACAGAAGAAGATCAAAGTCAATGAATCGCTTCTGCAACGTTTTATGAGGAACAAGGCTTTGGCCGTCACTGATATTACTCGCACGGAATGGTGTGAAAAACAGACGGAGCTTTCTCTTCTTTCTGGAGTTCGGAAAAAAACCAAAGCTATGGAAGCAGGTATTGCGCGCCATGCAATGCTTGAAGCGGAGGTTGTTAAAAAGGTCAAAGTTCAGGTGAAATCGAATGAAGATAGATGGGCTTTGAAGCTTCTTAATTTCATATTTGGTGTAAAGCAACTAGTGCTTGAAGGACTGACTCGTGAACTGCCTGTAAGCTATGTTTGCACTGGGGTCAGAATGGGCTTGGTCGACGGTGTATGGATTGTTGGTGTGATTGATGAGATTCAGATGTTGGAGATTAACACCAGTAGAATACCAATGTTGATAGACACAAAAACCCGTGTGCGGGATACAATTCCTGCTGAACCACAAAGAAGAAATGGAAAGCTTCAATTAATGTGTTATAAGTTCTTATTGGACAGTCTAATTTCTGATGGCTTCTCTATCAGACAATTTTTTGATTTCTTTTCTTTGAATCCTCATTCCACCTTGTCTGAGGAAATTGCGGACAGTACTACTTCATTTGGTTTCACTGCAAAGACTCTTGATGATGTAATAAGATACTACATGAATTGTTGTAGCATGCTTCCTCCTACTCACAATCAACTCTTACTAAGATATGAATCACAAAAGGATCAGTCTATTATTGTTGAAGATGAATTTGCATATGATCATGGTTGGCTCAATAGTCAACTTGAGACCTCATTACAGGTCTGGTGTGGACAGCGAGAACCCGAATGTACTCCTCAGGAAGAACGATGGAAATGTCGACATTGTCAATTTGCTTCTGACTGCCCTGCGAACGCTAACCTCAACGCTACTCGAAAGCCCGAGTAG

Protein sequence

MADSSSGLPPFNNDDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPSCSSQNLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALAVTDITRTEWCEKQTELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSNEDRWALKLLNFIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQMLEINTSRIPMLIDTKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQFFDFFSLNPHSTLSEEIADSTTSFGFTAKTLDDVIRYYMNCCSMLPPTHNQLLLRYESQKDQSIIVEDEFAYDHGWLNSQLETSLQVWCGQREPECTPQEERWKCRHCQFASDCPANANLNATRKPE
Homology
BLAST of HG10001394 vs. NCBI nr
Match: XP_038902050.1 (LOW QUALITY PROTEIN: exonuclease V, chloroplastic [Benincasa hispida])

HSP 1 Score: 704.9 bits (1818), Expect = 4.0e-199
Identity = 363/409 (88.75%), Postives = 377/409 (92.18%), Query Frame = 0

Query: 1   MADSSSGLPPFNNDDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPSC 60
           MADSSSGLPP N+DDNT+E FP IPVEIVSDEEMALIEAALAAAV+TARSSTSAI S  C
Sbjct: 21  MADSSSGLPPINDDDNTNESFPNIPVEIVSDEEMALIEAALAAAVTTARSSTSAIRSTFC 80

Query: 61  SSQNLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALAV 120
           SSQNLFNTRSIHSITLLSKRGI SSQPDIEDLGKIAITQKKIKVNES LQR+ RNK LAV
Sbjct: 81  SSQNLFNTRSIHSITLLSKRGISSSQPDIEDLGKIAITQKKIKVNESFLQRYRRNKPLAV 140

Query: 121 TDITRTEWCEKQTELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSNEDRWALK 180
           TDIT+ EWCEKQ ELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKS EDRWALK
Sbjct: 141 TDITQMEWCEKQMELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSEEDRWALK 200

Query: 181 LLNFIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQMLEINTSRIPMLI 240
           LLNFIFGV QLVLEGLTRELPV        + LV+GVWIVGVIDEIQMLEINTS+IPMLI
Sbjct: 201 LLNFIFGVNQLVLEGLTRELPV--------LSLVEGVWIVGVIDEIQMLEINTSKIPMLI 260

Query: 241 DTKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQFFDFFSLNPHSTLSEEIA 300
           D KTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLIS  FSIRQFFDFFSLNPHSTLSEEIA
Sbjct: 261 DRKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISGSFSIRQFFDFFSLNPHSTLSEEIA 320

Query: 301 DSTTSFGFTAKTLDDVIRYYMNCCSMLPPTHNQLLLRYESQKDQSIIVEDEFAYDHGWLN 360
           DSTTSFGFTAKTLDDV+RYY+NCCSMLPP+HNQLLLRYESQKDQSIIVEDEFAY+HGWL 
Sbjct: 321 DSTTSFGFTAKTLDDVVRYYINCCSMLPPSHNQLLLRYESQKDQSIIVEDEFAYNHGWLK 380

Query: 361 SQLETSLQVWCGQREPECTPQEERWKCRHCQFASDCPANANLNATRKPE 410
           SQLET LQVW GQREPECTP+EERWKCRHCQF SDCP NAN +ATRKPE
Sbjct: 381 SQLETLLQVWGGQREPECTPREERWKCRHCQFVSDCPVNANFDATRKPE 421

BLAST of HG10001394 vs. NCBI nr
Match: XP_022985622.1 (exonuclease V, chloroplastic [Cucurbita maxima] >XP_022985623.1 exonuclease V, chloroplastic [Cucurbita maxima] >XP_022985624.1 exonuclease V, chloroplastic [Cucurbita maxima])

HSP 1 Score: 684.9 bits (1766), Expect = 4.3e-193
Identity = 350/409 (85.57%), Postives = 371/409 (90.71%), Query Frame = 0

Query: 1   MADSSSGLPPFNNDDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPSC 60
           MADSSSGLPP+N+DDNTH+G P IPVEIVSDEEMALIEAALAAAV+TARSS+SAIH PSC
Sbjct: 22  MADSSSGLPPYNDDDNTHKGVPNIPVEIVSDEEMALIEAALAAAVTTARSSSSAIHPPSC 81

Query: 61  SSQNLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALAV 120
           SSQNLFNTRSIHSITLLSKRG+ SSQ D+EDL K + TQKKIKVNESLLQRF RNK LAV
Sbjct: 82  SSQNLFNTRSIHSITLLSKRGLPSSQLDVEDLCKSSSTQKKIKVNESLLQRFRRNKGLAV 141

Query: 121 TDITRTEWCEKQTELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSNEDRWALK 180
           TDITRTEWCEKQ EL LLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKS ED WALK
Sbjct: 142 TDITRTEWCEKQMELCLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSAEDNWALK 201

Query: 181 LLNFIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQMLEINTSRIPMLI 240
           LLNF FGV QL+ EGLTRELPV        MGLV+GVW+VGVIDEIQMLEI T ++PMLI
Sbjct: 202 LLNFTFGVNQLLFEGLTRELPV--------MGLVEGVWMVGVIDEIQMLEIGTCKVPMLI 261

Query: 241 DTKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQFFDFFSLNPHSTLSEEIA 300
           DTKTR+R TIPAEPQ+RNGKLQLMCYKFLLD LI DGFS RQFFDFFSLNPHS LSEEIA
Sbjct: 262 DTKTRMRHTIPAEPQQRNGKLQLMCYKFLLDCLICDGFSTRQFFDFFSLNPHSILSEEIA 321

Query: 301 DSTTSFGFTAKTLDDVIRYYMNCCSMLPPTHNQLLLRYESQKDQSIIVEDEFAYDHGWLN 360
           DS+ SFGFTAKTLDDV+RYY+NCCSMLPPTHNQLLLRYESQKDQSII EDEFAYDHGWL 
Sbjct: 322 DSSASFGFTAKTLDDVVRYYINCCSMLPPTHNQLLLRYESQKDQSIIREDEFAYDHGWLK 381

Query: 361 SQLETSLQVWCGQREPECTPQEERWKCRHCQFASDCPANANLNATRKPE 410
           SQLETSLQVW GQREPECTP EERWKCRHCQ+ASDCPANA+L+ TRKPE
Sbjct: 382 SQLETSLQVWHGQREPECTPPEERWKCRHCQYASDCPANASLDPTRKPE 422

BLAST of HG10001394 vs. NCBI nr
Match: XP_023511975.1 (LOW QUALITY PROTEIN: exonuclease V, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 684.5 bits (1765), Expect = 5.7e-193
Identity = 352/409 (86.06%), Postives = 370/409 (90.46%), Query Frame = 0

Query: 1   MADSSSGLPPFNNDDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPSC 60
           MADSSSGLPPFN+DDNTH+G P IPVEIVSDEEMALIEAALAAAV+TARSS+SAIH PSC
Sbjct: 22  MADSSSGLPPFNDDDNTHKGVPNIPVEIVSDEEMALIEAALAAAVTTARSSSSAIHPPSC 81

Query: 61  SSQNLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALAV 120
           SSQNLFNTRSIHSITLLSKRG+ SSQ DIEDL K + TQKKIKVNESLLQRF RNK LAV
Sbjct: 82  SSQNLFNTRSIHSITLLSKRGLPSSQLDIEDLCKSSSTQKKIKVNESLLQRFRRNKGLAV 141

Query: 121 TDITRTEWCEKQTELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSNEDRWALK 180
           TDITRTEWCEKQ EL LLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKS ED WALK
Sbjct: 142 TDITRTEWCEKQMELCLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSAEDNWALK 201

Query: 181 LLNFIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQMLEINTSRIPMLI 240
           LLNF FGV QL+ EGLTRELPV        MGLV+GVW+VGVIDEIQMLEI T ++PMLI
Sbjct: 202 LLNFTFGVNQLLFEGLTRELPV--------MGLVEGVWMVGVIDEIQMLEIGTCKVPMLI 261

Query: 241 DTKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQFFDFFSLNPHSTLSEEIA 300
           DTKTR+R TIPAEPQ+RNGKLQLMCYKFLLD LI DGFS RQFFDFFSLNPHS LSEEIA
Sbjct: 262 DTKTRMRHTIPAEPQQRNGKLQLMCYKFLLDCLICDGFSTRQFFDFFSLNPHSILSEEIA 321

Query: 301 DSTTSFGFTAKTLDDVIRYYMNCCSMLPPTHNQLLLRYESQKDQSIIVEDEFAYDHGWLN 360
           DST S GFTAKTLDDV+RYY+NCCSMLPPTHNQLLLRYESQKDQSII EDEFAYDHGWL 
Sbjct: 322 DSTASSGFTAKTLDDVVRYYINCCSMLPPTHNQLLLRYESQKDQSIIGEDEFAYDHGWLK 381

Query: 361 SQLETSLQVWCGQREPECTPQEERWKCRHCQFASDCPANANLNATRKPE 410
           SQLETSLQVW GQREPECTP EERWKCRHCQ+ASDCPANA+L+ TRKPE
Sbjct: 382 SQLETSLQVWHGQREPECTPLEERWKCRHCQYASDCPANASLDPTRKPE 422

BLAST of HG10001394 vs. NCBI nr
Match: KAG6570766.1 (Exonuclease V, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 681.4 bits (1757), Expect = 4.8e-192
Identity = 350/409 (85.57%), Postives = 368/409 (89.98%), Query Frame = 0

Query: 1   MADSSSGLPPFNNDDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPSC 60
           MADSSSGLPPFN+DDNTH+  P IPVEIVSDEEMALIEAALAAAV+TARSS+SAIH PSC
Sbjct: 1   MADSSSGLPPFNDDDNTHKDIPNIPVEIVSDEEMALIEAALAAAVNTARSSSSAIHPPSC 60

Query: 61  SSQNLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALAV 120
           SSQNLF+TRSIHSITLLSKRG+ SSQ DIEDL K + TQKKIKVNESLLQRF RNK LAV
Sbjct: 61  SSQNLFHTRSIHSITLLSKRGLPSSQLDIEDLCKSSSTQKKIKVNESLLQRFRRNKGLAV 120

Query: 121 TDITRTEWCEKQTELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSNEDRWALK 180
           TDITRTEWCEKQ EL LLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKS ED WALK
Sbjct: 121 TDITRTEWCEKQMELCLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSAEDNWALK 180

Query: 181 LLNFIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQMLEINTSRIPMLI 240
           LLNF FGV QL+ EGLTRELPV        MGLV+GVW+VGVIDEIQMLEI T ++PMLI
Sbjct: 181 LLNFTFGVNQLLFEGLTRELPV--------MGLVEGVWMVGVIDEIQMLEIGTCKVPMLI 240

Query: 241 DTKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQFFDFFSLNPHSTLSEEIA 300
           DTKTR+R TIPAEPQ+RNGKLQLMCYKFLLD LI DGFS RQFFDFFSLNPHS LSEEIA
Sbjct: 241 DTKTRMRHTIPAEPQQRNGKLQLMCYKFLLDCLICDGFSTRQFFDFFSLNPHSILSEEIA 300

Query: 301 DSTTSFGFTAKTLDDVIRYYMNCCSMLPPTHNQLLLRYESQKDQSIIVEDEFAYDHGWLN 360
           DST SFGFTAKTLDDV+RYY+NCCSMLPP HNQLLLRYESQKDQSII EDEFAYDHGWL 
Sbjct: 301 DSTVSFGFTAKTLDDVVRYYINCCSMLPPVHNQLLLRYESQKDQSIIGEDEFAYDHGWLK 360

Query: 361 SQLETSLQVWCGQREPECTPQEERWKCRHCQFASDCPANANLNATRKPE 410
           SQLETSLQVW GQREPECTP EERWKCRHCQ+ASDCPANA+L  TRKPE
Sbjct: 361 SQLETSLQVWHGQREPECTPPEERWKCRHCQYASDCPANASLKPTRKPE 401

BLAST of HG10001394 vs. NCBI nr
Match: XP_022943527.1 (LOW QUALITY PROTEIN: exonuclease V, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 679.9 bits (1753), Expect = 1.4e-191
Identity = 349/409 (85.33%), Postives = 369/409 (90.22%), Query Frame = 0

Query: 1   MADSSSGLPPFNNDDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPSC 60
           MADSSSGLPPFN+DDNTH+  P IPVEIVSDEEMALIEAALAAAV+TARSS+SAIH PSC
Sbjct: 22  MADSSSGLPPFNDDDNTHKDVPNIPVEIVSDEEMALIEAALAAAVTTARSSSSAIHPPSC 81

Query: 61  SSQNLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALAV 120
           SSQNLFNTRSIHSITLLSKRG+ SSQ DIEDL K + TQKKIKVNESLLQRF R K LAV
Sbjct: 82  SSQNLFNTRSIHSITLLSKRGLPSSQLDIEDLCKSSSTQKKIKVNESLLQRFRRTKGLAV 141

Query: 121 TDITRTEWCEKQTELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSNEDRWALK 180
           TDITRTEWCEKQ EL LLSGVRKKTKAMEAGIARHAMLE+EVVKKVKVQVKS ED WALK
Sbjct: 142 TDITRTEWCEKQMELCLLSGVRKKTKAMEAGIARHAMLESEVVKKVKVQVKSAEDNWALK 201

Query: 181 LLNFIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQMLEINTSRIPMLI 240
           LLNF FGV QL+ EGLTRELPV        MGLV+GVW+VGVIDEIQMLEI T ++PMLI
Sbjct: 202 LLNFTFGVNQLLFEGLTRELPV--------MGLVEGVWMVGVIDEIQMLEIGTCKVPMLI 261

Query: 241 DTKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQFFDFFSLNPHSTLSEEIA 300
           DTKTR+R TIPAEPQ+RNGKLQLMCYKFLLD LI DGFS RQFFDFFSLNPHS LSEEIA
Sbjct: 262 DTKTRMRHTIPAEPQQRNGKLQLMCYKFLLDCLICDGFSTRQFFDFFSLNPHSILSEEIA 321

Query: 301 DSTTSFGFTAKTLDDVIRYYMNCCSMLPPTHNQLLLRYESQKDQSIIVEDEFAYDHGWLN 360
           DST SFGFTAKTLDDV+RYY+NCCSMLPPTHNQLLLRYESQKDQSII EDEFAYDHGWL 
Sbjct: 322 DSTVSFGFTAKTLDDVVRYYINCCSMLPPTHNQLLLRYESQKDQSIIGEDEFAYDHGWLK 381

Query: 361 SQLETSLQVWCGQREPECTPQEERWKCRHCQFASDCPANANLNATRKPE 410
           SQLETSLQVW GQREPECTP EERWKCRHCQ+AS+CPANA+L+ TRKPE
Sbjct: 382 SQLETSLQVWHGQREPECTPPEERWKCRHCQYASNCPANASLDPTRKPE 422

BLAST of HG10001394 vs. ExPASy Swiss-Prot
Match: Q9FKK6 (Exonuclease V, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g60370 PE=2 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.0e-96
Identity = 201/403 (49.88%), Postives = 266/403 (66.00%), Query Frame = 0

Query: 4   SSSGLPPFNNDDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPSCSSQ 63
           + SG    ++  + H  FP+IP+EIVS+EEMA+++AALAA+ S   S   ++ SPS  + 
Sbjct: 22  AQSGTVSISSPQSHHVVFPEIPIEIVSEEEMAILDAALAASRSILPSVIRSV-SPSRITA 81

Query: 64  NLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALAVTDI 123
              + ++I SITL SKR + S+  DIE               ES L RF RN+AL VTD+
Sbjct: 82  G-GSPKTIRSITLFSKRKL-SACSDIE---------------ESYLHRFRRNQALGVTDL 141

Query: 124 TRTEWCEKQTELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSNEDRWALKLLN 183
           T TEWCEKQ E  L  G RK  KAM+ G ARH  LE EVVKKV+V+V+SNED+WALKLLN
Sbjct: 142 TGTEWCEKQMENVLCFGRRKVNKAMKVGQARHLQLEEEVVKKVRVKVESNEDKWALKLLN 201

Query: 184 FIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQ--MLEINTSRIPMLID 243
            I GV Q + EG TREL        + +G V G WIVG+IDE++    E ++   P+LID
Sbjct: 202 SIAGVNQFLFEGRTREL--------LLLGFVGGQWIVGIIDELRKAYAEESSDSGPILID 261

Query: 244 TKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQFFDFFSLNPHSTLSEEIAD 303
           TKTR+RDT+PAEPQRRNG+LQLM YK L D+++ +GF    FFD+FSLN +  LS+++ D
Sbjct: 262 TKTRLRDTLPAEPQRRNGRLQLMLYKLLWDTIVKEGFPTGPFFDYFSLNRNYVLSQDVRD 321

Query: 304 STTSFGFTAKTLDDVIRYYMNCCSMLPPTHNQLLLRYESQKDQSIIVEDEFAYDHGWLNS 363
              + G  A+TL++++RYY N   MLP  +NQLLL+YE QKDQSII E  F +DH W+ S
Sbjct: 322 HIANAGIKAQTLEEIVRYYENTFQMLPIANNQLLLKYEFQKDQSIIAEIRFNHDHEWVMS 381

Query: 364 QLETSLQVWCGQREPECTPQEERWKCRHCQFASDCPANANLNA 405
           +    ++ W  +RE   TP+EERWKCR+CQFA  CP N +  +
Sbjct: 382 KYREVIEFWRNEREAGYTPEEERWKCRYCQFAKSCPGNPSFES 398

BLAST of HG10001394 vs. ExPASy Swiss-Prot
Match: A2VDX7 (Exonuclease V OS=Bos taurus OX=9913 GN=EXO5 PE=2 SV=2)

HSP 1 Score: 125.2 bits (313), Expect = 1.7e-27
Identity = 88/310 (28.39%), Postives = 146/310 (47.10%), Query Frame = 0

Query: 107 SLLQRFMRNKALAVTDITRTEWCEKQTELSL-LSG--VRKKTKAMEAGIARHAMLEAEVV 166
           S ++RF   K L VTD++   WCE+Q       SG    +K+  ++ G + H   E EV 
Sbjct: 68  SPMERF-HLKYLYVTDLSTQNWCEQQMVYGKEFSGFLTPEKSAILDTGASIHLARELEVH 127

Query: 167 KKVKVQVKSNEDRWALKLLNFIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVI 226
             V + + S ED WA+K LN +  +  L  EG  RE PV           V+GV +VGVI
Sbjct: 128 DLVSIPITSKEDAWAVKFLNILSMIPTLQSEGRIREFPV--------FAEVEGVLLVGVI 187

Query: 227 DEIQMLEINTSRIPMLIDTKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQF 286
           DE   L    S    L + KTR    +P++ Q++    Q+  YK++ D+++    +    
Sbjct: 188 DE---LHYTASGELELTELKTRGNPVLPSDAQKKKDYFQVSLYKYIFDAMVQGKVTAASL 247

Query: 287 FDFFSLNPHSTLSEEIADSTTSFGFTAKTLDDVIR-YYMNCCSMLPPTHNQLLLRYESQK 346
                L+P   L   +       G++ K+L D+I   +++      P  + L + Y  Q 
Sbjct: 248 IHHTKLDPEKPLGPSVLRHAQQGGYSVKSLGDLIELVFLSLTLSDLPLIDSLKIEYVHQG 307

Query: 347 DQSIIVEDEFAYDHGWLNSQLETSLQVWCGQREPECTPQEERWKCRHCQFASDC---PAN 406
             +++  +  A+    + S+++  +  W G REP+    EE WKCR C +A  C    + 
Sbjct: 308 TATVLGTEMVAFAEKEVRSKVQHYMTYWMGHREPQGVDVEEAWKCRMCNYADICEWKKSG 365

Query: 407 ANLNATRKPE 410
             ++AT +P+
Sbjct: 368 GLISATLEPQ 365

BLAST of HG10001394 vs. ExPASy Swiss-Prot
Match: Q9H790 (Exonuclease V OS=Homo sapiens OX=9606 GN=EXO5 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.4e-24
Identity = 94/328 (28.66%), Postives = 148/328 (45.12%), Query Frame = 0

Query: 78  SKRGIYSSQPDIEDLGK----IAITQKKIKVN-ESLLQRFMRNKALAVTDITRTEWCEKQ 137
           SK  +    P  E LGK    I++   K  ++  S ++RF   K L VTD+    WCE Q
Sbjct: 37  SKALVNMPGPSSESLGKDDKPISLQNWKRGLDILSPMERF-HLKYLYVTDLATQNWCELQ 96

Query: 138 TELSL-LSG--VRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSNEDRWALKLLNFIFGVK 197
           T     L G    +K   ++ G + H   E E+   V V V + ED WA+K LN +  + 
Sbjct: 97  TAYGKELPGFLAPEKAAVLDTGASIHLARELELHDLVTVPVTTKEDAWAIKFLNILLLIP 156

Query: 198 QLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQMLEINTSRIPMLIDTKTRVRDT 257
            L  EG  RE PV         G  +GV +VGVIDE   L         L + KTR R  
Sbjct: 157 TLQSEGHIREFPV--------FGEGEGVLLVGVIDE---LHYTAKGELELAELKTRRRPM 216

Query: 258 IPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQFFDFFSLNPHSTLSEEIADSTTSFGFT 317
           +P E Q++    Q+  YK++ D+++    +         L     L   +       GF+
Sbjct: 217 LPLEAQKKKDCFQVSLYKYIFDAMVQGKVTPASLIHHTKLCLEKPLGPSVLRHAQQGGFS 276

Query: 318 AKTLDDVIR-YYMNCCSMLPPTHNQLLLRYESQKDQSIIVEDEFAYDHGWLNSQLETSLQ 377
            K+L D++   +++      P  + L + Y  Q+  +++  +  A+    + ++++  + 
Sbjct: 277 VKSLGDLMELVFLSLTLSDLPVIDILKIEYIHQETATVLGTEIVAFKEKEVRAKVQHYMA 336

Query: 378 VWCGQREPECTPQEERWKCRHCQFASDC 397
            W G REP+    EE WKCR C +A  C
Sbjct: 337 YWMGHREPQGVDVEEAWKCRTCTYADIC 352

BLAST of HG10001394 vs. ExPASy Swiss-Prot
Match: Q9CXP9 (Exonuclease V OS=Mus musculus OX=10090 GN=Exo5 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 8.9e-24
Identity = 83/295 (28.14%), Postives = 137/295 (46.44%), Query Frame = 0

Query: 107 SLLQRFMRNKALAVTDITRTEWCEKQTELSL-LSG--VRKKTKAMEAGIARHAMLEAEVV 166
           S ++RF   K L VTD+    WCE Q      L G    +K   ++ G + H   E E+ 
Sbjct: 71  SPMERF-HLKYLYVTDLCTQNWCELQMVYGKELPGSLTPEKAAVLDTGASIHLAKELELH 130

Query: 167 KKVKVQVKSNEDRWALKLLNFIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVI 226
             V V + + ED WA+K LN +  +  L  EG  RE PV         G V+G+++VGVI
Sbjct: 131 DLVTVPIATKEDAWAVKFLNILAMIPALQSEGRVREFPV--------FGEVEGIFLVGVI 190

Query: 227 DEIQMLEINTSRIPM-LIDTKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQ 286
           DE+      TS+  + L + KTR R  +P   Q++    Q+  YK++ D+++    +   
Sbjct: 191 DELHY----TSKGELELAELKTRRRPVLPLPAQKKKDYFQVSLYKYIFDAMVQGKVTPAS 250

Query: 287 FFDFFSLNPHSTLSEEIADSTTSFGFTAKTLDDVIR-YYMNCCSMLPPTHNQLLLRYESQ 346
                 L     L   +       G + K+L D++   +++      P  + L L Y  Q
Sbjct: 251 LIHHTKLCLDKPLGPSVLRHARQGGVSVKSLGDLMELVFLSLTLSDLPAIDTLKLEYIHQ 310

Query: 347 KDQSIIVEDEFAYDHGWLNSQLETSLQVWCGQREPECTPQEERWKCRHCQFASDC 397
           +  +I+  +  A++   + S+++  +  W G R+P+    EE WKCR C +   C
Sbjct: 311 ETATILGTEIVAFEEKEVKSKVQHYVAYWMGHRDPQGVDVEEAWKCRTCDYVDIC 352

BLAST of HG10001394 vs. ExPASy Swiss-Prot
Match: F1Q514 (Exonuclease V OS=Danio rerio OX=7955 GN=exo5 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 3.4e-23
Identity = 115/408 (28.19%), Postives = 176/408 (43.14%), Query Frame = 0

Query: 1   MADSSSGLPPFNN-DDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPS 60
           M D+S    P +  DD +   F  IP    SDEE   IE+ L     T    +  +  PS
Sbjct: 1   MMDASRSQQPLDAWDDISDSEFLNIP----SDEES--IESQLPLIGETKGKHSVNLDKPS 60

Query: 61  CSSQNLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALA 120
            SS       S H  +  + +       D+  L + ++ +       S +QRF R + L+
Sbjct: 61  SSS-------SFHKCSEEAPKQEAVKTDDVRGLKRKSLHENSF----SPMQRF-RKQHLS 120

Query: 121 VTDITRTEWCEKQTELSLLSG--VRKKTKAMEAGIAR--HAMLEAEVVKKVKVQVKSNED 180
           VT +    WCE ++  +LL     RK+ +  E  I +  H   E E+   V V +++ ED
Sbjct: 121 VTLLCDQTWCEMKSVYNLLKPHIKRKEMQRTEVQIGQEIHLSRELEIQDVVPVDIRTRED 180

Query: 181 RWALKLLNFIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQMLEINTSR 240
             A+KLLN +  +  L      RE PV         G+ +GV+I+GVIDE   L  N   
Sbjct: 181 GEAVKLLNMLHMIPLLEAGQRVREFPV--------FGVQEGVFIMGVIDE---LMYNQKG 240

Query: 241 IPMLIDTKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQFFDFFSLNPHSTL 300
             +L + KTR ++++P+  Q +    Q+  YK L D L+         FD   L     L
Sbjct: 241 ELVLNELKTRRQNSLPSSAQDKVNCFQVGLYKLLFDGLVRGEMKKNHIFDHLKLRSAQIL 300

Query: 301 SEEIADSTTSFGFTAKTLDDVIRYYM--NCCSMLPPTHNQLLLRYESQKDQSIIVEDEFA 360
              +     + G  A+T ++++   +    CS L P  + L + Y  Q     I      
Sbjct: 301 GAGVQAHAKNLGVHARTFEELVETLLITVSCSDL-PCIDLLQIEYFHQGSNGPIGTRVAP 360

Query: 361 YDHGWLNSQLETSLQVWCGQREPECTPQEERWKCRHCQFASDCPANAN 402
           +D   +  +L+  L  W GQREP+    EE WKCR C +   C    N
Sbjct: 361 FDEAQIRGELQAYLSYWTGQREPKGVDIEEAWKCRSCLYEEICEWRKN 378

BLAST of HG10001394 vs. ExPASy TrEMBL
Match: A0A6J1J5D7 (exonuclease V, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483632 PE=3 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 2.1e-193
Identity = 350/409 (85.57%), Postives = 371/409 (90.71%), Query Frame = 0

Query: 1   MADSSSGLPPFNNDDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPSC 60
           MADSSSGLPP+N+DDNTH+G P IPVEIVSDEEMALIEAALAAAV+TARSS+SAIH PSC
Sbjct: 22  MADSSSGLPPYNDDDNTHKGVPNIPVEIVSDEEMALIEAALAAAVTTARSSSSAIHPPSC 81

Query: 61  SSQNLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALAV 120
           SSQNLFNTRSIHSITLLSKRG+ SSQ D+EDL K + TQKKIKVNESLLQRF RNK LAV
Sbjct: 82  SSQNLFNTRSIHSITLLSKRGLPSSQLDVEDLCKSSSTQKKIKVNESLLQRFRRNKGLAV 141

Query: 121 TDITRTEWCEKQTELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSNEDRWALK 180
           TDITRTEWCEKQ EL LLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKS ED WALK
Sbjct: 142 TDITRTEWCEKQMELCLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSAEDNWALK 201

Query: 181 LLNFIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQMLEINTSRIPMLI 240
           LLNF FGV QL+ EGLTRELPV        MGLV+GVW+VGVIDEIQMLEI T ++PMLI
Sbjct: 202 LLNFTFGVNQLLFEGLTRELPV--------MGLVEGVWMVGVIDEIQMLEIGTCKVPMLI 261

Query: 241 DTKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQFFDFFSLNPHSTLSEEIA 300
           DTKTR+R TIPAEPQ+RNGKLQLMCYKFLLD LI DGFS RQFFDFFSLNPHS LSEEIA
Sbjct: 262 DTKTRMRHTIPAEPQQRNGKLQLMCYKFLLDCLICDGFSTRQFFDFFSLNPHSILSEEIA 321

Query: 301 DSTTSFGFTAKTLDDVIRYYMNCCSMLPPTHNQLLLRYESQKDQSIIVEDEFAYDHGWLN 360
           DS+ SFGFTAKTLDDV+RYY+NCCSMLPPTHNQLLLRYESQKDQSII EDEFAYDHGWL 
Sbjct: 322 DSSASFGFTAKTLDDVVRYYINCCSMLPPTHNQLLLRYESQKDQSIIREDEFAYDHGWLK 381

Query: 361 SQLETSLQVWCGQREPECTPQEERWKCRHCQFASDCPANANLNATRKPE 410
           SQLETSLQVW GQREPECTP EERWKCRHCQ+ASDCPANA+L+ TRKPE
Sbjct: 382 SQLETSLQVWHGQREPECTPPEERWKCRHCQYASDCPANASLDPTRKPE 422

BLAST of HG10001394 vs. ExPASy TrEMBL
Match: A0A6J1FT99 (LOW QUALITY PROTEIN: exonuclease V, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111448276 PE=3 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 6.7e-192
Identity = 349/409 (85.33%), Postives = 369/409 (90.22%), Query Frame = 0

Query: 1   MADSSSGLPPFNNDDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPSC 60
           MADSSSGLPPFN+DDNTH+  P IPVEIVSDEEMALIEAALAAAV+TARSS+SAIH PSC
Sbjct: 22  MADSSSGLPPFNDDDNTHKDVPNIPVEIVSDEEMALIEAALAAAVTTARSSSSAIHPPSC 81

Query: 61  SSQNLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALAV 120
           SSQNLFNTRSIHSITLLSKRG+ SSQ DIEDL K + TQKKIKVNESLLQRF R K LAV
Sbjct: 82  SSQNLFNTRSIHSITLLSKRGLPSSQLDIEDLCKSSSTQKKIKVNESLLQRFRRTKGLAV 141

Query: 121 TDITRTEWCEKQTELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSNEDRWALK 180
           TDITRTEWCEKQ EL LLSGVRKKTKAMEAGIARHAMLE+EVVKKVKVQVKS ED WALK
Sbjct: 142 TDITRTEWCEKQMELCLLSGVRKKTKAMEAGIARHAMLESEVVKKVKVQVKSAEDNWALK 201

Query: 181 LLNFIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQMLEINTSRIPMLI 240
           LLNF FGV QL+ EGLTRELPV        MGLV+GVW+VGVIDEIQMLEI T ++PMLI
Sbjct: 202 LLNFTFGVNQLLFEGLTRELPV--------MGLVEGVWMVGVIDEIQMLEIGTCKVPMLI 261

Query: 241 DTKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQFFDFFSLNPHSTLSEEIA 300
           DTKTR+R TIPAEPQ+RNGKLQLMCYKFLLD LI DGFS RQFFDFFSLNPHS LSEEIA
Sbjct: 262 DTKTRMRHTIPAEPQQRNGKLQLMCYKFLLDCLICDGFSTRQFFDFFSLNPHSILSEEIA 321

Query: 301 DSTTSFGFTAKTLDDVIRYYMNCCSMLPPTHNQLLLRYESQKDQSIIVEDEFAYDHGWLN 360
           DST SFGFTAKTLDDV+RYY+NCCSMLPPTHNQLLLRYESQKDQSII EDEFAYDHGWL 
Sbjct: 322 DSTVSFGFTAKTLDDVVRYYINCCSMLPPTHNQLLLRYESQKDQSIIGEDEFAYDHGWLK 381

Query: 361 SQLETSLQVWCGQREPECTPQEERWKCRHCQFASDCPANANLNATRKPE 410
           SQLETSLQVW GQREPECTP EERWKCRHCQ+AS+CPANA+L+ TRKPE
Sbjct: 382 SQLETSLQVWHGQREPECTPPEERWKCRHCQYASNCPANASLDPTRKPE 422

BLAST of HG10001394 vs. ExPASy TrEMBL
Match: A0A5A7SXN1 (Exonuclease V OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00520 PE=3 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 8.8e-192
Identity = 353/401 (88.03%), Postives = 367/401 (91.52%), Query Frame = 0

Query: 1   MADSSSGLPPFNNDDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPSC 60
           MADS SGLPPF +DDNTHEGFP IPVEIVSDEEMALIEAALAAAV+T+RSS+S IHS SC
Sbjct: 1   MADSHSGLPPFKDDDNTHEGFPNIPVEIVSDEEMALIEAALAAAVTTSRSSSSVIHSTSC 60

Query: 61  SSQNLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALAV 120
           SS NLFNTRSIHSITLLSKRGI SSQPDIEDLGKI ITQKKIKVNESLLQRF RNK LAV
Sbjct: 61  SSHNLFNTRSIHSITLLSKRGIDSSQPDIEDLGKIPITQKKIKVNESLLQRFKRNKPLAV 120

Query: 121 TDITRTEWCEKQTELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSNEDRWALK 180
           TDITR EWCEKQ E SLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQV+S ED WALK
Sbjct: 121 TDITRMEWCEKQMEFSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVQSFEDIWALK 180

Query: 181 LLNFIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQMLEINTSRIPMLI 240
           LLNFI GV QLVLEGLTRELPV        MGLV+GVWIVG+IDEIQMLEINT++IPMLI
Sbjct: 181 LLNFIVGVHQLVLEGLTRELPV--------MGLVEGVWIVGIIDEIQMLEINTTKIPMLI 240

Query: 241 DTKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLI-SDGFSIRQFFDFFSLNPHSTLSEEI 300
           DTKTRVRDTIPA PQRRNGKLQLM YK LLD+LI SDGF IRQFFDFFSLNPHSTLSEEI
Sbjct: 241 DTKTRVRDTIPAVPQRRNGKLQLMFYKLLLDNLISSDGFPIRQFFDFFSLNPHSTLSEEI 300

Query: 301 ADSTTSFGFTAKTLDDVIRYYMNCCSMLPPTHNQLLLRYESQKDQSIIVEDEFAYDHGWL 360
           ADS+TSFGFTAKTLDDV+RYY+NCCSMLPPTHNQLLLRYESQKDQSIIVED+FAYDHGWL
Sbjct: 301 ADSSTSFGFTAKTLDDVVRYYINCCSMLPPTHNQLLLRYESQKDQSIIVEDKFAYDHGWL 360

Query: 361 NSQLETSLQVWCGQREPECTPQEERWKCRHCQFASDCPANA 401
            SQLE  LQVW GQREPECTPQEERWKCRHCQFASDCPANA
Sbjct: 361 KSQLEIQLQVWHGQREPECTPQEERWKCRHCQFASDCPANA 393

BLAST of HG10001394 vs. ExPASy TrEMBL
Match: A0A0A0KC56 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G290860 PE=3 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 1.1e-189
Identity = 345/400 (86.25%), Postives = 366/400 (91.50%), Query Frame = 0

Query: 1   MADSSSGLPPFNNDDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPSC 60
           MADS SGLPPF +DDNTHEGFP IPVEIVSDEEMALIEAALAAAV+T+RSS+S IHS S 
Sbjct: 1   MADSHSGLPPFKDDDNTHEGFPNIPVEIVSDEEMALIEAALAAAVTTSRSSSSVIHSTSS 60

Query: 61  SSQNLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALAV 120
           SS +LFNTRSIHSITLLSKRGI+S+QPDIEDLGKI ITQKKIKVNESLLQRF RNK LAV
Sbjct: 61  SSHSLFNTRSIHSITLLSKRGIHSTQPDIEDLGKIPITQKKIKVNESLLQRFKRNKPLAV 120

Query: 121 TDITRTEWCEKQTELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSNEDRWALK 180
           TDITR EWCEKQ E SLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQV+S ED WALK
Sbjct: 121 TDITRMEWCEKQMEFSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVQSIEDIWALK 180

Query: 181 LLNFIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQMLEINTSRIPMLI 240
           LLNFI GV QLVLEGLTRELPV        MGLV+GVWIVG+IDEIQMLEI+T++IPMLI
Sbjct: 181 LLNFIVGVHQLVLEGLTRELPV--------MGLVEGVWIVGIIDEIQMLEIDTTKIPMLI 240

Query: 241 DTKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQFFDFFSLNPHSTLSEEIA 300
           DTKTRVRDTIPA PQRRNGKLQLM YKFLLD+LISDG SIRQFFDFFSLNPHSTLSEEI+
Sbjct: 241 DTKTRVRDTIPAVPQRRNGKLQLMFYKFLLDNLISDGLSIRQFFDFFSLNPHSTLSEEIS 300

Query: 301 DSTTSFGFTAKTLDDVIRYYMNCCSMLPPTHNQLLLRYESQKDQSIIVEDEFAYDHGWLN 360
           D+TTS GFTAKTLDDV+RYY+NCCSMLPPTHNQLL+RYESQKDQSIIVED+FAYDHGWL 
Sbjct: 301 DTTTSIGFTAKTLDDVVRYYINCCSMLPPTHNQLLIRYESQKDQSIIVEDKFAYDHGWLK 360

Query: 361 SQLETSLQVWCGQREPECTPQEERWKCRHCQFASDCPANA 401
           +QLET LQVW GQREPECTPQEERWKCRHCQFA DCP NA
Sbjct: 361 TQLETQLQVWHGQREPECTPQEERWKCRHCQFAFDCPTNA 392

BLAST of HG10001394 vs. ExPASy TrEMBL
Match: A0A1S3CLU8 (LOW QUALITY PROTEIN: exonuclease V, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501929 PE=3 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 1.1e-189
Identity = 350/401 (87.28%), Postives = 364/401 (90.77%), Query Frame = 0

Query: 1   MADSSSGLPPFNNDDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPSC 60
           MADS SGLPPF +DDNTHEGFP IPVEIVSDEEMALIEAALAAAV+T+RSS+S IHS SC
Sbjct: 1   MADSHSGLPPFKDDDNTHEGFPNIPVEIVSDEEMALIEAALAAAVTTSRSSSSVIHSTSC 60

Query: 61  SSQNLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALAV 120
           SS NLFNTRSIHSITLLSKRGI SSQPDIEDLGKI ITQKKIKVNESLLQRF RNK LAV
Sbjct: 61  SSHNLFNTRSIHSITLLSKRGIDSSQPDIEDLGKIPITQKKIKVNESLLQRFKRNKPLAV 120

Query: 121 TDITRTEWCEKQTELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSNEDRWALK 180
           TDITR EWCEKQ E SLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQV+S ED WALK
Sbjct: 121 TDITRMEWCEKQMEFSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVQSFEDIWALK 180

Query: 181 LLNFIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQMLEINTSRIPMLI 240
           LLNFI GV QLVLEGLTRELPV        MGLV+GVWIVG+IDEIQMLEINT++IPMLI
Sbjct: 181 LLNFIVGVHQLVLEGLTRELPV--------MGLVEGVWIVGIIDEIQMLEINTTKIPMLI 240

Query: 241 DTKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLI-SDGFSIRQFFDFFSLNPHSTLSEEI 300
           DTKTRVRDTIPA PQRRNGKLQLM YK LLD+LI SDGF    FFDFFSLNPHSTLSEEI
Sbjct: 241 DTKTRVRDTIPAVPQRRNGKLQLMFYKLLLDNLISSDGFPSDNFFDFFSLNPHSTLSEEI 300

Query: 301 ADSTTSFGFTAKTLDDVIRYYMNCCSMLPPTHNQLLLRYESQKDQSIIVEDEFAYDHGWL 360
           ADS+TSFGFTAKTLDDV+RYY+NCCSMLPPTHNQLLLRYESQKDQSIIVED+FAYDHGWL
Sbjct: 301 ADSSTSFGFTAKTLDDVVRYYINCCSMLPPTHNQLLLRYESQKDQSIIVEDKFAYDHGWL 360

Query: 361 NSQLETSLQVWCGQREPECTPQEERWKCRHCQFASDCPANA 401
            SQLE  LQVW GQREPECTPQEERWKCRHCQFASDCPANA
Sbjct: 361 KSQLEIQLQVWHGQREPECTPQEERWKCRHCQFASDCPANA 393

BLAST of HG10001394 vs. TAIR 10
Match: AT5G60370.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; CONTAINS InterPro DOMAIN/s: Defects-in-morphology protein 1-like, mitochondrial (InterPro:IPR019190); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G57110.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 353.6 bits (906), Expect = 2.1e-97
Identity = 201/403 (49.88%), Postives = 266/403 (66.00%), Query Frame = 0

Query: 4   SSSGLPPFNNDDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPSCSSQ 63
           + SG    ++  + H  FP+IP+EIVS+EEMA+++AALAA+ S   S   ++ SPS  + 
Sbjct: 22  AQSGTVSISSPQSHHVVFPEIPIEIVSEEEMAILDAALAASRSILPSVIRSV-SPSRITA 81

Query: 64  NLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALAVTDI 123
              + ++I SITL SKR + S+  DIE               ES L RF RN+AL VTD+
Sbjct: 82  G-GSPKTIRSITLFSKRKL-SACSDIE---------------ESYLHRFRRNQALGVTDL 141

Query: 124 TRTEWCEKQTELSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSNEDRWALKLLN 183
           T TEWCEKQ E  L  G RK  KAM+ G ARH  LE EVVKKV+V+V+SNED+WALKLLN
Sbjct: 142 TGTEWCEKQMENVLCFGRRKVNKAMKVGQARHLQLEEEVVKKVRVKVESNEDKWALKLLN 201

Query: 184 FIFGVKQLVLEGLTRELPVSYVCTGVRMGLVDGVWIVGVIDEIQ--MLEINTSRIPMLID 243
            I GV Q + EG TREL        + +G V G WIVG+IDE++    E ++   P+LID
Sbjct: 202 SIAGVNQFLFEGRTREL--------LLLGFVGGQWIVGIIDELRKAYAEESSDSGPILID 261

Query: 244 TKTRVRDTIPAEPQRRNGKLQLMCYKFLLDSLISDGFSIRQFFDFFSLNPHSTLSEEIAD 303
           TKTR+RDT+PAEPQRRNG+LQLM YK L D+++ +GF    FFD+FSLN +  LS+++ D
Sbjct: 262 TKTRLRDTLPAEPQRRNGRLQLMLYKLLWDTIVKEGFPTGPFFDYFSLNRNYVLSQDVRD 321

Query: 304 STTSFGFTAKTLDDVIRYYMNCCSMLPPTHNQLLLRYESQKDQSIIVEDEFAYDHGWLNS 363
              + G  A+TL++++RYY N   MLP  +NQLLL+YE QKDQSII E  F +DH W+ S
Sbjct: 322 HIANAGIKAQTLEEIVRYYENTFQMLPIANNQLLLKYEFQKDQSIIAEIRFNHDHEWVMS 381

Query: 364 QLETSLQVWCGQREPECTPQEERWKCRHCQFASDCPANANLNA 405
           +    ++ W  +RE   TP+EERWKCR+CQFA  CP N +  +
Sbjct: 382 KYREVIEFWRNEREAGYTPEEERWKCRYCQFAKSCPGNPSFES 398

BLAST of HG10001394 vs. TAIR 10
Match: AT3G57110.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G60370.1); Has 16 Blast hits to 16 proteins in 5 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 16; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 51.2 bits (121), Expect = 2.3e-06
Identity = 42/123 (34.15%), Postives = 67/123 (54.47%), Query Frame = 0

Query: 4   SSSGLPPFNNDDNTHEGFPKIPVEIVSDEEMALIEAALAAAVSTARSSTSAIHSPSCSSQ 63
           + SG    ++  + H  FP+IP+EI+++EE+ +++AALAA+ S   S   ++ SPS    
Sbjct: 22  AQSGTVSISSPQSHHVVFPEIPIEIINEEEIVILDAALAASHSILPSVIRSV-SPSQMIA 81

Query: 64  NLFNTRSIHSITLLSKRGIYSSQPDIEDLGKIAITQKKIKVNESLLQRFMRNKALAVTDI 123
              +  +I SITL SKR + +                 + + ES L RF RN++L VTD+
Sbjct: 82  G-GSPITIRSITLFSKRKLSAC----------------LYIEESYLHRFRRNQSLGVTDL 126

Query: 124 TRT 127
           T T
Sbjct: 142 TGT 126

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902050.14.0e-19988.75LOW QUALITY PROTEIN: exonuclease V, chloroplastic [Benincasa hispida][more]
XP_022985622.14.3e-19385.57exonuclease V, chloroplastic [Cucurbita maxima] >XP_022985623.1 exonuclease V, c... [more]
XP_023511975.15.7e-19386.06LOW QUALITY PROTEIN: exonuclease V, chloroplastic [Cucurbita pepo subsp. pepo][more]
KAG6570766.14.8e-19285.57Exonuclease V, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022943527.11.4e-19185.33LOW QUALITY PROTEIN: exonuclease V, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FKK63.0e-9649.88Exonuclease V, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g60370 PE=2 S... [more]
A2VDX71.7e-2728.39Exonuclease V OS=Bos taurus OX=9913 GN=EXO5 PE=2 SV=2[more]
Q9H7901.4e-2428.66Exonuclease V OS=Homo sapiens OX=9606 GN=EXO5 PE=1 SV=1[more]
Q9CXP98.9e-2428.14Exonuclease V OS=Mus musculus OX=10090 GN=Exo5 PE=2 SV=1[more]
F1Q5143.4e-2328.19Exonuclease V OS=Danio rerio OX=7955 GN=exo5 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1J5D72.1e-19385.57exonuclease V, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483632 PE=3 SV... [more]
A0A6J1FT996.7e-19285.33LOW QUALITY PROTEIN: exonuclease V, chloroplastic-like OS=Cucurbita moschata OX=... [more]
A0A5A7SXN18.8e-19288.03Exonuclease V OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00520 P... [more]
A0A0A0KC561.1e-18986.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G290860 PE=3 SV=1[more]
A0A1S3CLU81.1e-18987.28LOW QUALITY PROTEIN: exonuclease V, chloroplastic OS=Cucumis melo OX=3656 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT5G60370.12.1e-9749.88FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT3G57110.12.3e-0634.15unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011604Exonuclease, phage-type/RecB, C-terminalGENE3D3.90.320.10coord: 95..399
e-value: 1.0E-5
score: 27.5
IPR019190Exonuclease VPFAMPF09810Exo5coord: 328..396
e-value: 2.4E-12
score: 47.0
coord: 238..302
e-value: 1.9E-10
score: 40.8
coord: 107..229
e-value: 9.2E-32
score: 110.9
IPR019190Exonuclease VPANTHERPTHR14464EXONUCLEASE Vcoord: 14..402
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10001394.1HG10001394.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0036297 interstrand cross-link repair
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
cellular_component GO:0005634 nucleus
molecular_function GO:0045145 single-stranded DNA 5'-3' exodeoxyribonuclease activity