HG10000516 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10000516
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	ribosome-binding factor A family protein
Location	Chr09: 6132557 .. 6136321 (-)
RNA-Seq Expression	HG10000516
Synteny	HG10000516

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGCATCTTCTGCAGCAAATTCATCCCCAACCCCATATTTCGCCATTGAAACTCTCATCTTCCTTACCATCTTCACCATTTCACTCTCAATTTCAAGCTTCCCGCCCATGGTTTTACTCCCCAAGTTCTTTATCCCTTCTTCGTCCACGTCCGGTGATGAGAGGCGCAACCATACGGTGTATGGCCAATTCACGGAGGGTTAAAATGGTGGCCAAGCAGATTCAGAGGGAGCTTTCCGATATGCTTCTTACCGATAAGGTGTTGCAGTACGCCATTCTTCCTGAGGCTTCTTTGGGAGCTGATAAGTATCTCTCGTCTCTCACCACCATCACCGATGTTGAAGTTTCTGCCGACTTGCAGGTATTTTGAGAGATTGTTCTAATTGAAGAGCTGGGTTGGGTTACTTTTTGCTGTTCTAGCAGAATTAGGTTTTGTTTTTCTTTTGTGCTTTGTTGCCATGAAATTGTAGTTACATTTGTGGAACTGGGAAAGAATTGAAGCTAAGGGCTAAATCCTAAAGGCTGATTGTTATACTTGAATTGGATGGTAAATATTGCATTTGTTGAAGAGAAGGGATTCTGTCAAGTTTTGACTTCTTATTGCTACACAACTCAATTACTGGTTGCATCTTGGATTGTGTGTATAGTGTAGTTTAGTCACTGGGAAATGATTAGGAGACTTCAGAAGCCCATCTGCAAATGTTTGGTTGAAGACATTTTTGATATTGTTATCTTTGGAGCACAAATGAAGAAGTATTCAAATTTTTTAACTCCGAGGATTAAACTATTGGGGTACATATTGAATTGAAGAAAAATTTTGAGCTAAGAAGCACATACACTTTAGTTTGGCTAGTGTGTCCGTGTCGGATCCGACACTTTTTGGACATGGATTGGACACTTATTTGTGCAACAATGTGTTAGGAATGCATAGAACACTTGTTGAGTTGACTAAAAAGATAGATATATTACAATAGTAATAAAATTTGAGTGTGAAATACATCAAACTGATTTTTTAAACATATAAATGCACCAAGTCGTTTGCTTTGAATTTTCTTCTAGTACAAAAATGATATATATTTTTTAAAATGTATATTTTAATAAACGTGTCAGTGTCAAGTCGTGTCCTAAATTTTTAAAAATTGTCGTGTCGTATTTGTGTCTTGTTTTCGTATATTTGTACATCTTAGCTCTTGAGGAATGCTGGGAAGTTACTTTAGCCTGGATAAATAAGTGCTGGGACAACAATGCTTTTGGAAGAATTCCTGGGAAGTTATGCTGACATCAACCACTGGATAAACCATTGCTTCTCAAGCCTGAACTGATTCTACTATCGAGACCAATGAAAGAAGTTCACTGATATCTTTTTTCATAAAGCAACCAATCCAGATCACTTCTGCATTTGAACTTGCAGTGGTAGTTGTGTAAATGGTGGCCTTTTTTTTTTCCCGATATCCGTGAGTGTCCATGTCAGCTTATGCACACCCCGACTAATCTCACGGGACAACCCGCCTGACCCTTAACATTTGGGTGTCAAGAAAACTCGTAGCAAAATTAATTTCCAGGTAGGTGGCCACCGTGGATTGAACCTTTGACCTCTTAGCCATTTATTGAGACTATGTCTCCATTTTACCACTAAGCCAACCCATGATGGTTTGGCTATTTCTTTTTATTGATGTTTGAACAGATTTAACATTGAGTGAGCTGCCTATATCTTAGGATTTTTCTTTTTGTCTTATTTTAATTCAAAATATTTCTTAAACTGAGGTTCAACTATGTTACACCTGTATCTGAGCCTTGACTGTGCTGTTTAGTGGTTTGCTCTTTTTCGACAGTGGCAAACCTCTTGACTTTCAATCTTGCCTAGAAATCAGTTTTCATGCTCTCCATTACGTGATTGTAGTCGATGTCAAATGCAACATAGAGATTATTTCTTACAAGAGTGAACATATACCTTTTTATTATTGTGAAACTTTGAGTACTATGAAGGTCAAACCTTATTTTCGCCATTTAACTCTTCACCTTCTATGAGTTATCAGTTTGTTTGTTATTTAAATTGTACAACTTAGGTACCATGTGGATGTGATGTGAATATGTTAATCTCAACTTAAAATCACCTAGAACAAAAAATAAAGAGCTTCTCCTATTAATTCTGGTGTATATGTTGGCCGCTTTGCCTGATGCTTCAGTTGATAAGTAATGGTTGCACACTATTATATGAATACAAACAAACAGGTGGTTAAAGTATATGTATCGGTCTTTGGCGATGAAAGAGGAAAAGATGTTGCTATTGCCGGGTTGAAGTCTAAAGCTAAGTATGTTCGAAGTGTATTGGGAAAACGTATGAAGCTTCGGCTAACTCCTGAGATTCGCTTTATAGAAGATGAATCTTTTGAGAGAGGAAGCAGGGTAAGTAACAAACTAACATTTTATGTTCTTTATTCTTGTATCAATTTTCTTGCCGTGGCCGGAGACCAGTTTCAATTTTTTTTAAGAATGCCTTTCACAAGATGTTTTCATCTCATATTATTCACTGTTTCCCAATGCACGTGTTTGTGTCTTACAAACTTCAGGTTTATCTCCTATTTCCTATTGAAATATTCCTGGGAAGCTCTAGAAATAATCATTTCTTTGGCTGCTATTTCCTACAACAAATACTTGGAAATGACAAATTTACCAGTGATAAAAAGAGAGATGGTTTTGTGAGAGAAATGGGTCATCGTAGTAATCAGGGCTCGAAATGTTGGAGCTTGGTTCTGGGTTTAAGTTTCTAGCCATAAAACTTAAAAGTGTAAACTCTTGTTGTCTTGAAGTTGACCAGGGGTGGGCTAAAGAAGCACAAATATGGATATGACACAACACAGACATGACAACACACCATTTTTTTAAAATCTAGGACACGACACAGAAATGACACGTTTATTAAAACATATATCATTTTTATACCAGAAGAAAATTCAAAGTAAGTGGGTTGATGCATTTATATGCTTAAAAAATTAGTTTGATGTATTTCACACTCAAAATTTATTATTATGGTCATATATGTGTCTTTTTCATTTACTCAACAAGTGTTTTATGCATGTCTAACACATTGATTGCACTAACAAGTGTCCAATATGTGTCCAACAAGTGTCGGAGTGTCTAAGTGTCTGACACATGTCGAACACGGACACACTAGCAAACTAAATTGCCCGTGCTTCTTAGGGGGTGGGAAAGTAGCTCCAGATTAGTGAATTTGGGTCATAAACATAATGCAATGAGTGGAGGGGTCTTGAAACTTCAGTAGTTCAGTTATCGAGAGAGAGAACAAGATTATGAAGAGTACTGATTGTGGAATAGATTGACTGTTAAAAAGTATAGCACAAACACTCTTATGCATTGGTTAGAATCTTCTTGGGTGGAAAAAATATTCCTGTTTAAAACTCATCCAACCATTGCCTGTTGCAATTTTTCAAGTTTTCACATCCCTATTATTTAAACAACTGGAAGATTACAACAAAGTTCTTTCCTGTACTAATTTGGCTTGAACTTTCCCAGGTAATTGCCATATTGGATCGGATCAAGGACAAAAAGAAGGATACCGATGATGAAGACTTCGAGCCATCTGAACTATCTGATACTCTTGAAGACAACAGGGACTGGAAAGGTGATGATGCTGATGAAGACATAATATATGTACAATAGGTCACTCCATTTCTTGTCCAACTCATGCTCATCTGTGCTGAGGCATCGGTTTGCTCTCGCAGGTTCGTCATCTCGAGTTTAA

mRNA sequence

ATGCCGCATCTTCTGCAGCAAATTCATCCCCAACCCCATATTTCGCCATTGAAACTCTCATCTTCCTTACCATCTTCACCATTTCACTCTCAATTTCAAGCTTCCCGCCCATGGTTTTACTCCCCAAGTTCTTTATCCCTTCTTCGTCCACGTCCGGTGATGAGAGGCGCAACCATACGGTGTATGGCCAATTCACGGAGGGTTAAAATGGTGGCCAAGCAGATTCAGAGGGAGCTTTCCGATATGCTTCTTACCGATAAGGTGTTGCAGTACGCCATTCTTCCTGAGGCTTCTTTGGGAGCTGATAAGTATCTCTCGTCTCTCACCACCATCACCGATGTTGAAGTTTCTGCCGACTTGCAGGTGGTTAAAGTATATGTATCGGTCTTTGGCGATGAAAGAGGAAAAGATGTTGCTATTGCCGGGTTGAAGTCTAAAGCTAAGTATGTTCGAAGTGTATTGGGAAAACGTATGAAGCTTCGGCTAACTCCTGAGATTCGCTTTATAGAAGATGAATCTTTTGAGAGAGGAAGCAGGGTAATTGCCATATTGGATCGGATCAAGGACAAAAAGAAGGATACCGATGATGAAGACTTCGAGCCATCTGAACTATCTGATACTCTTGAAGACAACAGGGACTGGAAAGGTTCGTCATCTCGAGTTTAA

Coding sequence (CDS)

ATGCCGCATCTTCTGCAGCAAATTCATCCCCAACCCCATATTTCGCCATTGAAACTCTCATCTTCCTTACCATCTTCACCATTTCACTCTCAATTTCAAGCTTCCCGCCCATGGTTTTACTCCCCAAGTTCTTTATCCCTTCTTCGTCCACGTCCGGTGATGAGAGGCGCAACCATACGGTGTATGGCCAATTCACGGAGGGTTAAAATGGTGGCCAAGCAGATTCAGAGGGAGCTTTCCGATATGCTTCTTACCGATAAGGTGTTGCAGTACGCCATTCTTCCTGAGGCTTCTTTGGGAGCTGATAAGTATCTCTCGTCTCTCACCACCATCACCGATGTTGAAGTTTCTGCCGACTTGCAGGTGGTTAAAGTATATGTATCGGTCTTTGGCGATGAAAGAGGAAAAGATGTTGCTATTGCCGGGTTGAAGTCTAAAGCTAAGTATGTTCGAAGTGTATTGGGAAAACGTATGAAGCTTCGGCTAACTCCTGAGATTCGCTTTATAGAAGATGAATCTTTTGAGAGAGGAAGCAGGGTAATTGCCATATTGGATCGGATCAAGGACAAAAAGAAGGATACCGATGATGAAGACTTCGAGCCATCTGAACTATCTGATACTCTTGAAGACAACAGGGACTGGAAAGGTTCGTCATCTCGAGTTTAA

Protein sequence

MPHLLQQIHPQPHISPLKLSSSLPSSPFHSQFQASRPWFYSPSSLSLLRPRPVMRGATIRCMANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSADLQVVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRVIAILDRIKDKKKDTDDEDFEPSELSDTLEDNRDWKGSSSRV

Homology

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BLAST of HG10000516 vs. NCBI nr
Match: XP_038900542.1 (probable ribosome-binding factor A, chloroplastic [Benincasa hispida])

HSP 1 Score: 387.9 bits (995), Expect = 5.9e-104
Identity = 207/219 (94.52%), Postives = 212/219 (96.80%), Query Frame = 0


Query: 1   MPHLLQQIHPQPHISPLKLSSSLPSSPFHSQFQASRPWFYSPSSLSLLRPRPVMRGATIR 60
           MPHLLQQIHPQPH+SPLKLSSSLPSSPF SQFQASRPWFYSPSS++LLRP P M GATIR
Sbjct: 1   MPHLLQQIHPQPHLSPLKLSSSLPSSPFPSQFQASRPWFYSPSSVTLLRP-PRMMGATIR 60

Query: 61  CMANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSADL 120
           CMANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSADL
Sbjct: 61  CMANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSADL 120

Query: 121 QVVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV 180
           QVVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV
Sbjct: 121 QVVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV 180

Query: 181 IAILDRIKDKKKDTDDEDFEPSELSDTLEDNRDWKGSSS 220
           IAILDRIKDKKKD DDEDFEPSELSDTLED+RDWKG  +
Sbjct: 181 IAILDRIKDKKKDNDDEDFEPSELSDTLEDDRDWKGDDA 218

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BLAST of HG10000516 vs. NCBI nr
Match: XP_008457619.1 (PREDICTED: probable ribosome-binding factor A, chloroplastic [Cucumis melo] >XP_016902146.1 PREDICTED: probable ribosome-binding factor A, chloroplastic [Cucumis melo] >KAA0045661.1 putative ribosome-binding factor A [Cucumis melo var. makuwa] >TYK02601.1 putative ribosome-binding factor A [Cucumis melo var. makuwa])

HSP 1 Score: 354.8 bits (909), Expect = 5.5e-94
Identity = 194/220 (88.18%), Postives = 204/220 (92.73%), Query Frame = 0


Query: 1   MPH-LLQQIHPQPHISPLKLSSSLPSSPFHSQFQASRPWFYSPSSLSLLRPRPVMRGATI 60
           MPH LLQQIHPQ + SPLK SSSL SSPF SQFQASRP FYSPSS++LL P PVMRGATI
Sbjct: 1   MPHLLLQQIHPQFYFSPLKFSSSLQSSPFPSQFQASRPCFYSPSSVNLLPPPPVMRGATI 60

Query: 61  RCMANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSAD 120
           RCMANSRRVKMVAKQIQRELSDMLLTDKVLQ+AILPEASLGADKYLSSLTTITDVEVSAD
Sbjct: 61  RCMANSRRVKMVAKQIQRELSDMLLTDKVLQFAILPEASLGADKYLSSLTTITDVEVSAD 120

Query: 121 LQVVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSR 180
           LQVVKVYVSVFGDE+GKDVA+AGLKSKAKYVRSVLGKRMKLRLTPEI FIEDESFERGSR
Sbjct: 121 LQVVKVYVSVFGDEKGKDVAMAGLKSKAKYVRSVLGKRMKLRLTPEICFIEDESFERGSR 180

Query: 181 VIAILDRIKDKKKDTDDEDFEPSELSDTLEDNRDWKGSSS 220
           VIAILDRIKDKKKDT DEDF+  ELSD L+D+RDWKG  +
Sbjct: 181 VIAILDRIKDKKKDTIDEDFDEPELSDALDDDRDWKGDDA 220

[Close]

BLAST of HG10000516 vs. NCBI nr
Match: XP_004151338.1 (probable ribosome-binding factor A, chloroplastic [Cucumis sativus] >KGN61860.1 hypothetical protein Csa_006608 [Cucumis sativus])

HSP 1 Score: 343.2 bits (879), Expect = 1.7e-90
Identity = 188/216 (87.04%), Postives = 198/216 (91.67%), Query Frame = 0


Query: 4   LLQQIHPQPHISPLKLSSSLPSSPFHSQFQASRPWFYSPSSLSLLRPRPVMRGATIRCMA 63
           LLQQIHPQ + SPLK    L SSPF SQFQASRP  YSP+S++LL P PVMRGATIRCMA
Sbjct: 5   LLQQIHPQFYFSPLKF---LQSSPFPSQFQASRPCVYSPTSVTLLPPPPVMRGATIRCMA 64

Query: 64  NSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSADLQVV 123
           NSRR+KMVAKQIQRELSDMLLTDKVLQ+AILPEASLGADKYLSSLTTITDVEVSADLQVV
Sbjct: 65  NSRRIKMVAKQIQRELSDMLLTDKVLQFAILPEASLGADKYLSSLTTITDVEVSADLQVV 124

Query: 124 KVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRVIAI 183
           KVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRVIAI
Sbjct: 125 KVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRVIAI 184

Query: 184 LDRIKDKKKDTDDEDFEPSELSDTLEDNRDWKGSSS 220
           LDRIKDKKKDT DEDF+  ELSD L+D+RDWKG  +
Sbjct: 185 LDRIKDKKKDTVDEDFDQPELSDALDDDRDWKGDDA 217

[Close]

BLAST of HG10000516 vs. NCBI nr
Match: XP_023006992.1 (probable ribosome-binding factor A, chloroplastic [Cucurbita maxima])

HSP 1 Score: 337.8 bits (865), Expect = 7.0e-89
Identity = 181/215 (84.19%), Postives = 198/215 (92.09%), Query Frame = 0


Query: 1   MPHLLQQIHPQPHISPLKLSSSLPSSPFHSQFQASRPWFYSPSSLSLLRPRPVMRGATIR 60
           MPHLLQQIHPQPH  PLKLSSSL SS F SQF  S P F SP+S++L+R RPVMRGATI+
Sbjct: 1   MPHLLQQIHPQPHHFPLKLSSSLASSRFPSQFLTSPPCFCSPNSVALVRQRPVMRGATIQ 60

Query: 61  CMANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSADL 120
           CMANSRRVKMV+KQIQRELS+MLLTDKVLQ+AILPEASLGADKYLSSLTTITDVE+S+DL
Sbjct: 61  CMANSRRVKMVSKQIQRELSNMLLTDKVLQFAILPEASLGADKYLSSLTTITDVEISSDL 120

Query: 121 QVVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV 180
           QVVKVYVSVFGDERGKD+A+AGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV
Sbjct: 121 QVVKVYVSVFGDERGKDIAMAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV 180

Query: 181 IAILDRIKDKKKDTDDEDFEPSELSDTLEDNRDWK 216
           IAILDRIKD+K + DDED E SELSD  +D+RDW+
Sbjct: 181 IAILDRIKDEKNNIDDEDMEKSELSDAPQDDRDWE 215

[Close]

BLAST of HG10000516 vs. NCBI nr
Match: KAG6605037.1 (putative ribosome-binding factor A, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 337.4 bits (864), Expect = 9.2e-89
Identity = 182/215 (84.65%), Postives = 198/215 (92.09%), Query Frame = 0


Query: 1   MPHLLQQIHPQPHISPLKLSSSLPSSPFHSQFQASRPWFYSPSSLSLLRPRPVMRGATIR 60
           MPHLLQQIHPQ H  PLKLSSSL SS F SQF AS P F SP+S++L+R RPVMRGATIR
Sbjct: 1   MPHLLQQIHPQSHHFPLKLSSSLASSRFPSQFLASPPCFCSPNSVALVRRRPVMRGATIR 60

Query: 61  CMANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSADL 120
           CMANSRRVKMV+KQIQRELS+MLLTDKVLQ+AILPEASLGADKYLSSLTTITDVE+S+DL
Sbjct: 61  CMANSRRVKMVSKQIQRELSNMLLTDKVLQFAILPEASLGADKYLSSLTTITDVEISSDL 120

Query: 121 QVVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV 180
           QVVKVYVSVFGDERGKD+A+AGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV
Sbjct: 121 QVVKVYVSVFGDERGKDIAMAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV 180

Query: 181 IAILDRIKDKKKDTDDEDFEPSELSDTLEDNRDWK 216
           IAILDRIKD+K + DDED E SELSD  +D+RDW+
Sbjct: 181 IAILDRIKDEKNNIDDEDMEQSELSDAPQDDRDWE 215

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BLAST of HG10000516 vs. ExPASy Swiss-Prot
Match: O65693 (Probable ribosome-binding factor A, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g34730 PE=2 SV=2)

HSP 1 Score: 227.3 bits (578), Expect = 1.8e-58
Identity = 127/178 (71.35%), Postives = 150/178 (84.27%), Query Frame = 0


Query: 39  FYSPSSLSLLRPRPVMR-GATIRCMANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEA 98
           F+ P S++ +  R  +    T+RCMAN RRVKMVAKQI RELSDMLLTD VLQ+A+LPEA
Sbjct: 30  FHFPQSMAPVNLRTNLSVRRTVRCMANPRRVKMVAKQIMRELSDMLLTDTVLQHAVLPEA 89

Query: 99  SLGADKYLSSLTTITDVEVSADLQVVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKR 158
           +LGAD+YLSSLTTI+DVEVS DLQVVKVYVSVFGD+RGKDVAIAGLKSKAKYVRS LGKR
Sbjct: 90  ALGADRYLSSLTTISDVEVSNDLQVVKVYVSVFGDDRGKDVAIAGLKSKAKYVRSELGKR 149

Query: 159 MKLRLTPEIRFIEDESFERGSRVIAILDRIKDKKKDTDDEDFEPSELSDTLEDNRDWK 216
           MKLRLTPE+RFIEDE+ ERGSRVIAILD+IK +K   + +    +E SD+ ED++DW+
Sbjct: 150 MKLRLTPEVRFIEDEAMERGSRVIAILDKIKAEKGSGEGK----TEPSDSTEDDQDWE 203

[Close]

BLAST of HG10000516 vs. ExPASy Swiss-Prot
Match: B0JRP8 (Ribosome-binding factor A OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=rbfA PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 5.0e-21
Identity = 58/139 (41.73%), Postives = 88/139 (63.31%), Query Frame = 0


Query: 62  MANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSADLQ 121
           MAN RRV  V+  I+RE+S MLL +            +  D+  + + ++TDV++S DLQ
Sbjct: 1   MANDRRVSRVSSLIKREVSQMLLME------------IKDDRVGAGMVSVTDVDLSHDLQ 60

Query: 122 VVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRVI 181
             K++VS++G+E  K   +AGLK+ A +VR  LG+R++LR TPE+ F+ED S ERG R++
Sbjct: 61  HAKIFVSIYGNEDAKAETMAGLKASAGFVRRELGQRIRLRRTPEVVFLEDSSLERGDRML 120

Query: 182 AILDRIKDKKKDTDDEDFE 201
            +LD IK  +   DD+  E
Sbjct: 121 HLLDHIKTDRPPEDDDSEE 127

[Close]

BLAST of HG10000516 vs. ExPASy Swiss-Prot
Match: Q8Z0M8 (Ribosome-binding factor A OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=rbfA PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 5.0e-21
Identity = 56/134 (41.79%), Postives = 86/134 (64.18%), Query Frame = 0


Query: 62  MANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSADLQ 121
           MA +RRV  VA+ I+RE+S ML+              +  D+  + + ++TDV+VS DLQ
Sbjct: 1   MATNRRVSRVAELIKREVSQMLIN------------GIKDDRVGTGMVSVTDVDVSGDLQ 60

Query: 122 VVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRVI 181
             K+YVS++G E  K   +AGLKS   +VRS LG R++LR TPE+ FIED S ERG++V+
Sbjct: 61  HAKIYVSIYGTEEAKAETMAGLKSATGFVRSELGARVRLRRTPEVTFIEDRSIERGTKVL 120

Query: 182 AILDRIKDKKKDTD 196
            +L+++++ +   D
Sbjct: 121 TLLNKLENARSPDD 122

[Close]

BLAST of HG10000516 vs. ExPASy Swiss-Prot
Match: Q3M9Q8 (Ribosome-binding factor A OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=rbfA PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.1e-20
Identity = 56/134 (41.79%), Postives = 86/134 (64.18%), Query Frame = 0


Query: 62  MANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSADLQ 121
           MA +RRV  VA+ I+RE+S ML+              +  D+  + + ++TDV+VS DLQ
Sbjct: 1   MATNRRVSRVAELIKREVSQMLIN------------GIKDDRVGTGMVSVTDVDVSGDLQ 60

Query: 122 VVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRVI 181
             K+YVS++G E  K   +AGLKS   +VRS LG R++LR TPE+ FIED S ERG++V+
Sbjct: 61  HAKIYVSIYGTEEAKAETMAGLKSATGFVRSELGARVRLRRTPEVTFIEDRSIERGTKVL 120

Query: 182 AILDRIKDKKKDTD 196
            +L+++++ +   D
Sbjct: 121 TLLNKLENARSLDD 122

[Close]

BLAST of HG10000516 vs. ExPASy Swiss-Prot
Match: Q93LL7 (Ribosome-binding factor A OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) OX=63737 GN=rbfA PE=3 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.9e-20
Identity = 61/147 (41.50%), Postives = 89/147 (60.54%), Query Frame = 0


Query: 62  MANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSADLQ 121
           MA +RRV  VA+ I+RE+S MLL              +  D+  + + ++TDV+VS DLQ
Sbjct: 1   MATNRRVSRVAELIKREVSQMLLN------------GIKDDRVGTGMVSVTDVDVSGDLQ 60

Query: 122 VVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRVI 181
             K+YVS++G +  K   +AGLKS   YVRS LG R++LR TPE+ F+ED S ERG++V+
Sbjct: 61  HAKIYVSIYGTDEAKVETMAGLKSATGYVRSELGARVRLRRTPEVIFLEDRSIERGNKVL 120

Query: 182 AILDRIKDKK--------KDTDDEDFE 201
           A+L ++   +        +D  DED E
Sbjct: 121 ALLSQLNHDRPPENLLAVEDNTDEDDE 135

[Close]

BLAST of HG10000516 vs. ExPASy TrEMBL
Match: A0A1S4E1P3 (probable ribosome-binding factor A, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103497269 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 2.7e-94
Identity = 194/220 (88.18%), Postives = 204/220 (92.73%), Query Frame = 0


Query: 1   MPH-LLQQIHPQPHISPLKLSSSLPSSPFHSQFQASRPWFYSPSSLSLLRPRPVMRGATI 60
           MPH LLQQIHPQ + SPLK SSSL SSPF SQFQASRP FYSPSS++LL P PVMRGATI
Sbjct: 1   MPHLLLQQIHPQFYFSPLKFSSSLQSSPFPSQFQASRPCFYSPSSVNLLPPPPVMRGATI 60

Query: 61  RCMANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSAD 120
           RCMANSRRVKMVAKQIQRELSDMLLTDKVLQ+AILPEASLGADKYLSSLTTITDVEVSAD
Sbjct: 61  RCMANSRRVKMVAKQIQRELSDMLLTDKVLQFAILPEASLGADKYLSSLTTITDVEVSAD 120

Query: 121 LQVVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSR 180
           LQVVKVYVSVFGDE+GKDVA+AGLKSKAKYVRSVLGKRMKLRLTPEI FIEDESFERGSR
Sbjct: 121 LQVVKVYVSVFGDEKGKDVAMAGLKSKAKYVRSVLGKRMKLRLTPEICFIEDESFERGSR 180

Query: 181 VIAILDRIKDKKKDTDDEDFEPSELSDTLEDNRDWKGSSS 220
           VIAILDRIKDKKKDT DEDF+  ELSD L+D+RDWKG  +
Sbjct: 181 VIAILDRIKDKKKDTIDEDFDEPELSDALDDDRDWKGDDA 220

[Close]

BLAST of HG10000516 vs. ExPASy TrEMBL
Match: A0A5A7TVW4 (Putative ribosome-binding factor A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold280G00030 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 2.7e-94
Identity = 194/220 (88.18%), Postives = 204/220 (92.73%), Query Frame = 0


Query: 1   MPH-LLQQIHPQPHISPLKLSSSLPSSPFHSQFQASRPWFYSPSSLSLLRPRPVMRGATI 60
           MPH LLQQIHPQ + SPLK SSSL SSPF SQFQASRP FYSPSS++LL P PVMRGATI
Sbjct: 1   MPHLLLQQIHPQFYFSPLKFSSSLQSSPFPSQFQASRPCFYSPSSVNLLPPPPVMRGATI 60

Query: 61  RCMANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSAD 120
           RCMANSRRVKMVAKQIQRELSDMLLTDKVLQ+AILPEASLGADKYLSSLTTITDVEVSAD
Sbjct: 61  RCMANSRRVKMVAKQIQRELSDMLLTDKVLQFAILPEASLGADKYLSSLTTITDVEVSAD 120

Query: 121 LQVVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSR 180
           LQVVKVYVSVFGDE+GKDVA+AGLKSKAKYVRSVLGKRMKLRLTPEI FIEDESFERGSR
Sbjct: 121 LQVVKVYVSVFGDEKGKDVAMAGLKSKAKYVRSVLGKRMKLRLTPEICFIEDESFERGSR 180

Query: 181 VIAILDRIKDKKKDTDDEDFEPSELSDTLEDNRDWKGSSS 220
           VIAILDRIKDKKKDT DEDF+  ELSD L+D+RDWKG  +
Sbjct: 181 VIAILDRIKDKKKDTIDEDFDEPELSDALDDDRDWKGDDA 220

[Close]

BLAST of HG10000516 vs. ExPASy TrEMBL
Match: A0A0A0LIX7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G252090 PE=3 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 8.1e-91
Identity = 188/216 (87.04%), Postives = 198/216 (91.67%), Query Frame = 0


Query: 4   LLQQIHPQPHISPLKLSSSLPSSPFHSQFQASRPWFYSPSSLSLLRPRPVMRGATIRCMA 63
           LLQQIHPQ + SPLK    L SSPF SQFQASRP  YSP+S++LL P PVMRGATIRCMA
Sbjct: 5   LLQQIHPQFYFSPLKF---LQSSPFPSQFQASRPCVYSPTSVTLLPPPPVMRGATIRCMA 64

Query: 64  NSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSADLQVV 123
           NSRR+KMVAKQIQRELSDMLLTDKVLQ+AILPEASLGADKYLSSLTTITDVEVSADLQVV
Sbjct: 65  NSRRIKMVAKQIQRELSDMLLTDKVLQFAILPEASLGADKYLSSLTTITDVEVSADLQVV 124

Query: 124 KVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRVIAI 183
           KVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRVIAI
Sbjct: 125 KVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRVIAI 184

Query: 184 LDRIKDKKKDTDDEDFEPSELSDTLEDNRDWKGSSS 220
           LDRIKDKKKDT DEDF+  ELSD L+D+RDWKG  +
Sbjct: 185 LDRIKDKKKDTVDEDFDQPELSDALDDDRDWKGDDA 217

[Close]

BLAST of HG10000516 vs. ExPASy TrEMBL
Match: A0A6J1KXB5 (probable ribosome-binding factor A, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499618 PE=3 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 3.4e-89
Identity = 181/215 (84.19%), Postives = 198/215 (92.09%), Query Frame = 0


Query: 1   MPHLLQQIHPQPHISPLKLSSSLPSSPFHSQFQASRPWFYSPSSLSLLRPRPVMRGATIR 60
           MPHLLQQIHPQPH  PLKLSSSL SS F SQF  S P F SP+S++L+R RPVMRGATI+
Sbjct: 1   MPHLLQQIHPQPHHFPLKLSSSLASSRFPSQFLTSPPCFCSPNSVALVRQRPVMRGATIQ 60

Query: 61  CMANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSADL 120
           CMANSRRVKMV+KQIQRELS+MLLTDKVLQ+AILPEASLGADKYLSSLTTITDVE+S+DL
Sbjct: 61  CMANSRRVKMVSKQIQRELSNMLLTDKVLQFAILPEASLGADKYLSSLTTITDVEISSDL 120

Query: 121 QVVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV 180
           QVVKVYVSVFGDERGKD+A+AGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV
Sbjct: 121 QVVKVYVSVFGDERGKDIAMAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV 180

Query: 181 IAILDRIKDKKKDTDDEDFEPSELSDTLEDNRDWK 216
           IAILDRIKD+K + DDED E SELSD  +D+RDW+
Sbjct: 181 IAILDRIKDEKNNIDDEDMEKSELSDAPQDDRDWE 215

[Close]

BLAST of HG10000516 vs. ExPASy TrEMBL
Match: A0A6J1G8H2 (probable ribosome-binding factor A, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111451795 PE=3 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 4.4e-89
Identity = 182/215 (84.65%), Postives = 198/215 (92.09%), Query Frame = 0


Query: 1   MPHLLQQIHPQPHISPLKLSSSLPSSPFHSQFQASRPWFYSPSSLSLLRPRPVMRGATIR 60
           MPHLLQQIHPQ H  PLKLSSSL SS F SQF AS P F SP+S++L+R RPVMRGATIR
Sbjct: 1   MPHLLQQIHPQSHHFPLKLSSSLASSRFPSQFLASPPCFCSPNSVALVRRRPVMRGATIR 60

Query: 61  CMANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEASLGADKYLSSLTTITDVEVSADL 120
           CMANSRRVKMV+KQIQRELS+MLLTDKVLQ+AILPEASLGADKYLSSLTTITDVE+S+DL
Sbjct: 61  CMANSRRVKMVSKQIQRELSNMLLTDKVLQFAILPEASLGADKYLSSLTTITDVEISSDL 120

Query: 121 QVVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV 180
           QVVKVYVSVFGDERGKD+A+AGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV
Sbjct: 121 QVVKVYVSVFGDERGKDIAMAGLKSKAKYVRSVLGKRMKLRLTPEIRFIEDESFERGSRV 180

Query: 181 IAILDRIKDKKKDTDDEDFEPSELSDTLEDNRDWK 216
           IAILDRIKD+K + DDED E SELSD  +D+RDW+
Sbjct: 181 IAILDRIKDEKNNIDDEDMEQSELSDAPQDDRDWE 215

[Close]

BLAST of HG10000516 vs. TAIR 10
Match: AT4G34730.1 (ribosome-binding factor A family protein )

HSP 1 Score: 227.3 bits (578), Expect = 1.2e-59
Identity = 127/178 (71.35%), Postives = 150/178 (84.27%), Query Frame = 0


Query: 39  FYSPSSLSLLRPRPVMR-GATIRCMANSRRVKMVAKQIQRELSDMLLTDKVLQYAILPEA 98
           F+ P S++ +  R  +    T+RCMAN RRVKMVAKQI RELSDMLLTD VLQ+A+LPEA
Sbjct: 30  FHFPQSMAPVNLRTNLSVRRTVRCMANPRRVKMVAKQIMRELSDMLLTDTVLQHAVLPEA 89

Query: 99  SLGADKYLSSLTTITDVEVSADLQVVKVYVSVFGDERGKDVAIAGLKSKAKYVRSVLGKR 158
           +LGAD+YLSSLTTI+DVEVS DLQVVKVYVSVFGD+RGKDVAIAGLKSKAKYVRS LGKR
Sbjct: 90  ALGADRYLSSLTTISDVEVSNDLQVVKVYVSVFGDDRGKDVAIAGLKSKAKYVRSELGKR 149

Query: 159 MKLRLTPEIRFIEDESFERGSRVIAILDRIKDKKKDTDDEDFEPSELSDTLEDNRDWK 216
           MKLRLTPE+RFIEDE+ ERGSRVIAILD+IK +K   + +    +E SD+ ED++DW+
Sbjct: 150 MKLRLTPEVRFIEDEAMERGSRVIAILDKIKAEKGSGEGK----TEPSDSTEDDQDWE 203

The following BLAST results are available for this feature:

BLAST of HG10000516 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900542.1	5.9e-104	94.52	probable ribosome-binding factor A, chloroplastic [Benincasa hispida]	[more]
XP_008457619.1	5.5e-94	88.18	PREDICTED: probable ribosome-binding factor A, chloroplastic [Cucumis melo] >XP_...	[more]
XP_004151338.1	1.7e-90	87.04	probable ribosome-binding factor A, chloroplastic [Cucumis sativus] >KGN61860.1 ...	[more]
XP_023006992.1	7.0e-89	84.19	probable ribosome-binding factor A, chloroplastic [Cucurbita maxima]	[more]
KAG6605037.1	9.2e-89	84.65	putative ribosome-binding factor A, chloroplastic, partial [Cucurbita argyrosper...	[more]

BLAST of HG10000516 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65693	1.8e-58	71.35	Probable ribosome-binding factor A, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
B0JRP8	5.0e-21	41.73	Ribosome-binding factor A OS=Microcystis aeruginosa (strain NIES-843) OX=449447 ...	[more]
Q8Z0M8	5.0e-21	41.79	Ribosome-binding factor A OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576...	[more]
Q3M9Q8	1.1e-20	41.79	Ribosome-binding factor A OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937...	[more]
Q93LL7	1.9e-20	41.50	Ribosome-binding factor A OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) ...	[more]

BLAST of HG10000516 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4E1P3	2.7e-94	88.18	probable ribosome-binding factor A, chloroplastic OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A5A7TVW4	2.7e-94	88.18	Putative ribosome-binding factor A OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A0A0LIX7	8.1e-91	87.04	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G252090 PE=3 SV=1	[more]
A0A6J1KXB5	3.4e-89	84.19	probable ribosome-binding factor A, chloroplastic OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1G8H2	4.4e-89	84.65	probable ribosome-binding factor A, chloroplastic OS=Cucurbita moschata OX=3662 ...	[more]

BLAST of HG10000516 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G34730.1	1.2e-59	71.35	ribosome-binding factor A family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000238	Ribosome-binding factor A	PFAM	PF02033	RBFA	coord: 67..184 e-value: 1.4E-27 score: 96.2
IPR000238	Ribosome-binding factor A	TIGRFAM	TIGR00082	TIGR00082	coord: 62..188 e-value: 8.7E-24 score: 82.2
IPR000238	Ribosome-binding factor A	PANTHER	PTHR33515	RIBOSOME-BINDING FACTOR A, CHLOROPLASTIC-RELATED	coord: 55..216
IPR000238	Ribosome-binding factor A	HAMAP	MF_00003	RbfA	coord: 66..184 score: 22.110432
IPR015946	K homology domain-like, alpha/beta	GENE3D	3.30.300.20		coord: 62..178 e-value: 2.1E-35 score: 123.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 205..221
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 190..221
IPR020053	Ribosome-binding factor A, conserved site	PROSITE	PS01319	RBFA	coord: 150..171
IPR023799	Ribosome-binding factor A domain superfamily	SUPERFAMILY	89919	Ribosome-binding factor A, RbfA	coord: 62..187

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10000516.1	HG10000516.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006364	rRNA processing
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0043024	ribosomal small subunit binding