Cucsat.G9697 (gene) Cucumber (B10) v3

Overview
NameCucsat.G9697
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Descriptionglycosylphosphatidylinositol anchor attachment 1 protein-like
Locationctg1673: 5762064 .. 5767974 (-)
RNA-Seq ExpressionCucsat.G9697
SyntenyCucsat.G9697
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAAGTCACGAGGGAAGCATGGGGAGGATTAATCACAAGTGGTAAAAAGTTTTCTTGGGTCTCATATGCAAATTTTCCAAACCAATAAACCGATGGTCTTTGCTTTACACTATCAACCGATGGTTTAACCGGTTTGGGATTACCGCCCGGTTCATATGTTGCCTTCTCCGTGTCCGTCTTATTGTTAACTGAGATCACCTCGCATTTCTTCATCTTCTTCTTCTTCTTTCACTTGGTTTCTTATTTTTGCAACAGATTTGGAGCAATCGGGCCACTGTGAAGATTGCCTAGTCCGATGGCAGAGACCGAGAAACTAAAACCGAAGGCGCGGCCGATTGTGCGTCTGGGGATTTTCCTAATTTCACACAGTATTTTCTTCAGGTATGCTGTTGTTTTTACATGGATTTGTTTCTCAGTTCGTATCAGAATCTAGTGATTGATTAATGTCCTTGTTCTTTTCTTTCCTTTTTTGCGTGTTTTCTAGCGTGGTTTGCTTCTCTGCAGGGGTTTTAGCTCTTCTACTACTACCAATGTTCGCTATGAACACCTACATTTCCGAGAACGCTCTCATGCCAGGTACCTTTGGGTTCTTGCTTAACCCATCAATTTCTATTTATATATATGTATATATATATATTAACTTATTATTCATCATTTTTATGCTTTCGTGATTGATCCACTGGTGGTTTGAATGATTTAAGTTAAATTACAAATTAAGATTCATATGTTGTTGTTGCGAAGTATCTGAAATTATAGGAACATCAAGTTTATGTCTCTTTTTTTTTTTGCACATCTATTCATTTTACTTTTATTTGGATAGACATGACTCTTAACTGTGGTATTGTATTTTCCAATAGGGTCTGCAAATTCCATGCTTTCGGGTCGGGAAGTCTCAGAGGCGAACAATTTAGTAAAGGATCTGAAGGGCTTGAATTCAAAACCTGGCTCATCGATCTTGTAAGTACTTACTAGACTGGTTGCATCTATTTTATTTCTCCTTTAAAACGATGATGAAAACTTGTTTATGCTTAAGTTGTAATGCTTAAGATTTTGAGGATTACCACTTCACAGTGTAAAATTTTTCACGATTCATGTCTTGTATGCCCTGACATTAAAATGGTTTTATGCATATGCATGATTTAAAGGTAAGGCCTTGGAACTCAGTGAACTATTTGTCTAATTGCAAGAATTAAAGTCAATCAAGGGTAGGGTAAACTTCAACTTTCAAGTTCACTTATATACCATGGTTCGATTCGCTAAATATTTAAATATGTTATCCAACTTTAGCTATTTTGATGTGAATTTCTCAATGAGTTTAATGTTAGCGTCCTACATTTACAATCTTAAGAGGTTAGGAGAAACAGGCAAGCTGGGAAGAGTTATTGTTTGGATAGGTATTAAAAGACTAGTTCTAAAGTTCTCATATGAGCTAAATGTATTAGATGAATGCTTTTGCCAAAAAGTGGTATGGTTTATTGTTTTTATGATATGTGTGATGTTCTGCGTTAGTTTCATTTTATTTGACCATGTAGTCTAAGCGTGTGGATTGGCATGTTATGTTCATCAAGATAAATATGGGTGCATAGAGGTGATAAACTAAATTGTGCGACTCTTTTTGCTACTTGCAGTGTCTTCATAGTGTTAGATACCAAGATTTAGATAGGGCTAAGGGTAGTTAGTTATATAGGCAGTTAGATGGTTATAAATAGGAAGTTAGGGGGGGAAGAAAGGCATGAAGATTTTGGTGAAGATAATAGGCTGCAACATTCCTTGAAAGAAAGGAAGGGCAGATGGTTAGGGTTTCTTTAAGTGTTCTTGAGTTTCCTTTTACTTGTTTGTTCTTTTATATTGTAATTTCTGTTGAAGATATCAATAAACATAGACACTATATTGGTGTTCTATCACATAGTTATATAATTATCATTACCATCTTCTTCACATTTCCATAAGCATTATCTTCTTCTTCCCCTTCGCTCTGTTAGAGTTCCAAGTCTTTCTTTCTAGTCAACCAATGATAAATTAATTTGAGTTTCAATTCATTTTTCAGTGGAAGCCAGCAGATTTTGGCACAATATATTTCAAAATTGGGAGCAGATGTTAATTACCACAGGTTCCACCCGCAATCTAGTCAGTTCCATCCACTGCACTTTTTCTCTAGCCCTGATTCTGGTATCCTCAAGGACAATGTTAGTTGCATGACGCAGGGTATTAACACTGTTGGCATAATACGAGCACCCCAAGCAGATGGAAAGGAAGCCATTGTTTTGGTGACACCTTACAATCCTGTTGAGACCAGTCTTCATGATACTTTGTCTCTAGGTATTGCTTATTCAATATTTTCTTTGCTTACACAAGTAACTTGGTTGGCCAAAGACATTGTTTGGCTTGTTGCGGATTCAAGATATGGAGAATATGCTGCTGTTTCAGCTTGGCTTAGAGACTATCACACCCCTGTATTTGGTCAATCCAGCGTGATTGACACAGATGCTTGTTCTGAAACAAATGTCCTTGATGAATTTGAAGCCAACCAAGTGACAGAAAAAAGGATTCTAGATGATTTTAAACGTGCTGGAACGATGGCTGCAGCCCTTGTTATTAAGGTTTCAAATAGAAGCGAACACTTCGAGGACAGTCTTAGCGTCTATGCTGAAGCATCTAACGGTCAGATGCCGAACCTCGACCTCATCAATATTGTGAATTACCTAGCAGTACATAGGCAAGGTTTTCGGATTAAAATTGAGAAATTTTGGCCTTTACTAAACTGCAAATGGCTCAAGGTTTTGGGCGAAGTTTTTGAGTCAATAGGAAAGGTTATTAGAAGCTTGAACTCTGAGTGGAAATTTGGCATGTCAGCTTCTGACTATGTTGATGGCACAGCCACACTTGCAAGTTCGTTGTACTACCAGGTGGTTTACACTTCAGTTCATCTTTTAGGAGATTGTTATTTAGGGTTCATTTAATAAAAGAACGATGGATGTTTTCATTTATGTCTATAATTGGGATTATTTTCACTTAAATTATCCATGTCCTTGACATTAGGCTGTGGGTATTCCTACTGGTTCGCATGGGGCTTTTCGAGATTACCAAATTGATGCAATTACTGTGGAGATGTCCCCCAAATTTTCTTCTGGTATCAAGGTCAGGCGTGATGATTTCATACTGCGAGGTGGCCGGTAAGTGCTCATTTTTGCCTCATGCATTTGATAACGTGTCCTCCCACCCTCAACTTTTCAGGGTTCACTACGTGAATAAATGTTTAAAACCCACGATCTCCTGAATGGGGTTAGATCAACATCAAGTAAACTAATATTTATCTACTTTTAAATTATACATTATTTTCCATTTTTATCACGTGTTTCACTGTCAATCTTCAATGTTGAAGTTCTAATTTGTAAATTGTTTTATTGGGAATAATATAAAGTACTTTTTGTTCCCTAAAACGTACTTCGATTGGCTGTTGGGTTCTCCTTTTAAATGAATAGAATATTTTTCTATTCTTGTTATTCATTCAAGTGAATTTGTTGCCTAAATTCATGTTCACATATGGAAATGAGTTAGATTTTGATGCATTGAATTATCGCCTGGATTTTTTTGTATTAGTGAACTAGATTTTCATGCAGGGAAATGAGCCTAAACTTTGCAACGAAATTCTCCTGATCCTTTTGATTTGGTTAAGGATTTTGTGGTTGGTTATGCAGGCTAATCGAAGGAGTTGTGCGGTCAGTAAACAACCTCCTGGAGAAGTTCCATCAGTCATTCTTTCTATACCTTATGGTATCCACCGGCAAATTTGTATCAGTTGGTGTATATATGATTGCCTTTGCTCTGCTTGTTGCTCCCCTACCCGCAGTTGCGGCCGCTCTCTATTCCTATGCCAATAACTTGAATCTAACCTCAGAAAAGGTCGAACCTCCAGCTCTTGCAAATCCTGACGATGAGCTTATCGTCTCTTTAAGATCATGGAAATGGCTTAATGCAGCAAAAAGAGTTTTTGTTGTTCATCTATGGGGGGCTATTGTTTCCTTACTTCCCTACTTCATCTGCCAAATACCTGGTTACAGTCCTACAGAGAACTCTATTATATGGGGGTTGCTTTCACTGCTCTCGCTATTAGTTTTGTCTGTGATATTAGGTTCTCCATTTAGTTCCACTAAATCTTACGAACAACGAATTCAAGAGTGGGCTTTCTTGAAAGCAATGACCACCTCAGCGGCCTTTATTGGTTTGTGCCTCATGTCAGTAATTAACTTTTCTACTGCAGAACTTGGAGCCTTTTTGGTTGTATCAATGTGCTTGTTGGCACATCCCTTGAAGCTTGATTTAGGGGCTGGTAACTTTAAAGCTCTTTCAAGGGCAGCCTGTAACCTTGTTCTGGGATTCATAGCTTTTCCACCTGTTACTTTCTTCTTGTTTAAAGGTGCTTTGCAAGGTTTCGACAATCTACATATCGGCGACTTCTGGAACTGGATGGAAACCCTCTGGGCATGGAACAGTGCTACTTTCCTCTACTTAGGTATGGTTCACCTTCCATGCTGGTTATTATGTACACAAATTTTACTTCATCCATGTTGAGTACCTTGTTTAGTTTTTTACATAATATTTGATTAAACCAACCAATTAGTAGATTTCTTAGCTTTACTAAATTTTGTTCCCTTTAGGCTGAACAGTTAATTTATTGAACATCATTTAGTCCCTCAGAAATAGGTTTCAACTGTATTGGAACAAGTTACACGTTCATCCCTTGTATGGTAGCTTCTGTTTGCAGGGCTTAAAGCCTACTTTGCGTTATTGTTGTTTATACTTGTCGGACTTATACAGTAAGCTGTTATCCTCTTGAAAACTGCTTTTAGCCATATTTCTTTCTCCAATTCTTGGTGTTTCTTGAATTTCTTTCTCCAATTAGATTATGGTAATATAGCGCACATATATAAAGGCAATGACAGAATTTGGCAAAAATTAGCCGGTTGAGATTTATTTTAATTTTTGTGAGTCCGTGACTTTGCTCATCCATAGTGTTTCATCTATCTAAAATGAAGACTAATGGTACGGATGAGTTTGGTTTTGTTTCAAGCAACCTGGTTTTGCTCCTCTTCTGACTTCAAGGGAATAACCATTTTCTTGTTTCATGTTTATGTTTCCAATTTCTTGCTGGTATATAAATTTGGAAACATTTCTTATAAATGATCAATGGCAATTGACTTAATTTTTCTTGAATATGGAGGTTTTGATTCCTATTTCTATAGTTGTAGTAAAAGGAATTGGTACTTTTAAGTGTAAAAATGAAAAAGTTATAATCGGATCAACGTTGCCATGGACGAATGGTTCCATTGCCTTCAGACAATGTTGGCCAGTGCATTTTCGTCAATTTTGTAGTCGTTCACACTGCCAAACGTGCTGTCACGTCTCACCCATGTGCCACTGTCAGACCTTTATGTGTCGTTGCACGTGTTGTTGCATGCATTGTCGTATTGGAGCTACACACACTGGTCCTTCCTCCTCAGATAGCGTCGACCATCCAACTTGTGTAACCCTCTTCTTCATCTGCGACCTATATTGCTCCCCATGTCTGCTTCATGTATTGCCACCTAATTTTGGACGGCCACCACTACTTTTGTCATCCAATTTGTATGCCTTGCCACCCACTGACCTAGGTTATCATCTCGATGTTAAGAATCCTCAAACTTCCTCTTTGAGATTGGCAAATCTTCGGCACATTCCAGATAACGTGCAAACTTCTTCAGGAATGTCTGATCAACAACTGGATGGGCTTTGACAACAAATAGCAGTTGGAACAAGGGAACAAGGCAAATGTTCACTGTCAAATACAAACTTCTTCAGGAATGTCTGATCAACAACTGGATGGGCTTTGACAACAAATAGCAGTTGGAACAAGGGAACAAGGCAGATGTTCACTGTCAAATACAAGGTGGATGGAACTCTTGAAAAATACGAGGCAAGGCTAGTGGCAAAGGGGTTTACT

Coding sequence (CDS)

ATGGCAGAGACCGAGAAACTAAAACCGAAGGCGCGGCCGATTGTGCGTCTGGGGATTTTCCTAATTTCACACAGTATTTTCTTCAGCGTGGTTTGCTTCTCTGCAGGGGTTTTAGCTCTTCTACTACTACCAATGTTCGCTATGAACACCTACATTTCCGAGAACGCTCTCATGCCAGGGTCTGCAAATTCCATGCTTTCGGGTCGGGAAGTCTCAGAGGCGAACAATTTAGTAAAGGATCTGAAGGGCTTGAATTCAAAACCTGGCTCATCGATCTTTGGAAGCCAGCAGATTTTGGCACAATATATTTCAAAATTGGGAGCAGATGTTAATTACCACAGGTTCCACCCGCAATCTAGTCAGTTCCATCCACTGCACTTTTTCTCTAGCCCTGATTCTGGTATCCTCAAGGACAATGTTAGTTGCATGACGCAGGGTATTAACACTGTTGGCATAATACGAGCACCCCAAGCAGATGGAAAGGAAGCCATTGTTTTGGTGACACCTTACAATCCTGTTGAGACCAGTCTTCATGATACTTTGTCTCTAGGTATTGCTTATTCAATATTTTCTTTGCTTACACAAGTAACTTGGTTGGCCAAAGACATTGTTTGGCTTGTTGCGGATTCAAGATATGGAGAATATGCTGCTGTTTCAGCTTGGCTTAGAGACTATCACACCCCTGTATTTGGTCAATCCAGCGTGATTGACACAGATGCTTGTTCTGAAACAAATGTCCTTGATGAATTTGAAGCCAACCAAGTGACAGAAAAAAGGATTCTAGATGATTTTAAACGTGCTGGAACGATGGCTGCAGCCCTTGTTATTAAGGTTTCAAATAGAAGCGAACACTTCGAGGACAGTCTTAGCGTCTATGCTGAAGCATCTAACGGTCAGATGCCGAACCTCGACCTCATCAATATTGTGAATTACCTAGCAGTACATAGGCAAGGTTTTCGGATTAAAATTGAGAAATTTTGGCCTTTACTAAACTGCAAATGGCTCAAGGTTTTGGGCGAAGTTTTTGAGTCAATAGGAAAGGTTATTAGAAGCTTGAACTCTGAGTGGAAATTTGGCATGTCAGCTTCTGACTATGTTGATGGCACAGCCACACTTGCAAGTTCGTTGTACTACCAGGCTGTGGGTATTCCTACTGGTTCGCATGGGGCTTTTCGAGATTACCAAATTGATGCAATTACTGTGGAGATGTCCCCCAAATTTTCTTCTGGTATCAAGGTCAGGCGTGATGATTTCATACTGCGAGGTGGCCGGCTAATCGAAGGAGTTGTGCGGTCAGTAAACAACCTCCTGGAGAAGTTCCATCAGTCATTCTTTCTATACCTTATGGTATCCACCGGCAAATTTGTATCAGTTGGTGTATATATGATTGCCTTTGCTCTGCTTGTTGCTCCCCTACCCGCAGTTGCGGCCGCTCTCTATTCCTATGCCAATAACTTGAATCTAACCTCAGAAAAGGTCGAACCTCCAGCTCTTGCAAATCCTGACGATGAGCTTATCGTCTCTTTAAGATCATGGAAATGGCTTAATGCAGCAAAAAGAGTTTTTGTTGTTCATCTATGGGGGGCTATTGTTTCCTTACTTCCCTACTTCATCTGCCAAATACCTGGTTACAGTCCTACAGAGAACTCTATTATATGGGGGTTGCTTTCACTGCTCTCGCTATTAGTTTTGTCTGTGATATTAGGTTCTCCATTTAGTTCCACTAAATCTTACGAACAACGAATTCAAGAGTGGGCTTTCTTGAAAGCAATGACCACCTCAGCGGCCTTTATTGGTTTGTGCCTCATGTCAGTAATTAACTTTTCTACTGCAGAACTTGGAGCCTTTTTGGTTGTATCAATGTGCTTGTTGGCACATCCCTTGAAGCTTGATTTAGGGGCTGGTAACTTTAAAGCTCTTTCAAGGGCAGCCTGTAACCTTGTTCTGGGATTCATAGCTTTTCCACCTGTTACTTTCTTCTTGTTTAAAGGTGCTTTGCAAGGTTTCGACAATCTACATATCGGCGACTTCTGGAACTGGATGGAAACCCTCTGGGCATGGAACAGTGCTACTTTCCTCTACTTAGGTATGGTTCACCTTCCATGCTGGTTATTATGTACACAAATTTTACTTCATCCATGTTGA

Protein sequence

MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPGSANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALYSYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGLCLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFFLFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGMVHLPCWLLCTQILLHPC
Homology
BLAST of Cucsat.G9697 vs. ExPASy Swiss-Prot
Match: Q9WTK3 (Glycosylphosphatidylinositol anchor attachment 1 protein OS=Mus musculus OX=10090 GN=Gpaa1 PE=1 SV=3)

HSP 1 Score: 111.3 bits (277), Expect = 4.5e-23
Identity = 152/632 (24.05%), Postives = 259/632 (40.98%), Query Frame = 0

Query: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60
           +++  + +  AR ++RL   L   S    +  F A     L  P     TY+SENA+   
Sbjct: 4   LSDPVRRRALARIVLRLNTPLCVLSYVAGIAWFLA-----LAFPPLTQRTYMSENAMGST 63

Query: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120
                  G +   A +  +D      KPG+       +   ++ +    V    +    S
Sbjct: 64  MVEEQFVGGD--RARSFARDFAAHRKKPGA-------LPVAWLERSMRSVGLEVYTQSFS 123

Query: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180
           +  P      PD    + +   M  G N  GI+RAP++   E++VL  P  P  T   ++
Sbjct: 124 RKLPF-----PD----ETHERYMVSGTNVYGILRAPRSASTESLVLTVPCGPDAT---NS 183

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYH-TPVFG-QSSVIDT 240
            ++G+  ++ +      + AKDI++LV D    +     AWL  YH   V G QSS +  
Sbjct: 184 QAVGLLLALAAHFRGQIYWAKDIIFLVTDH---DLLGTEAWLEAYHDINVTGIQSSPLQ- 243

Query: 241 DACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNG 300
                                      RAG + AA+ +++S+       SL V  E  NG
Sbjct: 244 --------------------------GRAGAIQAAVALELSS---DVVTSLDVTVEGLNG 303

Query: 301 QMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKF 360
           Q+PNLDL+N+          F+   +K   L   +            GK+      +W  
Sbjct: 304 QLPNLDLLNL----------FQTFCQKGGLLCTLQ------------GKL---QPQDW-- 363

Query: 361 GMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDF 420
             S    + G  TL   +  QA G P G HG F  Y ++A+T+     F    + + D  
Sbjct: 364 -TSLEGPLQGLQTLLLMVLRQASGRPHGPHGLFLRYGVEALTLRGINSFR---QYKYD-- 423

Query: 421 ILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAA 480
           +   G+ +EG+ R +N+LLE+ HQSFF YL+ +  +FVS+G+YM A   L+  L   A  
Sbjct: 424 LATVGKALEGMFRKLNHLLERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALE 483

Query: 481 LYSYANNLNLTSEKV-EPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYF 540
           L+   +   +  E+  + P+   P    ++  +     +    + +    G  +  LP  
Sbjct: 484 LWMQLHQAGVNPEEAGKAPSPGTP----LLPTQGVGLASLTAPLLISQAMGLALYFLPVL 537

Query: 541 ICQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQE--WAFLKAMTTSA 600
              +             +L+LL++ V  + L  P ++ +    ++ +  W  LK +    
Sbjct: 544 GQHLATQHFPVAEAEAVVLTLLAIYVAGLAL--PHNTHRVVNSQVPDRGWMALKLVALIY 537

Query: 601 AFIGLCLMSVINFSTAELGAFLVVSMCLLAHP 628
             + L  ++++NFS   L A  +V    LA P
Sbjct: 604 LALQLGCIALLNFSLGFLLAATMVPAAALAKP 537

BLAST of Cucsat.G9697 vs. ExPASy Swiss-Prot
Match: O43292 (Glycosylphosphatidylinositol anchor attachment 1 protein OS=Homo sapiens OX=9606 GN=GPAA1 PE=1 SV=3)

HSP 1 Score: 107.8 bits (268), Expect = 4.9e-22
Identity = 131/496 (26.41%), Postives = 215/496 (43.35%), Query Frame = 0

Query: 143 MTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKD 202
           M  G N  GI+RAP+A   E++VL  P     T   ++ ++G+  ++ +      + AKD
Sbjct: 123 MVSGTNVYGILRAPRAASTESLVLTVPCGSDST---NSQAVGLLLALAAHFRGQIYWAKD 182

Query: 203 IVWLVADSRYGEYAAVSAWLRDYH-TPVFG-QSSVIDTDACSETNVLDEFEANQVTEKRI 262
           IV+LV +    +     AWL  YH   V G QSS +                        
Sbjct: 183 IVFLVTEH---DLLGTEAWLEAYHDVNVTGMQSSPLQ----------------------- 242

Query: 263 LDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFR 322
                RAG + AA+ +++S+       SL V  E  NGQ+PNLDL+N+          F+
Sbjct: 243 ----GRAGAIQAAVALELSS---DVVTSLDVAVEGLNGQLPNLDLLNL----------FQ 302

Query: 323 IKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGMSASDYVDGTATLASSLYYQA 382
              +K   L   +            GK+      +W    S    + G  TL   +  QA
Sbjct: 303 TFCQKGGLLCTLQ------------GKL---QPEDW---TSLDGPLQGLQTLLLMVLRQA 362

Query: 383 VGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFILRGGRLIEGVVRSVNNLLEKF 442
            G P GSHG F  Y+++A+T+     F    + + D  ++  G+ +EG+ R +N+LLE+ 
Sbjct: 363 SGRPHGSHGLFLRYRVEALTLRGINSFR---QYKYD--LVAVGKALEGMFRKLNHLLERL 422

Query: 443 HQSFFLYLMVSTGKFVSVGVYM--IAFALLVAPLPAVAAALYSYANNLNLTSEKVEP-PA 502
           HQSFFLYL+    +FVS+G+YM  + F LLV  L A+   +  +   + L      P P+
Sbjct: 423 HQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPS 482

Query: 503 LANPDDE------LIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQIPGYSPTENSI 562
           +  P  +      L+  L   + +  A  ++V+ + G  V+   + + +           
Sbjct: 483 VPLPPSQGVGLASLVAPLLISQAMGLA--LYVLPVLGQHVATQHFPVAEAEAV------- 537

Query: 563 IWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGLCLMSVINFSTA 622
              +L+LL++    + L        S +   + W  LK +      + L  +++ NFS  
Sbjct: 543 ---VLTLLAIYAAGLALPHNTHRVVSTQAPDRGWMALKLVALIYLALQLGCIALTNFSLG 537

Query: 623 ELGAFLVVSMCLLAHP 628
            L A  +V    LA P
Sbjct: 603 FLLATTMVPTAALAKP 537

BLAST of Cucsat.G9697 vs. ExPASy Swiss-Prot
Match: Q9US48 (GPI transamidase component gaa1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gaa1 PE=3 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 5.1e-19
Identity = 150/683 (21.96%), Postives = 263/683 (38.51%), Query Frame = 0

Query: 15  VRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAM--NTYISENALMPGSANSMLSGR--- 74
           +R+  FL  H  F  +     G+  + +LP   +    ++SE+AL+PG  N+    R   
Sbjct: 9   IRVFPFLQRHLFFLQLSLTLIGLSWIFILPRNEIIDRLHVSESALLPGQVNTYFENRYSK 68

Query: 75  EVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSSQFHPLHFFS 134
            VS +         L++  G++       L Q  + +G         P   Q + ++   
Sbjct: 69  TVSSSLTAANTWSHLDASVGTNTMYDD--LEQIFTAMGL--------PTQKQNYSINIPG 128

Query: 135 SPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDTLSLGIAYSI 194
           S               G N +  +RAP+ D  E+++L  P+       ++   + +A S+
Sbjct: 129 SE------------FNGSNFITTLRAPRGDATESLLLCVPWKD-HIGQYNEAGVALAISL 188

Query: 195 FSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDACSETNVLDE 254
                  +  +KDI+ ++ D                  PV+G SS + T    +T     
Sbjct: 189 LKYFQGWSLWSKDIILVIFDD-----------------PVYGPSSFL-TSYFDQTTPYIS 248

Query: 255 FEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQMPNLDLINIV 314
           +   ++          R+G++ A L +++   +E+  D L V  +A+NGQ+PNLDL N +
Sbjct: 249 YTPLKI----------RSGSIQAGLSLELVT-TENNSDVLEVLYQATNGQLPNLDLFNTI 308

Query: 315 NYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGMSASDYVDGT 374
           + + +    + ++++              G  F +                S S Y    
Sbjct: 309 SRIFMQHFNYPLRLQ--------------GYDFHA---------------NSGSSYTSRL 368

Query: 375 ATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDF---ILRGGRLI 434
            +L   +  QAV   T +H  F  Y+ID +T+ M  K         D F   + R G+ I
Sbjct: 369 KSLWMGMLTQAVSNVTSAHALFPQYRIDMLTLRMKVK---------DPFSFDMFRFGQAI 428

Query: 435 EGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALYSYANNL 494
           E   RS+NNLLE  HQSFF Y ++    F+S+G YM +  +L     A +  L +Y +  
Sbjct: 429 ESTFRSLNNLLEHLHQSFFFYFILDHLHFISIGNYMPSILIL-----AASFMLGAYRH-- 488

Query: 495 NLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQIPGYSP 554
                                      W+N  K+   + LW      L    C I  Y  
Sbjct: 489 ---------------------------WINHEKK---IDLWRPFSFWLFSIFCTIAAYYL 543

Query: 555 TENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGLCLMSVI 614
             +S    +   + L ++   +G  FS+  + E    E      + + + FI   ++S +
Sbjct: 549 VSSST--KITVFIFLYLMLTFIGIIFSTFMTSED--AELVLSYDLMSKSLFIS--VVSTL 543

Query: 615 NFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFFLFKGAL 674
           NFS + + A L+V +  ++                R    L L F      + F+F  +L
Sbjct: 609 NFSLSFVVAILLVPLQFISF---------------RFNRRLSLLFAVLTYFSTFIFLCSL 543

Query: 675 QGFDNLHIGDFWNWMETLWAWNS 690
               N  +  FW W +    +NS
Sbjct: 669 SKILNGPLVPFWLWAKEYELFNS 543

BLAST of Cucsat.G9697 vs. ExPASy Swiss-Prot
Match: P39012 (GPI transamidase component GAA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GAA1 PE=1 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.8e-16
Identity = 124/511 (24.27%), Postives = 213/511 (41.68%), Query Frame = 0

Query: 1   MAETEKLKPKARPIVRLGIF--LISHSIFFSVVCFSAGVLALLLLPMFAM--NTYISENA 60
           MA  EKL    R IV +G+   +I+     S++C   G +++ +LPM      TYISENA
Sbjct: 1   MALLEKLH---RRIVDMGLVPRIIALLPVISMLCALFGFISIAILPMDGQYRRTYISENA 60

Query: 61  LMPGSANSMLSGRE---VSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYH 120
           LMP  A S     E   +    + +K++  + S   +++ GS      ++ + G     +
Sbjct: 61  LMPSQAYSYFRESEWNILRGYRSQIKEMVNMTSMERNNLMGS------WLQEFGTKTAIY 120

Query: 121 RFHPQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTP-YNP 180
               ++ Q+                       G    G++ AP+ DG EA+VL  P +N 
Sbjct: 121 ----ENEQY-----------------------GETLYGVMHAPRGDGTEAMVLAVPWFNS 180

Query: 181 VETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQ 240
            +       +LG++ + F     V W +K+I+ + +++     AA+ +W+  YHT     
Sbjct: 181 DDEFNIGGAALGVSLARFFSRWPV-W-SKNIIVVFSEN---PRAALRSWVEAYHTS---- 240

Query: 241 SSVIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVY 300
                                       LD     G++ AA+V+  S+  + FE  + + 
Sbjct: 241 ----------------------------LD--LTGGSIEAAVVLDYSSTEDFFE-YVEIS 300

Query: 301 AEASNGQMPNLDLINIVNYLAVHRQGFRIKI----------EKFWPLLNCKWLKVLGEVF 360
            +  NG++PNLDL+NI   +  H +G ++ +            FW  L    L +     
Sbjct: 301 YDGLNGELPNLDLVNIAISITEH-EGMKVSLHGLPSDQLTNNNFWSRLKILCLGIRDWAL 360

Query: 361 ESIGKVIRSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVE 420
             + K                                    P G+  AF  ++I ++T++
Sbjct: 361 SGVKK------------------------------------PHGNE-AFSGWRIQSVTLK 389

Query: 421 MSPKFSSGIKVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYM 480
                   I           GR+ E + RS+NNLLEKFHQSFF YL+++  +FVS+  Y+
Sbjct: 421 AHGNSGHDITT--------FGRIPEAMFRSINNLLEKFHQSFFFYLLLAPRQFVSISSYL 389

Query: 481 -IAFALLVA-PLPAVAAALYSYANNLNLTSE 492
             A AL +A  + ++ A + +   N++L SE
Sbjct: 481 PSAVALSIAFAISSLNAFINNAYANISLFSE 389

BLAST of Cucsat.G9697 vs. NCBI nr
Match: XP_004142789.2 (glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Cucumis sativus] >KGN51360.1 hypothetical protein Csa_009006 [Cucumis sativus])

HSP 1 Score: 1369 bits (3544), Expect = 0.0
Identity = 697/697 (100.00%), Postives = 697/697 (100.00%), Query Frame = 0

Query: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120
           SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS
Sbjct: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120

Query: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180
           QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT
Sbjct: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240
           LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240

Query: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360
           PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM
Sbjct: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360

Query: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420
           SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL
Sbjct: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480

Query: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540
           SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ
Sbjct: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540

Query: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660
           CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660

Query: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 697
           LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM
Sbjct: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 697

BLAST of Cucsat.G9697 vs. NCBI nr
Match: KAA0037577.1 (glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1324 bits (3426), Expect = 0.0
Identity = 671/697 (96.27%), Postives = 686/697 (98.42%), Query Frame = 0

Query: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKLK KARPIVRLGIFLISHSIFFSV+CFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKLKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120
           SAN+MLSGREVSEANNLVKDLKGLNSKPGSSIF SQQILA+YISKLGADVNYHRFH QSS
Sbjct: 61  SANTMLSGREVSEANNLVKDLKGLNSKPGSSIFESQQILARYISKLGADVNYHRFHAQSS 120

Query: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180
           QFHPLHFFSSPDSGIL+DNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT
Sbjct: 121 QFHPLHFFSSPDSGILQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240
           LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQS VIDTDA
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSGVIDTDA 240

Query: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           C+ETNVL+ FEAN VTEK ILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CTETNVLNGFEANHVTEKWILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360
           PNLDLINIVNYLAVHRQGFRIKIEKFWPLL+CKWLKVLGEVFESIGK+IRSLNSEWKFGM
Sbjct: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGKIIRSLNSEWKFGM 360

Query: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420
           SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSG+KVRRDDFIL
Sbjct: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDDFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480

Query: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540
           S+ANNLNLTSEKVEPPA ANPDDELIVSLRSWKWLNAAKRVFVVHLWGA+VSLLPYFICQ
Sbjct: 481 SHANNLNLTSEKVEPPAPANPDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540

Query: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPTENSIIWGLLSLL+LLVLSVILGSP SSTKSYEQ IQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTENSIIWGLLSLLALLVLSVILGSPLSSTKSYEQGIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660
           CLMSVINF+TAE+GAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAEIGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660

Query: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 697
           LFK ALQGFDNLHIGDFWNWMETLWAWNSATFLYLG+
Sbjct: 661 LFKAALQGFDNLHIGDFWNWMETLWAWNSATFLYLGL 697

BLAST of Cucsat.G9697 vs. NCBI nr
Match: XP_008458804.1 (PREDICTED: glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Cucumis melo])

HSP 1 Score: 1321 bits (3418), Expect = 0.0
Identity = 669/697 (95.98%), Postives = 684/697 (98.13%), Query Frame = 0

Query: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKLK KARPIVRLGIFLISHSIFFSV+CFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKLKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120
           SAN+MLSGREVSEANNLVKDLKGLNSKPGSSIF SQQILA+YISKLGADVNYHRFH QSS
Sbjct: 61  SANTMLSGREVSEANNLVKDLKGLNSKPGSSIFESQQILARYISKLGADVNYHRFHAQSS 120

Query: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180
           QFHPLHFFSSPDSGIL+DNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT
Sbjct: 121 QFHPLHFFSSPDSGILQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240
           LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQS VIDTDA
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSGVIDTDA 240

Query: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           C+ETNVL+ FEAN VTEK ILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CTETNVLNGFEANHVTEKWILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360
           PNLDLINIVNYLAVHRQGFRIKIEKFWPLL+CKWLKVLGEVFESIGK+IRSLNSEWKFGM
Sbjct: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGKIIRSLNSEWKFGM 360

Query: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420
           SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSG+KVRRDDFIL
Sbjct: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDDFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480
           RGGRL+EGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY
Sbjct: 421 RGGRLVEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480

Query: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540
           S+ANNLNLTSEKVEPPA  NPDDELIVSLRSWKWLNAAKRVFVVHLWGA+VSLLPYFICQ
Sbjct: 481 SHANNLNLTSEKVEPPAPENPDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540

Query: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPTENSIIWGLLSLL+LLVLSVILG P SSTKSYEQ IQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTENSIIWGLLSLLALLVLSVILGCPLSSTKSYEQGIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660
           CLMSVINF+TAE+GAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAEIGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660

Query: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 697
           LFK ALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM
Sbjct: 661 LFKAALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 697

BLAST of Cucsat.G9697 vs. NCBI nr
Match: TYK03095.1 (glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1315 bits (3404), Expect = 0.0
Identity = 671/708 (94.77%), Postives = 686/708 (96.89%), Query Frame = 0

Query: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKLK KARPIVRLGIFLISHSIFFSV+CFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKLKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  -----------SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGAD 120
                      SAN+MLSGREVSEANNLVKDLKGLNSKPGSSIF SQQILA+YISKLGAD
Sbjct: 61  LLTVVLYFPIGSANTMLSGREVSEANNLVKDLKGLNSKPGSSIFESQQILARYISKLGAD 120

Query: 121 VNYHRFHPQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTP 180
           VNYHRFH QSSQFHPLHFFSSPDSGIL+DNVSCMTQGINTVGIIRAPQADGKEAIVLVTP
Sbjct: 121 VNYHRFHAQSSQFHPLHFFSSPDSGILQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTP 180

Query: 181 YNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPV 240
           YNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPV
Sbjct: 181 YNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPV 240

Query: 241 FGQSSVIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSL 300
           FGQS VIDTDAC+ETNVL+ FEAN VTEK ILDDFKRAGTMAAALVIKVSNRSEHFEDSL
Sbjct: 241 FGQSGVIDTDACTETNVLNGFEANHVTEKWILDDFKRAGTMAAALVIKVSNRSEHFEDSL 300

Query: 301 SVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVI 360
           SVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLL+CKWLKVLGEVFESIGK+I
Sbjct: 301 SVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGKII 360

Query: 361 RSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSS 420
           RSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSS
Sbjct: 361 RSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSS 420

Query: 421 GIKVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLV 480
           G+KVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLV
Sbjct: 421 GVKVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLV 480

Query: 481 APLPAVAAALYSYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGA 540
           APLPAVAAALYS+ANNLNLTSEKVEPPA ANPDDELIVSLRSWKWLNAAKRVFVVHLWGA
Sbjct: 481 APLPAVAAALYSHANNLNLTSEKVEPPAPANPDDELIVSLRSWKWLNAAKRVFVVHLWGA 540

Query: 541 IVSLLPYFICQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLK 600
           +VSLLPYFICQIPGYSPTENSIIWGLLSLL+LLVLSVILGSP SSTKSYEQ IQEWAFLK
Sbjct: 541 VVSLLPYFICQIPGYSPTENSIIWGLLSLLALLVLSVILGSPLSSTKSYEQGIQEWAFLK 600

Query: 601 AMTTSAAFIGLCLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVL 660
           AMTTSAAFIGLCLMSVINF+TAE+GAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVL
Sbjct: 601 AMTTSAAFIGLCLMSVINFATAEIGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVL 660

Query: 661 GFIAFPPVTFFLFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 697
           GFIAFPPVTFFLFK ALQGFDNLHIGDFWNWMETLWAWNSATFLYLG+
Sbjct: 661 GFIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLWAWNSATFLYLGL 708

BLAST of Cucsat.G9697 vs. NCBI nr
Match: XP_011655396.1 (glycosylphosphatidylinositol anchor attachment 1 protein isoform X2 [Cucumis sativus])

HSP 1 Score: 1291 bits (3340), Expect = 0.0
Identity = 653/653 (100.00%), Postives = 653/653 (100.00%), Query Frame = 0

Query: 45  MFAMNTYISENALMPGSANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYIS 104
           MFAMNTYISENALMPGSANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYIS
Sbjct: 1   MFAMNTYISENALMPGSANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYIS 60

Query: 105 KLGADVNYHRFHPQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAI 164
           KLGADVNYHRFHPQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAI
Sbjct: 61  KLGADVNYHRFHPQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAI 120

Query: 165 VLVTPYNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRD 224
           VLVTPYNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRD
Sbjct: 121 VLVTPYNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRD 180

Query: 225 YHTPVFGQSSVIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEH 284
           YHTPVFGQSSVIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEH
Sbjct: 181 YHTPVFGQSSVIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEH 240

Query: 285 FEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFES 344
           FEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFES
Sbjct: 241 FEDSLSVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFES 300

Query: 345 IGKVIRSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMS 404
           IGKVIRSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMS
Sbjct: 301 IGKVIRSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMS 360

Query: 405 PKFSSGIKVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIA 464
           PKFSSGIKVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIA
Sbjct: 361 PKFSSGIKVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIA 420

Query: 465 FALLVAPLPAVAAALYSYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVV 524
           FALLVAPLPAVAAALYSYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVV
Sbjct: 421 FALLVAPLPAVAAALYSYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVV 480

Query: 525 HLWGAIVSLLPYFICQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQE 584
           HLWGAIVSLLPYFICQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQE
Sbjct: 481 HLWGAIVSLLPYFICQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQE 540

Query: 585 WAFLKAMTTSAAFIGLCLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAA 644
           WAFLKAMTTSAAFIGLCLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAA
Sbjct: 541 WAFLKAMTTSAAFIGLCLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAA 600

Query: 645 CNLVLGFIAFPPVTFFLFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 697
           CNLVLGFIAFPPVTFFLFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM
Sbjct: 601 CNLVLGFIAFPPVTFFLFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 653

BLAST of Cucsat.G9697 vs. ExPASy TrEMBL
Match: A0A0A0KP05 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523060 PE=4 SV=1)

HSP 1 Score: 1369 bits (3544), Expect = 0.0
Identity = 697/697 (100.00%), Postives = 697/697 (100.00%), Query Frame = 0

Query: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120
           SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS
Sbjct: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120

Query: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180
           QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT
Sbjct: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240
           LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240

Query: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360
           PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM
Sbjct: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360

Query: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420
           SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL
Sbjct: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480

Query: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540
           SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ
Sbjct: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540

Query: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660
           CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660

Query: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 697
           LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM
Sbjct: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 697

BLAST of Cucsat.G9697 vs. ExPASy TrEMBL
Match: A0A5A7T7Q0 (Glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G002100 PE=4 SV=1)

HSP 1 Score: 1324 bits (3426), Expect = 0.0
Identity = 671/697 (96.27%), Postives = 686/697 (98.42%), Query Frame = 0

Query: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKLK KARPIVRLGIFLISHSIFFSV+CFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKLKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120
           SAN+MLSGREVSEANNLVKDLKGLNSKPGSSIF SQQILA+YISKLGADVNYHRFH QSS
Sbjct: 61  SANTMLSGREVSEANNLVKDLKGLNSKPGSSIFESQQILARYISKLGADVNYHRFHAQSS 120

Query: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180
           QFHPLHFFSSPDSGIL+DNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT
Sbjct: 121 QFHPLHFFSSPDSGILQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240
           LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQS VIDTDA
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSGVIDTDA 240

Query: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           C+ETNVL+ FEAN VTEK ILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CTETNVLNGFEANHVTEKWILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360
           PNLDLINIVNYLAVHRQGFRIKIEKFWPLL+CKWLKVLGEVFESIGK+IRSLNSEWKFGM
Sbjct: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGKIIRSLNSEWKFGM 360

Query: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420
           SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSG+KVRRDDFIL
Sbjct: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDDFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480

Query: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540
           S+ANNLNLTSEKVEPPA ANPDDELIVSLRSWKWLNAAKRVFVVHLWGA+VSLLPYFICQ
Sbjct: 481 SHANNLNLTSEKVEPPAPANPDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540

Query: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPTENSIIWGLLSLL+LLVLSVILGSP SSTKSYEQ IQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTENSIIWGLLSLLALLVLSVILGSPLSSTKSYEQGIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660
           CLMSVINF+TAE+GAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAEIGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660

Query: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 697
           LFK ALQGFDNLHIGDFWNWMETLWAWNSATFLYLG+
Sbjct: 661 LFKAALQGFDNLHIGDFWNWMETLWAWNSATFLYLGL 697

BLAST of Cucsat.G9697 vs. ExPASy TrEMBL
Match: A0A1S3C9A0 (glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498104 PE=4 SV=1)

HSP 1 Score: 1321 bits (3418), Expect = 0.0
Identity = 669/697 (95.98%), Postives = 684/697 (98.13%), Query Frame = 0

Query: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKLK KARPIVRLGIFLISHSIFFSV+CFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKLKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120
           SAN+MLSGREVSEANNLVKDLKGLNSKPGSSIF SQQILA+YISKLGADVNYHRFH QSS
Sbjct: 61  SANTMLSGREVSEANNLVKDLKGLNSKPGSSIFESQQILARYISKLGADVNYHRFHAQSS 120

Query: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180
           QFHPLHFFSSPDSGIL+DNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT
Sbjct: 121 QFHPLHFFSSPDSGILQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240
           LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQS VIDTDA
Sbjct: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSGVIDTDA 240

Query: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           C+ETNVL+ FEAN VTEK ILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CTETNVLNGFEANHVTEKWILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360
           PNLDLINIVNYLAVHRQGFRIKIEKFWPLL+CKWLKVLGEVFESIGK+IRSLNSEWKFGM
Sbjct: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGKIIRSLNSEWKFGM 360

Query: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420
           SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSG+KVRRDDFIL
Sbjct: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDDFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480
           RGGRL+EGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY
Sbjct: 421 RGGRLVEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480

Query: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540
           S+ANNLNLTSEKVEPPA  NPDDELIVSLRSWKWLNAAKRVFVVHLWGA+VSLLPYFICQ
Sbjct: 481 SHANNLNLTSEKVEPPAPENPDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFICQ 540

Query: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPTENSIIWGLLSLL+LLVLSVILG P SSTKSYEQ IQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTENSIIWGLLSLLALLVLSVILGCPLSSTKSYEQGIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660
           CLMSVINF+TAE+GAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAEIGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660

Query: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 697
           LFK ALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM
Sbjct: 661 LFKAALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 697

BLAST of Cucsat.G9697 vs. ExPASy TrEMBL
Match: A0A5D3BVI8 (Glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00170 PE=4 SV=1)

HSP 1 Score: 1315 bits (3404), Expect = 0.0
Identity = 671/708 (94.77%), Postives = 686/708 (96.89%), Query Frame = 0

Query: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEKLK KARPIVRLGIFLISHSIFFSV+CFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKLKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  -----------SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGAD 120
                      SAN+MLSGREVSEANNLVKDLKGLNSKPGSSIF SQQILA+YISKLGAD
Sbjct: 61  LLTVVLYFPIGSANTMLSGREVSEANNLVKDLKGLNSKPGSSIFESQQILARYISKLGAD 120

Query: 121 VNYHRFHPQSSQFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTP 180
           VNYHRFH QSSQFHPLHFFSSPDSGIL+DNVSCMTQGINTVGIIRAPQADGKEAIVLVTP
Sbjct: 121 VNYHRFHAQSSQFHPLHFFSSPDSGILQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTP 180

Query: 181 YNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPV 240
           YNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPV
Sbjct: 181 YNPVETSLHDTLSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPV 240

Query: 241 FGQSSVIDTDACSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSL 300
           FGQS VIDTDAC+ETNVL+ FEAN VTEK ILDDFKRAGTMAAALVIKVSNRSEHFEDSL
Sbjct: 241 FGQSGVIDTDACTETNVLNGFEANHVTEKWILDDFKRAGTMAAALVIKVSNRSEHFEDSL 300

Query: 301 SVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVI 360
           SVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLL+CKWLKVLGEVFESIGK+I
Sbjct: 301 SVYAEASNGQMPNLDLINIVNYLAVHRQGFRIKIEKFWPLLDCKWLKVLGEVFESIGKII 360

Query: 361 RSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSS 420
           RSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSS
Sbjct: 361 RSLNSEWKFGMSASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSS 420

Query: 421 GIKVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLV 480
           G+KVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLV
Sbjct: 421 GVKVRRDDFILRGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLV 480

Query: 481 APLPAVAAALYSYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGA 540
           APLPAVAAALYS+ANNLNLTSEKVEPPA ANPDDELIVSLRSWKWLNAAKRVFVVHLWGA
Sbjct: 481 APLPAVAAALYSHANNLNLTSEKVEPPAPANPDDELIVSLRSWKWLNAAKRVFVVHLWGA 540

Query: 541 IVSLLPYFICQIPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLK 600
           +VSLLPYFICQIPGYSPTENSIIWGLLSLL+LLVLSVILGSP SSTKSYEQ IQEWAFLK
Sbjct: 541 VVSLLPYFICQIPGYSPTENSIIWGLLSLLALLVLSVILGSPLSSTKSYEQGIQEWAFLK 600

Query: 601 AMTTSAAFIGLCLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVL 660
           AMTTSAAFIGLCLMSVINF+TAE+GAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVL
Sbjct: 601 AMTTSAAFIGLCLMSVINFATAEIGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVL 660

Query: 661 GFIAFPPVTFFLFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 697
           GFIAFPPVTFFLFK ALQGFDNLHIGDFWNWMETLWAWNSATFLYLG+
Sbjct: 661 GFIAFPPVTFFLFKAALQGFDNLHIGDFWNWMETLWAWNSATFLYLGL 708

BLAST of Cucsat.G9697 vs. ExPASy TrEMBL
Match: A0A6J1K1W5 (glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489371 PE=4 SV=1)

HSP 1 Score: 1259 bits (3258), Expect = 0.0
Identity = 640/697 (91.82%), Postives = 665/697 (95.41%), Query Frame = 0

Query: 1   MAETEKLKPKARPIVRLGIFLISHSIFFSVVCFSAGVLALLLLPMFAMNTYISENALMPG 60
           MAETEK K KARPIVRLGIFLISHSIFFSV+CFSAGVLALLLLPMFAMNTYISENALMPG
Sbjct: 1   MAETEKPKTKARPIVRLGIFLISHSIFFSVICFSAGVLALLLLPMFAMNTYISENALMPG 60

Query: 61  SANSMLSGREVSEANNLVKDLKGLNSKPGSSIFGSQQILAQYISKLGADVNYHRFHPQSS 120
           SAN+MLSG+EVSEAN+LVKDLKGLNSK GS+IFGSQQILAQYIS LGA+VNYHRF PQSS
Sbjct: 61  SANTMLSGQEVSEANDLVKDLKGLNSKSGSTIFGSQQILAQYISNLGAEVNYHRFQPQSS 120

Query: 121 QFHPLHFFSSPDSGILKDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVETSLHDT 180
           QFHPLHFFSSPDSGI++DNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPV+TSLH+T
Sbjct: 121 QFHPLHFFSSPDSGIVQDNVSCMTQGINTVGIIRAPQADGKEAIVLVTPYNPVKTSLHET 180

Query: 181 LSLGIAYSIFSLLTQVTWLAKDIVWLVADSRYGEYAAVSAWLRDYHTPVFGQSSVIDTDA 240
           LSLGIAYSIFSLLTQV+WLAKDIVWL ADSRYGEYAAVSAWLRDYHTP FG+S++IDT  
Sbjct: 181 LSLGIAYSIFSLLTQVSWLAKDIVWLAADSRYGEYAAVSAWLRDYHTPSFGRSNMIDTYT 240

Query: 241 CSETNVLDEFEANQVTEKRILDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300
           C+ETNVL EFEAN VTEK  LDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM
Sbjct: 241 CAETNVLYEFEANHVTEKESLDDFKRAGTMAAALVIKVSNRSEHFEDSLSVYAEASNGQM 300

Query: 301 PNLDLINIVNYLAVHRQGFRIKIEKFWPLLNCKWLKVLGEVFESIGKVIRSLNSEWKFGM 360
           PNLDLINIVNYLA HRQGFRIKIEKFWPLL+CKWLK LGE FESIGK IRSLN EWKFGM
Sbjct: 301 PNLDLINIVNYLAEHRQGFRIKIEKFWPLLDCKWLKFLGEFFESIGKFIRSLNPEWKFGM 360

Query: 361 SASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGIKVRRDDFIL 420
            ASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSG+KVRRD+FIL
Sbjct: 361 LASDYVDGTATLASSLYYQAVGIPTGSHGAFRDYQIDAITVEMSPKFSSGVKVRRDEFIL 420

Query: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTGKFVSVGVYMIAFALLVAPLPAVAAALY 480
           RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVST KFVSVGVYMIAFALLVAPLPAVAA+LY
Sbjct: 421 RGGRLIEGVVRSVNNLLEKFHQSFFLYLMVSTSKFVSVGVYMIAFALLVAPLPAVAASLY 480

Query: 481 SYANNLNLTSEKVEPPALANPDDELIVSLRSWKWLNAAKRVFVVHLWGAIVSLLPYFICQ 540
           SYANN NLT EK+EP A AN DDELIVSLRSWKWLNAAKRVFVVHLWGA+VSLLPYFI Q
Sbjct: 481 SYANNSNLTLEKIEPAAPANSDDELIVSLRSWKWLNAAKRVFVVHLWGAVVSLLPYFISQ 540

Query: 541 IPGYSPTENSIIWGLLSLLSLLVLSVILGSPFSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600
           IPGYSPT NSIIW LLSLL+LL+LSVILGSP SSTKSYEQRIQEWAFLKAMTTSAAFIGL
Sbjct: 541 IPGYSPTTNSIIWALLSLLTLLILSVILGSPLSSTKSYEQRIQEWAFLKAMTTSAAFIGL 600

Query: 601 CLMSVINFSTAELGAFLVVSMCLLAHPLKLDLGAGNFKALSRAACNLVLGFIAFPPVTFF 660
           CLMSVINF+TAELGAFLVVSMCLLAHPLKLDL AGNFKALSRAA NLVLGFIAFPPVTFF
Sbjct: 601 CLMSVINFATAELGAFLVVSMCLLAHPLKLDLVAGNFKALSRAASNLVLGFIAFPPVTFF 660

Query: 661 LFKGALQGFDNLHIGDFWNWMETLWAWNSATFLYLGM 697
           LFK ALQGFDNLH+GDFWNWMETLWAWNSATFLYLGM
Sbjct: 661 LFKAALQGFDNLHLGDFWNWMETLWAWNSATFLYLGM 697

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9WTK34.5e-2324.05Glycosylphosphatidylinositol anchor attachment 1 protein OS=Mus musculus OX=1009... [more]
O432924.9e-2226.41Glycosylphosphatidylinositol anchor attachment 1 protein OS=Homo sapiens OX=9606... [more]
Q9US485.1e-1921.96GPI transamidase component gaa1 OS=Schizosaccharomyces pombe (strain 972 / ATCC ... [more]
P390121.8e-1624.27GPI transamidase component GAA1 OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
Match NameE-valueIdentityDescription
XP_004142789.20.0100.00glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Cucumis sat... [more]
KAA0037577.10.096.27glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Cucumis mel... [more]
XP_008458804.10.095.98PREDICTED: glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [... [more]
TYK03095.10.094.77glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 [Cucumis mel... [more]
XP_011655396.10.0100.00glycosylphosphatidylinositol anchor attachment 1 protein isoform X2 [Cucumis sat... [more]
Match NameE-valueIdentityDescription
A0A0A0KP050.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523060 PE=4 SV=1[more]
A0A5A7T7Q00.096.27Glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 OS=Cucumis m... [more]
A0A1S3C9A00.095.98glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 OS=Cucumis m... [more]
A0A5D3BVI80.094.77Glycosylphosphatidylinositol anchor attachment 1 protein isoform X1 OS=Cucumis m... [more]
A0A6J1K1W50.091.82glycosylphosphatidylinositol anchor attachment 1 protein-like isoform X1 OS=Cucu... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007246GPI transamidase component Gaa1PIRSFPIRSF036762GAA1coord: 1..670
e-value: 8.4E-186
score: 617.0
IPR007246GPI transamidase component Gaa1PFAMPF04114Gaa1coord: 101..662
e-value: 2.3E-110
score: 370.0
IPR007246GPI transamidase component Gaa1PANTHERPTHR13304GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEINcoord: 2..667
NoneNo IPR availablePANTHERPTHR13304:SF0GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEINcoord: 2..667

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G9697.T1Cucsat.G9697.T1mRNA
Cucsat.G9697.T10Cucsat.G9697.T10mRNA
Cucsat.G9697.T2Cucsat.G9697.T2mRNA
Cucsat.G9697.T7Cucsat.G9697.T7mRNA
Cucsat.G9697.T6Cucsat.G9697.T6mRNA
Cucsat.G9697.T5Cucsat.G9697.T5mRNA
Cucsat.G9697.T4Cucsat.G9697.T4mRNA
Cucsat.G9697.T3Cucsat.G9697.T3mRNA
Cucsat.G9697.T9Cucsat.G9697.T9mRNA
Cucsat.G9697.T8Cucsat.G9697.T8mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016255 attachment of GPI anchor to protein
cellular_component GO:0042765 GPI-anchor transamidase complex
cellular_component GO:0016021 integral component of membrane