HG10000254 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10000254
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Cadmium/zinc-transporting ATPase
Location	Chr09: 2810123 .. 2816221 (+)
RNA-Seq Expression	HG10000254
Synteny	HG10000254

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAACCCTCTCCTTCCCAATTGCATCTTCAAAACCCCGACTCTTATCAATTACAAACCCAATTGCATTTCCTAACTGTTTTCTTCAAACATCTTCCCCTTCTTCTGCAATTCGCCTTCGTAATTTTTCCATTCTCTATCTACGGCGGAGTGTTCTCTGTTCCGCTGCGGAAACTGGTCGTTCGAATCACGATGACCACCACCATGTTCATGATCACCACCACCACCACCACCACCATGGTCACCACCATTGTGATCATGATGTAGAGCTGTTTGGGCCTCAGAAAGCGATGATTGGATTAGCTAAAGCCATTAGGTGGACGGACTTGGCGAGCTATCTAAAGGAGCACTTGCAATTATGTTGTTGCTCTATGGCTTTGTTTGTGGCTGCTGTTGTTTGCCCATATTTGGTGCCGAATCCCATTGTAAAGCCTTTGCAAAATGCTTTAATTGTCATTGCTTATTCTTTAGTCGGGGTATGGATGTCTTCAACTACCACTTTTTGTTCTGTTTTGATTTGAATTGCTTGAATGAATGTGATTGTTGTAAATTTAATCACGAATCCCTTATAGGTTGAAAATTCTTGAAGTTAATGTGGTTTTTATAATTCTAAACCTAGCACTACTACGACTTGTGAGGGTCTAATGATATGCTCGAATCGATTCCATTCTAACTTGAAAATTCAGTGCTGACATGGTGAGATTTTGTAATCATGAACATCCATTATATAGTTTTTCATTTGGACCTCTCAATTATGTATATTAGAGGAGTTGAGAGGCCGTCGTTAATGTGTTTGATCTATTGTTAGGTGAAGTCTTTTTCTAATTATCCTTTACGTCATGTTTTGCTTGATTGGAGTCCCTTTCTTTGGTGGGACTCCTTTTGTGGGCTTGGTTCCTCAATTCCAAAAGCACCTAGTTCCTTCCTCAATTTCGGCTGCCACACATAAGCGGAAATCTGCCTTATTGATCCATAGATATTCATAGTTCTCTATGTCTGATAATGTTTTTTTATTCAGACCAGTGAAAGAGGGAACTTTGTGCGCAAGGTTTCTACAAATCAAATAGCAAAGGACAGTGGCCGAATATCATCGGAAGTTCAAAGCATTATCCGCGTTTGCCCACTTGTTTGAAGAAGTCTTGGAGGGCATGTTTTCTAATGGGCTTAACCTGGTTGTACAGGCGGAGATGTCCTGTTTCAGGCCTGATGGGCTCAAGGACATGATGTTTGTGGCCTAACTTGTGGAAGATAGCATGAAAGTTTCCAAGCTAGCCCAAGAAGATGGGTCGGGTAAGAAAACCAAACTGGAACCCATAGGTTGTTTGAAATTGGCGGGAAAAACACTAAGGTGATGGTGACACGTACAATTACGCCAGCTGAAAAGATCCCATCAAATCGTAGGGCCTATGTTATAGAGATTAGACCTATAAATGTCTTTTAAATTCTAATTTCCAAGCCCGACGTGATAGGGCCTATCTTATCATAGTGATGAGAGATACTCACCTGGTCACTGTTGCAACAACAAAGAACTAAGATTGCTAGTCTTCTCTAACAAATTTTACGAAGTGGAGATGTAGAGTCTACAAGGTGAGATGGAGAACACGGTGAGTTCCATAGTTGAGTTATCCTTGAATTCAGTGGTGGGGTTGACGACTCCAAGGACTATGAAACTCAAAGGGAGAATAGGGGAGCAAAAGATCTTAATTCTGATTGATTGCAAGGAGACCCATAATTTCATTGCTCAAGGATTAGATGAAAACTTGAAGCTTCTGTTGACCAAAACCATGCAATATGGTGTGGTCGTGGGCTAAGGGGAAGCAATGAAAGGAAAAGTAATATGTAAGGTTGTCACGGTTTCGCTTCGGAAAATGATTGTACAAGAGGATTTCCTTATTTGTATTTAGCTGGGAATGTAATGGTTGAGGAAGCAAGGGGCCATGACAGTTGACTGGAAGTCTTTTAGCATGTCGTTTCGAGTGGGAGACTCACACATTGTTTTAGAAGGAGACTTGGCATTAGCTCATATGGAAATATCCCTAAAGATGTTGTAACAGACCTAGGAAGATGAAGATCAAGGATTCTTAGTGGAGTTTCAAGAGTTGGATTTACAAGGGGTTCGAGAGGGGGGAGATTTGGTGACTGATATGGGAATCTCTGAGTTGGATCATTTGTTTACAAAGTATGTGTTGGGGAAAATGATATATATAAGAACTGAATACACAAGCAAACTGATTACATCTCAACTAAATACAGTATACATGTTGTACACAATAATAATGTTCAAACCGAAAAGTAAGCCAAATAATATAATAAGACTTATGAATTTAAATTGTTAAAGGAAATTAGATTTATTCCCAATTATTAGTTTTCTTTGTAGTATTTTTTCCTTTTCTGTATTTTCCTTTATTAGTTTCTTGGAGTCTATATATTCATTGTATTCTGATTATTCAAAATATATCGAATATATACAAATTATTCATAAATCACAACATGGTATCAGAGCCATAAGTCCTAGGGTTTTACACTTTTCGAACTCTGCCTCCATTGACGGTTGGTCGATGGAGGCAAATCCACGAAATCTCGTCACCGTTACACAGCTCCGACATGTTCATCCATATTTGTCACTGTCTTTGTTATGCTTTTAGATCTATGTTGGTGGATCTCTGTCACTGTTCATGTTCTGTCTTCAGATCTGTGTTTGTGGATCTCTGTCCCATTTTCAATTCGGTTGTTTGTGGGTTCTGTTTCAGATCTCTATGTATGTCTTTGATTCCAGTCCAAAAATTCTTCGTGGGTCTTTGTTTTCGGTCCAAAAATGCTTTGTAGGTATCTATCTCCAACCCAAAAATTGTTTGTGGATCTCTGTTTTTGGCTTAGGAGTCTTTCATAGTCTTTGTTTCCAGCCCAGATCTTTCGTGATTTGTTTCCAGCTCAAAAGTTTTTTCAGTATCTGTCATCTGCCATCTTGTCAGTTAACAAACTCAAGTTGGTCACTTTGAGTTTGAGGGAGGGTGTTAAAGGAAATTAGATTTATTCCCAATTATTAGTTTCCTGTGTAGTATTTTTCCTTTTCTGTATTTTCCTTTATTAGTTTCTTAGAGTCTATATATTCATTGTATTCTGATTATTCAAAATATATCGAATATACACAAATTATTCATAAATCACAACAAAAATAACTAAGAATCTCAAGATATTGTGGAGACGTCAATAGGTAATCATAGCACGCTTTTGATAAGCGTCGTTCTGAAAACAATTATTCACTTTGCACCAGATGCAAGCAAACTACAACGTTCGTCTTAATAAGATGCAACAACTAACTCCGGAAGAACTGCAACCTCGAAATACTCGGATCTGGCAGAACTTTTGGATACTTGCTCTCAACTCAACTCAAGATCAAAATAAAGAAAGAAAGGAAAGAACTCTTCAATTTGAAGTGAGTCACAACGAATGAAACAATAAGTTATTTATAGGCTAGCAACTTAGTAACTCCCCAAAATATAAAATAAAATCAAATAAATACAAAATTGAAACATTTGTCAAATTTAACTCAATTGAGTTGTTACTTGACCAAAATCAAACATGATACATTCAAATTGAAGAAACTACACATTTAACTTTTATACATATTAAATTTATACATGAAACATCATCTTAATTTGATGGTTTCAATCCAATTTGAAGTTTCTAACAATGAATGTAATCTGCATAATTAAATCTATTTTTTATGTTAAAAAAATTTCACAAAAGTTTCACTTTCTTTCACTATAAATATCCAACAGTATGAAGATGTGTTTAATATGCCCGACGAACTTCCACCATCTAGATCCATAGATCACCGTATTCAGTTGAAGGATGGGAAACTTTTATTCTGTGGAAAAGGCAGTCGGATTGTAAAGCAATATGGGAATCTGTCCCTCGCATGCAGCGCCAATTTCCCCATTTCCACCTAAGGACAAGGTGTCTTCTGAAGGAGGGGGTATTTTTAGACTCCTTATTGTGAATGCTTATGTTAGGAGCGGTAAATTGGGAAATGGATGTAGTACTAGCCGTTAGTGGGCTGGTCGGCTTGTGTCTTGTTTTGGGTTAGCCTAGACGATTAGGGTTTTTTGTTGCAGAAAGTCTATTTGTACGTTCCTGTTTTTCCTTCTGGTGTTATGTTATGTATTTTGAATAGAACTTTGTTCTAAGAGGAGAGGAGATCGGTGCTCTCTAATCGGCTGGCCAGACTATTTTATAACCCTTGCTATCAGAATATTATTTATAAGAATTGATATTAAGACCAAGAGTCTTTACATATACCTCTCGAAAATTTTCCCAAATGCCTTGCCTCCTAGCTTTCCCTGGGTACAAGTTGGTACTTACCTGATTGCATACTTTACGTGATCATAAAAGTGTAACTATATTTGTGTTCTAGTGGCTAAAAGGAAATGAAGAAATATTGATCAGATCAACCTCCCCCCACAAAAAAAGGGGCAAAATATAAAACAGGGTAGGTCTCACTGTACAAGTATTTATTTACGTATAAAATATCCAAGGATAATAGATAGAAAAACATTTATTTTATGCTACAAGAACCCACATATTCATGTTTTGATATTTTCCTTTCACTCTTTAGGAGCAGTGGTCGACCATAGTATGGGAAAAAACCTCCCTTCCTTTTCTGTCTAGAATTTGGAATACTTTCCGGGTAGAGGACTTGTTGCAACTCTGCATGGTACTAAGGTAATAATTCATAATTACAATTCATACAAGTATGTGCTTCTTTTCCATCCATAAATCCAACATCATCTATCATTCCGTCTGTGAGTTATTCTGATGTAAGTCATGCCTTCATAATAAGGTTGTAGAGTCAGATGCATCCAACAAATAAACACGATTAATATTTGATTCTCTCTGTACGCTATCATTTATCTTGTGCTCGTCCTTTTTTCTGTTGAAGTTTGTGACATAATCTATCTTTGCATGATATTATGATTTATCAACTAAAACGCAACAATTCTACAAAGAAACAAAGCATGTATAATAGAAGTATGAAAGCTTCGTTTTCATCCCCAATCGTTTCTCCAAGAATCTTGAATTCATAAGTTATTTTCTTGAATTCATCAAGATTTTCTTATAGAGTTTTGGCAGCATCCATTTTGAATGTAAAAAATCTCTCACGAAGATACATTTTGTTTGGCAGATCCTTGGAGACATACAGAGCATTAAGCTTGGTCCATATCTTGTGAGAGACTTCTTCATCGATGACCTGCCTTAGCACATTATCGCTTAAATTTAGGATCAATGTTCCAAAGGCAGTCGTCTCTAAGGTTTCTTTTTCTTCGTCTGTCATGGTGGCTGGAAGTTCGTCTGGGTCTTGAAGAGCTCTTAAAGCCTTTTGGTGTCCCAGGATGGCTTTGATTTTGGCTTTCCATAAACCAAAATCAGATTTACCATCAAATTTTTCTATGTCAAACCTTGCCACAGCCATTCTTGATTTATGAAAAAAATCAAGAAGTAGTTTCTTGGTTAGTGTATGAATGCTCTAATACCACTGTTACAATTCAACACACTTTACACAACCGAGAAACGAAGGACAAAGAAGAAAGAAAGACTAAAACCAGAAGATGAGATGAAGAGAAGAAGATAACACACTCAAGCTATATTGGTTTGGCTATATAGCCTACATCCACTTTGAAGGGTAACACACTTTATTACTGAAGAGTTCAAGGAATACAAATGAATCACAATGTGATCTGCAAAAGGATTTTTTCAATCCATACTAAAGAAGATATATGCAACTTATATATATAGACAAAAACAGACACGAACTTAAGAGTTGTTGAAGGATTCACACTGTAACGGAAATAACCAATAGTAACTTTATCGTATTGCATAGTCAGTAACTGGCAAGGTGATTTTGCATTCGTCTTGATTTATTATAGTCGGGAATTGAAGGTGAGAAACTATTGAAAGCATCTCTTGGTTCCTTAGATTTCATCACTTCATGCTACAAATCAGAATCCAAATCAAAAGAGATCAAAGAAGTTGTGAACACATCTTCATATAAAAGCGAGTTTGTTCATGATGCTCTTGCAGTTGATGGGAAGGTATGA

mRNA sequence

ATGGGAACCCTCTCCTTCCCAATTGCATCTTCAAAACCCCGACTCTTATCAATTACAAACCCAATTGCATTTCCTAACTGTTTTCTTCAAACATCTTCCCCTTCTTCTGCAATTCGCCTTCGTAATTTTTCCATTCTCTATCTACGGCGGAGTGTTCTCTGTTCCGCTGCGGAAACTGGTCGTTCGAATCACGATGACCACCACCATGTTCATGATCACCACCACCACCACCACCACCATGGTCACCACCATTGTGATCATGATGTAGAGCTGTTTGGGCCTCAGAAAGCGATGATTGGATTAGCTAAAGCCATTAGGTGGACGGACTTGGCGAGCTATCTAAAGGAGCACTTGCAATTATGTTGTTGCTCTATGGCTTTGTTTGTGGCTGCTGTTGTTTGCCCATATTTGGTGCCGAATCCCATTGTAAAGCCTTTGCAAAATGCTTTAATTGTCATTGCTTATTCTTTAGTCGGGAATTTGGAATACTTTCCGGGTAGAGGACTTGTTGCAACTCTGCATGGTACTAAGTCGGGAATTGAAGGTGAGAAACTATTGAAAGCATCTCTTGGTTCCTTAGATTTCATCACTTCATGCTACAAATCAGAATCCAAATCAAAAGAGATCAAAGAAGTTGTGAACACATCTTCATATAAAAGCGAGTTTGTTCATGATGCTCTTGCAGTTGATGGGAAGGTATGA

Coding sequence (CDS)

ATGGGAACCCTCTCCTTCCCAATTGCATCTTCAAAACCCCGACTCTTATCAATTACAAACCCAATTGCATTTCCTAACTGTTTTCTTCAAACATCTTCCCCTTCTTCTGCAATTCGCCTTCGTAATTTTTCCATTCTCTATCTACGGCGGAGTGTTCTCTGTTCCGCTGCGGAAACTGGTCGTTCGAATCACGATGACCACCACCATGTTCATGATCACCACCACCACCACCACCACCATGGTCACCACCATTGTGATCATGATGTAGAGCTGTTTGGGCCTCAGAAAGCGATGATTGGATTAGCTAAAGCCATTAGGTGGACGGACTTGGCGAGCTATCTAAAGGAGCACTTGCAATTATGTTGTTGCTCTATGGCTTTGTTTGTGGCTGCTGTTGTTTGCCCATATTTGGTGCCGAATCCCATTGTAAAGCCTTTGCAAAATGCTTTAATTGTCATTGCTTATTCTTTAGTCGGGAATTTGGAATACTTTCCGGGTAGAGGACTTGTTGCAACTCTGCATGGTACTAAGTCGGGAATTGAAGGTGAGAAACTATTGAAAGCATCTCTTGGTTCCTTAGATTTCATCACTTCATGCTACAAATCAGAATCCAAATCAAAAGAGATCAAAGAAGTTGTGAACACATCTTCATATAAAAGCGAGTTTGTTCATGATGCTCTTGCAGTTGATGGGAAGGTATGA

Protein sequence

MGTLSFPIASSKPRLLSITNPIAFPNCFLQTSSPSSAIRLRNFSILYLRRSVLCSAAETGRSNHDDHHHVHDHHHHHHHHGHHHCDHDVELFGPQKAMIGLAKAIRWTDLASYLKEHLQLCCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSLVGNLEYFPGRGLVATLHGTKSGIEGEKLLKASLGSLDFITSCYKSESKSKEIKEVVNTSSYKSEFVHDALAVDGKV

Homology

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BLAST of HG10000254 vs. NCBI nr
Match: XP_038900941.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida] >XP_038900942.1 probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida] >XP_038900943.1 probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 235.7 bits (600), Expect = 4.0e-58
Identity = 127/160 (79.38%), Postives = 133/160 (83.12%), Query Frame = 0


Query: 1   MGTLSFPIASSKPRLLSITN-PIAFPNCFLQTSSPSSAIRLRNFSILYLRRSVLCSAAET 60
           MGTLSFPIASSK RL S  N PIAF N FLQ+S  SS  R  NF IL+LRR VLCSAA T
Sbjct: 1   MGTLSFPIASSKSRLSSTPNHPIAFSNPFLQSSFSSSPFRPPNFPILHLRRRVLCSAAVT 60

Query: 61  GRSNHDDHHHVHDHHHHHHHHGHHHCDHDVELFGPQKAMIGLAKAIRWTDLASYLKEHLQ 120
           GRSNHDDH HVHDHHHHHH H HHHCDHDVEL GPQKA+IG AKAIR TDLA+YL+EHLQ
Sbjct: 61  GRSNHDDHQHVHDHHHHHHRHAHHHCDHDVELSGPQKAVIGFAKAIRLTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSLVG 160
           LCCCSMALFVAA VCPY VP PIVKPLQNA IVIA+ LVG
Sbjct: 121 LCCCSMALFVAAAVCPYFVPKPIVKPLQNAFIVIAFPLVG 160

[Close]

BLAST of HG10000254 vs. NCBI nr
Match: XP_038900941.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida] >XP_038900942.1 probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida] >XP_038900943.1 probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 124.0 bits (310), Expect = 1.7e-24
Identity = 74/121 (61.16%), Postives = 84/121 (69.42%), Query Frame = 0


Query: 121 CCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSL--------VGNLEYFPGRGLVAT 180
           CC   AL VAA      +      P+  A  V+ +S+        V NLEYFPGRGL+AT
Sbjct: 483 CCEEEALAVAAA-----MEKGTTHPIGRA--VVDHSMGKDLPSFSVQNLEYFPGRGLIAT 542

Query: 181 LHGTKSGIEGEKLLKASLGSLDFITSCYKSESKSKEIKEVVNTSSYKSEFVHDALAVDGK 234
           LHG++SG EG K LKASLGSLDFITSCY+SESKS+EIKE  NTSSY SEFVH ALAVDGK
Sbjct: 543 LHGSESGTEGRKRLKASLGSLDFITSCYQSESKSREIKEAANTSSYGSEFVHAALAVDGK 596

HSP 2 Score: 224.6 bits (571), Expect = 9.1e-55
Identity = 118/160 (73.75%), Postives = 129/160 (80.62%), Query Frame = 0


Query: 1   MGTLSFPIASSKPRLLSITNPIAFPNCFLQTSSPSSAIRLRNFSILYLRRSVLCSAAETG 60
           MGTLSFPIASSKPRLL   + IAF N  LQ S PSSA  + NF  L+LRR VLC+ A   
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60

Query: 61  RSNHDDHHHVHDHHH-HHHHHGHHHCDHDVELFGPQKAMIGLAKAIRWTDLASYLKEHLQ 120
            SNHD+HH VH+HHH HHHHHGHHHCDHD EL GPQ+A+IG AKAIRWTDLA+YL+EHLQ
Sbjct: 61  HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSLVG 160
           LCCCSMALFVAA VCPYLVP P VKP QNALI IA+ LVG
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVG 160

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BLAST of HG10000254 vs. NCBI nr
Match: XP_022947718.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 113.6 bits (283), Expect = 2.3e-21
Identity = 72/121 (59.50%), Postives = 81/121 (66.94%), Query Frame = 0


Query: 121 CCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSL--------VGNLEYFPGRGLVAT 180
           CC   AL VAA      +      P+  A  V+ +SL        V NLEYFPGRGLVA 
Sbjct: 483 CCEEEALAVAAA-----MEKGTTHPIGRA--VVDHSLGKDLPSFSVQNLEYFPGRGLVAM 542

Query: 181 LHGTKSGIEGEKLLKASLGSLDFITSCYKSESKSKEIKEVVNTSSYKSEFVHDALAVDGK 234
           L G +SG EG KLLKASLGSLDFITS YKS++KSK+IKE  +TSSY SEFVH ALAVDGK
Sbjct: 543 LQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVDGK 596

HSP 2 Score: 224.6 bits (571), Expect = 9.1e-55
Identity = 118/160 (73.75%), Postives = 129/160 (80.62%), Query Frame = 0


Query: 1   MGTLSFPIASSKPRLLSITNPIAFPNCFLQTSSPSSAIRLRNFSILYLRRSVLCSAAETG 60
           MGTLSFPIASSKPRLL   + IAF N  LQ S PSSA  + NF  L+LRR VLC+ A   
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60

Query: 61  RSNHDDHHHVHD-HHHHHHHHGHHHCDHDVELFGPQKAMIGLAKAIRWTDLASYLKEHLQ 120
            SNHD+HH VH+ HHHHHHHHGHHHCDHD EL GPQ+A+IG AKAIRWTDLA+YL+EHLQ
Sbjct: 61  HSNHDEHHRVHNHHHHHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSLVG 160
           LCCCSMALFVAA VCPYLVP P VKP QNALI IA+ LVG
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVG 160

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BLAST of HG10000254 vs. NCBI nr
Match: KAG6605188.1 (putative cadmium/zinc-transporting ATPase HMA1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 84.7 bits (208), Expect = 1.1e-12
Identity = 45/64 (70.31%), Postives = 51/64 (79.69%), Query Frame = 0


Query: 170 VATLHGTKSGIEGEKLLKASLGSLDFITSCYKSESKSKEIKEVVNTSSYKSEFVHDALAV 229
           +A     + G EG KLLKASLGSLDFITS YKS++KSK+IKE  +TSSY SEFVH ALAV
Sbjct: 489 LAVAAAMEKGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAV 548

Query: 230 DGKV 234
           DGKV
Sbjct: 549 DGKV 552

HSP 2 Score: 223.8 bits (569), Expect = 1.6e-54
Identity = 118/160 (73.75%), Postives = 128/160 (80.00%), Query Frame = 0


Query: 1   MGTLSFPIASSKPRLLSITNPIAFPNCFLQTSSPSSAIRLRNFSILYLRRSVLCSAAETG 60
           MGTLSFPIASSKPRLL   + IAF N  LQ S PSSA  + NF  L+LRR VLC+ A   
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPYLHLRRLVLCATAAND 60

Query: 61  RSNHDDHHHVHDHHH-HHHHHGHHHCDHDVELFGPQKAMIGLAKAIRWTDLASYLKEHLQ 120
            SNHD+HH VH HHH HHHHHGHHHCDHD EL GPQ+A+IG AKAIRWTDLA+YL+EHLQ
Sbjct: 61  HSNHDEHHRVHYHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSLVG 160
           LCCCSMALFVAA VCPYLVP P VKP QNALI IA+ LVG
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVG 160

[Close]

BLAST of HG10000254 vs. NCBI nr
Match: XP_023006942.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 114.4 bits (285), Expect = 1.3e-21
Identity = 72/121 (59.50%), Postives = 82/121 (67.77%), Query Frame = 0


Query: 121 CCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSL--------VGNLEYFPGRGLVAT 180
           CC   AL VAA      +      P+  A  V+ +SL        V NLEYFPGRGLVAT
Sbjct: 483 CCEEEALAVAAA-----MEKGTTHPIGRA--VVDHSLGKDLPSFSVQNLEYFPGRGLVAT 542

Query: 181 LHGTKSGIEGEKLLKASLGSLDFITSCYKSESKSKEIKEVVNTSSYKSEFVHDALAVDGK 234
           L G +SG EG KLLKASLGSLDFITS Y+S++KSK+IKE  +TSSY SEFVH ALAVDGK
Sbjct: 543 LQGIESGTEGGKLLKASLGSLDFITSLYESDTKSKKIKEAASTSSYGSEFVHAALAVDGK 596

HSP 2 Score: 216.9 bits (551), Expect = 1.9e-52
Identity = 121/169 (71.60%), Postives = 129/169 (76.33%), Query Frame = 0


Query: 1   MGTLSFPIASSKPRLLSITNPI-AFPNCFLQTSSPSSAIRLRNFSILYLRRSVLCSAAET 60
           MGTLSFPI SSK  LLS  NP+  F + FLQ+S  SS    RNF IL+LRR VLCSAA  
Sbjct: 1   MGTLSFPIPSSKSHLLSNPNPVPLFRHPFLQSSFSSSPFPSRNFPILHLRRRVLCSAAAA 60

Query: 61  GRSNHDDHHHVHD---------HHHHHHHHGHHHCDHDVELFGPQKAMIGLAKAIRWTDL 120
           GRSNHDDHHHVHD         HHHHHHHH HHH DHDVEL GPQKA+IG AKA RWTDL
Sbjct: 61  GRSNHDDHHHVHDHNHGHHHHHHHHHHHHHHHHHGDHDVELSGPQKAVIGFAKATRWTDL 120

Query: 121 ASYLKEHLQLCCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSLVG 160
           A+YL+E LQLCCCSMALFVAA VCPYLVP PIVKPLQ A IVI + LVG
Sbjct: 121 ANYLRESLQLCCCSMALFVAAAVCPYLVPKPIVKPLQIAFIVIGFPLVG 169

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BLAST of HG10000254 vs. ExPASy Swiss-Prot
Match: Q9M3H5 (Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HMA1 PE=2 SV=2)

HSP 1 Score: 98.6 bits (244), Expect = 9.9e-20
Identity = 60/115 (52.17%), Postives = 74/115 (64.35%), Query Frame = 0


Query: 50  RSVLCSAAETGRSNHDDHHH--VHDHHHHHHHHGHHHCDHDVELFG---PQKAMIGLAKA 109
           RS+   A E    +H DHHH    DHH+HHHHH  H C   VEL     PQK + G AKA
Sbjct: 57  RSIRLRAVE---DHHHDHHHDDEQDHHNHHHHHHQHGC-CSVELKAESKPQKMLFGFAKA 116

Query: 110 IRWTDLASYLKEHLQLCCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSLVG 160
           I W  LA+YL+EHL LCC + A+F+AA VCPYL P P +K LQNA +++ + LVG
Sbjct: 117 IGWVRLANYLREHLHLCCSAAAMFLAAAVCPYLAPEPYIKSLQNAFMIVGFPLVG 167

HSP 2 Score: 92.0 bits (227), Expect = 9.3e-18
Identity = 46/76 (60.53%), Postives = 59/76 (77.63%), Query Frame = 0


Query: 158 VGNLEYFPGRGLVATLHGTKSGIEGEKLLKASLGSLDFITSCYKSESKSKEIKEVVNTSS 217
           V + EYFPGRGL AT++G K+  E  +L KASLGS++FITS +KSE +SK+IK+ VN SS
Sbjct: 527 VESFEYFPGRGLTATVNGVKTVAEESRLRKASLGSIEFITSLFKSEDESKQIKDAVNASS 586

Query: 218 YKSEFVHDALAVDGKV 234
           Y  +FVH AL+VD KV
Sbjct: 587 YGKDFVHAALSVDQKV 602

[Close]

BLAST of HG10000254 vs. ExPASy TrEMBL
Match: A0A6J1G7D7 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111451500 PE=3 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 4.4e-55
Identity = 118/160 (73.75%), Postives = 129/160 (80.62%), Query Frame = 0


Query: 1   MGTLSFPIASSKPRLLSITNPIAFPNCFLQTSSPSSAIRLRNFSILYLRRSVLCSAAETG 60
           MGTLSFPIASSKPRLL   + IAF N  LQ S PSSA  + NF  L+LRR VLC+ A   
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60

Query: 61  RSNHDDHHHVHDHHH-HHHHHGHHHCDHDVELFGPQKAMIGLAKAIRWTDLASYLKEHLQ 120
            SNHD+HH VH+HHH HHHHHGHHHCDHD EL GPQ+A+IG AKAIRWTDLA+YL+EHLQ
Sbjct: 61  HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSLVG 160
           LCCCSMALFVAA VCPYLVP P VKP QNALI IA+ LVG
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVG 160

[Close]

BLAST of HG10000254 vs. ExPASy TrEMBL
Match: A0A6J1G7D7 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111451500 PE=3 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 1.1e-21
Identity = 72/121 (59.50%), Postives = 81/121 (66.94%), Query Frame = 0


Query: 121 CCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSL--------VGNLEYFPGRGLVAT 180
           CC   AL VAA      +      P+  A  V+ +SL        V NLEYFPGRGLVA 
Sbjct: 483 CCEEEALAVAAA-----MEKGTTHPIGRA--VVDHSLGKDLPSFSVQNLEYFPGRGLVAM 542

Query: 181 LHGTKSGIEGEKLLKASLGSLDFITSCYKSESKSKEIKEVVNTSSYKSEFVHDALAVDGK 234
           L G +SG EG KLLKASLGSLDFITS YKS++KSK+IKE  +TSSY SEFVH ALAVDGK
Sbjct: 543 LQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVDGK 596

HSP 2 Score: 223.8 bits (569), Expect = 7.5e-55
Identity = 118/160 (73.75%), Postives = 128/160 (80.00%), Query Frame = 0


Query: 1   MGTLSFPIASSKPRLLSITNPIAFPNCFLQTSSPSSAIRLRNFSILYLRRSVLCSAAETG 60
           MGTLSFPIASSKPRLL   + IAF N  LQ S PSSA  + NF  L+LRR VLC+ A   
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPYLHLRRLVLCATAAND 60

Query: 61  RSNHDDHHHVHDHHH-HHHHHGHHHCDHDVELFGPQKAMIGLAKAIRWTDLASYLKEHLQ 120
            SNHD+HH VH HHH HHHHHGHHHCDHD EL GPQ+A+IG AKAIRWTDLA+YL+EHLQ
Sbjct: 61  HSNHDEHHRVHYHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSLVG 160
           LCCCSMALFVAA VCPYLVP P VKP QNALI IA+ LVG
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVG 160

[Close]

BLAST of HG10000254 vs. ExPASy TrEMBL
Match: A0A6J1KX68 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499582 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 6.4e-22
Identity = 72/121 (59.50%), Postives = 82/121 (67.77%), Query Frame = 0


Query: 121 CCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSL--------VGNLEYFPGRGLVAT 180
           CC   AL VAA      +      P+  A  V+ +SL        V NLEYFPGRGLVAT
Sbjct: 483 CCEEEALAVAAA-----MEKGTTHPIGRA--VVDHSLGKDLPSFSVQNLEYFPGRGLVAT 542

Query: 181 LHGTKSGIEGEKLLKASLGSLDFITSCYKSESKSKEIKEVVNTSSYKSEFVHDALAVDGK 234
           L G +SG EG KLLKASLGSLDFITS Y+S++KSK+IKE  +TSSY SEFVH ALAVDGK
Sbjct: 543 LQGIESGTEGGKLLKASLGSLDFITSLYESDTKSKKIKEAASTSSYGSEFVHAALAVDGK 596

HSP 2 Score: 216.9 bits (551), Expect = 9.2e-53
Identity = 121/169 (71.60%), Postives = 129/169 (76.33%), Query Frame = 0


Query: 1   MGTLSFPIASSKPRLLSITNPI-AFPNCFLQTSSPSSAIRLRNFSILYLRRSVLCSAAET 60
           MGTLSFPI SSK  LLS  NP+  F + FLQ+S  SS    RNF IL+LRR VLCSAA  
Sbjct: 1   MGTLSFPIPSSKSHLLSNPNPVPLFRHPFLQSSFSSSPFPSRNFPILHLRRRVLCSAAAA 60

Query: 61  GRSNHDDHHHVHD---------HHHHHHHHGHHHCDHDVELFGPQKAMIGLAKAIRWTDL 120
           GRSNHDDHHHVHD         HHHHHHHH HHH DHDVEL GPQKA+IG AKA RWTDL
Sbjct: 61  GRSNHDDHHHVHDHNHGHHHHHHHHHHHHHHHHHGDHDVELSGPQKAVIGFAKATRWTDL 120

Query: 121 ASYLKEHLQLCCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSLVG 160
           A+YL+E LQLCCCSMALFVAA VCPYLVP PIVKPLQ A IVI + LVG
Sbjct: 121 ANYLRESLQLCCCSMALFVAAAVCPYLVPKPIVKPLQIAFIVIGFPLVG 169

[Close]

BLAST of HG10000254 vs. ExPASy TrEMBL
Match: A0A5A7VJB5 (Putative cadmium/zinc-transporting ATPase HMA1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold21G005290 PE=3 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.3e-22
Identity = 73/119 (61.34%), Postives = 81/119 (68.07%), Query Frame = 0


Query: 121 CCCSMALFVAAVVCPYLVPNPIVKPLQNALI------VIAYSLVGNLEYFPGRGLVATLH 180
           CC   AL VAA      +      P+  A++      V+    V +LEYFPGRGLVATLH
Sbjct: 492 CCEDEALAVAAA-----MEKGTTHPIGRAVVGHSMGKVLPSFSVQSLEYFPGRGLVATLH 551

Query: 181 GTKSGIEGEKLLKASLGSLDFITSCYKSESKSKEIKEVVNTSSYKSEFVHDALAVDGKV 234
           GT+S   G KLLKASLGSLDFITSCYKS +KSKEIKE  NTSSY SEFVH ALAVDGKV
Sbjct: 552 GTES---GGKLLKASLGSLDFITSCYKSGTKSKEIKEAANTSSYGSEFVHAALAVDGKV 602

HSP 2 Score: 212.6 bits (540), Expect = 1.7e-51
Identity = 120/167 (71.86%), Postives = 126/167 (75.45%), Query Frame = 0


Query: 1   MGTLSFPIASSKPRLLSITNPI-AFPNCFLQTSSPSSAIRLRNFSILYLRRSVLCSAAET 60
           MGTLSFPI SSK  LLS  NP+  F + FLQ+S  SS    RNF IL LRR VLCSAA  
Sbjct: 1   MGTLSFPIPSSKSHLLSNPNPVPLFRHPFLQSSFSSSPFPSRNFPILRLRRRVLCSAAAA 60

Query: 61  GRSNHDDHHHVHD-------HHHHHHHHGHHHCDHDVELFGPQKAMIGLAKAIRWTDLAS 120
           GRSNHDDHHHVHD       HHHHHHHH  HH DHDVEL GPQKA+IG AKA RWTDLA 
Sbjct: 61  GRSNHDDHHHVHDHNHGHHHHHHHHHHHHRHHGDHDVELSGPQKAVIGFAKATRWTDLAK 120

Query: 121 YLKEHLQLCCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSLVG 160
           YL+E LQLCCCSMALFVAA VCPYLVP PIVKPLQ A IVI + LVG
Sbjct: 121 YLRESLQLCCCSMALFVAAAVCPYLVPKPIVKPLQIAFIVIGFPLVG 167

[Close]

BLAST of HG10000254 vs. ExPASy TrEMBL
Match: A0A5D3BGM3 (Putative cadmium/zinc-transporting ATPase HMA1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G001560 PE=3 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.3e-22
Identity = 73/119 (61.34%), Postives = 81/119 (68.07%), Query Frame = 0


Query: 121 CCCSMALFVAAVVCPYLVPNPIVKPLQNALI------VIAYSLVGNLEYFPGRGLVATLH 180
           CC   AL VAA      +      P+  A++      V+    V +LEYFPGRGLVATLH
Sbjct: 490 CCEDEALAVAAA-----MEKGTTHPIGRAVVGHSMGKVLPSFSVQSLEYFPGRGLVATLH 549

Query: 181 GTKSGIEGEKLLKASLGSLDFITSCYKSESKSKEIKEVVNTSSYKSEFVHDALAVDGKV 234
           GT+S   G KLLKASLGSLDFITSCYKS +KSKEIKE  NTSSY SEFVH ALAVDGKV
Sbjct: 550 GTES---GGKLLKASLGSLDFITSCYKSGTKSKEIKEAANTSSYGSEFVHAALAVDGKV 600

HSP 2 Score: 212.6 bits (540), Expect = 1.7e-51
Identity = 120/167 (71.86%), Postives = 126/167 (75.45%), Query Frame = 0


Query: 1   MGTLSFPIASSKPRLLSITNPI-AFPNCFLQTSSPSSAIRLRNFSILYLRRSVLCSAAET 60
           MGTLSFPI SSK  LLS  NP+  F + FLQ+S  SS    RNF IL LRR VLCSAA  
Sbjct: 1   MGTLSFPIPSSKSHLLSNPNPVPLFRHPFLQSSFSSSPFPSRNFPILRLRRRVLCSAAAA 60

Query: 61  GRSNHDDHHHVHD-------HHHHHHHHGHHHCDHDVELFGPQKAMIGLAKAIRWTDLAS 120
           GRSNHDDHHHVHD       HHHHHHHH  HH DHDVEL GPQKA+IG AKA RWTDLA 
Sbjct: 61  GRSNHDDHHHVHDHNHGHHHHHHHHHHHHRHHGDHDVELSGPQKAVIGFAKATRWTDLAK 120

Query: 121 YLKEHLQLCCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSLVG 160
           YL+E LQLCCCSMALFVAA VCPYLVP PIVKPLQ A IVI + LVG
Sbjct: 121 YLRESLQLCCCSMALFVAAAVCPYLVPKPIVKPLQIAFIVIGFPLVG 167

[Close]

BLAST of HG10000254 vs. TAIR 10
Match: AT4G37270.1 (heavy metal atpase 1 )

HSP 1 Score: 98.6 bits (244), Expect = 7.0e-21
Identity = 60/115 (52.17%), Postives = 74/115 (64.35%), Query Frame = 0


Query: 50  RSVLCSAAETGRSNHDDHHH--VHDHHHHHHHHGHHHCDHDVELFG---PQKAMIGLAKA 109
           RS+   A E    +H DHHH    DHH+HHHHH  H C   VEL     PQK + G AKA
Sbjct: 57  RSIRLRAVE---DHHHDHHHDDEQDHHNHHHHHHQHGC-CSVELKAESKPQKMLFGFAKA 116

Query: 110 IRWTDLASYLKEHLQLCCCSMALFVAAVVCPYLVPNPIVKPLQNALIVIAYSLVG 160
           I W  LA+YL+EHL LCC + A+F+AA VCPYL P P +K LQNA +++ + LVG
Sbjct: 117 IGWVRLANYLREHLHLCCSAAAMFLAAAVCPYLAPEPYIKSLQNAFMIVGFPLVG 167

HSP 2 Score: 92.0 bits (227), Expect = 6.6e-19
Identity = 46/76 (60.53%), Postives = 59/76 (77.63%), Query Frame = 0


Query: 158 VGNLEYFPGRGLVATLHGTKSGIEGEKLLKASLGSLDFITSCYKSESKSKEIKEVVNTSS 217
           V + EYFPGRGL AT++G K+  E  +L KASLGS++FITS +KSE +SK+IK+ VN SS
Sbjct: 527 VESFEYFPGRGLTATVNGVKTVAEESRLRKASLGSIEFITSLFKSEDESKQIKDAVNASS 586

Query: 218 YKSEFVHDALAVDGKV 234
           Y  +FVH AL+VD KV
Sbjct: 587 YGKDFVHAALSVDQKV 602

The following BLAST results are available for this feature:

BLAST of HG10000254 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900941.1	4.0e-58	79.38	probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Beninc...	[more]
XP_038900941.1	1.7e-24	61.16	probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Beninc...	[more]
XP_022947718.1	2.3e-21	59.50	probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita moschat...	[more]
KAG6605188.1	1.1e-12	70.31	putative cadmium/zinc-transporting ATPase HMA1, chloroplastic, partial [Cucurbit...	[more]
XP_023006942.1	1.3e-21	59.50	probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita maxima]	[more]

Pages

BLAST of HG10000254 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M3H5	9.9e-20	52.17	Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Arabidopsis tha...	[more]

BLAST of HG10000254 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G7D7	4.4e-55	73.75	probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita mosch...	[more]
A0A6J1G7D7	1.1e-21	59.50	probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita mosch...	[more]
A0A6J1KX68	6.4e-22	59.50	probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita maxim...	[more]
A0A5A7VJB5	1.3e-22	61.34	Putative cadmium/zinc-transporting ATPase HMA1 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A5D3BGM3	1.3e-22	61.34	Putative cadmium/zinc-transporting ATPase HMA1 OS=Cucumis melo var. makuwa OX=11...	[more]

Pages

BLAST of HG10000254 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37270.1	7.0e-21	52.17	heavy metal atpase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR43079:SF3	BNAA09G42040D PROTEIN	coord: 49..159
None	No IPR available	PANTHER	PTHR43079:SF3	BNAA09G42040D PROTEIN	coord: 158..233
None	No IPR available	PANTHER	PTHR43079	PROBABLE CADMIUM/ZINC-TRANSPORTING ATPASE HMA1	coord: 49..159
None	No IPR available	PANTHER	PTHR43079	PROBABLE CADMIUM/ZINC-TRANSPORTING ATPASE HMA1	coord: 158..233

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10000254.1	HG10000254.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098655	cation transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0019829	ATPase-coupled cation transmembrane transporter activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0046872	metal ion binding