HG10000065 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10000065 vs. NCBI nr
Match: XP_004140068.1 (uncharacterized membrane protein At3g27390 [Cucumis sativus] >KAE8646810.1 hypothetical protein Csa_005240 [Cucumis sativus])

HSP 1 Score: 1033.9 bits (2672), Expect = 5.9e-298
Identity = 518/585 (88.55%), Postives = 554/585 (94.70%), Query Frame = 0

Query: 55  MRVPVGFFPNLWSFVSFLPFFFFLLVLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPAHFF 114
           MRVP GFFP LWSF+SFLPFF  LL+LG LKA IVAPI+AAI+VI NSSVI+GLFPAHFF
Sbjct: 1   MRVPFGFFPYLWSFISFLPFFLLLLLLGLLKAAIVAPISAAIIVICNSSVILGLFPAHFF 60

Query: 115 WTFFCFARTKRLGIVLKTAVLVFLPLPLILWPVVGVVGSLLGGIGYGFFVPLIATFEAVG 174
           WTFFCFARTK+LGI+LKT VLVFLPLPLILWPVVG+VGSLLGGIGYGFFVPLIATFEAVG
Sbjct: 61  WTFFCFARTKKLGIILKTVVLVFLPLPLILWPVVGIVGSLLGGIGYGFFVPLIATFEAVG 120

Query: 175 AGVTDKLYHCLADGCLSTTKASCLVVMDFTDFCFHSYFSYMDELGELMHSDEKPMEVKLS 234
           AGVTDKLYHCLADGCLST KASCL+VMDFTDFCF+SYFSYMDELGELMHSDEKPMEVKLS
Sbjct: 121 AGVTDKLYHCLADGCLSTIKASCLIVMDFTDFCFNSYFSYMDELGELMHSDEKPMEVKLS 180

Query: 235 RLPCCLLASLIGVSVDLLFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG 294
           RLPCCLLASLIGV VD +FITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG
Sbjct: 181 RLPCCLLASLIGVPVDFIFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG 240

Query: 295 LAIILWPLAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSMFDEYVNDLL 354
           LAIILWPLAVVGAVISA++SS FLGLYAGVIVHQEDSF+LG+AYVLSVVSMFDEYVNDLL
Sbjct: 241 LAIILWPLAVVGAVISAIISSLFLGLYAGVIVHQEDSFQLGIAYVLSVVSMFDEYVNDLL 300

Query: 355 YLREGSCIPRPKYRRNVNSDLKREHRSDDKNDGRSIRDGSNNHRLVSEQSRTLKWVIHQY 414
           YLREGSCIPRPKYRRN +SDLKREHRSD+KND RS+++G NNH+LVSEQSRTLKWVI  Y
Sbjct: 301 YLREGSCIPRPKYRRNTSSDLKREHRSDNKNDARSMKNGENNHKLVSEQSRTLKWVIQHY 360

Query: 415 KPVQVWDWLFKSCEVNGRILLQDGLISMEDIEECILKGNCKKLSIKLPAWCILQCLLSSA 474
           KPV VWDWLF SCEVNGR+LLQDGLI+ EDIEECILKGNCKKLSIKLPAWCILQCLLSSA
Sbjct: 361 KPVLVWDWLFMSCEVNGRLLLQDGLITTEDIEECILKGNCKKLSIKLPAWCILQCLLSSA 420

Query: 475 KSNSPGLIISDGVELTKTNLPRDTMFEWFLGPLLIMKEQIKRLHLEESEEICLRILIMRC 534
           KSNSPGL+IS+ VELT+TNLPRDTMFEWFLGPLL+MKEQIKRLHLEE+EEICLRILIM+C
Sbjct: 421 KSNSPGLVISNNVELTRTNLPRDTMFEWFLGPLLLMKEQIKRLHLEENEEICLRILIMKC 480

Query: 535 RNEIPEDWDDFGFPSSDTVRRAQLQGIFRRLQGIVNSMSRIPTFRRRFRSLIKVLYVEAL 594
           RNE PEDWD+FGFPS++TVRRAQLQ IFRRLQGIVNS+SRIP+FRRRFRSLIKVLYVEAL
Sbjct: 481 RNEKPEDWDNFGFPSNNTVRRAQLQAIFRRLQGIVNSVSRIPSFRRRFRSLIKVLYVEAL 540

Query: 595 QMGSSANATRIRNGSKRLGRSRDGRNREEETANTIRKAPNNTDVV 640
           Q GSSA+ATRIRNGSK LG  RDGRN EEETANTI+K PN TD V
Sbjct: 541 QKGSSADATRIRNGSKPLGNLRDGRNGEEETANTIQKPPNTTDDV 585

BLAST of HG10000065 vs. NCBI nr
Match: XP_008448134.1 (PREDICTED: uncharacterized membrane protein At3g27390 [Cucumis melo])

HSP 1 Score: 1033.5 bits (2671), Expect = 7.8e-298
Identity = 520/588 (88.44%), Postives = 558/588 (94.90%), Query Frame = 0

Query: 52  ITLMRVPVGFFPNLWSFVSFLPFFFFLLVLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPA 111
           +TL+RVP GFFPNLW F+SFLPFFF LL+LGFLKAVIVAPI+AAI+VI NSSVIVGLFPA
Sbjct: 1   MTLVRVPFGFFPNLWRFISFLPFFFLLLLLGFLKAVIVAPISAAIIVICNSSVIVGLFPA 60

Query: 112 HFFWTFFCFARTKRLGIVLKTAVLVFLPLPLILWPVVGVVGSLLGGIGYGFFVPLIATFE 171
           HFFWTFFCFARTK+LGIV KT VLVFLPLPLILWP VG+VGSL+GGIGYGFFVPLIATFE
Sbjct: 61  HFFWTFFCFARTKKLGIVFKTVVLVFLPLPLILWPAVGIVGSLVGGIGYGFFVPLIATFE 120

Query: 172 AVGAGVTDKLYHCLADGCLSTTKASCLVVMDFTDFCFHSYFSYMDELGELMHSDEKPMEV 231
           AVGAGVTDKLYHCLADGCLST KASCLVVMDFTDFCF+SYFSYMDELGELM SDEKPMEV
Sbjct: 121 AVGAGVTDKLYHCLADGCLSTIKASCLVVMDFTDFCFNSYFSYMDELGELMLSDEKPMEV 180

Query: 232 KLSRLPCCLLASLIGVSVDLLFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVP 291
           KLSRLP CLLASLIG+ VD +FITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVP
Sbjct: 181 KLSRLPYCLLASLIGLPVDFIFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVP 240

Query: 292 FAGLAIILWPLAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSMFDEYVN 351
           FAGLAIILWPLAVVGAVISA++SSFFLGLYAGVIVHQEDSF+LGLAY+LSVVSMFDEYVN
Sbjct: 241 FAGLAIILWPLAVVGAVISAIISSFFLGLYAGVIVHQEDSFQLGLAYILSVVSMFDEYVN 300

Query: 352 DLLYLREGSCIPRPKYRRNVNSDLKREHRSDDKNDGRSIRDGSNNHRLVSEQSRTLKWVI 411
           DLLYLREGSCIPRPKYRRN++SDLK +HRSDDKND RS+ +G+NNH LVSEQSRTLKWVI
Sbjct: 301 DLLYLREGSCIPRPKYRRNMSSDLKMKHRSDDKNDARSMGNGANNHNLVSEQSRTLKWVI 360

Query: 412 HQYKPVQVWDWLFKSCEVNGRILLQDGLISMEDIEECILKGNCKKLSIKLPAWCILQCLL 471
             YKPV VWDWLF SCEVNGR+LLQDGLI+ EDIEECILKGN KKLSI+LPAWCILQCLL
Sbjct: 361 QHYKPVLVWDWLFMSCEVNGRLLLQDGLITTEDIEECILKGNSKKLSIRLPAWCILQCLL 420

Query: 472 SSAKSNSPGLIISDGVELTKTNLPRDTMFEWFLGPLLIMKEQIKRLHLEESEEICLRILI 531
           SSAKSNSPGL+IS+GVELT+TNLPRDTMFEWFLGPLL+MKEQIKRLHLEE+EEICLRILI
Sbjct: 421 SSAKSNSPGLVISNGVELTRTNLPRDTMFEWFLGPLLLMKEQIKRLHLEENEEICLRILI 480

Query: 532 MRCRNEIPEDWDDFGFPSSDTVRRAQLQGIFRRLQGIVNSMSRIPTFRRRFRSLIKVLYV 591
           MRCRNEIPEDWD+FGFPS D VRRAQLQ IFRRLQGIVNS+SRIP+FRRRFRSLIKVLYV
Sbjct: 481 MRCRNEIPEDWDNFGFPSLDRVRRAQLQAIFRRLQGIVNSVSRIPSFRRRFRSLIKVLYV 540

Query: 592 EALQMGSSANATRIRNGSKRLGRSRDGRNREEETANTIRKAPNNTDVV 640
           EALQ GSSA+ATRIRNGS+ LG  RDGRN EEETANT++KAPN+TDVV
Sbjct: 541 EALQKGSSADATRIRNGSEPLGNRRDGRNGEEETANTVQKAPNSTDVV 588

BLAST of HG10000065 vs. NCBI nr
Match: XP_038902284.1 (uncharacterized membrane protein At3g27390 isoform X1 [Benincasa hispida])

HSP 1 Score: 1026.5 bits (2653), Expect = 9.5e-296
Identity = 527/585 (90.09%), Postives = 544/585 (92.99%), Query Frame = 0

Query: 55  MRVPVGFFPNLWSFVSFLPFFFFLLVLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPAHFF 114
           MRVPVGFFP LWSFVS LPFFF LL+LGFLKAVIVAPIAAAIVVIGNSSVIVGLFPAHFF
Sbjct: 1   MRVPVGFFPKLWSFVSLLPFFFLLLLLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPAHFF 60

Query: 115 WTFFCFARTKRLGIVLKTAVLVFLPLPLILWPVVGVVGSLLGGIGYGFFVPLIATFEAVG 174
           WT FC ARTKRLG+VLKT VLV LPLPL+LWPVVGVVGSLLGGIGYGFFVPLIATFEAVG
Sbjct: 61  WTLFCLARTKRLGLVLKTVVLVCLPLPLMLWPVVGVVGSLLGGIGYGFFVPLIATFEAVG 120

Query: 175 AGVTDKLYHCLADGCLSTTKASCLVVMDFTDFCFHSYFSYMDELGELMHSDEKPMEVKLS 234
           AGVTDKLYHCLADGCLST KASCLVVMDFTDFCFHSYFSYMDEL ELMHSDEKPMEVKLS
Sbjct: 121 AGVTDKLYHCLADGCLSTIKASCLVVMDFTDFCFHSYFSYMDELSELMHSDEKPMEVKLS 180

Query: 235 RLPCCLLASLIGVSVDLLFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG 294
           RLPCCLLASLIGV VD LFITLVAL RSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG
Sbjct: 181 RLPCCLLASLIGVPVDFLFITLVALCRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG 240

Query: 295 LAIILWPLAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSMFDEYVNDLL 354
           LAIILWPLAVVGAV SAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVS+FDEYVNDLL
Sbjct: 241 LAIILWPLAVVGAVTSAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSIFDEYVNDLL 300

Query: 355 YLREGSCIPRPKYRRNVNSDLKREHRSDDKNDGRSIRDGSNNHRLVSEQSRTLKWVIHQY 414
           YLREGSCIPRPKYRRN++SDL+REH  DDKND RSIRDGSNNH+LVSEQSRTLKW I  Y
Sbjct: 301 YLREGSCIPRPKYRRNMSSDLEREHCGDDKNDRRSIRDGSNNHKLVSEQSRTLKWAIQHY 360

Query: 415 KPVQVWDWLFKSCEVNGRILLQDGLISMEDIEECILKGNCKKLSIKLPAWCILQCLLSSA 474
           KP+QVWDWLFKSCEVNGRILLQ+GLI+ME+IEECILKGNCKKLSIKLPAWCILQCLLSSA
Sbjct: 361 KPIQVWDWLFKSCEVNGRILLQNGLITMENIEECILKGNCKKLSIKLPAWCILQCLLSSA 420

Query: 475 KSNSPGLIISDGVELTKTNLPRDTMFEWFLGPLLIMKEQIKRLHLEESEEICLRILIMRC 534
           KSNS GL+ISD VELT+TNLPRDTMFEWFLGPLLIMKEQIKRLHLEE+EE CLRILIMRC
Sbjct: 421 KSNSSGLLISDDVELTRTNLPRDTMFEWFLGPLLIMKEQIKRLHLEENEEKCLRILIMRC 480

Query: 535 RNEIPEDWDDFGFPSSDTVRRAQLQGIFRRLQGIVNSMSRIPTFRRRFRSLIKVLYVEAL 594
           RNEIPEDWDDFGFPS+D VRRAQLQ IFRRLQGIVNSMSRIPTFRRRFRSLIKVLYVE L
Sbjct: 481 RNEIPEDWDDFGFPSNDAVRRAQLQAIFRRLQGIVNSMSRIPTFRRRFRSLIKVLYVEGL 540

Query: 595 QMGSSANATRIRNGSKRLGRSRDGRNREEETANTIRKAPNNTDVV 640
           QMGSS    R  NGSKR G        EEETANTIRKAP+ TDVV
Sbjct: 541 QMGSSVKTMRTINGSKRFG--------EEETANTIRKAPSITDVV 577

BLAST of HG10000065 vs. NCBI nr
Match: XP_023512953.1 (uncharacterized membrane protein At3g27390 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1007.3 bits (2603), Expect = 6.0e-290
Identity = 510/584 (87.33%), Postives = 546/584 (93.49%), Query Frame = 0

Query: 55  MRVPVGFFPNLWSFVSFLPFFFFLLVLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPAHFF 114
           MRVPVGFFP LWSF+SFLPFFF LLVLG LKAVIV PIAAAIVV+GNSSVIVGLFPAHFF
Sbjct: 1   MRVPVGFFPKLWSFISFLPFFFLLLVLGLLKAVIVGPIAAAIVVVGNSSVIVGLFPAHFF 60

Query: 115 WTFFCFARTKRLGIVLKTAVLVFLPLPLILWPVVGVVGSLLGGIGYGFFVPLIATFEAVG 174
           WTF C ARTKRLG+VLK+ VLVFLPLPLILWP+VGV+GSLLGGIGYGFFVPLIATFEAVG
Sbjct: 61  WTFVCLARTKRLGLVLKSVVLVFLPLPLILWPIVGVIGSLLGGIGYGFFVPLIATFEAVG 120

Query: 175 AGVTDKLYHCLADGCLSTTKASCLVVMDFTDFCFHSYFSYMDELGELMHSDEKPMEVKLS 234
            G+TDKL+HC+ADGCLST KASC++VMDFTDFCFHSYFS+MDELGELM+SDEKP+EVKLS
Sbjct: 121 GGITDKLFHCVADGCLSTIKASCVIVMDFTDFCFHSYFSFMDELGELMYSDEKPIEVKLS 180

Query: 235 RLPCCLLASLIGVSVDLLFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG 294
           RLP CLLASLIGV VD L ITLVALWRSP+MLFKGWKR+LEDLVGREGPFLEAVCVPFAG
Sbjct: 181 RLPSCLLASLIGVLVDSLLITLVALWRSPFMLFKGWKRMLEDLVGREGPFLEAVCVPFAG 240

Query: 295 LAIILWPLAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSMFDEYVNDLL 354
           LAIILWP+AVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVL+VVSMFDEYVNDLL
Sbjct: 241 LAIILWPIAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLAVVSMFDEYVNDLL 300

Query: 355 YLREGSCIPRPKYRRNVNSDLKREHRSDDKNDGRSIRDGSNNHRLVSEQSRTLKWVIHQY 414
           YL EGSCIPRPKYRRN++SDLK EHRSDD ND RSIR GS+NH+LVSEQSRTLK  I QY
Sbjct: 301 YLGEGSCIPRPKYRRNMSSDLK-EHRSDDNNDQRSIRHGSSNHKLVSEQSRTLKRTIQQY 360

Query: 415 KPVQVWDWLFKSCEVNGRILLQDGLISMEDIEECILKGNCKKLSIKLPAWCILQCLLSSA 474
           KPVQ WDWLFKSCEVNGRILLQDGLISMED+EECILKGNCKKL++KLPAWCILQCLLSSA
Sbjct: 361 KPVQFWDWLFKSCEVNGRILLQDGLISMEDVEECILKGNCKKLTVKLPAWCILQCLLSSA 420

Query: 475 KSNSPGLIISDGVELTKTNLPRDTMFEWFLGPLLIMKEQIKRLHLEESEEICLRILIMRC 534
           KSNSPGLIISD VELT+TNLPRDTMFEWFLGPLLIMKEQIKRL+LEE+EE CLRILIMRC
Sbjct: 421 KSNSPGLIISDDVELTRTNLPRDTMFEWFLGPLLIMKEQIKRLNLEENEETCLRILIMRC 480

Query: 535 RNEIPEDWDDFGFPSSDTVRRAQLQGIFRRLQGIVNSMSRIPTFRRRFRSLIKVLYVEAL 594
           RNE PEDWD+F +PSSDTVRRAQLQ IFRRLQGIV+ MSRIP+FRRRF +LIKVLYVE +
Sbjct: 481 RNEKPEDWDEFAYPSSDTVRRAQLQAIFRRLQGIVSFMSRIPSFRRRFMNLIKVLYVEGI 540

Query: 595 QMGSSANATRIRNGSKRLGRSRDGRNREEETANTIRKAPNNTDV 639
           QMGSSA A R+ NGSKRLGRSRDGRNREEET NT +KA N  DV
Sbjct: 541 QMGSSATAARVGNGSKRLGRSRDGRNREEETINTAQKALNVLDV 583

BLAST of HG10000065 vs. NCBI nr
Match: XP_022987036.1 (uncharacterized membrane protein At3g27390 [Cucurbita maxima])

HSP 1 Score: 999.2 bits (2582), Expect = 1.6e-287
Identity = 503/584 (86.13%), Postives = 542/584 (92.81%), Query Frame = 0

Query: 55  MRVPVGFFPNLWSFVSFLPFFFFLLVLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPAHFF 114
           MRVPVGFFP LWSF+SFLPFFF LLVLG LKAVIV PIAAAIVV+GNS VIVGLFPAHFF
Sbjct: 1   MRVPVGFFPKLWSFISFLPFFFLLLVLGLLKAVIVGPIAAAIVVVGNSFVIVGLFPAHFF 60

Query: 115 WTFFCFARTKRLGIVLKTAVLVFLPLPLILWPVVGVVGSLLGGIGYGFFVPLIATFEAVG 174
           WTF C  RTKRLG+VLK+ VLVFLPLPLILWP+VGV+GSLLGGIGYGFFVPLIATFEAVG
Sbjct: 61  WTFVCLVRTKRLGLVLKSVVLVFLPLPLILWPIVGVIGSLLGGIGYGFFVPLIATFEAVG 120

Query: 175 AGVTDKLYHCLADGCLSTTKASCLVVMDFTDFCFHSYFSYMDELGELMHSDEKPMEVKLS 234
            G+TDKL+H +ADGCLST KASC++VMDFTDFCFHSYFS+MDELGELM+SDEKP+EVKLS
Sbjct: 121 GGITDKLFHSVADGCLSTIKASCVIVMDFTDFCFHSYFSFMDELGELMYSDEKPIEVKLS 180

Query: 235 RLPCCLLASLIGVSVDLLFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG 294
           RLP CLLASLIGV VD L ITL+ALWRSP+MLFKGWKR+LEDLVGREGPFLEAVCVPFAG
Sbjct: 181 RLPSCLLASLIGVLVDSLLITLIALWRSPFMLFKGWKRMLEDLVGREGPFLEAVCVPFAG 240

Query: 295 LAIILWPLAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSMFDEYVNDLL 354
           LAIILWP+AVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVL+VVSMFDEYVNDLL
Sbjct: 241 LAIILWPIAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLAVVSMFDEYVNDLL 300

Query: 355 YLREGSCIPRPKYRRNVNSDLKREHRSDDKNDGRSIRDGSNNHRLVSEQSRTLKWVIHQY 414
           YL EGSCIPRPKYRRN++SDLK+EH SDD ND RSIR GS+NH+LVSEQSRTLK  I QY
Sbjct: 301 YLGEGSCIPRPKYRRNMSSDLKKEHHSDDNNDQRSIRHGSSNHKLVSEQSRTLKQAIQQY 360

Query: 415 KPVQVWDWLFKSCEVNGRILLQDGLISMEDIEECILKGNCKKLSIKLPAWCILQCLLSSA 474
           KPVQ WDWLFKSCEVNGRILLQDGLISMED+EECILKGNCKKL++KLPAWCILQCLLSSA
Sbjct: 361 KPVQFWDWLFKSCEVNGRILLQDGLISMEDVEECILKGNCKKLTVKLPAWCILQCLLSSA 420

Query: 475 KSNSPGLIISDGVELTKTNLPRDTMFEWFLGPLLIMKEQIKRLHLEESEEICLRILIMRC 534
           KSNSPGLIISD VELT+TNLPRDTMF+W LGPLLIMKEQIKRL+LEE+EE CLRILIMRC
Sbjct: 421 KSNSPGLIISDDVELTRTNLPRDTMFDWCLGPLLIMKEQIKRLNLEENEETCLRILIMRC 480

Query: 535 RNEIPEDWDDFGFPSSDTVRRAQLQGIFRRLQGIVNSMSRIPTFRRRFRSLIKVLYVEAL 594
           RNE PEDWD+F +PSSDTVRRAQLQ IFRRLQGIV+ MSRIP+FRRRF +LIKVLYVE +
Sbjct: 481 RNEKPEDWDEFAYPSSDTVRRAQLQAIFRRLQGIVSFMSRIPSFRRRFMNLIKVLYVEGI 540

Query: 595 QMGSSANATRIRNGSKRLGRSRDGRNREEETANTIRKAPNNTDV 639
           QMGSSA A R+RNG KRLGRSRDGRNREEET NT +KA N  DV
Sbjct: 541 QMGSSATAARVRNGRKRLGRSRDGRNREEETINTAQKALNVLDV 584

BLAST of HG10000065 vs. ExPASy Swiss-Prot
Match: Q8GUM4 (Uncharacterized membrane protein At3g27390 OS=Arabidopsis thaliana OX=3702 GN=At3g27390 PE=1 SV=2)

HSP 1 Score: 440.7 bits (1132), Expect = 2.9e-122
Identity = 248/541 (45.84%), Postives = 337/541 (62.29%), Query Frame = 0

Query: 55  MRVPVGFFPNLWSFVSFLPFFFFLLVLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPAHFF 114
           M  P+GF  +L+ F+ FLP+F  LL LGF+K +++ P+   +V IGNS+VI+ L P H  
Sbjct: 1   MEPPIGFRASLFQFLLFLPYFIGLLFLGFIKGIVLCPLVCLVVTIGNSAVILSLLPVHIV 60

Query: 115 WTFFCFARTKRLGIVLKTAVLVFLPLPLILWPVVGVVGSLLGGIGYGFFVPLIATFEAVG 174
           WTF+     K++G +LK  + + LP  +ILWP+VG++GS+LGG  YGFF P+ ATF+AVG
Sbjct: 61  WTFYSIVSAKQVGPILKIFLCLCLPAAIILWPIVGILGSVLGGALYGFFSPIFATFDAVG 120

Query: 175 AGVTDKLYHCLADGCLSTTKASCLVVMDFTDFCFHSYFSYMDELGELMHSDEKPMEVKLS 234
            G   + +HC  DG  ST + S  VV DF D CFHSYFS MDEL +    D K  E++L 
Sbjct: 121 EGKPYQFFHCFYDGTWSTMQRSFTVVRDFKDVCFHSYFSLMDELKQSC-PDRKYYEIRLL 180

Query: 235 RLPCCLLASLIGVSVDLLFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG 294
           +LP  L+ S++G+ VD   I+LVA+ +SPYMLFKGW RL  DL+GREGPFLE +CVP AG
Sbjct: 181 QLPGALVVSVLGILVDPPVISLVAICKSPYMLFKGWHRLFHDLIGREGPFLETMCVPIAG 240

Query: 295 LAIILWPLAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSMFDEYVNDLL 354
           LAI+LWPLAV GAVI +V+SS FLG YAGV+ +QE SF  GL Y+++ VS++DEY  D+L
Sbjct: 241 LAILLWPLAVTGAVIGSVISSIFLGAYAGVVSYQESSFYYGLCYIVASVSIYDEYSTDIL 300

Query: 355 YLREGSCIPRPKYRRNVNSDLKRE---HRSDDKNDGRSIRDGSNNHRLVSEQSRTLKWVI 414
            L EGSC PRPKYRR             R     +  S+R GS    ++           
Sbjct: 301 DLPEGSCFPRPKYRRKDEEPTPFSGPVPRLGSVKNASSMRGGSVRVPMI----------- 360

Query: 415 HQYKPVQVWDWLFKSCEVNGRILLQDGLISMEDIEECILKGNCKKLSIKLPAWCILQCLL 474
              KP+ + + LF  C   G +L   GLI+ +DIEE       + +S+ LPA+ +L  +L
Sbjct: 361 -DIKPLDLLNELFVECRRYGEVLATKGLINSKDIEEARSSKGSQVISVGLPAYGLLYEIL 420

Query: 475 SSAKSNSPGLIISDGV-ELTKTNLPRDTMFEWFLGPLLIMKEQIKRLHLEESEEICL--R 534
            S K+NS GL++SDGV E+T  N P+D  F+WFL P LI+KEQ+K  +L E EE  L   
Sbjct: 421 RSVKANSSGLLLSDGVTEITTMNRPKDVFFDWFLNPFLILKEQMKATNLSEEEEEYLGRL 480

Query: 535 ILIMRCRNEIPEDWDDFGFPSSDTVRRAQLQGIFRRLQGIVNSMSRIPTFRRRFRSLIKV 590
           +L+      +         P     +RA+L    RR+QG+  ++SR PTFRR F +L+K 
Sbjct: 481 VLLFGDPERLKSSNAISASPPLTERKRAELDAFARRMQGLTKTVSRYPTFRRHFVALVKK 528

BLAST of HG10000065 vs. ExPASy TrEMBL
Match: A0A1S3BJL1 (uncharacterized membrane protein At3g27390 OS=Cucumis melo OX=3656 GN=LOC103490423 PE=4 SV=1)

HSP 1 Score: 1033.5 bits (2671), Expect = 3.8e-298
Identity = 520/588 (88.44%), Postives = 558/588 (94.90%), Query Frame = 0

Query: 52  ITLMRVPVGFFPNLWSFVSFLPFFFFLLVLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPA 111
           +TL+RVP GFFPNLW F+SFLPFFF LL+LGFLKAVIVAPI+AAI+VI NSSVIVGLFPA
Sbjct: 1   MTLVRVPFGFFPNLWRFISFLPFFFLLLLLGFLKAVIVAPISAAIIVICNSSVIVGLFPA 60

Query: 112 HFFWTFFCFARTKRLGIVLKTAVLVFLPLPLILWPVVGVVGSLLGGIGYGFFVPLIATFE 171
           HFFWTFFCFARTK+LGIV KT VLVFLPLPLILWP VG+VGSL+GGIGYGFFVPLIATFE
Sbjct: 61  HFFWTFFCFARTKKLGIVFKTVVLVFLPLPLILWPAVGIVGSLVGGIGYGFFVPLIATFE 120

Query: 172 AVGAGVTDKLYHCLADGCLSTTKASCLVVMDFTDFCFHSYFSYMDELGELMHSDEKPMEV 231
           AVGAGVTDKLYHCLADGCLST KASCLVVMDFTDFCF+SYFSYMDELGELM SDEKPMEV
Sbjct: 121 AVGAGVTDKLYHCLADGCLSTIKASCLVVMDFTDFCFNSYFSYMDELGELMLSDEKPMEV 180

Query: 232 KLSRLPCCLLASLIGVSVDLLFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVP 291
           KLSRLP CLLASLIG+ VD +FITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVP
Sbjct: 181 KLSRLPYCLLASLIGLPVDFIFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVP 240

Query: 292 FAGLAIILWPLAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSMFDEYVN 351
           FAGLAIILWPLAVVGAVISA++SSFFLGLYAGVIVHQEDSF+LGLAY+LSVVSMFDEYVN
Sbjct: 241 FAGLAIILWPLAVVGAVISAIISSFFLGLYAGVIVHQEDSFQLGLAYILSVVSMFDEYVN 300

Query: 352 DLLYLREGSCIPRPKYRRNVNSDLKREHRSDDKNDGRSIRDGSNNHRLVSEQSRTLKWVI 411
           DLLYLREGSCIPRPKYRRN++SDLK +HRSDDKND RS+ +G+NNH LVSEQSRTLKWVI
Sbjct: 301 DLLYLREGSCIPRPKYRRNMSSDLKMKHRSDDKNDARSMGNGANNHNLVSEQSRTLKWVI 360

Query: 412 HQYKPVQVWDWLFKSCEVNGRILLQDGLISMEDIEECILKGNCKKLSIKLPAWCILQCLL 471
             YKPV VWDWLF SCEVNGR+LLQDGLI+ EDIEECILKGN KKLSI+LPAWCILQCLL
Sbjct: 361 QHYKPVLVWDWLFMSCEVNGRLLLQDGLITTEDIEECILKGNSKKLSIRLPAWCILQCLL 420

Query: 472 SSAKSNSPGLIISDGVELTKTNLPRDTMFEWFLGPLLIMKEQIKRLHLEESEEICLRILI 531
           SSAKSNSPGL+IS+GVELT+TNLPRDTMFEWFLGPLL+MKEQIKRLHLEE+EEICLRILI
Sbjct: 421 SSAKSNSPGLVISNGVELTRTNLPRDTMFEWFLGPLLLMKEQIKRLHLEENEEICLRILI 480

Query: 532 MRCRNEIPEDWDDFGFPSSDTVRRAQLQGIFRRLQGIVNSMSRIPTFRRRFRSLIKVLYV 591
           MRCRNEIPEDWD+FGFPS D VRRAQLQ IFRRLQGIVNS+SRIP+FRRRFRSLIKVLYV
Sbjct: 481 MRCRNEIPEDWDNFGFPSLDRVRRAQLQAIFRRLQGIVNSVSRIPSFRRRFRSLIKVLYV 540

Query: 592 EALQMGSSANATRIRNGSKRLGRSRDGRNREEETANTIRKAPNNTDVV 640
           EALQ GSSA+ATRIRNGS+ LG  RDGRN EEETANT++KAPN+TDVV
Sbjct: 541 EALQKGSSADATRIRNGSEPLGNRRDGRNGEEETANTVQKAPNSTDVV 588

BLAST of HG10000065 vs. ExPASy TrEMBL
Match: A0A0A0KA80 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G113570 PE=4 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 1.0e-295
Identity = 518/596 (86.91%), Postives = 554/596 (92.95%), Query Frame = 0

Query: 55  MRVPVGFFPNLWSFVSFLPFFFFLLVLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPAHFF 114
           MRVP GFFP LWSF+SFLPFF  LL+LG LKA IVAPI+AAI+VI NSSVI+GLFPAHFF
Sbjct: 1   MRVPFGFFPYLWSFISFLPFFLLLLLLGLLKAAIVAPISAAIIVICNSSVILGLFPAHFF 60

Query: 115 WTFFCFARTKRLGIVLKTAVLVFLPLPLILWPVVGVVGSLLGGIGYGFFVPLIATFEAVG 174
           WTFFCFARTK+LGI+LKT VLVFLPLPLILWPVVG+VGSLLGGIGYGFFVPLIATFEAVG
Sbjct: 61  WTFFCFARTKKLGIILKTVVLVFLPLPLILWPVVGIVGSLLGGIGYGFFVPLIATFEAVG 120

Query: 175 AGVTDKLYHCLADGCLSTTKASCLVVMDFTDFCFHSYFSYMDELGELMHSDEKPMEVKLS 234
           AGVTDKLYHCLADGCLST KASCL+VMDFTDFCF+SYFSYMDELGELMHSDEKPMEVKLS
Sbjct: 121 AGVTDKLYHCLADGCLSTIKASCLIVMDFTDFCFNSYFSYMDELGELMHSDEKPMEVKLS 180

Query: 235 RLPCCLLASLIGVSVDLLFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG 294
           RLPCCLLASLIGV VD +FITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG
Sbjct: 181 RLPCCLLASLIGVPVDFIFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG 240

Query: 295 LAIILWPLAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSMFDEYVNDLL 354
           LAIILWPLAVVGAVISA++SS FLGLYAGVIVHQEDSF+LG+AYVLSVVSMFDEYVNDLL
Sbjct: 241 LAIILWPLAVVGAVISAIISSLFLGLYAGVIVHQEDSFQLGIAYVLSVVSMFDEYVNDLL 300

Query: 355 YLREGSCIP-----------RPKYRRNVNSDLKREHRSDDKNDGRSIRDGSNNHRLVSEQ 414
           YLREGSCIP           RPKYRRN +SDLKREHRSD+KND RS+++G NNH+LVSEQ
Sbjct: 301 YLREGSCIPRSFMHYIIVTARPKYRRNTSSDLKREHRSDNKNDARSMKNGENNHKLVSEQ 360

Query: 415 SRTLKWVIHQYKPVQVWDWLFKSCEVNGRILLQDGLISMEDIEECILKGNCKKLSIKLPA 474
           SRTLKWVI  YKPV VWDWLF SCEVNGR+LLQDGLI+ EDIEECILKGNCKKLSIKLPA
Sbjct: 361 SRTLKWVIQHYKPVLVWDWLFMSCEVNGRLLLQDGLITTEDIEECILKGNCKKLSIKLPA 420

Query: 475 WCILQCLLSSAKSNSPGLIISDGVELTKTNLPRDTMFEWFLGPLLIMKEQIKRLHLEESE 534
           WCILQCLLSSAKSNSPGL+IS+ VELT+TNLPRDTMFEWFLGPLL+MKEQIKRLHLEE+E
Sbjct: 421 WCILQCLLSSAKSNSPGLVISNNVELTRTNLPRDTMFEWFLGPLLLMKEQIKRLHLEENE 480

Query: 535 EICLRILIMRCRNEIPEDWDDFGFPSSDTVRRAQLQGIFRRLQGIVNSMSRIPTFRRRFR 594
           EICLRILIM+CRNE PEDWD+FGFPS++TVRRAQLQ IFRRLQGIVNS+SRIP+FRRRFR
Sbjct: 481 EICLRILIMKCRNEKPEDWDNFGFPSNNTVRRAQLQAIFRRLQGIVNSVSRIPSFRRRFR 540

Query: 595 SLIKVLYVEALQMGSSANATRIRNGSKRLGRSRDGRNREEETANTIRKAPNNTDVV 640
           SLIKVLYVEALQ GSSA+ATRIRNGSK LG  RDGRN EEETANTI+K PN TD V
Sbjct: 541 SLIKVLYVEALQKGSSADATRIRNGSKPLGNLRDGRNGEEETANTIQKPPNTTDDV 596

BLAST of HG10000065 vs. ExPASy TrEMBL
Match: A0A6J1JCZ6 (uncharacterized membrane protein At3g27390 OS=Cucurbita maxima OX=3661 GN=LOC111484600 PE=4 SV=1)

HSP 1 Score: 999.2 bits (2582), Expect = 7.9e-288
Identity = 503/584 (86.13%), Postives = 542/584 (92.81%), Query Frame = 0

Query: 55  MRVPVGFFPNLWSFVSFLPFFFFLLVLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPAHFF 114
           MRVPVGFFP LWSF+SFLPFFF LLVLG LKAVIV PIAAAIVV+GNS VIVGLFPAHFF
Sbjct: 1   MRVPVGFFPKLWSFISFLPFFFLLLVLGLLKAVIVGPIAAAIVVVGNSFVIVGLFPAHFF 60

Query: 115 WTFFCFARTKRLGIVLKTAVLVFLPLPLILWPVVGVVGSLLGGIGYGFFVPLIATFEAVG 174
           WTF C  RTKRLG+VLK+ VLVFLPLPLILWP+VGV+GSLLGGIGYGFFVPLIATFEAVG
Sbjct: 61  WTFVCLVRTKRLGLVLKSVVLVFLPLPLILWPIVGVIGSLLGGIGYGFFVPLIATFEAVG 120

Query: 175 AGVTDKLYHCLADGCLSTTKASCLVVMDFTDFCFHSYFSYMDELGELMHSDEKPMEVKLS 234
            G+TDKL+H +ADGCLST KASC++VMDFTDFCFHSYFS+MDELGELM+SDEKP+EVKLS
Sbjct: 121 GGITDKLFHSVADGCLSTIKASCVIVMDFTDFCFHSYFSFMDELGELMYSDEKPIEVKLS 180

Query: 235 RLPCCLLASLIGVSVDLLFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG 294
           RLP CLLASLIGV VD L ITL+ALWRSP+MLFKGWKR+LEDLVGREGPFLEAVCVPFAG
Sbjct: 181 RLPSCLLASLIGVLVDSLLITLIALWRSPFMLFKGWKRMLEDLVGREGPFLEAVCVPFAG 240

Query: 295 LAIILWPLAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSMFDEYVNDLL 354
           LAIILWP+AVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVL+VVSMFDEYVNDLL
Sbjct: 241 LAIILWPIAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLAVVSMFDEYVNDLL 300

Query: 355 YLREGSCIPRPKYRRNVNSDLKREHRSDDKNDGRSIRDGSNNHRLVSEQSRTLKWVIHQY 414
           YL EGSCIPRPKYRRN++SDLK+EH SDD ND RSIR GS+NH+LVSEQSRTLK  I QY
Sbjct: 301 YLGEGSCIPRPKYRRNMSSDLKKEHHSDDNNDQRSIRHGSSNHKLVSEQSRTLKQAIQQY 360

Query: 415 KPVQVWDWLFKSCEVNGRILLQDGLISMEDIEECILKGNCKKLSIKLPAWCILQCLLSSA 474
           KPVQ WDWLFKSCEVNGRILLQDGLISMED+EECILKGNCKKL++KLPAWCILQCLLSSA
Sbjct: 361 KPVQFWDWLFKSCEVNGRILLQDGLISMEDVEECILKGNCKKLTVKLPAWCILQCLLSSA 420

Query: 475 KSNSPGLIISDGVELTKTNLPRDTMFEWFLGPLLIMKEQIKRLHLEESEEICLRILIMRC 534
           KSNSPGLIISD VELT+TNLPRDTMF+W LGPLLIMKEQIKRL+LEE+EE CLRILIMRC
Sbjct: 421 KSNSPGLIISDDVELTRTNLPRDTMFDWCLGPLLIMKEQIKRLNLEENEETCLRILIMRC 480

Query: 535 RNEIPEDWDDFGFPSSDTVRRAQLQGIFRRLQGIVNSMSRIPTFRRRFRSLIKVLYVEAL 594
           RNE PEDWD+F +PSSDTVRRAQLQ IFRRLQGIV+ MSRIP+FRRRF +LIKVLYVE +
Sbjct: 481 RNEKPEDWDEFAYPSSDTVRRAQLQAIFRRLQGIVSFMSRIPSFRRRFMNLIKVLYVEGI 540

Query: 595 QMGSSANATRIRNGSKRLGRSRDGRNREEETANTIRKAPNNTDV 639
           QMGSSA A R+RNG KRLGRSRDGRNREEET NT +KA N  DV
Sbjct: 541 QMGSSATAARVRNGRKRLGRSRDGRNREEETINTAQKALNVLDV 584

BLAST of HG10000065 vs. ExPASy TrEMBL
Match: A0A6J1FYT4 (uncharacterized membrane protein At3g27390 OS=Cucurbita moschata OX=3662 GN=LOC111449067 PE=4 SV=1)

HSP 1 Score: 996.9 bits (2576), Expect = 3.9e-287
Identity = 505/584 (86.47%), Postives = 542/584 (92.81%), Query Frame = 0

Query: 55  MRVPVGFFPNLWSFVSFLPFFFFLLVLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPAHFF 114
           MRVPVGFFP LWSF+SFLPFFF LLVLG LKAVIV PIAAAIVV+GNSSVIVGLFPAHFF
Sbjct: 1   MRVPVGFFPKLWSFISFLPFFFLLLVLGLLKAVIVGPIAAAIVVVGNSSVIVGLFPAHFF 60

Query: 115 WTFFCFARTKRLGIVLKTAVLVFLPLPLILWPVVGVVGSLLGGIGYGFFVPLIATFEAVG 174
           WTF C ARTKRLG+VLK+ VLVFLPLPLILWP+VGV+GSLLGGIGYGFFVPLIATFEAVG
Sbjct: 61  WTFVCLARTKRLGLVLKSVVLVFLPLPLILWPIVGVIGSLLGGIGYGFFVPLIATFEAVG 120

Query: 175 AGVTDKLYHCLADGCLSTTKASCLVVMDFTDFCFHSYFSYMDELGELMHSDEKPMEVKLS 234
            G+T KL+HC+ADGCLST KASC++VMDFTDFCFHSYFS+MDELGELM+SDEKP+EVKLS
Sbjct: 121 GGITGKLFHCVADGCLSTIKASCVIVMDFTDFCFHSYFSFMDELGELMYSDEKPIEVKLS 180

Query: 235 RLPCCLLASLIGVSVDLLFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG 294
           RLP CLLASLIGV VD L ITLVALWRSP+MLFKGWKR+LEDL+GREGPFLEAVCVPFAG
Sbjct: 181 RLPSCLLASLIGVLVDSLLITLVALWRSPFMLFKGWKRMLEDLIGREGPFLEAVCVPFAG 240

Query: 295 LAIILWPLAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSMFDEYVNDLL 354
           LAIILWP+AVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVL+VVSMFDEYVNDLL
Sbjct: 241 LAIILWPIAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLAVVSMFDEYVNDLL 300

Query: 355 YLREGSCIPRPKYRRNVNSDLKREHRSDDKNDGRSIRDGSNNHRLVSEQSRTLKWVIHQY 414
           YL EGSCIPRPKYRRN++SDLK EHRSDD ND RSIR GS+NH+LVSEQSRTLK  I QY
Sbjct: 301 YLGEGSCIPRPKYRRNMSSDLK-EHRSDDNNDQRSIRHGSSNHKLVSEQSRTLKRAIQQY 360

Query: 415 KPVQVWDWLFKSCEVNGRILLQDGLISMEDIEECILKGNCKKLSIKLPAWCILQCLLSSA 474
           KPVQ WDWLFKSCEVNGRILL DGLISMED+EECILKGNCKKL++KLPAWCILQCLLSSA
Sbjct: 361 KPVQFWDWLFKSCEVNGRILLHDGLISMEDVEECILKGNCKKLTVKLPAWCILQCLLSSA 420

Query: 475 KSNSPGLIISDGVELTKTNLPRDTMFEWFLGPLLIMKEQIKRLHLEESEEICLRILIMRC 534
           KSNSPGLIISD VELT+TNLPRDTMFEWFLGPLLIMKEQIKRL+LEE+EE CLRILIMRC
Sbjct: 421 KSNSPGLIISDDVELTRTNLPRDTMFEWFLGPLLIMKEQIKRLNLEENEETCLRILIMRC 480

Query: 535 RNEIPEDWDDFGFPSSDTVRRAQLQGIFRRLQGIVNSMSRIPTFRRRFRSLIKVLYVEAL 594
           RNE PEDWD+F +PSSDTVRRAQLQ IFRRLQGIV+ MSRIP+FRRRF +LIKVLYVE +
Sbjct: 481 RNEKPEDWDEFAYPSSDTVRRAQLQAIFRRLQGIVSFMSRIPSFRRRFMNLIKVLYVEGI 540

Query: 595 QMGSSANATRIRNGSKRLGRSRDGRNREEETANTIRKAPNNTDV 639
           QMGSSA A R+ NGSKRLGRS  GRNREEET NT +KA N  DV
Sbjct: 541 QMGSSATAARVGNGSKRLGRSGGGRNREEETINTAQKALNVLDV 583

BLAST of HG10000065 vs. ExPASy TrEMBL
Match: A0A5D3BP50 (Putative membrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold264G001240 PE=4 SV=1)

HSP 1 Score: 980.3 bits (2533), Expect = 3.8e-282
Identity = 492/556 (88.49%), Postives = 528/556 (94.96%), Query Frame = 0

Query: 84  LKAVIVAPIAAAIVVIGNSSVIVGLFPAHFFWTFFCFARTKRLGIVLKTAVLVFLPLPLI 143
           + +VIVAPI+AAI+VI NSSVIVGLFPAHFFWTFFCFARTK+LGIV KT VLVFLPLPLI
Sbjct: 46  ISSVIVAPISAAIIVICNSSVIVGLFPAHFFWTFFCFARTKKLGIVFKTVVLVFLPLPLI 105

Query: 144 LWPVVGVVGSLLGGIGYGFFVPLIATFEAVGAGVTDKLYHCLADGCLSTTKASCLVVMDF 203
           LWP VG+VGSL+GGIGYGFFVPLIATFEAVGAGVTDKLYHCLADGCLST KASCLVVMDF
Sbjct: 106 LWPAVGIVGSLVGGIGYGFFVPLIATFEAVGAGVTDKLYHCLADGCLSTIKASCLVVMDF 165

Query: 204 TDFCFHSYFSYMDELGELMHSDEKPMEVKLSRLPCCLLASLIGVSVDLLFITLVALWRSP 263
           TDFCF+SYFSYMDELGELM SDEKPMEVKLSRLP CLLASLIG+ VD +FITLVALWRSP
Sbjct: 166 TDFCFNSYFSYMDELGELMLSDEKPMEVKLSRLPYCLLASLIGLPVDFIFITLVALWRSP 225

Query: 264 YMLFKGWKRLLEDLVGREGPFLEAVCVPFAGLAIILWPLAVVGAVISAVVSSFFLGLYAG 323
           YMLFKGWKRLLEDLVGREGPFLEAVCVPFAGLAIILWPLAVVGAVISA++SSFFLGLYAG
Sbjct: 226 YMLFKGWKRLLEDLVGREGPFLEAVCVPFAGLAIILWPLAVVGAVISAIISSFFLGLYAG 285

Query: 324 VIVHQEDSFRLGLAYVLSVVSMFDEYVNDLLYLREGSCIPRPKYRRNVNSDLKREHRSDD 383
           VIVHQEDSF+LGLAY+LSVVSMFDEYVNDLLYLREGSCIPRPKYRRN++SDLK +HRSDD
Sbjct: 286 VIVHQEDSFQLGLAYILSVVSMFDEYVNDLLYLREGSCIPRPKYRRNMSSDLKMKHRSDD 345

Query: 384 KNDGRSIRDGSNNHRLVSEQSRTLKWVIHQYKPVQVWDWLFKSCEVNGRILLQDGLISME 443
           KND RS+ +G+NNH LVSEQSRTLKWVI  YKPV VWDWLF SCEVNGR+LLQDGLI+ E
Sbjct: 346 KNDARSMGNGANNHNLVSEQSRTLKWVIQHYKPVLVWDWLFMSCEVNGRLLLQDGLITTE 405

Query: 444 DIEECILKGNCKKLSIKLPAWCILQCLLSSAKSNSPGLIISDGVELTKTNLPRDTMFEWF 503
           DIEECILKGN KKLSI+LPAWCILQCLLSSAKSNSPGL+IS+GVELT+TNLPRDTMFEWF
Sbjct: 406 DIEECILKGNSKKLSIRLPAWCILQCLLSSAKSNSPGLVISNGVELTRTNLPRDTMFEWF 465

Query: 504 LGPLLIMKEQIKRLHLEESEEICLRILIMRCRNEIPEDWDDFGFPSSDTVRRAQLQGIFR 563
           LGPLL+MKEQIKRLHLEE+EEICLRILIMRCRNEIPEDWD+FGFPS D VRRAQLQ IFR
Sbjct: 466 LGPLLLMKEQIKRLHLEENEEICLRILIMRCRNEIPEDWDNFGFPSLDRVRRAQLQAIFR 525

Query: 564 RLQGIVNSMSRIPTFRRRFRSLIKVLYVEALQMGSSANATRIRNGSKRLGRSRDGRNREE 623
           RLQGIVNS+SRIP+FRRRFRSLIKVLYVEALQ GSSA+ATRIRNGS+ LG  RDGRN EE
Sbjct: 526 RLQGIVNSVSRIPSFRRRFRSLIKVLYVEALQKGSSADATRIRNGSEPLGNRRDGRNGEE 585

Query: 624 ETANTIRKAPNNTDVV 640
           ETANT++KAPN+TDVV
Sbjct: 586 ETANTVQKAPNSTDVV 601

BLAST of HG10000065 vs. TAIR 10
Match: AT4G12680.1 (unknown protein; INVOLVED IN: vegetative to reproductive phase transition of meristem; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G40640.1); Has 103 Blast hits to 103 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 103; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 773.9 bits (1997), Expect = 1.0e-223
Identity = 385/548 (70.26%), Postives = 455/548 (83.03%), Query Frame = 0

Query: 55  MRVPVGFFPNLWSFVSFLPFFFFLLVLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPAHFF 114
           M VP GFF  LWSFVSFLP+FF LL+LG  KA+I+ PI++AI+++GNS VI+GL+PAHF 
Sbjct: 1   MEVPKGFFEKLWSFVSFLPYFFLLLLLGVTKALIIGPISSAIILVGNSCVIIGLWPAHFI 60

Query: 115 WTFFCFARTKRLGIVLKTAVLVFLPLPLILWPVVGVVGSLLGGIGYGFFVPLIATFEAVG 174
           WT++C ARTKR+G+VLKT  LV  PLPL+LWPV G+VGSL GGI YGFF PL+ATFEAVG
Sbjct: 61  WTYYCLARTKRIGLVLKTLALVLFPLPLLLWPVAGIVGSLFGGIAYGFFTPLMATFEAVG 120

Query: 175 AGVTDKLYHCLADGCLSTTKASCLVVMDFTDFCFHSYFSYMDELGELMHSDEKPMEVKLS 234
             VT K YHC  DG  ST K SC VV DFTDFCFHSYFSYMDEL E++ +D +P+E+KLS
Sbjct: 121 ESVTSKCYHCFVDGSFSTIKGSCTVVTDFTDFCFHSYFSYMDELREMVSADVEPLEIKLS 180

Query: 235 RLPCCLLASLIGVSVDLLFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG 294
           RLP CLLASLIGV VD+L IT VA+++SPYML KGWKRLLEDLVGREGPFLE+VCVPFAG
Sbjct: 181 RLPSCLLASLIGVMVDVLLITAVAVYKSPYMLLKGWKRLLEDLVGREGPFLESVCVPFAG 240

Query: 295 LAIILWPLAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSMFDEYVNDLL 354
           LAI+LWPLAV GAVI++V+SSFFLGLY+GVIVHQEDSFR+GL Y+++ VS+FDEYVNDLL
Sbjct: 241 LAILLWPLAVAGAVIASVLSSFFLGLYSGVIVHQEDSFRMGLNYIIAAVSLFDEYVNDLL 300

Query: 355 YLREGSCIPRPKYRRNVNSDLKREHRSDDKN-DGRSIRDGSNNHRLVSEQSRTLKWVIHQ 414
           YLREG+ +PRP YR    +   +    + KN D +S R  S   +LVSEQSRTLK  I  
Sbjct: 301 YLREGTSLPRPCYRTKTETVHGKRILGESKNVDLKSKRSSSLGSKLVSEQSRTLKKAITL 360

Query: 415 YKPVQVWDWLFKSCEVNGRILLQDGLISMEDIEECILKGNCKKLSIKLPAWCILQCLLSS 474
           YKPVQVW+WLFKSCEVNGRILL+DGLI ++D+EEC++KGN KKL IKLPAW +LQCLL+S
Sbjct: 361 YKPVQVWEWLFKSCEVNGRILLRDGLIDVKDVEECLVKGNSKKLYIKLPAWTVLQCLLAS 420

Query: 475 AKSNSPGLIISDGVELTKTNLPRDTMFEWFLGPLLIMKEQIKRLHLEESEEICLRILIMR 534
           AKSNS GL+I+DGVELT+ N PRD +F W +GPLLIMKEQIK L L E EE CLR L+M 
Sbjct: 421 AKSNSSGLVITDGVELTELNSPRDKVFVWLVGPLLIMKEQIKNLKLTEDEEFCLRKLVMV 480

Query: 535 CRNEIPEDWDDFGFPSSDTVRRAQLQGIFRRLQGIVNSMSRIPTFRRRFRSLIKVLYVEA 594
           C+NE  EDWD+ GFPSSDTVR+AQLQ I RRLQG+V SMSRIPTFRRRF +L+KVLY+EA
Sbjct: 481 CKNERTEDWDNTGFPSSDTVRKAQLQAIIRRLQGMVASMSRIPTFRRRFMNLVKVLYIEA 540

Query: 595 LQMGSSAN 602
           L+MG+S N
Sbjct: 541 LEMGASGN 548

BLAST of HG10000065 vs. TAIR 10
Match: AT3G27390.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G40640.1); Has 101 Blast hits to 99 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 101; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 440.7 bits (1132), Expect = 2.1e-123
Identity = 248/541 (45.84%), Postives = 337/541 (62.29%), Query Frame = 0

Query: 55  MRVPVGFFPNLWSFVSFLPFFFFLLVLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPAHFF 114
           M  P+GF  +L+ F+ FLP+F  LL LGF+K +++ P+   +V IGNS+VI+ L P H  
Sbjct: 1   MEPPIGFRASLFQFLLFLPYFIGLLFLGFIKGIVLCPLVCLVVTIGNSAVILSLLPVHIV 60

Query: 115 WTFFCFARTKRLGIVLKTAVLVFLPLPLILWPVVGVVGSLLGGIGYGFFVPLIATFEAVG 174
           WTF+     K++G +LK  + + LP  +ILWP+VG++GS+LGG  YGFF P+ ATF+AVG
Sbjct: 61  WTFYSIVSAKQVGPILKIFLCLCLPAAIILWPIVGILGSVLGGALYGFFSPIFATFDAVG 120

Query: 175 AGVTDKLYHCLADGCLSTTKASCLVVMDFTDFCFHSYFSYMDELGELMHSDEKPMEVKLS 234
            G   + +HC  DG  ST + S  VV DF D CFHSYFS MDEL +    D K  E++L 
Sbjct: 121 EGKPYQFFHCFYDGTWSTMQRSFTVVRDFKDVCFHSYFSLMDELKQSC-PDRKYYEIRLL 180

Query: 235 RLPCCLLASLIGVSVDLLFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG 294
           +LP  L+ S++G+ VD   I+LVA+ +SPYMLFKGW RL  DL+GREGPFLE +CVP AG
Sbjct: 181 QLPGALVVSVLGILVDPPVISLVAICKSPYMLFKGWHRLFHDLIGREGPFLETMCVPIAG 240

Query: 295 LAIILWPLAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSMFDEYVNDLL 354
           LAI+LWPLAV GAVI +V+SS FLG YAGV+ +QE SF  GL Y+++ VS++DEY  D+L
Sbjct: 241 LAILLWPLAVTGAVIGSVISSIFLGAYAGVVSYQESSFYYGLCYIVASVSIYDEYSTDIL 300

Query: 355 YLREGSCIPRPKYRRNVNSDLKRE---HRSDDKNDGRSIRDGSNNHRLVSEQSRTLKWVI 414
            L EGSC PRPKYRR             R     +  S+R GS    ++           
Sbjct: 301 DLPEGSCFPRPKYRRKDEEPTPFSGPVPRLGSVKNASSMRGGSVRVPMI----------- 360

Query: 415 HQYKPVQVWDWLFKSCEVNGRILLQDGLISMEDIEECILKGNCKKLSIKLPAWCILQCLL 474
              KP+ + + LF  C   G +L   GLI+ +DIEE       + +S+ LPA+ +L  +L
Sbjct: 361 -DIKPLDLLNELFVECRRYGEVLATKGLINSKDIEEARSSKGSQVISVGLPAYGLLYEIL 420

Query: 475 SSAKSNSPGLIISDGV-ELTKTNLPRDTMFEWFLGPLLIMKEQIKRLHLEESEEICL--R 534
            S K+NS GL++SDGV E+T  N P+D  F+WFL P LI+KEQ+K  +L E EE  L   
Sbjct: 421 RSVKANSSGLLLSDGVTEITTMNRPKDVFFDWFLNPFLILKEQMKATNLSEEEEEYLGRL 480

Query: 535 ILIMRCRNEIPEDWDDFGFPSSDTVRRAQLQGIFRRLQGIVNSMSRIPTFRRRFRSLIKV 590
           +L+      +         P     +RA+L    RR+QG+  ++SR PTFRR F +L+K 
Sbjct: 481 VLLFGDPERLKSSNAISASPPLTERKRAELDAFARRMQGLTKTVSRYPTFRRHFVALVKK 528

BLAST of HG10000065 vs. TAIR 10
Match: AT5G40640.1 (unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 19 plant structures; EXPRESSED DURING: 7 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G27390.1); Has 104 Blast hits to 102 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 2; Fungi - 0; Plants - 101; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 435.3 bits (1118), Expect = 8.7e-122
Identity = 244/542 (45.02%), Postives = 341/542 (62.92%), Query Frame = 0

Query: 55  MRVPVGFFPNLWSFVSFLPFFFFLLVLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPAHFF 114
           M  P G   +LW F+ F+P+F  LL+LG +K +++ P+    V IGNS++I+GL P H  
Sbjct: 1   MEPPTGILASLWQFILFIPYFTGLLLLGVIKGIVLCPLICLTVAIGNSAIILGLLPVHAI 60

Query: 115 WTFFCFARTKRLGIVLKTAVLVFLPLPLILWPVVGVVGSLLGGIGYGFFVPLIATFEAVG 174
           WT +  A  K+LG +LK  + + +PL +ILW VV ++GS+LGG  YGF  P+ ATF+AVG
Sbjct: 61  WTLYSIASAKQLGPILKIFLCLCVPLGVILWLVVSILGSVLGGAIYGFLSPIFATFDAVG 120

Query: 175 AGVTDKLYHCLADGCLSTTKASCLVVMDFTDFCFHSYFSYMDELGELMHSDEKPMEVKLS 234
            G ++  +HC  DG  ST + S  VV DF D CFHSYFS+MD+L     ++    E++L 
Sbjct: 121 EGKSNPFFHCFYDGTWSTVQGSFTVVCDFKDVCFHSYFSFMDDL-RTSTANRHYYEIRLL 180

Query: 235 RLPCCLLASLIGVSVDLLFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAG 294
           ++P  ++ +++G+ VD   I+L+AL +SPYMLFKGW RL  DL+GREGPFLE +CVP AG
Sbjct: 181 QIPGAVIVAVLGILVDFPVISLLALCKSPYMLFKGWHRLFHDLIGREGPFLETMCVPIAG 240

Query: 295 LAIILWPLAVVGAVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSMFDEYVNDLL 354
           L I+LWPLAVVGAV+ +VVSS FLG Y GV+ +QE SF  GL YV++ VS++DEY ND+L
Sbjct: 241 LVILLWPLAVVGAVLGSVVSSVFLGAYGGVVSYQESSFFFGLCYVVASVSIYDEYSNDVL 300

Query: 355 YLREGSCIPRPKYRRNVNSDLKREHRSDDKNDGRSIRDGSNNHRLVSEQSRTLKWVIHQY 414
            + EGSC PRP YRRN       E  S   + G S     N+ +    +  + K  +   
Sbjct: 301 DMPEGSCFPRPIYRRN------EEGASTAFSGGLS---RPNSFKTTPSRGGSNKGPMIDL 360

Query: 415 KPVQVWDWLFKSCEVNGRILLQDGLISMEDIEECILKGNCKKLSIKLPAWCILQCLLSSA 474
           KP+ + + LF  C  +G I++  G+I+ +DIEE       + +S  LPA+ +L  LL S 
Sbjct: 361 KPLDLLEALFVECRRHGEIMVTKGIINSKDIEEAKSSKGSQVISFGLPAYSLLHELLRSI 420

Query: 475 KSNSPGLIISDGV-ELTKTNLPRDTMFEWFLGPLLIMKEQIKRLHLEESEEICLRILIM- 534
           KSNS GL++ DGV E+T  N P+D  F+WFL P LI+K+QI+  +L E EE  L  L++ 
Sbjct: 421 KSNSTGLLLGDGVTEITTRNRPKDAFFDWFLNPFLILKDQIEAANLSEEEEEYLGKLVLL 480

Query: 535 -----RCRNEIPEDWDDFGFPSSDTVRRAQLQGIFRRLQGIVNSMSRIPTFRRRFRSLIK 590
                R ++ I E       P    +R+A+L    RRLQG+  S+SR PTFRR F  L+K
Sbjct: 481 FGDSERLKSSIVESES----PPLTELRKAELDAFARRLQGLTKSVSRYPTFRRHFVELVK 528

BLAST of HG10000065 vs. TAIR 10
Match: AT4G37030.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G12680.1); Has 101 Blast hits to 99 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 101; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 377.5 bits (968), Expect = 2.2e-104
Identity = 213/533 (39.96%), Postives = 324/533 (60.79%), Query Frame = 0

Query: 69  VSFLPF-FFFLLVLGFLKAVIVAPIAAAIVVIGNSSVIVGLFPAHFFWTFFCFARTKRLG 128
           +S++ F F     LG +K +IV PIA   +++GN  VI+ LFPAH  WT +  A+T R  
Sbjct: 14  ISYVIFAFCSAFFLGAIKGLIVGPIAGLTLIVGNVGVILCLFPAHVTWTIYAVAKTNRFD 73

Query: 129 IVLKTAVLVFLPLPLILWPVVGVVGSLLGGIGYGFFVPLIATFEAVGAGV-TDKLYHCLA 188
           I LK A+LV LP    +W  + +  S+L G+GYGFF P I+ FEA      ++K +HCL 
Sbjct: 74  IPLKVAILVALPALFGIWLGLSLAISVLVGVGYGFFTPWISAFEAFRQDTESNKFFHCLV 133

Query: 189 DGCLSTTKASCLVVMDFTDFCFHSYFSYMDELGELMHSDEKPMEVKLSRLPCCLLASLIG 248
           DG   T K SC+VV DF DFC+HSY  Y+ EL E   SDE    ++L  +P C++  ++G
Sbjct: 134 DGTWGTIKGSCIVVTDFADFCYHSYPLYLKELRESPVSDEL-QTLRLIHVPGCIIVGILG 193

Query: 249 VSVDLLFITLVALWRSPYMLFKGWKRLLEDLVGREGPFLEAVCVPFAGLAIILWPLAVVG 308
           + +D+   T +A+ +SPY+L KGW RL +D + REGPFLE  C+P AGL ++LWP+ V+G
Sbjct: 194 LVIDIPLFTAIAVIKSPYLLLKGWYRLAQDAINREGPFLEIACIPVAGLTVLLWPIVVIG 253

Query: 309 AVISAVVSSFFLGLYAGVIVHQEDSFRLGLAYVLSVVSMFDEYVNDLLYLREGSCIPRPK 368
            ++  + SS F+GLY  V+V QE SFR G++YV++VV  FDEY ND LYLREG+  P+P+
Sbjct: 254 FILVTIFSSIFVGLYGAVVVFQERSFRRGVSYVIAVVGEFDEYTNDWLYLREGTIFPKPR 313

Query: 369 YRR---NVNSDLKREHRSDDKNDGRSIRDGSNNHRLVSE--QSRTLKWVIHQYKPVQVWD 428
           YR    + +S++       D     S         LV     S +++  I + + VQ+W+
Sbjct: 314 YRMGRGSFSSEVSVIVHPSDVTRVNSSGSVDAPAMLVPSLVHSVSVREAIQEVRMVQIWE 373

Query: 429 WLFKSCEVNGRILLQDGLISMEDIEECILKG----NCKKLSIKLPAWCILQCLLSSAKSN 488
            +    E+ G+ LL   +++  D+ E  LKG        +++ LP++ +L  LLSS K+ 
Sbjct: 374 HMMGWFEMQGKELLDAEVLTPTDLYES-LKGRHGNESSIINVGLPSYALLHTLLSSIKAG 433

Query: 489 SPGLIISDGVELTKTNLPRDTMFEWFLGPLLIMKEQIKRLHLEESEEICL-RILIMRCRN 548
             G+++ DG E+T  N P+D   +W   P++++K+QI+ L L ESE   L ++++     
Sbjct: 434 VHGVLLLDGSEVTHLNRPQDKFLDWVFNPIMVLKDQIRALKLGESEVKYLEKVVLFGNHE 493

Query: 549 EIPEDWDDFGFPSSDTVRRAQLQGIFRRLQGIVNSMSRIPTFRRRFRSLIKVL 590
           +  E WD+   P  + +R AQ+QGI RR+ G+V S+S++PT+RRRFR ++K L
Sbjct: 494 QRMEAWDNHSNPPQENLRTAQIQGISRRMMGMVRSVSKLPTYRRRFRQVVKAL 544