Cucsat.G9170 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G9170
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	hemK methyltransferase family member 2
Location	ctg1658: 814604 .. 818287 (+)
RNA-Seq Expression	Cucsat.G9170
Synteny	Cucsat.G9170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTAAAAGTAATGTTGAAAATTGAATAGTATACTTTAGTTGGAGGTTGAAACGCTCCGCCCCAATAGGCAATAGCTTCTTGAAAAACCCAATTTCCTTACCCATCACTCTCCACTCTCCCGCAGCCCAACGTATCTTCTCCTTCCCCATTCTGTATCTCTTTGCCTTTCCAACGCTCAATCTTCCTCAATACTTCTCCTTACTTTCCATCTCCGACCTTTCATAACTCTCTTCAGTCTTTCAGTTGCTTTCTCATTTTCTCACCGCACTTTTCTCACCGCACTTTTCTCCTTTCCTGGTATTCTTTCCGTTTCTTTATCTTTCTTTCTAATTCATTTTATCTCTTTCCATTGTGATTGATTTCGAGCTTTATCGCTGAAGTCCACCAACTTCTTTTTTCTCTCTCTTTTTCCCACCCGAAATGAAATTTGTTTGTGTACAGTTTTATGTGGCTGCTGCTTTTTGCTCATGTTCTGGAATCCGAATTGAGTCGTTTGTGGTTCATGTTTTTTTTTTAAAAAAAATTGAATCAAGGGGTCTTTTGTTGTGATTGGTTTTCTTTAATTTTGGCTCCGTTTTACTTCTTGACTTGTTTTATCGGTCTCTTGTTCTTTTGAAATTGAGAAATCGTTGCCCCCATTGCTGATTGAAGTGAAGGTTTTGATCCCACCCTGTAGGAACGTGTTGTACTAGCATGAAATTGTGGGAATTGCTTGATGATTGCCCGGGAACTGTCTGAGGAAAGAACTATTGAGATTTGTAGAACTCAGAAGTTGTGATTGGTTTTTTCAAGAAAAAATTTAATGGTTCTTATAGATTAACTGTTTCATTAATCAGCAGATTTAGTTCTGATAATTGATCCGCTTCTCCCCATCCTTCTTAGATTAGAGCGAGAGTACACTCACCTATCTCCCTTGCTTGGATTCGGGTTTCACTTCCTGCATCACTTTTTTGGTGGTGTTTTCTTTTTGGAAACAACTGTTTCTTATAGTGCACATACAGACCAGCACGCGTATTAGTAGACAATTGTCTTATTTTTCATATTTTCATGACAGGTCCGGCACATGTCCTCTTTCAGAACTGCACAAATTCCCCTAGTGAGCTCTCATCGTGAGGTTTATGAACCCTGTGATGATTCGTTTGCTTTGGTTGATGCACTGTTAGCTGATCAAGTTAAATTGTTAAATCATCATCCAACATTGTGCTTGGAATTTGGTTGTGGTAGTGGCTATGTTATAACATCGCTTGCTCTTATTCTGGGAAAGGAAGCTTCTGCAACCCATTATATTGCTACAGACATCAATCCTCATGCAATTAGAGTCACTCAGGAGACTCTAGAAGCACATGGGCTTCATGCAGAGTTGGTATGCACTGACATTGCATCTGGTTTGGAGAAACGACTGGCTGGATCAGTTGATGTGTTGGTTGTGAATCCACCCTATGTTCCAACCCCAGAGGACGAAGTGGGTCAGGATGGAATTGCTTCTGCTTGGGCGGGAGGGGAAAATGGTCGCAGTGTCATTGACAGGATATTGCCTGTTGCTGATGTTCTTCTGTCTGATAAAGGCTGGTTGTACATGGTTACTCTTACAGCAAATAATCCCGCTCAGATATGCCTTCAAATGAGAGAAAAAGGATATGCTAGTAGAATTGTTGTGCAGAGATCAACTGAAGAAGAGAGCCTTCACGTGATAAAGTTTTGGAAGGATGCTGATCTCCAAGTTGATGGAAAAGATTCAACACATAAAACAGGGCCTGGAAAGGTTGTCGAGTCTCTAATTTCACAACTTCCTCGTCTCTCGTTTTGGAGAAACAACAGCAACACTAGCAGATGAATGAGTTATCAGAGTACGTTTCAAGACCTCATGTCTTCTCTAATAATTCATCGTTTTTATTTTAATTTTCTGCCGCTTTTCTGCTCTTCCTTATACACTCGACCAAGTAGCTGTTACTGAAAGTCTCATTTTAGTGAAAGCAATAAGTAATCATCCCTTAGTTTGGATTTCACTCCGATTACTATGCCATCTTTCTTCGAAATCCTGTCCCATTATCAATGTTTCTCCTCACTGCCCATCTACGCTAACTAATGAAGATCGAATTCAATGGTGTGAATCTAATTACCATGTCTAGGTTACCGCATTCTTCCTTGTTTTAATTTTTCTTCATATTGTCTAACCACGCTTGGAAGATTTCTCCACAAAACTGAGCTTATATTTACGCTCCATTTGGATTATATGTTTTTTTAAAAATAAGCTTTTAAATATAGTTCTTTGACCAATGAATTTCTCTGCTTTTCGATTCACTTTCTACTCATTGTGTTGAAAACCAAGCCAATTTTAAAATATTGTTTTTAAAAACTTATTTTTGCTTTTGGAATTTAACTAAGATGTTTGGGGTGTTGAGTTGGTTGTTATAGTTAGTAGTTATTATAATATGTGAGTTATAATATTTTGTATTTGGTGGCAAGCTAATTATAGCTTGGGTAGAAAATAGTAAACAATCTAACAAAGGTCAAAGATAGAATGGGCAGGAGAATAGTAAGCCTTATAAGAGAGAGAGATTTGAAATTGTAATCTTGTAGTTAATGGTAGATTATAATAGTTGGATTAACCGACTATTATAATTAAATAGGATCCCCAAACGTAATTAGCCCACTACACCCACTTGAATTGGGAGTCCAAACCCCCCTAAATGTCTCTTTAATAAAGATGAAAATCTTAGTATGGAAATAAGGTGACAAGCATAATTAAGGTTAAGTGGTTATCAAATGGGGTTGCAGTGATACAATTTCAATCCCTAAACATCATTGGGATGACCTGGTGGTCAATAGAGGCCATGGGTTTATCAAATGACTCACCTATCTAAAATTTTTTTGATACCTAAATATTGTAGGATTAAATAAGTTCTCCCAGTGAGATTACTTAAGGTGTGGGCAAGCAGGCTTGAGCTCTTGCAGTTATGTTGAAGAAAAAGAAAATTAACCTCGTGATTCCACACAATTTACAGCCTAAACATACACAGCCGGCATCGTTTAAAACTAGTAGAGTAGATACATGCATGCCTTTGCTTTGTCTTTAATATTAGTTAACGATATTGAAACTTGAATGTACTATAGACTAATTTGAGTAGCCATCTTCTTGGTCGTGCCTCTCTTGCAGGACTTGAGACTCATCCATACATCCTTCATTGTTACACAAGCAACAAGTCCAACCTTTCAACTCTTGTGCATTCCACAGGTGCATAGTCTTCGTTTTTGTAAATGCAAATACCTATTTTTTGACCCTAAAACTTCTTAGAGATTTGTCGTAGTTAGTCATTAACTGTCACCTGCTGAGCTTGAAAGTTCAAGTTCTCTTAGATCATAGGAATCGCTGTTTCATTAACAAGATATCACTGAAGATCTATGAATTGGGCAGCTGCAGTTGTGTTGCAACAATCTGTCTGAATGTTTTTGATGTACCACATTTGTTCTCATCTGATGATTTAGTTTTGTCACTATTTTCTTCTAGTTCTATATGTGTTCCTTAATCCTTTTGGAGGTTGAATTTCATTTGGCTATTTCATTTCTTTTTTTGTAAGCATCATACTGGCTTGATCCTTTGCTTTCAGTAAGCTTATGGTGTGTTCGAGAATGATTTGAAATAGTTAATTGACATTTGTTTTAAGTAACAATATTATCAATTGT

Coding sequence (CDS)

ATGTCCTCTTTCAGAACTGCACAAATTCCCCTAGTGAGCTCTCATCGTGAGGTTTATGAACCCTGTGATGATTCGTTTGCTTTGGTTGATGCACTGTTAGCTGATCAAGTTAAATTGTTAAATCATCATCCAACATTGTGCTTGGAATTTGGTTGTGGTAGTGGCTATGTTATAACATCGCTTGCTCTTATTCTGGGAAAGGAAGCTTCTGCAACCCATTATATTGCTACAGACATCAATCCTCATGCAATTAGAGTCACTCAGGAGACTCTAGAAGCACATGGGCTTCATGCAGAGTTGGTATGCACTGACATTGCATCTGGTTTGGAGAAACGACTGGCTGGATCAGTTGATGTGTTGGTTGTGAATCCACCCTATGTTCCAACCCCAGAGGACGAAGTGGGTCAGGATGGAATTGCTTCTGCTTGGGCGGGAGGGGAAAATGGTCGCAGTGTCATTGACAGGATATTGCCTGTTGCTGATGTTCTTCTGTCTGATAAAGGCTGGTTGTACATGGTTACTCTTACAGCAAATAATCCCGCTCAGATATGCCTTCAAATGAGAGAAAAAGGATATGCTAGTAGAATTGTTGTGCAGAGATCAACTGAAGAAGAGAGCCTTCACGTGATAAAGTTTTGGAAGGATGCTGATCTCCAAGTTGATGGAAAAGATTCAACACATAAAACAGGGCCTGGAAAGGTTGTCGAGTCTCTAATTTCACAACTTCCTCGTCTCTCGTTTTGGAGAAACAACAGCAACACTAGCAGATGA

Protein sequence

MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITSLALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVLVVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNPAQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLISQLPRLSFWRNNSNTSR

Homology

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BLAST of Cucsat.G9170 vs. ExPASy Swiss-Prot
Match: Q6SKR2 (Methyltransferase N6AMT1 OS=Mus musculus OX=10090 GN=N6amt1 PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 3.5e-42
Identity = 92/199 (46.23%), Postives = 121/199 (60.80%), Query Frame = 0


Query: 16  REVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITSLALILGKEASATHYI 75
           R+VYEP +D+F L+DAL A   +L      +CLE G GSG V   LA ++G  A    Y+
Sbjct: 20  RDVYEPAEDTFLLLDALEAAAAELAG--VEICLEVGAGSGVVSAFLASMIGPRAL---YM 79

Query: 76  ATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVLVVNPPYVPTPEDEVG 135
            TDINP A   T ET   + +H + V TD+  GL  RL G VD+LV NPPYV TP +EVG
Sbjct: 80  CTDINPEAAACTLETARCNRVHVQPVITDLVHGLLPRLKGKVDLLVFNPPYVVTPPEEVG 139

Query: 136 QDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNPAQICLQMREKGYASR 195
             GI +AWAGG NGR V+DR  P+A  LLS +G  Y+VT+  NNP +I   M+ +G    
Sbjct: 140 SRGIEAAWAGGRNGREVMDRFFPLAPELLSPRGLFYLVTVKENNPEEIFKTMKTRGLQGT 199

Query: 196 IVVQRSTEEESLHVIKFWK 215
             + R   +E+L V++F K
Sbjct: 200 TALCRQAGQEALSVLRFSK 213

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BLAST of Cucsat.G9170 vs. ExPASy Swiss-Prot
Match: Q9Y5N5 (Methyltransferase N6AMT1 OS=Homo sapiens OX=9606 GN=N6AMT1 PE=1 SV=4)

HSP 1 Score: 170.2 bits (430), Expect = 2.9e-41
Identity = 91/198 (45.96%), Postives = 118/198 (59.60%), Query Frame = 0


Query: 17  EVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITSLALILGKEASATHYIA 76
           +VYEP +D+F L+DAL A   +L      +CLE G GSG V   LA ++G +A    Y+ 
Sbjct: 21  DVYEPAEDTFLLLDALEAAAAELAG--VEICLEVGSGSGVVSAFLASMIGPQAL---YMC 80

Query: 77  TDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVLVVNPPYVPTPEDEVGQ 136
           TDINP A   T ET   + +H + V TD+  GL  RL   VD+LV NPPYV TP  EVG 
Sbjct: 81  TDINPEAAACTLETARCNKVHIQPVITDLVKGLLPRLTEKVDLLVFNPPYVVTPPQEVGS 140

Query: 137 DGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNPAQICLQMREKGYASRI 196
            GI +AWAGG NGR V+DR  P+   LLS +G  Y+VT+  NNP +I   M+ KG     
Sbjct: 141 HGIEAAWAGGRNGREVMDRFFPLVPDLLSPRGLFYLVTIKENNPEEILKIMKTKGLQGTT 200

Query: 197 VVQRSTEEESLHVIKFWK 215
            + R   +E+L V+KF K
Sbjct: 201 ALSRQAGQETLSVLKFTK 213

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BLAST of Cucsat.G9170 vs. ExPASy Swiss-Prot
Match: Q9UT94 (eRF1 methyltransferase catalytic subunit mtq2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mtq2 PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.8e-22
Identity = 74/221 (33.48%), Postives = 113/221 (51.13%), Query Frame = 0


Query: 6   TAQIPLVSSHREVYEPCDDSFALVDALLADQVKL---LNHHPTLCLEFGCGSGYVITSLA 65
           T+Q+ L    ++VYEP +D+FAL+DAL  D  KL         L  E GCGSG   + L 
Sbjct: 7   TSQLRL-KEFQDVYEPAEDTFALLDALEKDAKKLRQMAEMKNLLTAEIGCGSGCASSFLK 66

Query: 66  LILGKEASATHYIATDINPHAIRVTQET-LEAHGLHAE------LVCTDIASGLEKRLAG 125
             + K     H++ +DI+  A R ++ T L    L+ +       V T    G+  RL  
Sbjct: 67  SGILKNKPIVHFM-SDISNSACRASKITALNNRELYKDDNGLFITVQTSFLDGI--RLGN 126

Query: 126 SVDVLVVNPPYVPTPEDEVGQDG--IASAWAGGENGRSVIDRILPVADVLLSDKGWLYMV 185
            VD+L+ NPPYVPT  +E+  +   IASAWAGG +G  V   +L     +LS  G  YMV
Sbjct: 127 GVDILIFNPPYVPTEFEEIPSEAATIASAWAGGTDGMDVTSTLLNQLKDILSQDGVFYMV 186

Query: 186 TLTANNPAQICLQMREKGYASRIVVQRSTEEESLHVIKFWK 215
            +  N    IC  +++ G+     ++R    E+L +++ ++
Sbjct: 187 AVARNKLHSICEILQKDGFIVNETLKRKAGRETLSILRIYR 223

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BLAST of Cucsat.G9170 vs. ExPASy Swiss-Prot
Match: Q58338 (Putative protein N5-glutamine methyltransferase MJ0928 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0928 PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 5.6e-16
Identity = 54/200 (27.00%), Postives = 96/200 (48.00%), Query Frame = 0


Query: 15  HREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITSLALILGKEASATHY 74
           H EVYEP +DS  L+  L+  + K +       LE G G+G +  + A     +  A   
Sbjct: 13  HPEVYEPAEDSILLLKNLVDVKNKDV-------LEIGVGTGLISIACA-----KKGAKKI 72

Query: 75  IATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVLVVNPPYVPTPEDEV 134
           +  DINP+A+++ +E  + + ++         S L + + G  DV++ NPPY+PT EDE 
Sbjct: 73  VGVDINPYAVKLAKENAKLNNVNISF----FESDLFENVTGKFDVILFNPPYLPTSEDEK 132

Query: 135 GQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNPAQICLQMREKGYAS 194
               +  A+ GG++GR ++DR +      L   G + ++  +     +   +++  G+  
Sbjct: 133 IDSYLNFAFDGGKDGREILDRFIYELPNYLKKGGVVQILQSSLTGEKETINKLKPLGFKV 192

Query: 195 RIVVQRSTEEESLHVIKFWK 215
            I  +     E L VI  W+
Sbjct: 193 EISARLKVPFEELMVINAWR 196

[Close]

BLAST of Cucsat.G9170 vs. ExPASy Swiss-Prot
Match: O28490 (Putative protein N5-glutamine methyltransferase AF_1784 OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=AF_1784 PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 8.9e-14
Identity = 57/198 (28.79%), Postives = 94/198 (47.47%), Query Frame = 0


Query: 18  VYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITSLALILGKEASATHYIAT 77
           +YEP +DS  L++A L +            +E G GSG+V   L      +      + T
Sbjct: 2   IYEPAEDSELLLEAALREL-----KPDDEVIEVGAGSGFVAERL------KGKCKCILTT 61

Query: 78  DINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVLVVNPPYVPTPEDEVGQD 137
           DI+P A       +E      ++V TDIA G+ K+ +    +++ NPPYV   ++    D
Sbjct: 62  DISPFA------AMELRRKGLDVVMTDIAKGIRKKFS----LVLFNPPYVELEDELRRGD 121

Query: 138 GIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNPAQICLQMREKGYASRIV 197
            +  A  GG+ G  VI + L   D +++++G   ++  + N P    L + E+G+   IV
Sbjct: 122 WLDVAIDGGKKGVKVIKKFLDSLDEIMAERGRAILIASSQNEPDVFDL-IDERGFRYEIV 177

Query: 198 VQRSTEEESLHVIKFWKD 216
            +R    E L  IK WK+
Sbjct: 182 GERGLFFEKLFAIKIWKE 177

[Close]

BLAST of Cucsat.G9170 vs. NCBI nr
Match: XP_004136904.1 (methyltransferase N6AMT1 [Cucumis sativus] >XP_011658842.1 methyltransferase N6AMT1 [Cucumis sativus] >KGN43791.1 hypothetical protein Csa_017126 [Cucumis sativus])

HSP 1 Score: 521 bits (1343), Expect = 9.00e-187
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0


Query: 1   MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS 60
           MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS
Sbjct: 1   MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS 60

Query: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL 120
           LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLIS 240
           AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLIS
Sbjct: 181 AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLIS 240

Query: 241 QLPRLSFWRNNSNTSR 256
           QLPRLSFWRNNSNTSR
Sbjct: 241 QLPRLSFWRNNSNTSR 256

[Close]

BLAST of Cucsat.G9170 vs. NCBI nr
Match: XP_008455111.1 (PREDICTED: hemK methyltransferase family member 2 [Cucumis melo] >ADN34260.1 N6-DNA-methyltransferase [Cucumis melo subsp. melo] >KAA0031420.1 hemK methyltransferase family member 2 [Cucumis melo var. makuwa])

HSP 1 Score: 508 bits (1309), Expect = 1.38e-181
Identity = 249/256 (97.27%), Postives = 254/256 (99.22%), Query Frame = 0


Query: 1   MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS 60
           MSSFRTAQI LVSSHREVYEPCDDSFALVDALLAD+VKLLNH+PTLCLEFGCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHNPTLCLEFGCGSGYVITS 60

Query: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL 120
           LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTD+ASGLEKRLAGSVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDLASGLEKRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLIS 240
            QICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPG+VVESLIS
Sbjct: 181 FQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240

Query: 241 QLPRLSFWRNNSNTSR 256
           QLPRLSFWRNNSNT+R
Sbjct: 241 QLPRLSFWRNNSNTNR 256

[Close]

BLAST of Cucsat.G9170 vs. NCBI nr
Match: TYK06871.1 (hemK methyltransferase family member 2 [Cucumis melo var. makuwa])

HSP 1 Score: 506 bits (1303), Expect = 1.13e-180
Identity = 248/256 (96.88%), Postives = 253/256 (98.83%), Query Frame = 0


Query: 1   MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS 60
           MSSFR AQI LVSSHREVYEPCDDSFALVDALLAD+VKLLNH+PTLCLEFGCGSGYVITS
Sbjct: 1   MSSFRIAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHNPTLCLEFGCGSGYVITS 60

Query: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL 120
           LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTD+ASGLEKRLAGSVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDLASGLEKRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLIS 240
            QICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPG+VVESLIS
Sbjct: 181 FQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240

Query: 241 QLPRLSFWRNNSNTSR 256
           QLPRLSFWRNNSNT+R
Sbjct: 241 QLPRLSFWRNNSNTNR 256

[Close]

BLAST of Cucsat.G9170 vs. NCBI nr
Match: XP_038888861.1 (methyltransferase N6AMT1 [Benincasa hispida])

HSP 1 Score: 496 bits (1278), Expect = 7.32e-177
Identity = 242/256 (94.53%), Postives = 251/256 (98.05%), Query Frame = 0


Query: 1   MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS 60
           MSSFR AQI LVSSHREVYEPCDDSFALVDALLAD+VKLLNHHPTLCLE GCGSGYVITS
Sbjct: 1   MSSFRIAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHHPTLCLEVGCGSGYVITS 60

Query: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL 120
           LALILGKEAS THYIATDINPHAIRVTQETLEAHG+HAELV TDIASGLE+RLAGSVDVL
Sbjct: 61  LALILGKEASGTHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEQRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVG+DGIASAWAGGENGRSVIDRILPVADVLLS+KGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILPVADVLLSEKGWLYMVTLTANNP 180

Query: 181 AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLIS 240
           +QICLQMREKGYASRI+VQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPG+VVESLIS
Sbjct: 181 SQICLQMREKGYASRIIVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240

Query: 241 QLPRLSFWRNNSNTSR 256
           QLPRLSFWRNNSNT+R
Sbjct: 241 QLPRLSFWRNNSNTNR 256

[Close]

BLAST of Cucsat.G9170 vs. NCBI nr
Match: XP_023553679.1 (hemK methyltransferase family member 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 490 bits (1262), Expect = 2.01e-174
Identity = 239/256 (93.36%), Postives = 248/256 (96.88%), Query Frame = 0


Query: 1   MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS 60
           MSSFRTAQI LVSSHREVYEPCDDSFALVDALLA++V LLNHHPTLCLE GCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLAERVNLLNHHPTLCLEVGCGSGYVITS 60

Query: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL 120
           LAL+LGKEAS  HYIATDINPHAIRVTQETLEAHG+HAELV TDIASGLEKRLAGSVDVL
Sbjct: 61  LALMLGKEASGAHYIATDINPHAIRVTQETLEAHGVHAELVSTDIASGLEKRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVG+DGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGRDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLIS 240
           +QICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGK+STHKTGP +VVESLIS
Sbjct: 181 SQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKESTHKTGPARVVESLIS 240

Query: 241 QLPRLSFWRNNSNTSR 256
           Q PRLSFWRNNSNT+R
Sbjct: 241 QFPRLSFWRNNSNTNR 256

[Close]

BLAST of Cucsat.G9170 vs. ExPASy TrEMBL
Match: A0A0A0K7J9 (MTS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067460 PE=3 SV=1)

HSP 1 Score: 521 bits (1343), Expect = 4.36e-187
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0


Query: 1   MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS 60
           MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS
Sbjct: 1   MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS 60

Query: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL 120
           LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLIS 240
           AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLIS
Sbjct: 181 AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLIS 240

Query: 241 QLPRLSFWRNNSNTSR 256
           QLPRLSFWRNNSNTSR
Sbjct: 241 QLPRLSFWRNNSNTSR 256

[Close]

BLAST of Cucsat.G9170 vs. ExPASy TrEMBL
Match: A0A5A7SL12 (HemK methyltransferase family member 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G001760 PE=3 SV=1)

HSP 1 Score: 508 bits (1309), Expect = 6.66e-182
Identity = 249/256 (97.27%), Postives = 254/256 (99.22%), Query Frame = 0


Query: 1   MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS 60
           MSSFRTAQI LVSSHREVYEPCDDSFALVDALLAD+VKLLNH+PTLCLEFGCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHNPTLCLEFGCGSGYVITS 60

Query: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL 120
           LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTD+ASGLEKRLAGSVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDLASGLEKRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLIS 240
            QICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPG+VVESLIS
Sbjct: 181 FQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240

Query: 241 QLPRLSFWRNNSNTSR 256
           QLPRLSFWRNNSNT+R
Sbjct: 241 QLPRLSFWRNNSNTNR 256

[Close]

BLAST of Cucsat.G9170 vs. ExPASy TrEMBL
Match: E5GCR1 (N6-DNA-methyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 508 bits (1309), Expect = 6.66e-182
Identity = 249/256 (97.27%), Postives = 254/256 (99.22%), Query Frame = 0


Query: 1   MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS 60
           MSSFRTAQI LVSSHREVYEPCDDSFALVDALLAD+VKLLNH+PTLCLEFGCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHNPTLCLEFGCGSGYVITS 60

Query: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL 120
           LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTD+ASGLEKRLAGSVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDLASGLEKRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLIS 240
            QICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPG+VVESLIS
Sbjct: 181 FQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240

Query: 241 QLPRLSFWRNNSNTSR 256
           QLPRLSFWRNNSNT+R
Sbjct: 241 QLPRLSFWRNNSNTNR 256

[Close]

BLAST of Cucsat.G9170 vs. ExPASy TrEMBL
Match: A0A1S3C053 (hemK methyltransferase family member 2 OS=Cucumis melo OX=3656 GN=LOC103495363 PE=3 SV=1)

HSP 1 Score: 508 bits (1309), Expect = 6.66e-182
Identity = 249/256 (97.27%), Postives = 254/256 (99.22%), Query Frame = 0


Query: 1   MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS 60
           MSSFRTAQI LVSSHREVYEPCDDSFALVDALLAD+VKLLNH+PTLCLEFGCGSGYVITS
Sbjct: 1   MSSFRTAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHNPTLCLEFGCGSGYVITS 60

Query: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL 120
           LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTD+ASGLEKRLAGSVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDLASGLEKRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLIS 240
            QICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPG+VVESLIS
Sbjct: 181 FQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240

Query: 241 QLPRLSFWRNNSNTSR 256
           QLPRLSFWRNNSNT+R
Sbjct: 241 QLPRLSFWRNNSNTNR 256

[Close]

BLAST of Cucsat.G9170 vs. ExPASy TrEMBL
Match: A0A5D3C4M9 (HemK methyltransferase family member 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001750 PE=3 SV=1)

HSP 1 Score: 506 bits (1303), Expect = 5.47e-181
Identity = 248/256 (96.88%), Postives = 253/256 (98.83%), Query Frame = 0


Query: 1   MSSFRTAQIPLVSSHREVYEPCDDSFALVDALLADQVKLLNHHPTLCLEFGCGSGYVITS 60
           MSSFR AQI LVSSHREVYEPCDDSFALVDALLAD+VKLLNH+PTLCLEFGCGSGYVITS
Sbjct: 1   MSSFRIAQIHLVSSHREVYEPCDDSFALVDALLADRVKLLNHNPTLCLEFGCGSGYVITS 60

Query: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDIASGLEKRLAGSVDVL 120
           LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTD+ASGLEKRLAGSVDVL
Sbjct: 61  LALILGKEASATHYIATDINPHAIRVTQETLEAHGLHAELVCTDLASGLEKRLAGSVDVL 120

Query: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180
           VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP
Sbjct: 121 VVNPPYVPTPEDEVGQDGIASAWAGGENGRSVIDRILPVADVLLSDKGWLYMVTLTANNP 180

Query: 181 AQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGKVVESLIS 240
            QICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPG+VVESLIS
Sbjct: 181 FQICLQMREKGYASRIVVQRSTEEESLHVIKFWKDADLQVDGKDSTHKTGPGRVVESLIS 240

Query: 241 QLPRLSFWRNNSNTSR 256
           QLPRLSFWRNNSNT+R
Sbjct: 241 QLPRLSFWRNNSNTNR 256

The following BLAST results are available for this feature:

BLAST of Cucsat.G9170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6SKR2	3.5e-42	46.23	Methyltransferase N6AMT1 OS=Mus musculus OX=10090 GN=N6amt1 PE=1 SV=1	[more]
Q9Y5N5	2.9e-41	45.96	Methyltransferase N6AMT1 OS=Homo sapiens OX=9606 GN=N6AMT1 PE=1 SV=4	[more]
Q9UT94	1.8e-22	33.48	eRF1 methyltransferase catalytic subunit mtq2 OS=Schizosaccharomyces pombe (stra...	[more]
Q58338	5.6e-16	27.00	Putative protein N5-glutamine methyltransferase MJ0928 OS=Methanocaldococcus jan...	[more]
O28490	8.9e-14	28.79	Putative protein N5-glutamine methyltransferase AF_1784 OS=Archaeoglobus fulgidu...	[more]

BLAST of Cucsat.G9170 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136904.1	9.00e-187	100.00	methyltransferase N6AMT1 [Cucumis sativus] >XP_011658842.1 methyltransferase N6A...	[more]
XP_008455111.1	1.38e-181	97.27	PREDICTED: hemK methyltransferase family member 2 [Cucumis melo] >ADN34260.1 N6-...	[more]
TYK06871.1	1.13e-180	96.88	hemK methyltransferase family member 2 [Cucumis melo var. makuwa]	[more]
XP_038888861.1	7.32e-177	94.53	methyltransferase N6AMT1 [Benincasa hispida]	[more]
XP_023553679.1	2.01e-174	93.36	hemK methyltransferase family member 2-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cucsat.G9170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K7J9	4.36e-187	100.00	MTS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067460 PE=3 SV...	[more]
A0A5A7SL12	6.66e-182	97.27	HemK methyltransferase family member 2 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
E5GCR1	6.66e-182	97.27	N6-DNA-methyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
A0A1S3C053	6.66e-182	97.27	hemK methyltransferase family member 2 OS=Cucumis melo OX=3656 GN=LOC103495363 P...	[more]
A0A5D3C4M9	5.47e-181	96.88	HemK methyltransferase family member 2 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007848	Methyltransferase small domain	PFAM	PF05175	MTS	coord: 43..129 e-value: 1.2E-10 score: 41.3
IPR004557	Eukaryotic/archaeal PrmC-related	TIGRFAM	TIGR00537	TIGR00537	coord: 18..213 e-value: 4.7E-27 score: 92.8
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 16..221 e-value: 5.4E-44 score: 151.8
None	No IPR available	PANTHER	PTHR45875	METHYLTRANSFERASE N6AMT1	coord: 4..221
None	No IPR available	PANTHER	PTHR45875:SF5	BNAC01G37640D PROTEIN	coord: 4..221
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 48..132 e-value: 9.33457E-8 score: 47.4247
IPR002052	DNA methylase, N-6 adenine-specific, conserved site	PROSITE	PS00092	N6_MTASE	coord: 120..126
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 25..200

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G9170.T1	Cucsat.G9170.T1	mRNA
Cucsat.G9170.T3	Cucsat.G9170.T3	mRNA
Cucsat.G9170.T2	Cucsat.G9170.T2	mRNA
Cucsat.G9170.T4	Cucsat.G9170.T4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006479	protein methylation
biological_process	GO:0032259	methylation
cellular_component	GO:0035657	eRF1 methyltransferase complex
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008276	protein methyltransferase activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity