HG10000464 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000464
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionisoflavone reductase homolog
LocationChr09: 5479248 .. 5484911 (+)
RNA-Seq ExpressionHG10000464
SyntenyHG10000464
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGTTTTGATCTTGATACAACAAAAACAGAGTGGAGAAGCATCATCTTCTTTGCATTTGCAGCTCACCGGTACAGCTCCGATCAAGCATTGTTCGCCGGAAACCATAATCCGATGACTCAGAAGCTCAAGGTTCTCATCATCGCAACTGGTTACATCGGAATTTTTTTTGTAGAAGCGAGCGTCAAAGCAGGTAAAAAAATTGCTTATTTATTTATTTTAATTATTATTAAGATGATAGTTAGATAAACAAATGTTAGGTTTAAATTGGTGGTTTGTATCGATTTGGTCCCTAAGCTCTGAATTTAGTTATGTTGATATATTTTAGTAAGAAAATTTAAATTTTTTTTATGCAAAACAAAATAAATGTTACATTCGGAGGAAAGACTAGACAAATTATTTCTTTGTTTTTTTTTTAGTTCAACAATGGTGTGGTACAACCTCCATCCTTGAAATGACGATTAATTTCTTATCTACAAAACTCGACTCAAATTGGCAAATTATTATTTAATTAACATATTTTGATACATTAGCATATGAGTAAGGGACAAATTGACAATAACATAATAAGTTAATGATACCTCACTTTTTGCACTTTTAACCTAATAATTTAAACATATCTGAGTTTTGAATTTCCTCACTCCATAGTGTGGAACTTTAAAAAAAATAATAATAATACACCACTTTTTGTTCATCAAGATTAATATCCTTTGTAAAATGATTTAATTGAAAATCTTACAAGATTAATAGTTACTTCAAATATAGATGCATAAAAAAGTTATAAAGTATAATAGAATCTTAACTTTCAAGTTAATGTTTAATATGTCCTTAAATCTTTAATTTTGTATCTAATAGGTTATTGACCTATTTAATATTTTTCAAAAATTTTAAATTAATATAGAGATAGCAATACGGGGTGGGTAGGGTCGTAGATGTACTCCCCATCTTCGTTTGGATGAATTGGGGTTGGGGTGGGGAATCCTAATTCAGGGATCAGATCCCATGGGAAAAATTTTCTCATATTTTTTTTCTTCTTATCATTAAAAACTAATGAAAGTTTGATAAATACGTGGAAATTATTAGACTATTTTCAAATATAGAAAAATGAGACAAACTATTTACAAATATAAAAAAAAATTACTGTCTATCAGCGATAGACTGCAATAGACTTCTATCGCGGTCTATCGTTCAAACGATATAAGTCTATAGTTGATAGATTGTGAAATTTTTCTTTATTTGTAAATAGTTTGATATTTTTTCTATTTGTTAAATTTAGCATTATTATTATTTTTTATTTAAATAATTATTTCTAACAAATATCTAACACTTTTTAATTATAAGTTTCAATACAAAAAAAATTATTATAACATGATTAAAGTATCATTTTGATCTGTGTACTTTAAGGTTTTTTCAATTTTAGTTTCACTTTGTCTAATTTTATCACTGTATTTTAAATAATTCTTAAATTCAATCCCTCAAACTTCATTTTTATCAAAACTTGCAATATAATTATAATAATTTTTATGCTAAAAAAATATAATGTTTGAATATATTTTCAAATTTACAGTGAAAATATTAATAGAAAACAAAATGTTCTATAATTTTCCATTTCACCTTCCCACTCTGTCAAAATTAGAGATTCCAAATTTTGTCACCCTTACTTAATAAACATAAATTTGGAAGTTTATAGACTTATTTAATACAAATTGTACACAAATTTAAGACTAAACTTCTCATTTTAACTAATTATATGCTAGAACGTAGAAGAAGTGAAGAACTAGAAGTTGGCCATTGTTGACCGAAACAAAGAAAATCACTAATGTGAGGGCAGTGGCGGTGGCGGTTGCGGCGGTCGCCGGCCTTCCCCCACCAACCACATATCTTAGTCGCTGCATCTCTTCTCCATTTTTATTTTTATTCTTTTTTGAAAAGAATCTCCTCCCCATTTGTATTCATTTTAGAGGTTGTTTTGCACGCTGAGTTGAGTTCATATGTTTGTGAAATTTATATATTTGTGGAGTTCATGTGTCTATGACTTGAGTGTTTTGGATGCTGAGTTCATATATGTCGGATGTATCTGGTACAACTTCTTGTACATAAATAATCTGTTTTTTTATGATGATTATTTTTGTGTGGTAACTCAACTAAACAATATTCAACATGGTCTAAAAGAACTACTATACACAAAATAACTCACTAAGGTTTAAAATGTCAGTGTCAATGAAAATATTGAGATCTCAATTTTACGAAATGTTGATGAAAATATTGATAATATGCCAATGTCGATGAATATTTATAAAAAAATATATACAAATATATTTAACTTAGTAAATGAACATTCAACACTTTTCAAATTAATTGTTAAAAAAAATGGTACTAAAAAACTATTTATTATTTATATTATATTCATATTATTTATAGTTTGTTGCTCATTTTTTCTTTTTTATAGATTTTTTACGATATAATGAAAATATTGATTTATCTCTCATGTCATTATCGAATCTATAAAAATTTGAAAATATCGATAACGATACCAATATATGAATAGAAATTTAATATCATGTTCACGTGAAAATATTTAAGTTAATTTCAAATATTAAAAAAAACACTTTTTTTTATATATAAAGAAAAACACACTTAGCCACAATACTTTTTCCCTTTAAAAAGTTTTAGTAAATTCTCGAATTGTCATGATGGTATTATTTATTTGCTATCATTTTTAAACATCTTAAACCATTCATAATCACACTTGTAATTTAAACAATGTTCTTAATTCACAATAGCCAATAAACATCTTATACATTTAGCAATGATTAGATATATACTTAAAGAACTATGTTCGGATTGGCTAGCTTTGTATTTCCTTTCTTTTGTTTTAAAAAAAAATGGTCAGCCTTTGTAGTTTAACTTATGCTAAATTAAAAAATATATGTGTAGAAAAATTTTCACAAATAAAAAAAAATGTCAAACTATTTGCAGAAAATAACAAAAAAAACACTAATAGATAGGGATAGATTTCTATCAATGATAGACCCTAATGGACTTCTATCACCGTTTATCACAACTATCTAAAAATTTTGTTATTTTGTGTAAATAATTTTCTTTATTTCTCTATTTTTAAAAATTCTCCAAAAATATATTCTTGACCTTTTTCTTTCTTTTCAAAAATTTCAATATTATACTCTTAACTTGAAAATTGGAATTTGATATGAGAGTGGTGGTAACCTAAAATAATGAGTCATTTTTCAGGTCTCGACTTTCATTCCAAGTCACTGCTACACCAAGCTTTAATTTTTTTTTTTTTTTTTTTTTTGCTAATATTCTAAAGAAGTTCTATAGATTTAAAATGCTTACAAGCAATTTTCGATAGAATGGAATATTTTTTTTCAACAAATGACAAAGTTTTGTGGGTTTTTTTTTTAAAAAAAGAAATAATTAACATATATAAATTTCAAAAACTATATATATTTCCCACTCATCAACGAAAAACTAGGATCATCTTCTTAGTTCTTTCCATTTGCAGGTCACCGTCACAGCTCCGATCAGGTTTTGTTTGCCGGAAACCTTAATTTGATGGCTCAGAAGCTCAAAGTTCTCATCATCGGAGGTACTGGCTACATCGGAAAATTCCTAGTAGAAGCCAGCGCAAAAGCAGGTCATCCAACTTATATATTGGTCCGCGAATCTACCCTCGCTGCCTCCACTAAGTCTTCGATCATCGATAATTTCAGGAGCTTGGGTGTCAATTTCGTCTTCGTAAGTTTCTTTTTCTCTATCTCTCTGGATTTTGTCGTTTGATTGTTGAGACTGTACAATAAGATTACGGAATCGATTGCTGAAATGTGAAGTGTTCTTCAATTTTCTTGGTTTCCCGAACAACTGAATTAGATCTTTACTTCAATTCTGCGAAGTGAAGATTGTCTGTTTGGTTTTTCTGAAACTAATAATGCAGTATATACTGATGATCCTTTAAGTCCACTCATGCTATAATCTTTGGAAGTGATAATGTTCAATTTGCTAAGACTCTGAGAAATTGGGGCAGGGCGATATCTTCGACAATGAGAGTCTGGTTAGGGCAATACAGCAGGTTGATGTGGTGATATCCACCGTTGGTCGTTCTCTGTTATCCGATCAAGATAAGATCATATCTGCTATCAAACAAGCTGGTAATGTTAAGGTAACCCTTCAACCATTTCACCACCGCCATTCCCTTACTAAATTTGTTCCCTGTTTTGCCATTGATTGTTCTTGCTGCAAGCGTTGTAGTGTTGTAGTCCTAAAGTATTGATATAATTAAATATACCATAAATTATCAACTTAAACTTTTAGGTTGATTATTGATTTAGAGTTCGTAAAACTCAAATTGGTATTCTATCCAAAAAGAAATTGGGATCCTATCCAAGAGTGTTGAATCTAAAAGAGGCCTCACAATGTTCGTAAGATACATGGGCTACTTCTTTCTTCACCAATTAGTTTTGAGATAGAATCTCATGTTATCTTATACGGTATTAGAGACCTTATGTGTTCAAACACCTATAATATCATTTTCTCTCTCATTTTATATTGATTTCCACTTGTTGGATATTTTATAAATTTTCAAGTCAACTGAAGCTTCTAGGGTGATTAGTGATTTAACAAAGATACGCACTGCAAGAGAGTTCGTGTGAGGTTGGGAATTTTTTTGTATTAAAAAATTTGTGTGGTATAGAGATTTTTTCCCTCTGAGTTTGGCAACGATGTGGATCGTGTTCATGCCGTGGAGCCAGCAAAATCGATGTTTGCCCGTAAGGTTGAAATTCGTAGGGCTGTTGAGGCAGAGGGAATCCCCCATACATTTGTTGTTAGCAATTTCTTCGATGGTTACTATCTTCGCAACTTTTCTCAGCCAGGAGCCAGCAACCCACCTAGAGACAAAATCAAAATCTTAGGGGATGGAAGTCTCAAAGGTATAATATGTTAGATTTCAATCTCATGATGTACAGCAATATAATCCCATTTTCTTGAGCTGAGATCTCCCCAGAAATTCAAAAGACATTGGTTTTCTTTTCTTTTAATAAACACACTTCTATATAGCATAATACCATATATATACACATGTTAATATACAATAGGCTGCAACAATTTCTTAAAAGGAAACAGGTTTGGTTTTAACAGCCTCTCGCACCATTCATTGTTCATCTGTGTTGTTTTTGTGAAGTGATTTACAACAAGGAAGAAGACATTGGAACATACACCATCCGAGCCATAGATGATCCAAGAACTCTCAACAAGATCTTGTACATTCGACCTCCTGCTAACATCTTCTCGACTAATGAGCTAGTCTCACTGTGGTAGAGAAAAATTGGCAGGACTCTTGAAAGGACCTACGTCCCAGAGGAGGAGCTTGTGAAGAACATCCAAGGTTTGTTTGGACAAAGCAACAATTTGTCGTTTAATCTACCCTGCTTCTTCATAAAAAGACCAAAACTCATTCGTATTTTCTTATCTTCTTTTGCAGAGACTCCATTTCCACTTAATACAGCATTAGCAATTAGTCACAGTGCTTTTGTGAAGGGAGATCATACCAACTTTGATATTGAACCATCACTTGGAGTGGAAGCTTCAGAACTGTATCCTGATGTTCAATACACAACTGTAGAAGATTACCTCAACCAGTTTGTTTAG

mRNA sequence

ATGCGTTTTGATCTTGATACAACAAAAACAGAGTGGAGAAGCATCATCTTCTTTGCATTTGCAGCTCACCGGTACAGCTCCGATCAAGCATTGTTCGCCGGAAACCATAATCCGATGACTCAGAAGCTCAAGGTTCTCATCATCGCAACTGGTTACATCGGAATTTTTTTTGTAGAAGCGAGCGTCAAAGCAGGTCACCGTCACAGCTCCGATCAGGTTTTGTTTGCCGGAAACCTTAATTTGATGGCTCAGAAGCTCAAAGTTCTCATCATCGGAGGTACTGGCTACATCGGAAAATTCCTAGTAGAAGCCAGCGCAAAAGCAGGTCATCCAACTTATATATTGGTCCGCGAATCTACCCTCGCTGCCTCCACTAAGTCTTCGATCATCGATAATTTCAGGAGCTTGGGTGTCAATTTCGTCTTCGGCGATATCTTCGACAATGAGAGTCTGGTTAGGGCAATACAGCAGGTTGATGTGGTGATATCCACCGTTGGTCGTTCTCTGTTATCCGATCAAGATAAGATCATATCTGCTATCAAACAAGCTGGTAATGTTAAGAGATTTTTTCCCTCTGAGTTTGGCAACGATGTGGATCGTGTTCATGCCGTGGAGCCAGCAAAATCGATGTTTGCCCGTAAGGTTGAAATTCGTAGGGCTGTTGAGGCAGAGGGAATCCCCCATACATTTGTTGTTAGCAATTTCTTCGATGGTTACTATCTTCGCAACTTTTCTCAGCCAGGAGCCAGCAACCCACCTAGAGACAAAATCAAAATCTTAGGGGATGGAAGTCTCAAAGTGATTTACAACAAGGAAGAAGACATTGGAACATACACCATCCGAGCCATAGATGATCCAAGAACTCTCAACAAGATCTTGACTCTTGAAAGGACCTACGTCCCAGAGGAGGAGCTTGTGAAGAACATCCAAGAGACTCCATTTCCACTTAATACAGCATTAGCAATTAGTCACAGTGCTTTTGTGAAGGGAGATCATACCAACTTTGATATTGAACCATCACTTGGAGTGGAAGCTTCAGAACTGTATCCTGATGTTCAATACACAACTGTAGAAGATTACCTCAACCAGTTTGTTTAG

Coding sequence (CDS)

ATGCGTTTTGATCTTGATACAACAAAAACAGAGTGGAGAAGCATCATCTTCTTTGCATTTGCAGCTCACCGGTACAGCTCCGATCAAGCATTGTTCGCCGGAAACCATAATCCGATGACTCAGAAGCTCAAGGTTCTCATCATCGCAACTGGTTACATCGGAATTTTTTTTGTAGAAGCGAGCGTCAAAGCAGGTCACCGTCACAGCTCCGATCAGGTTTTGTTTGCCGGAAACCTTAATTTGATGGCTCAGAAGCTCAAAGTTCTCATCATCGGAGGTACTGGCTACATCGGAAAATTCCTAGTAGAAGCCAGCGCAAAAGCAGGTCATCCAACTTATATATTGGTCCGCGAATCTACCCTCGCTGCCTCCACTAAGTCTTCGATCATCGATAATTTCAGGAGCTTGGGTGTCAATTTCGTCTTCGGCGATATCTTCGACAATGAGAGTCTGGTTAGGGCAATACAGCAGGTTGATGTGGTGATATCCACCGTTGGTCGTTCTCTGTTATCCGATCAAGATAAGATCATATCTGCTATCAAACAAGCTGGTAATGTTAAGAGATTTTTTCCCTCTGAGTTTGGCAACGATGTGGATCGTGTTCATGCCGTGGAGCCAGCAAAATCGATGTTTGCCCGTAAGGTTGAAATTCGTAGGGCTGTTGAGGCAGAGGGAATCCCCCATACATTTGTTGTTAGCAATTTCTTCGATGGTTACTATCTTCGCAACTTTTCTCAGCCAGGAGCCAGCAACCCACCTAGAGACAAAATCAAAATCTTAGGGGATGGAAGTCTCAAAGTGATTTACAACAAGGAAGAAGACATTGGAACATACACCATCCGAGCCATAGATGATCCAAGAACTCTCAACAAGATCTTGACTCTTGAAAGGACCTACGTCCCAGAGGAGGAGCTTGTGAAGAACATCCAAGAGACTCCATTTCCACTTAATACAGCATTAGCAATTAGTCACAGTGCTTTTGTGAAGGGAGATCATACCAACTTTGATATTGAACCATCACTTGGAGTGGAAGCTTCAGAACTGTATCCTGATGTTCAATACACAACTGTAGAAGATTACCTCAACCAGTTTGTTTAG

Protein sequence

MRFDLDTTKTEWRSIIFFAFAAHRYSSDQALFAGNHNPMTQKLKVLIIATGYIGIFFVEASVKAGHRHSSDQVLFAGNLNLMAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFVFGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRVHAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILGDGSLKVIYNKEEDIGTYTIRAIDDPRTLNKILTLERTYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTVEDYLNQFV
Homology
BLAST of HG10000464 vs. NCBI nr
Match: XP_008460946.1 (PREDICTED: isoflavone reductase homolog [Cucumis melo] >KAA0045561.1 isoflavone reductase-like protein [Cucumis melo var. makuwa] >TYK28832.1 isoflavone reductase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 501.1 bits (1289), Expect = 7.9e-138
Identity = 257/308 (83.44%), Postives = 270/308 (87.66%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           MAQKLKVLIIGGTGYIGKF+VEASA+AGHPT++LVR+STL+ S KSSIID+FR+LGVNFV
Sbjct: 1   MAQKLKVLIIGGTGYIGKFVVEASAQAGHPTFVLVRQSTLSDSAKSSIIDSFRNLGVNFV 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
           FGDIFDNESLVRAIQQVDVVISTVGR LLS Q  IISAIKQAGN+KRFFPSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQQNIISAIKQAGNIKRFFPSEFGNDVDRV 120

Query: 202 HAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILG 261
           HAVEPAKSMFARKVEIRR VEAEGIPHTFVVSNFFDGYYLRNFSQPGA+ PPRDKIKI G
Sbjct: 121 HAVEPAKSMFARKVEIRRVVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 180

Query: 262 DGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLER 321
           DG+LKVIYNKEEDIGTYTIRAIDDPRT NKIL                         LE+
Sbjct: 181 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKICRILEK 240

Query: 322 TYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTV 366
           TYV EEELVKNIQETP PLNTALAISHSAFVKGDHTNFDIEPS+GVEASELYPDV YTTV
Sbjct: 241 TYVSEEELVKNIQETPVPLNTALAISHSAFVKGDHTNFDIEPSIGVEASELYPDVHYTTV 300

BLAST of HG10000464 vs. NCBI nr
Match: XP_023006885.1 (isoflavone reductase-like protein [Cucurbita maxima])

HSP 1 Score: 493.0 bits (1268), Expect = 2.2e-135
Identity = 250/308 (81.17%), Postives = 271/308 (87.99%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           MAQKLKVLIIGGTGYIGKF+VEASAKAGHPTY+LVRESTLA S KSSIIDNFRSLGVNF+
Sbjct: 1   MAQKLKVLIIGGTGYIGKFIVEASAKAGHPTYVLVRESTLADSAKSSIIDNFRSLGVNFL 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
           FGDIFDNESLV+AI+QVDVVISTVGR+LL+++DKIISAIKQAGNVKRF PSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVKAIKQVDVVISTVGRALLANEDKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 202 HAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILG 261
           HAVEPAKSMFA K EIRRA+EAEGIPHTFVV NFFDGY+LRNF QPGA++PPRDKIKI+G
Sbjct: 121 HAVEPAKSMFAAKAEIRRAIEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 180

Query: 262 DGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLER 321
           DGS KVIYNKEEDIGTYTIRAI+DPRTLNKIL                        TLER
Sbjct: 181 DGSPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWEKKIGKTLER 240

Query: 322 TYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTV 366
            Y+PEEEL+KNIQE PFPLNT LAISH+AFVKGDHTNF+IEPS GVEASELY DV+YTTV
Sbjct: 241 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFEIEPSFGVEASELYSDVKYTTV 300

BLAST of HG10000464 vs. NCBI nr
Match: XP_022947777.1 (isoflavone reductase-like protein [Cucurbita moschata] >KAG6605074.1 Phenylcoumaran benzylic ether reductase Pyrc5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 493.0 bits (1268), Expect = 2.2e-135
Identity = 252/308 (81.82%), Postives = 270/308 (87.66%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           MAQKLKVLIIGGTGYIGKF+VEASAKAGHPTY LVRESTLA S KSSIIDNFRSLGVNF+
Sbjct: 1   MAQKLKVLIIGGTGYIGKFIVEASAKAGHPTYALVRESTLADSAKSSIIDNFRSLGVNFL 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
           FGDIFDNESLV+AI+QVDVVISTVGR+LL++QDKIISAIKQAGNVKRF PSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVKAIKQVDVVISTVGRALLANQDKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 202 HAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILG 261
           HAVEPAKSMFA K EIRRA+EAEGIPHTFVV NFFDGY+LRNF QPGA++PPRDKIKI+G
Sbjct: 121 HAVEPAKSMFAAKAEIRRAIEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 180

Query: 262 DGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLER 321
           DGS KVIYNKEEDIGTYTIRAI+DPRTLNKIL                        TLER
Sbjct: 181 DGSPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWEKKIGTTLER 240

Query: 322 TYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTV 366
            YVPEEEL+KNIQE PFPLNT LAISH+AFVKGDHTNF+IEPS GVEASELY DV+YTTV
Sbjct: 241 IYVPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFEIEPSFGVEASELYSDVKYTTV 300

BLAST of HG10000464 vs. NCBI nr
Match: KAG7035082.1 (hypothetical protein SDJN02_01877, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 491.1 bits (1263), Expect = 8.2e-135
Identity = 250/308 (81.17%), Postives = 270/308 (87.66%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           MAQKLKVLIIGGTGYIGKF+VEASAKAGHPTY LVRESTLA S KSSIIDNFRSLGVNF+
Sbjct: 1   MAQKLKVLIIGGTGYIGKFIVEASAKAGHPTYALVRESTLADSAKSSIIDNFRSLGVNFL 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
           FGDIFDNESLV+AI+QVDVVISTVGR+LL++QDKIISAIKQAGNVKRF PSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVKAIKQVDVVISTVGRALLANQDKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 202 HAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILG 261
           HAVEPAKSMFA K EIRRA+EAEGIPHTFV+ NFFDGY+LRNF QPGA++PPRDKIKI+G
Sbjct: 121 HAVEPAKSMFAAKAEIRRAIEAEGIPHTFVICNFFDGYFLRNFCQPGATDPPRDKIKIIG 180

Query: 262 DGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLER 321
           DGS KVIYNKEEDIGTYTIRAI+DPRTLNKIL                        TLE+
Sbjct: 181 DGSPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWEKKIGTTLEQ 240

Query: 322 TYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTV 366
            YVPEEEL+KNIQE PFPLNT LAISH+AFVKGDHTNF+IEPS GVEASELY DV+YTTV
Sbjct: 241 IYVPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFEIEPSFGVEASELYSDVKYTTV 300

BLAST of HG10000464 vs. NCBI nr
Match: XP_031736768.1 (phenylcoumaran benzylic ether reductase POP1 [Cucumis sativus] >KGN61804.1 hypothetical protein Csa_006062 [Cucumis sativus])

HSP 1 Score: 491.1 bits (1263), Expect = 8.2e-135
Identity = 254/308 (82.47%), Postives = 267/308 (86.69%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           MAQ LKVL+IG TGYIGKF+VEASA+AGHPT+ LVR+STLA S KSSII NFR+LGVNFV
Sbjct: 1   MAQNLKVLVIGATGYIGKFVVEASAQAGHPTFALVRQSTLADSAKSSIIHNFRNLGVNFV 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
           FGDIFDNESLVRAIQQVDVVISTVGR LLS Q+KIISAIKQAGNVKRF PSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQEKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 202 HAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILG 261
           HAVEPAKSMFA KVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGA+ PPRDKIKI G
Sbjct: 121 HAVEPAKSMFASKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 180

Query: 262 DGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLER 321
           DG+LKVIYNKEEDIGTYTIRAIDDPRT NKIL                         LER
Sbjct: 181 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKIGRILER 240

Query: 322 TYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTV 366
           TYV EEELVKNIQETP PL+TALAISHSAFVKGDHTNF+IEPS+GVEASELYP+V YTTV
Sbjct: 241 TYVSEEELVKNIQETPVPLSTALAISHSAFVKGDHTNFEIEPSIGVEASELYPNVHYTTV 300

BLAST of HG10000464 vs. ExPASy Swiss-Prot
Match: O81355 (Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC5 PE=1 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 5.2e-116
Identity = 208/308 (67.53%), Postives = 249/308 (80.84%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           MA K ++L IGGTGYIGKF+VEASAKAG+PTY+LVRE++L+   KS +I+NF++LGVNFV
Sbjct: 1   MASKSQILFIGGTGYIGKFIVEASAKAGYPTYVLVREASLSDPAKSKVIENFKALGVNFV 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
            GD++D+ESLV+AI+QVDVVISTVG   L+DQ KII+AIK+AGNVKRFFPSEFGNDVDR 
Sbjct: 61  LGDLYDHESLVKAIKQVDVVISTVGHGQLADQGKIIAAIKEAGNVKRFFPSEFGNDVDRS 120

Query: 202 HAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILG 261
           HAVEPAKS F  K +IRRAVEAEGIP+T+V SNFF GY+L   +QPGAS+ PRDK+ ILG
Sbjct: 121 HAVEPAKSAFETKAKIRRAVEAEGIPYTYVSSNFFAGYFLPTLNQPGASSAPRDKVVILG 180

Query: 262 DGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLER 321
           DG+ K I+NKE+DIGTYTIRA+DDPRTLNK+L                        TLER
Sbjct: 181 DGNPKAIFNKEDDIGTYTIRAVDDPRTLNKVLYIRPPANTISFNELVSLWEKKIGKTLER 240

Query: 322 TYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTV 366
            YVPEE+L+KNIQE   PLN  L+ISH+ FVKGDHTNF+IEPS GVEA+ LYPDV+YTTV
Sbjct: 241 IYVPEEQLLKNIQEAAVPLNVILSISHAVFVKGDHTNFEIEPSFGVEATALYPDVKYTTV 300

BLAST of HG10000464 vs. ExPASy Swiss-Prot
Match: B9HRL7 (Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=PCBER PE=1 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 2.0e-115
Identity = 205/303 (67.66%), Postives = 247/303 (81.52%), Query Frame = 0

Query: 87  KVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFVFGDIF 146
           K+L IGGTGYIGKF+VEASAKAGHPT++LVRESTL+   KS +I NF++LGVNF+ GD+F
Sbjct: 4   KILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSNPAKSVVIYNFKNLGVNFLIGDLF 63

Query: 147 DNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRVHAVEP 206
           D+ESLV+AI+QVDVVISTVG + L +QD+II+AIK+AGNVKRFFPSEFGNDVDRV+AVEP
Sbjct: 64  DHESLVKAIKQVDVVISTVGHAQLVEQDRIIAAIKEAGNVKRFFPSEFGNDVDRVNAVEP 123

Query: 207 AKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILGDGSLK 266
           AKS FA K  +RRA+EAEGIP+T+V SNFF GY+L +F+QPGA+ PPRDK+ ILGDG+ K
Sbjct: 124 AKSAFATKANVRRAIEAEGIPYTYVSSNFFSGYFLLSFNQPGATAPPRDKVVILGDGNPK 183

Query: 267 VIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLERTYVPE 326
            ++NKE+DI TYTI+A+DDPRTLNKIL                        TLER YVPE
Sbjct: 184 AVFNKEDDIATYTIKAVDDPRTLNKILYIKPPANTISFNDLVSLWEKKIGKTLERIYVPE 243

Query: 327 EELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTVEDYLN 366
           E+L+KNIQE   P+N  L+I HS FVKGDHTNF+IEPS GVEASELYPDV+YTTV++YL 
Sbjct: 244 EQLLKNIQEASVPVNVVLSIGHSVFVKGDHTNFEIEPSFGVEASELYPDVKYTTVDEYLK 303

BLAST of HG10000464 vs. ExPASy Swiss-Prot
Match: Q9FUW6 (Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6 PE=1 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.3e-111
Identity = 197/308 (63.96%), Postives = 243/308 (78.90%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           MA K K+LIIGGTGYIGKF+VEASAK+GHPT+ LVREST++   K  +++ F+ LGV  +
Sbjct: 1   MAHKSKILIIGGTGYIGKFIVEASAKSGHPTFALVRESTVSDPVKGKLVEKFKGLGVTLL 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
            GD++D+ESLV+A +QVDVVISTVG   L+DQ KII+AIK+AGN+KRFFPSEFGNDVDRV
Sbjct: 61  HGDLYDHESLVKAFKQVDVVISTVGHLQLADQVKIIAAIKEAGNIKRFFPSEFGNDVDRV 120

Query: 202 HAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILG 261
           HAVEPAK+ FA K EIRR  EAEGIP+T+V SNFF GY+L   +QPG ++PPR+K+ I G
Sbjct: 121 HAVEPAKTAFATKAEIRRKTEAEGIPYTYVSSNFFAGYFLPTLAQPGLTSPPREKVVIFG 180

Query: 262 DGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLER 321
           DG+ + ++NKE+DIGTYTIRA+DDPRTLNKI+                        TLE+
Sbjct: 181 DGNARAVFNKEDDIGTYTIRAVDDPRTLNKIVYIKPAKNIYSFNEIVALWEKKIGKTLEK 240

Query: 322 TYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTV 366
            YVPEE+L+K+IQE+P P+N  LAI+HS FVKGDHTNF+IE S GVEASELYPDV+YTTV
Sbjct: 241 IYVPEEKLLKDIQESPIPINVILAINHSVFVKGDHTNFEIEASFGVEASELYPDVKYTTV 300

BLAST of HG10000464 vs. ExPASy Swiss-Prot
Match: P52578 (Isoflavone reductase homolog OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 3.3e-110
Identity = 198/308 (64.29%), Postives = 241/308 (78.25%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           MA K K+L IGGTGYIGKF+VEASAKAGH T++LVRESTL+  TK+ +ID F+S GV FV
Sbjct: 1   MAGKSKILFIGGTGYIGKFIVEASAKAGHDTFVLVRESTLSNPTKTKLIDTFKSFGVTFV 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
            GD++D+ESLV+AI+QVDVVISTVG +LL+DQ K+I+AIK+AGNVKRFFPSEFGNDVDRV
Sbjct: 61  HGDLYDHESLVKAIKQVDVVISTVGHALLADQVKLIAAIKEAGNVKRFFPSEFGNDVDRV 120

Query: 202 HAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILG 261
           HAVEPAK+ F  K +IRR VEAEGIP T+V + FF GY L N +QPGA+ PP DK+ ILG
Sbjct: 121 HAVEPAKAAFNTKAQIRRVVEAEGIPFTYVATFFFAGYSLPNLAQPGAAGPPNDKVVILG 180

Query: 262 DGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLER 321
            G+ K ++NKEEDIGTYTI A+DDP+TLNKIL                         LER
Sbjct: 181 HGNTKAVFNKEEDIGTYTINAVDDPKTLNKILYIKPPHNIITLNELVSLWEKKTGKNLER 240

Query: 322 TYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTV 366
            YVPEE+++KNIQE   P+N  L+I H+AFVKGDHTNF+IEPS GVEASE+YPDV+YT +
Sbjct: 241 LYVPEEQVLKNIQEASVPMNVGLSIYHTAFVKGDHTNFEIEPSFGVEASEVYPDVKYTPI 300

BLAST of HG10000464 vs. ExPASy Swiss-Prot
Match: B2WSN0 (Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 1.8e-108
Identity = 196/309 (63.43%), Postives = 244/309 (78.96%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           M  K K+LIIGGTGYIGKF+VEAS K GHPT+ LVRE+T++   K  +++ F++LGV+ +
Sbjct: 1   MGSKSKILIIGGTGYIGKFIVEASVKEGHPTFALVRETTVSDPVKGKLVEKFQNLGVSLL 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
           +GD++D++SLV+AI+QVDVVISTVG   ++DQ KII+AIK+AGNVKRFFPSEFGNDVD V
Sbjct: 61  YGDLYDHDSLVKAIKQVDVVISTVGFMQIADQTKIIAAIKEAGNVKRFFPSEFGNDVDHV 120

Query: 202 HAVEPAKSM-FARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKIL 261
           +AVEPAKS+ FA K  IRRAVEAEGIP+T+V SN F+GY+L    QPGA+ PPRDK+ I 
Sbjct: 121 NAVEPAKSVAFAVKANIRRAVEAEGIPYTYVASNCFNGYFLPTLVQPGATTPPRDKVIIP 180

Query: 262 GDGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLE 321
           GDG+ K I+NKEEDIGTYTI+A+DDPRTLNKIL                        TLE
Sbjct: 181 GDGNPKAIFNKEEDIGTYTIKAVDDPRTLNKILYLRPSNNIYSFNELVALWEKKIGKTLE 240

Query: 322 RTYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTT 366
           + YVPEE+++K+IQE P P+N  L I+HS FVKGDHTNF+IEPS GVEASELYP+V+YTT
Sbjct: 241 KIYVPEEQILKDIQEAPIPINIFLGINHSVFVKGDHTNFEIEPSFGVEASELYPEVKYTT 300

BLAST of HG10000464 vs. ExPASy TrEMBL
Match: A0A5A7TVM0 (Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00380 PE=4 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 3.8e-138
Identity = 257/308 (83.44%), Postives = 270/308 (87.66%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           MAQKLKVLIIGGTGYIGKF+VEASA+AGHPT++LVR+STL+ S KSSIID+FR+LGVNFV
Sbjct: 1   MAQKLKVLIIGGTGYIGKFVVEASAQAGHPTFVLVRQSTLSDSAKSSIIDSFRNLGVNFV 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
           FGDIFDNESLVRAIQQVDVVISTVGR LLS Q  IISAIKQAGN+KRFFPSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQQNIISAIKQAGNIKRFFPSEFGNDVDRV 120

Query: 202 HAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILG 261
           HAVEPAKSMFARKVEIRR VEAEGIPHTFVVSNFFDGYYLRNFSQPGA+ PPRDKIKI G
Sbjct: 121 HAVEPAKSMFARKVEIRRVVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 180

Query: 262 DGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLER 321
           DG+LKVIYNKEEDIGTYTIRAIDDPRT NKIL                         LE+
Sbjct: 181 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKICRILEK 240

Query: 322 TYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTV 366
           TYV EEELVKNIQETP PLNTALAISHSAFVKGDHTNFDIEPS+GVEASELYPDV YTTV
Sbjct: 241 TYVSEEELVKNIQETPVPLNTALAISHSAFVKGDHTNFDIEPSIGVEASELYPDVHYTTV 300

BLAST of HG10000464 vs. ExPASy TrEMBL
Match: A0A1S3CET9 (isoflavone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103499681 PE=4 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 3.8e-138
Identity = 257/308 (83.44%), Postives = 270/308 (87.66%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           MAQKLKVLIIGGTGYIGKF+VEASA+AGHPT++LVR+STL+ S KSSIID+FR+LGVNFV
Sbjct: 1   MAQKLKVLIIGGTGYIGKFVVEASAQAGHPTFVLVRQSTLSDSAKSSIIDSFRNLGVNFV 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
           FGDIFDNESLVRAIQQVDVVISTVGR LLS Q  IISAIKQAGN+KRFFPSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQQNIISAIKQAGNIKRFFPSEFGNDVDRV 120

Query: 202 HAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILG 261
           HAVEPAKSMFARKVEIRR VEAEGIPHTFVVSNFFDGYYLRNFSQPGA+ PPRDKIKI G
Sbjct: 121 HAVEPAKSMFARKVEIRRVVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 180

Query: 262 DGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLER 321
           DG+LKVIYNKEEDIGTYTIRAIDDPRT NKIL                         LE+
Sbjct: 181 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKICRILEK 240

Query: 322 TYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTV 366
           TYV EEELVKNIQETP PLNTALAISHSAFVKGDHTNFDIEPS+GVEASELYPDV YTTV
Sbjct: 241 TYVSEEELVKNIQETPVPLNTALAISHSAFVKGDHTNFDIEPSIGVEASELYPDVHYTTV 300

BLAST of HG10000464 vs. ExPASy TrEMBL
Match: A0A6J1L1G1 (isoflavone reductase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111499544 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 1.0e-135
Identity = 250/308 (81.17%), Postives = 271/308 (87.99%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           MAQKLKVLIIGGTGYIGKF+VEASAKAGHPTY+LVRESTLA S KSSIIDNFRSLGVNF+
Sbjct: 1   MAQKLKVLIIGGTGYIGKFIVEASAKAGHPTYVLVRESTLADSAKSSIIDNFRSLGVNFL 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
           FGDIFDNESLV+AI+QVDVVISTVGR+LL+++DKIISAIKQAGNVKRF PSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVKAIKQVDVVISTVGRALLANEDKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 202 HAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILG 261
           HAVEPAKSMFA K EIRRA+EAEGIPHTFVV NFFDGY+LRNF QPGA++PPRDKIKI+G
Sbjct: 121 HAVEPAKSMFAAKAEIRRAIEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 180

Query: 262 DGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLER 321
           DGS KVIYNKEEDIGTYTIRAI+DPRTLNKIL                        TLER
Sbjct: 181 DGSPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWEKKIGKTLER 240

Query: 322 TYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTV 366
            Y+PEEEL+KNIQE PFPLNT LAISH+AFVKGDHTNF+IEPS GVEASELY DV+YTTV
Sbjct: 241 IYIPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFEIEPSFGVEASELYSDVKYTTV 300

BLAST of HG10000464 vs. ExPASy TrEMBL
Match: A0A6J1G7E0 (isoflavone reductase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111451536 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 1.0e-135
Identity = 252/308 (81.82%), Postives = 270/308 (87.66%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           MAQKLKVLIIGGTGYIGKF+VEASAKAGHPTY LVRESTLA S KSSIIDNFRSLGVNF+
Sbjct: 1   MAQKLKVLIIGGTGYIGKFIVEASAKAGHPTYALVRESTLADSAKSSIIDNFRSLGVNFL 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
           FGDIFDNESLV+AI+QVDVVISTVGR+LL++QDKIISAIKQAGNVKRF PSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVKAIKQVDVVISTVGRALLANQDKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 202 HAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILG 261
           HAVEPAKSMFA K EIRRA+EAEGIPHTFVV NFFDGY+LRNF QPGA++PPRDKIKI+G
Sbjct: 121 HAVEPAKSMFAAKAEIRRAIEAEGIPHTFVVCNFFDGYFLRNFCQPGATDPPRDKIKIIG 180

Query: 262 DGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLER 321
           DGS KVIYNKEEDIGTYTIRAI+DPRTLNKIL                        TLER
Sbjct: 181 DGSPKVIYNKEEDIGTYTIRAIEDPRTLNKILYIRPPANIYSTNELVSLWEKKIGTTLER 240

Query: 322 TYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTV 366
            YVPEEEL+KNIQE PFPLNT LAISH+AFVKGDHTNF+IEPS GVEASELY DV+YTTV
Sbjct: 241 IYVPEEELLKNIQEAPFPLNTVLAISHTAFVKGDHTNFEIEPSFGVEASELYSDVKYTTV 300

BLAST of HG10000464 vs. ExPASy TrEMBL
Match: A0A0A0LL65 (NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G248135 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 4.0e-135
Identity = 254/308 (82.47%), Postives = 267/308 (86.69%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           MAQ LKVL+IG TGYIGKF+VEASA+AGHPT+ LVR+STLA S KSSII NFR+LGVNFV
Sbjct: 1   MAQNLKVLVIGATGYIGKFVVEASAQAGHPTFALVRQSTLADSAKSSIIHNFRNLGVNFV 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
           FGDIFDNESLVRAIQQVDVVISTVGR LLS Q+KIISAIKQAGNVKRF PSEFGNDVDRV
Sbjct: 61  FGDIFDNESLVRAIQQVDVVISTVGRGLLSHQEKIISAIKQAGNVKRFLPSEFGNDVDRV 120

Query: 202 HAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILG 261
           HAVEPAKSMFA KVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGA+ PPRDKIKI G
Sbjct: 121 HAVEPAKSMFASKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGATEPPRDKIKIFG 180

Query: 262 DGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLER 321
           DG+LKVIYNKEEDIGTYTIRAIDDPRT NKIL                         LER
Sbjct: 181 DGNLKVIYNKEEDIGTYTIRAIDDPRTFNKILYIRPPANIYSTNELVSLWEKKIGRILER 240

Query: 322 TYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTV 366
           TYV EEELVKNIQETP PL+TALAISHSAFVKGDHTNF+IEPS+GVEASELYP+V YTTV
Sbjct: 241 TYVSEEELVKNIQETPVPLSTALAISHSAFVKGDHTNFEIEPSIGVEASELYPNVHYTTV 300

BLAST of HG10000464 vs. TAIR 10
Match: AT4G39230.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 381.7 bits (979), Expect = 6.5e-106
Identity = 191/308 (62.01%), Postives = 235/308 (76.30%), Query Frame = 0

Query: 82  MAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFV 141
           M  K K+L IGGTGYIGK++VEASA++GHPT +LVR STL + ++SS I+NF++LGV F+
Sbjct: 1   MTSKSKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFL 60

Query: 142 FGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRV 201
            GD+ D+ SLV +I+Q DVVISTVG SLL  Q KIISAIK+AGNVKRFFPSEFGNDVDRV
Sbjct: 61  LGDLDDHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRV 120

Query: 202 HAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQPGASNPPRDKIKILG 261
             VEPAKS +A K +IRR +EAEGIP+T+V  NFF GY+L   +QPGA++ PRDK+ +LG
Sbjct: 121 FTVEPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLG 180

Query: 262 DGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLER 321
           DG+ K ++NKEEDIGTYTI A+DDPRTLNKIL                        TLER
Sbjct: 181 DGNPKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLER 240

Query: 322 TYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTV 366
            YVPEE+L+K I E+  PLN  L++ H  FVKG HT+F+IEPS GVEASELYPDV+YTTV
Sbjct: 241 IYVPEEQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTV 300

BLAST of HG10000464 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 359.8 bits (922), Expect = 2.7e-99
Identity = 183/310 (59.03%), Postives = 230/310 (74.19%), Query Frame = 0

Query: 81  LMAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNF 140
           +  +K K+L+IGGTGYIGKFLVEASAKAGH T+ LVRE+TL+   K   + +F+ LGV  
Sbjct: 1   MATEKSKILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTI 60

Query: 141 VFGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDR 200
           + GD+ D+ESLV+AI+QVDVVISTVG   + DQ KIISAIK+AGNVKRF PSEFG DVDR
Sbjct: 61  LHGDLNDHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDR 120

Query: 201 VHAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQ--PGASNPPRDKIK 260
             AVEPAKS FA K++IRR +EAEGIP+T+ V+  F GYYL    Q  PG ++PPRDK+ 
Sbjct: 121 TSAVEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVT 180

Query: 261 ILGDGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------T 320
           ILGDG+ K + NKEEDI  YTI+A+DDPRTLNKIL                        +
Sbjct: 181 ILGDGNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKS 240

Query: 321 LERTYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQY 365
           LE+T++PEE+L+K+IQE+P P+N  L+I+H+ FV GD TN  IEPS GVEASELYPDV+Y
Sbjct: 241 LEKTHLPEEQLLKSIQESPIPINVVLSINHAVFVNGD-TNISIEPSFGVEASELYPDVKY 300

BLAST of HG10000464 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 347.1 bits (889), Expect = 1.8e-95
Identity = 170/310 (54.84%), Postives = 225/310 (72.58%), Query Frame = 0

Query: 81  LMAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNF 140
           + ++K K+L+IGGTG+IGK ++EAS KAGH T  LVRE++L+   K   + NF+  GV  
Sbjct: 1   MASEKSKILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTL 60

Query: 141 VFGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDR 200
           + GD+ D+ESLV+AI+Q DVVISTVG   + DQ KIISAIK+AGNVKRF PSEFG DVD+
Sbjct: 61  LHGDLNDHESLVKAIKQADVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDK 120

Query: 201 VHAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQ--PGASNPPRDKIK 260
             AVEPAKS F RK++ RR +EAEGIP+T++V+N+F GYYL    Q  PG ++PPRDK+K
Sbjct: 121 SSAVEPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVK 180

Query: 261 ILGDGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------T 320
           I GDG++K + NKEEDI  YTI+A+DDPRTLNK L                        +
Sbjct: 181 IFGDGNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKS 240

Query: 321 LERTYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQY 365
           +E+ Y+ EE++ K+IQE+P P N  L+I+H+ FVKGD TNF IEPS G EASELYPD++Y
Sbjct: 241 VEKIYMSEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDIKY 300

BLAST of HG10000464 vs. TAIR 10
Match: AT1G75300.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 321.6 bits (823), Expect = 8.0e-88
Identity = 170/324 (52.47%), Postives = 226/324 (69.75%), Query Frame = 0

Query: 81  LMAQKLKVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNF 140
           +  +K K+L+IGGTGY+G+F+VE SAKAG+PT+ LVRE++L+   KS  I +F+ LGV  
Sbjct: 1   MTTEKSKILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTI 60

Query: 141 VFGDIFDNESLVRAIQQVDVVISTVGRSLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDR 200
           + GD+ D+ESLV+AI+QVDVVIST+G   + DQ KIISAIK+AGNVKRF P+EFG DV+R
Sbjct: 61  LHGDLNDHESLVKAIKQVDVVISTIGHKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVER 120

Query: 201 VHAVEPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQ-------------- 260
             AVEPAKS+FA KV+IRRA+EAEGIP+T+VVSN   G+YLR   Q              
Sbjct: 121 TSAVEPAKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAI 180

Query: 261 -PGASN-PPRDKIKILGDGSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL----------- 320
             G  N PPRDK+ ILGDG+ KV+ NKEED+  Y I+A+DD RTLNK L           
Sbjct: 181 IFGDKNVPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSM 240

Query: 321 -------------TLERTYVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPS 365
                        +LE+T++ EE+++K+IQ    P++   +I+H+ FVKGD T+F IEP 
Sbjct: 241 NEMVTLWEKKIGKSLEKTHISEEQILKSIQ---VPIDVFKSINHAVFVKGDQTSFTIEPW 300

BLAST of HG10000464 vs. TAIR 10
Match: AT1G19540.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 294.7 bits (753), Expect = 1.0e-79
Identity = 156/307 (50.81%), Postives = 209/307 (68.08%), Query Frame = 0

Query: 87  KVLIIGGTGYIGKFLVEASAKAGHPTYILVRESTLAASTKSSIIDNFRSLGVNFVFGDIF 146
           K+L+IG TG IGK LVE SAK+GH T+ LVRE++L+   K+ +++ F+ LGV  ++G + 
Sbjct: 4   KILVIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYGSLS 63

Query: 147 DNESLVRAIQQVDVVISTVGR--SLLSDQDKIISAIKQAGNVKRFFPSEFGNDVDRVHAV 206
           D ESLV+AI+QVDVVIS VGR  + + +Q  II AIK++GNVKRF PSEFGNDVDR  A+
Sbjct: 64  DKESLVKAIKQVDVVISAVGRFQTEILNQTNIIDAIKESGNVKRFLPSEFGNDVDRTVAI 123

Query: 207 EPAKSMFARKVEIRRAVEAEGIPHTFVVSNFFDGYYLRNFSQ--PGASNPPRDKIKILGD 266
           EP  S F  K +IRRA+EA  IP+T+VVS  F G ++    Q      +PPRDK+ I   
Sbjct: 124 EPTLSEFITKAQIRRAIEAAKIPYTYVVSGCFAGLFVPCLGQCHLRLRSPPRDKVSIYDT 183

Query: 267 GSLKVIYNKEEDIGTYTIRAIDDPRTLNKIL------------------------TLERT 326
           G+ K I N EEDI  YT++A+DDPRTLNKIL                        TLE+T
Sbjct: 184 GNGKAIVNTEEDIVAYTLKAVDDPRTLNKILYIHPPNYIVSQNDMVGLWEEKIGKTLEKT 243

Query: 327 YVPEEELVKNIQETPFPLNTALAISHSAFVKGDHTNFDIEPSLGVEASELYPDVQYTTVE 366
           YV EEEL+K IQE+  P++  + + H+  VK D T+F I+PS GVEASELYP+V+YT+V+
Sbjct: 244 YVSEEELLKTIQESKPPMDFLVGLIHTILVKSDFTSFTIDPSFGVEASELYPEVKYTSVD 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008460946.17.9e-13883.44PREDICTED: isoflavone reductase homolog [Cucumis melo] >KAA0045561.1 isoflavone ... [more]
XP_023006885.12.2e-13581.17isoflavone reductase-like protein [Cucurbita maxima][more]
XP_022947777.12.2e-13581.82isoflavone reductase-like protein [Cucurbita moschata] >KAG6605074.1 Phenylcouma... [more]
KAG7035082.18.2e-13581.17hypothetical protein SDJN02_01877, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_031736768.18.2e-13582.47phenylcoumaran benzylic ether reductase POP1 [Cucumis sativus] >KGN61804.1 hypot... [more]
Match NameE-valueIdentityDescription
O813555.2e-11667.53Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC... [more]
B9HRL72.0e-11567.66Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=P... [more]
Q9FUW62.3e-11163.96Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6... [more]
P525783.3e-11064.29Isoflavone reductase homolog OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
B2WSN01.8e-10863.43Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TVM03.8e-13883.44Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3CET93.8e-13883.44isoflavone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103499681 PE=4 SV=1[more]
A0A6J1L1G11.0e-13581.17isoflavone reductase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111499544 PE... [more]
A0A6J1G7E01.0e-13581.82isoflavone reductase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111451536 ... [more]
A0A0A0LL654.0e-13582.47NmrA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G248135 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT4G39230.16.5e-10662.01NmrA-like negative transcriptional regulator family protein [more]
AT1G75280.12.7e-9959.03NmrA-like negative transcriptional regulator family protein [more]
AT1G75290.11.8e-9554.84NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G75300.18.0e-8852.47NmrA-like negative transcriptional regulator family protein [more]
AT1G19540.11.0e-7950.81NmrA-like negative transcriptional regulator family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008030NmrA-like domainPFAMPF05368NmrAcoord: 88..296
e-value: 3.5E-66
score: 223.1
NoneNo IPR availableGENE3D3.40.50.720coord: 337..350
e-value: 2.7E-21
score: 77.9
NoneNo IPR availableGENE3D3.40.50.720coord: 87..294
e-value: 2.0E-89
score: 301.6
NoneNo IPR availableGENE3D3.90.25.10coord: 295..361
e-value: 2.7E-21
score: 77.9
NoneNo IPR availableGENE3D3.90.25.10coord: 234..272
e-value: 2.0E-89
score: 301.6
NoneNo IPR availablePANTHERPTHR43349PINORESINOL REDUCTASE-RELATEDcoord: 294..365
coord: 84..293
NoneNo IPR availablePANTHERPTHR43349:SF76ISOFLAVONE REDUCTASE HOMOLOG P3-RELATEDcoord: 294..365
coord: 84..293
NoneNo IPR availableCDDcd05259PCBER_SDR_acoord: 87..360
e-value: 2.70374E-70
score: 219.482
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 87..334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10000464.1HG10000464.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009807 lignan biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016491 oxidoreductase activity