Cucsat.G9573 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G9573
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Small heat shock protein
Location	ctg1673: 2779553 .. 2781251 (-)
RNA-Seq Expression	Cucsat.G9573
Synteny	Cucsat.G9573

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTATGAGTAAAAAAAGTTAAGTTTACTCAACCGATCTCGAGGTTGAGTTTGACAGAGATTGACACAAAAGGATATAAATTCTGATCATTCATACCTACTTTTCCGATGTGGATGCTCACCTTAGAACCATAAAAATACAAGCTCGCGTGTAGAGATACACACAAATCATTAGATGCTTCTAGGAGGTGTAGTTGATATATTCAATATAGAAAATAGAGGATAATTCTGTTGGATGGCTCAAATTTCTGGAGAGATCTAGAACGAAAGGAGGTTCCAGTTCTTTCCATCTCCAGACCCTCCTTCTCCATCCTTTAATAACTTCAACAATGGCGATTTTCTCATCACCTAAACGTTCCTTAACACAATCCTCATTCCTACTACTTCCTCCAACTCAATTCGAACTTCACATTTCTCTTTTAGTCCACAGATTCCTACATAATCCACAAAGTTCTGTTTGAATGGCTCAAGCTGTATCAAATCTGACTCGATTCGTCTGCTTCACTCCTACCAAAGCCTCCAATGATCCCAAGAAGTTTGTTCGTAAAAGTAGCGTCAAAGCCGTGAGAGGGGATGGCAGAGAGAATCTCGATCACTTACAGAGAACTATCGAAAAGGAAAAACAAACAGCTCAGCCACCGAAGAAACGAGTAGCTCCTGTTGCTCCAATTGGTAATTGATCTGACCTTATTGCTGGTTAAAATTAATGGGCAACTCTTCTTTTTGGTTTTGGTTTGATTTTGTTTTTTGTTTGTGTTCGTTTTTTCTAGGGCTTTGGGATCGATTTCCGACGGCGAGAACTGTACAGCAAATGATGGAGACAATGGAGAGGATAATGGATGATCCTTTTGCTTACTCGGGAGCATGGCCATCGCCTCCATTTACCAGCGATGGTGCTGGGTACAGTAGGGGAAGAACTCCATGGGAGATCAAGGAAGGGGAAAATGAGTATAAGATGAGGTTTGACATGCCGGGTATGACGAAGGACGATGTGAAGGTGTGGGTGGAGGAGAAGATGTTGGTTGTGAAGGCTGAGAAGGCTGAGAAGGTGAAGAAGGCTAGTGAAGAAAATGGGAAGGTTGAAGAAGAAGAGGGAGATTGGTCTGCTAAAAGCTATGGGAGATACAGTAGCAGAATTGCTTTGCCTGAGAATGTTCAATTTGAGCAGATTAAGGCAGAGGTAAAAGATGGGGTTTTGTACATAACAATACCAAAGGCTATGGCTACAAGTAAAATTGTGGACATTAATGTCCAGTGAAGAGTTGAGATTAGATTTCGAGAGTAGGTGTAGTTATTTTTTTATCTGCTTTCAGTTTCAAAAGAACCTGATGTAAACAGTTATGAAGATGAAGTTCTTGCAATTTCATCCTGGCTTTTGTAATCTATTTTCAACAAAGCTTCGATGTAAACATTTGGCATTACTGATGGAATGATACCTTTAGTTACCTGTTAAACGCAACTGCTTTGAATTTTGTTTTAAGAGAACTGGTTCATCAGAATTTTGAAGAACCGTGGTTCCTTCTGTTATTGTCTGGATTGAATATTAGTAGCTGAAACGTTACTTCAGGAGTTATGTTCTTACTTTCTTGCTTTCAGAGAACCAAGCTCAACATGAAAGCCATGTAGTTTTAGAGAGATGAATCAGAATGCCAACCTTCAGGTGTAAGTTGTTTTCAGATGATCTCTTATCTCTATCTGTTG

Coding sequence (CDS)

ATGGCTCAAGCTGTATCAAATCTGACTCGATTCGTCTGCTTCACTCCTACCAAAGCCTCCAATGATCCCAAGAAGTTTGTTCGTAAAAGTAGCGTCAAAGCCGTGAGAGGGGATGGCAGAGAGAATCTCGATCACTTACAGAGAACTATCGAAAAGGAAAAACAAACAGCTCAGCCACCGAAGAAACGAGTAGCTCCTGTTGCTCCAATTGGGCTTTGGGATCGATTTCCGACGGCGAGAACTGTACAGCAAATGATGGAGACAATGGAGAGGATAATGGATGATCCTTTTGCTTACTCGGGAGCATGGCCATCGCCTCCATTTACCAGCGATGGTGCTGGGTACAGTAGGGGAAGAACTCCATGGGAGATCAAGGAAGGGGAAAATGAGTATAAGATGAGGTTTGACATGCCGGGTATGACGAAGGACGATGTGAAGGTGTGGGTGGAGGAGAAGATGTTGGTTGTGAAGGCTGAGAAGGCTGAGAAGGTGAAGAAGGCTAGTGAAGAAAATGGGAAGGTTGAAGAAGAAGAGGGAGATTGGTCTGCTAAAAGCTATGGGAGATACAGTAGCAGAATTGCTTTGCCTGAGAATGTTCAATTTGAGCAGATTAAGGCAGAGGTAAAAGATGGGGTTTTGTACATAACAATACCAAAGGCTATGGCTACAAGTAAAATTGTGGACATTAATGTCCAGTGA

Protein sequence

MAQAVSNLTRFVCFTPTKASNDPKKFVRKSSVKAVRGDGRENLDHLQRTIEKEKQTAQPPKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSRGRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKVEEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ

Homology

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BLAST of Cucsat.G9573 vs. ExPASy Swiss-Prot
Match: P31170 (Heat shock protein 21, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HSP21 PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 7.8e-33
Identity = 82/212 (38.68%), Postives = 126/212 (59.43%), Query Frame = 0


Query: 26  FVRKSSVKAVRGDGREN-LDHLQRTIEKEKQTA----QPPKKRVAPVAPIGLWDRFPTAR 85
           F RK   +    D REN +D +Q+  +K  Q +    +P ++    V+P GL D     R
Sbjct: 34  FPRKIPSRIRAQDQRENSIDVVQQGQQKGNQGSSVEKRPQQRLTMDVSPFGLLDPLSPMR 93

Query: 86  TVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSRGRTPWEIKEGENEYKMRFDMPGM 145
           T++QM++TM+R+ +D    SG          G+G S  R PW+IKE E+E KMRFDMPG+
Sbjct: 94  TMRQMLDTMDRMFEDTMPVSGR------NRGGSGVSEIRAPWDIKEEEHEIKMRFDMPGL 153

Query: 146 TKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKVEEEEGDWSAKSYGRYSSRIALPENVQ 205
           +K+DVK+ VE+ +LV+K E+            K E+ +  WS +S   Y +R+ LP+N +
Sbjct: 154 SKEDVKISVEDNVLVIKGEQ------------KKEDSDDSWSGRSVSSYGTRLQLPDNCE 213

Query: 206 FEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 233
            ++IKAE+K+GVL+ITIPK     K++D+ +Q
Sbjct: 214 KDKIKAELKNGVLFITIPKTKVERKVIDVQIQ 227

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BLAST of Cucsat.G9573 vs. ExPASy Swiss-Prot
Match: P09886 (Small heat shock protein, chloroplastic OS=Pisum sativum OX=3888 GN=HSP21 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.5e-31
Identity = 78/198 (39.39%), Postives = 119/198 (60.10%), Query Frame = 0


Query: 37  GDGRENLDHLQRTIEKEKQTA--QPPKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMD 96
           GD ++N   + R  + ++ TA  + P++    ++P GL D +   R+++QM++TM+RI +
Sbjct: 55  GDNKDNSVEVHRVNKDDQGTAVERKPRRSSIDISPFGLLDPWSPMRSMRQMLDTMDRIFE 114

Query: 97  DPFAYSGAWPSPPFTSDGAGYSRGRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKML 156
           D     G            G    R PWEIK+ E+E +MRFDMPG++K+DVKV VE+ +L
Sbjct: 115 DAITIPGR---------NIGGGEIRVPWEIKDEEHEIRMRFDMPGVSKEDVKVSVEDDVL 174

Query: 157 VVKAEKAEKVKKASEENGKVEEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLY 216
           V+K++         EENG     E  WS KSY  Y +R+ LP+N + E++KAE+KDGVLY
Sbjct: 175 VIKSDH-------REENG----GEDCWSRKSYSCYDTRLKLPDNCEKEKVKAELKDGVLY 232

Query: 217 ITIPKAMATSKIVDINVQ 233
           ITIPK      ++D+ +Q
Sbjct: 235 ITIPKTKIERTVIDVQIQ 232

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BLAST of Cucsat.G9573 vs. ExPASy Swiss-Prot
Match: P09887 (Small heat shock protein, chloroplastic (Fragment) OS=Glycine max OX=3847 GN=HSP22 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.6e-30
Identity = 71/198 (35.86%), Postives = 121/198 (61.11%), Query Frame = 0


Query: 37  GDGRENLDHLQRTIEKEKQTA--QPPKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMD 96
           GD ++N   +Q   + ++ TA  + P++    ++P G+ D +   R+++Q+++TM+R+ +
Sbjct: 2   GDNKDNSVEVQHVSKGDQGTAVEKKPRRTAMDISPFGILDPWSPMRSMRQILDTMDRVFE 61

Query: 97  DPFAYSGAWPSPPFTSDGAGYSRGRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKML 156
           D   + G            G    R PW+IK+ E+E +MRFDMPG+ K+DVKV VE+ ML
Sbjct: 62  DTMTFPGR---------NIGGGEIRAPWDIKDEEHEIRMRFDMPGLAKEDVKVSVEDDML 121

Query: 157 VVKAEKAEKVKKASEENGKVEEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLY 216
           V+K        K+ +E+G     +  WS+++Y  Y +R+ LP+N + +++KAE+K+GVLY
Sbjct: 122 VIKGG-----HKSEQEHG----GDDSWSSRTYSSYDTRLKLPDNCEKDKVKAELKNGVLY 181

Query: 217 ITIPKAMATSKIVDINVQ 233
           ITIPK     K++D+ VQ
Sbjct: 182 ITIPKTKVERKVIDVQVQ 181

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BLAST of Cucsat.G9573 vs. ExPASy Swiss-Prot
Match: P30222 (Small heat shock protein, chloroplastic OS=Petunia hybrida OX=4102 GN=HSP22 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.6e-28
Identity = 81/230 (35.22%), Postives = 127/230 (55.22%), Query Frame = 0


Query: 12  VCFTPTKASNDPKKFVRKSSVKAVRGDGRENLD-----HLQRTIE----KEKQTAQPPKK 71
           VCF  +K S      VRK + + V     +N D     H+    +    +     + P++
Sbjct: 37  VCFPYSKCS------VRKPASRLVAQATGDNKDTSVDVHVSNNNQGGNNQGSAVERRPRR 96

Query: 72  RVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSRGRTPW 131
               V+P GL D     RT++QMM+TM+R+ +D   + G        S   G    R PW
Sbjct: 97  MALDVSPFGLLDPMSPMRTMRQMMDTMDRLFEDTMTFPG--------SRNRGTGEIRAPW 156

Query: 132 EIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKVEEEEGDWS 191
           +IK+ ENE KMRFDMPG++K++VKV VE+ +LV+K E  +      EE+GK    +  W 
Sbjct: 157 DIKDDENEIKMRFDMPGLSKEEVKVSVEDDVLVIKGEHKK------EESGK----DDSW- 216

Query: 192 AKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 233
            ++Y  Y +R++LP+NV  +++KAE+K+GVL I+IPK     K+ D+ ++
Sbjct: 217 GRNYSSYDTRLSLPDNVDKDKVKAELKNGVLLISIPKTKVEKKVTDVEIK 241

[Close]

BLAST of Cucsat.G9573 vs. ExPASy Swiss-Prot
Match: Q95661 (Small heat shock protein, chloroplastic OS=Solanum lycopersicum OX=4081 GN=HSP21 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 4.4e-28
Identity = 68/172 (39.53%), Postives = 108/172 (62.79%), Query Frame = 0


Query: 60  PKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSRGR 119
           P +    V+P G+ D     RT++QM++TM+R+ +D   + G        +  +G    R
Sbjct: 80  PTRMALDVSPFGVLDPMSPMRTMRQMIDTMDRLFEDTMTFPG-------RNRASGTGEIR 139

Query: 120 TPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKVEEEEG 179
           TPW+I + ENE KMRFDMPG++K+DVKV VE  MLV+K E  +      EE+G+   ++ 
Sbjct: 140 TPWDIHDDENEIKMRFDMPGLSKEDVKVSVENDMLVIKGEHKK------EEDGR---DKH 199

Query: 180 DWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINV 232
            W  ++Y  Y +R++LP+NV  ++IKAE+K+GVL+I+IPK     K++D+ +
Sbjct: 200 SW-GRNYSSYDTRLSLPDNVVKDKIKAELKNGVLFISIPKTEVEKKVIDVQI 234

[Close]

BLAST of Cucsat.G9573 vs. NCBI nr
Match: XP_004135300.1 (small heat shock protein, chloroplastic [Cucumis sativus])

HSP 1 Score: 459 bits (1182), Expect = 4.98e-163
Identity = 232/232 (100.00%), Postives = 232/232 (100.00%), Query Frame = 0


Query: 1   MAQAVSNLTRFVCFTPTKASNDPKKFVRKSSVKAVRGDGRENLDHLQRTIEKEKQTAQPP 60
           MAQAVSNLTRFVCFTPTKASNDPKKFVRKSSVKAVRGDGRENLDHLQRTIEKEKQTAQPP
Sbjct: 1   MAQAVSNLTRFVCFTPTKASNDPKKFVRKSSVKAVRGDGRENLDHLQRTIEKEKQTAQPP 60

Query: 61  KKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSRGRT 120
           KKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSRGRT
Sbjct: 61  KKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSRGRT 120

Query: 121 PWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKVEEEEGD 180
           PWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKVEEEEGD
Sbjct: 121 PWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKVEEEEGD 180

Query: 181 WSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 232
           WSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ
Sbjct: 181 WSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 232

[Close]

BLAST of Cucsat.G9573 vs. NCBI nr
Match: XP_008446109.1 (PREDICTED: small heat shock protein, chloroplastic-like [Cucumis melo] >KAA0034232.1 small heat shock protein [Cucumis melo var. makuwa] >TYK15688.1 small heat shock protein [Cucumis melo var. makuwa])

HSP 1 Score: 425 bits (1092), Expect = 2.43e-149
Identity = 218/233 (93.56%), Postives = 224/233 (96.14%), Query Frame = 0


Query: 1   MAQAVSNLTRFVCFTPTKA-SNDPKKFVRKSSVKAVRGDGRENLDHLQRTIEKEKQTAQP 60
           MAQAVSNLTRFV FTPTKA SNDPKKFVR+S+VKAV GDGR+NLDHLQRTI KEKQT QP
Sbjct: 1   MAQAVSNLTRFVSFTPTKAASNDPKKFVRRSNVKAVAGDGRDNLDHLQRTIGKEKQTTQP 60

Query: 61  PKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSRGR 120
           PKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTS+GAGYSRGR
Sbjct: 61  PKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSEGAGYSRGR 120

Query: 121 TPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKVEEEEG 180
           TPWEIKEGENEYKMRFDMPGMTK+DVKVWVEEKMLVVKAEK   VKKA EENGKVEEEEG
Sbjct: 121 TPWEIKEGENEYKMRFDMPGMTKNDVKVWVEEKMLVVKAEK---VKKAGEENGKVEEEEG 180

Query: 181 DWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 232
           DWSAKSYG+YSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ
Sbjct: 181 DWSAKSYGKYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 230

[Close]

BLAST of Cucsat.G9573 vs. NCBI nr
Match: XP_038893285.1 (small heat shock protein, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 403 bits (1036), Expect = 9.29e-141
Identity = 208/236 (88.14%), Postives = 218/236 (92.37%), Query Frame = 0


Query: 1   MAQAVSNLTRFVCFTPTKAS-NDPKKFV---RKSSVKAVRGDGRENLDHLQRTIEKEKQT 60
           MAQAVSNL RFV FTP+KAS NDPKK     R+SS+KA+ G+G++NLDHLQR + KE QT
Sbjct: 1   MAQAVSNLPRFVSFTPSKASSNDPKKLTMLARRSSIKAMAGNGKDNLDHLQRAVGKEHQT 60

Query: 61  AQPPKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYS 120
           +QPPKKRVAPV   GLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYS
Sbjct: 61  SQPPKKRVAPV---GLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYS 120

Query: 121 RGRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKVEE 180
           RGRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKAS ENGKVEE
Sbjct: 121 RGRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASSENGKVEE 180

Query: 181 EEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 232
           EEGDWSAKSYGRYSSRIALPEN+QFEQIKAEVKDGVLYITIPKA  TSKIVDINVQ
Sbjct: 181 EEGDWSAKSYGRYSSRIALPENIQFEQIKAEVKDGVLYITIPKASVTSKIVDINVQ 233

[Close]

BLAST of Cucsat.G9573 vs. NCBI nr
Match: XP_022956582.1 (small heat shock protein, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 399 bits (1024), Expect = 6.97e-139
Identity = 205/239 (85.77%), Postives = 216/239 (90.38%), Query Frame = 0


Query: 1   MAQAVSNLTRFVCFTPTKASNDPKK---FVRKSSVKAVRGDGRENLDHLQRTIEKEKQTA 60
           MAQAVSNLTRFV FTP + SNDPKK   F R+S +KA+ GDGR+NLDHLQ+   KE++T 
Sbjct: 1   MAQAVSNLTRFVSFTPGRVSNDPKKLKGFARRSCIKAMAGDGRDNLDHLQKISRKEQRTT 60

Query: 61  QPPKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSR 120
           QPPKKRVAPVAP+GLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPF+SDGAGYSR
Sbjct: 61  QPPKKRVAPVAPVGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFSSDGAGYSR 120

Query: 121 GRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKV--- 180
           GRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEK   VKK S ENGKV   
Sbjct: 121 GRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEK---VKKTSSENGKVGGE 180

Query: 181 -EEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 232
            EEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKA+ TSKIVDINVQ
Sbjct: 181 EEEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAVVTSKIVDINVQ 236

[Close]

BLAST of Cucsat.G9573 vs. NCBI nr
Match: KAG7032337.1 (25.3 kDa heat shock protein, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 398 bits (1023), Expect = 9.90e-139
Identity = 205/239 (85.77%), Postives = 216/239 (90.38%), Query Frame = 0


Query: 1   MAQAVSNLTRFVCFTPTKASNDPKK---FVRKSSVKAVRGDGRENLDHLQRTIEKEKQTA 60
           MAQAVSNLTRFV FTP + SNDPKK   F R+S +KA+ GDGR+NLDHLQ+   KE++T 
Sbjct: 1   MAQAVSNLTRFVSFTPGRVSNDPKKLKGFARRSCIKAMAGDGRDNLDHLQKIPRKEQRTT 60

Query: 61  QPPKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSR 120
           QPPKKRVAPVAP+GLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPF+SDGAGYSR
Sbjct: 61  QPPKKRVAPVAPVGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFSSDGAGYSR 120

Query: 121 GRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKV--- 180
           GRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEK   VKK S ENGKV   
Sbjct: 121 GRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEK---VKKTSSENGKVGGG 180

Query: 181 -EEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 232
            EEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKA+ TSKIVDINVQ
Sbjct: 181 EEEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAVVTSKIVDINVQ 236

[Close]

BLAST of Cucsat.G9573 vs. ExPASy TrEMBL
Match: A0A0A0KTF5 (SHSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G591720 PE=3 SV=1)

HSP 1 Score: 459 bits (1182), Expect = 2.41e-163
Identity = 232/232 (100.00%), Postives = 232/232 (100.00%), Query Frame = 0


Query: 1   MAQAVSNLTRFVCFTPTKASNDPKKFVRKSSVKAVRGDGRENLDHLQRTIEKEKQTAQPP 60
           MAQAVSNLTRFVCFTPTKASNDPKKFVRKSSVKAVRGDGRENLDHLQRTIEKEKQTAQPP
Sbjct: 1   MAQAVSNLTRFVCFTPTKASNDPKKFVRKSSVKAVRGDGRENLDHLQRTIEKEKQTAQPP 60

Query: 61  KKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSRGRT 120
           KKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSRGRT
Sbjct: 61  KKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSRGRT 120

Query: 121 PWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKVEEEEGD 180
           PWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKVEEEEGD
Sbjct: 121 PWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKVEEEEGD 180

Query: 181 WSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 232
           WSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ
Sbjct: 181 WSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 232

[Close]

BLAST of Cucsat.G9573 vs. ExPASy TrEMBL
Match: A0A5A7ST00 (Small heat shock protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001860 PE=3 SV=1)

HSP 1 Score: 425 bits (1092), Expect = 1.18e-149
Identity = 218/233 (93.56%), Postives = 224/233 (96.14%), Query Frame = 0


Query: 1   MAQAVSNLTRFVCFTPTKA-SNDPKKFVRKSSVKAVRGDGRENLDHLQRTIEKEKQTAQP 60
           MAQAVSNLTRFV FTPTKA SNDPKKFVR+S+VKAV GDGR+NLDHLQRTI KEKQT QP
Sbjct: 1   MAQAVSNLTRFVSFTPTKAASNDPKKFVRRSNVKAVAGDGRDNLDHLQRTIGKEKQTTQP 60

Query: 61  PKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSRGR 120
           PKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTS+GAGYSRGR
Sbjct: 61  PKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSEGAGYSRGR 120

Query: 121 TPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKVEEEEG 180
           TPWEIKEGENEYKMRFDMPGMTK+DVKVWVEEKMLVVKAEK   VKKA EENGKVEEEEG
Sbjct: 121 TPWEIKEGENEYKMRFDMPGMTKNDVKVWVEEKMLVVKAEK---VKKAGEENGKVEEEEG 180

Query: 181 DWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 232
           DWSAKSYG+YSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ
Sbjct: 181 DWSAKSYGKYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 230

[Close]

BLAST of Cucsat.G9573 vs. ExPASy TrEMBL
Match: A0A1S3BEA1 (small heat shock protein, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103488933 PE=3 SV=1)

HSP 1 Score: 425 bits (1092), Expect = 1.18e-149
Identity = 218/233 (93.56%), Postives = 224/233 (96.14%), Query Frame = 0


Query: 1   MAQAVSNLTRFVCFTPTKA-SNDPKKFVRKSSVKAVRGDGRENLDHLQRTIEKEKQTAQP 60
           MAQAVSNLTRFV FTPTKA SNDPKKFVR+S+VKAV GDGR+NLDHLQRTI KEKQT QP
Sbjct: 1   MAQAVSNLTRFVSFTPTKAASNDPKKFVRRSNVKAVAGDGRDNLDHLQRTIGKEKQTTQP 60

Query: 61  PKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSRGR 120
           PKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTS+GAGYSRGR
Sbjct: 61  PKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSEGAGYSRGR 120

Query: 121 TPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKVEEEEG 180
           TPWEIKEGENEYKMRFDMPGMTK+DVKVWVEEKMLVVKAEK   VKKA EENGKVEEEEG
Sbjct: 121 TPWEIKEGENEYKMRFDMPGMTKNDVKVWVEEKMLVVKAEK---VKKAGEENGKVEEEEG 180

Query: 181 DWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 232
           DWSAKSYG+YSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ
Sbjct: 181 DWSAKSYGKYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 230

[Close]

BLAST of Cucsat.G9573 vs. ExPASy TrEMBL
Match: A0A6J1GWR4 (small heat shock protein, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458276 PE=3 SV=1)

HSP 1 Score: 399 bits (1024), Expect = 3.37e-139
Identity = 205/239 (85.77%), Postives = 216/239 (90.38%), Query Frame = 0


Query: 1   MAQAVSNLTRFVCFTPTKASNDPKK---FVRKSSVKAVRGDGRENLDHLQRTIEKEKQTA 60
           MAQAVSNLTRFV FTP + SNDPKK   F R+S +KA+ GDGR+NLDHLQ+   KE++T 
Sbjct: 1   MAQAVSNLTRFVSFTPGRVSNDPKKLKGFARRSCIKAMAGDGRDNLDHLQKISRKEQRTT 60

Query: 61  QPPKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSR 120
           QPPKKRVAPVAP+GLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPF+SDGAGYSR
Sbjct: 61  QPPKKRVAPVAPVGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFSSDGAGYSR 120

Query: 121 GRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKV--- 180
           GRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEK   VKK S ENGKV   
Sbjct: 121 GRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEK---VKKTSSENGKVGGE 180

Query: 181 -EEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 232
            EEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKA+ TSKIVDINVQ
Sbjct: 181 EEEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAVVTSKIVDINVQ 236

[Close]

BLAST of Cucsat.G9573 vs. ExPASy TrEMBL
Match: A0A6J1KH52 (small heat shock protein, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111493168 PE=3 SV=1)

HSP 1 Score: 394 bits (1012), Expect = 2.19e-137
Identity = 203/238 (85.29%), Postives = 213/238 (89.50%), Query Frame = 0


Query: 1   MAQAVSNLTRFVCFTPTKASNDPKK---FVRKSSVKAVRGDGRENLDHLQRTIEKEKQTA 60
           MAQAVSNLTR V FTP K SNDPKK   F R+S +KA+ GDGR+NLDHLQ+   KE++  
Sbjct: 1   MAQAVSNLTRLVSFTPGKVSNDPKKLKGFARRSCIKAMAGDGRDNLDHLQKIPRKEQRAT 60

Query: 61  QPPKKRVAPVAPIGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFTSDGAGYSR 120
           QPPKKRVAPV P+GLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPF+SDGAGYSR
Sbjct: 61  QPPKKRVAPVVPVGLWDRFPTARTVQQMMETMERIMDDPFAYSGAWPSPPFSSDGAGYSR 120

Query: 121 GRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEKAEKVKKASEENGKV--- 180
           GRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEK   VKK S ENGKV   
Sbjct: 121 GRTPWEIKEGENEYKMRFDMPGMTKDDVKVWVEEKMLVVKAEK---VKKTSSENGKVGEE 180

Query: 181 EEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAMATSKIVDINVQ 232
           EEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKA+ TSKIVDINVQ
Sbjct: 181 EEEEGDWSAKSYGRYSSRIALPENVQFEQIKAEVKDGVLYITIPKAVVTSKIVDINVQ 235

The following BLAST results are available for this feature:

BLAST of Cucsat.G9573 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P31170	7.8e-33	38.68	Heat shock protein 21, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HSP21 PE...	[more]
P09886	2.5e-31	39.39	Small heat shock protein, chloroplastic OS=Pisum sativum OX=3888 GN=HSP21 PE=2 S...	[more]
P09887	1.6e-30	35.86	Small heat shock protein, chloroplastic (Fragment) OS=Glycine max OX=3847 GN=HSP...	[more]
P30222	2.6e-28	35.22	Small heat shock protein, chloroplastic OS=Petunia hybrida OX=4102 GN=HSP22 PE=2...	[more]
Q95661	4.4e-28	39.53	Small heat shock protein, chloroplastic OS=Solanum lycopersicum OX=4081 GN=HSP21...	[more]

BLAST of Cucsat.G9573 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135300.1	4.98e-163	100.00	small heat shock protein, chloroplastic [Cucumis sativus]	[more]
XP_008446109.1	2.43e-149	93.56	PREDICTED: small heat shock protein, chloroplastic-like [Cucumis melo] >KAA00342...	[more]
XP_038893285.1	9.29e-141	88.14	small heat shock protein, chloroplastic-like [Benincasa hispida]	[more]
XP_022956582.1	6.97e-139	85.77	small heat shock protein, chloroplastic-like [Cucurbita moschata]	[more]
KAG7032337.1	9.90e-139	85.77	25.3 kDa heat shock protein, chloroplastic [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Cucsat.G9573 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KTF5	2.41e-163	100.00	SHSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G591720 PE=3 S...	[more]
A0A5A7ST00	1.18e-149	93.56	Small heat shock protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3BEA1	1.18e-149	93.56	small heat shock protein, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A6J1GWR4	3.37e-139	85.77	small heat shock protein, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1KH52	2.19e-137	85.29	small heat shock protein, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 157..178
None	No IPR available	PANTHER	PTHR46733:SF2	26.5 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL	coord: 1..232
None	No IPR available	CDD	cd06464	ACD_sHsps-like	coord: 123..219 e-value: 3.62686E-19 score: 77.2092
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 123..229 e-value: 1.9E-22 score: 79.3
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 113..232 score: 24.713577
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 77..231 e-value: 1.1E-29 score: 105.1
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 86..223
IPR044587	Heat shock protein 21-like	PANTHER	PTHR46733	26.5 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL	coord: 1..232

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G9573.T1	Cucsat.G9573.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009408	response to heat