HG10000427 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10000427 vs. NCBI nr
Match: XP_008459259.1 (PREDICTED: UPF0496 protein At4g34320-like [Cucumis melo] >KAA0045956.1 UPF0496 protein [Cucumis melo var. makuwa] >TYK13629.1 UPF0496 protein [Cucumis melo var. makuwa])

HSP 1 Score: 625.2 bits (1611), Expect = 3.7e-175
Identity = 333/368 (90.49%), Postives = 349/368 (94.84%), Query Frame = 0

Query: 1   MGNNHGTKRGSTTVATINLDAYEAACRADDDVRSFDKALQARANQVLTTLA-DGGVEVRA 60
           MGN H TKR    +    + AYEAACR DDDVRSFDK LQARANQVLTTLA DGGVEVRA
Sbjct: 1   MGNQHTTKRTPEELQPA-VAAYEAACRVDDDVRSFDKTLQARANQVLTTLADDGGVEVRA 60

Query: 61  LSLDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN 120
           LS DSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN
Sbjct: 61  LSFDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN 120

Query: 121 CLKRAQDSQVLILMAVRQFEEEERETQVGPNQFAKTLQELRNFKASGDPFTHDFFKIFHS 180
           CLKRA+DSQV+ILMAVRQFEE+ERE+Q+GPNQF KTL+ELRNFKASGDPFT +FF+IFHS
Sbjct: 121 CLKRARDSQVMILMAVRQFEEDERESQMGPNQFDKTLRELRNFKASGDPFTDEFFRIFHS 180

Query: 181 VYKHQTAMLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAPPVA 240
           VYKHQTAMLEKLQQKKNKLDKKLKSI+TWRKLSCMIFAATFAAVLICSVVAT+I APPVA
Sbjct: 181 VYKHQTAMLEKLQQKKNKLDKKLKSISTWRKLSCMIFAATFAAVLICSVVATVIMAPPVA 240

Query: 241 AALSAASSIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLVEKL 300
           AALSAASSIP+GSMGKWIDSLWKSYENA+KGQKEVINSMQVGTYIAIKDMDNIR+LVEKL
Sbjct: 241 AALSAASSIPLGSMGKWIDSLWKSYENAVKGQKEVINSMQVGTYIAIKDMDNIRILVEKL 300

Query: 301 EIEIEGMLEKADFAIKEEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVL 360
           EIEI+GMLEKADFAIKEEALKV VEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVL
Sbjct: 301 EIEIKGMLEKADFAIKEEALKVVVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVL 360

Query: 361 QRIIKHPN 368
           QRIIKHPN
Sbjct: 361 QRIIKHPN 367

BLAST of HG10000427 vs. NCBI nr
Match: XP_004145340.3 (UPF0496 protein At4g34320 [Cucumis sativus] >KAE8651866.1 hypothetical protein Csa_006272 [Cucumis sativus])

HSP 1 Score: 621.7 bits (1602), Expect = 4.0e-174
Identity = 331/367 (90.19%), Postives = 350/367 (95.37%), Query Frame = 0

Query: 1   MGNNHGTKRGSTTVATINLDAYEAACRADDDVRSFDKALQARANQVLTTLA-DGGVEVRA 60
           MGN+H TKR +T      + AYEAACRADDDVRSFDK LQARANQVLTTLA DGGVEVRA
Sbjct: 56  MGNHHTTKR-TTEELQPAVAAYEAACRADDDVRSFDKTLQARANQVLTTLADDGGVEVRA 115

Query: 61  LSLDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN 120
           LS DSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN
Sbjct: 116 LSFDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN 175

Query: 121 CLKRAQDSQVLILMAVRQFEEEERETQVGPNQFAKTLQELRNFKASGDPFTHDFFKIFHS 180
           CLKRA+DSQV+ILMAVRQFEE+ERE+Q+GPNQF KTL+ELRNFKASGDPFT +FF+IFHS
Sbjct: 176 CLKRARDSQVMILMAVRQFEEDERESQMGPNQFDKTLRELRNFKASGDPFTDEFFRIFHS 235

Query: 181 VYKHQTAMLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAPPVA 240
           VYKHQTAMLEKLQQKKNKLDKKLKSI+TWRKLSCM+FAATFAAVLICSVVAT+I APPVA
Sbjct: 236 VYKHQTAMLEKLQQKKNKLDKKLKSISTWRKLSCMMFAATFAAVLICSVVATVIMAPPVA 295

Query: 241 AALSAASSIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLVEKL 300
           AALSAASSIP+GSMGKWIDSLWKSYENA+KGQKEVINSMQVGTYIAIKDMDNIR+LVEKL
Sbjct: 296 AALSAASSIPLGSMGKWIDSLWKSYENAVKGQKEVINSMQVGTYIAIKDMDNIRILVEKL 355

Query: 301 EIEIEGMLEKADFAIKEEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVL 360
           EIEIEGMLEKADFAIKE+ALK  VEEMKKKLEVFMKSVEDLGVQADLCSRDITRART+VL
Sbjct: 356 EIEIEGMLEKADFAIKEDALKFVVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTLVL 415

Query: 361 QRIIKHP 367
           QRIIKHP
Sbjct: 416 QRIIKHP 421

BLAST of HG10000427 vs. NCBI nr
Match: XP_023007494.1 (UPF0496 protein At4g34320-like [Cucurbita maxima])

HSP 1 Score: 594.3 bits (1531), Expect = 6.9e-166
Identity = 320/375 (85.33%), Postives = 342/375 (91.20%), Query Frame = 0

Query: 1   MGNNHGTKRGST-------TVATINLDAYEAACRADDDVRSFDKALQARANQVLTTLA-D 60
           MGN+H TKR +         +A  +L  YEAAC AD DVRSFDKALQARANQVLTTLA D
Sbjct: 1   MGNSHATKRSTAAEQPVAINLAAKDLTTYEAACVADADVRSFDKALQARANQVLTTLADD 60

Query: 61  GGVEVRALSLDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLD 120
           GGV+VRALS DSLKQVTECLLEMNQEVVRVILQCKKDIWKNQE+FELVEDYFENSL+TLD
Sbjct: 61  GGVQVRALSFDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQEVFELVEDYFENSLQTLD 120

Query: 121 FCTALENCLKRAQDSQVLILMAVRQFEEEERETQVGPNQFAKTLQELRNFKASGDPFTHD 180
           FCTALENCLKRA+DSQ+LILMA+RQFEEE  ET VG N+F KTLQELRNFKAS DPFTH+
Sbjct: 121 FCTALENCLKRARDSQLLILMALRQFEEE--ETHVGRNRFVKTLQELRNFKASADPFTHE 180

Query: 181 FFKIFHSVYKHQTAMLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATI 240
           FF IFHSVYK QT MLEKLQQKKNKLDKKLKSINTWRKLS MIFAATFAAVLICSVVAT+
Sbjct: 181 FFNIFHSVYKQQTVMLEKLQQKKNKLDKKLKSINTWRKLSSMIFAATFAAVLICSVVATV 240

Query: 241 ITAPPVAAALSAASSIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNI 300
           I APPV AALSAASSIP+GSMGKWIDSLW+SYENALKGQKEVI+SMQVGTYIAIKDMDNI
Sbjct: 241 IAAPPVTAALSAASSIPLGSMGKWIDSLWRSYENALKGQKEVISSMQVGTYIAIKDMDNI 300

Query: 301 RVLVEKLEIEIEGMLEKADFAIKEEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDIT 360
           RVLV KLE E+EGMLEKA+FAI+EEA+KVGVEEMKKKL+VFMKSVEDLGVQADLCS+DIT
Sbjct: 301 RVLVGKLETEMEGMLEKAEFAIEEEAVKVGVEEMKKKLDVFMKSVEDLGVQADLCSKDIT 360

Query: 361 RARTVVLQRIIKHPN 368
           RARTVVLQRIIKHPN
Sbjct: 361 RARTVVLQRIIKHPN 373

BLAST of HG10000427 vs. NCBI nr
Match: KAG6605105.1 (UPF0496 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 589.0 bits (1517), Expect = 2.9e-164
Identity = 321/380 (84.47%), Postives = 343/380 (90.26%), Query Frame = 0

Query: 1   MGNNHGTKRGSTTV----ATINLDA-----YEAACRADDDVRSFDKALQARANQVLTTLA 60
           MGN+H TKR +TT     A INL A     YEAAC AD DVRSFDKALQARANQVLTTLA
Sbjct: 36  MGNSHATKRSTTTAAEQPAAINLAAKDLTTYEAACVADADVRSFDKALQARANQVLTTLA 95

Query: 61  D----GGVEVRALSLDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENS 120
           D    GGV+VRALS DSLKQVTECLLEMNQEVVRVILQCKKDIWK+QE+FELVEDYFENS
Sbjct: 96  DDGGGGGVQVRALSFDSLKQVTECLLEMNQEVVRVILQCKKDIWKSQEVFELVEDYFENS 155

Query: 121 LETLDFCTALENCLKRAQDSQVLILMAVRQFEEEERETQVGPNQFAKTLQELRNFKASGD 180
           L+TLDFCTALENCLKRA+DSQ+LILMA+RQFEEE  ET +G N+F KTLQELRNFKAS D
Sbjct: 156 LQTLDFCTALENCLKRARDSQLLILMALRQFEEE--ETHLGRNRFVKTLQELRNFKASAD 215

Query: 181 PFTHDFFKIFHSVYKHQTAMLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICS 240
           PFT +FF IFHSVYK Q  MLEKLQQKKNKLDKKLKSINTWRKLS MIFAATFAAVLICS
Sbjct: 216 PFTQEFFNIFHSVYKQQMVMLEKLQQKKNKLDKKLKSINTWRKLSSMIFAATFAAVLICS 275

Query: 241 VVATIITAPPVAAALSAASSIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIK 300
           VVAT+I APPV AALSAASSIP+GSMGKWIDSLW+SYENALKGQKEVI+SMQVGTYIAIK
Sbjct: 276 VVATVIAAPPVTAALSAASSIPLGSMGKWIDSLWRSYENALKGQKEVISSMQVGTYIAIK 335

Query: 301 DMDNIRVLVEKLEIEIEGMLEKADFAIKEEALKVGVEEMKKKLEVFMKSVEDLGVQADLC 360
           DMDNIRVLV KLE E+EGMLEKA+FAI+EEA+KVGVEEMKKKL+VFMKSVEDLGVQADLC
Sbjct: 336 DMDNIRVLVGKLETEMEGMLEKAEFAIEEEAVKVGVEEMKKKLDVFMKSVEDLGVQADLC 395

Query: 361 SRDITRARTVVLQRIIKHPN 368
           S+DITRARTVVLQRIIKHPN
Sbjct: 396 SKDITRARTVVLQRIIKHPN 413

BLAST of HG10000427 vs. NCBI nr
Match: XP_023533322.1 (UPF0496 protein At4g34320-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 588.6 bits (1516), Expect = 3.8e-164
Identity = 321/380 (84.47%), Postives = 343/380 (90.26%), Query Frame = 0

Query: 1   MGNNHGTKRGSTTV----ATINLDA-----YEAACRADDDVRSFDKALQARANQVLTTLA 60
           MGN+H TKR +T V    A INL A     YEAAC AD DVRSFDKALQARANQVLTTLA
Sbjct: 1   MGNSHATKRSTTAVEEQPAAINLAAKDLTTYEAACVADADVRSFDKALQARANQVLTTLA 60

Query: 61  D----GGVEVRALSLDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENS 120
           D    GGV+VRALS DSLKQVTECLLEMNQEVVRVILQCKKDIWK+QE+FELVEDYFENS
Sbjct: 61  DDGGGGGVQVRALSFDSLKQVTECLLEMNQEVVRVILQCKKDIWKSQEVFELVEDYFENS 120

Query: 121 LETLDFCTALENCLKRAQDSQVLILMAVRQFEEEERETQVGPNQFAKTLQELRNFKASGD 180
           L+TLDFCTALENCLKRA+DSQ+LILMA+RQFEEE  ET VG N+F KTLQELRNFKAS D
Sbjct: 121 LQTLDFCTALENCLKRARDSQLLILMALRQFEEE--ETHVGRNRFVKTLQELRNFKASAD 180

Query: 181 PFTHDFFKIFHSVYKHQTAMLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICS 240
           PFT +FF IFHSVYK Q  MLEKLQQKKNKLDKKLKSINTWRKLS MIFAATFAAVLICS
Sbjct: 181 PFTQEFFNIFHSVYKQQMVMLEKLQQKKNKLDKKLKSINTWRKLSSMIFAATFAAVLICS 240

Query: 241 VVATIITAPPVAAALSAASSIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIK 300
           VVAT+I APPV AALSAASSIP+GSMGKWIDSLW+SYENALKGQKEVI+SMQVGTYIAIK
Sbjct: 241 VVATVIAAPPVTAALSAASSIPLGSMGKWIDSLWRSYENALKGQKEVISSMQVGTYIAIK 300

Query: 301 DMDNIRVLVEKLEIEIEGMLEKADFAIKEEALKVGVEEMKKKLEVFMKSVEDLGVQADLC 360
           DMDNIRVLV K+E E+EGMLEKA+FAI+EEA+KVGVEEMKKKL+VFMKSVEDLGVQADLC
Sbjct: 301 DMDNIRVLVGKVETEMEGMLEKAEFAIEEEAVKVGVEEMKKKLDVFMKSVEDLGVQADLC 360

Query: 361 SRDITRARTVVLQRIIKHPN 368
           S+DITRARTVVLQRIIKHPN
Sbjct: 361 SKDITRARTVVLQRIIKHPN 378

BLAST of HG10000427 vs. ExPASy Swiss-Prot
Match: Q9SYZ7 (UPF0496 protein At4g34320 OS=Arabidopsis thaliana OX=3702 GN=At4g34320 PE=2 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 1.2e-133
Identity = 249/371 (67.12%), Postives = 309/371 (83.29%), Query Frame = 0

Query: 1   MGNNHGTKRGSTTVATIN----LDAYEAACRADDDVRSFDKALQARANQVLTTLADGGVE 60
           MGN    K   T+  +++    L +Y AAC+AD +++SFD  LQAR + V++TLA  GVE
Sbjct: 1   MGNQTSKKSQETSAKSVHYTTELRSYAAACKADTELQSFDTCLQARTSHVISTLAT-GVE 60

Query: 61  VRALSLDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTA 120
           VRALS DSLK+VT+CLLEMNQEVV+VIL CKKDIWKNQE+FELVEDYFENSL+TLDFC A
Sbjct: 61  VRALSFDSLKEVTQCLLEMNQEVVKVILDCKKDIWKNQEMFELVEDYFENSLKTLDFCAA 120

Query: 121 LENCLKRAQDSQVLILMAVRQFEEEERETQVGPNQFAKTLQELRNFKASGDPFTHDFFKI 180
           LE  L+RA+DS +LIL+A++QFE+E      G N + KTL+EL+NFK +  PF  DFFK+
Sbjct: 121 LEKGLRRARDSHLLILVALQQFEDE--SLVQGGNGYKKTLEELKNFKDAESPFNEDFFKM 180

Query: 181 FHSVYKHQTAMLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAP 240
           F SVYK Q  MLEKLQ +KNKLDKKLK I+TWRKLS +IF ATFA VLICSVVA  + AP
Sbjct: 181 FQSVYKQQMLMLEKLQHRKNKLDKKLKCIHTWRKLSSIIFVATFATVLICSVVAAAMAAP 240

Query: 241 PVAAALSAASSIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLV 300
           PVAAAL+AA+++P+GSMGKWIDSLWK+YENALKGQKEVI+SMQ GT++A+KD+DNIRVL+
Sbjct: 241 PVAAALAAATAVPLGSMGKWIDSLWKNYENALKGQKEVISSMQAGTFVAVKDLDNIRVLI 300

Query: 301 EKLEIEIEGMLEKADFAIKEEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRART 360
           E+LEIEI GM++ A+FA++  A+K+G++++KKKLEVF K+VE+LG QADLCSRDI RART
Sbjct: 301 ERLEIEITGMVKSAEFAVEHNAVKIGIDDIKKKLEVFKKNVEELGTQADLCSRDIRRART 360

Query: 361 VVLQRIIKHPN 368
           V+LQRIIKHPN
Sbjct: 361 VILQRIIKHPN 368

BLAST of HG10000427 vs. ExPASy Swiss-Prot
Match: A2XDK8 (UPF0496 protein 1 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_010151 PE=2 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.0e-123
Identity = 234/362 (64.64%), Postives = 301/362 (83.15%), Query Frame = 0

Query: 15  ATINLDAYEAACRADDDVRSFDKALQARANQVLTTLADGGVEVRALSLDSLKQVTECLLE 74
           A   L +YEAACR+D ++R+FD  LQ R ++ ++TLA  GVEVR+LSL+SL++VT CLL+
Sbjct: 28  AAEELSSYEAACRSDPELRTFDTTLQRRTSRAISTLA-VGVEVRSLSLESLREVTGCLLD 87

Query: 75  MNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALENCLKRAQDSQVLILMA 134
           MNQEVVRVIL CKKDIWK+ ELF+LVEDYFE+SL TLDFCTAL+ CLKRA+DSQ+L+ +A
Sbjct: 88  MNQEVVRVILDCKKDIWKSPELFDLVEDYFESSLHTLDFCTALDKCLKRARDSQLLLHVA 147

Query: 135 VRQFEEEE--------RETQVGPNQFAKTLQELRNFKASGDPFTHDFFKIFHSVYKHQTA 194
           +++F++EE        +E      ++A+TL ELR FKA+GDPFT +FF  F +VY+ Q  
Sbjct: 148 LQRFDDEEDNDAAAAGQEDAAPSARYARTLHELRQFKAAGDPFTEEFFSAFQAVYRQQLT 207

Query: 195 MLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAPPVAAALSAAS 254
           MLEKLQQ+K++LDKK+++I  WR++S +IFA TFAAVLICSVVA  I APPVAAAL+AA+
Sbjct: 208 MLEKLQQRKHRLDKKVRAIKAWRRVSSIIFATTFAAVLICSVVAAAIAAPPVAAALAAAA 267

Query: 255 SIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLVEKLEIEIEGM 314
           SIPVGSMGKWIDSL K Y++AL+GQKEV+++MQVGT+IAIKD+D+IRVL+ ++E+EI  M
Sbjct: 268 SIPVGSMGKWIDSLLKGYQDALRGQKEVVSAMQVGTFIAIKDLDSIRVLINRVELEISSM 327

Query: 315 LEKADFAIK-EEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVLQRIIKH 368
           ++  +FA + EEA+K GVEE+KKKLEVFMKSVEDLG QAD CSRDI RARTVVLQRII+H
Sbjct: 328 IDCVEFAERDEEAVKFGVEEIKKKLEVFMKSVEDLGEQADRCSRDIRRARTVVLQRIIRH 387

BLAST of HG10000427 vs. ExPASy Swiss-Prot
Match: Q10QE9 (UPF0496 protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0199100 PE=2 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.0e-123
Identity = 234/362 (64.64%), Postives = 301/362 (83.15%), Query Frame = 0

Query: 15  ATINLDAYEAACRADDDVRSFDKALQARANQVLTTLADGGVEVRALSLDSLKQVTECLLE 74
           A   L +YEAACR+D ++R+FD  LQ R ++ ++TLA  GVEVR+LSL+SL++VT CLL+
Sbjct: 28  AAEELSSYEAACRSDPELRTFDTTLQRRTSRAISTLA-VGVEVRSLSLESLREVTGCLLD 87

Query: 75  MNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALENCLKRAQDSQVLILMA 134
           MNQEVVRVIL CKKDIWK+ ELF+LVEDYFE+SL TLDFCTAL+ CLKRA+DSQ+L+ +A
Sbjct: 88  MNQEVVRVILDCKKDIWKSPELFDLVEDYFESSLHTLDFCTALDKCLKRARDSQLLLHVA 147

Query: 135 VRQFEEEE--------RETQVGPNQFAKTLQELRNFKASGDPFTHDFFKIFHSVYKHQTA 194
           +++F++EE        +E      ++A+TL ELR FKA+GDPFT +FF  F +VY+ Q  
Sbjct: 148 LQRFDDEEDNDAAAAGQEDAAPSARYARTLHELRQFKAAGDPFTEEFFSAFQAVYRQQLT 207

Query: 195 MLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAPPVAAALSAAS 254
           MLEKLQQ+K++LDKK+++I  WR++S +IFA TFAAVLICSVVA  I APPVAAAL+AA+
Sbjct: 208 MLEKLQQRKHRLDKKVRAIKAWRRVSSIIFATTFAAVLICSVVAAAIAAPPVAAALAAAA 267

Query: 255 SIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLVEKLEIEIEGM 314
           SIPVGSMGKWIDSL K Y++AL+GQKEV+++MQVGT+IAIKD+D+IRVL+ ++E+EI  M
Sbjct: 268 SIPVGSMGKWIDSLLKGYQDALRGQKEVVSAMQVGTFIAIKDLDSIRVLINRVELEISSM 327

Query: 315 LEKADFAIK-EEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVLQRIIKH 368
           ++  +FA + EEA+K GVEE+KKKLEVFMKSVEDLG QAD CSRDI RARTVVLQRII+H
Sbjct: 328 IDCVEFAERDEEAVKFGVEEIKKKLEVFMKSVEDLGEQADRCSRDIRRARTVVLQRIIRH 387

BLAST of HG10000427 vs. ExPASy Swiss-Prot
Match: Q56XQ0 (UPF0496 protein At2g18630 OS=Arabidopsis thaliana OX=3702 GN=At2g18630 PE=2 SV=3)

HSP 1 Score: 370.5 bits (950), Expect = 2.1e-101
Identity = 188/352 (53.41%), Postives = 269/352 (76.42%), Query Frame = 0

Query: 16  TINLDAYEAACRADDDVRSFDKALQARANQVLTTLADGGVEVRALSLDSLKQVTECLLEM 75
           T +L +YE AC  D  + SFD AL  R N+V+  LA  GVE+++LS DSL++VT+CLL+M
Sbjct: 31  TEHLSSYERACSEDPKLESFDSALHERTNRVINKLA-SGVEIKSLSFDSLREVTQCLLDM 90

Query: 76  NQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALENCLKRAQDSQVLILMAV 135
           NQ+VV+VILQ K+DIW NQ+LF LV  YFE++ +T+DFC+ LENCL RA+ SQV+I  AV
Sbjct: 91  NQDVVKVILQDKEDIWNNQDLFSLVNLYFESTAKTMDFCSELENCLNRARRSQVIIQFAV 150

Query: 136 RQFEEEERETQVGPNQFAKTLQELRNFKASGDPFTHDFFKIFHSVYKHQTAMLEKLQQKK 195
            QFEEE  + +    ++ KTL+EL+ FK +G+PFT +FF +F  VYK Q  MLE+L + K
Sbjct: 151 NQFEEENEDKE--NRKYEKTLEELKRFKVAGEPFTKEFFALFDLVYKQQVMMLEELHKLK 210

Query: 196 NKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAPPVAAALSAASSIPVGSMGK 255
            KLDK+L++I TWR++S M+F   F +VLI SVVA  + APPV AA++ A ++PVGS+GK
Sbjct: 211 RKLDKRLRNIKTWRRVSNMVFVTAFVSVLIFSVVAAAVAAPPVVAAIAGALAVPVGSVGK 270

Query: 256 WIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLVEKLEIEIEGMLEKADFAIK 315
           W ++LW  YE  ++GQKE+I S+++GTYI++K+MDNI +LV K+E+EIE +L+KA+FAI 
Sbjct: 271 WCNTLWTKYEKVVRGQKEIITSIRIGTYISVKEMDNISILVRKVEVEIESLLKKAEFAIT 330

Query: 316 EE-ALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVLQRIIKHP 367
           EE  +++ ++E+KKKL+VF +++E+LG  A     D+T+ARTV+LQRII++P
Sbjct: 331 EEKEVRLAIDEIKKKLDVFTETIEELGEHAGKYCSDVTKARTVILQRIIRYP 379

BLAST of HG10000427 vs. ExPASy Swiss-Prot
Match: Q9SYZ8 (UPF0496 protein At4g34330 OS=Arabidopsis thaliana OX=3702 GN=At4g34330 PE=3 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.1e-97
Identity = 202/365 (55.34%), Postives = 265/365 (72.60%), Query Frame = 0

Query: 1   MGN--NHGTKRGSTTVATINLDAYEAACRADDDVRSFDKALQARANQVLTTLADGGVEVR 60
           MGN  +  +K  S    T  L +YEAAC+ D +++SFD  +QAR + V++TLA  GVEVR
Sbjct: 1   MGNKTSRKSKEKSGRNYTTELRSYEAACKEDMEIQSFDTRMQARTSHVISTLAT-GVEVR 60

Query: 61  ALSLDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALE 120
           +LS DSLK V   LL+MNQEV +VIL CKKDIWKNQE+FE VE YFE SL+TLDF  AL+
Sbjct: 61  SLSFDSLKAVIGSLLDMNQEVAKVILDCKKDIWKNQEMFEFVEAYFETSLKTLDFFNALK 120

Query: 121 NCLKRAQDSQVLILMAVRQFEEEERETQVGPNQFAKTLQELRNFKASGDPFTHDFFKIFH 180
             L+  Q + + IL                 N + KTLQEL+ FK +  PF  DFFK+F 
Sbjct: 121 RGLQGVQINHLFIL---------------DGNGYKKTLQELKRFKDADRPFDQDFFKMFQ 180

Query: 181 SVYKHQTAMLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAPPV 240
           SVY  Q  ML+KLQ+++NKLDKKLK I TWRKLS +IF ATFA ++ICSV+A  + AP V
Sbjct: 181 SVYNQQKWMLDKLQRRQNKLDKKLKRIRTWRKLSSIIFMATFATLVICSVLAATMAAPHV 240

Query: 241 AAALSAASSIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLVEK 300
           AAAL+AA+  PVGSMGKWIDSLWK+YEN +KGQ EV +SM VGTY+A++D++NI+ L+++
Sbjct: 241 AAALAAATP-PVGSMGKWIDSLWKNYENEIKGQTEVFSSMYVGTYVAVQDLNNIKDLIKR 300

Query: 301 LEIEIEGMLEKADFAIKEEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVV 360
           L+ EI GM++ A++A +   +K+G+  +K +LEVF K+VE+L +QAD+CS DI RARTV+
Sbjct: 301 LDTEIRGMVKNAEYAGELGPVKIGINAIKTQLEVFKKNVEELEIQADMCSTDIRRARTVI 348

Query: 361 LQRII 364
           LQRII
Sbjct: 361 LQRII 348

BLAST of HG10000427 vs. ExPASy TrEMBL
Match: A0A5D3CTV8 (UPF0496 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G001340 PE=3 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.8e-175
Identity = 333/368 (90.49%), Postives = 349/368 (94.84%), Query Frame = 0

Query: 1   MGNNHGTKRGSTTVATINLDAYEAACRADDDVRSFDKALQARANQVLTTLA-DGGVEVRA 60
           MGN H TKR    +    + AYEAACR DDDVRSFDK LQARANQVLTTLA DGGVEVRA
Sbjct: 1   MGNQHTTKRTPEELQPA-VAAYEAACRVDDDVRSFDKTLQARANQVLTTLADDGGVEVRA 60

Query: 61  LSLDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN 120
           LS DSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN
Sbjct: 61  LSFDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN 120

Query: 121 CLKRAQDSQVLILMAVRQFEEEERETQVGPNQFAKTLQELRNFKASGDPFTHDFFKIFHS 180
           CLKRA+DSQV+ILMAVRQFEE+ERE+Q+GPNQF KTL+ELRNFKASGDPFT +FF+IFHS
Sbjct: 121 CLKRARDSQVMILMAVRQFEEDERESQMGPNQFDKTLRELRNFKASGDPFTDEFFRIFHS 180

Query: 181 VYKHQTAMLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAPPVA 240
           VYKHQTAMLEKLQQKKNKLDKKLKSI+TWRKLSCMIFAATFAAVLICSVVAT+I APPVA
Sbjct: 181 VYKHQTAMLEKLQQKKNKLDKKLKSISTWRKLSCMIFAATFAAVLICSVVATVIMAPPVA 240

Query: 241 AALSAASSIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLVEKL 300
           AALSAASSIP+GSMGKWIDSLWKSYENA+KGQKEVINSMQVGTYIAIKDMDNIR+LVEKL
Sbjct: 241 AALSAASSIPLGSMGKWIDSLWKSYENAVKGQKEVINSMQVGTYIAIKDMDNIRILVEKL 300

Query: 301 EIEIEGMLEKADFAIKEEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVL 360
           EIEI+GMLEKADFAIKEEALKV VEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVL
Sbjct: 301 EIEIKGMLEKADFAIKEEALKVVVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVL 360

Query: 361 QRIIKHPN 368
           QRIIKHPN
Sbjct: 361 QRIIKHPN 367

BLAST of HG10000427 vs. ExPASy TrEMBL
Match: A0A1S3C9S5 (UPF0496 protein At4g34320-like OS=Cucumis melo OX=3656 GN=LOC103498434 PE=3 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.8e-175
Identity = 333/368 (90.49%), Postives = 349/368 (94.84%), Query Frame = 0

Query: 1   MGNNHGTKRGSTTVATINLDAYEAACRADDDVRSFDKALQARANQVLTTLA-DGGVEVRA 60
           MGN H TKR    +    + AYEAACR DDDVRSFDK LQARANQVLTTLA DGGVEVRA
Sbjct: 1   MGNQHTTKRTPEELQPA-VAAYEAACRVDDDVRSFDKTLQARANQVLTTLADDGGVEVRA 60

Query: 61  LSLDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN 120
           LS DSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN
Sbjct: 61  LSFDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN 120

Query: 121 CLKRAQDSQVLILMAVRQFEEEERETQVGPNQFAKTLQELRNFKASGDPFTHDFFKIFHS 180
           CLKRA+DSQV+ILMAVRQFEE+ERE+Q+GPNQF KTL+ELRNFKASGDPFT +FF+IFHS
Sbjct: 121 CLKRARDSQVMILMAVRQFEEDERESQMGPNQFDKTLRELRNFKASGDPFTDEFFRIFHS 180

Query: 181 VYKHQTAMLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAPPVA 240
           VYKHQTAMLEKLQQKKNKLDKKLKSI+TWRKLSCMIFAATFAAVLICSVVAT+I APPVA
Sbjct: 181 VYKHQTAMLEKLQQKKNKLDKKLKSISTWRKLSCMIFAATFAAVLICSVVATVIMAPPVA 240

Query: 241 AALSAASSIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLVEKL 300
           AALSAASSIP+GSMGKWIDSLWKSYENA+KGQKEVINSMQVGTYIAIKDMDNIR+LVEKL
Sbjct: 241 AALSAASSIPLGSMGKWIDSLWKSYENAVKGQKEVINSMQVGTYIAIKDMDNIRILVEKL 300

Query: 301 EIEIEGMLEKADFAIKEEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVL 360
           EIEI+GMLEKADFAIKEEALKV VEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVL
Sbjct: 301 EIEIKGMLEKADFAIKEEALKVVVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVL 360

Query: 361 QRIIKHPN 368
           QRIIKHPN
Sbjct: 361 QRIIKHPN 367

BLAST of HG10000427 vs. ExPASy TrEMBL
Match: A0A0A0LLW3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G238830 PE=3 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 2.0e-174
Identity = 331/367 (90.19%), Postives = 350/367 (95.37%), Query Frame = 0

Query: 1   MGNNHGTKRGSTTVATINLDAYEAACRADDDVRSFDKALQARANQVLTTLA-DGGVEVRA 60
           MGN+H TKR +T      + AYEAACRADDDVRSFDK LQARANQVLTTLA DGGVEVRA
Sbjct: 55  MGNHHTTKR-TTEELQPAVAAYEAACRADDDVRSFDKTLQARANQVLTTLADDGGVEVRA 114

Query: 61  LSLDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN 120
           LS DSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN
Sbjct: 115 LSFDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALEN 174

Query: 121 CLKRAQDSQVLILMAVRQFEEEERETQVGPNQFAKTLQELRNFKASGDPFTHDFFKIFHS 180
           CLKRA+DSQV+ILMAVRQFEE+ERE+Q+GPNQF KTL+ELRNFKASGDPFT +FF+IFHS
Sbjct: 175 CLKRARDSQVMILMAVRQFEEDERESQMGPNQFDKTLRELRNFKASGDPFTDEFFRIFHS 234

Query: 181 VYKHQTAMLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAPPVA 240
           VYKHQTAMLEKLQQKKNKLDKKLKSI+TWRKLSCM+FAATFAAVLICSVVAT+I APPVA
Sbjct: 235 VYKHQTAMLEKLQQKKNKLDKKLKSISTWRKLSCMMFAATFAAVLICSVVATVIMAPPVA 294

Query: 241 AALSAASSIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLVEKL 300
           AALSAASSIP+GSMGKWIDSLWKSYENA+KGQKEVINSMQVGTYIAIKDMDNIR+LVEKL
Sbjct: 295 AALSAASSIPLGSMGKWIDSLWKSYENAVKGQKEVINSMQVGTYIAIKDMDNIRILVEKL 354

Query: 301 EIEIEGMLEKADFAIKEEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVL 360
           EIEIEGMLEKADFAIKE+ALK  VEEMKKKLEVFMKSVEDLGVQADLCSRDITRART+VL
Sbjct: 355 EIEIEGMLEKADFAIKEDALKFVVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTLVL 414

Query: 361 QRIIKHP 367
           QRIIKHP
Sbjct: 415 QRIIKHP 420

BLAST of HG10000427 vs. ExPASy TrEMBL
Match: A0A6J1L534 (UPF0496 protein At4g34320-like OS=Cucurbita maxima OX=3661 GN=LOC111499969 PE=3 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 3.3e-166
Identity = 320/375 (85.33%), Postives = 342/375 (91.20%), Query Frame = 0

Query: 1   MGNNHGTKRGST-------TVATINLDAYEAACRADDDVRSFDKALQARANQVLTTLA-D 60
           MGN+H TKR +         +A  +L  YEAAC AD DVRSFDKALQARANQVLTTLA D
Sbjct: 1   MGNSHATKRSTAAEQPVAINLAAKDLTTYEAACVADADVRSFDKALQARANQVLTTLADD 60

Query: 61  GGVEVRALSLDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLD 120
           GGV+VRALS DSLKQVTECLLEMNQEVVRVILQCKKDIWKNQE+FELVEDYFENSL+TLD
Sbjct: 61  GGVQVRALSFDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQEVFELVEDYFENSLQTLD 120

Query: 121 FCTALENCLKRAQDSQVLILMAVRQFEEEERETQVGPNQFAKTLQELRNFKASGDPFTHD 180
           FCTALENCLKRA+DSQ+LILMA+RQFEEE  ET VG N+F KTLQELRNFKAS DPFTH+
Sbjct: 121 FCTALENCLKRARDSQLLILMALRQFEEE--ETHVGRNRFVKTLQELRNFKASADPFTHE 180

Query: 181 FFKIFHSVYKHQTAMLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATI 240
           FF IFHSVYK QT MLEKLQQKKNKLDKKLKSINTWRKLS MIFAATFAAVLICSVVAT+
Sbjct: 181 FFNIFHSVYKQQTVMLEKLQQKKNKLDKKLKSINTWRKLSSMIFAATFAAVLICSVVATV 240

Query: 241 ITAPPVAAALSAASSIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNI 300
           I APPV AALSAASSIP+GSMGKWIDSLW+SYENALKGQKEVI+SMQVGTYIAIKDMDNI
Sbjct: 241 IAAPPVTAALSAASSIPLGSMGKWIDSLWRSYENALKGQKEVISSMQVGTYIAIKDMDNI 300

Query: 301 RVLVEKLEIEIEGMLEKADFAIKEEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDIT 360
           RVLV KLE E+EGMLEKA+FAI+EEA+KVGVEEMKKKL+VFMKSVEDLGVQADLCS+DIT
Sbjct: 301 RVLVGKLETEMEGMLEKAEFAIEEEAVKVGVEEMKKKLDVFMKSVEDLGVQADLCSKDIT 360

Query: 361 RARTVVLQRIIKHPN 368
           RARTVVLQRIIKHPN
Sbjct: 361 RARTVVLQRIIKHPN 373

BLAST of HG10000427 vs. ExPASy TrEMBL
Match: A0A6J1G5X0 (UPF0496 protein At4g34320-like OS=Cucurbita moschata OX=3662 GN=LOC111451129 PE=3 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.8e-164
Identity = 320/376 (85.11%), Postives = 342/376 (90.96%), Query Frame = 0

Query: 1   MGNNHGTKRGSTTV---ATINLDA-----YEAACRADDDVRSFDKALQARANQVLTTLA- 60
           MGN+H TKR +T     A INL A     YEAAC AD DVRSFDKALQARANQVLTTLA 
Sbjct: 1   MGNSHATKRSTTAAEQPAAINLAAKDLTTYEAACVADADVRSFDKALQARANQVLTTLAD 60

Query: 61  DGGVEVRALSLDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETL 120
           DGGV+VRALS DSLKQVTECLLEMNQEVVRVILQCKKDIWK+QE+FELVEDYFENSL+TL
Sbjct: 61  DGGVQVRALSFDSLKQVTECLLEMNQEVVRVILQCKKDIWKSQEVFELVEDYFENSLQTL 120

Query: 121 DFCTALENCLKRAQDSQVLILMAVRQFEEEERETQVGPNQFAKTLQELRNFKASGDPFTH 180
           DFCTALENCLKRA+DSQ+LILMA+RQFEEE  ET VG N+F KTLQELRNFKAS DPFT 
Sbjct: 121 DFCTALENCLKRARDSQLLILMALRQFEEE--ETHVGRNRFVKTLQELRNFKASADPFTQ 180

Query: 181 DFFKIFHSVYKHQTAMLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVAT 240
           +FF IFHSVYK Q  MLEKLQQKKNKLDKKLKSINTWRKLS MIFAATFAAVLICSVVAT
Sbjct: 181 EFFNIFHSVYKQQMVMLEKLQQKKNKLDKKLKSINTWRKLSSMIFAATFAAVLICSVVAT 240

Query: 241 IITAPPVAAALSAASSIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDN 300
           +I APPV AALSAASSIP+GSMGKWIDSLW+SYENALKGQKEVI+SMQVGTYIAIKDMDN
Sbjct: 241 VIAAPPVTAALSAASSIPLGSMGKWIDSLWRSYENALKGQKEVISSMQVGTYIAIKDMDN 300

Query: 301 IRVLVEKLEIEIEGMLEKADFAIKEEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDI 360
           IRVLV K+E E+EGMLEKA+FAI+EEA+KVGVEEMKKKL+VFMKSVEDLGVQADLCS+DI
Sbjct: 301 IRVLVGKVETEMEGMLEKAEFAIEEEAVKVGVEEMKKKLDVFMKSVEDLGVQADLCSKDI 360

Query: 361 TRARTVVLQRIIKHPN 368
           TRARTVVLQRIIKHPN
Sbjct: 361 TRARTVVLQRIIKHPN 374

BLAST of HG10000427 vs. TAIR 10
Match: AT4G34320.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 477.6 bits (1228), Expect = 8.8e-135
Identity = 249/371 (67.12%), Postives = 309/371 (83.29%), Query Frame = 0

Query: 1   MGNNHGTKRGSTTVATIN----LDAYEAACRADDDVRSFDKALQARANQVLTTLADGGVE 60
           MGN    K   T+  +++    L +Y AAC+AD +++SFD  LQAR + V++TLA  GVE
Sbjct: 1   MGNQTSKKSQETSAKSVHYTTELRSYAAACKADTELQSFDTCLQARTSHVISTLAT-GVE 60

Query: 61  VRALSLDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTA 120
           VRALS DSLK+VT+CLLEMNQEVV+VIL CKKDIWKNQE+FELVEDYFENSL+TLDFC A
Sbjct: 61  VRALSFDSLKEVTQCLLEMNQEVVKVILDCKKDIWKNQEMFELVEDYFENSLKTLDFCAA 120

Query: 121 LENCLKRAQDSQVLILMAVRQFEEEERETQVGPNQFAKTLQELRNFKASGDPFTHDFFKI 180
           LE  L+RA+DS +LIL+A++QFE+E      G N + KTL+EL+NFK +  PF  DFFK+
Sbjct: 121 LEKGLRRARDSHLLILVALQQFEDE--SLVQGGNGYKKTLEELKNFKDAESPFNEDFFKM 180

Query: 181 FHSVYKHQTAMLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAP 240
           F SVYK Q  MLEKLQ +KNKLDKKLK I+TWRKLS +IF ATFA VLICSVVA  + AP
Sbjct: 181 FQSVYKQQMLMLEKLQHRKNKLDKKLKCIHTWRKLSSIIFVATFATVLICSVVAAAMAAP 240

Query: 241 PVAAALSAASSIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLV 300
           PVAAAL+AA+++P+GSMGKWIDSLWK+YENALKGQKEVI+SMQ GT++A+KD+DNIRVL+
Sbjct: 241 PVAAALAAATAVPLGSMGKWIDSLWKNYENALKGQKEVISSMQAGTFVAVKDLDNIRVLI 300

Query: 301 EKLEIEIEGMLEKADFAIKEEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRART 360
           E+LEIEI GM++ A+FA++  A+K+G++++KKKLEVF K+VE+LG QADLCSRDI RART
Sbjct: 301 ERLEIEITGMVKSAEFAVEHNAVKIGIDDIKKKLEVFKKNVEELGTQADLCSRDIRRART 360

Query: 361 VVLQRIIKHPN 368
           V+LQRIIKHPN
Sbjct: 361 VILQRIIKHPN 368

BLAST of HG10000427 vs. TAIR 10
Match: AT2G18630.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 370.5 bits (950), Expect = 1.5e-102
Identity = 188/352 (53.41%), Postives = 269/352 (76.42%), Query Frame = 0

Query: 16  TINLDAYEAACRADDDVRSFDKALQARANQVLTTLADGGVEVRALSLDSLKQVTECLLEM 75
           T +L +YE AC  D  + SFD AL  R N+V+  LA  GVE+++LS DSL++VT+CLL+M
Sbjct: 31  TEHLSSYERACSEDPKLESFDSALHERTNRVINKLA-SGVEIKSLSFDSLREVTQCLLDM 90

Query: 76  NQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALENCLKRAQDSQVLILMAV 135
           NQ+VV+VILQ K+DIW NQ+LF LV  YFE++ +T+DFC+ LENCL RA+ SQV+I  AV
Sbjct: 91  NQDVVKVILQDKEDIWNNQDLFSLVNLYFESTAKTMDFCSELENCLNRARRSQVIIQFAV 150

Query: 136 RQFEEEERETQVGPNQFAKTLQELRNFKASGDPFTHDFFKIFHSVYKHQTAMLEKLQQKK 195
            QFEEE  + +    ++ KTL+EL+ FK +G+PFT +FF +F  VYK Q  MLE+L + K
Sbjct: 151 NQFEEENEDKE--NRKYEKTLEELKRFKVAGEPFTKEFFALFDLVYKQQVMMLEELHKLK 210

Query: 196 NKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAPPVAAALSAASSIPVGSMGK 255
            KLDK+L++I TWR++S M+F   F +VLI SVVA  + APPV AA++ A ++PVGS+GK
Sbjct: 211 RKLDKRLRNIKTWRRVSNMVFVTAFVSVLIFSVVAAAVAAPPVVAAIAGALAVPVGSVGK 270

Query: 256 WIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLVEKLEIEIEGMLEKADFAIK 315
           W ++LW  YE  ++GQKE+I S+++GTYI++K+MDNI +LV K+E+EIE +L+KA+FAI 
Sbjct: 271 WCNTLWTKYEKVVRGQKEIITSIRIGTYISVKEMDNISILVRKVEVEIESLLKKAEFAIT 330

Query: 316 EE-ALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVLQRIIKHP 367
           EE  +++ ++E+KKKL+VF +++E+LG  A     D+T+ARTV+LQRII++P
Sbjct: 331 EEKEVRLAIDEIKKKLDVFTETIEELGEHAGKYCSDVTKARTVILQRIIRYP 379

BLAST of HG10000427 vs. TAIR 10
Match: AT4G34330.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 358.2 bits (918), Expect = 7.8e-99
Identity = 202/365 (55.34%), Postives = 265/365 (72.60%), Query Frame = 0

Query: 1   MGN--NHGTKRGSTTVATINLDAYEAACRADDDVRSFDKALQARANQVLTTLADGGVEVR 60
           MGN  +  +K  S    T  L +YEAAC+ D +++SFD  +QAR + V++TLA  GVEVR
Sbjct: 1   MGNKTSRKSKEKSGRNYTTELRSYEAACKEDMEIQSFDTRMQARTSHVISTLAT-GVEVR 60

Query: 61  ALSLDSLKQVTECLLEMNQEVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALE 120
           +LS DSLK V   LL+MNQEV +VIL CKKDIWKNQE+FE VE YFE SL+TLDF  AL+
Sbjct: 61  SLSFDSLKAVIGSLLDMNQEVAKVILDCKKDIWKNQEMFEFVEAYFETSLKTLDFFNALK 120

Query: 121 NCLKRAQDSQVLILMAVRQFEEEERETQVGPNQFAKTLQELRNFKASGDPFTHDFFKIFH 180
             L+  Q + + IL                 N + KTLQEL+ FK +  PF  DFFK+F 
Sbjct: 121 RGLQGVQINHLFIL---------------DGNGYKKTLQELKRFKDADRPFDQDFFKMFQ 180

Query: 181 SVYKHQTAMLEKLQQKKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAPPV 240
           SVY  Q  ML+KLQ+++NKLDKKLK I TWRKLS +IF ATFA ++ICSV+A  + AP V
Sbjct: 181 SVYNQQKWMLDKLQRRQNKLDKKLKRIRTWRKLSSIIFMATFATLVICSVLAATMAAPHV 240

Query: 241 AAALSAASSIPVGSMGKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLVEK 300
           AAAL+AA+  PVGSMGKWIDSLWK+YEN +KGQ EV +SM VGTY+A++D++NI+ L+++
Sbjct: 241 AAALAAATP-PVGSMGKWIDSLWKNYENEIKGQTEVFSSMYVGTYVAVQDLNNIKDLIKR 300

Query: 301 LEIEIEGMLEKADFAIKEEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVV 360
           L+ EI GM++ A++A +   +K+G+  +K +LEVF K+VE+L +QAD+CS DI RARTV+
Sbjct: 301 LDTEIRGMVKNAEYAGELGPVKIGINAIKTQLEVFKKNVEELEIQADMCSTDIRRARTVI 348

Query: 361 LQRII 364
           LQRII
Sbjct: 361 LQRII 348

BLAST of HG10000427 vs. TAIR 10
Match: AT5G66675.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 353.2 bits (905), Expect = 2.5e-97
Identity = 180/358 (50.28%), Postives = 265/358 (74.02%), Query Frame = 0

Query: 18  NLDAYEAACRADDDVRSFDKALQARANQVLTTLADGGVEVRALSLDSLKQVTECLLEMNQ 77
           +L AY +ACR D D++SFD +L  R N+V+ +LA  G + R+LS D+L +V+ CLLEMNQ
Sbjct: 41  DLTAYNSACREDPDLQSFDSSLHQRTNRVINSLA-SGAQTRSLSFDALIEVSGCLLEMNQ 100

Query: 78  EVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALENCLKRAQDSQVLILMAVRQ 137
           EVVR I++ K+D+W N++L  LV  YF++S++TLDFC A++NC+KRA+  Q+L+  A++Q
Sbjct: 101 EVVRFIIESKEDVWDNKDLTCLVNAYFDSSIKTLDFCNAVDNCVKRARIGQMLLQFALKQ 160

Query: 138 FEEE----ERETQVGPNQFAKTLQELRNFKASGDPFTHDFFKIFHSVYKHQTAMLEKLQQ 197
           FE E     +  + G N++AKTL+EL  FKASGDPF  DFF +  SVY+ Q  +LE L +
Sbjct: 161 FEMESSGSNKSVEPGTNKYAKTLEELNKFKASGDPFDGDFFMLLESVYEQQVMLLEILHK 220

Query: 198 KKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAPPVAAALSAASSIPVGSM 257
           +K KLDKKLK+I  W+K+S ++F   F +VLI SVVA  + APPV  AL+AA ++P+GS+
Sbjct: 221 QKRKLDKKLKNIKYWKKISNVVFVTAFVSVLIFSVVAAAVAAPPVVTALAAALAVPIGSI 280

Query: 258 GKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLVEKLEIEIEGMLEKADFA 317
           GKW + LWK YE A+KGQK+++ SM++G Y+ +KDMDNIRV V+KL+IE+E M++K DFA
Sbjct: 281 GKWCNHLWKKYETAVKGQKDIVLSMRIGAYVTMKDMDNIRVHVDKLKIEMESMMQKVDFA 340

Query: 318 IKEE----ALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVLQRIIKHPN 368
           +KE+    A+++ + E+ KK +VF + +E++G  A  CS++IT ART+VL+ I+  P+
Sbjct: 341 LKEKEEEVAVRLSMHEISKKFDVFTERIEEVGDNATKCSKNITLARTIVLRHILSFPS 397

BLAST of HG10000427 vs. TAIR 10
Match: AT5G66660.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 259.6 bits (662), Expect = 3.8e-69
Identity = 143/357 (40.06%), Postives = 233/357 (65.27%), Query Frame = 0

Query: 18  NLDAYEAACRADDDVRSFDKALQARANQVLTTLADGGVEVRALSLDSLKQVTECLLEMNQ 77
           +L +Y +AC+ D +++SFD +L  R N ++T+LA    E ++L+LDSL +V   LLE+NQ
Sbjct: 41  DLSSYTSACKKDSNLKSFDSSLHQRTNIIITSLA-ARAETQSLNLDSLMEVYGFLLELNQ 100

Query: 78  EVVRVILQCKKDIWKNQELFELVEDYFENSLETLDFCTALENCLKRAQDSQVLILMAVRQ 137
             VRVI++ ++D+WKN++L  LV+ YF+++ +TLDFC  +ENC+KR + SQ++I  AV+Q
Sbjct: 101 NAVRVIIESREDVWKNKDLKSLVDVYFKSTSKTLDFCNTVENCVKRTEISQLIIRFAVKQ 160

Query: 138 FEEEERETQVG----PNQFAKTLQELRNFKASGDPFTHDFFKIFHSVYKHQTAMLEKLQQ 197
           FE E  +T +G      ++ KTL+EL  FKA GDPF  +    F SVY  Q   LE+L++
Sbjct: 161 FEAESVDTDLGGDKKKKKYTKTLEELNKFKAMGDPFDGELVTQFDSVYDQQVLFLEELRK 220

Query: 198 KKNKLDKKLKSINTWRKLSCMIFAATFAAVLICSVVATIITAPPVAAALSAASSIPVGSM 257
           ++ KLDKK +++ T R +S + FA  + +VL+ SVVAT ++APPV  A+++ S+ P+   
Sbjct: 221 QRRKLDKKQRNVKTLRTVSNVFFATAYVSVLVLSVVATTMSAPPVVCAVASGSTAPIEIT 280

Query: 258 GKWIDSLWKSYENALKGQKEVINSMQVGTYIAIKDMDNIRVLVEKLEIEIEGMLEKADFA 317
           GKW   +WK YE A+K Q+ ++ +M+    +  + M NIR  V++L   +  +LE  DFA
Sbjct: 281 GKWFSQMWKKYEKAVKRQRGLVLTMESRVQVNNEAMKNIRSDVDELRSWVSSILETVDFA 340

Query: 318 I----KEEALKVGVEEMKKKLEVFMKSVEDLGVQADLCSRDITRARTVVLQRIIKHP 367
           +    +EEA+ + ++ +KK ++ F + +E++G  A  CS+ I   R +VL+ I+  P
Sbjct: 341 VEREEEEEAMGLAMQGIKKHVDGFTEKMEEVGENAAKCSKFIALGRLLVLEHILGLP 396